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Land-use in complex urban regions and their future development is uncertain, especially in view of (global) change conditions and the planetary boundaries discussed. An encompassing discussion and weighting within planning is necessary, on what ecosystems offer which kind of services to urban and regional societies and how this current supply and further potentials can be sustainably used. This is essential to contribute to a resilient urban and regional development. Scientific discussions on the role of ecosystem services in spatial planning do partly investigate the current status-quo of references to ecosystem services. This paper presents results from two German case study regions on the extent of references to ecosystem services in existing spatial planning instruments. Both binding as well as non-binding regional and urban development concepts were considered. Although none of the planning instruments explicitly uses the term “ecosystem services”, references to ecosystem services can be identified in all instruments. However, the majority of classified ecosystem services is not linked to specific objectives. This evokes questions on if spatial planning is oriented on keeping options open to act in the future and what the detected blind spots tell about the interpretation of the vision of sustainable spatial development.

Hydrogels are attractive biomaterials because their chemical and mechanical properties can be tailored to mimic those of biological tissues. However, many hydrogels do not allow cell or protein attachment. Therefore, they are post-synthetically functionalized by adding functional groups for protein binding, which then allows cell adhesion in cell culture substrates. However, the degree of functionalization and covalent binding is difficult to analyze in these cases. Moreover, the density of the functional groups and the homogeneity of their distribution is hard to control. This work introduces another strategy for the biofunctionalization of hydrogels: we synthesized a polymerizable linker that serves as a direct junction between the polymeric structure and cell adhesion proteins. This maleimide-containing, polymerizable bio-linker was copolymerized with non-functionalized monomers to produce a bioactive hydrogel based on poly(2-hydroxyethyl methacrylate) (pHEMA). Therefore, the attachment site was only controlled by the polymerization process and was thus uniformly distributed throughout the hydrogel. In this way, the bio-conjugation by a protein-binding thiol-maleimide Michael-type reaction was possible in the entire hydrogel matrix. This approach enabled a straightforward and highly effective biofunctionalization of pHEMA with the adhesion protein fibronectin. The bioactivity of the materials was demonstrated by the successful adhesion of fibroblast cells.