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The editorial in "Frontiers in Plant Science" discusses the significance of proanthocyanidins (PAs) and isoflavonoids in plants, highlighting their roles in plant defense, antioxidant properties, and nutritional quality of foods. Recent research has advanced our understanding of the biosynthesis of PAs and isoflavonoids, shedding light on enzyme structures, regulatory pathways, and novel mechanisms governing their formation. The editorial also includes studies on isoflavonoid biosynthesis in legumes, flavonoids in citrus fruits, anthocyanins in mung beans, and the pigmentation of naturally colored cotton varieties. Future research directions focus on exploring the spatial distribution and dynamics of PA and isoflavonoid biosynthesis, as well as understanding the interplay between environmental factors and metabolic processes to improve agricultural traits and nutritional quality. [Extracted from the article] more...

The aim of our study is to understand the patterns of variation in water use efficiency (WUE) in coniferous and broad-leaved mixed forest ecosystems across multiple scales and to identify its main controlling factors. We employ the eddy covariance method to gather data from 2017, 2018, and 2020, which we use to calculate the gross primary productivity and evapotranspiration of these forests in East China and to determine WUE at the ecosystem level. The mean daily variation in WUE ranges from 4.84 to 7.88 gC kg−1 H2O, with a mean value of 6.12 gC kg−1 H2O. We use ridge regression analysis to ascertain the independent effect of environmental factors on WUE variation. We find that WUE responds differently to environmental factors at different time scales. In mixed conifer ecosystems, temperature and relative humidity emerge as the most significant environmental factors influencing WUE variability. Especially at the seasonal scale, temperature and relative humidity can explain more than 51% of the WUE variation. Our results underscore the varied effects of environmental factors on WUE variation across different time scales and aid in predicting the response of WUE to climate change in coniferous and broad-leaved mixed forest ecosystems. [ABSTRACT FROM AUTHOR] more...

Summary: Plant tannins, including condensed tannins (CTs) and hydrolyzable tannins (HTs), are widely distributed in the plant kingdom. To date, tannase (TA) – is a type of tannin acyl‐hydrolase hydrolyzing HTs, CT monomer gallates and depsides – has been reported in microbes only. Whether plants express TA remains unknown.Herein, we report plant TA genes. A native Camellia sinensis TA (CsTA) is identified from leaves. Six TAs are cloned from tea, strawberry (Fragaria × ananassa, Fa) and four other crops. Biochemical analysis shows that the native CsTA and six recombinant TAs hydrolyze tannin compounds, depsides and phenolic glycosides.Transcriptional and metabolic analyses reveal that the expression of CsTA is oppositely associated with the accumulation of galloylated catechins. Moreover, the transient overexpression and RNA interference of FaTA are positively associated with the accumulation of ellagitannins in strawberry fruit. Phylogenetic analysis across different kingdoms shows that 29 plant TA homologs are clustered as a plant‐specific TA clade in class I carboxylesterases. Further analysis across the angiosperms reveals that these TA genes are dispersed in tannin‐rich plants, which share a single phylogenetic origin c. 120 million yr ago.Plant TA is discovered for the first time in the plant kingdom and is shown to be valuable to improve tannin compositions in plants. [ABSTRACT FROM AUTHOR] more...

Background: Boehmeria nivea L. Gaud (Ramie) produces one of the longest natural fibers in nature. The bark of ramie mainly comprises of the phloem tissue of stem and is the raw material for fiber. Therefore, identifying the molecular regulation of phloem development is important for understanding of bast fiber biosynthesis and improvement of fiber quality in ramie. Results: In this study, we collected top bud (TB), bark from internode elongating region (ER) and bark from internode fully elongated region (FER) from the ramie variety Zhongzhu No. 1. Histological study indicated that these samples contain phloem tissues at different developmental and maturation stages, with a higher degree of maturation of phloem tissue in FER. RNA sequencing (RNA-seq) was performed and de novo transcriptome was assembled. Unigenes and differentially expressed genes (DEGs) in these three samples were identified. The analysis of DEGs by using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed clear differences in gene expression between ER and FER. Some unigenes involved in secondary cell wall biosynthesis were up-regulated in both ER and FER, while unigenes for some cell wall components or cell wall modifications showed differential expression between ER and FER. In addition, the ethylene respond factors (ERFs) in the ethylene signaling pathway were up-regulated in FER, and ent-kaurenoic acid oxidase (KAO) and GA 20-oxidase (GA20ox) for gibberellins biosynthesis were up-regulated while GA 2-oxidase (GA2ox) for gibberellin inactivation was down-regulated in FER. Conclusions: Both morphological study and gene expression analysis supported a burst of phloem and vascular developmental processes during the fiber maturation in the ramie stem, and ethylene and gibberellin are likely to be involved in this process. Our findings provide novel insights into the phloem development and fiber maturation in ramie, which could be useful for fiber improvement in ramie and other fiber crops. [ABSTRACT FROM AUTHOR] more...

Key message: TFL1homologCorcanTFL1suppresses the initiation of inflorescence development and regulates the inflorescence morphology inCornus canadensis.Abstract: In flowering plants, there is a wide range of variation of inflorescence morphology. Despite the ecological and evolutionary importance, efforts devoted to the evolutionary study of the genetic basis of inflorescence morphology are far fewer compared to those on flower development. Our previous study on gene expression patterns suggested a CorTFL1-CorAP1 based model for the evolution of determinate umbels, heads, and mini dichasia from elongated inflorescences in Cornus. Here, we tested the function of CorcanTFL1 in regulating inflorescence development in Cornus canadensis through Agrobacterium-mediated transformation. We showed that transgenic plants overexpressing CorcanTFL1 displayed delayed or suppressed inflorescence initiation and development and extended periods of vegetative growth. Transgenic plants within which CorcanTFL1 had been down-regulated displayed earlier emergence of inflorescence and a reduction of bract and inflorescence sizes, conversions of leaves to bracts and axillary leaf buds to small inflorescences at the uppermost node bearing the inflorescence, or phyllotaxy changes of inflorescence branches and leaves from decussate opposite to spirally alternate. These observations support an important role of CorcanTFL1 in determining flowering time and the morphological destinies of leaves and buds at the node bearing the inflorescence. The evidence is in agreement with the predicted function of CorTFL1 from the gene expression model, supporting a key role of CorTFL1 in the evolutionary divergence of inflorescence forms in Cornus. [ABSTRACT FROM AUTHOR] more...

Carbon nanotube (CNT) has been received much interest due to its unique structure, mechanical and electronic properties. Although activated CNT can enhance the specific capacitance, the effect is limited. Polyaniline (PANI) has good mechanical performance, environmental stability and reversible control of conductivity both by charge-transfer doping and protonation. So the CNT/PANI composite is an effective method to improve the performance of sample. It is well know that the composite morphology is closely with its electrochemical properties. The fibrous composite materials with the large surface area have been received much interest for their potential electrochemical performance. In this paper, the CNT/PANI composite was prepared by the FeO as template. Firstly, the acyl chloride was grafted on the surface of the CNT by series of organic chemistry reactions and the CNT-COCl was got. Then, the CNT-COCl absorbed on the surface of the FeO. After that, the CNT-COCl was reaction with aniline and got the CNT amide. Then, the aniline grafted on the CNT was polymerized to PANI by in-situ chemical polymerization. Finally, the cotton-shaped CNT/PANI composite was obtained. The obtained material was characterized by the Fourier transform infrared spectroscopy, scanning electron microscope and transmission electron microscope. And the cyclic voltammograms, galvanostatic charging/discharging and cycle life testing were used to tested electrochemical performances of the obtained composite. The results show that the obtained CNT/PANI composite had high specific capacitance of 144 F/g (in organic electrolyte), which is much more than those of the pure PANI (about 71 F/g). So it may be found that the composite material has good application prospects in super capacitor field. [ABSTRACT FROM AUTHOR] more...

Despite increasing interest in the molecular mechanisms of floral diversity, few studies have investigated the developmental and genetic bases of petaloid bracts. This study examined morphological patterns of bract initiation and expression patterns of B-class MADS-box genes in bracts of several Cornus species. We suggest that petaloid bracts in this genus may not share a single evolutionary origin., Developmental pathways of bracts and spatiotemporal expression of B-class genes in bracts and flowers were examined for four closely related dogwood species., Divergent morphological progressions and gene expression patterns were found in the two sister lineages with petaloid bracts, represented by Cornus florida and Cornus canadensis. Phylogeny-based analysis identified developmental and gene expression changes that are correlated with the evolution of petaloid bracts in C. florida and C. canadensis., Our data support the existence of independent evolutionary origins of petaloid bracts in C. canadensis and C. florida. Additionally, we suggest that functional transference within B-class gene families may have contributed to the origin of bract petaloidy in C. florida. However, the underlying mechanisms of petaloid bract development likely differ between C. florida and C. canadensis. In the future this hypothesis can be tested by functional analyses of Cornus B-class genes. [ABSTRACT FROM AUTHOR] more...