32 results on '"Xing Shao"'
Search Results
2. The Influence of Triphenyltin Exposure on the Osmoregulatory Capacity of Marine Medaka (Oryzias melastigma) at Different Salinities.
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Li, Teng-Zhou, Chen, Cheng-Zhuang, Xing, Shao-Ying, Liu, Ling, Li, Ping, and Li, Zhi-Hua
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ORYZIAS latipes ,OSMOTIC pressure ,SALINITY ,OSMOREGULATION - Abstract
Triphenyltin (TPT) is an organotin pollutant widely found in the aquatic environment. It has endocrine-disrupting and osmotic pressure toxicity. In this study, the physiological and biochemical effects of TPT and various salinities were investigated in different tissues (gut, gill, and brain) of marine medaka. The exposure experiments were conducted for 42 days in different salinities (0, 15, and 30 ppt) without TPT exposure and in different salinity groups with TPT exposure concentrations of 100 ng/L, respectively. The results showed that the Na
+ -K+ -ATPase (NKA) and Ca2+ ATPase activity had significant tissue-specific differences, with the highest activity observed in the gills, indicating their major contribution to osmoregulation. Changes in salinity also resulted in significant alterations in the ion concentrations of the gut and gill tissues in the 0-C and 15-C groups. While the changes in Na+ and Cl− were relatively stable, the presence of TPT disrupted the regulation of Ca2+ and K+ . In conclusion, substantial variations were observed in the osmoregulatory capacity of marine medaka tissues. Environmental concentrations of TPT had little effect on osmotic enzyme activity but interfered with the regulation of Ca2+ and K+ concentrations in the tissues. This study provides valuable insights into the osmotic toxicity of TPT in aquatic environments with different salinities. [ABSTRACT FROM AUTHOR]- Published
- 2024
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3. Bearing fault diagnosis method based on improved deep residual Siamese neural network.
- Author
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Chen Qian, Jun Gao, Xing Shao, Cuixiang Wang, and Jianhua Yuan
- Subjects
FAULT diagnosis ,DIAGNOSIS methods ,ROLLER bearings ,EUCLIDEAN distance ,FEATURE extraction ,DEEP brain stimulation - Abstract
Aiming to address the problem that faults in rolling bearings make effective fault diagnosis difficult under small-sample and varying working conditions, this paper proposes a new fault diagnosis method for rolling bearings that monitors their vibration signals and is based on an improved deep residual Siamese neural network, called a WDRCNN. Firstly, the Siamese neural network is applied to extract features with shared weights to achieve an expansion in the number of fault samples. Then, multiple residual blocks are used to extract deeper feature information and effectively alleviate the problem of overfitting. In addition, the attention mechanism is employed to assign weights to the feature information to reduce the interference of redundant features. Finally, the Euclidean distance between the sample pairs is calculated to determine the similarity of the sample pairs and to classify bearing faults for end-to-end bearing fault diagnosis. The experimental results demonstrate that the WDRCNN achieves an average accuracy of 96.31% under different operating conditions. Even when only 90 training samples are available, the WDRCNN achieves an accuracy of over 93%. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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4. Structure design and hydraulic performance analysis of drip emitters based on vortex energy dissipation.
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Wang, Yue, Liu, Ning‐ning, Wang, Zhen‐hua, Tan, Ming‐dong, Xu, Xin, and Xing, Shao‐bo
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ENERGY dissipation ,MICROIRRIGATION ,SEDIMENTATION & deposition ,HYDRAULIC structures ,PROBLEM solving ,STRUCTURAL optimization - Abstract
Copyright of Irrigation & Drainage is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2024
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5. Design, synthesis, and biological evaluation of thiazole/thiadiazole carboxamide scaffold-based derivatives as potential c-Met kinase inhibitors for cancer treatment.
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Nan, Xiang, Wang, Qiu-Xu, Xing, Shao-Jun, and Liang, Zhi-Gang
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THIAZOLES ,KINASE inhibitors ,CARBOXAMIDES ,AMIDE derivatives ,CANCER treatment ,STRUCTURE-activity relationships ,CELL cycle - Abstract
As part of our continuous efforts to discover novel c-Met inhibitors as antitumor agents, four series of thiazole/thiadiazole carboxamide-derived analogues were designed, synthesised, and evaluated for the in vitro activity against c-Met and four human cancer cell lines. After five cycles of optimisation on structure–activity relationship, compound 51am was found to be the most promising inhibitor in both biochemical and cellular assays. Moreover, 51am exhibited potency against several c-Met mutants. Mechanistically, 51am not only induced cell cycle arrest and apoptosis in MKN-45 cells but also inhibited c-Met phosphorylation in the cell and cell-free systems. It also exhibited a good pharmacokinetic profile in BALB/c mice. Furthermore, the binding mode of 51am with both c-Met and VEGFR-2 provided novel insights for the discovery of selective c-Met inhibitors. Taken together, these results indicate that 51am could be an antitumor candidate meriting further development. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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6. Evaluation of Physiological Stress of Grass Carp Chronically Exposed to Enrofloxacin Based on IBR Index.
- Author
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Cao, Xu-Qian, Wang, Xu, Liu, Bin, He, Shu-Wen, Cao, Zhi-Han, Xing, Shao-Ying, Li, Ping, and Li, Zhi-Hua
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CTENOPHARYNGODON idella ,PHYSIOLOGICAL stress ,DIGESTIVE enzymes ,ACETYLCHOLINESTERASE ,POISONS ,FLUOROQUINOLONES ,SUPEROXIDE dismutase - Abstract
Enrofloxacin (ENR) is a commonly used drug in aquaculture, and it is frequently detected in the aquatic environment. Data on ENR toxicity toward aquatic species are limited. This study was aimed at using different biomarkers to evaluate the possible toxic effects of grass carp (Ctenopharyngodon idella) exposed to 0 (control), 1, 100, and 10,000 μg/L enrofloxacin for 21 days as a sub-chronic exposure trial, oxidative stress biomarkers (including superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and malondialdehyde (MDA)), neurotoxicity indicators (including acetylcholinesterase (ACHE) activity, nitric oxide (NO)), and digestive enzyme activities (including lipase (LPS), amylase (AMS) enzymes). In addition, an integrated biomarker response (IBR) index was utilized to evaluate the integrated toxic effects of ENR on grass carp. Our results demonstrated that ENR exposure significantly increased activities of CAT, LPS, and AMS. ENR exposure also significantly upregulated the expression levels of sod1, ACHE, LPL, ATGL, and AMY genes. Furthermore, histopathological changes were observed in the hepatopancreatic tissues of grass carp exposed to ENR. It was observed that higher IBR scores were noticed in the tissues of fish exposed to ENR, suggesting an induced biological response. The comprehensive biomarker index showed that CAT and ACHE activities have a higher response to ENR, and 100 μg/L has a greater impact on grass carp. These results indicate that ENR has a toxic effect on grass carp and impairs their physiological functions. This is the first study to explore the effects of ENR on grass carp, and it provides basic information for assessment of ENR effects in aquaculture. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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7. Combined experimental and simulation study on corrosion behavior of B10 copper–nickel alloy welded joint under local turbulence.
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Zhang, Da-lei, Liu, Ran, Liu, Ying-shuang, Xing, Shao-hua, He, Zong-hao, Qian, Yao, Liu, Jin-zeng, Dou, Xiao-hui, and Zhang, Xin-wei
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- 2023
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8. Effects of long-term exposure of norfloxacin on the HPG and HPT axes in juvenile common carp.
- Author
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Zhang, Si-Qi, Zhao, Xue-Li, He, Shu-Wen, Xing, Shao-Ying, Cao, Zhi-Han, Li, Ping, and Li, Zhi-Hua
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NORFLOXACIN ,CARP ,ENDOCRINE system ,FISH populations ,AQUATIC animals ,SEX hormones ,THYROID hormone receptors - Abstract
Currently, there is a relatively lack of relevant research on the interference effect of quinolone antibiotics on the endocrine of aquatic animals. In this study, the toxicity of norfloxacin (NOR) on the endocrine system of juvenile common carp (Cyprinus carpio) was evaluated, as well as the hematocyte parameters. Specifically, two important endocrine axes were assessed: the hypothalamus–pituitary–thyroid (HPT) axis and hypothalamus–pituitary–gonadal (HPG) axis. Norfloxacin was used as a representative of quinolone antibiotics. According to the concentration of water pollution areas and considering the bad situation that may be caused by wastewater discharge, a control, 100 ng/L NOR, and 1 mg/L NOR treatment groups were set up. The juvenile carp, as the test animal, was subjected to an exposure experiment for 42 days. Thyroid hormones (T3 and T4) and related genes in HPT axis and sex hormones (11-ketotestosterone [11-KT] and progesterone [PROG]) and related genes in HPG axis and blood count are tested. It was found that the T4 iodine level and conversion process were enhanced after NOR treatment, which in turn led to the increase of T3 content and biological activity in the blood. One hundred nanograms per liter NOR can inhibit the level of sex hormones and inhibit the expression of HPG axis-related genes. In the 1 mg/L NOR treatment group, long-term exposure over a certain concentration range may lead to the development of adaptive mechanisms, making the changes in hormones and related genes insignificant. In conclusion, this study provides reference data for the endocrine interference of quinolone antibiotics on aquatic organisms, and has ecological significance for assessing the health of fish populations of quinolone antibiotics. However, the specific sites and mechanisms of action related to the effects of NOR on the endocrine system remain unclear and require further study. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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9. Interaction between local corrosion and flow field of natural gas long-distance pipeline by artificial rectangular defect pit.
- Author
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Tan, Zhuo-wei, Wang, Zhen-bo, Bai, Shu-yu, Zhang, Da-lei, Zhang, Sheng-zhu, Cheng, Frank, Xing, Shao-hua, and Jin, You-hai
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- 2022
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10. Treatment of Reducible Atlantoaxial Dislocation and Basilar Invagination Using the Head Frame Reduction Technique and Atlantoaxial Arthrodesis.
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Li, Teng, Du, Yue-Qi, Yin, Yi-Heng, Xing, Shao-Ling, and Qiao, Guang-Yu
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- 2022
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11. A Mini-review of the Toxicity of Pollutants to Fish Under Different Salinities.
- Author
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Xing, Shao-Ying, Li, Zhi-Hua, Li, Ping, and You, Hong
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ECOLOGICAL risk assessment ,POLLUTANTS ,ESTUARIES ,COASTAL organisms ,FISH development ,SALINITY ,CHRONIC toxicity testing - Abstract
In recent years, with the development of the global economy, water pollution has increased. Pollutants migrate, accumulate, and diffuse in aquatic environments. Most of the pollutants eventually enter aquatic organisms. The accumulation of pollutants affects the development and reproduction of organisms, and many pollutants have teratogenic, carcinogenic, and/or mutagenic effects. Aquatic organisms in estuaries and coastal areas are under pressure due to both salinity and pollutants. Among them, salinity, as an environmental factor, may affect the behavior of pollutants in the aquatic environment, causing changes in their toxic effects on fishes. Salinity also directly affects the growth and development of fishes. Therefore, this paper focuses on metals and organic pollutants and discusses the toxic effects of pollutants on fish under different salinities. This research is of great significance to environmental protection and ecological risk assessment of aquatic environments. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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12. Identification and Evaluation of Recombinant Outer Membrane Proteins as Vaccine Candidates Against Klebsiella pneumoniae.
- Author
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Zhang, Bao-Zhong, Hu, Danyu, Dou, Ying, Xiong, Lifeng, Wang, Xiaolei, Hu, Jingchu, Xing, Shao-Zhen, Li, Wenjun, Cai, Jian-Piao, Jin, Meiling, Zhang, Mengya, Lin, Qiubin, Li, Min, Yuen, Kwok-Yung, and Huang, Jian-Dong
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MEMBRANE proteins ,KLEBSIELLA pneumoniae ,VACCINE trials ,COMMUNITY-acquired infections ,NOSOCOMIAL infections - Abstract
Klebsiella pneumoniae found in the normal flora of the human oral and intestinal tract mainly causes hospital-acquired infections but can also cause community-acquired infections. To date, most clinical trials of vaccines against K. pneumoniae have ended in failure. Furthermore, no single conserved protein has been identified as an antigen candidate to accelerate vaccine development. In this study, we identified five outer membrane proteins of K. pneumoniae , namely, Kpn_Omp001, Kpn_Omp002, Kpn_Omp003, Kpn_Omp004, and Kpn_Omp005, by using reliable second-generation proteomics and bioinformatics. Mice vaccinated with these five KOMPs elicited significantly higher antigen-specific IgG, IgG1, and IgG2a. However, only Kpn_Omp001, Kpn_Omp002, and Kpn_Omp005 were able to induce a protective immune response with two K. pneumoniae infection models. These protective effects were accompanied by the involvement of different immune responses induced by KOMPs, which included KOMPs-specific IFN-γ-, IL4-, and IL17A-mediated immune responses. These findings indicate that Kpn_Omp001, Kpn_Omp002, and Kpn_Omp005 are three potential Th1, Th2, and Th17 candidate antigens, which could be developed into multivalent and serotype-independent vaccines against K. pneumoniae infection. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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13. A Physical Layer Network Coding Based Tag Anti-Collision Algorithm for RFID System.
- Author
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Cuixiang Wang, Xing Shao, Yifan Meng, and Jun Gao
- Subjects
ALGORITHMS ,RADIO frequency identification systems ,MULTICASTING (Computer networks) ,LINEAR network coding ,SEARCH algorithms ,RADIO frequency ,BINARY codes - Abstract
In RFID (Radio Frequency IDentification) system, when multiple tags are in the operating range of one reader and send their information to the reader simultaneously, the signals of these tags are superimposed in the air, which results in a collision and leads to the degrading of tags identifying efficiency. To improve the multiple tags' identifying efficiency due to collision, a physical layer network coding based binary search tree algorithm (PNBA) is proposed in this paper. PNBA pushes the conflicting signal information of multiple tags into a stack, which is discarded by the traditional anti-collision algorithm. In addition, physical layer network coding is exploited by PNBA to obtain unread tag information through the decoding operation of physical layer network coding using the conflicting information in the stack. Therefore, PNBA reduces the number of interactions between reader and tags, and improves the tags identification efficiency. Theoretical analysis and simulation results using MATLAB demonstrate that PNBA reduces the number of readings, and improve RFID identification efficiency. Especially, when the number of tags to be identified is 100, the average needed reading number of PNBA is 83% lower than the basic binary search tree algorithm, 43% lower than reverse binary search tree algorithm, and its reading efficiency reaches 0.93. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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14. Monomer gypenoside LI from Gynostemma pentaphyllum inhibits cell proliferation and upregulates expression of miR‐128‐3p in melanoma cells.
- Author
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Zu, Ma‐Li, Piao, Xiang‐Lan, Gao, Jia‐Mei, Xing, Shao‐Fang, and Liu, Lin‐Hua
- Subjects
GYNOSTEMMA pentaphyllum ,CELL proliferation ,MELANOMA ,MICROPHTHALMIA-associated transcription factor ,CELL cycle ,MONOMERS - Abstract
Gypenosides have anticancer activity against many cancers. Gypenoside LI is a gypenoside monomer from Gynostemma pentaphyllum, its pharmacological functions in melanoma have not been reported. In this study, we found that gypenoside LI had a potent cytotoxic effect on melanoma cells. Gypenoside LI can induce intrinsic apoptosis along with S phase arrest. Furthermore, gypenoside LI inhibited the colony formation ability of melanoma through inhibition of the Wnt/β‐catenin signaling pathway. Interestingly, we also found that gypenoside LI can induce the upregulation of the tumor suppressor miR‐128‐3p during melanoma apoptosis. In contrast, gypenoside LI induced apoptosis, cell cycle arrest, and inhibition of the Wnt/β‐catenin signaling pathway, which were abolished by overexpression of the miR‐128‐3p inhibitor in A375 cells. Taken together, these results showed that gypenoside LI could inhibit human melanoma cells through inducing apoptosis, arresting cell cycle at the S phase and suppressing the Wnt/β‐catenin signaling pathway in a miR‐128‐3p dependent manner. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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15. Novel dammarane-type saponins from Gynostemma pentaphyllum and their neuroprotective effect.
- Author
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Xing, Shao-Fang, Lin, Man, Wang, Yu-Rong, Chang, Tuo, Cui, Wei-Ye, and Piao, Xiang-Lan
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GYNOSTEMMA pentaphyllum ,MASS spectrometry ,SAPONINS ,OXIDATIVE stress ,CELL survival - Abstract
Three novel dammarane-type saponins, 2α,3β,12β,20(S),24(S)-pentahydroxydammar-25-ene-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-20-O-β-D-glucopyranoside (1, namely gypenoside J1), 2α,3β,12β,20(S),25-pentahydroxydammar-23-ene-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-20-O-β-D-glucopyranoside (2, namely gypenoside J2) and 2α,3β,12β,20(S)-tetrahydroxydammar-25-en-24-one-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-20-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside (3, namely gypenoside J3) along with one known gypenoside (gypenoside LVII) were isolated from the aerial parts of G. pentaphyllum using various chromatographic methods. Their structures were elucidated on the basis of IR, 1D- (
1 H and13 C), 2D-NMR spectroscopy (HSQC, HMBC and COSY), and mass spectrometry (ESI-MS/MS). Their activity was tested using CCK-8 assay. These four compounds showed little anti-cancer activity with IC50 values more than 100 μM against four types of human cancer lines. The effects of them against H2 O2 -induced oxidative stress in human neuroblastoma SH-SY5Y cells were evaluated and they all showed potential neuroprotective effects with 3.64–18.16% higher cell viability than the H2 O2 -induced model group. [ABSTRACT FROM AUTHOR]- Published
- 2020
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16. Common network coding condition and traffic matching supported network coding aware routing for wireless multihop network.
- Author
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Cuixiang Wang, Xing Shao, Zhi Gao, Chuanxin Zhao, and Jun Gao
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LINEAR network coding ,SCHEDULING - Abstract
Network coding–based routing has been widely considered as a promising technology to improve the network throughput of wireless multihop network. However, existing network coding aware routings have the problem of failure decoding and usually neglect the transmission scheduling, which degrades the gain of network coding. In this article, a common network coding condition and traffic matching supported network coding aware routing is proposed for wireless multihop network. In this, common network coding condition is proposed to avoid the problem of failed decoding. In addition, traffic matching mechanism is presented to promote the occurrence of network coding. Besides, a coding and load aware routing metric, which jointly considers the network coding opportunity and node load, is designed to evaluate the discovered paths. Extensive simulations on NS2 demonstrate that CTNR increases the number of coding opportunities and network coding occurrence, and improves network throughput. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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17. Low expression of BEX1 predicts poor prognosis in patients with esophageal squamous cell cancer.
- Author
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Geng, Hai-Tao, Cheng, Zhi-Wen, Cao, Rui-Juan, Wang, Zhen-Bo, Xing, Shao-Zhi, Guo, Chen, Wang, Feng, Liu, Chang-Min, Chen, Shao-Shui, and Cheng, Yu-Feng
- Published
- 2018
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18. Determination of flavonoids from Gynostemma pentaphyllum using ultra-performance liquid chromatography with triple quadrupole tandem mass spectrometry and an evaluation of their antioxidant activity in vitro.
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Wang, Yu-Rong, Xing, Shao-Fang, Lin, Man, Gu, Yu-Long, and Piao, Xiang-Lan
- Subjects
GYNOSTEMMA pentaphyllum ,GYNOSTEMMA ,HERBAL teas ,FLAVONOIDS ,CUCURBITACEAE - Abstract
Gynostemma pentaphyllum, a traditional herbal tea, and its flavonoids possess antioxidant activities. This study was carried out to isolate and identify these flavonoids from G. pentaphyllum and determinate the contents of the main flavonoids and their antioxidant activities. Nine flavonoids were determinated rapidly from G. pentaphyllum using medium-pressure liquid chromatography combined with other simple chromatographic methods. And nine flavonoids were identified as rutin (1), 4′-O-methyl-kaempferol-3-O-rutinoside (2), ombuoside (3), kaempferol-3-β-D-O-rutinoside (4), isorhamnetin-3-O-β-D-rutinoside (5), quercetin-3-O-β-D-glucoside (6), isorhamnetin (7), kaempferol (8), and quercetin (9) by liquid chromatography mass spectrometry-ion trap time of flight and
13 C nuclear magnetic resonance spectroscopy, respectively. All of them displayed various potent antioxidant effects against the DPPH radical and A549 cell injury by using H2 O2 -generate peroxyl radicals in vitro. A sensitive and specific ultra-performance liquid chromatography coupled with multiple-reaction monitoring mode was utilized to rapidly analyze the main six flavonoids of the ethanol extract of G. pentaphyllum within less than 8 min using a Waters Acquity UPLC BEH C18 column. The contents of them were from 57.11 to 12907.74 µg/g in G. pentaphyllum. [ABSTRACT FROM AUTHOR]- Published
- 2018
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19. Inhibitory Effect of Damulin B from Gynostemma pentaphyllum on Human Lung Cancer Cells.
- Author
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Xing, Shao-Fang, Liu, Lin-Hua, Zu, Ma-Li, Lin, Man, Zhai, Xin-Fang, and Piao, Xiang-Lan
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REACTIVE oxygen species ,APOPTOSIS ,BIOLOGICAL transport ,CELL cycle ,CELL lines ,CELL motility ,CYTOPLASM ,FIBROBLASTS ,GENE expression ,GLYCOSIDES ,HEMOPROTEINS ,INTERLEUKINS ,LUNG tumors ,MELONS ,METABOLISM ,METASTASIS ,MITOCHONDRIA ,MOLECULAR structure ,ONCOGENES ,TREATMENT effectiveness ,CASPASES ,SIGNAL peptides ,MATRIX metalloproteinases ,IN vitro studies ,CELL cycle proteins ,THERAPEUTICS - Abstract
Damulin B, a dammarane-type saponin from steamed Gynostemma pentaphyllum , exhibits the strongest activity against human lung carcinoma A549 cells among the isolated active saponins. In this study, the structure-activity relationship of a series of saponin compounds was discussed. The inhibitory effect of damulin B on human lung cancer A549 and H1299 cells was investigated from apoptosis, cell cycle, and migration aspects. In vitro , human lung cancer cells were more susceptible to damulin B treatment than human normal fibroblasts. Damulin B exhibited a strong cytotoxic effect, as evidenced by the increase of apoptosis rate, reduction of mitochondrial membrane potential (MMP), generation of reactive oxygen species, and G0/G1 phase arrest. Furthermore, damulin B activated the following: both intrinsic and extrinsic apoptosis pathways along with early G1 phase arrest via the upregulation of the Bax, Bid, tBid, cleaved caspase-8, and p53 expression levels; downregulation of the procaspase-8/-9, CDK4, CDK6, and cyclin D1 expression levels; and more release of cytochrome c in the cytoplasm. In addition, antimigratory activities and suppressive effects on metastasis-related factors, such as MMP-2 and MMP-9, accompanied by the upregulation of IL-24 were revealed. Altogether, the results proved that damulin B could inhibit human lung cancer cells by inducing apoptosis, blocking the cell cycle at early G0/G1 phase and suppressing the migration. Hence, damulin B has potential therapeutic efficacy against lung cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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20. Modeling and Experimental Study of Mechanical Properties of Confined Concrete Arch in Complicated Deep Underground Engineering.
- Author
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Shucai Li, Qi Wang, Bei Jiang, Manchao He, Huibin Sun, Xing Shao, Chunhe Wang, Qian Qin, and Hengchang Yu
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STRUCTURAL geology ,CONCRETE construction ,MECHANICAL behavior of materials ,PHYSICAL geology ,BEARING capacity (Bridges) - Abstract
Control of surrounding rocks is one of the major problems in deep underground engineering under complicated conditions, such as high stress and tectonic fracture zones. This study proposed a three-dimensional confined concrete support system (CCSS) and investigated its main component: the confined concrete (CC) arch. The authors derived an internal force calculation formula for a circular arch with any number of sections and studied the effects of load, lateral pressure coefficient, node rigidity ratio, and other factors on the internal force distribution. In the analysis of CC part strength, damage first occurred to the CC159_10-C40 arch on both sides of the right and the left casings at an ultimate bearing capacity of 2,210 kN. To verify the correctness of the theoretical calculation and investigate the mechanical properties and damage mechanism of the arch, a full-scale indoor test on the CC159_10-C40 arch was conducted, and a 1:1 large-scale test system was developed. The authors found that the arch ultimate bearing capacity under the same load was 2,003.2 kN, a difference of only 9.36% compared with the theoretical calculation. The arch damage mechanism and key damaged parts were found to be consistent with the predictions of the theoretical calculations. The results of this research provide evidence for the design and analysis of CC arches. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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21. A hybrid, game theory based, and distributed clustering protocol for wireless sensor networks.
- Author
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Yang, Liu, Lu, Yin-Zhi, Zhong, Yuan-Chang, Wu, Xue-Gang, and Xing, Shao-Jing
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GAME theory ,DISTRIBUTED algorithms ,ENERGY consumption ,WIRELESS sensor networks ,ITERATIVE methods (Mathematics) - Abstract
Clustering has been well known as an effective way to reduce energy dissipation and prolong network lifetime in wireless sensor networks. Recently, game theory has been used to model clustering problem. Each node is modeled as a player which can selfishly choose its own strategies to be a cluster head (CH) or not. And by playing a localized clustering game, it gets an equilibrium probability to be a CH that makes its payoff keep equilibrium. In this paper, based on game theory, we present a clustering protocol named Hybrid, Game Theory based and Distributed clustering. In our protocol, we specifically define the payoff for each node when choosing different strategies, where both node degree and distance to base station are considered. Under this definition, each node gets its equilibrium probability by playing the game. And it decides whether to be a CH based on this equilibrium probability that can achieve a good trade-off between minimizing energy dissipation and providing the required services effectively. Moreover, an iterative algorithm is proposed to select the final CHs from the potential CHs according to a hybrid of residual energy and the number of neighboring potential CHs. Our iterative algorithm can balance the energy consumption among nodes and avoid the case that more than one CH occurs in a close proximity. And we prove it terminates in finite iterations. Simulation results show that our protocol outperforms LEACH, CROSS and LGCA in terms of network lifetime. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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22. Mechanisms of human amniotic epithelial cell transplantation in treating stage III pressure ulcer in a rat model.
- Author
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AITING ZHOU, XILAN ZHENG, LIMEI YU, MINGTAO QUAN, XING SHAO, and ZHIXIA JIANG
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EPITHELIAL cells ,BEDSORES treatment ,AMNIOTIC liquid ,LABORATORY rats ,WOUND healing ,PROTEIN expression ,TRANSPLANTATION of organs, tissues, etc. - Abstract
The aim of the present study was to determine the function of human amniotic epithelial cell transplantation (hAECT) in promoting the healing of rats with stage III pressure ulcer (PU) and to initially investigate its possible mechanisms. A total of 96 Sprague Dawley rats were allocated at random into the model, hAECT, conventional treatment or control groups (n=24 per group). In each group, 6 rats were observed to determine the wound-healing rate. The mRNA and protein expression levels of vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF)-α in the wound tissue and serum were detected using reverse transcription-quantitative polymerase chain reaction analysis and enzyme-linked immunosorbent assay. The transplantation of hAECs significantly increased the healing rate of the stage III PUs and was accompanied by the significant upregulation of VEGF mRNA and protein expression levels and the significant downregulation of TNF-α mRNA and protein expression. Immunofluorescence staining showed that, on day 7 of transplantation, hAECs remained alive inside the skin tissues. Therefore, hAECT through subcutaneous injection appears to significantly improve the wound-healing rate of stage III PUs in rats, and this effect may be associated with the upregulation of the proangiogenic factor VEGF and the downregulation of the inflammatory cytokine TNF-α. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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23. Metabolite profiling of gypenoside LVI in rat after oral and intravenous administration.
- Author
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Chen, Dao-Jin, Hu, Hua-Gang, Xing, Shao-Fang, Liu, Hui-Min, and Piao, Xiang-Lan
- Abstract
Gypenoside LVI, one of the major bioactive triterpene saponins in Gynostemma pentaphyllum, has been regarded as a potential and promising lead drug for anti-tumor strategy. To better understand the pharmacological activities of the component, an investigation of its in vivo metabolism is important and necessary. In the present study, a liquid chromatography-ion trap time of flight tandem mass spectrometry has been utilized to discover and identify the metabolites of gypenoside LVI in rat urine after oral and intravenous administration. Negative electrospray ionisation mass spectrometry was used to discern gypenoside LVI and its possible metabolites in urine samples. As a result, after oral and intravenous administration, eight and six metabolites together with gypenoside LVI were detected and identified in rat urine, respectively. As metabolites of gypenoside LVI, they have never been reported before. Deglycosylation and dehydration were found to be the major metabolic processes of gypenoside LVI in rat. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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24. Robust stability regions of PID parameters for magnetic bearing based on D-partition technique.
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LUO Min-zhou, XING Shao-bang, YANG Yi-fei, LI Yue-hong, HAN Xiao-xin, and ZHU Huang-qiu
- Abstract
A method was introduced for finding all proportional integral derivation (PID) controllers that satisfy a robust performance constraint for a given transfer function of magnetic bearing with time-delay by using the frequency response. The H-infinity index of additive weight uncertainty on the basis of robustness stability condition of closed loop system was first got by small gain theorem. Then, Based on the boundary crossing theorem, the parameter boundaries with the expected decay ratio index of PID controller were derived. The system can be stabilized and robustly meet the performance requirements by choosing PID controllers parameter in the stability region. The proposed method avoids the complexity of stability regions of PID parameters, and the closed-loop system has strong robustness for magnetic bearing with time-delay system. The simulation and experiment results demonstrate the validity of this method. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
25. Efficacy and safety of transdermal fentanyl for the treatment of oral mucositis pain caused by chemoradiotherapy in patients with esophageal squamous cell carcinoma.
- Author
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Xing, Shao-Zhi and Zhang, Ying
- Subjects
MUCOSITIS ,SQUAMOUS cell carcinoma ,CANCER chemotherapy ,RADIOTHERAPY ,PAIN management - Abstract
Purpose: Oral mucositis is one of the most painful side effects found in esophageal squamous cell carcinoma (ESCC) patients treated with chemoradiotherapy. The transdermal route of administration is worthy of investigation for patients who suffer from dysphagia due to severe oral mucositis. In this phase 2 study, we investigated the efficacy and safety of transdermal fentanyl (TDF) for mucositis pain caused by chemoradiotherapy in ESCC patients. Methods: Forty-six ESCC patients who experienced moderate to severe oral mucosal pain during chemoradiotherapy received TDF for pain relief. The assessment of pain was made according to the Numeric Rating Scale (NRS). Efficacy and safety of TDF was collected and conducted in an open-label fashion. The analgesic effect, quality of life, and side effects were evaluated after the administration of transdermal fentanyl using the paired sample Wilcoxon signed rank test. Results: The mucositis-induced pain disappeared in 31 (67.4 %) patients during the treatment with transdermal fentanyl with the median time of onset at day 6.6 (range 3-14). The median Numeric Rating Scale (NRS) score was reduced from 6 (range 3-9) before treatment to 4.5 (range 2-9), 3 (range 2-8), 2.5 (range 1-8), 2 (range 0-6), and 0 (range 0-4) on days 3, 6, 9, 11, and 15, respectively, after treatment ( P < 0.001). The patients' quality of life also improved significantly ( P < 0.01). The side effects of treatment were mild and disappeared within several days. Conclusion: Transdermal fentanyl is an effective, convenient, and well-tolerated treatment for mucositis pain caused by chemoradiotherapy, which can improve ESCC patients' quality of life. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
26. Network coding based Energy Efficient Multicast Routing for wireless sensor network.
- Author
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Xing Shao, Cuixiang Wang, Huihui Xiang, and Jincheng Huang
- Published
- 2013
- Full Text
- View/download PDF
27. A study on frequency correlation characteristics over multipath channels.
- Author
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Jincheng Huang, Xing Shao, Huihui Xiang, and Matsufuji, Shinya
- Published
- 2013
- Full Text
- View/download PDF
28. Change of the cell cycle after flutamide treatment in prostate cancer cells and its molecular mechanism.
- Author
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Yong Wang, Chen Shao, Chang-Hong Shi, Lei Zhang, Hong-Kong Yue, Peng-Fei Wang, Bo Yang, Yun-Tao Zhang, Fan Liu, Wei-Jun Qin, He Wang, and Guo-Xing Shao
- Subjects
ANDROGENS ,SEX hormones ,MESSENGER RNA ,CELL cycle ,CELL culture ,DNA polymerases ,POLYMERASE chain reaction - Abstract
Aim: To explore the effect of androgen receptor (AR) on the expression of the cell cycle-related genes, such as CDKN1A and BTG1, in prostate cancer cell line LNCaP. Methods: After AR antagonist flutamide treatment and confirmation of its effect by phase contrast microscope and flow cytometry, the differential expression of the cell cycle-related genes was analyzed by a cDNA microarray. The flutamide treated cells were set as the experimental group and the LNCaP cells as the control. We labeled cDNA probes of the experimental group and control group with Cy5 and Cy3 dyes, respectively, through reverse transcription. Then we hybridized the cDNA probes with cDNA microarrays, which contained 8 126 unique human cDNA sequences and the chip was scanned to get the fluorescent values of Cy5 and Cy3 on each spot. After primary analysis, reverse transcription polymerase chain reaction (RT-PCR) tests were carried out to confirm the results of the chips. Results: After AR antagonist flutamide treatment, three hundred and twenty-six genes (3.93 %) expressed differentially, 97 down-regulated and 219 up-regulated. Among them, eight up-regulated genes might be cell cycle-related, namely CDC10, NRAS, BTG1, Wee1, CLK3, DKFZP564A122, CDKN1A and BTG2. The CDKN1A and BTG1 gene mRNA expression was confirmed to be higher in the experimental group by RT-PCR, while p53 mRNA expression had no significant changes. Conclusion: Flutamide treatment might up-regulate CDKN1A and BTG1 expression in prostate cancer cells. The protein expressions of CDKN1A and BTG1 play an important role in inhibiting the proliferation of cancer cells. CDKN1A has a great impact on the cell cycle of prostate cancer cells and may play a role in the cancer cells in a p53-independent pathway. The prostate cancer cells might affect the cell cycle-related genes by activating AR and thus break the cell cycle control. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
29. Phylogenetic Analysis of Eight Genes of H9N2 Subtype Influenza Virus: A Mainland China Strain Possessing Early Isolates Genes that have been Circulating.
- Author
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Jian-Hong Lu, Xiu-Fan Liu, Wei-Xing Shao, Yu-Liang Liu, Dong-Ping Wei, and Hong-Qi Liu
- Subjects
PHYLOGENY ,ORTHOMYXOVIRUSES ,INFLUENZA ,VIRUS diseases in poultry - Abstract
Abstract H9N2 subtype influenza virus has become worldwide and prevalent in China. Previous studies illustrated that at least three sublineages had been established in terrestrial poultry of Eurasian avian. In this presentation, eight full-length genes of an H9N2 strain, A/Chicken/Shanghai/F/98 (Ck/SH/F/98) were obtained. Sequence analysis and phylogenetic studies were conducted by comparing eight genes with those of all the available H9N2 strains from the GenBank. The results showed that four genes (HA, NA, M and NS genes) of Ck/SH/F/98 were incorporated into the sublineage represented by the early mainland China strain, Ck/BJ/1/94. However, the other four of RNP genes of Ck/SH/F/98 did not show close relationship with those of the three known sublineages viruses. Therefore, Ck/SH/F/98 was a natural reassortant between different sublineages. In addition, comparison showed that Ck/SH/F/98 could be a putative precursor of a later isolate from southern China, Dk/ST/1605/01, with at least six genes of both closely related, indicating genes of Ck/SH/F/98 and early isolates had ever been circulating. Further comparison in terms of molecular markers of species specificity of HA1 revealed that DK/ST/1605/01 also resembled Ck/SH/F/98 more than a common earlier duck strain. The results supported the idea of two-way transmission between terrestrial and aquatic birds that emphasized the importance to raise concerns on the natural evolution of all the eight genes of H9N2 avian influenza viruses. [ABSTRACT FROM AUTHOR]
- Published
- 2005
30. Syntheses of Benzimidazoles, Quinoxalines and 3,3-Dihydro-1 H-1,5-benzodiazepines Starting from o-Phenylenediamine.
- Author
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Yong, Cui, Xiu-Bo, Tang, Chang-Xing, Shao, Ji-Tai, Li, and Wen-Hua, Sun
- Published
- 2005
- Full Text
- View/download PDF
31. ChemInform Abstract: A New Dammarane-Type Saponin from Gynostemma pentaphyllum Induces Apoptosis in A549 Human Lung Carcinoma Cells.
- Author
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Xing, Shao‐Fang, Jang, Moonhee, Wang, Yu‐Rong, and Piao, Xiang‐Lan
- Published
- 2016
- Full Text
- View/download PDF
32. Cytotoxic activity of gypenosides and gynogenin from Gynostemma pentaphyllum against A549 cells (647.25).
- Author
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Piao, Xiang‐Lan, Chen, Dao‐Jin, Xing, Shao‐Fang, Kim, Sung Woo, and Piao, Xiangshu
- Published
- 2014
- Full Text
- View/download PDF
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