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The use of transferable cross species/genera SSR markers is considered as cost effective strategy to ensure availability of markers in genomic resources of cotton. Forty one of 88 SSR primer pairs constitutes 46.59% [24 from safflower i.e 27.27% and 17 from pulses i.e 19.32%] were found transferable as they amplified SSR priming genomic regions of both the genotypes. Out of these 88 markers, 27 (30.68%) were shown monomorphism and 14 (15.91%) were shown polymorphism. Among amplified markers, 45.83% and 17.65% polymorphism was observed by these heterolgous markers of safflower and pulses respectively. [ABSTRACT FROM AUTHOR]

The article presents a study on utility of cross-species amplification of 34 rohu microsatellite loci in Labeo bata and their transferability in six other species of the cyprinidae family. It mentions that genomic DNA was extracted, and quantity and quality were checked following a standard protocol. Reportedly, the success rate of cross-species amplification was about 90 percent in the congeners.

Forty-eight primer pairs for chicken (Gallus gallus) microsatellite loci were tested in polymerase chain reaction (PCR) amplification of Japanese quail (Coturnix japonica) genomic DNA. Amplification products were obtained from 28 primer-pairs (58·3%) after optimizing the PCR conditions. Eleven (22·9%) of these generated specific products and 17 yielded non-specific amplification products. Eight markers (ADL0037, ADL0038, ADL0142, ADL0143, ADL0206, ADL0315, ADL0366, and HUJ0006) were polymorphic and three were monomorphic (ADL0023, ADL0024, and ADL0257) in four Japanese quail populations. Specific amplification products from each of the 11 PCR primers were sequenced. Seven of the eight polymorphic and one of three monomorphic markers contained simple tandem repeats. Six of these microsatellite loci (ADL0037, ADL0315, ADL0142, ADL0143, ADL0366 and ADL0257) may be homologous to the corresponding chicken loci from which the markers were developed. [ABSTRACT FROM AUTHOR]