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Poisonous plants are a collective group of plants of various systematic categories that contain phytotoxins that pose a potential danger to humans and animals. A number of publications both in Russia and abroad are devoted to the problem of patterns of distribution of plants hazardous to human health in connection with environmental factors. This work is a continuation of research into the spread of plants dangerous to humans in Russia. The aim of this work is ecological and geographical analysis of poisonous plants distribution in biomes of the Far East in Russia. Resulted from the research work on the territory of the far-eastern biomes of Russia we revealed 87 the most toxic vascular plant species that belong to 21 plant families. Some of the most poisonous plants of the Russian flora are plants of the genus Aconitum, of the family (Ranunculaceae). About 70 species of this genus grow in Russia, of which 40 are found only in the Far East, since this territory is considered the center of botanical diversity of the genus in question. The cartographical analysis showed that the highest number of poisonous plant species could be found in the south-east regions – in Primorye, the basin of Ussuri river, in the lower and middle course of the river Amur. Based on a map of Russian biomes, optimal habitats for poisonous plants were identified. Maximum number of Aconitum species, as the most poisonous genus of Russian flora, is in the mountain biomes and in the plain forest-steppe biomes in the Amur basin. The types of ecosystems with the maximum abundance and diversity of poisonous plants have been identified. The species richness distribution shows the concentration of poisonous plant species in small mountain biomes and in arid-like biomes. Correlation analysis of relationships between the number of plant species and climatic factors revealed the significant closeness of the correlation with the average annual air temperature (0,66). Maximum correlation closeness appeared to be between number of poisonous plant species per 10 000 km2 and total number of vascular plants per 10 000 km2 (0,81).

The pharmacological experiments on isolated organs (ex vivo) are the preferred method for assessing the primary pharmacodynamics of the studied drugs, since this method is completely excluded the systemic influence of neurohumoral regulation. In the last decade, a new group of thrombolytic drugs based on immobilized subtilisins has been formed. At the stage of registrational preclinical and clinical studies, their pleiotropic pharmacological effects have not been studied. Meanwhile, there is a reason to consider that their pharmacological activity in the bloodstream is not limited to thrombolytic action, but may be extended to a systemic effect on the cardiovascular system. The aim of the study was to investigate the chronotropic effects of an isolated heart during its perfusion with solutions of immobilized subtilisins at different concentrations. Material and methods. The isolated rat heart model according to Langendorff was used in the study. The experiment included 50 Wistar rats, which were divided into 5 groups: isolated hearts perfused only with Krebs – Henseleit solution (control) or with immobilized subtilisins in 4 concentrations (170, 340, 510 и 1020 U/l). Results and discussion. The immobilized subtilisins have a negative chronotropic effect. The onset of the effect depends on the drug concentration in the solution: the higher concentration, the earlier effect. From 5 to 10 minutes of perfusion, a negative chronotropic effect is observed using of immobilized subtilisins at any dose. The duration of its increase is manifested up to 10–20 minutes, depending on the drug concentration in solution. After 20 minutes of perfusion, the achieved negative chronotropic effect remains at a plateau level up to 40 minutes. Conclusion. The immobilized subtilisins have an independent pharmacological effect on heart rate.