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Background. Hepatocellular carcinoma (HCC) lacks effective treatments and has a poor prognosis. Therefore it is needed to develop more effective drug targets. Kinesin family member 11 (KIF11) has been reported to affect the progression of several cancers, and its high expression associates with the prognosis of patients. However, the relevant mechanisms of KIF11 in HCC progression have not been studied. Method. Through the cancer genome atlas (TCGA) database and immunohistochemical (IHC) staining of patients' specimens, we determined that KIF11 was highly expressed in HCC tissues and associated with prognosis. We established a KIF11 stably depleted hepatoma cell line, through cell-cloning experiments and cell counting kit-8 (CCK-8) assays to detect the effects on proliferation in vitro. The role of KIF11 in promoting cell proliferation was verified in mice. Result. The expression of KIF11 was negatively correlated with the overall survival (OS) and disease-free survival (DFS) and positively correlated with tumor size of HCC patients. KIF11 depletion inhibits cell proliferation and tumor growth in vitro and in vivo. Conclusion. KIF11 can be used as a positive correlation marker for HCC prognosis and served as a potential therapeutic target. [ABSTRACT FROM AUTHOR]

A high-performance centrifugal partition chromatography (HPCPC) method was developed for the preparative separation of patchouli alcohol. The separation was performed with a nonaqueous two-phase solvent system consisting of petroleum ether (bp.30-60 °C)-acetonitrile (1:1, v/v) in descending separation mode. A total of more than 2 g of patchouli alcohol was isolated from the essential oil (12.5 g) in 300 min. This component was separated and identified by comparing its retention time and MS data with the standard substance analyzed by GC/MS. The purity was more than 98%. About 450 mL petroleum ether and 550 mL acetonitrile were used for a single run, and 60% and 80% of petroleum ether and acetonitrile could be recovered for next run, respectively. [ABSTRACT FROM AUTHOR]