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In order to solve the problem of converting a large amount of original GNSS receiver observation data into RINEX format files independent of the receiver during satellite positioning measurement in railway line engineering plane control networks, this paper used the C # language for secondary development of the program. The GNSS receiver observation data of Trimble Company was taken as the research object, and the syntax rules of the batch processing file of the GNSS receiver original observation data which provided by the company decoding program Convert To RINEX were analyzed. Then, the batch processing file calling method of the program was used to develop the Trimble GNSS observation data batch processor. After engineering verification, this batch of processor software could conveniently and quickly convert the massive raw data of Trimble GNSS receivers into RINEX format files independent of the receivers, and can be applied to various large-scale satellite measurement projects such as railway line engineering plane control network satellite positioning measurement. [ABSTRACT FROM AUTHOR]

Sex differences in mammalian complex traits are prevalent and are intimately associated with androgens1–7. However, a molecular and cellular profile of sex differences and their modulation by androgens is still lacking. Here we constructed a high-dimensional single-cell transcriptomic atlas comprising over 2.3 million cells from 17 tissues in Mus musculus and explored the effects of sex and androgens on the molecular programs and cellular populations. In particular, we found that sex-biased immune gene expression and immune cell populations, such as group 2 innate lymphoid cells, were modulated by androgens. Integration with the UK Biobank dataset revealed potential cellular targets and risk gene enrichment in antigen presentation for sex-biased diseases. This study lays the groundwork for understanding the sex differences orchestrated by androgens and provides important evidence for targeting the androgen pathway as a broad therapeutic strategy for sex-biased diseases.The effects of sex and androgens on the molecular programs and cellular populations are explored using a single-cell transcriptomic atlas comprising over 2.3 million cells from different tissues in Mus musculus. [ABSTRACT FROM AUTHOR]

It is found during the tests that the damping characteristics of the magnetorheological (MR) damper vary with the excitation amplitude and frequency. However, the existing MR damper models are not able to accommodate the change of excitation amplitude and frequency, which will lead to significant modeling errors. To deal with this problem, this paper analyzes the experimental data and obtains the regularity of the damping characteristics varying with the excitation. Subsequently, an excitation-adaptive MR damper model is constructed based on the hyperbolic tangent function. The proposed model is not only able to adapt to the change of excitation amplitude and frequency but also able to inverse, which is essential for MR damper controller construction. The fitting results show that compared with the existing models, the three normalized errors of the proposed model are improved from 22.61%, 13.96%, and 19.42%–6.30%, 3.81%, and 6.97%, respectively, indicating that the model excitation adaptivity is significantly improved. Furthermore, this study also proposed a damper controller based on the new model, and the simulation results verify the effectiveness of the controller. The proposed model brings the acceleration signal into the model to improve the model adaptivity, which introduces a novel approach to enhance the adaptivity of MR damper models. [ABSTRACT FROM AUTHOR]

Purpose: For the drop-induced shock mitigation system with magnetorheological energy absorbers (MREAs), the time lag of MREA has a nonnegligible influence on the shock mitigation performance. This paper proposed a minimum transmitted load control method considering time lag (MTL-T) is proposed. Methods: First, the influence of a time lag on the shock mitigation performance is analyzed, and the relationship between the theoretical controllable force and the actual controllable force of the MREA with a time lag is given. Then, taking the controllable force of traditional MTL control method as the theoretical value and the "soft-landing" as the control target, the control algorithm of MTL-T control method is derived. Finally, the simulation is conducted for the shock mitigation system with variable parameters controlled by MTL-T, and the comparative performance of MTL-T control method and other methods is given. Results: The theoretical analysis shows that MTL-T control method can make the shock mitigation system with a time lag achieve the control target of "soft-landing". The advantages of MTL-T control method are shown by the comparative performance of the MTL-T control method and other methods. Conclusions: Once the parameters of the system and the impact intensity are obtained, the MTL-T can be determined. The adaptability of MTL-T control method is verified. Compared with other control method, the MTL-T has a small peak transmitted load while making full use of MREA. [ABSTRACT FROM AUTHOR]

In this paper, a new hysteresis model with resistor–capacitor (RC) operator for magnetorheological dampers (MRD) is proposed, which takes the excitation current, frequency, and amplitude as input variables. A hysteresis model based on RC operator is established, and the influence of each parameter in the model on the nonlinear behavior of MRD is given. The parameters are fitted based on the test result of an MRD with double rod. The fitting result of parameters shows that the amplitude and frequency of the excitation also affect the parameter values of the model. Base on this, the new hysteresis model is proposed. The parameter fitting results of the proposed model is good. The accuracy of the proposed model and the ability of the model to predict the nonlinear behavior of MRD has been validated by comparing with the experimental data and the RC operator-based model. The comparison results show that the proposed model has higher description accuracy and stronger prediction ability for nonlinear characteristics of MRD. [ABSTRACT FROM AUTHOR]

Metabolic fitness of T cells is essential for their vitality, which is largely dependent on the behavior of the mitochondria. The nature of mitochondrial behavior in tumor-infiltrating T cells remains poorly understood. In this study, we show that mitofusin-2 (MFN2) expression is positively correlated with the prognosis of multiple cancers. Genetic ablation of Mfn2 in CD8+ T cells dampens mitochondrial metabolism and function and promotes tumor progression. In tumor-infiltrating CD8+ T cells, MFN2 enhances mitochondria–endoplasmic reticulum (ER) contact by interacting with ER-embedded Ca2+-ATPase SERCA2, facilitating the mitochondrial Ca2+ influx required for efficient mitochondrial metabolism. MFN2 stimulates the ER Ca2+ retrieval activity of SERCA2, thereby preventing excessive mitochondrial Ca2+ accumulation and apoptosis. Elevating mitochondria-ER contact by increasing MFN2 in CD8+ T cells improves the efficacy of cancer immunotherapy. Thus, we reveal a tethering-and-buffering mechanism of organelle cross-talk that regulates the metabolic fitness of tumor-infiltrating CD8+ T cells and highlights the therapeutic potential of enhancing MFN2 expression to optimize T cell function. Editor's summary: Organelle cross-talk plays a fundamental role in cell biology, but how this cross-talk controls tumor-infiltrating CD8+ T cells (TILs) is not understood. Yang et al. investigate the role of MFN2 in CD8+ TILs, identifying that mitochondrial interaction with the endoplasmic reticulum (ER) is required for metabolic fitness and antitumor activity. Through interaction with SERCA2 on the ER, MFN2 was required for mitochondria-ER contact and Ca2+ homeostasis. MFN2 expression was associated with better survival in patients with cancer, and promoting MFN2 expression in CD8+ T cells improved response to immune checkpoint blockade in mice. Together, these findings identify a critical role for MFN2 in regulating the metabolism, function, and survival of CD8+ T cells and suggest that boosting MFN2 expression is an attractive avenue for improving immunotherapy. —Hannah Isles [ABSTRACT FROM AUTHOR]

Purpose: Human body is sensitive to low-frequency excitation, which can not be suppressed effectively by the suspension control strategy that only controls the damper. This paper focuses on improving the vehicles' vibration isolation performances in low-frequency excitation by adjusting the stiffness and damping of suspension simultaneously. Methods: By using the designed acceleration-driven-stiffness fuzzy control strategy (ADSFC) and Skyhook based fuzzy control strategy (SHFC), the internal pressure of air spring and the current of magnetorheological damper are controlled separately to realize the adjustment of stiffness and damping. Results: The simulation results show that, the performance of the proposed control strategies are better than the passive suspension in up-step excitation and random road excitation. In particular, by using ADS-SHFC, the peak values of the sprung mass acceleration, the suspension dynamic deflection and the tire dynamic deflection are reduced by 59.4%, 48.3% and 59.4% respectively comparing with the passive suspension. Conclusion: The proposed control strategies are different from the conventional control methodsthat only controls the damping, this study is one of the first attempts to enhance thelow-frequency vibration isolation by changing the stiffness using control strategies. The simulation results verify the effectiveness of the proposed control strategies to improve the vibration isolation performance under low-frequency excitation. [ABSTRACT FROM AUTHOR]

To obtain a comprehensive scenario of T cell profiles and synergistic immune responses, we performed single-cell RNA sequencing (scRNA-seq) on the peripheral T cells of 14 individuals with chronic human immunodeficiency virus 1 (HIV-1) infection, including nine treatment-naive (TP) and eight antiretroviral therapy (ART) participants (of whom three were paired with TP cases), and compared the results with four healthy donors (HD). Through analyzing the transcriptional profiles of CD4+ and CD8+ T cells, coupled with assembled T cell receptor sequences, we observed the significant loss of naive T cells, prolonged inflammation, and increased response to interferon-α in TP individuals, which could be partially restored by ART. Interestingly, we revealed that CD4+ and CD8+ Effector-GNLY clusters were expanded in TP cases, and persistently increased in ART individuals where they were typically correlated with poor immune restoration. This transcriptional dataset enables a deeper understanding of the pathogenesis of HIV-1 infection and is also a rich resource for developing novel immune targeted therapeutic strategies. [ABSTRACT FROM AUTHOR]

This paper proposed a minimum transmitted load (MTL) control method for drop-induced shock isolation mounts (SIM) with magnetorheological energy absorbers (MREAs). MTL control method consists of two parts of maximum damping force (MDF) control and one part of constant acceleration (CA) control, which can make the payload stop after fully utilize MREA stroke (soft landing) with minimum transmitted load. The control algorithm of MTL control method is derived in a single-degree-of-freedom (SDOF) system. The relationship between the controllable velocity range of MTL control method and parameters of shock isolation mounts is also derived. An optimal control method selection criterion between Bingham number (BN) control method and MTL control method is developed. The performance of MTL control method and selection criterion are shown by applying to the SIM system with variable drop velocities and system parameters. Results shows that MTL control method has the minimum transmitted load and the selection criterion is feasible. [ABSTRACT FROM AUTHOR]

This paper proposed an extended constant deceleration (ECD) control method that can be used in the shock mitigation system with magnetorheological energy absorbers (MREAs). The ECD control method has three sections: zero controllable force (ZCF) section, constant deceleration (CD) section, and maximum damping force (MDF) section. Under the control of ECD, the system can stop at the end of MREA stroke without exceeding the maximum allowable deceleration. The ECD control algorithm is derived in a single-degree-of-freedom (SDOF) system. The controllable velocity range and the required controllable damping force of ECD control method are also derived, which can provide feasible solutions for the design of shock isolation system with MREAs. The performance of ECD control method is shown by applying to the drop-induced shock mitigation system with different drop velocities, different maximum controllable damping force, and MREA stroke. The results shows that the ECD control method not only has a large controllable velocity range and small controllable damping force requirement, but also can minimize the load transmitted to the system. [ABSTRACT FROM AUTHOR]

In view of the dynamic phenomenon that coal and rock are susceptible to external impact disturbance in the mining process, combined with the rheological hypothesis mechanism of coal and gas outburst, the RLSS-II-type triaxial loading creep test system of gas-containing coal and rock developed by ourselves is used to carry out the conventional triaxial rheological test of gas-containing coal and rock and the rheological disturbance effect test of gas-containing coal and rock under impact disturbance. The strength limit neighborhood of gas-containing coal and rock is determined, and the gas-containing coal and rock entering the strength limit neighborhood are subjected to different impact disturbances. The experimental results show that, (1) under the confining pressure of 0 MPa, 2.5 MPa, and 5 MPa, the longitudinal deformations of gas-bearing coal and rock are 32 mm, 27 mm, and 22 mm, respectively, indicating that the deformation of coal and rock will be affected by confining pressure, and with the increase of confining pressure, the deformation will decrease. (2) In the test, the deformation of coal and rock in the late stage of uniform creep stage can be regarded as a strain threshold. Before this threshold, the strain of coal and rock is not obvious, and the deformation is only 1.1 mm. After exceeding a threshold, the deformation of coal and rock is 9 mm, and the deformation increases significantly. Then, it enters the accelerated creep stage quickly and finally damages. The vicinity of this threshold is called the strength limit neighborhood of coal and rock containing gas. (3) The gas-bearing coal and rock without entering the strength limit neighborhood and entering the strength limit neighborhood are changed by confining pressure and different impact disturbance, respectively. It is found that, whether in the strength limit neighborhood or outside the strength limit neighborhood, the confining pressure has an effect on the strain, but the influence is not large. Under different impact disturbance, the deformation of coal and rock within and outside the strength limit neighborhood is 8 mm and 0.4 mm, respectively, and the deformation changes obviously, indicating that the impact disturbance has a great influence on the deformation of coal and rock within the strength limit neighborhood, and the coal and rock with large impact disturbance are destroyed before the coal and rock with small impact disturbance, indicating that the greater the impact disturbance, the shorter the time required for destruction. [ABSTRACT FROM AUTHOR]

This research presents a minimal maximum deceleration (MMD) control method which can be used in the shock mitigation system with magnetorheological energy absorbers (MREAs). The proposed control method can make the payload stop at the end of the available MREA stroke with the lowest maximum deceleration, which does not exceed the deceleration threshold value and lead to the lowest occupant injury probability. The shock mitigation system controlled by MMD will experience constant deceleration control stage and maximum damping force control stage while making full use of the available MREA stroke. The comparative performance of the MMD control method with Bingham number (BN) control, constant deceleration (CD) control and minimum duration deceleration exposure (MDDE) control is shown. Then, the controllable drop velocity range and the required maximum MREA controllable damping force range of MMD control method is calculated. Subsequently, the optimal control method selection criterion among BN control method, CD control method and MMD control method is developed. Finally, the optimal selection criterion is applied to the drop induced shock mitigation system with varying payload velocity, payload mass (occupant type) and the maximum controllable damping force of MREA. [ABSTRACT FROM AUTHOR]

To obtain a comprehensive scenario of T cell profiles and synergistic immune responses, we performed single-cell RNA sequencing (scRNA-seq) on the peripheral T cells of 14 individuals with chronic human immunodeficiency virus 1 (HIV-1) infection, including nine treatment-naive (TP) and eight antiretroviral therapy (ART) participants (of whom three were paired with TP cases), and compared the results with four healthy donors (HD). Through analyzing the transcriptional profiles of CD4+ and CD8+ T cells, coupled with assembled T cell receptor sequences, we observed the significant loss of naive T cells, prolonged inflammation, and increased response to interferon-α in TP individuals, which could be partially restored by ART. Interestingly, we revealed that CD4+ and CD8+ Effector-GNLY clusters were expanded in TP cases, and persistently increased in ART individuals where they were typically correlated with poor immune restoration. This transcriptional dataset enables a deeper understanding of the pathogenesis of HIV-1 infection and is also a rich resource for developing novel immune targeted therapeutic strategies. [ABSTRACT FROM AUTHOR]

Lung cancer is the leading cause of cancer mortality, and early detection is key to improving survival. However, there are no reliable blood-based tests currently available for early-stage lung cancer diagnosis. Here, we performed single-cell RNA sequencing of different early-stage lung cancers and found that lipid metabolism was broadly dysregulated in different cell types, with glycerophospholipid metabolism as the most altered lipid metabolism–related pathway. Untargeted lipidomics was carried out in an exploratory cohort of 311 participants. Through support vector machine algorithm-based and mass spectrum–based feature selection, we identified nine lipids (lysophosphatidylcholines 16:0, 18:0, and 20:4; phosphatidylcholines 16:0–18:1, 16:0–18:2, 18:0–18:1, 18:0–18:2, and 16:0–22:6; and triglycerides 16:0–18:1–18:1) as the features most important for early-stage cancer detection. Using these nine features, we developed a liquid chromatography–mass spectrometry (MS)–based targeted assay using multiple reaction monitoring. This target assay achieved 100.00% specificity on an independent validation cohort. In a hospital-based lung cancer screening cohort of 1036 participants examined by low-dose computed tomography and a prospective clinical cohort containing 109 participants, the assay reached more than 90.00% sensitivity and 92.00% specificity. Accordingly, matrix-assisted laser desorption/ionization MS imaging confirmed that the selected lipids were differentially expressed in early-stage lung cancer tissues in situ. This method, designated as Lung Cancer Artificial Intelligence Detector, may be useful for early detection of lung cancer or large-scale screening of high-risk populations for cancer prevention. Lipidomics for early lung cancer detection: Improving early detection of lung cancer is key to improving outcomes. On the basis of their exploratory single-cell RNA sequencing suggesting dysregulated lipid metabolism in early lung cancer tissue, Wang et al. performed lung plasma lipidomic profiling and found that nine lipids were commonly dysregulated in patients with early-stage lung tumors. The authors validated the ability of these nine lipids to detect early-stage cancer across multiple independent cohorts largely composed of nonsmokers with stage I adenocarcinomas, as compared with healthy individuals or individuals with benign tumors. This study shows promise for the clinical detection of early-stage lung cancer in patients. [ABSTRACT FROM AUTHOR]

Background: Neurotropic virus infection can cause serious damage to the central nervous system (CNS) in both humans and animals. The complexity of the CNS poses unique challenges to investigate the infection of these viruses in the brain using traditional techniques. Methods: In this study, we explore the use of fluorescence micro‐optical sectioning tomography (fMOST) and single‐cell RNA sequencing (scRNA‐seq) to map the spatial and cellular distribution of a representative neurotropic virus, rabies virus (RABV), in the whole brain. Mice were inoculated with a lethal dose of a recombinant RABV encoding enhanced green fluorescent protein (EGFP) under different infection routes, and a three‐dimensional (3D) view of RABV distribution in the whole mouse brain was obtained using fMOST. Meanwhile, we pinpointed the cellular distribution of RABV by utilizing scRNA‐seq. Results: Our fMOST data provided the 3D view of a neurotropic virus in the whole mouse brain, which indicated that the spatial distribution of RABV in the brain was influenced by the infection route. Interestingly, we provided evidence that RABV could infect multiple nuclei related to fear independent of different infection routes. More surprisingly, our scRNA‐seq data revealed that besides neurons RABV could infect macrophages and the infiltrating macrophages played at least three different antiviral roles during RABV infection. Conclusion: This study draws a comprehensively spatial and cellular map of typical neurotropic virus infection in the mouse brain, providing a novel and insightful strategy to investigate the pathogenesis of RABV and other neurotropic viruses. [ABSTRACT FROM AUTHOR]

Background: Ribosome profiling has been widely used for studies of translation under a large variety of cellular and physiological contexts. Many of these studies have greatly benefitted from a series of data-mining tools designed for dissection of the translatome from different aspects. However, as the studies of translation advance quickly, the current toolbox still falls in short, and more specialized tools are in urgent need for deeper and more efficient mining of the important and new features of the translation landscapes. Results: Here, we present RiboMiner, a bioinformatics toolset for mining of multi-dimensional features of the translatome with ribosome profiling data. RiboMiner performs extensive quality assessment of the data and integrates a spectrum of tools for various metagene analyses of the ribosome footprints and for detailed analyses of multiple features related to translation regulation. Visualizations of all the results are available. Many of these analyses have not been provided by previous methods. RiboMiner is highly flexible, as the pipeline could be easily adapted and customized for different scopes and targets of the studies. Conclusions: Applications of RiboMiner on two published datasets did not only reproduced the main results reported before, but also generated novel insights into the translation regulation processes. Therefore, being complementary to the current tools, RiboMiner could be a valuable resource for dissections of the translation landscapes and the translation regulations by mining the ribosome profiling data more comprehensively and with higher resolution. RiboMiner is freely available at https://github.com/xryanglab/RiboMiner and https://pypi.org/project/RiboMiner. [ABSTRACT FROM AUTHOR]

1. The aim of this study was to develop a selective, rapid, accurate and sensitive ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for pharmacokinetic (PK) studies of phytoecdysones and triterpenoid saponins after oral administration of five monomers, crude, wine-processed and salt-processed Radix Achyranthis bidentatae (RAB). 2. A Thermo Hypersil GOLD C18 column (100 mm × 2.1 mm, 1.9 μm) coupled with a mobile phase of (A) acetonitrile and (B) water (both containing 0.3% acetic acid) was used for sample separation. The mass analysis was performed in a triple quadruple mass spectrometer using selected reaction monitoring (SRM) with negative scan mode. 3. The results showed that this method exhibited desirable sensitivity, precision, stability and repeatability. The extraction recoveries of the compounds ranged from 94.2 to 99.8% and the matrix effects ranged from 93.3 to 100.5%. Comparing the Cmax and AUC of five analytes in those groups showed this tendency: salt-processed RAB > wine-processed RAB > crude RAB > monomer group. The results confirmed the feasibility of TCM theory to enhance the efficacy of processed RAB. [ABSTRACT FROM AUTHOR]

Two new monoterpene glucosides: xanmonoter A (1) and xanmonoter B (2) were isolated from Xanthium strumarium. Their structures were elucidated on the basis of 1D and 2D NMR, MS and CD analysis. Compounds 1 and 2 were tested for their anti-inflammatory activity with IC50 values of 17.4, 22.1 μM, respectively. [ABSTRACT FROM AUTHOR]

A simple and sensitive analysis using ultra high performance liquid chromatography with a tandem mass spectrometric system operated in selected reaction monitoring mode was developed for the determination of 11 phenolic acids, atractyloside, and carboxyatractyloside in rat plasma. The two classes of analytes were then separated on a Waters ACQUITY™ UPLC HSS T3 column (50 mm × 2.1 mm, 1.8 µm) using gradient elution with a mobile phase of 0.2% formic acid in water containing 10 mM ammonium acetate and methanol. Detection was accomplished by selected reaction monitoring scanning via an electrospray source operating in negative ionization mode. The calibration curve was linear (R2 = 0.990) over a concentration range of 1.20–3500 ng/mL, while the validated lower limit of quantification was 1.20 ng/mL. The precision varied from 0.84 to 4.62%, and the accuracy varied within ±5%. The method proved robust with sample freezing and thawing and with short‐ and long‐term sample storage. The established method was used for simultaneous quantification and was successfully used for the first time for the pharmacokinetic evaluation of 13 compounds after the intragastric administration of raw and processed Fructus Xanthii in rats. The results indicated that processing affects the absorption and metabolism of Fructus Xanthii extract. Importantly, the results also indicated the importance of processing for the clinical application of traditional Chinese medicine. [ABSTRACT FROM AUTHOR]

The metabolic enzyme isocitrate dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate to α -ketoglutarate (α -KG). Its mutation often leads to aberrant gene expression in cancer. IDH1 was reported to bind thousands of RNA transcripts in a sequence-dependent manner; yet, the functional significance of this RNA-binding activity remains elusive. Here, we report that IDH1 promotes mRNA translation via direct associations with polysome mRNA and translation machinery. Comprehensive proteomic analysis in embryonic stem cells (ESCs) revealed striking enrichment of ribosomal proteins and translation regulators in IDH1-bound protein interactomes. We performed ribosomal profiling and analyzed mRNA transcripts that are associated with actively translating polysomes. Interestingly, knockout of IDH1 in ESCs led to significant downregulation of polysome-bound mRNA in IDH1 targets and subtle upregulation of ribosome densities at the start codon, indicating inefficient translation initiation upon loss of IDH1. Tethering IDH1 to a luciferase mRNA via the MS2-MBP system promotes luciferase translation, independently of the catalytic activity of IDH1. Intriguingly, IDH1 fails to enhance luciferase translation driven by an internal ribosome entry site. Together, these results reveal an unforeseen role of IDH1 in fine-tuning cap-dependent translation via the initiation step. [ABSTRACT FROM AUTHOR]

Abstract As a valuable herbal medicine, the fruits of Xanthium strumarium L. (Xanthii Fructus) have been widely used in raw and processed forms to achieve different therapeutic effects in practice. In this study, a comprehensive strategy was proposed for evaluating the active components in 30 batches of raw and processed Xanthii Fructus (RXF and PXF) samples, based on high-performance liquid chromatography coupled with photodiode array detection (HPLC-PDA). Twelve common peaks were detected and eight compounds of caffeoylquinic acids were simultaneously quantified in RXF and PXF. All the analytes were detected with satisfactory linearity (R2 > 0.9991) over wide concentration ranges. Simultaneously, the chemically latent information was revealed by hierarchical cluster analysis (HCA) and principal component analysis (PCA). The results suggest that there were significant differences between RXF and PXF from different regions in terms of the content of eight caffeoylquinic acids. Potential chemical markers for XF were found during processing by chemometrics [ABSTRACT FROM AUTHOR]

A simple and accurate method coupling high-performance liquid chromatography–evaporative light scattering detector was developed to simultaneously quantify the five triterpenoids in different parts of Aralia elata (Miq.) Seem (A. elata). for the first time. Samples were extracted using 70% methanol by ultrasonication. Separation of the analytes was performed on a Welchrom C18 column with acetonitrile and water as the mobile phase. All calibration curves had good linearity over relatively wide range of concentrations. This method showed good average recovery and precision, also high sensitivity with limits of detection and quantification. This method was successfully applied to quantify triterpenoid saponins in 14 samples collected from different areas of China. This study demonstrate that there is significant variation in the amounts of the five triterpenoids of interest in A. elata. Furthermore, 70% methanol extracts of roots had the strongest effect on ox-low-density lipoprotein-induced human umbilical vein endothelial cell apoptosis. [ABSTRACT FROM AUTHOR]

Background: Magnetic resonance imaging (MRI) can be used to guide breast cancer surgery with breast conservation for large tumors with a substantially reduced size after neoadjuvant chemotherapy (NAC).Purpose: To evaluate the value of dynamic contrast-enhanced MRI (DCE-MRI) for measuring residual tumor size and enhancement patterns following preoperative NAC.Material and Methods: Eighty-nine patients with breast cancer underwent breast DCE-MRI; 38 patients (39 lesions) were treated with NAC and examined for residual disease following therapy. Two patients were excluded because surgery had been performed >2 weeks after the final MR examination. Thus, we correlated the DCE-MRI results of 36 patients (37 lesions) with postoperative histopathological findings. Residual disease was confirmed by more enhancement compared to normal glandular tissue at the initial tumor site. Residual tumor size on DCE-MRI was compared with postoperative pathology findings. Tumor enhancement patterns on DCE-MRI were analyzed and correlated with pathological classification.Results: MRI revealed 34 cases of residual tumors, with two false positives and one false negative. Pathological and MR measurements were correlated (r = 0.793). The correlation of mass enhancement size (r = 0.87, n = 14) with pathology and DCE-MRI was higher than for non-mass-like enhancement (NME) (r = 0.735, n = 23). The distribution of pathologic classification was significantly different between different MRI enhancement patterns (P = 0.006). Mass enhancement had higher cellularity than NME.Conclusion: MRI is useful for evaluating residual carcinoma following NAC. Mass enhancement with higher cellularity after NAC can be evaluated more accurately, which is suitable for evaluating lumpectomy. However, other approaches are required for NME, which has lower cellularity. [ABSTRACT FROM AUTHOR]

Supercritical fluid chromatography is a safe and ecofriendly analytical technique that has not been fully applied to the analysis of traditional Chinese medicine. This is the first study on the separation of six quality markers—paeoniflorin, albiflorin, benzoyl paeoniflorin, oxypaeoniflorin, gallic acid and benzoic acid—from raw, wine‐baked and vinegar‐baked Paeoniae Alba Radix (PAR) by Supercritical fluid chromatography. Optimum separation was achieved on an HSS C18 SB column (100 × 3.0 mm, 1.8 μm particles) with a gradient elution of high‐purity carbon dioxide as mobile phase A and methanol–acetonitrile (70:30, v/v) with 0.10% phosphoric acid as mobile phase B. The flow rate was set at 0.7 mL/min for 15.0 min. The method was validated in terms of the overall intraday and interday precision, with relative standard deviations (RSDs) of 0.87–2.87 and 1.47–3.63%, respectively. The recoveries were 98.10–103.60% with an RSD of 1.00–3.40%. The stability of the RSD values was in the range 1.10–3.78%. The developed approach was successfully applied and provides a valuable reference for the quality assessment of PAR and processed PAR. The results also revealed that the standardization of processing technology is of great significance to the fluctuations in quality before and after the processing of traditional Chinese medicine. [ABSTRACT FROM AUTHOR]

In the study, a surface plasmon resonance-based (SPR-based) competitive assay was performed to analyze different compounds' inhibitory activity to TNF-α, an important pro-inflammatory cytokine in the pathogenesis of chronic inflammatory diseases. Moreover, the single mass spectrometry (MS) detection method was coupled with an ultra-high-performance liquid chromatography (UPLC) system for the routine quality control (QC) of a traditional Chinese medicine (TCM). The above quality control strategy was evaluated with Lonicera japonica Thunb. Analytes were firstly separated on a Waters ACQUITYTM UPLC HSS T3 column (2.1 × 50 mm; particle size = 1.8 μm) using a 0.1% formic acid gradient elution, then detected by negative ESI mass spectrometry. The limits of quantification (LOQ) for analytes reached 0.005–0.56 μg/mL. The LOD of the QDa detector was lower than that of the PDA detector, indicating its wider detection range. The QDa detector was also more suitable for the analysis of the complex matrix of TCM. The method showed excellent linearity, with regression coefficients higher than 0.9991. The average recoveries of the investigated analytes were in the range of 98.78–105.13%, with an RSD below 3.91%. The inter-day precision range (n = 3 days) was 2.51–4.54%. Compared to other detectors, this strategy could be widely applied in the quantitative analysis of TCM. In addition, the chemically latent data could be revealed using chemometric analysis. Importantly, this study provides an efficient screening method for small-molecule inhibitors targeting the TNF-α pathway. [ABSTRACT FROM AUTHOR]

The purpose of this study was to establish a rapid, reliable, and sensitive ultra-performance liquid chromatography with triple-quadrupole tandem mass spectrometry coupled with chemometric method to measure and evaluate the differences between thirteen compounds in raw and processed Tussilago farfara L. from different sources. This assay method was validated, and the results indicated that the calibration curves for the thirteen compounds had good linearity (R2 > 0.9990). The limits of detection and limits of quantification of the thirteen compounds ranged from 0.0012 to 0.0095 μg/mL and from 0.0038 to 0.0316 μg/mL, respectively. The relative standard deviations (RSD) of the intra- and inter-day precisions and stability ranged from 1.06 to 2.00%, 0.26 to 1.99%, and 0.75 to 1.97%, respectively. The sample recovery rates of the thirteen compounds with different concentrations were 94.47–104.06%. The chemometric results, including principal component analysis, hierarchical clustering analysis, three-dimensional analysis, and box plot analysis, indicated that there are significance differences in raw and processed Tussilago farfara L. The results of this study confirm that the proposed method is the first reported method that has been successfully applied for simultaneous determination and discovery of the difference between thirteen compounds of raw and processed Tussilago farfara L. Thus, this method could be a helpful tool for the detection and confirmation of the quality of traditional Chinese medicines and provide a basis for future pharmacological studies. [ABSTRACT FROM AUTHOR]

An accurate and reliable method using ultra-high performance liquid chromatography combined with triple quadrupole tandem mass spectrometry (UHPLC–MS/MS) was established for simultaneous quantification of five major bioactive analytes in raw, wine-processed, and salt-processed Radix Achyranthis bidentatae (RAB). The results showed that this method exhibited desirable sensitivity, precision, stability, and repeatability. The overall intra-day and inter-day variations (RSD) were in the range of 1.57–2.46 and 1.51–3.00%, respectively. The overall recoveries were 98.58–101.48% with a relative standard deviation (RSD) of 0.01–1.86%. In addition, the developed approach was applied to 21 batches of raw, wine-processed, and salt-processed samples of RAB. Hierarchical clustering analysis (HCA), principal component analysis (PCA), heat map, and boxplot analysis were performed to evaluate the quality of raw, wine-processed, and salt-processed RAB collected from different regions. The chemometrics combined with the quantitative analysis based on UHPLC–MS/MS results indicated that the content of five analytes increased significantly in processed RAB compared to raw RAB. [ABSTRACT FROM AUTHOR]