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Background: Documenting of indigenous knowledge related to traditional medicine often leads to discovery of new drugs and prevent from destruction and forgotten it. Objective: Documentation of medicinal use of Amygdalus lycioides Spach var. horrida in traditional medicine of local people Naein, Khoor and Biabanak county in northeast of Esfahan in Iran. Methods: Ethnopharmacological information was collected using snowball sampling, Participant Observation and semi-structured interviews from 120 informants. The question of the interviews includes demography of informants, plant local name, medicinal properties, symptoms of the disease, used part, mode of application, route of administration and effectiveness of medicine, Adverse Drug Reaction, Medications or alternatives for treatment, In addition, it was also investigated about whether the informants used it for therapeutic purposes. Results: In Traditional medicine of Case study the A.lycioides are used in the treatment of disease such as diabetes, blood fat, hypotensive, Inflammation of the prostate, durectic, kidney stone, pain, Constipation, Colds and asthma. 98% of informants have reached the desired medicinal properties using aerial parts of the species. Conclusion: This study report Medicinal properties of A. lycioides treating blood fat, Inflammation of the prostate, durectic, and kidney stone for the first time. Also the result shows that for medicinal uses it is not necessary to use the root of plant.