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Bacterial spot, caused by Xanthomonas spp., is one of the most important diseases of tomato in Illinois. Field surveys were conducted during 2017–19 to assess occurrence of bacterial spot in commercial tomato fields. Severity of foliage and fruit infection was recorded, and symptomatic samples were collected from three-to-five cultivars in three different farms in each of northern, central, and southern regions of Illinois. Severity of symptomatic foliage ranged from 0% to 91% (average 36.7%) and incidence of symptomatic fruit ranges from 0% to 30% (average 10.8%). During the surveys, 266 Xanthomonas isolates were collected and identified as Xanthomonas gardneri and X. perforans using Xanthomonas-specific hrp primers. Eighty-six percent of the isolates from the northern region were identified as X. gardneri, whereas 73% of the isolates from southern region were identified as X. perforans. Isolates from the central region were identified as X. perforans and X. gardneri 53% and 47% of the time, respectively. Multilocus sequence analysis using six housekeeping genes (fusA, gap-1, gltA, gyrB, lepA, and lacF) revealed the endemic population of X. gardneri and X. perforans. In addition to Xanthomonas, nine non-Xanthomonas bacterial genera were isolated from the samples, with most of the isolates classified as Microbacterium, Pantoea, and Pseudomonas.