1. Comparison of combined antioxidants and thymoquinone in the prevention of testis ischemia - reperfusion injury.
- Author
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Erol, B., Sari, U., Amasyali, A. S., Ozkanli, S., Sogut, S., Hanci, V., Efiloglu, O., Danacioglu, Y. O., Engin, P., Yencilek, F., Atis, G., Yildirim, A., Alkoc, O. A., and Caskurlu, T.
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ISCHEMIA ,REPERFUSION ,TESTIS ,MALONDIALDEHYDE ,OXIDANT status - Abstract
We aimed to compare the preventive effects of combined antioxidants ( CA1, 2) with a single antioxidant drug (thymoquinone; TQ) on experimental testis Ischemia/Reperfusion (I/R) injury. Thirty-five adult male Wistar rats were divided into five groups of seven rats each: control, testis I/R, testis I/R + CA1, testis I/R + CA2, and testis I/R + TQ. After 1 h of testicular ischemia, reperfusion was achieved by detorsion for 4 h. Antioxidants were intraperitoneally administered for 30 min prior to reperfusion. All rats were sacrificed 4 h after reperfusion to evaluate the tissue levels of malondialdehyde ( MDA) and total antioxidant status ( TAS) and the immunohistochemical evaluation of tissue inducible and endothelial nitric acid synthase ( iNOS, eNOS) and apoptosis protease-activating factor 1 ( APAF-1). MDA levels were lower and TAS values were higher in the I/R + antioxidant groups than in the I/R group ( p < 0.05). iNOS and eNOS levels in the I/R + antioxidant groups were also lower than those in the I/R group ( p < 0.05). There were no significant differences between the CA groups and the TQ group according to aforementioned parameters. In addition, tissue APAF-1 values were significantly higher in the I/R group than in the other groups. However, there was a significant difference between the TQ and CA groups in APAF-1 levels, which were highest in the TQ group ( p < 0.05). Although TQ alone increased TAS values and reduced tissue iNOS and eNOS levels, combined antioxidant treatment may more effectively reduce apoptosis and increase preventive effects in testis I/R injury. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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