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10 results on '"Chiara Stefani"'

1. Utility of accessible SARS-CoV-2 specific immunoassays in vaccinated adults with a history of advanced HIV infection

Author

Ludovica Ferrari, Alessandra Ruggiero, Chiara Stefani, Livia Benedetti, Lorenzo Piermatteo, Eleonora Andreassi, Federica Caldara, Drieda Zace, Matteo Pagliari, Francesca Ceccherini-Silberstein, Christopher Jones, Marco Iannetta, Anna Maria Geretti, and The EVAN-COV Study Group

Subjects
Medicine, Science
Abstract

Abstract Accessible SARS-CoV-2-specific immunoassays may inform clinical management in people with HIV, particularly in case of persisting immunodysfunction. We prospectively studied their application in vaccine recipients with HIV, purposely including participants with a history of advanced HIV infection. Participants received one (n = 250), two (n = 249) or three (n = 42) doses of the BNT162b2 vaccine. Adverse events were documented through questionnaires. Sample collection occurred pre-vaccination and a median of 4 weeks post-second dose and 14 weeks post-third dose. Anti-spike and anti-nucleocapsid antibodies were measured with the Roche Elecsys chemiluminescence immunoassays. Neutralising activity was evaluated using the GenScript cPass surrogate virus neutralisation test, following validation against a Plaque Reduction Neutralization Test. T-cell reactivity was assessed with the Roche SARS-CoV-2 IFNγ release assay. Primary vaccination (2 doses) was well tolerated and elicited measurable anti-spike antibodies in 202/206 (98.0%) participants. Anti-spike titres varied widely, influenced by previous SARS-CoV-2 exposure, ethnicity, intravenous drug use, CD4 counts and HIV viremia as independent predictors. A third vaccine dose significantly boosted anti-spike and neutralising responses, reducing variability. Anti-spike titres > 15 U/mL correlated with neutralising activity in 136/144 paired samples (94.4%). Three participants with detectable anti-S antibodies did not develop cPass neutralising responses post-third dose, yet displayed SARS-CoV-2 specific IFNγ responses. SARS-CoV-2 vaccination is well-tolerated and immunogenic in adults with HIV, with responses improving post-third dose. Anti-spike antibodies serve as a reliable indicator of neutralising activity. Discordances between anti-spike and neutralising responses were accompanied by detectable IFN-γ responses, underlining the complexity of the immune response in this population.

Published
2024
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2. Implementation of the WHO standards to assess the quality of care for children with acute diarrhoea: findings of a multicentre study (CHOICE) in Italy

Author

Egidio Barbi, Silvia Bressan, Stefano Masi, Marzia Lazzerini, Paola Cogo, Vanna Graziani, Federico Marchetti, Giorgio Cozzi, Ilaria Mariani, Valentina Baltag, Ilaria Liguoro, Francesca Tirelli, Chiara Stefani, Sarah Contorno, Stefano Martelossi, Alessandro Amaddeo, Alessia Marcellino, Riccardo Lubrano, Enrico Felici, Annunziata Lucarelli, Gian Luca Trobia, Elia Balestra, Benmario Castaldo, Alice Del Colle, Chiara Pilotto, Paola Moras, Marcella Massarotto, Paola Crotti, Benedetta Ferro, Riccardo Pavanello, Silvia Fasoli, Chiara Grisaffi, Idanna Sforzi, Rosa Santangelo, Andrea Iuorio, Vanessa Martucci, Mariateresa Sanseviero, Roberta Parrino, Giuseppina de Rosa, Francesca Patané, Marta Minute, Fabio Cardinale, Alessandra Iacono, Angela Troisi, Marta Arrabito, Paolo Dalena, Massimo Dagnelut, Maristella Toniutti, Silvia Galiazzo, Maria Luisa Casciana, Marta Gagliardi, Kevin Valentino, Lucia Grazia Tricarico, Mariateresa De Sario, Alessandra Pisani, Maria Carla Finocchiaro, Laura Portale, Marco Greco, Emiliano Talanti, Anna Madera, Paola Stillo, Nicolò Chiti, Silvia Bloise, Silvia Sordelli, Mariangela Labruzzo, Massimo Lo Verde, Domenico Cipolla, Carlotta Farneti, Francesco Oppido, Giulia Sansovini, Giada Sartor, Marta Magnolato, Michele Maiola, Michela Pandullo, Sara Rivellini, Sara Dal Bo, and Vita Antonella Di Stefano

Subjects
Pediatrics, RJ1-570
Published
2024
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3. Evaluation of the WHO standards to assess quality of care for children with acute respiratory infections: findings of a baseline multicentre assessment (CHOICE) in Italy

Author

Egidio Barbi, Silvia Bressan, Stefano Masi, Marzia Lazzerini, Paola Cogo, Vanna Graziani, Federico Marchetti, Giorgio Cozzi, Ilaria Mariani, Ilaria Liguoro, Francesca Tirelli, Chiara Stefani, Sarah Contorno, Stefano Martelossi, Alessandro Amaddeo, Alessia Marcellino, Riccardo Lubrano, Enrico Felici, Annunziata Lucarelli, Gian Luca Trobia, Elia Balestra, Benmario Castaldo, Alice Del Colle, Chiara Pilotto, Paola Moras, Marcella Massarotto, Paola Crotti, Benedetta Ferro, Riccardo Pavanello, Silvia Fasoli, Chiara Grisaffi, Idanna Sforzi, Rosa Santangelo, Andrea Iuorio, Sara Dal Bo, Vanessa Martucci, Mariateresa Sanseviero, Roberta Parrino, Giuseppina de Rosa, Vita Antonella Di Stefano, Francesca Patané, Marta Minute, Fabio Cardinale, Alessandra Iacono, Angela Troisi, Marta Arrabito, Paolo Dalena, Massimo Dagnelut, Maristella Toniutti, Silvia Galiazzo, Maria Luisa Casciana, Marta Gagliardi, Kevin Valentino, Lucia Grazia Tricarico, Mariateresa De Sario, Alessandra Pisani, Maria Carla Finocchiaro, Laura Portale, Marco Greco, Emiliano Talanti, Anna Madera, Paola Stillo, Nicolò Chiti, Silvia Bloise, Silvia Sordelli, Mariangela Labruzzo, Massimo Lo Verde, Domenico Cipolla, Carlotta Farneti, Francesco Oppido, Giulia Sansovini, Giada Sartor, Marta Magnolato, Michele Maiola, Michela Pandullo, and Sara Rivellini

Subjects
Pediatrics, RJ1-570
Published
2024
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4. Implementation of the WHO Standards to assess quality of paediatric care at the facility level using service users’ perspective as source of data: a multicentre quality improvement study in Italy

Author

Egidio Barbi, Silvia Bressan, Stefano Masi, Marzia Lazzerini, Paola Cogo, Vanna Graziani, Federico Marchetti, Giorgio Cozzi, Ilaria Mariani, Valentina Baltag, Ilaria Liguoro, Francesca Tirelli, Chiara Stefani, Sarah Contorno, Stefano Martelossi, Alessandro Amaddeo, Alessia Marcellino, Riccardo Lubrano, Enrico Felici, Annunziata Lucarelli, Gian Luca Trobia, Elia Balestra, Benmario Castaldo, Alice Del Colle, Chiara Pilotto, Paola Moras, Marcella Massarotto, Paola Crotti, Benedetta Ferro, Riccardo Pavanello, Silvia Fasoli, Chiara Grisaffi, Idanna Sforzi, Rosa Santangelo, Andrea Iuorio, Sara Dal Bo, Vanessa Martucci, Mariateresa Sanseviero, Roberta Parrino, Giuseppina de Rosa, Vita Antonella Di Stefano, Francesca Patané, Marta Minute, Fabio Cardinale, Alessandra Iacono, Angela Troisi, Marta Arrabito, Paolo Dalena, Massimo Dagnelut, Maristella Toniutti, Silvia Galiazzo, Maria Luisa Casciana, Marta Gagliardi, Kevin Valentino, Lucia Grazia Tricarico, Mariateresa De Sario, Alessandra Pisani, Maria Carla Finocchiaro, Laura Portale, Marco Greco, Emiliano Talanti, Anna Madera, Paola Stillo, Nicolò Chiti, Silvia Bloise, Silvia Sordelli, Mariangela Labruzzo, Massimo Lo Verde, Domenico Cipolla, Carlotta Farneti, Francesco Oppido, Giulia Sansovini, Giada Sartor, Marta Magnolato, Michele Maiola, Michela Pandullo, Sara Rivellini, and Giuseppina De Rosa

Subjects
Pediatrics, RJ1-570
Published
2024
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5. Implementation of the WHO standards to assess quality of care for children with acute pain in EDs: findings of a multicentre study (CHOICE) in Italy

Author

Egidio Barbi, Silvia Bressan, Stefano Masi, Marzia Lazzerini, Paola Cogo, Vanna Graziani, Federico Marchetti, Giorgio Cozzi, Ilaria Mariani, Valentina Baltag, Ilaria Liguoro, Francesca Tirelli, Chiara Stefani, Sarah Contorno, Stefano Martelossi, Alessandro Amaddeo, Alessia Marcellino, Riccardo Lubrano, Enrico Felici, Annunziata Lucarelli, Gian Luca Trobia, Elia Balestra, Benmario Castaldo, Alice Del Colle, Chiara Pilotto, Paola Moras, Marcella Massarotto, Paola Crotti, Benedetta Ferro, Riccardo Pavanello, Silvia Fasoli, Chiara Grisaffi, Idanna Sforzi, Rosa Santangelo, Andrea Iuorio, Sara Dal Bo, Vanessa Martucci, Mariateresa Sanseviero, Roberta Parrino, Giuseppina de Rosa, Vita Antonella Di Stefano, Francesca Patané, Marta Minute, Fabio Cardinale, Alessandra Iacono, Angela Troisi, Marta Arrabito, Paolo Dalena, Massimo Dagnelut, Maristella Toniutti, Silvia Galiazzo, Maria Luisa Casciana, Marta Gagliardi, Kevin Valentino, Lucia Grazia Tricarico, Mariateresa De Sario, Alessandra Pisani, Maria Carla Finocchiaro, Laura Portale, Marco Greco, Emiliano Talanti, Anna Madera, Paola Stillo, Nicolò Chiti, Silvia Bloise, Silvia Sordelli, Mariangela Labruzzo, Massimo Lo Verde, Domenico Cipolla, Carlotta Farneti, Francesco Oppido, Giulia Sansovini, Giada Sartor, Marta Magnolato, Michele Maiola, Michela Pandullo, and Sara Rivellini

Subjects
Pediatrics, RJ1-570
Published
2024
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6. Implementation of the WHO Standards to assess the quality of paediatric care using health workers as source of data: findings of a multicentre study (CHOICE) in Italy

Author

Egidio Barbi, Silvia Bressan, Stefano Masi, Marzia Lazzerini, Paola Cogo, Vanna Graziani, Federico Marchetti, Ilaria Mariani, Valentina Baltag, Ilaria Liguoro, Francesca Tirelli, Chiara Stefani, Sarah Contorno, Stefano Martelossi, Alessia Marcellino, Riccardo Lubrano, Enrico Felici, Annunziata Lucarelli, Gian Luca Trobia, Elia Balestra, Benmario Castaldo, Alice Del Colle, Chiara Pilotto, Paola Moras, Marcella Massarotto, Paola Crotti, Benedetta Ferro, Riccardo Pavanello, Silvia Fasoli, Chiara Grisaffi, Idanna Sforzi, Rosa Santangelo, Andrea Iuorio, Vanessa Martucci, Mariateresa Sanseviero, Roberta Parrino, Giuseppina de Rosa, Vita Antonella Di Stefano, Francesca Patané, Marta Minute, Fabio Cardinale, Alessandra Iacono, Marta Arrabito, Paolo Dalena, Massimo Dagnelut, Maristella Toniutti, Silvia Galiazzo, Maria Luisa Casciana, Marta Gagliardi, Kevin Valentino, Lucia Grazia Tricarico, Mariateresa De Sario, Alessandra Pisani, Maria Carla Finocchiaro, Laura Portale, Marco Greco, Emiliano Talanti, Anna Madera, Paola Stillo, Nicolò Chiti, Silvia Bloise, Silvia Sordelli, Mariangela Labruzzo, Massimo Lo Verde, Domenico Cipolla, Carlotta Farneti, Francesco Oppido, Giulia Sansovini, Giada Sartor, Marta Magnolato, Michele Maiola, Michela Pandullo, Sara Rivellini, Sara Dal Bo, Giuseppina De Rosa, Dal Bo Sara, Troisi Angela, Cozzi Giorgio, and Amaddeo Alessandro

Subjects
Pediatrics, RJ1-570
Published
2024
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7. WHO standards-based tools to measure service providers’ and service users’ views on the quality of hospital child care: development and validation in Italy

Author

Egidio Barbi, Marta Arpone, Silvia Bressan, Marzia Lazzerini, Emanuelle Pessa Valente, Paola Cogo, Federico Marchetti, Giorgio Cozzi, Francesco Tagliaferri, Ilaria Mariani, Valentina Baltag, Francesca Peri, Ilaria Liguoro, Chiara Stefani, Claudio Germani, Silvana Schreiber, Stefano Martelossi, Angelika Velkoski, Alessandro Amaddeo, Alessia Marcellino, Riccardo Lubrano, Moise Muzigaba, Tereza Rebecca de Melo e Lima, Enrico Felici, Annunziata Lucarelli, Gian Luca Trobia, Daniela Nisticò, Wilson Milton Were, Elia Balestra, Benmario Castaldo, Alice Del Colle, De Monte Roberta Coordinatrice, Tamara Strajn Coordinatrice, Livia Bicego, Andrea Cassone, Chiara Pilotto, Lisa Stavro, Paola Moras, Marcella Massarotto, Paola Crotti, Benedetta Ferro, Riccardo Pavanello, Silvia Fasoli, Pelazza Carolina, Marta Coppola, Chiara Grisaffi, Elisabetta Mingoia, Idanna Sforzi, Rosa Santangelo, Andrea Iuorio, Sara Dal Bo, Vanessa Martucci, Mariateresa Sanseviero, Bloise Silvia, Eleonora Canzio, Roberta Parrino, Salvatore Gambino, Melania Guardino, Luca Lagalla, Beatrice Vaccaro, Giuseppina de Rosa, Vita Antonella Di Stefano, and Francesca Patané

Subjects
Medicine
Abstract

Objectives Evidence showed that, even in high-income countries, children and adolescents may not receive high quality of care (QOC). We describe the development and initial validation, in Italy, of two WHO standards-based questionnaires to conduct an assessment of QOC for children and young adolescents at inpatient level, based on the provider and user perspectives.Design Multiphase, mixed-methods study.Setting, participants and methods The two questionnaires were developed in four phases equally conducted for each tool. Phase 1 which included the prioritisation of the WHO Quality Measures according to predefined criteria and the development of the draft questionnaires. In phase 2 content face validation of the draft questionnaires was assessed among both experts and end-users. In phase 3 the optimised questionnaires were field tested to assess acceptability, perceived utility and comprehensiveness (N=163 end-users). In phase 4 intrarater reliability and internal consistency were evaluated (N=170 and N=301 end-users, respectively).Results The final questionnaires included 150 WHO Quality Measures. Observed face validity was excellent (kappa value of 1). The field test resulted in response rates of 98% and 76% for service users and health providers, respectively. Among respondents, 96.9% service users and 90.4% providers rated the questionnaires as useful, and 86.9% and 93.9%, respectively rated them as comprehensive. Intrarater reliability was good, with Cohen’s kappa values exceeding 0.70. Cronbach alpha values ranged from 0.83 to 0.95, indicating excellent internal consistency.Conclusions Study findings suggest these tools developed have good content and face validity, high acceptability and perceived utility, and good intrarater reliability and internal consistency, and therefore could be used in health facilities in Italy and similar contexts. Priority areas for future research include how tools measuring paediatric QOC can be more effectively used to help health professionals provide the best possible care.

Published
2022
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8. HIV and SARS-CoV-2 Co-Infection: From Population Study Evidence to In Vitro Studies

Author

Chiara Stefani, Tobia Fantoni, Michele Bissoli, Jordan Thomas, and Alessandra Ruggiero

Subjects
HIV-1, SARS-CoV-2, co-infections, anti-retroviral drugs, Science
Abstract

Human immunodeficiency virus type 1 (HIV-1) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have caused two major viral outbreaks during the last century. Two major aspects of HIV-1 and SARS-CoV-2 co-infection have been extensively investigated and deserve attention. First, the impact of the co-infection on the progression of disease caused by HIV-1 or SARS-CoV-2. Second, the impact of the HIV-1 anti-retroviral treatment on SARS-CoV-2 infection. In this review, we aim to summarize and discuss the works produced since the beginning of the SARS-CoV-2 pandemic ranging from clinical studies to in vitro experiments in the context of co-infection and drug development.

Published
2022
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9. Increased Prevalence of Unstable HLA-C Variants in HIV-1 Rapid-Progressor Patients

Author

Chiara Stefani, Antonella Sangalli, Elena Locatelli, Tania Federico, Giovanni Malerba, Maria Grazia Romanelli, Gustavo Adolfo Argañaraz, Bosco Christiano Maciel Da Silva, Alberto Jose Duarte Da Silva, Jorge Casseb, Enrique Roberto Argañaraz, Alessandra Ruggiero, and Donato Zipeto

Subjects
AIDS progression, HLA-C stability, HIV-1 infection control, ASPCR, MHC class I, Biology (General), QH301-705.5, Chemistry, QD1-999
Abstract

HIV-1 infection in the absence of treatment results in progression toward AIDS. Host genetic factors play a role in HIV-1 pathogenesis, but complete knowledge is not yet available. Since less-expressed HLA-C variants are associated with poor HIV-1 control and unstable HLA-C variants are associated with higher HIV-1 infectivity, we investigated whether there was a correlation between the different stages of HIV-1 progression and the presence of specific HLA-C allotypes. HLA-C genotyping was performed using allele-specific PCR by analyzing a treatment-naïve cohort of 96 HIV-1-infected patients from multicentric cohorts in the USA, Canada, and Brazil. HIV-1-positive subjects were classified according to their different disease progression status as progressors (Ps, n = 48), long-term non-progressors (LTNPs, n = 37), and elite controllers (ECs, n = 11). HLA-C variants were classified as stable or unstable according to their binding stability to β2-microglobulin/peptide complex. Our results showed a significant correlation between rapid progression to AIDS and the presence of two or one unstable HLA-C variants (p-value: 0.0078, p-value: 0.0143, respectively). These findings strongly suggest a link between unstable HLA-C variants both at genotype and at allele levels and rapid progression to AIDS. This work provides further insights into the impact of host genetic factors on AIDS progression.

Published
2022
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10. Thermomechanical Analysis of a PFC Integrating W Lattice Armour in Response to Different Plasma Scenarios Predicted in the EU-DEMO Tokamak

Author

Damiano Paoletti, Pierluigi Fanelli, Riccardo De Luca, Chiara Stefanini, Francesco Vivio, Valerio Gioachino Belardi, Simone Trupiano, Giuseppe Calabrò, Jeong-Ha You, and Rudolf Neu

Subjects
EU-DEMO, limiter, PFC, lattice, finite element, Nuclear engineering. Atomic power, TK9001-9401
Abstract

Despite the high performance exhibited by tungsten (W), no material would be able to withstand the huge loads expected with extreme plasma transients in EU-DEMO and future reactors, where the installation of sacrificial first wall limiters is essential to prevent excessive wall degradation. The integration of W lattices in the architecture of such components can allow for meeting their conflictual requirements: indeed, they must ensure the effective exhaust of the nominal thermal load during stationary operation; when transients occur, they must thermally insulate and decouple the surface from the heat sink, promoting prompt vapour shielding formation. Starting from the optimised layouts highlighted in a previous study, in this work, a detailed 3D finite element model was developed to analyse in depth the influence of the actual features of the latticed metamaterial on the overall performance of the EU-DEMO limiter PFC on the basis of a flat tile configuration. Its main goal is to help in identifying the most promising layout as a preconceptual design for the fabrication of a small-scale mock-up. For this purpose, the complex geometry of a W-based lattice armour was faithfully reproduced in the model and analysed. This allowed for a detailed assessment of the thermally induced stresses that develop in the component because of the temperature field in response to a number of plasma scenarios—above all, normal operation and ramp down. Structural integrity was verified through the acceptance criteria established for ITER. The two optimised layouts proposed for the PFC were able to effectively meet the requirements under normal reactor operating conditions, while they missed some requirements in the ramp-down case. However, the first HHF tests will be performed in order to benchmark the analyses.

Published
2022
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