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Objective: The purpose of this study was to compare the analgesic effects of intercostal nerve block (ICNB) and local anesthetic infiltration (LAI) on postoperative pain and recovery following thoracoscopic resection of pulmonary bullae. Methods: A total of 160 patients undergoing thoracoscopic pulmonary bullae resection were randomly assigned to receive either ICNB (n = 80) or LAI (n = 80). An experienced anesthesiologist administered ultrasound guided ICNB at the T4 and T7 levels with 5 mL of 0.375% ropivacaine hydrochloride for the ICNB group. Instead, the LAI group received 10 mL of the same concentration of ropivacaine hydrochloride at the same concentration used for ICNB for infiltration anesthesia at the incision sites. Out of the initial cohort, 146 patients completed the study (ICNB group, n = 71; LAI group, n = 75). The collected data included preoperative clinical characteristics, visual analog scale (VAS) scores for pain at various time points post-surgery (6, 12, 24, 48, and 72 h). Additionally, the Quality of Recovery-15 (QoR-15) questionnaire was administered 24 h after surgery, and sleep quality was evaluated using the Pittsburgh Sleep Quality Index (PSQI). Results: No significant differences were found in drainage volume, use of additional analgesics, duration of chest tube placement, or hospital stay between the two groups. However, the ICNB group had significantly lower VAS scores and QoR-15 scores 24 h postoperatively (p < 0.05), indicating better pain management and recovery. The ICNB group also reported better sleep quality, as reflected by lower PSQI scores. Conclusion: ICNB provides superior analgesia compared to LAI after thoracoscopic resection of pulmonary bullae, significantly improving postoperative recovery. [ABSTRACT FROM AUTHOR]

Flexible and stretchable strain sensors are in high demand in sports performance monitoring, structural health monitoring, and biomedical applications. However, existing stretchable soft sensors, primarily based on soft polymer materials, often suffer from drawbacks, including high hysteresis, low durability, and delayed response. To overcome these limitations, a stretchable miniature fiber sensor comprised of a stretchable core tightly coiled with parallel conductive wires is introduced. This fiber sensor is flexible and stretchable while exhibiting low hysteresis, a remarkable theoretical resolution of 0.015%, a response time of <30 milliseconds, and excellent stability after extensive cycling tests of over 16 000 cycles. To understand and predict the capacitive sensor response of the proposed sensor, an analytical expression is derived and proved to have good agreements with both experimental results and numerical simulation. The potential of the strain sensor as a wearable device is demonstrated by embedding it into belts, gloves, and knee protectors. Additionally, the sensor can extend its applications beyond wearable devices, as demonstrated by its integration into bladder and life safety rope monitoring systems. The sensor is envisioned to have applications in the field of sports performance evaluations, health care monitoring, and structural safety assessments. [ABSTRACT FROM AUTHOR]

Diabetic nephropathy (DN) is a serious public health problem worldwide, and ferroptosis is deeply involved in the pathogenesis of DN. Prediabetes is a critical period in the prevention and control of diabetes and its complications, in which kidney injury occurs. This study aimed to explore whether ferroptosis would induce kidney injury in prediabetic mice, and whether vitamin D (VD) supplementation is capable of preventing kidney injury by inhibiting ferroptosis, while discussing the potential mechanisms. High-fat diet (HFD) fed KKAy mice and high glucose (HG) treated HK-2 cells were used as experimental subjects in the current study. Our results revealed that serious injury and ferroptosis take place in the kidney tissue of prediabetic mice; furthermore, VD intervention significantly improved the kidney structure and function in prediabetic mice and inhibited ferroptosis, showing ameliorated iron deposition, enhanced antioxidant capability, reduced reactive oxygen species (ROS) and lipid peroxidation accumulation. Meanwhile, VD up-regulated Klotho, solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4) expression, and down-regulated p53, transferrin receptor 1 (TFR1) and Acyl-Coenzyme A synthetase long-chain family member 4 (ACSL4) expression. Moreover, we demonstrated that HG-induced ferroptosis is antagonized by treatment of VD and knockdown of Klotho attenuates the protective effect of VD on ferroptosis in vitro. In conclusion, ferroptosis occurs in the kidney of prediabetic mice and VD owns a protective effect on prediabetic kidney injury, possibly by via the Klotho/p53 pathway, thus inhibiting hyperglycemia-induced ferroptosis. [ABSTRACT FROM AUTHOR]

Stochastic ultimate boundedness has always been a very important property, which plays an important role in the study of stochastic models. Thus, in this paper, we will study a stochastic periodic chemostat system, in which we assume that the nutrient input concentration and noise intensities are periodic. In order to make the stochastic periodic model have mathematical and biological significance, we will study a very important issue: the existence, uniqueness and ultimate boundedness of a global positive solution for a stochastic periodic chemostat system. [ABSTRACT FROM AUTHOR]

The International Traditional Medicine Clinical Trial Registry (ITMCTR) is a platform that aims to promote transparency and avoid selective reporting in traditional medicine (TM) clinical studies. It was officially recognized as a primary registry of the World Health Organization International Clinical Trial Registry Platform (WHO ICTRP) in 2023. The ITMCTR focuses on TM clinical studies and accepts various study designs, including randomized controlled trials, cohort studies, and case reports. It also serves as a database of registered clinical studies, providing valuable resources for researchers, policymakers, and the public. However, challenges such as inadequate dissemination and language barriers exist, and efforts are being made to address these issues and improve the platform. [Extracted from the article]

Methionine, an indispensable amino acid crucial for dietary balance, intricately governs metabolic pathways. Disruption in its equilibrium has the potential to heighten homocysteine levels in both plasma and tissues, posing a conceivable risk of inducing inflammation and detriment to the integrity of vascular endothelial cells. The intricate interplay between methionine metabolism, with a specific focus on S-adenosyl-L-methionine (SAM), and the onset of thoracic aortic dissection (TAD) remains enigmatic despite acknowledging the pivotal role of inflammation in this vascular condition. In an established murine model induced by β-aminopropionitrile monofumarate (BAPN), we delved into the repercussions of supplementing with S-adenosyl-L-methionine (SAM) on the progression of TAD. Our observations uncovered a noteworthy improvement in aortic dissection and rupture rates, accompanied by a marked reduction in mortality upon SAM supplementation. Notably, SAM supplementation exhibited a considerable protective effect against BAPN-induced degradation of elastin and the extracellular matrix. Furthermore, SAM supplementation demonstrated a robust inhibitory influence on the infiltration of immune cells, particularly neutrophils and macrophages. It also manifested a notable reduction in the inflammatory polarization of macrophages, evident through diminished accumulation of MHC-IIhigh macrophages and reduced expression of inflammatory cytokines such as IL1β and TNFα in macrophages. Simultaneously, SAM supplementation exerted a suppressive effect on the activation of CD4 + and CD8 + T cells within the aorta. This was evidenced by an elevated proportion of CD44- CD62L + naïve T cells and a concurrent decrease in CD44 + CD62L- effector T cells. In summary, our findings strongly suggest that the supplementation of SAM exhibits remarkable efficacy in alleviating BAPN-induced aortic inflammation, consequently impeding the progression of thoracic aortic dissection. [ABSTRACT FROM AUTHOR]

In this work, a series of new diarylpyrimidine derivatives as microtubule destabilizers were designed, synthesized, and evaluated for anticancer activities. Based on restriction configuration strategy, we introduced the pyrimidine moiety containing the hydrogen-bond acceptors as c/s-olefin bond of CA-4 analogs to improve structural stability. Compounds 11a-t exerted antiproliferative activities against three human cancer cell lines (SGC-7901, HeLa, and MCF-7), due to tubulin polymerization inhibition, showing high selectivity toward cancer cells in comparison with non-tumoral HSF cells, as evidenced by MTT assays. In mechanistic investigations, compound 11s remarkably inhibited tubulin polymerization and disorganized microtubule in SGC-7901 cells by binding to tubulin. Moreover, 11s caused G2/M phase cell cycle arrest in SGC-7901 cells in a concentration-dependent manner. Furthermore, molecular modeling analysis revealed that 11s interacts with tubulin through binding to the colchicine site. In addition, the prediction of physicochemical properties disclosed that 11s conformed well to the Lipinski's rule of five. This work offered a fresh viewpoint for the discovery of new tubulintargeting anticancer drugs. [ABSTRACT FROM AUTHOR]

Objective: Surgical site infection (SSI) after spinal surgery is still a persistent worldwide health concern as it is a worrying and devastating complication. The number of samples in previous studies is limited and the role of conservative antibiotic therapy has not been established. This study aims to evaluate the clinical efficacy and feasibility of empirical antibiotic treatment for suspected early‐onset deep spinal SSI. Methods: We conducted a retrospective study to identify all cases with suspected early‐onset deep SSI after lumbar instrumented surgery between January 2009 and December 2018. We evaluated the potential risks for antibiotic treatment, examined the antibiotic treatment failure rate, and applied logistic regression analysis to assess the risk factors for empirical antibiotic treatment failure. Results: Over the past 10 years, 45 patients matched the inclusion criteria. The success rate of antibiotic treatment was 62.2% (28/45). Of the 17 patients who failed antibiotic treatment, 16 were cured after a debridement intervention and the remaining one required removal of the internal fixation before recovery. On univariate analysis, risk factors for antibiotic treatment failure included age, increasing or persisting back pain, wound dehiscence, localized swelling, and time to SSI (cut‐off: 10 days). Multivariate analysis revealed that infection occurring 10 days after primary surgery and wound dehiscence were independent risk factors for antibiotic treatment failure. Conclusion: Appropriate antibiotic treatment is an alternative strategy for suspected early‐onset deep SSI after lumbar instrumented surgery. Antibiotic treatment for suspected SSI occurring within 10 days after primary surgery may improve the success rate of antibiotic intervention. Patients with wound dehiscence have a significantly higher likelihood of requiring surgical intervention. [ABSTRACT FROM AUTHOR]

The eutectic mixtures consist of dl-menthol and acetic acid were prepared at five molar ratios (3:1, 2:1, 1:1, 1:2, 1:3). The vibrating-tube densimeter was used to measure the density of the DL-menthol/acetic acid mixtures, and the measurements were carried out from 293.15 K to 363.15 K and pressures from 0.1 MPa to 70 MPa. The measured densities of each dl-menthol/acetic acid mixture at different temperature and pressure were correlated by the Tait equation. In addition, the derived properties of dl-menthol/acetic acid mixtures including isothermal compressibility, isobaric thermal expansivity, and internal pressure were calculated. The effects of temperature, pressure, and molar ratios on the derived properties were compared and analyzed. [ABSTRACT FROM AUTHOR]

Multi‐agent reinforcement learning relies on reward signals to guide the policy networks of individual agents. However, in high‐dimensional continuous spaces, the non‐stationary environment can provide outdated experiences that hinder convergence, resulting in ineffective training performance for multi‐agent systems. To tackle this issue, a novel reinforcement learning scheme, Mutual Information Oriented Deep Skill Chaining (MioDSC), is proposed that generates an optimised cooperative policy by incorporating intrinsic rewards based on mutual information to improve exploration efficiency. These rewards encourage agents to diversify their learning process by engaging in actions that increase the mutual information between their actions and the environment state. In addition, MioDSC can generate cooperative policies using the options framework, allowing agents to learn and reuse complex action sequences and accelerating the convergence speed of multi‐agent learning. MioDSC was evaluated in the multi‐agent particle environment and the StarCraft multi‐agent challenge at varying difficulty levels. The experimental results demonstrate that MioDSC outperforms state‐of‐the‐art methods and is robust across various multi‐agent system tasks with high stability. [ABSTRACT FROM AUTHOR]

Nonenzymatic glycosylation of proteins can generate advanced glycosylation end products, which are closely associated with the pathogenesis of certain chronic physiological diseases and aging. In this study, we characterized the covalent binding of cyanidin‐3‐glucoside (C3G) to bovine serum albumin (BSA) and investigated the mechanism by which this covalent binding inhibits the nonenzymatic glycosylation of BSA. The results indicated that the covalent interaction between C3G and BSA stabilized the protein's secondary structure. Through liquid chromatography‐electrospray ionization tandem mass spectrometry analysis, we identified the covalent binding sites of C3G on BSA as lysine, arginine, asparagine, glutamine, and cysteine residues. This covalent interaction significantly suppressed the nonenzymatic glycosylation of BSA, consequently reducing the formation of nonenzymatic glycosylation products. C3G competitively binds to nonenzymatic glycosylation sites (e.g., lysine and arginine) on BSA, thereby impeding the glycosylation process and preventing the misfolding and structural alterations of BSA induced by fructose. Furthermore, the covalent attachment of C3G to BSA preserves the secondary structure of BSA and hinders subsequent nonenzymatic glycosylation events. [ABSTRACT FROM AUTHOR]

Background: The research aims to explore the characteristics of intestinal flora, nutritional status and immune function in patients with different types of obese colon cancer. Methods: A retrospective analysis is conducted on 64 cases of obese colon cancer diagnosed from June 2018 to January 2020. According to the histological staging of the cancer, they are classified into adenocarcinoma, adenosquamous carcinoma and undifferentiated carcinoma, with corresponding cases of 24, 22 and 18, respectively. The intestinal flora (Bifidobacterium, Lactobacillus, Enterococcus faecalis, Escherichia coli, and yeast), nutritional status (Hb, Alb, PA, TFN, and PNI), immune function (IgG, IgM, IgA, CD4+, CD8+, and CD4+/CD8+) are analyzed in the different groups of patients. Survival curves are evaluated by Kaplan-Meier method and log-rank test for tumour death, local recurrence, and distant metastasis. Results: There were no statistically significant differences in intestinal flora (Bifidobacterium, Lactobacillus, Enterococcus faecalis, Escherichia coli, and yeast), nutritional status (Hb, Alb, PA, TFN, and PNI) and immune function (IgG, IgM, IgA, CD4+, CD8+, and CD4+/CD8+) between different groups. There was a significant correlation between intestinal flora, nutritional status and immune function for all three. The survival curves of tumour death, local recurrence and distant metastasis in different groups of obese colon cancer patients were statistically significant. The tumor mortality rate, local recurrence, and distant metastasis rate in adenocarcinoma were 78.65%, 54.25% and 48.26% respectively. Conclusion: There are differences in intestinal flora, nutritional status and immune function among different types of obese colon cancer patients, but adenocarcinoma has the least benefit in intestinal flora, poor nutritional status, and weakest immune function. [ABSTRACT FROM AUTHOR]

Frank's sign (FS) refers to a diagonal skin fold between the tragus and the outer edge of the earlobe. FS has been identified as an independent variable in coronary artery disease (CAD). Young patients with FS and previous myocardial infarction are still rarely reported in clinical studies. We report the case of a 49‐year‐old male smoker and diabetic, with a history of myocardial infarction, who presented to the emergency department due to 2 h typical cardiac chest pain. His urgent electrocardiography (ECG) showed ST elevation, and cardiac biomarkers were elevated after admission. A diagonal earlobe crease (DELC) was observed in physical tests. The preliminary diagnosis considered acute coronary syndrome (ACS). Subsequently, acute coronary artery angiography demonstrated a slit‐like contrast defect in the proximal right coronary artery (RCA), with stenosis and occlusion in the distal segment. The percutaneous coronary intervention (PCI) was performed immediately. The patient's chest pain symptoms were relieved significantly after intervention. Our case indicates that FS should be highly regarded as a routine cardiovascular clinical examination, which can be effortlessly applied and be easily interpreted for screening to suspect the presence of ischemic heart disease. This may set strategies for primary screening in a younger population and prompt early diagnosis and treatment. [ABSTRACT FROM AUTHOR]

Magnetic metal absorbing materials have exhibited excellent absorptance performance. However, their applications are still limited in terms of light weight, low thickness and wide absorption bandwidth. To address this challenge, we design a broadband and low-profile multilayer absorber using cobalt–iron (CoFe) alloys doped with rare earth elements (REEs) lanthanum (La) and Neodymium (Nd). An improved estimation of distribution algorithm (IEDA) is employed in conjunction with a mathematical model of multilayer absorbing materials (MAMs) to optimize both the relative bandwidth with reflection loss (RL) below −10 dB and the thickness. Firstly, the absorption performance of CoFe alloys doped with La/Nd with different contents is analysed. Subsequently, IEDA is introduced based on a mathematical model to achieve an optimal MAM design that obtains a balance between absorption bandwidth and thickness. To validate the feasibility of our proposed method, a triple-layer MAM is designed and optimized to exhibit wide absorption bandwidth covering C, X, and Ku bands (6.16–12.82 GHz) and a total thickness of 2.39 mm. Then, the electromagnetic (EM) absorption mechanisms of the triple-layer MAMs are systematically investigated. Finally, the triple-layer sample is further fabricated and measured. The experimental result is in good agreement with the simulated result. This paper presents a rapid and efficient optimization method for designing MAMs, offering promising prospects in microwave applications, such as radar-stealth technology, EM shielding, and reduced EM pollution for electronic devices. [ABSTRACT FROM AUTHOR]

When performing three-dimensional braiding, the carrier base of a conventional braiding machine can only be move on a single track, but cannot be switched between tracks. In this study, a three-dimensional braiding machine with orbital change interlocking is designed to realize the carrier track change. Aiming at the crucial components in track change interlocking mechanism, the track change process is separated into seven periods, and the driving function of slider and electric rotary are obtained. The kinematics model of track change interlocking mechanism is established, the velocity, position and trajectory of carrier base are analyzed on ADAMS simulation software. The results show that the carrier base maximum velocity is −226.51 mm/s, the carrier base maximum position is 68.30 mm, and the carrier base maximum vibration is 0.14 to 1.08 mm in track change process, prototype test indicate that three-dimensional braiding machine with track change interlocking is feasible, provides reference for the future design of three-dimensional braiding machine. [ABSTRACT FROM AUTHOR]

Proteolysis-targeting chimeras (PROTACs) technology has garnered significant attention over the last 10 years, representing a burgeoning therapeutic approach with the potential to address pathogenic proteins that have historically posed challenges for traditional small-molecule inhibitors. PROTACs exploit the endogenous E3 ubiquitin ligases to facilitate degradation of the proteins of interest (POIs) through the ubiquitin–proteasome system (UPS) in a cyclic catalytic manner. Despite recent endeavors to advance the utilization of PROTACs in clinical settings, the majority of PROTACs fail to progress beyond the preclinical phase of drug development. There are multiple factors impeding the market entry of PROTACs, with the insufficiently precise degradation of favorable POIs standing out as one of the most formidable obstacles. Recently, there has been exploration of new-generation advanced PROTACs, including small-molecule PROTAC prodrugs, biomacromolecule-PROTAC conjugates, and nano-PROTACs, to improve the in vivo efficacy of PROTACs. These improved PROTACs possess the capability to mitigate undesirable physicochemical characteristics inherent in traditional PROTACs, thereby enhancing their targetability and reducing off-target side effects. The new-generation of advanced PROTACs will mark a pivotal turning point in the realm of targeted protein degradation. In this comprehensive review, we have meticulously summarized the state-of-the-art advancements achieved by these cutting-edge PROTACs, elucidated their underlying design principles, deliberated upon the prevailing challenges encountered, and provided an insightful outlook on future prospects within this burgeoning field. [ABSTRACT FROM AUTHOR]

Highlights: The frequency and intensity of multi-magnetic resonance are freely regulated by co-doping La3+ and Zr4+ ions. Zr4+ occupation is elaborately modified for promoting the portion of polarization/conduction loss to increase profoundly. The optimized electromagnetic characteristics lead to an ultra-wide bandwidth of 12.5+ GHz around millimeter-wave atmospheric window. The utilization of electromagnetic waves is rapidly advancing into the millimeter-wave frequency range, posing increasingly severe challenges in terms of electromagnetic pollution prevention and radar stealth. However, existing millimeter-wave absorbers are still inadequate in addressing these issues due to their monotonous magnetic resonance pattern. In this work, rare-earth La3+ and non-magnetic Zr4+ ions are simultaneously incorporated into M-type barium ferrite (BaM) to intentionally manipulate the multi-magnetic resonance behavior. By leveraging the contrary impact of La3+ and Zr4+ ions on magnetocrystalline anisotropy field, the restrictive relationship between intensity and frequency of the multi-magnetic resonance is successfully eliminated. The magnetic resonance peak-differentiating and imitating results confirm that significant multi-magnetic resonance phenomenon emerges around 35 GHz due to the reinforced exchange coupling effect between Fe3+ and Fe2+ ions. Additionally, Mössbauer spectra analysis, first-principle calculations, and least square fitting collectively identify that additional La3+ doping leads to a profound rearrangement of Zr4+ occupation and thus makes the portion of polarization/conduction loss increase gradually. As a consequence, the La3+–Zr4+ co-doped BaM achieves an ultra-broad bandwidth of 12.5 + GHz covering from 27.5 to 40 + GHz, which holds remarkable potential for millimeter-wave absorbers around the atmospheric window of 35 GHz. [ABSTRACT FROM AUTHOR]

The development of efficient hydrogen release and storage processes to provide environmentally friendly hydrogen solutions for mobile energy storage systems (MESS) stands as one of the most challenging tasks in addressing the energy crisis and environmental degradation. The catalytic dehydrogenative coupling of methanol and amines (DCMA) and its reverse are featured by high capacity for hydrogen release and storage, enhanced capability to purify the produced hydrogen, avoidance of carbon emissions and singular product composition, offering the environmentally and operationally benign strategy of overcoming the challenges associated with MESS. Particularly, the cycle between these two processes within the same catalytic system eliminates the need for collecting and transporting spent fuel back to a central facility, significantly facilitating easy recharging. Despite the promising attributes of the above strategy for environmentally friendly hydrogen solutions, challenges persist, primarily due to the high thermodynamic barriers encountered in methanol dehydrogenation and amide hydrogenation. By systematically summarizing various reaction mechanisms and pathways involving Ru-, Mn-, Fe-, and Mo-based catalytic systems in the development of catalytic DCMA and its reverse and the cycling between the two, this review highlights the current research landscape, identifies gaps, and suggests directions for future investigations to overcome these challenges. Additionally, the critical importance of developing efficient catalytic systems that operate under milder conditions, thereby facilitating the practical application of DCMA in MESS, is also underscored. [ABSTRACT FROM AUTHOR]

To establish the endovascular repair and prognosis of patients with aorto-iliac aneurysm and Brucella abortus infection. Methods: From September 2018 to September 2021, seven cases of Brucella abortus infection with aorto-iliac aneurysm were treated by the endovascular aneurysm repair (EVAR) procedure. Clinical and imaging data were collected to evaluate the therapeutic results, including body temperature, blood culture, imaging manifestations, stent patency and endoleak during the postoperative and follow-up periods. Results: Except for one patient who died of acute hematemesis and hematochezia just after the admission, seven patients were treated successfully. The aneurysms were completely excluded, and all stent grafts were patent. Patients were followed up for 12– 32 months, with an average follow-up of 18.5 ± 9.1 months. There were no cases of endoleak, infection recurrence, gluteal muscle ischemia or spinal cord ischemia during the follow-up period. Conclusion: It is feasible to treat Brucella abortus-infected aneurysms with the EVAR procedure. The results were optimistic in the short and medium-term. The application of sensitive antibiotics before and after the operation is the cornerstone of endovascular therapy. However, the long-term results require further follow-up. [ABSTRACT FROM AUTHOR]

A collision-free path planning method is proposed based on learning from demonstration (LfD) to address the challenges of cumbersome manual teaching operations caused by complex action of yarn storage, variable mechanism positions, and limited workspace in preform weaving. First, by utilizing extreme learning machines (ELM) to autonomously learn the teaching data of yarn storage, the mapping relationship between the starting and ending points and the teaching path points is constructed to obtain the imitation path with similar storage actions under the starting and ending points of the new task. Second, an improved rapidly expanding random trees (IRRT) method with adaptive direction and step size is proposed to expand path points with high quality. Finally, taking the spatical guidance point of imitation path as the target direction of IRRT, the expansion direction is biased toward the imitation path to obtain a collision-free path that meets the action yarn storage. The results of different yarn storage examples show that the ELM-IRRT method can plan the yarn storage path within 2s–5s when the position of the mechanism changes in narrow spaces, avoiding tedious manual operations that program the robot movements, which is feasible and effective. [ABSTRACT FROM AUTHOR]

The surface tension of the mixtures of R1234ze(E) with two POE lubricant oils (RL68H and SL32S) was measured at temperatures from 278 K to 338 K. The experimental system which is established based on differential capillary rise method was used for the present measurement. The surface tension enhancement rate of R1234ze(E)/RL68H and R1234ze(E)/SL32S mixtures compared to pure R1234ze(E) was discussed. In addition, the surface tension data were correlated as a function of temperature and lubricant oil mass fraction. The average absolute deviation for R1234ze(E)/RL68H and R1234ze(E)/SL32S mixtures between experimental data and calculated values was 0.08 mN·m−1 and 0.06 mN·m−1, respectively. [ABSTRACT FROM AUTHOR]

Stress tolerance plays a vital role in ensuring the effectiveness of piezoresistive sensing films used in flexible pressure sensors. However, existing methods for enhancing stress tolerance employ dome-shaped, wrinkle-shaped, and pyramidal-shaped microstructures in intricate molding and demolding processes, which introduce significant fabrication challenges and limit the sensing performance. To address these shortcomings, this paper presents periodic microslits in a sensing film made of multiwalled carbon nanotubes and polydimethylsiloxane to realize ultrahigh stress tolerance with a theoretical maximum of 2.477 MPa and a sensitivity of 18.092 kPa−1. The periodic microslits permit extensive deformation under high pressure (e.g., 400 kPa) to widen the detection range. Moreover, the periodic microslits also enhance the sensitivity based on simultaneously exhibiting multiple synapses within the sensing interface and between the periodic sensing cells. The proposed solution is verified by experiments using sensors based on the microslit strategy for wind direction detection, robot movement sensing, and human health monitoring. In these experiments, vehicle load detection is achieved for ultrahigh pressure sensing under an ultrahigh pressure of over 400 kPa and a ratio of the contact area to the total area of 32.74%. The results indicate that the proposed microslit strategy can achieve ultrahigh stress tolerance while simplifying the fabrication complexity of preparing microstructure sensing films. [ABSTRACT FROM AUTHOR]

In order to reduce the weight of the metal robot, the big arm of the 6-DOF robot is taken as the research object, and combined the forming process of the three-dimensional braided carbon fiber composite material, a lightweight design method of the manipulator based on the CFRP/QT (carbon fiber reinforced polymer/castiron QT 500-7) hybrid structure is proposed to design the structure of the manipulator. Through the static analysis of the hybrid structure manipulator by the finite element analysis method, the final scheme of the hybrid structure of the mechanical arm is obtained under the requirements of strength and stiffness. At the same time, the dynamic simulation results show that the robot equipped with a 24% weight reduction hybrid structure manipulator has good motion performance, and the driving torque of the hybrid structure manipulator robot at the J1 joint after weight reduction is smaller. [ABSTRACT FROM AUTHOR]

Currently, the obvious side effects of anti-tumor drugs, premature drug release, and low tumor penetration of nanoparticles have largely reduced the therapeutic effects of chemotherapy. A drug delivery vehicle (MCN-SS-GQDs) was designed innovatively. For this, the mesoporous carbon nanoparticles (MCN) with the capabilities of superior photothermal conversion efficiency and high loading efficiency were used as the skeleton structure, and graphene quantum dots (GQDs) were gated on the mesopores via disulfide bonds. The doxorubicin (DOX) was used to evaluate the pH-, GSH-, and NIR-responsive release performances of DOX/MCN-SS-GQDs. The disulfide bonds of MCN-SS-GQDs can be ruptured under high glutathione concentration in the tumor microenvironment, inducing the responsive release of DOX and the detachment of GQDs. The local temperature of a tumor increases significantly through the photothermal conversion of double carbon materials (MCN and GQDs) under near-infrared light irradiation. Local hyperthermia can promote tumor cell apoptosis, accelerate the release of drugs, and increase the sensitivity of tumor cells to chemotherapy, thus increasing treatment effect. At the same time, the detached GQDs can take advantage of their extremely small size (5–10 nm) to penetrate deeply into tumor tissues, solving the problem of low permeability of traditional nanoparticles. By utilizing the photothermal properties of GQDs, synergistic photothermal conversion between GQDs and MCN was realized for the purpose of synergistic photothermal treatment of superficial and deep tumor tissues. [ABSTRACT FROM AUTHOR]

Fruit color affects its commercial value. β-carotene is the pigment that provides color for many fruits and vegetables. However, the molecular mechanism of β-carotene metabolism during apricot ripening is largely unknown. Here, we investigated whether β-carotene content affects apricot fruit color. First, the differences in β-carotene content between orange apricot 'JTY' and white apricot 'X15' during nine developmental stages (S1–S9) were compared. β-carotene contents highly significantly differed between 'JTY' and 'X15' from S5 (color transition stage) onwards. Whole-transcriptome analysis showed that the β-carotene synthesis genes 15-cis-phytoene desaturase (PaPDS) and 15-cis-phytoene synthase (PaPSY) significantly differed between the two cultivars during the color transition stage. There was a 5 bp deletion in exon 11 of PaPDS in 'X15', which led to early termination of amino acid translation. Gene overexpression and virus-induced silencing analysis showed that truncated PaPDS disrupted the β-carotene biosynthesis pathway in apricot pulp, resulting in decreased β-carotene content and a white phenotype. Furthermore, virus-induced silencing analysis showed that PaPSY was also a key gene in β-carotene biosynthesis. These findings provide new insights into the molecular regulation of apricot carotenoids and provide a theoretical reference for breeding new cultivars of apricot. [ABSTRACT FROM AUTHOR]

Single-screw extruders and injection molding machines are essential equipment in polymer processing. It is of great importance for the optimization of operating parameters and the design of extrusion screw to predict the throughput of an extruder and the metering time of an injection molding machine according to the geometric parameters of the screw, operating parameters, and the rheological behavior of materials. Most polymer melts exhibit non-Newtonian behavior. The lattice Boltzmann method has many advantages in simulating the flow of non-Newtonian fluids. Herein, the dimensionless velocity profiles and dimensionless volumetric flow rate of generalized Newtonian fluids in a screw channel have been studied using the two-relaxation-time lattice Boltzmann method (TRT–LBM). The numerical results of power-law fluids are in good agreement with the analytical solutions, which verifies the validity of TRT–LBM. Through research, the change rule of the dimensionless volumetric flow rate of Bingham fluids with dimensionless pressure gradient has been obtained. It was found that the rheological properties of polymer melts and the dimensionless pressure gradient significantly affect the dimensionless velocity profiles and dimensionless volumetric flow rate. The dimensionless volume flow rate has some unexpected changes with the increase of the dimensionless pressure gradient. This study can provide theoretical guidance for the optimization of operating parameters and the design of extrusion screws. [ABSTRACT FROM AUTHOR]

The expansion of available information in location-based social networks (LBSNs) has led to information overload, making it urgent to discover users' next point-of-interest (POI). Some existing works only consider certain modal information in LBSNs and do not transform them into high-dimensional structures, which hinders the alleviation of the data sparsity problem. Moreover, many approaches rely solely on social relationships, making it difficult to recommend POIs to new users without association information. To tackle these challenges, we propose a social- and spatial–temporal-aware next point-of-Interest (SSTP) recommendation model. SSTP uses two feature encoders based on self-attention mechanism and gate recurrent unit to model users' check-in enhancement sequence hierarchically. We also design a random neighborhood sampling approach to mine user social relationships, thus alleviating the user cold start problem. Finally, we propose a geographical-aware graph attention network to learn the sensitivity of users to distance. Extensive experiments on two real-world datasets show that SSTP outperforms state-of-the-art models, improving Hit@k by 2.26–6.55 % and MAP@k by 3.49–6.55 % . Moreover, SSTP has better performance on sparse data, with an average improvement of 6.09 % on the Hit@k. The code can be downloaded at https://github.com/Rih0/sstp. [ABSTRACT FROM AUTHOR]

Purpose: We aimed to evaluate whether pulmonary fibrosis occurs in type 2 diabetes rat models and whether VD3 can prevent it by inhibiting pyroptosis. Methods: Sprague-Dawley rats were assigned to normal control (NC), diabetic model control (MC), low-dose VD3 (LVD), medium-dose VD3 (MVD), high-dose VD3 (HVD) and metformin positive control (PC) groups. Type 2 diabetes model was induced by a high-sugar, high-fat diet combined with STZ injection, and subsequently intervened with VD3 or metformin for 10 weeks. Blood glucose, body weight, food intake, water intake, urine volume, morphology, lung hydroxyproline level, immunohistochemistry, TUNEL staining, inflammatory cytokines secretion and related protein expression were analyzed. Results: Diabetic rats exhibited significant impairments in fasting blood glucose, insulin resistance, body weight, food intake, water intake, and urine volume. While morphological parameters, diabetic rats exhibited severe lung fibrosis. Intriguingly, VD3 intervention reversed, at least in part, the diabetes-induced alterations. The expression of pyroptosis-related proteins was up-regulated in diabetic lungs whereas the changes were reversed by VD3. In the meanwhile, SIRT3 expression was down-regulated in diabetic lungs while VD3 up-regulated it. Conclusion: Fibrotic changes were observed in diabetic rat lung tissue and our study indicates that VD3 may effectively ameliorate diabetic pulmonary fibrosis via SIRT3-mediated suppression of pyroptosis. [ABSTRACT FROM AUTHOR]

The apricot (Prunus armeniaca L.) is a fruit that belongs to the Rosaceae family; it has a unique flavor and is of important economic and nutritional value. The composition and content of soluble sugars and organic acids in fruit are key factors in determining the flavor quality. However, the molecular mechanism of sugar and acid accumulation in apricots remains unclear. We measured sucrose, fructose, glucose, sorbitol, starch, malate, citric acid, titratable acid, and pH, and investigated the transcriptome profiles of three apricots (the high-sugar cultivar 'Shushanggan', common-sugar cultivar 'Sungold', and low-sugar cultivar 'F43') at three distinct developmental phases. The findings indicated that 'Shushanggan' accumulates a greater amount of sucrose, glucose, fructose, and sorbitol, and less citric acid and titratable acid, resulting in a better flavor; 'Sungold' mainly accumulates more sucrose and less citric acid and starch for the second flavor; and 'F43' mainly accumulates more titratable acid, citric acid, and starch for a lesser degree of sweetness. We investigated the DEGs associated with the starch and sucrose metabolism pathways, citrate cycle pathway, glycolysis pathway, and a handful of sugar transporter proteins, which were considered to be important regulators of sugar and acid accumulation. Additionally, an analysis of the co-expression network of weighted genes unveiled a robust correlation between the brown module and sucrose, glucose, and fructose, with VIP being identified as a hub gene that interacted with four sugar transporter proteins (SLC35B3, SLC32A, SLC2A8, and SLC2A13), as well as three structural genes for sugar and acid metabolism (MUR3, E3.2.1.67, and CSLD). Furthermore, we found some lncRNAs and miRNAs that regulate these genes. Our findings provide clues to the functional genes related to sugar metabolism, and lay the foundation for the selection and cultivation of high-sugar apricots in the future. [ABSTRACT FROM AUTHOR]

The third robotic arm (drive housing) of a six-axis industrial robot often has a vertical inwardly concave surface to facilitate the installation of spatially vertical drive motors. With the inwardly concave surface of the robotic arm it is difficult to realize three-dimensional (3D) braiding directly, and based on this problem, an equivalent convex mandrel is added to the concave mandrel. The mathematical model of the concave mandrel is established, and the mathematical expression of the concave mandrel is obtained. In any cross-section perpendicular to the x -axis, the equivalent outwardly convex cross-sectional line is obtained according to the condition that the length of the outwardly convex and concave mandrel cross-sectional line is equal. All outwardly convex cross-sectional lines form a smooth surface of the equivalent convex mandrel. By the numerical calculation method, the braiding trajectory and the downward pressure trajectory are predicted for various take-up speeds. The length error of the braiding trajectory and the downward pressure trajectory are within 5%, which verifies the accuracy of the convex mandrel. The variation pattern of the braiding angle is smooth, verifying the braidability of the convex mandrel. The experimental results show that the outwardly convex carbon fiber fabric can be pressed down to fit the surface of the concave mandrel. Therefore, a carbon fiber composite arm with an inwardly concave surface can be manufactured by 3D braiding with the addition of an equivalent convex mandrel. [ABSTRACT FROM AUTHOR]

Polylactic acid (PLA) surgical suture is considered to be one of the most ideal materials for tissue closure due to its rich raw materials and excellent biological properties. However, surgical sutures face great challenges due to problems such as wound infection and tissue reaction in practical applications. In order to improve the clinical applicability of surgical sutures, we constructed a new drug-loading system for core-spun surgical sutures. The shell was composed of nanofibrous membranes composed of polyglycolic acid (PGA) and polycaprolactone (PCL) and ciprofloxacin (CIP) antibacterial drugs, and the core layer adopts PLA filament. By adjusting the composition ratio of PGA and PCL in the shell, a new mode of regulating the release rate and release cycle of the suture was constructed. According to different wound healing time, different drug release cycles of surgical suture were selected. In the study, the structure of the core-spun yarn can be clearly observed by scanning electron microscope, the higher the shell PGA content and drug loading, the faster the drug release rate. When the carrier ratio PGA/PCL was 80/20 and the drug loading was 3%, the drug release rate was the fastest and the drug release was high; finally, antibacterial experiments showed that the suture had excellent antibacterial effect and could effectively kill Staphylococcus aureus and Escherichia coli. The successful preparation of core-spun yarn surgical suture provides a new idea for the study of new antibacterial surgical suture. [ABSTRACT FROM AUTHOR]

This paper presented the composite antibacterial agent of nano-silver (Ag) and tea polyphenol (TP) by bioreduction method. Then polylactic acid (PLA) nanofiber membranes with different antibacterial content were prepared by electrostatic spinning method. The morphologies, structural characteristics and properties of electrostatic spun fiber membranes were investigated by scanning electron microscope (SEM), infrared spectroscopy (FTIR), electronic single fiber strength machine and flat colony counting method. The results showed that Ag@TP was loaded with silver nanoparticles with uniform particle size distribution. When the content of Ag@TP of electrospun fiber was less than 2.0%, the diameter of electrostatic spinning fiber was uniformly distributed and had smooth surface. It could be seen from the FTIR spectrum that Ag@TP and PLA were well compounded through valence bonds. As the content of antibacterial agent increased, the elongation at break of the fiber membrane first decreased and then increased. While the breaking strength was found to first decrease, then increase and decrease again. The water contact angle of the fiber membrane was gradually decreased with the increase of the antibacterial agent content. When the amount of Ag@TP was added 2.0%, the relative antibacterial rate of the electrospun fiber membrane to E.coli and S.aureus was as high as 95%, indicating an excellent antibacterial effect. [ABSTRACT FROM AUTHOR]

Microwave absorbers with ultra-broad bandwidths are extremely effective in combating the wide range of electromagnetic pollution and radar detection. However, the conventional magnetoelectric compositing techniques for achieving a wide absorption bandwidth suffer from drawbacks of a complex preparation process and low yield production. A single-phased microwave absorber is an ideal choice for large-scale application while integrating desired levels of dielectric loss and a broad magnetic loss continues to pose a significant challenge. In this work, an ultra-broad single-phase microwave absorber is developed by abating the doping competition of dual high-valence Zr4+ and Ti4+ ions in the M-type barium ferrite. Due to the disparities in electronegativity and ionic radii of Zr4+ and Ti4+ ions, enhanced incorporation of high-valence ions substituting for Fe3+ ions in Zr4+–Ti4+ ion co-doped systems to simultaneously improve the complex permittivity and generate multi-resonance permeability. First-principles calculations and magnetic resonance peak-differentiating and imitating results show that the enhanced electromagnetic properties stem from the optimized electronic structure and strengthened exchange coupling effect. By optimizing the dopant Zr4+ and Ti4+ contents in terms of composition of BaFe12−2xZrxTixO19 with x = 0.4, an ultra-broad bandwidth of 16.3+ GHz and an ultra-strong absorption intensity of up to −54.8 dB have been successfully achieved at a very thin matching thickness down to ∼1.08 mm, much superior to those of the existing various types of state-of-the-art microwave absorbers. Therefore, this material shows great application potentials as preeminent microwave absorbers with unprecedented comprehensive performance. [ABSTRACT FROM AUTHOR]

This article discusses a study that explores the potential use of 3′t‐2′Ome miRNAs as diagnostic biomarkers for non-small cell lung cancer (NSCLC). The study analyzed 149 NSCLC patients and 146 control subjects to evaluate the diagnostic value of these miRNAs in plasma. The analysis found significant differences in underlying diseases between the cancer patients and control subjects, suggesting that 3′t‐2′Ome miRNAs may be useful in diagnosing NSCLC. The article also discusses the use of deep sequencing technology to study small RNA species and the identification of three methylated miRNAs that could serve as biomarkers for early detection of NSCLC. Further research is needed to explore the clinical value and functions of these methylated miRNAs. [Extracted from the article]

Background and Objectives: Obstructive sleep apnea (OSA) is an independent risk factor for stroke. Furthermore, intracranial arterial calcification (IAC) has been validated as a marker for subclinical cerebrovascular disease. However, the relationship between OSA with IAC was less studied compared with its established association with coronary artery calcification. In this study, we aimed to investigate the association between the severity of OSA and the degree of IAC in hospitalized patients without preexisting cardiovascular disease. Methods: This hospital-based observational study was conducted from June 1, 2017, to May 1, 2019. In total, 901 consecutive patients who underwent head computed tomography scans and portable sleep monitoring were included. On the basis of the apnea-hypopnea index (AHI), patients were divided into four OSA severity groups (normal: AHI <5/h; mild: 5≤ AHI <15/h; moderate: 15≤ AHI <30/h; severe: AHI ≥30/h). Associations of OSA with IAC scores were assessed by using multivariate logistic regression analysis. Results: Of the 901 patients, 484 (53.7%) were men; the mean (SD) age was 66.1 (10.0) years. The non-OSA group included 207 (23.0%) patients; mild OSA, 209 (23.2%); moderate OSA, 235 (26.1%); and severe OSA, 169 (18.8%). Mean IAC scores were higher in the severe OSA group compared with non-, mild, and moderate OSA groups (4.79 vs. 2.58; 4.79 vs. 2.94; 4.79 vs. 3.39; p < 0.001). Multivariate analysis adjusted for confounding factors revealed that only severe OSA was associated with a higher IAC score (odds ratio [OR]: 1.65; 95% confidence interval [CI]: 1.43–1.91; p < 0.001). In stratified analyses by BMI, among participants with a BMI <25 kg/m2, the positive association between AHI values and IAC scores was found in the moderate OSA group (OR: 1.23; 95% CI: 1.05, 1.43; p = 0.01) and the severe OSA group (OR: 1.96; 95% CI: 1.55, 2.48; p < 0.001). When stratified by gender, in women, the positive association was found in the moderate OSA group (adjusted OR: 1.21; 95% CI: 1.02–1.51; p = 0.016) and the severe OSA group (adjusted OR: 1.76; 95% CI: 1.36–2.25; p < 0.001). For the men group, a positive association between IAC scores and AHI was only observed in the severe OSA group. Discussion: These findings suggest that OSA, in particular severe OSA (AHI ≥30), is independently associated with higher IAC scores. Women and no-obesity individuals appeared more susceptible to adverse OSA-related subclinical cerebrovascular disease as measured by IAC scores. [ABSTRACT FROM AUTHOR]

Soil organic carbon (SOC) is an important component of the ecosystem carbon pool, and fire is one of the important disturbances in forest ecosystems. With global warming, there has been a gradual increase in boreal forest fires, which has a nonnegligible impact on the SOC dynamics in forests. The CENTURY model was employed in our study to simulate the changes in SOC stocks in boreal forests of the Great Xing'an Mountains, China under different fire severity conditions. Fire severity was represented by the metric of difference normalized burn ratio (dNBR) derived from 30-m Landsat-8 imageries. Changes in forest SOC stocks following fire disturbance were predicted under four future Representative Concentration Pathway (RCP) scenarios (RCP2.6, RCP4.5, RCP6.0, and RCP8.5). We found that the CENTURY model had good results in simulating the SOC stocks in the postfire of China's boreal forests. Forest SOC dynamics responded differently to fire severities and the larger SOC loss was associated with increasing fire severity. Importantly, a feedback mechanism was found between climate change and SOC stocks, which reduces SOC stocks with increasing temperatures. High-severity forest fires tended to cause serious damage to the SOC pool and delay forest SOC recovery time; after such events, forest SOC stocks cannot be fully recovered to the prefire levels (6.74% loss). In addition, higher CO2 emissions and warmer temperatures significantly affected the recovery of SOC stocks after fire disturbance, resulting in larger SOC losses. Overall, we projected losses of 10.14%, 12.06%, 12.41%, and 15.70% of SOC stocks after high-severity fires in four RCP scenarios, respectively. Our findings emphasize the importance of fire disturbance and climate change on future dynamics of SOC stocks in China's boreal forests, providing a scientific basis for future boreal forest management and fire management. [ABSTRACT FROM AUTHOR]

Polyether-based acryloyl-functional acceptor binders were combined with malonated polyester donor resins in base-catalyzed carbon-Michael addition curable paint systems. The high equivalent weight, flexible polyether-based acceptors afford a multi-phase morphology in the final coating film, which is formed through polymerization-induced incompatibility and phase separation. Additional thermal transitions were observed in dynamic mechanical thermal analysis experiments, indicating the presence of low Tg domains in the highly crosslinked continuous phase. As a consequence, these novel network compositions show strongly improved ductility and adhesion robustness over a range of substrate types. Non-reactive polyether-based binders, lacking covalent bonding to the crosslinked continuous phase, only lead to limited, less robust property improvement. It was demonstrated that acceptor binders based on poly(propylene glycol) segments are highly effective in enhancing flexibility while maintaining a high coating hardness. Both clearcoat and pigmented topcoat formulations were developed showing similar performance improvements, opening the door to benefits in a variety of application fields. [ABSTRACT FROM AUTHOR]

Exploring efficient strategies to achieve novel high‐efficiency catalysts for water splitting is of great significance to develop hydrogen energy technology. Herein, unique molybdenum (Mo)‐doped ruthenium–cobalt oxide (Mo–RuCoOx) nanosheet arrays are prepared as a high‐performance bifunctional electrocatalyst toward hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) through combining electronic and vacancy engineering. Theoretical calculations and experimental results reveal that the incorporation of Ru and Mo can effectively tune the electronic structure, and the controllable Mo dissolution coupling with the oxygen vacancy generation during surface reconstruction is able to optimize the adsorption energy of hydrogen/oxygen intermediates, thus greatly accelerating the kinetics for both HER and OER. As a result, the Mo–RuCoOx nanoarrays exhibit remarkably low overpotentials of 41 and 156 mV at 10 mA cm−2 for HER and OER in 1 m KOH, respectively. Furthermore, the two‐electrode electrolyzer assembled by the Mo–RuCoOx nanoarrays requires a cell voltage as low as 1.457 V to achieve 10 mA cm−2 for alkaline overall water splitting. This work holds great promise to develop novel and highly active electrocatalysts for future energy conversion applications. [ABSTRACT FROM AUTHOR]

Global climate change is one of the major challenges facing the world, and the spatial optimization of land use patterns has been regarded as critical in realizing carbon mitigation. In this study, the linear programming model and the Markov Chain model are integrated in different scenarios to optimize land use structure for low-carbon development. The land use pattern is then simulated through the adjusted convolutional neural network and cellular automata model, taking Guangzhou City as the case study area. The results reveal that construction land with high economic efficiency will increase its area, and the reaming types will experience slight changes, in 2035 in the natural development scenario and the economic priority scenario. Ecological land such as forest land, grassland, and water is partly occupied by construction land in the urban–rural fringe areas. The total carbon emissions decrease by 2.32% and 1.57% in these two scenarios. In the low-carbon-oriented scenario, the expansion of construction land is restricted, and the forest land and grassland undergo great expansion. The total carbon emission decreases by 18.95%—a figure much larger than that in the natural development scenario and the economic priority scenario. Our paper embeds the needs and constraints in land spatial planning into the spatial optimization of the land use pattern, which provides valuable references for low-carbon city development in the future. [ABSTRACT FROM AUTHOR]

Remotely operated vehicles (ROVs) and unmanned aerial vehicles (UAVs) provide a solution for dam and bridges structural health information acquisition, but problems like effective damage-related information extraction also occur. Vision-based crack detection methods can replace traditional manual inspection and achieve fast and accurate crack detection. This paper thereby proposes a lightweight, real-time, pixel-level crack detection method based on an improved instance segmentation model. A lightweight backbone and a novel efficient prototype mask branch are proposed to decrease the complexity of the model and maintain the accuracy of the model. The proposed method attains an accuracy of 0.945 at 129 frames per second (FPS). Moreover, our model has smaller volume, lower computational requirements and is suitable for low-performance devices. [ABSTRACT FROM AUTHOR]

With the rapid development of perovskite solar cells, organic–inorganic hybrid Pb–Sn perovskite solar cells have attracted more and more attention in recent years due to their low toxicity, narrow bandgap and advantages of tandem solar cells. But for the present Pb–Sn mixed perovskite, the open circuit voltage is still at a relatively low level. Herein, phenyl–ethyl ammonium bromide (PEABr) was introduced into the perovskite structure by the method of composition regulation, and high quality and stable Pb–Sn perovskite MA0.5FA(0.5-x)PEAxPb0.5Sn0.5I(3-x)Brx films and devices were generated. Compared with the Pb–Sn mixed perovskite without component control treatment, the band structure of perovskite optimized by PEABr changes and the transport of charge carriers is promoted due to the introduction of PEA+ and Br−. In addition, the decomposition of perovskite caused by water and oxygen can be effectively slowed down due to the high stability and hydrophobic characteristics of the two-dimensional material PEA+. Finally, the efficiency of the Pb–Sn mixed perovskite device regulated by PEABr increased from 14.2% to 16.1%, and it could be maintained in the nitrogen environment for 14 days without significant efficiency attenuation, while the efficiency of the perovskite device without PEABr regulation decreased significantly. " [ABSTRACT FROM AUTHOR]

Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) has been identified in various water environments, posing a serious risk to public health. However, whether and how ESBL-producing genes in water-derived E. coli can spread among mammalian gut microbiota via drinking water is largely unclear. To address this problem, horizontal transfer characterization of ESBL-producing genes in mice gut microbiota was determined after the oral ingestion of contaminated water by ESBL-producing E. coli, and then the driving factors were comprehensively examined from multiple different perspectives. The results showed that water-borne ESBL-producing E. coli can colonize in the mice intestine, the ESBL-producing genes can horizontally spread among gut microbiota, and the recipient bacteria include opportunistic pathogens Klebsiella pneumoniae and Salmonella enterica. This horizontal spread may be attributed to the intestinal micro-environment changes caused by the ingestion of contaminated water by ESBL-producing E. coli. These changes, including gut microbiota diversity, increased levels of inflammatory response and reactive oxygen species, cell membrane permeability, and expression levels of conjugative transfer-related genes, are all major driving factors for horizontal transfer of ESBL-producing genes in mice gut microbiota. Our findings highlight the potential for ESBL-producing E. coli to spread resistance genes to mammalian gut microbiota during ingestion of contaminated water. [ABSTRACT FROM AUTHOR]

Social isolation (ISO) is associated with an increased risk and poor outcomes of ischemic stroke. However, the roles and mechanisms of ISO in stroke-associated pneumonia (SAP) remain unclear. Adult male mice were single- or pair-housed with an ovariectomized female mouse and then subjected to transient middle cerebral artery occlusion. Isolated mice were treated with the natriuretic peptide receptor A antagonist A71915 or anti-gamma-delta (γδ) TCR monoclonal antibody, whereas pair-housed mice were treated with recombinant human atrial natriuretic peptide (rhANP). Subdiaphragmatic vagotomy (SDV) was performed 14 days before single- or pair-housed conditions. We found that ISO significantly worsened brain and lung injuries relative to pair housing, which was partially mediated by elevated interleukin (IL)-17A levels and the migration of small intestine-derived inflammatory γδ T-cells into the brain and lung. However, rhANP treatment or SDV could ameliorate ISO-exacerbated post-stroke brain and lung damage by reducing IL-17A levels and inhibiting the migration of inflammatory γδ T-cells into the brain and lung. Our results suggest that rhANP mitigated ISO-induced exacerbation of SAP and ischemic cerebral injury by inhibiting small intestine-derived γδ T-cell migration into the lung and brain, which could be mediated by the subdiaphragmatic vagus nerve. [ABSTRACT FROM AUTHOR]