Search

The experiment investigated the effect of dietary orange pulp (OP) with or without enzymes supplementation on the performance parameters, chemical composition, serum biochemistry, and some liver gene expression in tilapia for twelve weeks. The studied fish (n = 300) were allotted into six groups according to OP level (0%, 10%, and 20%) with or without enzymes supplementation (+/-). The performance results indicated that the inclusion level of OP by 20% negatively affected FCR and decreased final body weight. The dietary enzymes did not alleviate the unfavorable effect of OP on the final body weight. Higher dietary replacement levels of OP reduced serum lipid profile, serum proteins, and carcass fat, but increased carcass protein and ash compared to the control. The dietary enzymes produced higher serum lipids and proteins as compared to non-supplemented groups. Fish-fed the control diet supplemented with enzymes (G2) expressed higher RNA levels of GHR and IGF1 genes compared to the other groups. Excess dietary OP lowered the RNA level of FAS and LPL genes but improved simultaneously when supplemented with enzymes. Overall, the higher dietary OP (20%) with or without enzymes supplementation, did not benefit the tilapia performance, metabolic, and genetic indices, and the chemical composition of the carcass. But, the lower level of OP (10%) supplemented with enzymes could be used in tilapia diets without adverse effects on performance, health, and carcass composition.

The present study aimed to assess the in ovo administration of clenbuterol on chick fertility, growth performance, muscle growth, myogenic gene expression, fatty acid, amino acid profile, intestinal morphology, and hepatic lipid-related gene expressions. In this study, 750 healthy fertile eggs from the local chicken breed Dokki-4 strain were analyzed. Fertile eggs were randomly divided into five experimental groups (150 eggs/3 replicates for each group). On day 14 of incubation, in addition to the control group, four other groups were established where 0.5 mL of worm saline (30 °C) was injected into the second group of eggs. In the third, fourth, and fifth groups, 0.5 mL of worm saline (30 °C), 0.9% of NaCl, and 10, 15, and 20 ppm of clenbuterol were injected into the eggs. Results suggested that clenbuterol increased growth efficiency up to 12 weeks of age, especially at 15 ppm, followed by 10 ppm, decreased abdominal body fat mass, and improved hatchability (p < 0.01). Clenbuterol also modulated saturated fatty acid levels in the breast muscles and improved essential amino acids when administered at 10 and 15 ppm. Additionally, clenbuterol at 15 ppm significantly decreased myostatin gene expression (p < 0.01) and considerably increased IGF1r and IGF-binding protein (IGFBP) expression. Clenbuterol administration led to a significant upregulation of hepatic PPARα, growth hormone receptor, and Lipoprotein lipase (LPL) mRNA expression with a marked decrease in fatty acid synthase (FAS) and sterol regulatory element-binding protein 1 (SREBP-1c) expression. In conclusion, the current study revealed that in ovo injection of clenbuterol showed positive effects on the growth of hatched chicks through reduced abdominal fat deposition, improved intestinal morphology, and modulation of hepatic gene expressions in myogenesis, lipogenesis, and lipolysis.

This study explored the impact of supplementation of L- carnitine on the performance of broiler chicken, biochemical parameters and expression of cationic amino acid transporter (CAT2), myogenic determining factor (MYOD) and myogenic factor 5 (MYF5) genes. This study included two groups; the first one is the control group and the second one received L-carnitine at a dose 50 mg/kg/day in drinking water. After 35 day, the sera and muscle tissue were obtained for biochemical and real time polymerase chain reaction (qPCR) analysis. The obtained results revealed a significant increase in the body weight, total body weight gain and a significant decrease in food consumption, food conversion rate in L-carnitine-supplemented group as compared to the control group. Moreover, there was no significant changes in serum cholesterol, triacylglycerol, AST, ALT, total protein and uric acid levels which showed a slight decrease in L-carnitine-supplemented group in comparison with the control group. This study also revealed a significant up regulation for the expression of CAT2, MYOD and MYF5 genes in muscles of broiler chicken-supplemented with L- carnitine as compared to the control one. The present study concluded that supplementation of L- carnitine to broiler chicken was useful to increase the total body weight gain and muscle mass and this beneficial effect was associated with up regulation of CAT2, MYOD, and MYF5 genes. Key words: L-carnitine; broiler chicken; growth traits; CAT2; MYOD; MYF5

The aim of current study was to determine the effect of dietary omega 3 fatty acids (FA) mainly eicosapentaenoic (EPA) and docosahexaenoic (DHA) on inflammatory response of post calving cows during transition to lactation. Twenty Holstein lactating cows were assigned into two groups (10 each). The two groups fed on the basal diet while one of them was supplemented with 70 g of omega-3 FA per cow. The supplement was added to the diet from the 1ST day of calving till the 60th days of lactation. Blood samples were taken 3 times weekly from the 2nd to the 4th week post calving. The blood samples were analyzed for glucose, insulin, non-esterified fatty acids (NEFA), beta hydroxyl butyric acid (BHBA), C- reactive protein (CRP), haptoglobin, albumin and globulin. Feeding omega-3 FAs significantly increased (P

The present study investigated the influence of altering omega 6 to omega 3 fatty acids (FA) ratio on growth, hemato- biochemical parameters and absorptive capacity of Nile tilapia. One hundred and fifty fingerlings (25.1±0.3 g), were assigned into 3 groups (5 replicates each) and fed on iso-caloric and iso-nitrogenous diets. Diets were supplemented with fish and soy oil to create 3 different ratio of omega 6 to omega 3 FA, namely, 0.91, 2.85 and 9.3 parts of omega 6 to 1 part of omega 3 (R1, R3 and R9, respectively). Increasing the ratio from R1 to R9 significantly decreased average daily gain and increased feed conversion ratio. Also this alteration decreased ash content in the carcass on the expense of ether extract. Number of erythrocytes, packed cell volume, hemoglobin concentration were significantly increased in fish fed R1. Feeding R9 increased heterophils and decreased lymphocytes as compared with R1 and R3. Fish fed R1 significantly had high serum protein and serum antibacterial activity (47.6%) than R9 (43.5%) and R3 (44.9%). Feeding R1 significantly decreased serum triglycerides, cholesterol and creatinine. Feeding R3 and R1 enhanced absorptive capacity of jejunum villi more than the group fed R9. Increasing the ratio of omega 6 to omega 3 FA from R1 to R9 decreased growth performance through decreasing absorptive capacity of jejunum villi. However, Feeding R3 had a positive effect on growth performance and absorptive capacity of the intestine of Nile tilapia. Key words: fish oil; growth performance; intestinal villi; Nile tilapia; soybean oil

Chicken Salmonella enterica serovars are enteric bacteria associated with massive public health risks and economic losses. There is a widespread antimicrobial resistance among S. enterica serotypes, and innovative solutions to antibiotic resistance are needed. We aimed to use probiotics to reduce antibiotic resistance and identify the major probiotic players that modify the early interactions between S. enterica and host cells. One-day-old cobb broiler chicks were challenged with S. typhimurium after oral inoculation with different probiotic strains for 3 days. The adherence of different probiotic strains to Caco-2 intestinal epithelial cells was studied in vitro. Lactobacillus (Lacticaseibacillus) casei ATTC334 and Bifidobacterium breve JCM1192 strains attached to Caco-2 cells stronger than B. infantis BL2416. L. casei ATTC334 and B. breve JCM1192 reduced S. typhimurium recovery from the cecal tonsils by competitive exclusion mechanism. Although B. infantis BL2416 bound poorly to Caco-2 epithelial cells, it reduced S. typhimurium recovery and increased IFN-γ and TNF-α production. L. casei ATTC334, B. breve JCM1192 and B. infantis BL2416 improved body weight gain and the food conversion rate in S. typhimurium-infected broilers. B. longum Ncc2785 neither attached to epithelial cells nor induced IFN-γ and TNF-α release and consequently did not prevent S. typhimurium colonization in broiler chickens. In conclusion, probiotics prevented the intestinal colonization of S. typhimurium in infected chickens by competitive exclusion or cytokine production mechanisms.