Search

Apicomplexans form a large phylum of parasitic protozoa, including the genera Plasmodium, Toxoplasma, and Cryptosporidium, the causative agents of malaria, toxoplasmosis, and cryptosporidiosis, respectively. They cause diseases not only in humans but also in animals, with dramatic consequences in agriculture. Most apicomplexans are vacuole-dwelling and obligate intracellular parasites; as they invade the host cell, they become encased in a parasitophorous vacuole (PV) derived from the host cellular membrane. This creates a parasite–host interface that acts as a protective barrier but also constitutes an obstacle through which the pathogen must import nutrients, eliminate wastes, and eventually break free upon egress. Completion of the parasitic life cycle requires intense remodeling of the infected host cell. Host cell subversion is mediated by a subset of essential effector parasitic proteins and virulence factors actively trafficked across the PV membrane. In the malaria parasite Plasmodium, a unique and highly specialized ATP-driven vacuolar secretion system, the Plasmodium translocon of exported proteins (PTEX), transports effector proteins across the vacuolar membrane. Its core is composed of the three essential proteins EXP2, PTEX150, and HSP101, and is supplemented by the two auxiliary proteins TRX2 and PTEX88. Many but not all secreted malarial effector proteins contain a vacuolar trafficking signal or Plasmodium export element (PEXEL) that requires processing by an endoplasmic reticulum protease, plasmepsin V, for proper export. Because vacuolar parasitic protein export is essential to parasite survival and virulence, this pathway is a promising target for the development of novel antimalarial therapeutics. This review summarizes the current state of structural and mechanistic knowledge on the Plasmodium parasitic vacuolar secretion and effector trafficking pathway, describing its most salient features and discussing the existing differences and commonalities with the vacuolar effector translocation MYR machinery recently described in Toxoplasma and other apicomplexans of significance to medical and veterinary sciences. [ABSTRACT FROM AUTHOR]

Rapeseed is an important oil product in China. China's current soybean trade issues with major soybean producing countries have caused a large decline in soybean import since 2017. This may bring the increasing needs of rapeseed import, which would have an impact on domestic production. However, our knowledge on the effects of international rapeseed trade on domestic production remains unknown. It is thus important to understand the pattern of rapeseed in China under this scenario, as it may provide necessary information for all relevant stakeholders. With this goal, this study aims to investigate the spatial and temporal patterns of winter rape in China's major winter rape production region, the Middle Reaches of the Yangtze River Valley (MYR), during 2003–2015 using time-series Moderate Resolution Imaging Spectroradiometer (MODIS) data. A decision tree according to the difference in enhanced vegetation index (EVI) profiles of land-cover types was built to extract winter rape. The results show that there is an essential decrease in both the number and density of winter rape patches under the opening global rapeseed market. There are significant hotspots of winter rape gain and loss, within which the loss dominated the trend. The significant cost advantage of rapeseed in the international market may largely reduce the domestic cultivation in China through telecoupling effects. Understanding the spatio-temporal dynamics of winter rape on the MYR has significant economic and policy implications and can provide great supports for the agricultural production, policy-making, and oil products trade in the international market. [ABSTRACT FROM AUTHOR]