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The lesser grain borer (Rhyzopertha dominica F.) and the rice weevil (Sitophilus oryzae L.) are stored grain pests that cause significant economic losses in grain storage. This study aimed to analyze the impact of the chemical composition of wheat and barley grain (e.g., protein, fatty acids and total antioxidant capacity) on the development of two species of storage pests and to determine the relationship between the analyzed variables. The study involved the evaluation of 10 wheat cultivars and 10 barley cultivars under laboratory conditions. The observations included assessing the beetles’ progeny abundance, dust mass produced after feeding, and grain mass loss. The chemical composition of the tested wheat and barley cultivars was also determined, and the influence of different chemical compositions on insect development was investigated. The results of the experiment revealed diversity of resistance among cultivars to pest feeding. Larger populations of the lesser grain borer were observed on barley grains, while rice weevil populations were higher on wheat. Cultivars with higher protein and fat content were more susceptible to pest attacks. A connection between the amount of dust, grain mass loss, and the type of pest was also identified, indicating differences in feeding mechanisms and selective food preferences of these insects. The grain chemistry of wheat cultivars, including the content of fatty acids and antioxidants, significantly influenced the progeny abundance of S. oryzae, suggesting the potential of these components as natural barriers against storage pests. This study provides valuable insights for developing breeding strategies to enhance the natural resistance of new grain cultivars to these pests, contributing to the reduction of pesticide use. Statistical analyses confirmed the significance of differences in grain composition in varied resistance to the studied pests. The conclusions drawn from this work may help establish new storage and breeding practices, promoting sustainable agriculture and protecting natural resources.

Abstract Background Heat stress is one of the abiotic stresses that make wheat crops vulnerable, which significantly impacts crop production around the world. An increase in temperature during the reproductive phase (anthesis) beyond the optimal range of 15–20 °C leads to decreased crop production, poor quality of the grain, and altered physiological and biochemical processes. To study the association between high temperature and physio-biochemical traits under normal and late sown, a set of fifteen genotypes was utilized. Results Relative water content under high temperatures had an overall decrease of 8.7%. However, grain protein and malondialdehyde content were higher in the stressed conditions than in the control, with increases of 20.2% and 38.9%, respectively. Marker Xgwm67, located on chromosome 5B, was found to be significantly associated with malondialdehyde content (R 2 = 21%) and Xgwm570, located on 6A, was closely linked to relative water content as well as grain protein content (R 2 = 16%) revealed by regression analysis. The correlation matrix displays a positive association between the control and stressed condition by R 2 = 0.92, 0.82, and 0.53 in malondialdehyde, relative water content, and grain protein, respectively. However, there was a negative correlation between water content–malondialdehyde and malondialdehyde–grain protein, though there was only a 4% correlation between grain protein content (control) and relative water content (stressed). Based on the tolerance matrix, WH730 and RAJ4079 were heat tolerant, and DBW173 and HD3086 were sensitive. Conclusions These findings indicate that to identify tolerant genotypes, physiological and biochemical traits can be utilized as an alternate criterion, and these closely associated markers can be applied for improved late-planted wheat production through MAS. The breeding scheme and genome editing by recognizing novel genes through physio-biochemical parameters, marker-assisted selection, and prospective screening of tolerant genotypes are proclaimed by the study.

Abstract Background Fusarium head blight (FHB), caused by Fusarium graminearum, is a major disease of wheat in North America. FHB infection causes fusarium damaged kernels (FDKs), accumulation of deoxynivalenol (DON) in the grain, and a reduction in quality and grain yield. Inheritance of FHB resistance is complex and involves multiple genes. The objective of this research was to identify QTL associated with native FHB and DON resistance in a ‘D8006W’/’Superior’, soft white winter wheat population. Results Phenotyping was conducted in replicated FHB field disease nurseries across multiple environments and included assessments of morphological and FHB related traits. Parental lines had moderate FHB resistance, however, the population showed transgressive segregation. A 1913.2 cM linkage map for the population was developed with SNP markers from the wheat 90 K Infinium iSelect SNP array. QTL analysis detected major FHB resistance QTL on chromosomes 2D, 4B, 5A, and 7A across multiple environments, with resistance from both parents. Trait specific unique QTL were detected on chromosomes 1A (visual traits), 5D (FDK), 6B (FDK and DON), and 7D (DON). The plant height and days to anthesis QTL on chromosome 2D coincided with Ppd-D1 and were linked with FHB traits. The plant height QTL on chromosome 4B was also linked with FHB traits; however, the Rht-B1 locus did not segregate in the population. Conclusions This study identified several QTL, including on chromosome 2D linked with Ppd-D1, for FHB resistance in a native winter wheat germplasm.

Abstract Background Septoria tritici blotch (STB) disease causes yield losses of up to 50 per cent in susceptible wheat cultivars and can reduce wheat production. In this study, genomic architecture for adult-plant STB resistance in a Septoria Association Mapping Panel (SAMP) having 181 accessions and genomic regions governing STB resistance in a South Asian wheat panel were looked for. Results Field experiments during the period from 2019 to 2021 revealed those certain accessions, namely BGD52 (CHIR7/ANB//CHIR1), BGD54 (CHIR7/ANB//CHIR1), IND92 (WH 1218), IND8 (DBW 168), and IND75 (PBW 800), exhibited a high level of resistance. Genetic analysis revealed the presence of 21 stable quantitative trait nucleotides (QTNs) associated with resistance to STB (Septoria tritici blotch) on all wheat chromosomes, except for 2D, 3A, 3D, 4A, 4D, 5D, 6B, 6D, and 7A. These QTNs were predominantly located in chromosome regions previously identified as associated with STB resistance. Three Quantitative Trait Loci (QTNs) were found to have significant phenotypic effects in field evaluations. These QTNs are Q.STB.5A.1, Q.STB.5B.1, and Q.STB.5B.3. Furthermore, it is possible that the QTNs located on chromosomes 1A (Q.STB.1A.1), 2A (Q.STB_DH.2A.1, Q.STB.2A.3), 2B (Q.STB.2B.4), 5A (Q.STB.5A.1, Q.STB.5A.2), and 7B (Q.STB.7B.2) could potentially be new genetic regions associated with resistance. Conclusion Our findings demonstrate the importance of Asian bread wheat as a source of STB resistance alleles and novel stable QTNs for wheat breeding programs aiming to develop long-lasting and wide-ranging resistance to Zymoseptoria tritici in wheat cultivars.

Abstract Crop statistics are crucial for developing a demand-based export and import strategy to ensure a country’s sustainable food security. Remote sensing efficiently generates essential crop statistics, while ground-based supplementary sensor data offers sufficient information for crop delineation. This study explored the multispectral satellite imagery using in-situ ground-based hyperspectral reflectance phenology information as training data to delineate wheat from other competitive winter crops in Northwestern Bangladesh as a case study. Wheat spectral signatures were primarily obtained through a hand-held Spectroradiometer at various phenological stages, aligned with Sentinel-2 data availability. Five vegetation indices (VIs), namely Normalized Difference Vegetation Index (NDVI), Red-edge NDVI (RENDVI), Enhanced Vegetation Index (EVI), Greenness Chromatic Coordinate (GCC) and Soil-Adjusted Vegetation Index (SAVI), were derived from Spectroradiometer-data across six wheat growth stages: seedling, tillering, booting, flowering, grain development, and maturity. Maximum and minimum threshold values for the VIs at those six growth stages were determined from regression analysis of the values collected from Spectroradiometer and Sentinel-2. A rule-based classification technique was then used to categorize Sentinel-2 for wheat crop delineation based on those threshold values. The results revealed that maps based on NDVI, EVI, and SAVI showed overall accuracies of 83.33%, 85.18%, and 81.48%, respectively. These accuracies were found to be statistically acceptable (p

Abstract Wheat is an important staple crop not only in Pakistan but all over the globe. Although the area dedicated to wheat cultivation expands annually, the quantity of wheat harvested is declining due to various biotic and abiotic factors. Global wheat production and output have suffered as a result of the drought, which is largely driven by a lack of water and environmental factors. Organic fertilizers have been shown to reduce the severity of drought. The current research was conducted in semi-arid climates to mitigate the negative effects of drought on wheat during its critical tillering (DTS), flowering (DFS), and grain filling (DGFS) stages through the application of three different abscisic acid treatments: ABA0 (0 mgL−1) control, ABA1 (100 mgL−1) and ABA2 (200 mgL−1). Wheat growth and yield characteristics were severely harmed by drought stress across all critical development stages, with the DGFS stage being particularly vulnerable and leading to a considerable loss in yield. Plant height was increased by 24.25%, the number of fertile tillers by 25.66%, spike length by 17.24%, the number of spikelets per spike by 16.68%, grain count per spike by 11.98%, thousand-grain weight by 14.34%, grain yield by 26.93% and biological yield by 14.55% when abscisic acid (ABA) was applied instead of the control treatment. Moreover, ABA2 increased the more physiological indices (water use efficiency (36.12%), stomatal conductance (44.23%), chlorophyll a (24.5%), chlorophyll b (29.8%), transpiration rate (23.03%), photosynthetic rate (24.84%), electrolyte leakage (− 38.76%) hydrogen peroxide (− 18.09%) superoxide dismutase (15.3%), catalase (20.8%), peroxidase (− 18.09%), and malondialdehyde (− 13.7%)) of drought-stressed wheat as compared to other treatments. In the case of N, P, and K contents in grain were maximally improved with the application of ABA2. Through the use of principal component analysis, we were able to correlate our results across scales and provide an explanation for the observed effects of ABA on wheat growth and production under arid conditions. Overall, ABA application at a rate of 200 mgL−1 is an effective technique to boost wheat grain output by mitigating the negative effects of drought stress.

Low intake of micro- and macroelements and vitamins in food negatively affects the health of more than two billion people around the world provoking chronic diseases. For the majority of the world’s population, these are soft and durum wheats that provide beneficial nutrients, however their modern high-yielding varieties have a significantly depleted grain mineral composition that have reduced mineral intake through food. Biofortification is a new research trend, whose main goal is to improve the nutritional qualities of agricultural crops using a set of classical (hybridization and selection) methods as well and the modern ones employing gene/QTL mapping, bioinformatic analysis, transgenesis, mutagenesis and genome editing. Using the classical breeding methods, biofortified varieties have been bred as a part of various international programs funded by HarvestPlus, CIMMYT, ICARDA. Despite the promise of transgenesis and genome editing, these labor-intensive methods require significant investments, so these technologies, when applied to wheat, are still at the development stage and cannot be applied routinely. In recent years, the interest in wheat biofortification has increased due to the advances in mapping genes and QTLs for agronomically important traits. The new markers obtained from wheat genome sequencing and application of bioinformatic methods (GWAS, meta-QTL analysis) has expanded our knowledge on the traits that determine the grain mineral concentration and has identified the key gene candidates. This review describes the current research on genetic biofortification of wheat in the world and in Russia and provides information on the use of cultivated and wild-relative germplasms to expand the genetic diversity of modern wheat varieties.

One of promising areas of wheat breeding is the creation of varieties with a high concentration of anthocyanins in the grain for the production of functional food products. Nonetheless, the question of how these compounds affect seed viability after long-term storage has remained unexplored. A comparative study on seed viability was conducted using a set of near-isogenic lines on the background of spring wheat variety Saratovskaya 29. These sister lines carry different combinations of recombinant DNA regions (on chromosomes 2A and 7D) containing dominant and recessive alleles at loci Pp3 and Pp-D1 (Pp: Purple pericarp), which determine the anthocyanin color of coleoptiles and of the pericarp. Seeds were germinated on two layers of water-moistened filter paper in a climatic chamber at a constant temperature of 20 °C on a 12-hour daylight cycle. During long-term natural storage of the seeds for up to 9 years in a dry ventilated room in Kraft bags at 20 ± 2 °C, the tested wheat samples experienced a loss of seed germination capacity of ~50 %; anthocyanins were found to not participate in the preservation of germination capacity. Nonetheless, anthocyanins contributed to the preservation of seed viability under unfavorable short-term conditions of a temperature rise to 48 °C at 100 % humidity. The accelerated aging test did not predict poor germination capacity after long-term seed storage. The results showed a neutral role of anthocyanins in the maintenance of seed germination capacity for 6–9 years under natural storage conditions at 20 ± 2 °C. A small statistically significant increase in grain germination capacity during natural aging was associated with the presence of a recombinant region containing the Pp-D1 gene on wheat chromosome 7D.

Low nutrient use efficiency (NUE) of conventional chemical fertilizers has resulted in the loss of costly nutrients and related environmental implications. Consequently, enhancing crop productivity and nutrient use efficiency are major challenges. In this backdrop, a field experiment was conducted to study the impact of pine oleoresin (POR) and neem oil (NO) coated urea (CU) fertilizers on crop productivity and nutrient recovery efficiency in maize-wheat cropping system grown on Vertisols of central India. The treatment combinations were POR-CU and NO-CU at 100% and 75% of recommended doses of fertilizers (RDF); normal urea (100% RDF); and an unfertilized control. Two years results indicated that the increment in grain yields due to POR-CU and NO-CU applications were 18.8% and 11.7% for maize and 11.6% and 3.49% for wheat, respectively, over normal urea. The apparent recovery efficiency of N (REN) for POR-CU, NO-CU, and normal urea at 100% RDF were 65.8%, 64.2%, and 51.4% in maize and 43.2%, 37.0%, and 34.6% in wheat, respectively. There was no significant difference noticed between POR-CU and NO-CU with respect to grain yield and N recovery efficiency. Hence, the study suggested that POR-CU could be a possible alternative option to NO-CU for improving crop yield and NUE. However, further research is needed to determine how effective POR-CU is in diverse agricultural systems and climatic conditions.

Societal Impact Statement Increasing crop productivity is often proposed as a key goal for meeting the food security demands of a growing global population. However, achieving high crop yields alone without meeting end‐use quality requirements is counter to this objective and can lead to negative environmental and sustainability issues. High yielding feed wheat crops in the United Kingdom are a typical example of this. The historical context of UK agricultural industrialisation, developments in plant breeding and wheat end‐use processing are examined. We then outline how employing innovations in plant breeding methods offer the potential to redress the balance between wheat quantity and quality. Summary Bread wheat (Triticum aestivum L.) has historically been an important crop for many human civilisations. Today, variability in wheat supply and trade has a large influence on global economies and food security. The United Kingdom is an example of an industrialised country that achieves high wheat yields through intensive cropping systems and a favourable climate. However, only a minority of the wheat grain produced is of suitable end‐use quality for modern bread baking methods and most wheat produced is fed to livestock. A large agricultural land area and input use dedicated to producing grain for animal rather than human food has wide‐ranging negative impacts for environmental sustainability and domestic food production. Here we present an historical perspective of agricultural and economic changes that have resulted in UK production primarily focussing on wheat quantity over quality. Agricultural intensification, liberalisation of free trade in agricultural commodities, innovations in the milling and baking sector, developments in scientific understanding of genetics and plant breeding, and geopolitical changes have all played a role. We propose that wheat breeding plays a crucial role in influencing these issues and although wheat breeders in the United Kingdom have historically applied the most‐up‐to‐date scientific advances, recent advances in genomics tools and quantitative genetics present a unique opportunity for breeders to redress the balance between quantity and quality.

Micronutrient deficiencies in agricultural soils significantly affect crop productivity and the nutritional quality of produce, posing potential risks to human health. Abandoned mine tailings, which are rich in micronutrients, can serve as an effective solution to enhance the nutritional value of crops while also mitigating environmental impact. In this study, the soils were sourced from the semi-arid Doukkala region in western Morocco, while the tailings were obtained from the abandoned Kettara mine in the nearby Marrakech region. This study assessed the biomass and bioconcentration of wheat (Triticum aestivum L.) grown in three different soils amended with treated mine tailings (TMT) at doses of 0, 0.2, 1, 2, and 4 g.kg-1. The experiment, conducted under greenhouse and in pots, employed a split-plot design with three replicates, monitoring morphological parameters and plant biomass. Concentrations of Cu, Fe, Zn, and Mn in wheat grains were measured by inductively coupled plasma-atomic emission spectrometry (ICP AES) after harvest. The results indicate an increase in root length, shoot height, number of tillers, shoot biomass and grain biomass with TMT amendment doses of less than 1 g.kg-1 soil, by 13.6, 42.1, 42.6, 49.6, and 32.9% respectively. However, these parameters decreased with doses >2 g.kg-1. Significant linear correlations were observed between the concentrations of micronutrients in wheat grains and those present in the soil. The bioconcentration factor increased but remained below 1. This research reveals that treated mine tailings (TMT) are perfectly suitable to fertilize wheat using doses

The purpose of the study is the determination of heavy metals in soil samples and their circulation in the food chain. In this study, the correlation between the presence of heavy metals in agricultural soils and the quality preservation of wheat, specifically Triticum Aestivum and Triticum Durum, was investigated. The results indicate that over 85% of the samples contain a high presence of metals compared to Kosovar standards. Land surveying was conducted at distances ranging from 20m to 400m from the highway. The results for Pb at a distance from 20 m showed a value of 2.11 mg·kg-1, while Zn varied with a value of 5.12 mg·kg-1. However, the amount of heavy metals in agricultural soil is not sufficient to determine their risk in the food chain. The samples studied from the wheat varieties exhibited the accumulation of metals in the wheat cultivated in the studied soils. Some wheat samples showed high levels of heavy metals, with Pb varying significantly with a value of 1.99 mg·kg-1, and Cr with a value of 6.12 mg·kg-1. In this study, the circulation of soil metals such as Pb, Cd, Cr, Fe, Cu, Zn, and Mn was monitored. The study then continued with the monitoring of soil physicochemical parameters as indicators of soil quality, such as organic carbon. The results showed variations depending on the distance and its concentration. The maximum protein value varied at 11.9%, gluten at 22.8%, moisture at 11.4%, etc., especially for Triticum E. and Triticum D. wheat varieties.

The development of high-yielding modern wheat varieties to feed the growing population has had a negative impact on the production of ancestral and landrace crops. The use of modern varieties, which are very deficient in vitamins, minerals, antioxidants, and flavonoids, has caused people to turn to old varieties due to health problems that arise over time. In this study, which aimed to determine the glutathione S-transferase (GST) enzyme activity of registered varieties and landraces, the differences between the protein values and GST enzyme activity values of wheat were found to be statistically significant. When protein values were analysed among wheat varieties, einkorn wheat had the highest value with 15.53 mg/ml, and KUNDURU-1149 had the second highest value with 13.52 mg/ml. The lowest protein values were found in wheat landraces. Lr-4 had the highest GST enzyme activity with 299.7 mmol/min/mg protein and Lr-10 with 265.3 mmol/min/mg protein. A negative and high correlation was found between wheat protein values and GST enzyme activity, and it was determined that landraces were prominent in terms of GST enzyme activity.

Abstract Heat stress poses a significant environmental challenge that profoundly impacts wheat productivity. It disrupts vital physiological processes such as photosynthesis, by impeding the functionality of the photosynthetic apparatus and compromising plasma membrane stability, thereby detrimentally affecting grain development in wheat. The scarcity of identified marker trait associations pertinent to thermotolerance presents a formidable obstacle in the development of marker-assisted selection strategies against heat stress. To address this, wheat accessions were systematically exposed to both normal and heat stress conditions and phenotypic data were collected on physiological traits including proline content, canopy temperature depression, cell membrane injury, photosynthetic rate, transpiration rate (at vegetative and reproductive stage and ‘stay-green’. Principal component analysis elucidated the most significant contributors being proline content, transpiration rate, and canopy temperature depression, which exhibited a synergistic relationship with grain yield. Remarkably, cluster analysis delineated the wheat accessions into four discrete groups based on physiological attributes. Moreover, to explore the relationship between physiological traits and DNA markers, 158 wheat accessions were genotyped with 186 SSRs. Allelic frequency and polymorphic information content value were found to be highest on genome A (4.94 and 0.688), chromosome 1A (5.00 and 0.712), and marker Xgwm44 (13.0 and 0.916). Population structure, principal coordinate analysis and cluster analysis also partitioned the wheat accessions into four subpopulations based on genotypic data, highlighting their genetic homogeneity. Population diversity and presence of linkage disequilibrium established the suitability of population for association mapping. Additionally, linkage disequilibrium decay was most pronounced within a 15–20 cM region on chromosome 1A. Association mapping revealed highly significant marker trait associations at Bonferroni correction P

Stem rust, caused by the biotrophic fungal pathogen Puccinia graminis f. sp. tritici (Pgt), is an important disease of wheat. However, the majority of Pgt virulence/avirulence loci and underlying genes remain uncharacterized due to the constraints of developing bi-parental populations with this obligate biotroph. Genome-wide association studies (GWAS) using a sexual Pgt population mainly collected from the Pacific Northwestern United States were used to identify candidate virulence/avirulence effector genes corresponding to the six wheat Sr genes: Sr5, Sr21, Sr8a, Sr17, Sr9a, and Sr9d. The Pgt isolates were genotyped using whole-genome shotgun sequencing that identified approximately 1.2 million single nucleotide polymorphisms (SNPs) and were phenotyped at the seedling stage on six Sr gene differential lines. Association mapping analyses identified 17 Pgt loci associated with virulence or avirulence phenotypes on six Pgt resistance genes. Among these loci, 16 interacted with a specific Sr gene, indicating Sr-gene specific interactions. However, one avirulence locus interacted with two separate Sr genes (Sr9a and Sr17), suggesting two distinct Sr genes identifying a single avirulence effector. A total of 24 unique effector gene candidates were identified, and haplotype analysis suggests that within this population, AvrSr5, AvrSr21, AvrSr8a, AvrSr17, and AvrSr9a are dominant avirulence genes, while avrSr9d is a dominant virulence gene. The putative effector genes will be fundamental for future effector gene cloning efforts, allowing for further understanding of rust effector biology and the mechanisms underlying virulence evolution in Pgt with respect to race-specific R-genes. [Figure: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Abstract Background Bread wheat (Triticum aestivum L.) endosperm contains starch and proteins, which determine the final yield, quality, and nutritional value of wheat grain. The preferentially expressed endosperm genes can precisely provide targets in the endosperm for improving wheat grain quality and nutrition using modern bioengineering technologies. However, the genes specifically expressed in developing endosperms remain largely unknown. Results In this study, 315 preferentially expressed endosperm genes (PEEGs) in the spring wheat landrace, Chinese Spring, were screened using data obtained from an open bioinformatics database, which reveals a unique grain reserve deposition process and special signal transduction in a developing wheat endosperm. Furthermore, transcription and accumulation of storage proteins in the wheat cultivar, XC26 were evaluated. The results revealed that 315 PEEG plays a critical role in storage protein fragment deposition and is a potential candidate for modifying grain quality and nutrition. Conclusion These results provide new insights into endosperm development and candidate genes and promoters for improving wheat grain quality through genetic engineering and plant breeding techniques.

Abstract Background Phytosulfokine (PSK) functions as a plant peptide growth factor that plays important and diverse roles in plant development and stress responses. Nevertheless, PSKs have not been systematically analyzed in wheat. Results A genome-wide comparative analysis of PSK genes in wheat was conducted and 15 TaPSKs were identified and divided into four subgroups in the wheat genome based on sequence similarity. The examination of motif compositions of TaPSK genes revealed the presence of the YIYTQ signature motif in the C-terminus of all TaPSK polypeptide precursors, with a highly conserved feature across different species. Exogenous application of TaPSK peptide promoted root growth in wheat. Quantitative RT-PCR analysis revealed that the TaPSKs exhibited preferential or tissue-specific expression patterns in wheat. In particular, three homologs of the TaPSK1 genes were specifically expressed in grains, with the strongest expression observed in the developing grains at 15 days after anthesis. Compared with wild type, transgenic rice lines overexpressing TaPSK1-A exhibited larger grain size and higher thousand-grain weight. The promoter region and genomic sequence of the wheat TaPSK1-A gene were cloned. Sequence polymorphism showed that five single-nucleotide polymorphisms (SNPs) were identified in the promoter region of TaPSK1-A. A Kompetitive Allele-Specific PCR (KASP) marker was developed for TaPSK1-A based on –806 bp SNP (C/T transition), and two haplotypes, TaPSK1-A-HapI and TaPSK1-A-HapII were detected in 260 wheat accessions collected from different regions. The expression of TaPSK1-A, promoter activity, and thousand-grain weight (TGW) in the TaPSK1-A-HapII haplotype were higher than those in the TaPSK1-A-HapI haplotype. Furthermore, yeast one-hybrid assays revealed the binding of TaNF-YB1 and TaERF39 to the promoter regions of the TaPSK1-A gene, and TaMADS29 could bind to the promoters of TaPSK1-B and TaPSK1-D genes. Conclusions Comparative genome-wide analysis of TaPSK peptide family revealed that the TaPSK1 gene is involved in wheat grain development, and the developed TaPSK1-A-KASP marker could be utilized for marker-assisted selection breeding of wheat. Graphical Abstract

Abstract Stripe rust, induced by Puccinia striiformis f. sp. tritici, is the most harmful and prevalent disease in temperate regions worldwide, affecting wheat production areas globally. An effective strategy for controlling the disease involves enhancing genetic resistance against stripe rust, achieved through Egyptian breeding efforts not previously conducted on wheat genotypes. The resistance level to stripe rust in thirty-eight wheat genotypes was assessed using marker-assisted selection methods. The investigation suggests that wheat breeding programs can utilize slow-rusting Yr genes, which are effective resistance genes, to develop novel genotypes with stripe rust resistance through marker-assisted breeding. Based on the four disease responses of the wheat genotypes under investigation, the results categorized the genotypes into three groups. The first group included resistant genotypes, the second group exhibited a slow-rusting character with the lowest disease symptom rates, and the last group displayed the highest disease characteristics rates throughout the three seasons, comprising fast-rusting genotypes. The rust-resistant genes identified were Yr5, Yr9, Yr10, Yr15, Yr17, Yr18, Yr26, Yr29, Yr30, and Yr36. Genes Yr26, Yr30, and Yr36 were present in all genotypes. Genotypes Misr3, Misr4, Giza168, Giza167, Giza170, Giza171, Gemmeiza9, and Gemmeiza10 carried the Yr9 gene. Only one genotype, Sids13, was found to have the Yr17 gene. Genes Yr18 and Yr29 were identified in Sids14, Giza168, Giza170, Gemmeiza9, and Gemmeiza10. However, none of the wheat genotypes showed the presence of Yr5, Yr10, or Yr15. Several backcrossing generations were conducted to introduce the Yr5 and Yr10 genes into susceptible genotypes (Misr1, Misr2, and Gemmeiza11). These genotypes are cultivated globally and are known for producing high-quality flour, making them of great importance to farmers. The study demonstrates significant potential for enhancing wheat genotypes for stripe rust resistance and increased production.

Abstract The pervasive existence of nanoplastics (NPs) and microplastics (MPs) in soil has become a worldwide environmental concern. N/MPs exist in the environment in a variety of forms, sizes, and concentrations, while multi-omics studies on the comprehensive impact of N/MPs with different properties (e.g. type and size) on plants remain limited. Therefore, this study utilized multi-omics analysis methods to investigate the effects of three common polymers [polyethylene-NPs (PE-NPs, 50 nm), PE-MPs (PE-MPs, 10 μm), and polystyrene-MPs (PS-MPs, 10 μm)] on the growth and stress response of wheat, as well as the rhizosphere microbial community at two concentrations (0.05 and 0.5 g/kg). PS and PE exhibited different effects for the same particle size and concentration. PE-NPs had the most severe stress effects, resulting in reduced rhizosphere bacteria diversity, plant biomass, and antioxidant enzyme activity while increasing beneficial bacteria richness. N/MPs altered the expression of nitrogen-, phosphorus-, and sulfur-related functional genes in rhizosphere bacteria, thereby affecting photosynthesis, as well as metabolite and gene levels in wheat leaves. Partial least squares pathway models (PLSPMs) indicated that concentration, size, and type play important roles in the impact of N/MPs on the plant ecological environment, which could have essential implications for assessing the environmental risk of N/MPs. Graphical Abstract

Abstract Seeds, considered as the foundation of agriculture, are invaded by a broad spectrum of seed-borne pathogens. The current study aimed to control seed-borne fungal pathogens of wheat, Aspergillus flavus and A. niger, by using Ag+ nanochitosan (Ag-NC) for nano-priming of seeds and enhancing seed quality. Nanochitosan (NC) and Ag-NC were synthesized using the gelation method and characterized by UV–vis spectrophotometry, FESEM, EDXS, and HRTEM. NC and Ag-NC showed irregular surface topography with an average particle size of 275 and 325 nm, respectively. Antifungal activity of both the nanoparticles at 0.1, 0.2, 0.3, 0.4, and 0.5 mg/mL revealed that Ag-NC at 0.5 mg/mL has completely terminated the mycelial growth of both pathogens. Malonaldehyde content increased to 77.77% in A. flavus and 82.66% in A. niger when exposed to 0.5 mg/mL Ag-NC. High-intensity fluorescence due to oxidative stress was observed in Ag-NC-treated pathogens. Ultra-structural changes in Ag-NC treated pathogenic spores under SEM displayed pronounced membrane damages. Wheat seeds were nano-primed with NC and Ag-NC at 0.5 mg/mL, and fungal load was examined to evaluate the mitigation of pathogenic stress and its effect on seedling growth promotion activity. Ag-NC priming reduced the fungal load and allowed successful seed germination. Ag-NC priming increased the albumin, gliadin, gluten, and glutenin content along with total phenol, reducing sugar and starch levels. Ag-NC priming increased the overall protein levels traced through SDS-PAGE. Seed priming with Ag-NC promotes seed germination, mean germination time, stress tolerance index, vigour, etc. NC and Ag-NC at 0.5 mg/mL showed no cytotoxic effect on the Human Embryonic Kidney (HEK293) cell line that ensures the nanoparticles are non-toxic. Thus, the synthesized nanoparticles exhibit a dual role in antifungal activity and plant growth promotion.

Abstract The fungus Parastagonospora nodorum causes septoria nodorum blotch on wheat. The role of the fungal Velvet-family transcription factor VeA in P. nodorum development and virulence was investigated here. Deletion of the P. nodorum VeA ortholog, PnVeA, resulted in growth abnormalities including pigmentation, abolished asexual sporulation and highly reduced virulence on wheat. Comparative RNA-Seq and RT-PCR analyses revealed that the deletion of PnVeA also decoupled the expression of major necrotrophic effector genes. In addition, the deletion of PnVeA resulted in an up-regulation of four predicted secondary metabolite (SM) gene clusters. Using liquid-chromatography mass-spectrometry, it was observed that one of the SM gene clusters led to an accumulation of the mycotoxin alternariol. PnVeA is essential for asexual sporulation, full virulence, secondary metabolism and necrotrophic effector regulation.

Abstract Background The male sterile lines are an important foundation for heterosis utilization in wheat (Triticum aestivum L.). Thereinto, pollen development is one of the indispensable processes of wheat reproductive development, and its fertility plays an important role in wheat heterosis utilization, and are usually influencing by genes. However, these key genes and their regulatory networks during pollen abortion are poorly understood in wheat. Results DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION 1 (TDF1) is a member of the R2R3-MYB family and has been shown to be essential for early tapetal layer development and pollen grain fertility in rice (Oryza sativa L.) and Arabidopsis thaliana. In order to clarify the function of TDF1 in wheat anthers development, we used OsTDF1 gene as a reference sequence and homologous cloned wheat TaTDF1 gene. TaTDF1 is localized in the nucleus. The average bolting time of Arabidopsis thaliana overexpressed strain (TaTDF1-OE) was 33 d, and its anther could be colored normally by Alexander staining solution, showing red. The dominant Mosaic suppression silence-line (TaTDF1-EAR) was blue-green in color, and the anthers were shrimpy and thin. The TaTDF1 interacting protein (TaMAP65) was confirmed using Yeast Two-Hybrid Assay (Y2H) and Bimolecular-Fluorescence Complementation (BiFC) experiments. The results showed that downregulated expression of TaTDF1 and TaMAP65 could cause anthers to be smaller and shrunken, leading to pollen abortion in TaTDF1 wheat plants induced by virus-induced gene-silencing technology. The expression pattern of TaTDF1 was influenced by TaMAP65. Conclusions Thus, systematically revealing the regulatory mechanism of wheat TaTDF1 during anther and pollen grain development may provide new information on the molecular mechanism of pollen abortion in wheat.

Abstract Background Septoria tritici blotch (STB), caused by the foliar fungus Zymoseptoria tritici, is one of the most damaging disease of wheat in Europe. Genetic resistance against this fungus relies on different types of resistance from non-host resistance (NHR) and host species specific resistance (HSSR) to host resistance mediated by quantitative trait loci (QTLs) or major resistance genes (Stb). Characterizing the diversity of theses resistances is of great importance for breeding wheat cultivars with efficient and durable resistance. While the functional mechanisms underlying these resistance types are not well understood, increasing piece of evidence suggest that fungus stomatal penetration and early establishment in the apoplast are both crucial for the outcome of some interactions between Z. tritici and plants. To validate and extend these previous observations, we conducted quantitative comparative phenotypical and cytological analyses of the infection process corresponding to 22 different interactions between plant species and Z. tritici isolates. These interactions included four major bread wheat Stb genes, four bread wheat accessions with contrasting quantitative resistance, two species resistant to Z. tritici isolates from bread wheat (HSSR) and four plant species resistant to all Z. tritici isolates (NHR). Results Infiltration of Z. tritici spores into plant leaves allowed the partial bypass of all bread wheat resistances and durum wheat resistance, but not resistances from other plants species. Quantitative comparative cytological analysis showed that in the non-grass plant Nicotiana benthamiana, Z. tritici was stopped before stomatal penetration. By contrast, in all resistant grass plants, Z. tritici was stopped, at least partly, during stomatal penetration. The intensity of this early plant control process varied depending on resistance types, quantitative resistances being the least effective. These analyses also demonstrated that Stb-mediated resistances, HSSR and NHR, but not quantitative resistances, relied on the strong growth inhibition of the few Z. tritici penetrating hyphae at their entry point in the sub-stomatal cavity. Conclusions In addition to furnishing a robust quantitative cytological assessment system, our study uncovered three stopping patterns of Z. tritici by plant resistances. Stomatal resistance was found important for most resistances to Z. tritici, independently of its type (Stb, HSSR, NHR). These results provided a basis for the functional analysis of wheat resistance to Z. tritici and its improvement.

The grain protein content (GPC) is the key parameter for wheat grain nutritional quality. This study conducted a resampling GWAS analysis using 406 wheat accessions across eight environments, and identified four previously reported GPC QTLs. An analysis of 87 landraces and 259 modern cultivars revealed the loss of superior GPC haplotypes, especially in Chinese cultivars. These haplotypes were preferentially adopted in different agroecological zones and had broad effects on wheat yield and agronomic traits. Most GPC QTLs did not significantly reduce yield, suggesting that high GPC can be achieved without a yield penalty. The results of this study provide a reference for future GPC breeding in wheat using the four identified QTLs.

Abstract Background Photo-thermo-sensitive male sterility (PTMS), which refers to the male sterility triggered by variations in photoperiod and temperature, is a crucial element in the wheat two-line hybrid system. The development of safe production and efficient propagation for male sterile lines holds utmost importance in two-line hybrid wheat. Under the stable photoperiod condition, PTMS is mainly induced by high or low temperatures in wheat, but the effect of daily temperature difference (DTD) on the fertility conversion of PTMS lines has not been reported. Here, three BS type PTMS lines including BS108, BS138, and BS366, as well as a control wheat variety J411 were used to analyze the correlation between fertility and DTD using differentially sowing tests, photo-thermo-control experiments, and transcriptome sequencing. Results The differentially sowing tests suggested that the optimal sowing time for safe seed production of the three PTMS lines was from October 5th to 25th in Dengzhou, China. Under the condition of 12 h 12 °C, the PTMS lines were greatly affected by DTD and exhibited complete male sterility at a temperature difference of 15 °C. Furthermore, under different temperature difference conditions, a total of 20,677 differentially expressed genes (DEGs) were obtained using RNA sequencing. Moreover, through weighted gene co-expression network analysis (WGCNA) and KEGG enrichment analysis, the identified DEGs had a close association with “starch and sucrose metabolism”, “phenylpropanoid biosynthesis”, “MAPK signaling pathway-plant”, “flavonoid biosynthesis”, and “cutin, and suberine and wax biosynthesis”. qRT-PCR analysis showed the expression levels of core genes related to KEGG pathways significantly decreased at a temperature difference of 15 ° C. Finally, we constructed a transcriptome mediated network of temperature difference affecting male sterility. Conclusions The findings provide important theoretical insights into the correlation between temperature difference and male sterility, providing guidance for the identification and selection of more secure and effective PTMS lines.

Small RNAs (sRNAs) are involved in gene silencing in multiple ways, including through cross-kingdom transfers from parasites to their hosts. Little is known about the evolutionary mechanisms enabling eukaryotic microbes to evolve functional mimics of host small regulatory RNAs. Here, we describe the identification and functional characterization of SINE_sRNA1, an sRNA family derived from highly abundant short interspersed nuclear element (SINE) retrotransposons in the genome of the wheat powdery mildew pathogen. SINE_sRNA1 is encoded by a sequence motif that is conserved in multiple SINE families and corresponds to a functional plant microRNA (miRNA) mimic targeting Tae_AP1, a wheat gene encoding an aspartic protease only found in monocots. Tae_AP1 has a novel function enhancing both pattern-triggered immunity (PTI) and effector-triggered immunity (ETI), thereby contributing to the cross activation of plant defenses. We conclude that SINE_sRNA1 and Tae_AP1 are functional innovations, suggesting the contribution of transposons to the evolutionary arms race between a parasite and its host. [Graphic: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Abstract The bacterial blight of wheat is an important global disease causing a significant decline in crop yield. Nanotechnology offers a potential solution for managing plant diseases. Therefore, this research aimed to investigate the effectiveness of silver nanoparticles (AgNPs) in controlling bacterial blight in 27 locally grown wheat cultivars. The study examined the impact of AgNPs at three distinct time points: 1, 3, and 5 days after the onset of the disease. Biochemical assay revealed that one day after applying the disease stress, the Inia cultivar had the highest amount of soluble protein (55.60 μg.g−1FW) content in the treatment without AgNPs. The Azadi cultivar, without AgNPs treatment, had the lowest amount of soluble protein content (15.71 μg.g−1FW). The Tabasi cultivar had the highest activity of the superoxide dismutase (SOD) (61.62 mM.g−1FW) with the combination treatment of AgNPs. On the other hand, the Karchia cultivar had the lowest SOD activity (0.6 mM.g−1FW) in the treatment of disease without AgNPs. Furthermore, three days after the application of stress, the Mahdavi cultivar had the highest amount of soluble protein content (54.16 μg.g−1FW) in the treatment of disease without AgNPs. The Niknejad cultivar had the highest activity of the SOD (74.15 mM.g−1FW) with the combined treatment of the disease without AgNPs. The Kavir cultivar had the lowest SOD activity (1.95 mM.g−1FW) and the lowest peroxidase (POX) activity (0.241 mM g−1FW min−1) in the treatment of the disease with AgNPs. Five days after exposure to stress, the Mahooti cultivar had the highest SOD activity (88.12 mM.g−1FW) with the combined treatment of the disease with AgNPs, and the Karchia cultivar had the lowest SOD activity (2.39 mM.g−1FW) in the treatment of the disease with AgNPs. Further, the results indicated that exposure to AgNPs could improve the antioxidant properties of wheat seeds in blight-infected and disease-free conditions in some cultivars.

Abstract Background Superoxide dismutase (SOD) can greatly scavenge reactive oxygen species (ROS) in plants. SOD activity is highly related to plant stress tolerance that can be improved by overexpression of SOD genes. Identification of SOD activity-related loci and potential candidate genes is essential for improvement of grain quality in wheat breeding. However, the loci and candidate genes for relating SOD in wheat grains are largely unknown. In the present study, grain SOD activities of 309 recombinant inbred lines (RILs) derived from the ‘Berkut’ × ‘Worrakatta’ cross were assayed by photoreduction method with nitro-blue tetrazolium (NBT) in four environments. Quantitative trait loci (QTL) of SOD activity were identified using inclusive composite interval mapping (ICIM) with the genotypic data of 50 K single nucleotide polymorphism (SNP) array. Results Six QTL for SOD activity were mapped on chromosomes 1BL, 4DS, 5AL (2), and 5DL (2), respectively, explaining 2.2 ~ 7.4% of the phenotypic variances. Moreover, QSOD.xjau-1BL, QSOD.xjau-4DS, QSOD.xjau-5 A.1, QSOD.xjau-5 A.2, and QSOD.xjau-5DL.2 identified are likely to be new loci for SOD activity. Four candidate genes TraesCS4D01G059500, TraesCS5A01G371600, TraesCS5D01G299900, TraesCS5D01G343100LC, were identified for QSOD.xjau-4DS, QSOD.xjau-5AL.1, and QSOD.xjau-5DL.1 (2), respectively, including three SOD genes and a gene associated with SOD activity. Based on genetic effect analysis, this can be used to identify desirable alleles and excellent allele variations in wheat cultivars. Conclusion These candidate genes are annotated for promoting SOD production and inhibiting the accumulation of ROS during plant growth. Therefore, lines with high SOD activity identified in this study may be preferred for future wheat breeding.

Abstract Background Squamosa promoter-binding protein-like (SPL) proteins are essential to plant growth and development as plant-specific transcription factors. However, the functions of SPL proteins in wheat need to be further explored. Results We cloned and characterized TaSPL6B of wheat in this study. Analysis of physicochemical properties revealed that it contained 961 amino acids and had a molecular weight of 105 kDa. Full-length TaSPL6B transcription activity was not validated in yeast and subcellular localization analysis revealed that TaSPL6B was distributed in the nucleus. Ectopic expression of TaSPL6B in Arabidopsis led to increasing number of branches and early flowering. TaSPL6B was highly transcribed in internodes of transgenic Arabidopsis. The expression of AtSMXL6/AtSMXL7/AtSMXL8 (homologous genes of TaD53) was markedly increased, whereas the expression of AtSPL2 (homologous genes of TaSPL3) and AtBRC1 (homologous genes of TaTB1) was markedly reduced in the internodes of transgenic Arabidopsis. Besides, TaSPL6B, TaSPL3 and TaD53 interacted with one another, as demonstrated by yeast two-hybrid and bimolecular fluorescence complementation assays. Therefore, we speculated that TaSPL6B brought together TaD53 and TaSPL3 and enhanced the inhibition effect of TaD53 on TaSPL3 through integrating light and strigolactone signaling pathways, followed by suppression of TaTB1, a key repressor of tillering. Conclusions As a whole, our findings contribute to a better understanding of how SPL genes work in wheat and will be useful for further research into how TaSPL6B affects yield-related traits in wheat.

Abstract Background Alfin-like proteins are a kind of plant-specific transcription factors, and play vital roles in plant growth, development and stress responses. Results In this study, a total of 27 Alfin-like transcription factors were identified in wheat. TaAL genes were unevenly distributed on chromosome. Phylogenetic analysis showed TaAL genes were divided into AL-B and AL-C subfamilies, and TaALs with closer evolutionary relationships generally shared more similar exon-intron structures and conserved motifs. The cis-acting element analysis showed MBS, ABRE and CGTCA-motif were the most common in TaAL promoters. The interacting proteins and downstream target genes of TaAL genes were also investigated in wheat. The transcriptome data and real-time PCR results indicated TaAL genes were differentially expressed under drought and salt stresses, and TaAL1-B was significantly up-regulated in response to drought stress. In addition, association analysis revealed that TaAL1-B-Hap-I allelic variation had significantly higher survival rate compared to TaAL1-B-Hap-II under drought stress. Conclusions These results will provide vital information to increase our understanding of the Alfin-like gene family in wheat, and help us in breeding better wheat varieties in the future.

Abstract Background Septoria tritici blotch (STB) is considered to be one of the most destructive foliar wheat diseases and is caused by Zymoseptoria tritici. The yield losses are severe and in Northwestern Europe can reach up to 50%. The efficacy of fungicides is diminishing due to changes in the genetic structure of the pathogen. Therefore, resistance breeding is the most effective strategy of disease management. Recently, genome-wide association studies (GWAS) have become more popular due to their robustness in dissecting complex traits, including STB resistance in wheat. This was made possible by the use of large mapping populations and new sequencing technologies. High-resolution mapping benefits from historical recombination and greater allele numbers in GWAS. Results In our study, 217 wheat genotypes of diverse origin were phenotyped against five Z. tritici isolates (IPO323, IPO88004, IPO92004, IPO86036 and St1-03) and genotyped on the DArTseq platform. In polytunnel tests two disease parameters were evaluated: the percentage of leaf area covered by necrotic lesions (NEC) and the percentage of leaf area covered by lesions bearing pycnidia (PYC). The disease escape parameters heading date (Hd) and plant height (Ht) were also measured. Pearson’s correlation showed a positive effect between disease parameters, providing additional information. The Structure analysis indicated four subpopulations which included from 28 (subpopulation 2) to 79 genotypes (subpopulation 3). All of the subpopulations showed a relatively high degree of admixture, which ranged from 60% of genotypes with less than 80% of proportions of the genome attributed to assigned subpopulation for group 2 to 85% for group 4. Haplotype-based GWAS analysis allowed us to identify 27 haploblocks (HBs) significantly associated with analysed traits with a p-value above the genome-wide significance threshold (5%, which was –log10(p) > 3.64) and spread across the wheat genome. The explained phenotypic variation of identified significant HBs ranged from 0.2% to 21.5%. The results of the analysis showed that four haplotypes (HTs) associated with disease parameters cause a reduction in the level of leaf coverage by necrosis and pycnidia, namely: Chr3A_HB98_HT2, Chr5B_HB47_HT1, Chr7B_HB36_HT1 and Chr5D_HB10_HT3. Conclusions GWAS analysis enabled us to identify four significant chromosomal regions associated with a reduction in STB disease parameters. The list of valuable HBs and wheat varieties possessing them provides promising material for further molecular analysis of resistance loci and development of breeding programmes.

Abstract Background Developing novel germplasm by using wheat wild related species is an effective way to rebuild the wheat resource bank. The Psathyrostachys huashanica Keng (P. huashanica, 2n = 2x = 14, NsNs) is regarded as a superior species to improve wheat breeding because of its multi-resistance, early maturation and numerous tiller traits. Introducing genetic components of P. huashanica into the common wheat background is the most important step in achieving the effective use. Therefore, the cytogenetic characterization and influence of the introgressed P. huashanica large segment chromosomes in the wheat background is necessary to be explored. Results In this study, we characterized a novel derived line, named D88-2a, a progeny of the former characterized wheat-P. huashanica partial amphiploid line H8911 (2n = 7x = 49, AABBDDNs). Cytological identification showed that the chromosomal composition of D88-2a was 2n = 44 = 22II, indicating the addition of exogenous chromosomes. Genomic in situ hybridization demonstrated that the supernumerary chromosomes were a pair of homologues from the P. huashanica and could be stably inherited in the common wheat background. Molecular markers and 15 K SNP array indicated that the additional chromosomes were derived from the sixth homoeologous group (i.e., 6Ns) of P. huashanica. Based on the distribution of the heterozygous single-nucleotide polymorphism sites and fluorescence in situ hybridization karyotype of each chromosome, this pair of additional chromosomes was confirmed as P. huashanica 6Ns large segment chromosomes, which contained the entire short arm and the proximal centromere portion of the long arm. In terms of the agronomic traits, the addition line D88-2a exhibited enhanced stripe rust resistance, improved spike characteristics and increased protein content than its wheat parent line 7182. Conclusions The new wheat germplasm D88-2a is a novel cytogenetically stable wheat-P. huashanica 6Ns large segment addition line, and the introgressed large segment alien chromosome has positive impact on plant spikelet number and stripe rust resistance. Thus, this germplasm can be used for genetic improvement of cultivated wheat and the study of functional alien chromosome segment.

Abstract Tilletia indica Mitra causes Karnal bunt (KB) in wheat by pathogenic dikaryophase. The present study is the first to provide the draft genomes of the dikaryon (PSWKBGD-3) and its two monosporidial lines (PSWKBGH-1 and 2) using Illumina and PacBio reads, their annotation and the comparative analyses among the three genomes by extracting polymorphic SSR markers. The trancriptome from infected wheat grains of the susceptible wheat cultivar WL711 at 24 h, 48h, and 7d after inoculation of PSWKBGH-1, 2 and PSWKBGD-3 were also isolated. Further, two transcriptome analyses were performed utilizing T. indica transcriptome to extract dikaryon genes responsible for pathogenesis, and wheat transcriptome to extract wheat genes affected by dikaryon involved in plant-pathogen interaction during progression of KB in wheat. A total of 54, 529, and 87 genes at 24hai, 48hai, and 7dai, respectively were upregulated in dikaryon stage while 21, 35, and 134 genes of T. indica at 24hai, 48hai, and 7dai, respectively, were activated only in dikaryon stage. While, a total of 23, 17, and 52 wheat genes at 24hai, 48hai, and 7dai, respectively were upregulated due to the presence of dikaryon stage only. The results obtained during this study have been compiled in a web resource called TiGeR ( http://backlin.cabgrid.res.in/tiger/ ), which is the first genomic resource for T. indica cataloguing genes, genomic and polymorphic SSRs of the three T. indica lines, wheat and T. indica DEGs as well as wheat genes affected by T. indica dikaryon along with the pathogenecity related proteins of T. indica dikaryon during incidence of KB at different time points. The present study would be helpful to understand the role of dikaryon in plant-pathogen interaction during progression of KB, which would be helpful to manage KB in wheat, and to develop KB-resistant wheat varieties.

Abstract Background Biofortification represents a promising and sustainable strategy for mitigating global nutrient deficiencies. However, its successful implementation poses significant challenges. Among staple crops, wheat emerges as a prime candidate to address these nutritional gaps. Wheat biofortification offers a robust approach to enhance wheat cultivars by elevating the micronutrient levels in grains, addressing one of the most crucial global concerns in the present era. Main text Biofortification is a promising, but complex avenue, with numerous limitations and challenges to face. Notably, micronutrients such as iron (Fe), zinc (Zn), selenium (Se), and copper (Cu) can significantly impact human health. Improving Fe, Zn, Se, and Cu contents in wheat could be therefore relevant to combat malnutrition. In this review, particular emphasis has been placed on understanding the extent of genetic variability of micronutrients in diverse Triticum species, along with their associated mechanisms of uptake, translocation, accumulation and different classical to advanced approaches for wheat biofortification. Conclusions By delving into micronutrient variability in Triticum species and their associated mechanisms, this review underscores the potential for targeted wheat biofortification. By integrating various approaches, from conventional breeding to modern biotechnological interventions, the path is paved towards enhancing the nutritional value of this vital crop, promising a brighter and healthier future for global food security and human well-being.

Abstract To sustainably increase wheat yield to meet the growing world population’s food demand in the face of climate change, Conservation Agriculture (CA) is a promising approach. Still, there is a lack of genomic studies investigating the genetic basis of crop adaptation to CA. To dissect the genetic architecture of 19 morpho-physiological traits that could be involved in the enhanced adaptation and performance of genotypes under CA, we performed GWAS to identify MTAs under four contrasting production regimes viz., conventional tillage timely sown (CTTS), conservation agriculture timely sown (CATS), conventional tillage late sown (CTLS) and conservation agriculture late sown (CALS) using an association panel of 183 advanced wheat breeding lines along with 5 checks. Traits like Phi2 (Quantum yield of photosystem II; CATS:0.37, CALS: 0.31), RC (Relative chlorophyll content; CATS:55.51, CALS: 54.47) and PS1 (Active photosystem I centers; CATS:2.45, CALS: 2.23) have higher mean values in CA compared to CT under both sowing times. GWAS identified 80 MTAs for the studied traits across four production environments. The phenotypic variation explained (PVE) by these QTNs ranged from 2.15 to 40.22%. Gene annotation provided highly informative SNPs associated with Phi2, NPQ (Quantum yield of non-photochemical quenching), PS1, and RC which were linked with genes that play crucial roles in the physiological adaptation under both CA and CT. A highly significant SNP AX94651261 (9.43% PVE) was identified to be associated with Phi2, while two SNP markers AX94730536 (30.90% PVE) and AX94683305 (16.99% PVE) were associated with NPQ. Identified QTNs upon validation can be used in marker-assisted breeding programs to develop CA adaptive genotypes.

Abstract Background The number of spikelets per spike is a key trait that affects the yield of bread wheat (Triticum aestivum L.). Identification of the QTL for spikelets per spike and its genetic effects that could be used in molecular assistant breeding in the future. Results In this study, four recombinant inbred line (RIL) populations were generated and used, having YuPi branching wheat (YP), with Supernumerary Spikelets (SS) phenotype, as a common parent. QTL (QSS.sicau-2 A and QSS.sicau-2D) related to SS trait were mapped on chromosomes 2 A and 2D through bulked segregant exome sequencing (BSE-Seq). Fourteen molecular markers were further developed within the localization interval, and QSS.sicau-2 A was narrowed to 3.0 cM covering 7.6 Mb physical region of the reference genome, explaining 13.7 − 15.9% the phenotypic variance. Similarly, the QSS.sicau-2D was narrowed to 1.8 cM covering 2.4 Mb physical region of the reference genome, and it explained 27.4 − 32.9% the phenotypic variance. These two QTL were validated in three different genetic backgrounds using the linked markers. QSS.sicau-2 A was identified as WFZP-A, and QSS.sicau-2D was identified a novel locus, different to the previously identified WFZP-D. Based on the gene expression patterns, gene annotation and sequence analysis, TraesCS2D03G0260700 was predicted to be a potential candidate gene for QSS.sicau-2D. Conclusion Two significant QTL for SS, namely QSS.sicau-2 A and QSS.sicau-2D were identified in multiple environments were identified and their effect in diverse genetic populations was assessed. QSS.sicau-2D is a novel QTL associated with the SS trait, with TraesCS2D03G0260700 predicted as its candidate gene.

Abstract To obtain seasonable and precise crop yield information with fine resolution is very important for ensuring the food security. However, the quantity and quality of available images and the selection of prediction variables often limit the performance of yield prediction. In our study, the synthesized images of Landsat and MODIS were used to provide remote sensing (RS) variables, which can fill the missing values of Landsat images well and cover the study area completely. The deep learning (DL) was used to combine different vegetation index (VI) with climate data to build wheat yield prediction model in Hebei Province (HB). The results showed that kernel NDVI (kNDVI) and near-infrared reflectance (NIRv) slightly outperform normalized difference vegetation index (NDVI) in yield prediction. And the regression algorithm had a more prominent effect on yield prediction, while the yield prediction model using Long Short-Term Memory (LSTM) outperformed the yield prediction model using Light Gradient Boosting Machine (LGBM). The model combining LSTM algorithm and NIRv had the best prediction effect and relatively stable performance in single year. The optimal model was then used to generate 30 m resolution wheat yield maps in the past 20 years, with higher overall accuracy. In addition, we can define the optimum prediction time at April, which can consider simultaneously the performance and lead time. In general, we expect that this prediction model can provide important information to understand and ensure food security.

Genomic selection is a technology that allows for the determination of the genetic value of varieties of agricultural plants and animal breeds, based on information about genotypes and phenotypes. The measured breeding value (BV) for varieties and breeds in relation to the target trait allows breeding stages to be thoroughly planned and the parent forms suitable for crossing to be chosen. In this work, the BLUP method was used to assess the breeding value of 149 Russian varieties and introgression lines (4 measurements for each variety or line, 596 phenotypic points) of spring wheat according to the content of seven chemical elements in the grain – K, Ca, Mg, Mn, Fe, Zn, Cu. The quality of the evaluation of breeding values was assessed using cross-validation, when the sample was randomly divided into five parts, one of which was chosen as a test population. The following average values of the Pearson correlation were obtained for predicting the concentration of trace elements: K – 0.67, Ca – 0.61, Mg – 0.4, Mn – 0.5, Fe – 0.38, Zn – 0.46, Cu – 0.48. Out of the 35 models studied, the p-value was below the nominal significant threshold (p-value < 0.05) for 28 models. For 11 models, the p-value was significant after correction for multiple testing (p-value < 0.001). For Ca and K, four out of five models and for Mn two out of five models had a p-value below the threshold adjusted for multiple testing. For 30 varieties that showed the best varietal values for Ca, K and Mn, the average breeding value was 296.43, 785.11 and 4.87 mg/kg higher, respectively, than the average breeding value of the population. The results obtained show the relevance of the application of genomic selection models even in such limited-size samples. The models for K, Ca and Mn are suitable for assessing the breeding value of Russian wheat varieties based on these characteristics.

Abstract Protein phosphorylation plays an important role in immune signaling transduction in plant resistance to pathogens. Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), severely devastates wheat production. Nonetheless, the molecular mechanism of wheat resistance to stripe rust remains limited. In this study, quantitative phosphoproteomics was employed to investigate the protein phosphorylation changes in wheat challenged by Pst. A total of 1537 and 2470 differentially accumulated phosphoproteins (DAPs) were identified from four early infection stage (6, 12, 18 and 24 h post-inoculation) in incompatible and compatible wheat-Pst interactions respectively. KEGG analysis revealed that Oxidative Phosphorylation, Phosphatidylinositol Signaling, and MAPK signaling processes are distinctively enriched in incompatible interaction, while Biosynthesis of secondary metabolites and RNA degradation process were significantly enriched in compatible interactions. In particular, abundant changes in phosphorylation levels of chloroplast proteins were identified, suggesting the regulatory role of photosynthesis in wheat-Pst interaction, which is further emphasized by protein-protein interaction (PPI) network analysis. Motif-x analysis identified [xxxxSPxxxx] motif, likely phosphorylation sites for defensive response-related kinases, and a new [xxxxSSxxxx] motif significantly enriched in incompatible interaction. The results shed light on the early phosphorylation events contributing to wheat resistance against Pst. Moreover, our study demonstrated that the phosphorylation levels of Nucleoside diphosphate kinase TaNAPK1 are upregulated at 12 hpi with CYR23 and at 24 hpi with CYR31. Transient silencing of TaNAPK1 was able to attenuate wheat resistance to CYR23 and CYR31. Our study provides new insights into the mechanisms underlying Pst-wheat interactions and may provide database to find potential targets for the development of new resistant varieties.

Abstract Background The massive structural variations and frequent introgression highly contribute to the genetic diversity of wheat, while the huge and complex genome of polyploid wheat hinders efficient genotyping of abundant varieties towards accurate identification, management, and exploitation of germplasm resources. Results We develop a novel workflow that identifies 1240 high-quality large copy number variation blocks (CNVb) in wheat at the pan-genome level, demonstrating that CNVb can serve as an ideal DNA fingerprinting marker for discriminating massive varieties, with the accuracy validated by PCR assay. We then construct a digitalized genotyping CNVb map across 1599 global wheat accessions. Key CNVb markers are linked with trait-associated introgressions, such as the 1RS·1BL translocation and 2NvS translocation, and the beneficial alleles, such as the end-use quality allele Glu-D1d (Dx5 + Dy10) and the semi-dwarf r-e-z allele. Furthermore, we demonstrate that these tagged CNVb markers promote a stable and cost-effective strategy for evaluating wheat germplasm resources with ultra-low-coverage sequencing data, competing with SNP array for applications such as evaluating new varieties, efficient management of collections in gene banks, and describing wheat germplasm resources in a digitalized manner. We also develop a user-friendly interactive platform, WheatCNVb ( http://wheat.cau.edu.cn/WheatCNVb/ ), for exploring the CNVb profiles over ever-increasing wheat accessions, and also propose a QR-code-like representation of individual digital CNVb fingerprint. This platform also allows uploading new CNVb profiles for comparison with stored varieties. Conclusions The CNVb-based approach provides a low-cost and high-throughput genotyping strategy for enabling digitalized wheat germplasm management and modern breeding with precise and practical decision-making.

Abstract Wheat is a cereal crop that contributes to food security; thus, Ethiopia must boost the production efficiency of wheat to meet the sustainable development goal of eradicating hunger and poverty. Consequently, a significant revolution is occurring in the Ethiopian wheat industry to improve production and productivity. Therefore, it is critical to understand the current level of wheat farmers’ efficiency, as its production is highly influenced by existing agricultural technologies and climate change, which makes it dynamic. Accordingly, this study employed the parametric Cobb–Douglas stochastic frontier and two-limit Tobit models to evaluate wheat farmers’ efficiency and determine their drivers in Ethiopia’s largest wheat-producing area, the Arsi Zone. A multistage sampling strategy was applied to obtain a representative sample of 422 wheat farmers. The model’s output suggested that the average technical, allocative, and economic efficiency scores were 80.8%, 88.1%, and 71.3%, respectively. It is confirmed that wheat farmers’ efficiencies can increase with household head age, education level, livestock ownership, contact with extension agents, wheat mechanization, and involvement in non/off-farm activities but decrease with household distance from the main market and total land holdings. To realize the potential gains from wheat cultivation in Ethiopia, the government needs to develop policies and strategies that enhance farmers’ education, livestock production, and extension contact, facilitate infrastructure, market development, and wheat mechanization, and promote non/off-farm activities.

At the major vernalization locus ‘Vrn-D1’, the spring allele, Vrn-D1a, and two winter alleles, Vrn-D1b and vrn-D1, have been identified in wheat. We conducted field trials to determine the effects of alleles on growth and yield-related traits, using near-isogenic lines developed in the genetic background of spring type wheat cultivars, ‘Abukumawase’ and ‘Asakazekomugi’, both of which carry the Vrn-D1a allele. Although maturity date of three alleles was same, the panicle development occurred earlier in the following order of genotype: Vrn-D1a > Vrn-D1b > vrn-D1. There was a significant difference in panicle development even between two winter type alleles vrn-D1 and Vrn-D1b. Grain yield and the spikelet number per spike of the original spring type cultivars were lower than those of the winter type NILs. In addition, while the young panicle development of the tillers in the spring type cultivars was found to be two weeks to one month later in the tillers than that in the main stem, young panicle development of the main stem and tillers in the winter type NILs were almost identical. Hence, winter type wheat bearing vrn-D1 which exhibit later panicle development than that bearing Vrn-D1b, was elucidated to might be more useful especially in areas with high risk of frost damage. Spring type wheat had a lower sink capacity and grain yield, meanwhile that was considered to prevent severe frost damage by allowing late-developed tillers to survive.

In this study, 19 gluten protein traits of 44 wheat varieties were analyzed. Principal component analysis (PCA), correlation analysis (CA), membership function (MF) and stepwise regression (SR) were used for comprehensive evaluation of wheat gluten protein. The results showed that the variations of disulfide bond content ranged from 3.28 to 6.78 μmol/g, and the variations of sulfhydryl group content ranged from 13.30 to 16.66 μmol/g. The variation range of gluten protein traits was 4.74% to 89.46%, with larger variation coefficients being observed for stabilization time and farinograph quality index, indicating huge variation potential for protein quality traits. Three comprehensive factors were converted from the 19 protein traits by PCA, with contribution rates of 40.80%, 19.86% and 15.88%, respectively, and a cumulative contribution rate of 76.54%. PCA classified gluten proteins into three categories including gluten quality traits, aggregation traits and quantitative traits. The comprehensive evaluation value (D-value) of gluten was calculated using the MF method, and the average of the 12 traits with higher PCA loadings were found to be significantly correlated with D-values. Regression equations with seven traits including disulfide bond content, glutenin macropolymer (GMP) content, protein content, dry gluten content, gluten index, tensile area and peak maximum time (PMT) as independent variables were established using SR. There was a good linear relationship between bread quality indexes and D-value, with determination coefficient (R2) between 0.561 4 and 0.771 3, which verified the accuracy and feasibility of D-value. It could be concluded that wheat gluten protein with different gluten strengths exhibited abundant variation potential. It is feasible to comprehensively evaluate gluten protein quality by multivariate statistical analysis. The quantity and quality of gluten protein as well as the content of disulfide bonds in the gluten network could be used as key indexes for the evaluation and improvement of wheat quality.

Under the pressures of global climate change and human activities, the carrying capacity of water and soil resources in agricultural lands has decreased, and the traditional models of agricultural development are no longer sustainable. Land degradation, groundwater quality reduction and ecosystem instability are the consequences of agricultural development without considering sustainability indicators. This article aims to investigate the use of variable relationship analysis and Bayesian network methods to analyze and investigate the relationship between irrigation in agriculture and the sustainability of the groundwater ecosystem. Descriptive statistics of agriculture including cultivation pattern, time, precipitation, irrigation, and land slope were analyzed and combined with the simulated characteristics of groundwater including specific yield, hydraulic conductivity and hydrodynamic diffusion coefficients. Five crops of wheat, barley, paddy, alfalfa, and potato were studied to evaluate the effect of plants on the pattern of nitrate release due to irrigation and fertilization in agriculture. The results showed that managing the amount of fertilizer and the volume of irrigation can positively affect the nitrate distribution pattern in the groundwater even in a short period of time. HIGHLIGHTS The relationships between variables are used to build a logical model for analysis.; The quantity and quality of irrigation water is incorporated into the system for estimating the nitrate concentration in groundwater.;

Salinity is a significant environmental factor that prevents plant germination and growth, thus affecting agricultural productivity globally. Plant growth regulators, such as 6-benzylaminopurine, gibberellin, and melatonin, have been studied for their ability to mitigate the adverse effects of salt stress on wheat, a salt-tolerant staple crop cultivated worldwide. A field experiment was conducted before planting, involving three wheat varieties (Al-Ezz, Dijlah, and Al-Rasheed) subjected to different seed treatment combinations (salt tempering as control, salt tempering + benzyl stimulation, salt tempering + gibberellin stimulation, and salt tempering + melatonin stimulation), all irrigated with saline well water (6 ds m-2) except for the control group irrigated with river water. The findings highlighted the superior performance of the Al-Rasheed variety, exhibiting the highest levels of chlorophyll, relative water content, peroxidase enzyme, and catalase activity (1.993 mg g-1, 83.51%, 40.09 units ml-1, 34.54 units ml-1, respectively). The combination of salt-adaptation treatment and benzyl stimulation resulted in the highest chlorophyll content (2.117 mg g-1), while salt-adaptation treatment and melatonin stimulation showed the highest peroxidase and catalase activity (47.46 units ml-1, 42.71 units ml-1), along with increased grain yield (58.34 g) and harvest index (48.43%). Melatonin and benzylaminopurine have demonstrated potential in alleviating salt stress in plants by influencing various physiological traits.

A field experiment on loamy soil was conducted in the Haditha district, Anbar province, Iraq, during the winter season of 2021-2022. The study aimed to determine the effect of different zinc levels and methods on wheat growth, yield, and tolerance to salinity. The experiment used a randomized complete block design (RCBD) with a split-plot arrangement and three replications. The seeds were soaked before planting with three zinc levels: 0 (control treatment, soaked in water only), 75 mg Zn Kg-1 (seeds soaked in zinc at a concentration of 75 mg Zn Kg-1), and 150 mg Zn Kg-1 (seeds soaked in zinc at a concentration of 150 mg Zn Kg-1) for eight hours. This was followed by two spray applications of the same concentrations, the first at the tillering stage and the second at the booting stage. Statistical analysis showed that adding zinc to soak the seeds and spraying it on the plants increased plant height, number of tillers, flag leaf area, grain yield, and 1000-grain weight. Additionally, it was observed that seed soaking and plant spraying with zinc increased the plant's tolerance to salinity by reducing the chloride and sodium concentration in the wheat straw and roots and increasing the potassium concentration and the potassium/sodium ratio in the wheat straw and roots. The best results were obtained using the 150 mg Zn Kg-1 level (seed soaking and plant spraying) for most of the studied characteristics.

A laboratory experiment was carried out under the Seed Technology Laboratory conditions, Field Crops Department, College of Agriculture, Anbar University, Iraq, during the 2021 season to study the effect of coating with yeast extract at different concentrations (0, 15, 20, and 25 g L-1) on germination, seedling characteristics, and enzymatic activity under heat stress (5, 10, 15, and 20 °C) of wheat seeds of the Ibaa-99 cultivar. The experiment was conducted using a factorial design in a completely randomized design (CRD) with four repetitions. The obtained results showed a significant response of wheat seeds to the yeast extract treatments, with the 25 g L-1 yeast extract coating treatment showing superiority in germination speed (59.75%), final germination percentage (69.25%), radicle and plumule lengths (9.453 cm, 8.429 cm), seedling vigor index (1662.5), and enzyme activity (10.394 for catalase and 53.46 for peroxidase). Regarding cultivation under heat stress conditions, the temperature of 20 °C was significantly better for germination speed (67.25%), final germination percentage (87.62%), radicle and plumule lengths (10.703 cm and 9.551 cm), seedling vigor index (1812.3), and enzyme activity (catalase and peroxidase: 13.474 and 56.2). The interaction between coating seeds with 25 g yeast extract L-1 and growing at 20 °C achieved the highest values for germination speed, final germination percentage, radicle and plumule lengths, and seedling vigor index, while the same coating treatment at 10 °C recorded the highest activity of catalase and peroxidase enzymes.

A field experiment was carried out during the winter season of 2021-2022 in the Nuaimiya area of Fallujah City, Anbar Governorate, to study the effect of three potassium levels on the growth and yield of the wheat crop. The experiment was conducted using a randomized complete block design (RCBD) according to the arrangement of the split-plot with three replicates. The main plots included three varieties of wheat (Ibaa 99, Al-Rasheed, and Abu Ghraib-3), while the sub-plots included three levels of potassium: 0, 100, and 200 kg K ha-1. The results showed the superiority of the Al-Rasheed variety in each of the following: flag leaf area (46.13 cm²), spike length (14.8 cm), number of spikes per square meter (416.1 spikes m-2), number of grains per spike (52.33 grains spike-1), and total grain yield (4.963 tons ha-1). In contrast, the Ibaa 99 variety gave the highest average plant height of 121.11 cm. The Abu Ghraib-3 variety recorded the highest average weight of 1000 grains at 47.8 g. Potassium fertilizer levels significantly affected most growth and yield traits. The 200 kg K ha-1 level gave the highest means for plant height (119.63 cm), flag leaf area (41.51 cm²), spike length (13.65 cm), number of spikes per square meter (417.0 spikes m-2), number of grains per spike (55.22 grains spike-1), and total grain yield (5.004 tons ha-1). There was a significant interaction between the Ibaa 99 variety and the 200 kg K ha-1 level of potassium fertilizer in plant height, and between the Al-Rasheed variety and the same level in flag leaf area, spike length, number of spikes per square meter, and number of grains per spike.

Understanding stability for grain yield in combination with the physiological traits would make screening for spot blotch successful in wheat, as this is presently required under the changing climatic condition. The present study was conducted with 37 wheat genotypes from Indian Institute of Wheat & Barley Research (ICAR-IIWBR, Karnal) including two checks (Sonalika and Raj 3765) under timely sown and late sown conditions over two years (2017-18 and 2018-19) at Instructional Farm of Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar, West Bengal, India. The wheat genotypes differed significantly for all the seven morpho-reproductive traits. The G×E interaction was found to be significant for all the traits. In different traits different genotypes were found to be adaptable to favourable and unfavourable environments, created by the different dates of sowing over the two years. Regarding stability for grain yield the genotypes NEST-17-04 and NEST-17-37 were found to be promising for optimum yield over varying environments.

The present study was carried out to evaluate variability among 50 diverse genotypes of wheat (Triticum aestivum L.) for yield attributes and biotic factor like spot blotch during 2018-19 rabi season. Observations were recorded for morpho-phenetic traits, physiological parameter and disease observations of spot blotch (Bipolaris sorokiniana (Sacc.) Shoem) was done at four crop growth stages viz. 85 DAS, 92 DAS, 99 DAS and 106 DAS. The mean value of chlorophyll index (CI) indicated gradual decline of CI with advancement in growth stages. Highest value of AUCIDC was shown by ESWYT-50 (128.33), while the lowest value was exhibited by ESWYT-25 (22.75). Mean value of disease severity in the different growth stages indicated gradual increase in disease severity along with advancement in growth stages. Correlation study among AUDPC, AUCIDC along with three morpho-phenotypic traits revealed significant and positive correlation between AUDPC and AUCIDC. The D2 analysis distributed 50 wheat genotypes into six clusters. The genotypes having lower selection index values were ESWYT-36, ESWYT-40, ESWYT-22, ESWYT-35, ESWYT-18, ESWYT-10, and ESWYT-1, which were also distributed in different divergent clusters as per D2 analysis. Thus, they could be used as spot blotch resistant genotypes with early flowering type along with high yield. All the traits exhibited low PCV and GCV values indicating the presence of common parents in the ancestry of the wheat genotypes.