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目的构建131I标记的肝癌核酸纳米火车（NT），并探讨其作为肝癌靶向新型核素载体的可能性。方法三条核酸短链经退火处理后自组装形成核酸长链并采用氯胺T法进行放射性碘标记得到131Ⅰ-NT，纸层析法测纳米粒子的标记率及放射化学纯度，检测标记产物在不同温度（4 ℃、室温）及不同的储存溶剂（PBS、纯血清）中的体外稳定性。通过激光共聚焦显微镜检测肝癌细胞对纳米粒子的特异性摄取情况，分别测定131Ⅰ-NT与人肝癌细胞HepG2、正常肝细胞 L02 结合后细胞的放射性摄取率。通过尾静脉注射131Ⅰ-NT至HepG2荷瘤小鼠体内，进行生物分布研究。结果131Ⅰ-NT标记率为（93.05±0.74）%，纯化后放化纯度为（98.35±0.32）%。4 ℃储存条件下，标记产物在PBS和纯血清中24 h后的放化纯度分别为（92.77±0.04）%、（89.43±0.2）%。131Ⅰ-NT分别与两种细胞孵育2 h后，HepG2细胞的放射性摄取率明显高于L02细胞。尾静脉注射131Ⅰ-NT后，荷瘤小鼠体内 30 min、1 h、2 h肿瘤部位每克组织放射性摄取值分别为（4.90±0.55）%ID/g、（10.12±0.32）%ID/g、（4.25±0.31）%ID/g，T/M比值相应为7.33±2.04、36.54±12.72、44.93±7.90。结论成功构建了131I标记的长链核酸纳米火车，具有较好体外稳定性，对HepG2细胞及HepG2细胞荷瘤小鼠模型具有较高的靶向性，显示131Ⅰ-NT可能是一种具有潜力的靶向人肝癌的核素载体，为肝癌靶向核素诊疗提供新思路。

提出一种新型模块装配式灌浆钢梁柱节点，其上、下模块的梁通过螺栓连接，而方钢管柱通过内套承插钢管连接，且钢管柱内壁与承插管外壁设置抗剪键并灌浆。为了研究所提出节点的滞回性能、破坏模式、节点承载力以及节点的转动能力，对2个节点试件进行了低周往复加载试验，结果表明：两试件均发生钢管柱局部屈曲，并最终在梁柱焊接处钢管柱发生开裂；该新型节点的承载力、延性以及耗能能力与比对组未灌浆承插节点基本相同，同时灌浆节点试件的塑性转角满足美国抗震规范的限值要求，表明灌浆节点具有良好的抗震性能。

目的研究不同类型沙眼衣原体蛋白在输卵管性不孕中的致病作用，预测具有临床检测价值的沙眼衣原体蛋白抗体。方法本研究共纳入输卵管性不孕组（TFI）58例，正常生育组（FC）41例，非输卵管性不孕组（IFC）18例。血清检测CT-IgG ELISA试剂盒检测三组人群血清中CT-IgG的表达情况；利用前期获得表达、纯化的6种沙眼衣原体蛋白，建立这6种沙眼衣原体蛋白抗体的ELISA检测方法，分别检测6种沙眼衣原体蛋白抗体在TFI组、FC组和IFC组血清中的表达情况；对三组研究对象6种沙眼衣原体蛋白抗体的OD值进行统计描述，以CT-IgG的检测结果（阳/阴）为参考标准，绘制6种沙眼衣原体蛋白抗体的受试者工作特征曲线，利用约登指数确定各抗体的截断值。以TFI作为参考类，将FC、IFC分别与参考类建立两个无序多分类logistic回归模型，探究沙眼衣原体蛋白抗体、年龄预测TFI、FC和IFC的价值，并利用后退法筛选变量。结果各组研究对象CT376和CT443抗体OD值不全相同，CT-IgG阳性率不全相同。经两两比较发现，TFI组的CT376抗体OD值高于FC组（0.86 vs. 0.60，P=0.026）。TFI组的CT376抗体OD值高于IFC组（0.86 vs. 0.64，P=0.026）。IFC组CT443抗体OD值高于TFI组（0.59 vs. 0.34，P=0.036），高于FC组（0.59 vs. 0.30，P=0.02）。经过比较，CT376抗体的阳性率在三组间不全相同（PP P=0.068， OR=0.359，95%CI（0.120，1.078）］。CT-IgG是输卵管性不孕发生的独立危险因素，CT376抗体尚不能成为输卵管性不孕的独立危险因素；TFI与IFC建立的无序多分类logistic回归分析显示，在不孕的患者中，CT-IgG ［PPP

对中、美、欧现行规范关于钢结构截面类型及宽厚比限值的要求进行了归纳比较,利用ABAQUS有限元软件进行了单根H形钢梁考虑初始缺陷的屈曲承载力分析并与规范公式计算结果进行了对比。在此基础之上,以雄安站站房雨棚钢结构为背景,选取具有代表性的区域建立空间交叉H形钢梁精细有限元模型。对不同板件宽厚比的空间交叉H形钢梁进行了非线性屈曲承载力分析,研究了翼缘宽厚比及腹板高厚比对空间交叉H形钢梁局部稳定性和屈曲性能的影响。研究结果表明:ABAQUS模型对钢梁整体稳定性计算结果与规范公式吻合度较高,设计中采用杆件单元模型进行结构整体稳定性分析是安全合理的。考虑翼缘约束作用的H形钢梁腹板屈曲计算模型与该项目交叉钢梁有限元弹性屈曲分析结果吻合良好,结构起坡所引起的不均匀正应力是导致钢梁腹板失稳的主要因素。翼缘厚度确定后,根据上翼缘失稳与腹板局部屈曲临界值确定腹板厚度,依据S3级截面要求所控制的次梁腹板厚度是经济合理的。

为了减小钢框架梁柱弱轴连接中柱腹板上的应力水平,提出了在梁端加过渡竖板的方式以使得柱翼缘更好地参与受力。同时为了使塑性铰不出现在梁柱连接焊缝处,对梁翼缘进行削弱处理,最终提出了一种新型的梁端加强与削弱并用的梁柱弱轴连接(SWWC)形式,并运用有限元软件ABAQUS对已有的RBS型工字形柱弱轴连接节点的试验进行模拟验证后,对此类节点进行循环荷载作用下的受力性能分析。结果表明:在梁端加过渡竖板可以明显降低节点域的Mises应力水平,使得梁截面的屈曲主要集中在竖向板的外侧;在梁端加过渡竖板的同时对梁翼缘进行削弱处理可以使塑性铰外移;随着削弱深度c的增加,节点的承载力逐渐降低,RBS削弱处的屈曲发展得越充分,削弱起始位置至梁端的距离a和削弱区域的长度b对节点的承载力影响会很小,随着参数a或b的增加,梁上塑性铰中心逐渐远离梁翼缘焊缝处;建议削弱参数c=0.17bf,a取值范围为0.65bf～0.70bf,b=0.75hb,其中bf和hb分别为梁翼缘宽度和梁截面高度。

基于密集波分复用（DWDM）的光传送网构成了整个通信网络的基础物理层。为了满足DWDM技术的应用和升级,能够进行在线监测的光通道性能模块成为当前很多光器件公司研究和开发的热点。一个典型的在线光性能监测（OPM）模块能够在线监测通道光功率、中心波长及光信噪比（OSNR）等指标。文章系统地阐述了OPM的应用背景、技术特点、性能指标以及发展前景,并对各厂家生产的OPM模块进行了比较分析。

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为提高餐饮废油生物柴油的品质，降低其甘油和硫含量，分析了离心、静置、水洗-旋蒸3种方法的脱甘油效果，以及氧化、吸附（活性炭作吸附剂）2种方法的脱硫效果，并对甘油和硫含量进行同步优化研究。结果表明：在3种脱甘油方法中，水洗-旋蒸的脱甘油效果最好；氧化脱硫在将硫化物脱除的同时会导致生物柴油的损失，并降低生物柴油的氧化稳定性，与静置和超声氧化脱硫相比，搅拌氧化脱硫的效果较好，脱硫率和生物柴油损失率分别为28.5%和2.0%；在活性炭用量为2%时，脱硫率较高，为23.8%；水洗-旋蒸可以有效地对生物柴油中甘油和硫同时脱除，脱除后的硫、甘油单酯、游离甘油、总甘油含量分别为9.31 mg/kg、0.182%、0.008%、0.054%，脱除率分别达到11.9%、64.3%、78.9%和68.4%，符合GB 25199—2017附录C中BD100生物柴油标准要求。因此，实际生产中可以采用水洗-旋蒸的方法对生物柴油中的甘油和硫进行同步脱除。In order to improve the quality of biodiesel prepared from waste cooking oil and reduce its glycerol and sulfur content, the deglycerol effects of centrifugation, standing, water washing-rotary evaporation, as well as the desulfurization effects of oxidation and adsorption (using activated carbon as adsorbent) were analyzed, and the glycerol and sulfur content were synchronously optimized. The results showed that among the three methods of glycerol removal, water washing-rotary evaporation had the best effect. Oxidative desulfurization could lead to the loss of biodiesel while removing sulfides, and reduce the oxidation stability of biodiesel. Compared with standing and ultrasonic oxidation desulfurization, stirring oxidation desulfurization had a better effect, and the desulfurization rate and biodiesel loss rate were 285% and 2.0%, respectively. When the amount of activated carbon was 2%, the desulfurization rate was relatively high, reaching 23.8%. Water washing-rotary evaporation could effectively remove both glycerol and sulfur simultaneously. The contents of sulfur, monoglyceride, free glycerol, total glycerol in the removed biodiesel were 9.31 mg/kg, 0.182%, 0.008% and 0054%, respectively, and the removal rates were 11.9%, 64.3%, 78.9% and 68.4%, respectively, which met the requirements of BD100 biodiesel standard in GB 25199-2017 appendix C. Therefore, in actual production, the water washing-rotary evaporation method can be used to simultaneously remove glycerol and sulfur from biodiesel.

Objective To investigate the effect of quality control circle (QCC)activities in improving the standardized implementation rate of peripheral venous thrombolysis for lower-limb deep venous thrombosis (DVT). Methods A QCC group was set up. Factors causing failed implementation rate of DVT were analyzed, and countermeasures were carried out for continuous quality improvement. Results The standardized implementation rate of peripheral venous thrombolysis for lower-limb DVT increased form 33. 33% before QCC activities to 91. 25% after QCC activities, with a significant difference (P＜0. 05). Conclusion The implementation of QCC activities for continuous quality improvement of standardized procedure of peripheral venous thrombolysis may help to improve the effect of thrombolytic therapy. (目的 探讨品管圈在提高下肢深静脉血栓形成(DVT)患者外周静脉溶栓执行规范率中的效果。方法 成立品管圈小组, 进行品管圈知识培训, 选定活动主题, 进行现状调查, 分析下肢DVT患者外周静脉溶栓执行不合格原因, 制定对策并实施质量持续改进。结果 下肢DVT患者外周静脉溶栓执行规范率由品管圈活动前的33. 33%提高至开展品管圈活动后的91. 25%, 差异有统计学意义(P＜0. 05)。结论 开展品管圈活动, 对下肢DVT患者外周静脉溶栓执行规范率进行持续改进, 可以有效提高溶栓效果。)

This article summarizes the experience of Traditional Chinese Medicine(TCM) nursing appropriate techniques for a diabetic patient with back carbuncle treated with vacuum sealing drainage (VSD) technology. Efforts have been made to enhance the continuous vacuum sealing drainage. Based on the TCM theory, nursing appropriate techniques such as auricular acupoint pressing, intradermal needling, TCM oral care, TCM five-tone therapy were carried out for management of blood pressure and diabetes mellitus, reduce the incidence the infection and improve the wound healing. (本文总结1例负压封闭引流技术(VSD)联合中医适宜护理操作技术治疗糖尿病合并背部外痈患者的护理经验。术前做好充分准备, 术后做好VSD持续引流的观察与护理。基于中医基础理论, 采用耳穴刺激、皮内针刺激、中药漱口液清洁口腔、中医五音疗法等中医护理适宜技术, 有效控制高血压、糖尿病等基础疾病, 降低感染风险, 促进创面的愈合。)

目的 探讨心源性卒中患者使用丁苯酞对华法林抗凝作用的影响。 方法 前瞻性连续纳入成都医学院第一附属医院神经内科2019年6月—2021年12月期间收治的心源性卒中患者，患者入组后随机分为试验组（华法林+丁苯酞）和对照组（华法林）。监测两组患者的国际标准化比值（international standardized ratio，INR）变化情况并收集两组患者的临床资料，比较分析两组患者的INR达标时间、2周内达标率和90 d mRS评分的差异。 结果 本研究共纳入101例心源性卒中患者，试验组51例，对照组50例。两组基线数据（年龄、性别、用药前INR、收缩压和舒张压）差异无统计学意义，入院时试验组的NIHSS评分[10（3.0～15.0）分 vs. 0（0～6.5）分，P

Dynamic blood glucose monitoring system (CGMS) can monitor the blood glucose of patients with diabetes for many consecutive days and provide dynamic blood glucose monitoring maps, it has been widely used in clinic, which provides reliable data for doctors to understand the blood glucose fluctuations of patients, especially asymptomatic hypoglycemia at night. This paper reviewed the studies about principle of CGMS, application advantages in the treatment of type 2 diabetes mellitus and application status in the clinical setting, in order to provide reference to the treatment of nursing of patients with type 2 diabetes mellitus. (动态血糖监测系统(CGMS)可对糖尿病患者实行连续的血糖监测, 提供动态血糖图谱, 为医务人员的诊疗提供可靠的依据。本研究就CGMS的原理、在T2DM患者治疗中的应用优势和现状进行综述, 旨在为T2DM患者的治疗和护理提供可靠参考。)

目的 探讨融合声音与运动传感器的步态分析技术的可靠性及敏感性。 方法 选取47名受试者进行步态分析，其中包括22名偏瘫患者及25名健康受试者，对其运动学参数 进行记录和比较，以检测该步态分析系统的敏感性。由一名评定者对两组受试者分别进行2次步态 测试，采用相关系数进行重测信度比较。 结果 病例组与对照组受试者的步幅、步速、步频、摆动相时间、支撑相时间、双足支撑时间及踝 关节角度等运动学参数比较，差异均具有统计学意义。应用融合声音与运动传感器的步态分析技术2 次测量所得各项步态参数的组内相关系数均较高（0.901～0.996），提示重测信度好。 结论 融合声音与运动传感器的步态分析技术对于步行能力的评估具有较好的敏感性及稳定性。 Abstract: Objective To investigate the reliability and sensitivity of the gait analysis system based on the sound and motion sensors. Methods 47 subjects were recruited in this gait analysis, including 22 stroke patients with hemiplegia and 25 healthy subjects. The gait parameters of all the subjects were recorded and compared to investigate the sensitivity of this system. The subjects were detected twice by the same doctor, and the intraclass correlation coefficient (ICC) was used to evaluate the reliability of the gait analysis system. Results The kinematic parameters (stride length, walking speed, walking frequency, stride period, stance duration, double support duration and the angle of ankle) of the two groups showed significant differences. ICCs values of the gait parameters ranged from 0.901 to 0.996, which suggested a good test-retest reliability. Conclusions The gait analysis system based on the sound and motion sensors has a good sensitivity and reliability.

We study transport in twisted bilayer graphene and show that electrostatic barriers can act as valley splitters, where electrons from the K (K^{′}) valley are transmitted only to, e.g., the top (bottom) layer, leading to valley-layer locked currents. We show that such a valley splitter is obtained when the barrier varies slowly on the moiré scale and induces a Lifshitz transition across the junction, i.e., a change in the Fermi-surface topology. Furthermore, we show that for a given valley the reflected and transmitted current are transversely deflected, as time-reversal symmetry is effectively broken in each valley separately, resulting in valley-selective transverse focusing at zero magnetic field.

基于Micaps常规资料、NCEP再分析资料和中尺度自动站雨量资料，结合WRF高精度数值模拟输出的诊断物理量，研究了 2013年10月7日20：00至8日08：00发生于浙江东北部的短时大暴雨天气过程的成因.结果表明：这次大暴雨过程主要由台风“菲特”残留云系、超强台风“丹娜丝”和北方的弱冷空气共同影响产生；假相当位温及地面风场的分布很好地反映了此次大暴雨过程中的不稳定强对流及冷暖空气交汇的形势.垂直螺旋度、湿位涡的分布及演变情况与强降水的移动及强度变化有很好的对应关系，暴雨区上空螺旋度对应上负下正的垂直分布形势，低层的湿位涡分布与暴雨中心对应较好，对暴雨落区和暴雨中心有一定的指向作用.这对今后预报秋季台风外围大暴雨过程有指示意义.