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An electrochemical sensor was developed for the detection of hydrogen peroxide (H2O2), utilizing the synergistic effects of graphene (Gr) and MOF-on-MOF nanozymes (FeCu-NZs). Initially, Fe-MOF with peroxide-like activity is synthesized using a solvothermal method. Subsequently, the organic ligand on its surface binds Cu2+, enhancing the enzyme-like activity further. The resulting FeCu-NZs exhibit a distinctive electrochemical signal in response to H2O2. Moreover, integrating FeCu-NZs with Gr significantly amplifies the electrochemical signal and effectively reduces the sensor's detection limit. The developed sensor exhibited linear ranges of 0.1–3800 μM, with a limit of detection (LOD) of 0.06 μM. Additionally, FeCu-NZs catalyze H2O2 to generate abundant •OH radicals, and colorimetric detection of H2O2 is facilitated using the color rendering principle of 3,3',5,5'-tetramethylbenzidine (TMB). Notably, this detection method was applied to determine H2O2 concentrations in real samples, achieving a recovery exceeding 95.7%. In summary, this research provides a practical platform for the construction of traditional nanozymes and the integration of electrochemical systems, which have broad applications in food analysis, environmental monitoring, and medical diagnosis. [ABSTRACT FROM AUTHOR]

Sustainability is an important topic in the development process of urban agglomerations. Sustainability assessment is an effective way to monitor the sustainable development of urban agglomerations. Based on the three‐pillars model, the paper assessed the sustainability of the three major urban agglomerations in China by selecting 18 indicators from economic, social, and environmental dimensions. A new sustainability‐differentiation‐combined weighting method was proposed, which considers the sustainability at indicator level as well as highlights the difference at urban agglomeration level. The assessment results show that the Pearl River Delta (PRD) urban agglomeration performed best, followed by the Yangtze River Delta (YRD) urban agglomeration and the Beijing‐Tianjin‐Hebei (BTH) urban agglomeration. However, the sustainability level of the three urban agglomerations was not high. It primarily caused by the downward development trend of economic sustainability of the three urban agglomerations. And the YRD and PRD urban agglomerations had lower performances on social sustainability. The spatial sustainability of the urban agglomerations was also analyzed, and the results show the spatial linkage development within the urban agglomerations was poor. Based on these findings, some valuable suggestions were provided as reference for the sustainability construction of the urban agglomerations. [ABSTRACT FROM AUTHOR]

Background: Non-SMC condensin I complex subunit G (NCAPG), a member of the subunit of condensin complex, is significantly overexpressed in various cancers and involved in the pathogenesis of cancers. However, the roles of NCAPG in ovarian cancer remain unclear. Methods: The mRNA expression, overall survival, and disease-free survival of NCAPG in ovarian cancer were analyzed by GEPIA and KM plotter database, and the expression levels of NCAPG in OC tissues and cell lines were determined by qPCR and immunohistochemistry analysis. shRNA targeting NCAPG gene (sh-NCAPG) was utilized to knock down NCAPG expression in OVCAR3 and SKOV3 cells. Subsequently, CCK-8 assay, colony formation assay, transwell invasion assay and flow cytometric analysis were performed to detect the effect of NCAPG on OC cell proliferation, apoptosis, and invasion. Finally, western blot assays were performed to detect the mechanism of NCAPG in ovarian cancer. Results: Analysis using GEPIA and KM plotter database showed NCAPG was upregulated in ovarian cancer and negatively associated with the survival of OC patients. qPCR and immunohistochemistry analysis confirmed it was highly expressed in both ovarian cancer tissues and cells. The silencing of NCAPG inhibited OC cell proliferation and invasion, and induced cell apoptosis. Additionally, flow cytometric analysis revealed that NCAPG knockdown arrested the cell cycle at G2 and S phases. Furthermore, we also found that downregulation of NCAPG could suppress OC cell proliferation and invasion via activating the p38 MAPK signaling pathway. Conclusion: Our results suggest that NCAPG exhibits an important role in the development and progression of ovarian cancer and implicates NCAPG as a potential therapeutic target in ovarian cancer. [ABSTRACT FROM AUTHOR]

Aim: This study was intended to identify potentially target genes and underlying biological pathway of sanguinarine in ovarian cancer. Methods: We obtained the expression changes of downstream target genes and underlying biological pathways regulated by control and sanguinarine groups via Affymetrix gene expression profile chip in ovarian cancer cells. An Affymetrix Genechip Agilent mRNA Array was used to recognize differentially expressed genes (DEGs). Afterward, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed for the DEGs using the DAVID database. Results: A total of 1185 DEGs between sanguinarine and control groups were identified, including 835 upregulated and 350 downregulated DEGs. The result of GO analysis recommended that the DEGs were mostly enriched in biological processes, including negative regulation of gene expression, nitrogen compound metabolic process, and transcription from RNA polymerase II promoter. Alterations in cellular components (CC) were suggestively enriched in the cytoskeleton and endoplasmic reticulum. The changes in molecular function were suggestively enriched in nucleic acid-binding transcription factor activity, protein dimerization activity, and enzyme binding. The results of the KEGG pathway analysis indicated that the DEGs were mostly concentrated in "Systemic lupus erythematosus," "MAPK signalling pathway," "Pathways in cancer," and "Metabolic pathways." Conclusion: The present study provided insights into the mechanism underlying sanguinarine target genes in ovarian cancer cells, which might be used as effective targets for OC diagnosis and treatment. [ABSTRACT FROM AUTHOR]

Spectral methods have previously been applied to analyze a multitude of vibration and acoustic problems due to their high computational efficiency. However, their application to interior structural acoustics systems has been limited to the analysis of a single plate coupled to a fluid-filled cavity. In this work, a general multidomain spectral approach is proposed for the eigenvalue and steady-state vibroacoustic analyses of interior structural-acoustic problems with discontinuous boundaries. The unified formulation is derived by means of a generalized variational principle in conjunction with the spectral discretization procedure. The established framework enables one to easily accommodate complex systems consisting of both a structure assembly and a built-up cavity with moderate geometric complexities and to effectively analyze vibroacoustic behaviors with sufficient accuracy at relatively high frequencies. Two practical examples are chosen to demonstrate the flexibility and efficiency of the proposed formulation: a built-up cavity backed by an assembly of multiple connected plates with arbitrary orientations and a thick irregular elastic solid coupled with a heavy acoustic medium. Comparison to finite element simulations and convergence studies for these two examples illustrate the considerable computational advantage of the method as compared to finite element procedures. [ABSTRACT FROM AUTHOR]

Cell‐derived microparticles, which are recognized as nanosized phospholipid bilayer membrane vesicles, have exhibited great potential to serve as drug delivery systems in cancer therapy. However, for the purpose of comprehensive therapy, microparticles decorated with multiple therapeutic components are needed, but effective engineering strategies are limited and still remain enormous challenges. Herein, Bi2Se3 nanodots and doxorubicin hydrochloride (DOX) co‐embedded tumor cell‐derived microparticles (Bi2Se3/DOX@MPs) are successfully constructed through ultraviolet light irradiation‐induced budding of parent cells which are preloaded with Bi2Se3 nanodots and DOX via electroporation. The multifunctional microparticles are obtained with high controllability and drug‐loading capacity without unfavorable membrane surface destruction, maintaining their excellent intrinsic biological behaviors. Through membrane fusion cellular internalization, Bi2Se3/DOX@MPs show enhanced cellular internalization and deepened tumor penetration, resulting in extreme cell damage in vitro without considering endosomal escape. Because of their distinguished photothermal performance and tumor homing target capability, Bi2Se3/DOX@MPs exhibit admirable dual‐modal imaging capacity and outstanding tumor suppression effect. Under 808 nm laser irradiation, intravenous injection of Bi2Se3/DOX@MPs into H22 tumor‐bearing mice results in remarkably synergistic antitumor efficacy by combining photothermal therapy with low‐dose chemotherapy in vivo. Furthermore, the negligible hemolytic activity, considerable metabolizability, and low systemic toxicity of Bi2Se3/DOX@MPs imply their distinguished biocompatibility and great potential for tumor theranostics. [ABSTRACT FROM AUTHOR]

Aim: The extent and specifics regarding cognitive dysfunction in patients with bipolar disorder (BD) or major depressive disorder (MDD) and their unaffected first‐degree relatives (FDR) have not been addressed in any single study. The present study compared the cognitive function of patients with BD or MDD, their FDR, and healthy control (HC) individuals. Methods: The study population comprised adults (aged 18–55 years) with BD, adults with MDD, FDR (children or siblings of patients with BD or MDD), and HC (n = 105, 109, 85, and 95, respectively). The Repeatable Battery for the Assessment of Neuropsychological Status was used to assess neurocognitive functions, with five domains and 12 tests. A Wechsler Adult Intelligence Scale brief form was applied to evaluate IQ. Status of mood was assessed using the Young Mania Rating Scale and the Hamilton Depression Scale. Results: The mixed model indicated significant variation among the four groups in cognitive function. Cognitive impairments, compared to HC, progressively greater from least to most were found in: FDR, MDD, and BD (F = 32.74, P < 0.001). Years of education correlated with cognitive performance (F = 17.04, P < 0.001), as did IQ (F = 240.63, P < 0.001). The total score for the Hamilton Rating Scale for Depression negatively correlated with cognitive function (F = 5.78, P = 0.017). Conclusion: Among the study groups, patients with BD had the most severe deficits, followed by MDD patients and FDR. Cognitive deficits could not be associated with a specific psychiatric disorder, but differences in degree were noted. [ABSTRACT FROM AUTHOR]