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Background: Brassica napus L. (B. napus) is susceptible to waterlogging stress during different cultivation periods. Therefore, it is crucial to enhance the resistance to waterlogging stress to achieve a high and stable yield of B. napus. Results: Here we observed significant differences in the responses of two B. napus varieties in root under waterlogging stress. The sensitive variety (23651) exhibited a more pronounced and rapid reduction in cell wall thickness and root integrity compared with the tolerant variety (Santana) under waterlogging stress. By module clustering analysis based on transcriptome data, we identified that cell wall polysaccharide metabolism responded to waterlogging stress in root. It was found that pectin content was significantly reduced in the sensitive variety compared with the tolerant variety. Furthermore, transcriptome analysis revealed that the expression of two homologous genes encoding polygalacturonase-inhibiting protein 2 (PGIP2), involved in polysaccharide metabolic pathways, was highly upregulated in root of the tolerant variety under waterlogging stress. BnaPGIP2s probably confer waterlogging resistance by inhibiting the activity of polygalacturonases (PGs), which in turn reduces the degradation of the pectin backbone polygalacturonic acid. Conclusions: Our findings demonstrate that cell wall polysaccharides in root plays a vital role in response to the waterlogging stress and provide a theoretical foundation for breeding waterlogging resistance in B. napus varieties. [ABSTRACT FROM AUTHOR]

Understanding the mechanisms that regulate plant root growth under soil drying is an important challenge in root biology. We observed that moderate soil drying promotes wheat root growth. To understand whether metabolic and hormonic changes are involved in this regulation, we performed transcriptome sequencing on wheat roots under well-watered and moderate soil drying conditions. The genes upregulated in wheat roots under soil drying were mainly involved in starch and sucrose metabolism and benzoxazinoid biosynthesis. Various plant hormone-related genes were differentially expressed during soil drying. Quantification of the plant hormones under these conditions showed that the concentrations of abscisic acid (ABA), cis-zeatin (CZ), and indole-3-acetic acid (IAA) significantly increased during soil drying, whereas the concentrations of salicylic (SA), jasmonic (JA), and glycosylated salicylic (SAG) acids significantly decreased. Correlation analysis of total root length and phytohormones indicated that CZ, ABA, and IAA are positively associated with wheat root length. These results suggest that changes in metabolic pathways and plant hormones caused by moderate soil drying help wheat roots grow into deeper soil layers. [ABSTRACT FROM AUTHOR]

We consider two spectral problems on an equilateral rooted tree with the standard (continuity and Kirchhoff's type) conditions at the interior vertices (except of the root if it is interior) and Dirichlet conditions at the pendant vertices (except of the root if it is pendant). For the first (Neumann) problem we impose the standard conditions (if the root is an interior vertex) or Neumann condition (if the root is a pendant vertex) at the root, while for the second (Dirichlet) problem we impose the Dirichlet condition at the root. We show that for caterpillar trees the spectra of the Neumann problem and of the Dirichlet problem uniquely determine the shape of the tree. Also, we present an example of co-spectral snowflake graphs. [ABSTRACT FROM AUTHOR]

For a nilpotent group G without π -torsion, and x , y ∈ G , if x n = y n for a π -number n , then x = y ; if x m y n = y n x m for π -numbers m , n , then x y = y x. This is a well-known result in group theory. In this paper, we prove two analogous theorems on matrices, which have independence significance. Specifically, let m be a given positive integer and A a complex square matrix satisfying that (i) all eigenvalues of A are nonnegative, and (ii) rank ⁡ A 2 = rank ⁡ A ; then A has a unique m -th root X with rank ⁡ X 2 = rank ⁡ X , all eigenvalues of X are nonnegative, and moreover there is a polynomial f (λ) with X = f (A). In addition, let A and B be complex n × n matrices with all eigenvalues nonnegative, and rank ⁡ A 2 = rank ⁡ A , rank ⁡ B 2 = rank ⁡ B ; then (i) A = B when A r = B r for some positive integer r , and (ii) A B = B A when A s B t = B t A s for two positive integers s and t. [ABSTRACT FROM AUTHOR]

Litter and root decomposition is an important source of soil organic matter and nutrients. To ascertain the contribution of litter and root to natural grassland nutrients in rocky desertification areas, from March 2017 to January 2018, the continuous soil column method, collector method, and litter decomposition method were used to study the soil nutrients, litter and root biomass, decomposition, and nutrient release of potential, moderate, and severe rocky desertification grasslands, as well as their responses to rocky desertification. The results showed that the litter and root decomposition rate showed a trend of being first fast and then slow, and the decomposition rate of litter and root was greater than 50% after 300 days. The annual litter decomposition rates of potential, moderate, and severe rocky desertification grasslands were 69.98%, 62.14%, and 49.79%, respectively, and the annual decomposition rates of root were 73.64%, 67.61%, and 64.09%, respectively. With a deepening degree of rocky desertification, the litter and root decomposition rate decreased. The decomposition coefficients, k, of litter in potential, moderate, and severe rocky desertification grasslands were 1.128, 0.896, and 0.668, respectively, and the decomposition coefficients, k, of root were 1.152, 1.018, and 0.987, respectively. The nutrient release processes of litter and root were different, and the release mode ultimately manifests as "release". In rocky desertification grasslands, the organic carbon (OC), total nitrogen (TN), total phosphorus (TP), and total potassium (TK) released by litter and root decomposition were 18.93–263.03 g·m−2·yr−1, 1.79–5.59 g·m−2·yr−1, 0.18–0.47 g·m−2·yr−1, and 0.66–3.70 g·m−2·yr−1, respectively. The contribution of root to soil nutrients was greater than that of litter. The degree of rocky desertification was negatively correlated with the biomass, decomposition rate, and nutrient return amount of litter and root. The results of this study provide direct field evidence and illustrate the contribution of litter and root decomposition in rocky desertification grasslands to soil nutrients. [ABSTRACT FROM AUTHOR]

This article explores the impact of salinity on two different maize cultivars and their ability to tolerate salt stress. The study reveals that the more salt-tolerant cultivar is better at excluding sodium (Na+) and retaining potassium (K+), which are crucial for minimizing Na+ uptake. The researchers also discovered that the salt-tolerant cultivar experienced a net influx of hydrogen ions (H+) under severe salt stress, while the salt-sensitive cultivar did not. This suggests that net H+ influx is an important but often overlooked trait in salt tolerance. Additionally, the study found that K+ efflux and Na+ influx help maintain charge balance during H+ influx. [Extracted from the article]

Nitrogen (N) is essential for sugar beet (Beta vulgaris L.), a highly N-demanding sugar crop. This study investigated the morphological, subcellular, and microRNA-regulated responses of sugar beet roots to low N (LN) stress (0.5 mmol/L N) to better understand the N perception, uptake, and utilization in this species. The results showed that LN led to decreased dry weight of roots, N accumulation, and N dry matter production efficiency, along with damage to cell walls and membranes and a reduction in organelle numbers (particularly mitochondria). Meanwhile, there was an increase in root length (7.2%) and branch numbers (29.2%) and a decrease in root surface area (6.14%) and root volume (6.23%) in sugar beet after 7 d of LN exposure compared to the control (5 mmol/L N). Transcriptomics analysis was confirmed by qRT-PCR for 6 randomly selected microRNAs, and we identified 22 differentially expressed microRNAs (DEMs) in beet root under LN treatment. They were primarily enriched in functions related to binding (1125), ion binding (641), intracellular (437) and intracellular parts (428), and organelles (350) and associated with starch and sucrose metabolism, tyrosine metabolism, pyrimidine metabolism, amino sugar and nucleotide sugar metabolism, and isoquinoline alkaloid biosynthesis, as indicated by the GO and KEGG analyses. Among them, the upregulated miR156a, with conserved sequences, was identified as a key DEM that potentially targets and regulates squamosa promoter-binding-like proteins (SPLs, 104889216 and 104897537) through the microRNA-mRNA network. Overexpression of miR156a (MIR) promoted root growth in transgenic Arabidopsis, increasing the length, surface area, and volume. In contrast, silencing miR156a (STTM) had the opposite effect. Notably, the fresh root weight decreased by 45.6% in STTM lines, while it increased by 27.4% in MIR lines, compared to the wild type (WT). It can be inferred that microRNAs, especially miR156, play crucial roles in sugar beet root's development and acclimation to LN conditions. They likely facilitate active responses to N deficiency through network regulation, enabling beet roots to take up nutrients from the environment and sustain their vital life processes. [ABSTRACT FROM AUTHOR]

Plant leaves are a source of essential phenolic compounds, which have numerous health benefits and can be used in multiple applications. While various techniques are available for recovering bioactive compounds from by-products, more data are needed on enzyme-assisted extraction (EAE). The aim of this study was to compare EAE and solid–liquid extraction (SLE), to evaluate the impact on bioactive compounds' extraction yield, phytochemical composition, and the antioxidant, antimicrobial, and antidiabetic properties of Aralia cordata leaves and roots, sea buckthorn Hippophae rhamnoides, and hemp Cannabis sativa leaves. The results indicate that EAE with Viscozyme L enzyme (EAE_Visc) extracts of the tested plant leaves possess the highest yield, antioxidant activity, and total phenolic content. Moreover, the EAE_Visc extract increased by 40% the total sugar content compared to the control extract of A. cordata root. Interestingly, the sea buckthorn leaf extracts exhibited α-glucosidase inhibitory activity, which reached an almost 99% inhibition in all extracts. Furthermore, the sea buckthorn leaves SLE and EAE_Visc extracts possess antibacterial activity against Staphylococcus aureus. Additionally, scanning electron microscopy was used to examine changes in cell wall morphology after EAE. Overall, this study shows that EAE can be a promising method for increasing the yield and improving the functional properties of the resulting extracts in a fast and sustainable way compared to SLE. [ABSTRACT FROM AUTHOR]

The bottle gourd [Lagenaria siceraria (Molina) Standl.] is often utilized as a rootstock for watermelon grafting. This practice effectively mitigates the challenges associated with continuous cropping obstacles in watermelon cultivation. The lower ground temperature has a direct impact on the rootstocks' root development and nutrient absorption, ultimately leading to slower growth and even the onset of yellowing. However, the mechanisms underlying the bottle gourd's regulation of root growth in response to low root zone temperature (LRT) remain elusive. Understanding the dynamic response of bottle gourd roots to LRT stress is crucial for advancing research regarding its tolerance to low temperatures. In this study, we compared the physiological traits of bottle gourd roots under control and LRT treatments; root sample transcriptomic profiles were monitored after 0 h, 48 h and 72 h of LRT treatment. LRT stress increased the malondialdehyde (MDA) content, relative electrolyte permeability and reactive oxygen species (ROS) levels, especially H2O2 and O2−. Concurrently, LRT treatment enhanced the activities of antioxidant enzymes like superoxide dismutase (SOD) and peroxidase (POD). RNA-Seq analysis revealed the presence of 2507 and 1326 differentially expressed genes (DEGs) after 48 h and 72 h of LRT treatment, respectively. Notably, 174 and 271 transcription factors (TFs) were identified as DEGs compared to the 0 h control. We utilized quantitative real-time polymerase chain reaction (qRT-PCR) to confirm the expression patterns of DEGs belonging to the WRKY, NAC, bHLH, AP2/ERF and MYB families. Collectively, our study provides a robust foundation for the functional characterization of LRT-responsive TFs in bottle gourd roots. Furthermore, these insights may contribute to the enhancement in cold tolerance in bottle gourd-type rootstocks, thereby advancing molecular breeding efforts. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Soil & Water Conservation (1009-2242) is the property of Institute of Soil & Water Conservation and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: The plant root system is critical for the absorption of water and nutrients, and have a direct influence on growth and yield. In cucumber, a globally consumed crop, the molecular mechanism of root development remains unclear, and this has implications for developing stress tolerant varieties. This study sought to determine the genetic patterns and related genes of cucumber root weight. A core cucumber germplasms population was used to do the GWAS analysis in three environments. Results: Here, we investigated four root-weight related traits including root fresh weight (RFW), root dry weight (RDW), ratio of root dry weight to root fresh weight (RDFW) and the comprehensive evaluation index, D-value of root weight (DRW) deduced based on the above three traits for the core germplasm of the cucumber global repository. According to the D-value, we identified 21 and 16 accessions with light and heavy-root, respectively. We also found that the East Asian ecotype accessions had significantly heavier root than other three ecotypes. The genome-wide association study (GWAS) for these four traits reveals that 4 of 10 significant loci (gDRW3.1, gDRW3.2, gDRW4.1 and gDRW5.1) were repeatedly detected for at least two traits. Further haplotype and expression analysis for protein-coding genes positioned within these 4 loci between light and heavy-root accessions predicted five candidate genes (i.e., Csa3G132020 and Csa3G132520 both encoding F-box protein PP2-B1 for gDRW3.1, Csa3G629240 encoding a B-cell receptor-associated protein for gDRW3.2, Csa4G499330 encodes a GTP binding protein for gDRW4.1, and Csa5G286040 encodes a proteinase inhibitor for gDRW5.1). Conclusions: We conducted a systematic analysis of the root genetic basis and characteristics of cucumber core germplasms population. We detected four novel loci, which regulate the root weight in cucumber. Our study provides valuable candidate genes and haplotypes for the improvement of root system in cucumber breeding. [ABSTRACT FROM AUTHOR]

Background: Studies have shown that plant endophytic microbial communities are ubiquitous and closely related to plant growth and health. To clarify the mechanism of the melon varieties with high resistant to wilt, the endophytic microbial compositions and metabolites in roots of melon varieties with high resistant ability to wilt were analyzed. Results: The results showed that the abundances of Firmicutes, Ascomycota, Bacillus, Bradyrhizobium, Amycolatopsis, Actinospica, and Catenulispora all increased in roots of wilt high resistant melon varieties (MT) which compared to wilt susceptible melon varieties (MS). Meanwhile, Ochrobactrum, Bordetella, Roseateles, Staphylococcus, Acidovorax, Amycolatopsis, Catenulispora, Promicromonospora, and Gymnopilus were the unique endophytic microbes in roots of MT. Moreover, in comparison with the MS varieties, the functions of Defense mechanisms, Secondary metabolites biosynthesis, transport and catabolism, Nucleotide transport and metabolism, Signal transduction mechanisms, Coenzyme transport and metabolism, Carbohydrate transport and metabolism and Amino acid transport and metabolism all increased in roots of MT varieties. Additionally, the nucleotide metabolism and biosynthesis of cofactors metabolic pathways were also significantly increased in roots of MT varieties. On the other hand, the untargeted metabolome results showed that Biosynthesis of various plant secondary metabolites, Nucleotide metabolism and Biosynthesis of cofactors metabolic pathways were significantly increased in the expression of MT varieties; and the content of metabolic compounds such as flavonoids, Cinnamic acid compounds, Organic acid compounds, and Nucleotides were increased. In addition, the correlation between microbiome and metabolome indicates a significant correlation between the two. Conclusions: All above results suggested that higher abundant antagonistic microbes and metabolic functions of endophytes in roots of wilt high resistant melon varieties (MT) were the important mechanisms for their high resistance to wilt. [ABSTRACT FROM AUTHOR]

Background: Understanding the root canal morphology is essential for the success of root canal treatment. Therefore, this study aimed to evaluate and analyze the root canal configuration of maxillary premolars using Cone Beam Computed Tomography in the Pakistani subpopulation. Method: This cross-sectional study utilized CBCT scans from two distinct centres: Aga Khan University in Karachi and Jinnah MRI and Body Scans in Lahore. The CBCT images were visualized using GALAXIS version 1.9 (SICAT GmbH and Co. KG, Bonn, Germany), integrated within the Sirona Dental System (D-64625 Bensheim, Germany). The scanning parameters were standardized at 85 kV, 7 mA, with a 15-s exposure time and a voxel size of 0.16 mm. A total of 707 CBCT scans were collected, encompassing 2180 maxillary premolars. Root canal configurations were classified based on (Ahmed et al. Int Endod J. 2017;50(8):761–70). Statistical analyses were performed using SPSS version 26, employing the Chi-square test with a significance level set at p < 0.05. Results: The distribution of root canal morphologies varied significantly with age and gender. Among maxillary premolars, 50% exhibited the typical configuration of 2MPMB1 L1 (two roots, single canal in each buccal and lingual root), while 26% of maxillary right second premolars displayed 1MPM1 (one root, one canal). Overall, 1MPM1 accounted for 27.4% of the total cases in the second premolars. There was no statistically significant relationship between age and root canal distribution in either first premolars (p = 0.338) or second premolars (p = 0.833). Regarding gender, a significant difference was observed in the distribution of right maxillary 1st premolars (p = 0.022*), with a higher prevalence among females. Conclusion: This study offers significant insights into the anatomical variations of root canals in maxillary premolars across diverse regional subpopulations in Pakistan. While specific root canal configurations were prevalent, the findings indicate no statistically significant correlation between age and root canal morphology in maxillary premolars. However, a notable gender disparity was observed in the distribution of the right maxillary first premolars. [ABSTRACT FROM AUTHOR]

The yield of sweet potato [Ipomoea batatas (L.) Lam] can be easily threatened by drought stress. Typically, early stages like the seedling stage and tuber-root expansion stage are more vulnerable to drought stress. In this study, a highly drought-tolerant sweet potato cultivar "WanSu 63" was subjected to drought stress at both the seedling stage (15 days after transplanting, 15 DAT) and the tuber-root expansion stage (45 DAT). Twenty-four cDNA libraries were constructed from leaf segments and root tissues at 15 and 45 DAT for Next-Generation Sequencing. A total of 663, 063, and 218 clean reads were obtained and then aligned to the reference genome with a total mapped ratio greater than 82.73%. A sum of 7119, 8811, 5463, and 930 differentially expressed genes were identified from leaves in 15 days (L15), roots in 15 days (R15), leaves in 45 days (L45), and roots in 45 days (R45), respectively, in drought stress versus control. It was found that genes encoding heat shock proteins, sporamin, LEA protein dehydrin, ABA signaling pathway protein gene NCED1, as well as a group of receptor-like protein kinases genes were enriched in differentially expressed genes. ABA content was significantly higher in drought-treated tissues than in the control. The sweet potato biomass declined sharply to nearly one-quarter after drought stress. In conclusion, this study is the first to identify the differentially expressed drought-responsive genes and signaling pathways in the leaves and roots of sweet potato at the seedling and root expansion stages. The results provide potential resources for drought resistance breeding of sweet potato. [ABSTRACT FROM AUTHOR]

Objectives: Oro‐dental manifestations of hyperoxaluria and dental management of affected patients are rarely reported in the literature. We describe a new oral presentation of primary hyperoxaluria (PH) and review relevant literature about oro‐dental manifestations and management of dental complications of hyperoxaluria. Methods: A case report of a 44‐year‐old female who presented with symptoms of temporomandibular joint dysfunction due to hyperoxaluria was described according to the CARE guidelines. In addition, an extensive search of biomedical databases (PubMed, Medline, Google Scholar, and Embase) for articles describing oro‐dental manifestations and/or dental management in patients with hyperoxaluria was performed using the key words ("oral" and/or "hyperoxaluria" and/or "dental" and/or "oxalosis"). Included articles were reviewed and data about patient demographics, disease type and stage, oral and dental manifestations, and dental treatment outcome were retrieved and analyzed. Results: A total of 14 articles describing the oral and dental manifestations in 15 patients with hyperoxaluria were included. Tooth mobility, root resorption, and radiographic alterations were consistently described in all cases. Oral manifestations were described mainly in PH at late stages, and only after the onset of chronic renal disease. Dental management in all reported cases was palliative and aimed to relive pain and treat periodontal infection. Tooth loss due to extraction or uncontrolled mobility was the ultimate outcome in almost all reported cases. Conclusion: Oral and dental manifestations in hyperoxaluria are rarely reported in the literature. Management of tooth mobility and root resorption in hyperoxaluria is challenging and clinical guidelines and evidence‐based recommendations are lacking. Early diagnosis and treatment of hyperoxaluria might be the only effective approach to prevent dental and periodontal complications of the disease. [ABSTRACT FROM AUTHOR]

Quinoa, a dicotyledonous annual herb belonging to the subfamily Amaranthaceae, has remarkable resistance to cold, drought, and barren conditions. However, few studies have reported on quinoa root metabolism under waterlogging stress. In this study, quinoa strains were treated with waterlogging, and the control group was exposed to normal water management conditions; waterlog-resistant (Dianli-188) and waterlog-sensitive (Dianli-60) strains were selected. Ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was used to analyze the root systems of four groups of quinoa seedlings (Dianli-60 treatment group: TR4; Dianli-60 control group: CR4; Dianli-188 treatment group: TR5; Dianli-188 control group: CR5). In total, 1044 metabolites were detected. Kyoto Encyclopedia of Genes and Genomes analysis was used to identify 648 differential metabolites involved in biosynthesis. In addition, we found that alanine, aspartate, and glutamate metabolism and glycolysis, tricarboxylic cycle, and butanoate metabolism pathways were related to the synthesis of γ-aminobutyric acid (GABA). The metabolites of C5-branched dibasic acid metabolism, urea cycle, starch and sucrose metabolism, phenolic metabolism, and flavonoid biosynthesis pathways were found to participate in the waterlogging stress response. Sucrose, glucose, and trehalose provide sufficient energy for adventitious root production. GABA regulates phenylpropane metabolism, including regulation of phenolic acids and flavonoids to enhance the antioxidant capacity of quinoa in response to waterlogging stress. This study provided a theoretical basis for breeding quinoa waterlog-resistant varieties, we can screen waterlogging-resistant strains based on the content of these metabolites in future. Thereby accelerating the breeding process of quinoa. [ABSTRACT FROM AUTHOR]

Due to the high demands of data communication, the broadcasting system streams the data daily. This service not only sends out the message to the correct participant but also respects the security of the identity user. In addition, when delivered, all the information must be protected for the party who employs the broadcasting service. Currently, Attribute-Based Broadcast Encryption (ABBE) is useful to apply for the broadcasting service. (ABBE) is a combination of Attribute-Based Encryption (ABE) and Broadcast Encryption (BE), which allows a broadcaster (or encrypter) to broadcast an encrypted message, including a predefined user set and specified access policy to install the authorization mechanism. It is desirable to hide all the information when producing in the ciphertext, which has not been considered in the previous works of ABBE. Motivated by the above issue, we devise a solution to achieve anonymity for the ABBE scheme, which not only hides the access structures but also anonymizes the user's identity. In this work, we propose two schemes as Anonymous Key Policy (AKP)-ABBE and Anonymous Ciphertext Policy (ACP)-ABBE with supporting multiple access structures by using OR / AND gates. Specifically, we present the generic constructions of AKP/ACP-ABBE on the building block of the Inner Product Encryption (IPE ), which enables the hidden user's identity and complex OR / AND -Gate access structure. We show that our proposed schemes are secured under the standard models. [ABSTRACT FROM AUTHOR]

Understanding how plant nutrient acquisition strategies respond to grazing at the community level is critical understanding ecosystem structure and functioning in grasslands. However, few studies have simultaneously compared the difference in above‐ground (leaf) and below‐ground (root) nutrient‐acquisition strategies in response to long‐term grazing, especially at the regional scale.Here, we measured a set of leaf and fine‐root traits that correspond to the fast‐slow economic spectrum at the community level in 10 experimental sites from paired grazed and ungrazed grasslands across a soil nutrient gradient covering three major types of grasslands in northern China.We found that patterns of variations of leaf and fine‐root traits were consistent with both a leaf and root economic spectrum at the community level for both grazed and non‐grazed plots. Grazing had minor effect on community‐level leaf nutrient‐acquisition strategies but strongly influenced community‐level root nutrient‐acquisition strategies. Specifically, root nutrient‐acquisition strategies were shifted to more exploitative resource use in grazed communities. Moreover, soil nutrient contributed to the changes in both leaf and root nutrient‐acquisition strategies, which tended towards a more resource acquisition strategy with increasing soil nutrient levels. Grazing significantly interacted with soil nutrient to affect root nutrient‐acquisition strategies, and grazing contributed more to root nutrient‐acquisition strategies than soil nutrient.Synthesis. Our results demonstrated completely inconsistent responses of community‐level above‐ and below‐ground resource acquisition strategies to long‐term grazing, and below‐ground acquisition strategies were more sensitive to long‐term grazing. Our findings also suggest that high intensity anthropogenic activities such as grazing may strongly modify below‐ground resource acquisition strategies. Read the free Plain Language Summary for this article on the Journal blog. [ABSTRACT FROM AUTHOR]

Aims: Our understanding of the rhizosphere is limited by the lack of techniques for in situ live microscopy. Current techniques are either destructive or unsuitable for observing chemical changes within the pore space. To address this limitation, we have developed artificial substrates, termed smart soils, that enable the acquisition and 3D reconstruction of chemical sensors attached to soil particles. Methods: The transparency of smart soils was achieved using polymer particles with refractive index matching that of water. The surface of the particles was modified both to retain water and act as a local sensor to report on pore space pH via fluorescence emissions. Multispectral signals were acquired from the particles using a light sheet microscope, and machine learning algorithms predicted the changes and spatial distribution in pH at the surface of the smart soil particles. Results: The technique was able to predict pH live and in situ within ± 0.5 units of the true pH value. pH distribution could be reconstructed across a volume of several cubic centimetres around plant roots at 10 μm resolution. Using smart soils of different composition, we revealed how root exudation and pore structure create variability in chemical properties. Conclusion: Smart soils captured the pH gradients forming around a growing plant root. Future developments of the technology could include the fine tuning of soil physicochemical properties, the addition of chemical sensors and improved data processing. Hence, this technology could play a critical role in advancing our understanding of complex rhizosphere processes. [ABSTRACT FROM AUTHOR]

A network of 15 Surface Elevation Tables (SETs) at North Inlet estuary, South Carolina, has been monitored on annual or monthly time scales beginning from 1990 to 1996 and continuing through 2022. Of 73 time series in control plots, 12 had elevation gains equal to or exceeding the local rate of sea-level rise (SLR, 0.34 cm/year). Rising marsh elevation in North Inlet is dominated by organic production and, we hypothesize, is proportional to net ecosystem production. The rate of elevation gain was 0.47 cm/year in plots experimentally fertilized for 10 years with N&P compared to nearby control plots that have gained 0.1 cm/year in 26 years. The excess gains and losses of elevation in fertilized plots were accounted for by changes in belowground biomass and turnover. This is supported by bioassay experiments in marsh organs where at age 2 the belowground biomass of fertilized S. alterniflora plants was increasing by 1,994 g m−2 year−1, which added a growth premium of 2.4 cm/year to elevation gain. This was contrasted with the net belowground growth of 746 g m−2 year−1 in controls, which can add 0.89 cm/year to elevation. Root biomass density was greater in the fertilized bioassay treatments than in controls, plateauing at about 1,374 g m−2 and 472 g m−2, respectively. Growth of belowground biomass was dominated by rhizomes, which grew to 3,648 g m−2 in the fertilized treatments after 3 years and 1,439 g m−2 in the control treatments after 5 years. Depositional wetlands are limited by an exogenous supply of mineral sediment, whereas marshes like North Inlet could be classified as autonomous because they depend on in situ organic production to maintain elevation. Autonomous wetlands are more vulnerable to SLR because their elevation gains are constrained ultimately by photosynthetic efficiency. [ABSTRACT FROM AUTHOR]

Summary: Barley (Hordeum vulgare) is an important global cereal crop and a model in genetic studies. Despite advances in characterising barley genomic resources, few mutant studies have identified genes controlling root architecture and anatomy, which plays a critical role in capturing soil resources.Our phenotypic screening of a TILLING mutant collection identified line TM5992 exhibiting a short‐root phenotype compared with wild‐type (WT) Morex background. Outcrossing TM5992 with barley variety Proctor and subsequent SNP array‐based bulk segregant analysis, fine mapped the mutation to a cM scale. Exome sequencing pinpointed a mutation in the candidate gene HvPIN1a, further confirming this by analysing independent mutant alleles.Detailed analysis of root growth and anatomy in Hvpin1a mutant alleles exhibited a slower growth rate, shorter apical meristem and striking vascular patterning defects compared to WT. Expression and mutant analyses of PIN1 members in the closely related cereal brachypodium (Brachypodium distachyon) revealed that BdPIN1a and BdPIN1b were redundantly expressed in root vascular tissues but only Bdpin1a mutant allele displayed root vascular defects similar to Hvpin1a.We conclude that barley PIN1 genes have sub‐functionalised in cereals, compared to Arabidopsis (Arabidopsis thaliana), where PIN1a sequences control root vascular patterning. [ABSTRACT FROM AUTHOR]

Background: Soybean establishes a mutualistic interaction with nitrogen-fixing rhizobacteria, acquiring most of its nitrogen requirements through symbiotic nitrogen fixation. This crop is susceptible to water deficit; evidence suggests that its nodulation status—whether it is nodulated or not—can influence how it responds to water deficit. The translational control step of gene expression has proven relevant in plants subjected to water deficit. Results: Here, we analyzed soybean roots' differential responses to water deficit at transcriptional, translational, and mixed (transcriptional + translational) levels. Thus, the transcriptome and translatome of four combined-treated soybean roots were analyzed. We found hormone metabolism-related genes among the differentially expressed genes (DEGs) at the translatome level in nodulated and water-restricted plants. Also, weighted gene co-expression network analysis followed by differential expression analysis identified gene modules associated with nodulation and water deficit conditions. Protein-protein interaction network analysis was performed for subsets of mixed DEGs of the modules associated with the plant responses to nodulation, water deficit, or their combination. Conclusions: Our research reveals that the stand-out processes and pathways in the before-mentioned plant responses partially differ; terms related to glutathione metabolism and hormone signal transduction (2 C protein phosphatases) were associated with the response to water deficit, terms related to transmembrane transport, response to abscisic acid, pigment metabolic process were associated with the response to nodulation plus water deficit. Still, two processes were common: galactose metabolism and branched-chain amino acid catabolism. A comprehensive analysis of these processes could lead to identifying new sources of tolerance to drought in soybean. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Prevention & Treatment For Stomatological Diseases is the property of Journal of Prevention & Treatment For Stomatological Diseases Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

SUMMARY: Plants partly optimize their water recruitment from the growth medium by directing root growth toward a moisture source, a phenomenon termed hydrotropism. The default mechanism of downward growth, termed gravitropism, often functions to counteract hydrotropism when the water‐potential gradient deviates from the gravity vector. This review addresses the identity of the root sites in which hydrotropism‐regulating factors function to attenuate gravitropism and the interplay between these various factors. In this context, the function of hormones, including auxin, abscisic acid, and cytokinins, as well as secondary messengers, calcium ions, and reactive oxygen species in the conflict between these two opposing tropisms is discussed. We have assembled the available data on the effects of various chemicals and genetic backgrounds on both gravitropism and hydrotropism, to provide an up‐to‐date perspective on the interactions that dictate the orientation of root tip growth. We specify the relevant open questions for future research. Broadening our understanding of root mechanisms of water recruitment holds great potential for providing advanced approaches and technologies that can improve crop plant performance under less‐than‐optimal conditions, in light of predicted frequent and prolonged drought periods due to global climate change. Significance Statement: Understanding the mechanisms that plant roots utilize to resist the strong tendency to grow downward in order to grow toward a moisture source, holds great potential for unveiling research approaches and technologies to improve crop performance. Herein, we assess the complex interplay between major regulators of root growth, development, and responses to myriad internal and external cues, that function to attenuate downward root growth, thereby enabling the root to reach water. [ABSTRACT FROM AUTHOR]

Proso millet (Panicum miliaceum L.) is resilient to abiotic stress, especially to drought. However, the mechanisms by which its roots adapt and tolerate salt stress are obscure. In this study, to clarify the molecular mechanism of proso millet in response to drought stress, the physiological indexes and transcriptome in the root of seedlings of the proso millet cultivar 'Yumi 2' were analyzed at 0, 0.5, 1.0, 1.5, and 3.0 h of stimulated drought stress by using 20% PEG-6000 and after 24 h of rehydration. The results showed that the SOD activity, POD activity, soluble protein content, MDA, and O2−· content of 'Yumi 2' increased with the time of drought stress, but rapidly decreased after rehydration. Here, 130.46 Gb of clean data from 18 samples were obtained, and the Q30 value of each sample exceeded 92%. Compared with 0 h, the number of differentially expressed genes (DEGs) reached the maximum of 16,105 after 3 h of drought, including 9153 upregulated DEGs and 6952 downregulated DEGs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that upregulated DEGs were mainly involved in ATP binding, nucleus, protein serine/threonine phosphatase activity, MAPK signaling pathway–plant, plant–pathogen interactions, and plant hormone signal transduction under drought stress, while downregulated DEGs were mainly involved in metal ion binding, transmembrane transporter activity, and phenylpropanoid biosynthesis. Additionally, 1441 TFs screened from DEGs were clustered into 64 TF families, such as AP2/ERF-ERF, bHLH, WRKY, NAC, MYB, and bZIP TF families. Genes related to physiological traits were closely related to starch and sucrose metabolism, phenylpropanoid biosynthesis, glutathione metabolism, and plant hormone signal transduction. In conclusion, the active oxygen metabolism system and the soluble protein of proso millet root could be regulated by the activity of protein serine/threonine phosphatase. AP2/ERF-ERF, bHLH, WRKY, NAC, MYB, and bZIP TF families were found to be closely associated with drought tolerance in proso millet root. This study will provide data to support a subsequent study on the function of the drought tolerance gene in proso millet. [ABSTRACT FROM AUTHOR]

Tobacco cembranoids, known for their anti-insect and antifungal properties, were shown to be mainly present on the surface of leaves and flowers, being biosynthesized by their trichomes. It remains unclear whether they could be biosynthesized in other organs without trichomes. Cembratrien-ol synthases (CBTSs) catalyze the conversion of GGPP to CBT-ols and thus play an important role in cembranoid biosynthesis. This study identified the CBTS family genes in tobacco and examined their spatiotemporal expression patterns. The CBTS genes showed diverse expression patterns in tobacco organs, with the majority highly expressed in leaves and a few highly expressed in flowers. The expression of CBTS genes were also correlated with the development of tobacco plants, and most of them showed the highest expression level at the budding stage. Furthermore, their expression is mediated by the JA (jasmonate) signaling in all tobacco organs. Several CBTS genes were found to be highly expressed in tobacco roots that have no trichomes, which prompted us to determine the cembranoid production in roots and other organs. GC-MS and UPLC assays revealed that cembranoids were produced in all tobacco organs, which was supported by the bioactivity assay results that almost all these CBTS enzymes could catalyze CBT-ol biosyntheis in yeast, and that the content ratio of CBT-ols and CBT-diols in tobacco roots was different to that in leaves. This work sheds insights into the expression profiles of tobacco CBTS genes and provides a feasibility to engineer tobacco roots for industrial production of cembranoids. [ABSTRACT FROM AUTHOR]

Introduction: The brachial plexus is a variable nerve plexus in its formation and branching pattern. Variations in the brachial plexus are not uncommon, and its area of supply and associations with other adjacent anatomical structures require clinical and surgical attention. Aim: To find out any anatomical variations in the formation and branching pattern of the brachial plexus in human foetuses. Materials and Methods: A morphometric prospective observational study was conducted from January 2020 to July 2022, on 30 stillborn or Intrauterine Dead (IUD) human foetuses were taken in the study. Gestation age from 28 to 40 weeks of gestation were included in the study, while those with gross anomalies or morphological anomalies of the cranium and vertebral column were excluded. The dissection includes vertical midline incision of the skin from the external occipital protuberance to the lower limit of the thoracic vertebra (T12). The skin was cut in the midline and reflected laterally. All superficial and deep muscles of the neck were dissect out to clear the cervical and thoracic parts of the vertebral column. The vertebral column was cut and opened to visualise the spinal cord and spinal nerves. Based on gestational age, the foetuses were divided into three groups: four in the first group (28-31 weeks), twelve in the second group (32-35 weeks), and fourteen in the third group (36-40 weeks) were divided for descriptive purposes. The gender of foetuses was determined based on the external genitalia. Results: A total of 60 brachial plexuses were dissected, of which 49 (81.66%) had the usual anatomical formation of the trunks: the upper trunk formed by C5 and C6 roots, the middle trunk by the C7 root, and the lower trunk by the C8 and T1 roots. Eleven (18.34%) plexuses presented variations in trunk formation. Specifically, 8 (13.3%) brachial plexuses were of the prefix type, where the upper trunk was formed by the C5 and C6 roots with an additional contribution from the C4 root; there was inter-branch communication between C6 and C7 in one (1.7%) case; the middle and lower trunks united by C7, C8, and T1 roots formed the lower trunk in another (1.7%) case, and finally, one (1.7%) case exhibited the post-fix type. Conclusion: In this study, out of the 30 foetuses examined, 11 showed variations, which is not uncommon. The prefix type of brachial plexus is quite common among the possible variations, although anatomists and neurosurgeons cannot ignore the possibility of other variations, like post-fix type or communication between the roots of the brachial plexuses. [ABSTRACT FROM AUTHOR]

In this study, we investigated the genetic mapping of root biomass and root/shoot ratio. We utilized a large (n = 345) bi‐parental recombinant inbred line (RIL) population from the 'Penny' × 'Yecora‐Rojo' cross to investigate the partitioning of biomass above‐ and belowground and to identify the quantitative trait loci (QTL) that influence root biomass and root/shoot ratio. Genotyping of 345 RILs by using genotyping by sequencing produced 2918 single‐nucleotide polymorphism markers by which a genome‐wide map of 3507 cM was constructed. Phenotyping was conducted in an augmented design with large pots in controlled environment. We identified two significant QTL regions, QRt.peye‐5A and QRt.peye‐5B, which control root biomass and the root/shoot ratio. QRt.peye‐5A, marking a 3.15 Mbp region on chromosome 5A, explained 11% of variations in root biomass and 9.5% of variations in root/shoot ratio, with the narrow region harbouring 28 genes. QRt.peye‐5B, marking a 12.2 Mbp region on chromosome 5B, explained 7% of variations in root/shoot ratio and harbours 104 genes. The root/shoot ratio enhancing alleles at QRt.peye‐5A and QRt.peye‐5B come from 'Penny' and 'Yecora‐Rojo' respectively. These QTL regions contains genes such as the two MADS box transcription factors on the 5A QTL that are candidate genes for Vrn1 locus, and other genes previously postulated for root traits such as a COBRA‐like COBL2 and landmark hormonal responses genes such as IAA16, IAA4 and BRI1, DREB2A‐INTERACTING PROTEIN2 (DRIP2) and bHLH92 which has a role in amelioration of stress conditions. [ABSTRACT FROM AUTHOR]

Aims: Fokienia hodginsii is a threatened conifer tree species, known as the dominant nursery-grown species capable of colonizing the challenging woodland environments in southern China due to its strong root penetrating ability. The ecological phenotype of Fokienia hodginsii is not well documented during its breeding process, which limits the potential planting area and its ecological function. This study aims to understand how Fokienia hodginsii associates with microbes to conduct its key ecological function and provide a theoretical basis for further improving the forest nursery management of Fokienia hodginsii. Methods: This study explored the ecological traits of 11 main Fokienia hodginsii provenances in a homogeneous garden experiment by analyzing their nutrient utilization strategies and associated microbial features in the rhizosphere soil and roots. Results: The study found that the paramount difference in the rhizosphere soil among provenances is in Ca and Fe content. Some microbial communities, namely Crenarchaeota, Verrucomicrobiota, and Desulfobacterota, were positively correlated with the amounts of the soil nutrient elements, whereas Abditibacteriota and Dependentiae were negatively correlated. The abundance of N- and Fe-related bacteria in the Fu Jian Chang Ting (FJCT) provenance was significantly higher than that in other provenances, while the C-, P-, K-, and Mg-related fungal communities, respectively, had higher abundances in the FJCT, Fu Jian Long Yan (FJLY), Fu Jian Gu Tian (FJGT), and Fu Jian Xian You (FJXY) provenances than the others. The impacts of the Gui Zhou Li Ping (GZLP), Hu Nan Dao Xian (HNDX), Jiang Xi Shang Yao (JXSY), and Guang Dong Shi Xing (GDSX) provenances on the rhizosphere soil are similar, but the differences in nutrient utilization arise from the plant itself. Conversely, the root nutrient contents of the FJCT, Fu Jian You Xi (FJYX), Fu Jian An Xi (FJAX), FJLY, Fu Jian De Hua (FJDH), FJGT, and FJXY provenances are highly correlated with soil nutrient features. Conclusions: For the native provenances, their economic traits are better than the exotic provenances. The native provenances are more sensitive to local soil conditions, so they should benefit more from human interventions, rendering them more suitable for artificial cultivation. The growth of the exotic provenances is less affected by the soil environment, making them better suited for the ecological transformation of forest stands and soil improvement. [ABSTRACT FROM AUTHOR]

Copyright of Research of Soil & Water Conservation is the property of Institute of Soil & Water Conservation, Chinese Academy of Sciences & Ministry of Water Resources and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background and Aims Internal root aeration is essential for root growth in waterlogged conditions. Aerenchyma provides a path for oxygen to diffuse to the roots. In most wetland species, including rice, a barrier to radial oxygen loss (ROL) allows more of the oxygen to diffuse to the root tip, enabling root growth into anoxic soil. Most dryland crops, including barley, do not form a root ROL barrier. We previously found that abscisic acid (ABA) signalling is involved in the induction of ROL barrier formation in rice during waterlogging. Although rice typically does not form a tight ROL barrier in roots in aerated conditions, an ROL barrier with suberized exodermis was induced by application of exogenous ABA. Therefore, we hypothesized that ABA application could also trigger root ROL barrier formation with hypodermal suberization in barley. Methods Formation of an ROL barrier was examined in roots in different exogenous ABA concentrations and at different time points using cylindrical electrodes and Methylene Blue staining. Additionally, we evaluated root porosity and observed suberin and lignin modification. Suberin, lignin and Casparian strips in the cell walls were observed by histochemical staining. We also evaluated the permeability of the apoplast to a tracer. Key Results Application of ABA induced suberization and ROL barrier formation in the adventitious roots of barley. The hypodermis also formed lignin-containing Casparian strips and a barrier to the infiltration of an apoplastic tracer (periodic acid). However, ABA application did not affect root porosity. Conclusions Our results show that in artificial conditions, barley can induce the formation of ROL and apoplastic barriers in the outer part of roots if ABA is applied exogenously. The difference in ROL barrier inducibility between barley (an upland species) and rice (a wetland species) might be attributable to differences in ABA signalling in roots in response to waterlogging conditions. [ABSTRACT FROM AUTHOR]

Peanut (Arachis hypogaea L.) is an important oilseed crop worldwide. However, soil salinization becomes one of the main limiting factors of peanut production. Therefore, developing salt-tolerant varieties and understanding the molecular mechanisms of salt tolerance is important to protect peanut yield in saline areas. In this study, we selected four peanut varieties with contrasting response to salt challenges with T1 and T2 being tolerance and S1 and S2 being susceptible. High-throughput RNA sequencing resulted in more than 314.63 Gb of clean data from 48 samples. We identified 12,057 new genes, 7,971of which have functional annotations. KEGG pathway enrichment analysis of uniquely expressed genes in salt-tolerant peanut revealed that upregulated genes in the root are involved in the MAPK signaling pathway, fatty acid degradation, glycolysis/gluconeogenesis, and upregulated genes in the shoot were involved in plant hormone signal transduction and the MAPK signaling pathway. Na+ content, K+ content, K+/ Na+, and dry mass were measured in root and shoot tissues, and two gene co-expression networks were constructed based on weighted gene co-expression network analysis (WGCNA) in root and shoot. In this study, four key modules that are highly related to peanut salt tolerance in root and shoot were identified, plant hormone signal transduction, phenylpropanoid biosynthesis, starch and sucrose metabolism, flavonoid biosynthesis, carbon metabolism were identified as the key biological processes and metabolic pathways for improving peanut salt tolerance. The hub genes include genes encoding ion transport (such as HAK8, CNGCs, NHX, NCL1) protein, aquaporin protein, CIPK11 (CBL-interacting serine/threonine-protein kinase 11), LEA5 (late embryogenesis abundant protein), POD3 (peroxidase 3), transcription factor, and MAPKKK3. There were some new salt-tolerant genes identified in peanut, including cytochrome P450, vinorine synthase, sugar transport protein 13, NPF 4.5, IAA14, zinc finger CCCH domain-containing protein 62, beta-amylase, fatty acyl-CoA reductase 3, MLO-like protein 6, G-type lectin S-receptor-like serine/threonine-protein kinase, and kinesin-like protein KIN-7B. The identification of key modules, biological pathways, and hub genes in this study enhances our understanding of the molecular mechanisms underlying salt tolerance in peanuts. This knowledge lays a theoretical foundation for improving and innovating salt-tolerant peanut germplasm. [ABSTRACT FROM AUTHOR]

Soil salinization poses a significant threat to agricultural productivity, necessitating innovative agronomic strategies to mitigate its impact. This study focuses on improving salt stress resistance in tomato plants through the application of silicon (Si) in roots. A greenhouse experiment was carried out under normal conditions (control, and 1 and 4 mM Si) and under salinity stress (salt control, and 1 and 4 mM Si). Various parameters were analyzed in leaves and roots. Under normal conditions, tomato plants grown in non-saline conditions exhibited some toxicity when exposed to Na2SiO3. As for the experiments under salt stress conditions, Si mitigated oxidative damage, preserving root cell membrane integrity. The concentration of malondialdehyde was reduced by 69.5%, that of proline was reduced by 56.4% and there was a 57.6% decrease in catalase activity for tomato plants treated with 1 mM Si under salt stress. Furthermore, Fe uptake and distribution, under salt conditions, increased from 91 to 123 mg kg−1, the same concentration as that obtained for the normal control. In all cases, the lower dose produced better results under normal conditions than the 4 mM dose. In summary, this research provides a potential application of Si in non-fertigated crop systems through a radicular pathway. [ABSTRACT FROM AUTHOR]

Background: In complex teeth like maxillary premolars, endodontic treatment success depends on a complete comprehension of root canal anatomy. The research on mandibular premolars' root canal anatomy has been extensive and well-documented in existing literature. However, there appears to be a notable gap in available data concerning the root canal anatomy of maxillary premolars. This study aimed to explore the root canal morphology of maxillary premolars using cone-beam computed tomography (CBCT) imaging, considering age and gender variations. Methods: From 500 patient CBCT scans, 787 maxillary premolar teeth were evaluated. The sample was divided by gender and age (10–20, 21–30, 31–40, 41–50, 51–60, and 61 years and older). Ahmed et al. classification system was used to record root canal morphology. Results: The most frequent classifications for right maxillary 1st premolars were 2MPM1 B1 L1 (39.03%) and 1MPM1 (2.81%), while the most frequent classifications for right maxillary 2nd premolars were 2MPM1 B1 L1 (39.08%) and 1MPM1 (17.85%). Most of the premolars typically had two roots (left maxillary first premolars: 81.5%, left maxillary second premolars: 82.7%, right maxillary first premolars: 74.4%, right maxillary second premolars: 75.7%). Left and right maxillary 1st premolars for classes 1MPM1 and 1MPM1–2−1 showed significant gender differences. For classifications 1MPM1 and 1MPM1–2−1, age-related changes were seen in the left and right maxillary first premolars. Conclusion: This study provides novel insights into the root canal anatomy of maxillary premolars within the Saudi population, addressing a notable gap in the literature specific to this demographic. Through CBCT imaging and analysis of large sample sizes, the complex and diverse nature of root canal morphology in these teeth among Saudi individuals is elucidated. The findings underscore the importance of CBCT imaging in precise treatment planning and decision-making tailored to the Saudi population. Consideration of age and gender-related variations further enhances understanding and aids in personalized endodontic interventions within this demographic. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aim is to carry out a comparative pharmacognostic study of the roots of A. tomentosum and A. minus with the root of A. lappa to confirm or deny their interchangeability. Materials and methods. A Delta optic BioLight 300 microscope (Poland) was used to study the macro- and microscopic features of plant raw materials. The method of gas chromatography-mass spectrometry was used to identify and quantify organic, including fatty, acids. Quantitative content of amount of organic acids, ascorbic acid, total polyphenols and amount of hydroxycinnamic acids was determined by using spectrophotometry. The content of polysaccharides was determined by the gravimetric method. The results. For the first time, a comparative morphological and anatomical study of the roots of A. tomentosum and A. minus in comparison with the root of A. lappa was carried out. As a result, a distinctive diagnostic microscopic feature of the roots was established: the shape of the receptacles of the schizogen type. For the first time, the component composition of organic, including fatty acids, for the roots of A. tomentosum and A. minus was determined in comparison with the root of A. lappa, which is the same. The content of 11 organics and 12 fatty acids in plant raw materials of 3 Arctium species was identified and determined. The quantitative content of the amount of organic acids, ascorbic acid, polysaccharides, total polyphenols, and amount of hydroxycinnamic acids in the roots of A. tomentosum and A. minus in comparison with the root of A. Lappa was established, and these indicators are comparable. Conclusions. For the first time, a comparative pharmacognostical study of the roots of A. tomentosum and A. minus in comparison with the root of A. lappa was carried out which showed minor differences between the roots of these Arctium species and confirms their interchangeability at this stage, especially when harvesting wild plant raw materials, when identification of the species at the botanical level is impossible [ABSTRACT FROM AUTHOR]

To improve nitrogen use efficiency (NUE) during rice cultivation, it is essential to comprehend the morphological and physiological traits of rice roots. However, in high-fertility black soil regions of Northeast China, the effects of combining biochar application with water-saving irrigation (WSI) conditions on rice root development and nitrogen utilization are still unknown. To address this knowledge gap, a combination of field experiments and 15N tracer micro-area investigations was conducted in this study. Four treatments were implemented: (i) controlled irrigation without biochar application (CB0); (ii) controlled irrigation with 2.5 t ha−1 biochar application (CB1); (iii) controlled irrigation with 12.5 t ha−1 biochar application (CB2); and (iv) controlled irrigation with 25 t ha–1 biochar application (CB3). Flooded irrigation conditions without biochar treatment (FB0) were used as the control. The primary objective of this research was to identify the mechanisms by which combined WSI conditions and biochar application affect rice root development and nitrogen utilization. Biochar application enhanced rice root morphological and physiological characteristics. Optimal biochar application increased the longest root length (RL), root volume (RV), root fresh weight (RFW), root active absorption area, root bleeding intensity, and root activity (RA) of rice while also optimizing the root–shoot ratio and facilitating nitrogen absorption by roots. These changes in root morphological and physiological characteristics facilitated the absorption of fertilizer-15N and soil nitrogen by rice roots, ultimately leading to improvements in rice yields and NUEs. Notably, the rice yields, NUE, nitrogen agronomic efficiency (NAE), and nitrogen partial factor productivity (NPFP) of CB2 plants were 16.45%, 39.42%, 24.48%, and 16.45% higher than those of FB0 plants, respectively. These results highlight the effectiveness of biochar application as a strategy to ensure food security and enhance NUE under WSI conditions. Furthermore, this study suggests that the recommended optimal application amount of biochar for the black soil area of Northeast China is 12.5 t ha−1. [ABSTRACT FROM AUTHOR]

Cinmethylin is a potential pre-emergence herbicide that could be used to control grass weeds in winter cereals. To determine the herbicidal activity of cinmethylin against common gramineous weeds in wheat fields in China and its level of safety on wheat, we conducted the following experiments: (i) assessing the efficacy of cinmethylin against 11 grass weeds and (ii) determining its safety against 19 wheat varieties. The results showed that cinmethylin had good herbicidal efficacy against annual bluegrass (Poa annua L.), shortawn foxtail (Alopecurus aequalis Sobol.), slender meadow foxtail (Alopecurus myosuroides Huds.), Japanese foxtail (Alopecurus japonicus Steud.), Italian ryegrass (Lolium multiflorum Lam.), British timothy (Phleum paniculatum Huds.), Asia Minor bluegrass (Polypogon fugax Nees ex Steud.), Helictotrichon tibeticum (Roshev.) Holub., and wild oat (Avena fatua L.), with a GR50 (the herbicide dose resulting in 50% growth inhibition) value of 4.50–99.21 g a.i. ha−1 in plant height and 1.43–70.34 g a.i. ha−1 in fresh weight. However, cinmethylin cannot control Japanese brome (Bromus japonicus L.) or Tausch's goatgrass (Aegilops tauschii Coss.) at a dose of 200 g a.i. ha−1. Different wheat varieties varied in their phytotoxicity to cinmethylin. Overall, there is a phytotoxicity risk when using cinmethylin on wheat, mainly to wheat roots, with a reduction in root length of 40.81–64.09% at a dose of 400 g a.i. ha−1. These findings indicate that the pre-emergence herbicide cinmethylin provides good efficacy against most grass weeds and may possess potential for weed management in wheat fields. However, attention should be given to the application dosage and the sensitivity of wheat varieties when using cinmethylin in wheat fields. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Simple Summary: The aim of this study was to analyze and compare the morphology of root canals in the carnassial teeth of German shepherds and mixed-breed dogs. Knowledge of the morphology of root canals is essential for a successful endodontic procedure. It has been determined that an apical delta, which represents a complex structure that consists of multiple cavities whose purpose is to allow the passing of nerves and blood vessels from the pulp cavity to the apex of the root, was present in 247 roots. There are four main types of apical delta. The most common type in superior fourth premolars was type II, with up to 10 apical ramifications, whereas type IIIA, with 10–20 apical ramifications, was most commonly present in inferior first molars. Root canal treatment of carnassial teeth in dogs is a common endodontic technique which aims to re-establish or maintain the health of the periapical tissues. In total, 43 dogs were used in this study. Root canal morphology was evaluated in 86 superior fourth premolars and 86 inferior first molars. Apical delta was present in 247 roots, while obliteration of the root canal was found in 11 roots. The most common type of apical delta of the roots of superior fourth premolars was type II, with up to 10 apical ramifications, while type IIIA, with 10–20 apical ramifications, was most commonly present in the roots of inferior first molars. Considering that knowledge of the morphology of root canals is essential for a successful endodontic procedure, the aim of this study was to analyze and compare the morphology of root canals in the carnassial teeth of German shepherds and mixed-breed dogs. Apical resection for the purpose of endodontic therapy of the superior fourth premolar and the inferior first molar is indicated at a length of 4 to 6 mm from the anatomical tip of the roots, which would completely remove the apical delta of these two teeth. [ABSTRACT FROM AUTHOR]

Copyright of Bulletin of Ablai Khan KazUIRandWL: Series 'Philological sciences' is the property of Kazakh Ablai Khan University of International Relations & World Languages and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Prataculturae Sinica is the property of Acta Prataculturae Sinica Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The identification of soybean genotypes tolerant to soil compaction makes it possible to reduce productivity loss under stress conditions. Added to this, the prior selection of these genotypes will result in greater assertiveness in the positioning of cultivars in the field. Thus, the objective was to evaluate the susceptibility of soybean genotypes to compaction in greenhouse and field conditions; verify which characteristics of seedlings under high resistance to root penetration are correlated with crop production in compacted soil; and to validate the substrate mechanical impedance method for evaluating the susceptibility of plant genotypes to soil compaction. Seeds of 20 genotypes were sown in a substrate mechanical impedance system under controlled conditions. The characteristics evaluated were total root length, total root surface area, mean root diameter, total root volume, taproot length, shoot length, root dry matter and seedling shoot dry matter. In the field experiment, half of the planting area was compacted, constituting two treatments, soil with and without compaction. The percentage of seedling emergence, initial plant height, stem diameter, number of nodes, internode length, number of lateral branches, shoot dry matter, final plant height, absolute and relative growth rate, number of pods, weight of 100 seeds and grain yield. In addition, the number of days between soybean sowing until plant flowering and grain harvest was recorded according to genotype and soil compaction level. In a controlled environment, genotypes tolerant to soil compaction show greater plasticity of root characteristics and smaller alterations in the shoot of seedlings. In the field, these genotypes show smaller reductions in growth rate, height, number of pods and grain yield. The shoot dry matter and the root dry matter of soybean seedlings in a mechanical impedance system present a positive and negative correlation, respectively, with soybean yield in compacted soil, indicating that the genetically determined susceptibility to soil compaction stress was similar throughout ontogenesis. The substrate mechanical impedance system used to evaluate the performance of soybean seedlings under stress, facilitates the decision‐making in breeding programs focused on identifying genotypes expressing soil compaction tolerance. [ABSTRACT FROM AUTHOR]

Copyright of Guangdong Agricultural Sciences is the property of South China Agricultural University, Guangdong Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Summary: Phloem sieve elements (PSE), the primary conduits collaborating with neighboring phloem pole pericycle (PPP) cells to facilitate unloading in Arabidopsis roots, undergo a series of developmental stages before achieving maturation and functionality. However, the mechanism that maintains the proper progression of these differentiation stages remains largely unknown.We identified a gain‐of‐function mutant altered phloem pole pericycle 1 Dominant (app1D), producing a truncated, nuclear‐localized active form of NAC with Transmembrane Motif 1‐like (NTL9). This mutation leads to ectopic expression of its downstream target CALLOSE SYNTHASE 8 (CalS8), thereby inducing callose accumulation, impeding SE differentiation, impairing phloem transport, and inhibiting root growth.The app1D phenotype could be reproduced by blocking the symplastic channels of cells within APP1 expression domain in wild‐type (WT) roots. The WT APP1 is primarily membrane‐tethered and dormant in the root meristem cells but entries into the nucleus in several cells in PPP near the unloading region, and this import is inhibited by blocking the symplastic intercellular transport in differentiating SE.Our results suggest a potential maintenance mechanism involving an APP1‐CalS8 module, which induces CalS8 expression and modulates symplastic communication, and the proper activation of this module is crucial for the successful differentiation of SE in the Arabidopsis root. [ABSTRACT FROM AUTHOR]

Protein disulfide isomerase (PDI, EC 5.3.4.1) is a thiol-disulfide oxidoreductase that plays a crucial role in catalyzing the oxidation and rearrangement of disulfides in substrate proteins. In plants, PDI is primarily involved in regulating seed germination and development, facilitating the oxidative folding of storage proteins in the endosperm, and also contributing to the formation of pollen. However, the role of PDI in root growth has not been previously studied. This research investigated the impact of PDI gene deficiency in plants by using 16F16 [2-(2-Chloroacetyl)-2,3,4,9-tetrahydro-1-methyl-1H-pyrido[3,4-b]indole-1-carboxylic acid methyl ester], a small-molecule inhibitor of PDI, to remove functional redundancy. The results showed that the growth of Arabidopsis roots was significantly inhibited when treated with 16F16. To further investigate the effects of 16F16 treatment, we conducted expression profiling of treated roots using RNA sequencing and a Tandem Mass Tag (TMT)-based quantitative proteomics approach at both the transcriptomic and proteomic levels. Our analysis revealed 994 differentially expressed genes (DEGs) at the transcript level, which were predominantly enriched in pathways associated with "phenylpropane biosynthesis", "plant hormone signal transduction", "plant−pathogen interaction" and "starch and sucrose metabolism" pathways. Additionally, we identified 120 differentially expressed proteins (DEPs) at the protein level. These proteins were mainly enriched in pathways such as "phenylpropanoid biosynthesis", "photosynthesis", "biosynthesis of various plant secondary metabolites", and "biosynthesis of secondary metabolites" pathways. The comprehensive transcriptome and proteome analyses revealed a regulatory network for root shortening in Arabidopsis seedlings under 16F16 treatment, mainly involving phenylpropane biosynthesis and plant hormone signal transduction pathways. This study enhances our understanding of the significant role of PDIs in Arabidopsis root growth and provides insights into the regulatory mechanisms of root shortening following 16F16 treatment. [ABSTRACT FROM AUTHOR]

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Trees form symbioses with ectomycorrhizal (ECM) fungi, maintained in part through mutual benefit to both organisms. Our understanding of the signaling events leading to the successful interaction between the two partners requires further study. This is especially true for understanding the role of volatile signals produced by ECM fungi. Terpenoids are a predominant class of volatiles produced by ECM fungi. While several ECM genomes are enriched in the enzymes responsible for the production of these volatiles (i.e., terpene synthases (TPSs)) when compared to other fungi, we have limited understanding of the biochemical products associated with each enzyme and the physiological impact of specific terpenes on plant growth. Using a combination of phylogenetic analyses, RNA sequencing, and functional characterization of five TPSs from two distantly related ECM fungi (Laccaria bicolor and Pisolithus microcarpus), we investigated the role of these secondary metabolites during the establishment of symbiosis. We found that despite phylogenetic divergence, these TPSs produced very similar terpene profiles. We focused on the role of P. microcarpus terpenes and found that the fungus expressed a diverse array of mono-, di-, and sesquiterpenes prior to contact with the host. However, these metabolites were repressed following physical contact with the host Eucalyptus grandis. Exposure of E. grandis to heterologously produced terpenes (enriched primarily in γ -cadinene) led to a reduction in the root growth rate and an increase in P. microcarpus–colonized root tips. These results support a very early putative role of fungal-produced terpenes in the establishment of symbiosis between mycorrhizal fungi and their hosts. [ABSTRACT FROM AUTHOR]

Introduction: Potato is the one of the important crop in the world. This plant in terms of consumption rate in the world levels is after rice and wheat. Thus, this crop is considered the maintain food security. However, potato influences the abiotic stress particularly drought and cold. The C-repeat binding factors (CBFs), also termed dehydration-responsive element-binding protein 1 (DREB1) family members, play crucial roles in the acquisition of stress tolerance, but in potato, the underlying mechanisms of stress tolerance remain elusive. The cold and drought stresses are the most abiotic stresses across the world causing to decrease of the crop yield. To gain insight into these mechanisms, CBF1 gene was selected from four potato and was assessed their expression under drought and cold stress conditions. Materials and methods: In order to investigate the effect of cold and drought on growth and development on potato, a experiment was conducted at the the Seed and Plant Improvement Institute located at Karaj, Iran. The experimental design used was randomized complete block design with three replications under drought and cold stress (normal and stressed). Gene expression of CBF1 gene was performed in different tissues (root, stem, leaves, and tuber) under control condiotin. Further, analysis of expression in root and leaves under stress condition. After 3 weeks after cultivated in Karaj, the water stress was induced by irrigation suppression in 2 weeks. sampling was performed 5 weeks after sprouting under drought. Under cold stress, analysis of expression in root and leaves was performed at the 24h and 48h after 3 weeks of sprouting. CBF1 gene was selected as one of important gene in the investigation of molecular mechanism under cold and heat stress in potato. Results and discussion: Average induced expression in response to cold suggested a correlation between potato CBF1 expression and the acquisition of cold tolerance in root. Whereas, the expression of this gene was decreased in leaves. Under drought stress, the expression of CBF1 gene was up-regulated in root. However, the expression rate of CBF1 decreased under stress. Responses that varied between sections may reflect tissue-specific stress tolerance mechanisms, suggesting an effect of ecological context on the development of CBF1 mediated stress tolerance in potato. In this study, the investigation of transcription factor binding sites, co-expressed genes, and gene ontology of CBF1 in potato was performed. This gene is involved in multiple biological processes and pathways. The analysis of promoter showed that CBF1 gene had the highest and the lowest number of the TFBS in MYB and WRKY, respectively. The present study revealed that the CBF1 gene expression in root was the higher than leaves. Conclusion: Overall, it can be concluded that CBF1 gene can contribute to cold and drought tolerance in potato and can be used for genetic and breeding manipulation to improve tolerance to stress. [ABSTRACT FROM AUTHOR]

The presence of epistasis in complex traits can be significant and affect the selection of segregating populations undesirably. The purpose of this study was to determine the epistatic genetic components that influence root and shoot traits of common bean and identify their effect on the performance of segregating populations. The field experiment consisted of 49 treatments (backcross progenies, parents and segregating populations in the F2 and F3 generations). The cross P1-BAF53 (Andean) × P2-IPR 88 Uirapuru (Mesoamerican) as reference. Six traits were taken into consideration: root distribution (%), first pod length (cm), number of grains (plot), plant height (cm), reproductive cycle (days) and number of basal branches (plot). For root distribution, first pod length and number of grains, the additive and additive × additive components were significant. On the contrary, for plant height, reproductive cycle and number of basal branches, no interaction component was observed. These results can be explained by the magnitude of variance determined for these traits. The additive × additive epistasis had a negative influence on the mean performance of segregating progenies. Contrariwise, transgressive segregation effects on plant height were observed as a function of genetic dominance deviation. Thus, additive × additive epistasis can hamper the success of a breeding program, for being associated with low-variability traits. This fact may be related with the number of crosses and/or the choice of genetically more distant parents for the program, and possibly with the presence of linked genes. [ABSTRACT FROM AUTHOR]