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Improving vegetable yield and optimizing its quality through nutrient management have long been central to plant nutrition and horticultural science. Copper (Cu) is recognized as an essential trace element that promotes plant growth and development. However, the mechanisms by which Cu influences nitrogen (N) metabolism remain largely unknown, with limited studies exploring the interaction between Cu and varying nitrate-to-ammonium (nitrate/ammonium) ratios. In this study, Chinese cabbage was exposed to two Cu concentrations (0 and 0.02 mg L-1) in combination with three nitrate/ammonium ratios (10/90, 50/50, and 90/10) under hydroponic conditions. The results showed that Cu application increased plant biomass, nitrate reductase (NR) and glutamine synthetase (GS) enzyme activities, the expression of NR (NIA) and GS2 (Gln2) genes, and N content in both shoots and roots. Additionally, Cu treatment decreased nitrate and free amino acid contents, as well as the expression of nitrate transporters NRT1.1 and NRT2.1 in roots while increasing these four parameters in shoots. Additionally, these effects were significantly modulated by the nitrate/ammonium ratios. In conclusion, Cu may facilitate nitrate transportation, enhance nitrate reduction, promote ammonium assimilation, and influence the transformation of organic N compounds, highlighting its potential role in improving N metabolism in Chinese cabbage. [ABSTRACT FROM AUTHOR]

Improving vegetable yield and optimizing its quality through nutrient management have long been central to plant nutrition and horticultural science. Copper (Cu) is recognized as an essential trace element that promotes plant growth and development. However, the mechanisms by which Cu influences nitrogen (N) metabolism remain largely unknown, with limited studies exploring the interaction between Cu and varying nitrate-to-ammonium (nitrate/ammonium) ratios. In this study, Chinese cabbage was exposed to two Cu concentrations (0 and 0.02 mg L-1) in combination with three nitrate/ammonium ratios (10/90, 50/50, and 90/10) under hydroponic conditions. The results showed that Cu application increased plant biomass, nitrate reductase (NR) and glutamine synthetase (GS) enzyme activities, the expression of NR (NIA) and GS2 (Gln2) genes, and N content in both shoots and roots. Additionally, Cu treatment decreased nitrate and free amino acid contents, as well as the expression of nitrate transporters NRT1.1 and NRT2.1 in roots while increasing these four parameters in shoots. Additionally, these effects were significantly modulated by the nitrate/ammonium ratios. In conclusion, Cu may facilitate nitrate transportation, enhance nitrate reduction, promote ammonium assimilation, and influence the transformation of organic N compounds, highlighting its potential role in improving N metabolism in Chinese cabbage. [ABSTRACT FROM AUTHOR]

Biochar-mediated microbial degradation is receiving increased attention for remediating organic pollutants. However, the mechanism of biochar-facilitated bioremediation is poorly understood. This study demonstrated that wheat straw biochar could significantly enhance roxarsone biodegradation by Shewanella oneidensis MR-1. The average rate constants were calculated to be 0.0410, 0.0477, 0.0575, and 0.0441 h−1 for treatments with 0.05, 0.1, 0.5, and 1 g/L biochar, respectively, which were all higher than that in treatment with only MR-1 (0.0363 h−1). The impact of biochar dosage on roxarsone degradation followed as 0.5 g/L > 0.1 g/L > 1.0 g/L > 0.05 g/L biochar. The high specific surface area of biochar provided more opportunity for contact with MR-1, which was certified by the scanning electron microscope images. The extracellular polymeric substances were affected by the addition of biochar. The concentrations of protein and polysaccharide were lower with various concentrations of biochar to the system with only MR-1. The cell proliferation was promoted by the addition of biochar and the optical density value of MR-1 followed 0.5 g/L biochar group > 0.1 g/L biochar group > 1 g/L biochar group > 0.05 g/L biochar group > only MR-1. These results improved our understanding of biochar-mediated biodegradation and showed that an optimized dosage of wheat straw-derived biochar could be a microbial growth activator, accelerating the roxarsone conversion rate. [ABSTRACT FROM AUTHOR]

Background: This study explores the intricate link between vigorous physical activity and academic achievement in adolescents. By analyzing data from the Health Behaviour in School-aged Children (HBSC) survey, it seeks to understand how engaging in high-intensity physical activities influences academic achievement. Methods: A comprehensive cross-sectional analysis was conducted on a vast dataset comprising 214,808 adolescents aged 11, 13, and 15 years. The frequency of vigorous physical activity was determined through self-reports, while academic achievement was assessed based on students' selfperceptions, reflecting their understanding of teachers' evaluations of their academic performance. Results: Adolescents who reported engaging in vigorous physical activity daily were more likely to report higher academic achievement. This positive correlation was consistent across various demographic groups, including different age cohorts and both genders, indicating a universal benefit of regular vigorous physical activity on perceived academic success. Conclusion: The findings of this study underscore the significant role that regular vigorous physical activity plays in the academic lives of adolescents. The correlation between daily vigorous physical activity and enhanced selfreported academic achievement suggests that encouraging high-intensity physical activities in schools could be a key strategy in boosting educational outcomes. [ABSTRACT FROM AUTHOR]

Introduction: The Wuzhishan ant (MY) chicken exhibits significant differences from other chicken breeds. However, the molecular genetic relationship between the MY breed and other chicken breeds has not been assessed. Methods: Whole-genome resequencing was used to compare genetic diversity, nucleotide diversity, the fixation index, the linkage disequilibrium coefficient, and phylogenetic tree relationships between the MY breed and the Wenchang (WC), Danzhou (DZ), Bawangling (BW), and Longsheng Feng (LF) breeds. Results: A total of 21,586,378 singlenucleotide polymorphisms and 4,253,341 insertions/deletions were screened out among the five breeds. The MY breed had the second highest genomic genetic diversity and nucleotide diversity and the lowest LD coefficient among the five breeds. Moreover, the phylogenetic tree analysis showed that individual birds of each breed clustered together with those of their respective breeds. Discussion: Our data indicated that the MY breed is distinct from the other breeds and can be considered a new genetic resource. [ABSTRACT FROM AUTHOR]

In the process of bottom blowing bath smelting, continuous bubbles are formed and rise in the melt after the bottom blowing gas is ejected. In order to reveal the motion behavior and wake characteristics of continuous bubbles formed during the injection process, the rising process of coaxial double bubbles is studied through numerical simulation, the velocity and deformation characteristics of coaxial double bubbles in the rising process are obtained. Based on the vortex identification reconstruction of the flow field, the characteristics of formation, evolution, and configuration of bubble wake are obtained. The results show that in terms of motion characteristics, the aspect ratio of the upper bubble is always less than 1, and lower bubble has a large span of change: the minimum is 0.85 and the maximum is 1.2. As the two bubbles approach, the aspect ratio of the upper bubble is always less than 1, while the maximum aspect ratio of the lower bubble can reach 1.2. In terms of wake characteristics, the vorticity on the upper bubble surface is larger. When the wake rotation centers of the upper and lower bubbles merge with each other, the instantaneous acceleration of the lower bubble reaches the maximum. [ABSTRACT FROM AUTHOR]

Avian coccidiosis, a parasitic disease prevalent in poultry, is caused by Eimeria species and leads to significant economic losses. The use of attenuated live oocyst vaccines has been adopted as an alternative to the use of anticoccidial drugs. However, the accurate detection and differentiation of vaccine strains from virulent ones remain challenging. Therefore, this study presents a novel TaqMan polymerase chain reaction (PCR) detection method that offers enhanced sensitivity, specificity, and reproducibility compared with traditional PCR techniques. Through whole-genome resequencing and bioinformatics analysis, we identified a molecular marker gene, Em_marker6, with a unique 21-base pair deletion specific to the Eimeria maxima attenuated vaccine strain. Optimized primers and probes targeting this marker enabled rapid quantification cycle value achievement and high fluorescence intensity. The standard curve's slope of -3.540 and correlation coefficient of 0.9971 confirmed precise quantification capabilities. The TaqMan PCR method detected as few as 30 plasmid DNA copies and 50 oocysts per reaction, outperforming traditional PCR techniques by an order of magnitude. No cross-reactivity was observed with other E. maxima wide-type strains or common intestinal pathogens, ensuring the exclusive detection of the E. maxima EMPY vaccine strain. Weekly testing over 3 weeks demonstrated minimal variability, indicating robust consistency in the method's application. Testing on 61 clinical samples revealed a 57.38% positivity rate for E. maxima species and 13.11% for the vaccine strain. The Em_marker6 gene exhibited genetic stability across multiple generations, confirming the detection method's robust stability for the attenuated E. maxima vaccine strain. This study significantly advances the field of avian coccidiosis research and control by providing a valuable tool for monitoring vaccine purity and preventing inadvertent infections in vaccinated flocks, aligning with global efforts to curb antibiotic use in animal feed. [ABSTRACT FROM AUTHOR]

Nanoparticles have the capability to effectively improve the thermal conductivity of base fluids, thus improving the heat transfer coefficient of heat transfer systems. In this study, a non-equilibrium molecular dynamics method based on the Fourier law is employed to study the thermal conductivity of TiO2 (r-TiO2)-water nanofluids with temperatures ranging between 303 K and 333 K and volume fractions in the range of 1%-2%. The ordered layer structure as a shell is analyzed and its influence is surveyed by calculating the number density and radial distribution function. The results revealed that a clear, solid-like nanolayer of about 0.5 nm can be observed around the nanoparticle. In this regard, the thickness of the nanolayer is less affected by variations in volume fraction and temperature. The g(r) values and the number density decreased with the increase in temperature. Additionally, the g(r) values and the number density at the level of the nanolayer were much higher compared to those at other parts. This indicates the existence of more water molecules in the nanolayer, thereby reducing contact thermal resistance and improving thermal conductivity. Macroscopically, the thermal conductivity increases with the increase in volume fraction. It was found that the increase in the volume fraction from 1%-2% at 303 K resulted in an increase in the effective thermal conductivity from 1.027 and 1.042, respectively. In other words, the thermal conductivity of the nanofluid was 2.7% and 4.2% higher than that of the base liquid under the same conditions. [ABSTRACT FROM AUTHOR]

Significance: Endoscopic screening for esophageal cancer (EC) may enable early cancer diagnosis and treatment. While optical microendoscopic technology has shown promise in improving specificity, the limited field of view (<1 mm) significantly reduces the ability to survey large areas efficiently in EC screening. Aim: To improve the efficiency of endoscopic screening, we propose a novel concept of end-expandable endoscopic optical fiber probe for larger field of visualization and for the first time evaluate a deep-learning-based image super-resolution (DL-SR) method to overcome the issue of limited sampling capability. Approach: To demonstrate feasibility of the end-expandable optical fiber probe, DL-SR was applied on simulated low-resolution microendoscopic images to generate super-resolved (SR) ones. Varying the degradation model of image data acquisition, we identified the optimal parameters for optical fiber probe prototyping. The proposed screening method was validated with a human pathology reading study. Results: For various degradation parameters considered, the DL-SR method demonstrated different levels of improvement of traditional measures of image quality. The endoscopists’ interpretations of the SR images were comparable to those performed on the high-resolution ones. Conclusions: This work suggests avenues for development of DL-SR-enabled sparse image reconstruction to improve high-yield EC screening and similar clinical applications. [ABSTRACT FROM AUTHOR]

Introduction: The host immune response determines the differential outcome of acute or chronic viral infections. The comprehensive comparison of lymphoid tissue immune cells at the single-cell level between acute and chronic viral infections is largely insufficient. Methods: To explore the landscape of immune responses to acute and chronic viral infections, single-cell RNA sequencing(scRNA-seq), scTCR-seq and scBCRseq were utilized to evaluate the longitudinal dynamics and heterogeneity of lymph node CD45+ immune cells in mouse models of acute (LCMV Armstrong) and chronic (LCMV clone 13) viral infections. Results: In contrast with acute viral infection, chronic viral infection distinctly induced more robust NK cells and plasma cells at the early stage (Day 4 postinfection) and acute stage (Day 8 post-infection), respectively. Moreover, chronic viral infection exerted decreased but aberrantly activated plasmacytoid dendritic cells (pDCs) at the acute phase. Simultaneously, there were significantly increased IgA+ plasma cells (MALT B cells) but differential usage of B-cell receptors in chronic infection. In terms of T-cell responses, Gzma-high effector-like CD8+ T cells were significantly induced at the early stage in chronic infection, which showed temporally reversed gene expression throughout viral infection and the differential usage of the most dominant TCR clonotype. Chronic infection also induced more robust CD4+ T cell responses, including follicular helper T cells (Tfh) and regulatory T cells (Treg). In addition, chronic infection compromised the TCR diversity in both CD8+ and CD4+ T cells. Discussion: In conclusion, gene expression and TCR/BCR immune repertoire profiling at the single-cell level in this study provide new insights into the dynamic and differential immune responses to acute and chronic viral infections. [ABSTRACT FROM AUTHOR]

Introduction: Attempts to artificially activate unfertilized oocytes at 24 h post intracytoplasmic sperm injection (ICSI) have generally resulted in poor outcomes. This study aims to explore a new strategy for early judgement and rescue activation of unfertilized oocytes at 5 h post ICSI to avoid unexpected fertilization failure (UFF) or unexpected low fertilization (ULF) in ICSI cycles. Methods: Firstly, time-lapse data from 278 ICSI cycles were retrospectively analyzed to establish an indicator for fertilization failure prediction. Secondly, 14 UFF and 20 ULF cycles were enrolled for an observational study, early rescue oocyte activation (EROA) was performed on oocytes without post-ICSI Pb2 extrusion to investigate fertilization efficiency, embryo development and clinical outcomes. Results: The average time to Pb2 extrusion post-ICSI was 3.03±1.21 h, 95.54% of oocytes had extruded Pb2 before 5 h, and the sensitivity and specificity for monitoring Pb2 extrusion at 5 h by time-lapse imaging to predict fertilization were 99.59% and 99.78%, respectively. Early rescue activation of oocytes with no Pb2 extrusion resulted in acceptable fertilization and embryo developmental outcomes, in terms of the fertilization rate (75.00, 72.99%), 2PN fertilization rate (61.36, 56.93%), good-quality embryo rate (42.59, 50.00%), blastocyst formation rate (48.28, 46.03%), good-quality blastocyst rate (34.48, 33.33%), and oocyte utilization rate (36.36, 27.74%), for both UFF and ULF cycles. The clinical pregnancy, embryo implantation, and early miscarriage rates in the rescue oocyte activation group did not significantly differ from those in the Pb2 extrusion group. Fourteen unexpected fertilization failures and 20 low fertilization ICSI cycles were rescued and resulted in clinical pregnancy rates of 40.00% (4/10) and 57.14% (8/14), respectively. Conclusions: This study demonstrates that monitoring Pb2 extrusion by time-lapse imaging can accurately predict fertilization outcomes, suggesting that early rescue oocyte activation at 5 h post ICSI is an effective strategy for avoiding unexpected fertilization failure and low fertilization in ICSI cycles. [ABSTRACT FROM AUTHOR]

Background: Cardiovascular calcification includes cardiac valve calcification (CVC) and vascular calcification. We aimed to analyze risk factors for CVC, and construct a predictive model in maintenance peritoneal dialysis (MPD) patients. Methods: We retrospectively analyzed MPD patients who began peritoneal dialysis between January 2014 and September 2021. Patients were randomly assigned to the derivation cohort and validation cohort in a 7:3 ratio. The patients in the derivation cohort were divided into the CVC group and non-CVC group. Logistic regression was used to analyze risk factors, then the rms package in R language was used to construct a nomogram model to predict CVC. Results: 1,035 MPD patients were included, with the age of 50.0 ± 14.2 years and 632 males (61.1%). Their median follow-up time was 25 (12, 46) months. The new-onset CVC occurred in 128 patients (12.4%). In the derivation cohort, multivariate logistic regression indicated old age, female, high systolic blood pressure (SBP), high calcium-phosphorus product (Ca × P), high Charlson comorbidity index (CCI) and long dialysis time were independent risk factors for CVC (p < 0.05). We constructed a nomogram model for predicting CVC in the derivation cohort, with a C index of 0.845 (95% CI 0.803-0.886). This model was validated with a C index of 0.845 (95%CI 0.781-0.909) in the validation cohort. Conclusion: We constructed a nomogram model for CVC in MPD patients, using independent risk factors including age, sex, SBP, Ca × P, CCI and dialysis time. This model achieved high efficiency in CVC prediction. [ABSTRACT FROM AUTHOR]

Sustainable development is the central theme of modern global development. With the arrival of the urban era, the vulnerability and instability of rural areas have significantly increased, and rural sustainable development faces serious challenges. To address these issues, the study took the Zaozhuang Innovation Demonstration Zone in China under the National Sustainable Development Agenda as a case, combined with 2016–2020 economic and social and land use data, and applied Granger causality test method to explore the theoretical and practical pathways of “innovation-driven rural sustainable development”. The results showed that rural sustainable development and economic sustainability displayed a trend of synergistic change, with “explosive” growth from 2018 to 2020. The social sustainability steadily increased from 2016 to 2020. Ecological and spatial sustainability continuously declined during the study period. Moreover, the rural innovation capacity of the Zaozhuang Innovation Demonstration Zone displayed rapid growth during 2016–2020. Although the rural innovation capacity of the Zaozhuang Innovation Demonstration Zone has rapidly improved, it has a weak driving effect on rural sustainable development and economic sustainability. There are two primary challenges that must be overcome to ensure the rural sustainable development of the Zaozhuang Innovation Demonstration Zone. The first challenge is the imbalance among the multi-dimensional relationships in the process of rural sustainable development, and the second challenge is the weakening of rural innovation capacity to drive rural sustainable development. To overcome these challenges, this study proposed a systematic pathway for rural sustainable development in the Zaozhuang Innovation Demonstration Zone from multi-dimensions, such as policy actions, technologies, projects, and institutional guarantees, and formed a universal and representative “Zaozhuang model”. This study expands the theoretical foundation of rural sustainable development and provides theoretical and practical support for innovation-driven rural sustainable development. [ABSTRACT FROM AUTHOR]

This article investigates the effect of different magnetic fields on modulating the alignment of graphene nanoplatelets (GNPs) and their impact on the thermal conductivity of epoxy nanocomposites. Various nanocomposite specimens were fabricated with different GNP contents by using magnetically fieldaligned GNPs in the fabrication process to enhance the thermal conductivity. The results showed that the in-plane thermal conductivity of the Bmf (Bidirectional magnetic field) sample with 5 wt% GNPs added reached 0.75 W m-1 K-1, which is 276.6% higher than that of pure epoxy resin and the anisotropy coefficient (Kk/K?) reached 3.3. In addition, temperature rise tests were performed to simulate the thermal conductivity of nanocomposites used as an electronic packaging material, and the results indicated that the composites achieved a significant increase in in-plane thermal conductivity with a lower amount of filler. This provides valuable insights into the preparation of thermally conductive composites and demonstrates their potential as a thermal management packaging material for next-generation electronic devices. [ABSTRACT FROM AUTHOR]

Introduction: Hereditary antithrombin-III deficiency can significantly increase the risk for thrombosis, which is common in limb deep vein and pulmonary cases. However, thrombotic microangiopathy (TMA) caused by hereditary antithrombin deficiency is rare. Case Presentation: We reported the case of a 32-year-old Chinese female patient with TMA with renal injury caused by decreased antithrombin-III activity due to a new mutation (chr1-173884049 c.50A>G) in SERPINC1, which encodes antithrombin-III. In this case, the patient had no history of relevant drug use, diabetes, or monoclonal plasma cells in the bone marrow puncture. Consequently, TMA of the kidney was considered secondary to hereditary antithrombin-III deficiency. Gene detection was the only clue that led us to suspect that TMA was caused by hereditary antithrombin deficiency. Conclusion: Our findings indicated that for patients with repeated findings of antithrombin-III activity less than 50%, the possibility of antithrombin-III deficiency and complete gene detection must be considered immediately after excluding the use of anticoagulants and lack of availability to facilitate early detection, diagnosis, and intervention. [ABSTRACT FROM AUTHOR]

Purpose: The purpose of this study was to provide a critical systematic review of the role of magnetic resonance imaging (MRI) as a noninvasive method to assess periprosthetic joint infections (PJIs). Methods: The electronic databases PubMed and EMBASE were searched, since their inception up to March 27, 2022. The included studies evaluated the reproducibility and accuracy of MRI features to diagnose PJIs. The article quality assessment was conducted by the COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN) and Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2). Results: Among 1909 studies identified in the initial search, 8 studies were eligible for final systematic review. The included studies evaluated the reproducibility and accuracy of MRI features to diagnose PJIs. Seven of 8 studies showed good to excellent reliability, but only one article among them in which accuracy was evaluated had a low risk of bias. The intraclass correlation coefficient (ICC) and Cohen coefficient (κ) varied between 0.44 and 1.00. The accuracy varied between 63.9% and 94.4%. Potential MRI features, such as lamellated hyperintense synovitis, edema, fluid collection, or lymphadenopathy, might be valuable for diagnosing PJIs. Conclusion: The quality of the evidence regarding the role of MRI for PJIs diagnosis was low. There is preliminary evidence that MRI has a noteworthy value of distinguishing suspected periprosthetic joint infection in patients with total knee arthroplasty or total hip arthroplasty, but the definition of specific MRI features related to PJIs diagnosis lacks consensus and standardization. Large-scale studies with robust quality were required to help make better clinical decisions in the future. [ABSTRACT FROM AUTHOR]

Background: Does short-interval second ejaculation improve sperm quality, embryo development and clinical outcomes for oligoasthenozoospermia males received intracytoplasmic sperm injection (ICSI) treatment? Methods: All enrolled male patients underwent short-interval secondary ejaculation on the day of oocyte retrieval, and 786 sibling MII oocytes from 67 cycles were equally divided into two groups based on whether the injected spermatozoons originated from the first or second ejaculation. Semen parameters, embryo development efficiency, morphokinetic parameters and clinical outcomes were compared between the two groups to assess the efficiency and clinical value of short-interval second ejaculation in ICSI cycles. Results: Short-interval second ejaculation significantly improved sperm motility, normal morphological rate, and sperm DNA integrity both before and after sperm swim-up. The high-quality blastocyst rate (24.79% versus 14.67%), available blastocyst rate (57.56% versus 48.44%), and oocyte utilization rate (52.93% versus 45.29%) were significantly higher in the second ejaculation group (P<0.05). The clinical pregnancy rate (59.09% versus 47.37%), implantation rate (42.11% versus 32.35%) and live birth rate (40.91% versus 31.58%) were higher in the second ejaculation group, but the differences were not significant (P>0.05). Time-lapse analysis showed that morphokinetic time points after the 7-cell stage were earlier in the second ejaculation group but without a significant difference (P>0.05), and abnormal embryo cleavage patterns between the two groups were not significantly different (P>0.05). Conclusions: Short-interval second ejaculation significantly improves sperm quality in oligoasthenozoospermic males, and is beneficial for blastocyst formation efficiency in ICSI cycles. This study suggested a non-invasive and simple but effective strategy for improving ICSI treatment outcomes. [ABSTRACT FROM AUTHOR]

PURPOSE To assess the quantification accuracy of pulmonary nodules using virtual monoenergetic images (VMIs) derived from spectral-detector computed tomography (CT) under an ultra-low-dose scan protocol. METHODS A chest phantom consisting of 12 pulmonary nodules was scanned using spectral-detector CT at 100 kVp/10 mAs, 100 kVp/20 mAs, 120 kVp/10 mAs, and 120 kVp/30 mAs. Each scanning protocol was repeated three times. Each CT scan was reconstructed utilizing filtered back projection, hybrid iterative reconstruction, iterative model reconstruction (IMR), and VMIs of 40-100 keV. The signal-to-noise ratio and air noise of images, absolute differences, and absolute percentage measurement errors (APEs) of the diameter, density, and volume of the four scan protocols and ten reconstruction images were compared. RESULTS With each fixed reconstruction image, the four scanning protocols exhibited no significant differences in APEs for diameter and density (all P > 0.05). Of the four scan protocols and ten reconstruction images, APEs for nodule volume had no significant differences (all P > 0.05). At 100 kVp/10 mAs, APEs for density using IMR were the lowest (APE-mean: 6.69), but no significant difference was detected between VMIs at 50 keV (APE-mean: 11.69) and IMR (P = 0.666). In the subgroup analysis, at 100 kVp/10 mAs, there were no significant differences between VMIs at 50 keV and IMR in diameter and density (all P > 0.05). The radiation dose at 100 kVp/10 mAs was reduced by 77.8% compared with that at 120 kVp/30 mAs. CONCLUSION Compared with IMR, reconstruction at 100 kVp/10 mAs and 50 keV provides a more accurate quantification of pulmonary nodules, and the radiation dose is reduced by 77.8% compared with that at 120 kVp/30 mAs, demonstrating great potential for ultra-low-dose spectral-detector CT. [ABSTRACT FROM AUTHOR]

Objectives: To explore the value of radiomics based on Dual-energy CT (DECT) for discriminating preinvasive or MIA from IA appearing as GGNs before surgery. Methods: The retrospective study included 92 patients with lung adenocarcinoma comprising 30 IA and 62 preinvasive-MIA, which were further divided into a training (n=64) and a test set (n=28). Clinical and radiographic features along with quantitative parameters were recorded. Radiomics features were derived from virtual monoenergetic images (VMI), including 50kev and 150kev images. Intraclass correlation coefficients (ICCs), Pearson's correlation analysis and least absolute shrinkage and selection operator (LASSO) penalized logistic regression were conducted to eliminate unstable and redundant features. The performance of the models was evaluated by area under the curve (AUC) and the clinical utility was assessed using decision curve analysis (DCA). Results: The DECT-based radiomics model performed well with an AUC of 0.957 and 0.865 in the training and test set. The clinical-DECT model, comprising sex, age, tumor size, density, smoking, alcohol, effective atomic number, and normalized iodine concentration, had an AUC of 0.929 in the training and 0.719 in the test set. In addition, the radiomics model revealed a higher AUC value and a greater net benefit to patients than the clinical-DECT model. Conclusion: DECT-based radiomics features were valuable in predicting the invasiveness of GGNs, yielding a better predictive performance than the clinical-DECT model. [ABSTRACT FROM AUTHOR]

Dianthus caryophyllus is an economic species often considered excellent cut flowers and is suitable for bouquets and gardens. Here, we assembled the haplotype-resolved genome of D. caryophyllus 'Aili' at the chromosome level for the first time. The total lengths of the two assembled haplotypes of carnation were 584.88 Mb for haplotype genome 1 (hap1) and 578.78 Mb for haplotype genome 2 (hap2), respectively. We predicted a total of 44,098 and 42,425 protein-coding genes, respectively. The remarkable structure variation was identified between two haplotypes. Moreover, we identified 403.80 Mb of transposable elements (TEs) in hap1, which accounted for 69.34% of the genome. In contrast, hap2 had 402.70 Mb of TEs, representing 69.61% of the genome. Long terminal repeats were the predominant transposable elements. Phylogenetic analysis showed that the species differentiation time between carnation and gypsophila was estimated to be ~54.43 MYA. The unique gene families of carnation genomes were identified in 'Aili' and previously published 'Francesco' and 'Scarlet Queen'. The assembled and annotated haplotyperesolved D. caryophyllus genome not only promises to facilitate molecular biology studies but also contributes to genome-level evolutionary studies. [ABSTRACT FROM AUTHOR]

Objective: Malassezia furfur (M. furfur) is a lipophilic, conditionally pathogenic yeast that mainly causes skin infections, but the reports of related invasive infections are increasing. The aim of this study is to provide clinical data to assist physicians in the management of patients with invasive infections caused by M. furfur. Methods: A case of pulmonary infection caused by M. furfur in a hematopoietic stem cell transplant patient for aplastic anemia was reported. In addition, the literature on invasive infection by M. furfur published in PubMed and Web of Science in English until 31 July 2022 was reviewed. Results: Clinical data analysis of 86 patients (from 37 studies and our case) revealed that most of them were preterm (44.2%), followed by adults (31.4%). M. furfur fungemia occurred in 79.1% of the 86 patients, and 45 of them were clearly obtained from catheter blood. Other patients developed catheter-related infections, pneumonia, peripheral thromboembolism, endocarditis, meningitis, peritonitis and disseminated infections. Thirty-eight preterm infants had underlying diseases such as very low birth weight and/or multiple organ hypoplasia. The remaining patients had compromised immunity or severe gastrointestinal diseases. 97.7% of patients underwent invasive procedures and 80.2% received total parenteral nutrition (TPN). Fever, thrombocytopenia and leukocytosis accounted for 55.8%, 38.4% and 24.4% of patients with M. furfur invasive infections, respectively. 69.8% of the patients received antifungal therapy, mainly amphotericin B (AmB) or azoles. Of 84 patients with indwelling catheters, 58.3% underwent the removal of catheters. TPN were discontinued in 30 of 69 patients. The all-cause mortality of 86 patients was 27.9%. Conclusions: M. furfur can cause a variety of invasive infections. These patients mostly occur in premature infants, low immunity and severe gastrointestinal diseases. Indwelling catheters and TPN infusion are major risk factors. AmB, lAmB and azoles are the most commonly used agents, and simultaneous removal of the catheter and termination of TPN infusion are important for the treatment of M. furfur invasive infections. [ABSTRACT FROM AUTHOR]

Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen causing substantial financial losses in the global swine industry. The prevention of PRRSV is hampered due to frequent gene recombination among different strains of PRRSV. In this study, a new PRRSV strain, PRRSV-HQ-2020, was identified from nursery piglets in Yunnan Province, China, in 2020. The complete genome analysis revealed that PRRSV-HQ-2020 is highly similar to JXA1-like (lineage 8.7 PRRSV, isolated from China in 2008) in the 5'UTR, nsp1-9, and nsp11 coding regions. Additionally, it has a resemblance to JM (lineage 3 PRRSV, isolated from Taiwan, China, in 2010) in the nsp12-M coding region and NADC30 (lineage 1.8 PRRSV, isolated from North American in 2008) in the nsp10, N, and 3'UTR, suggesting a natural recombination event. Furthermore, recombination analyses showed three interlineage recombination events among lineages 8.7, 1.8, and 3. Notably, the GP5 protein of PRRSV-HQ-2020 exhibited a crucial mutation at position 44, leading to the deletion of a key glycosylation site. These findings provide direct evidence for the natural occurrence of recombination events among three lineages of PRRSV-2 in Chinese swine herds, leading to the emergence of unique genetic properties of PRRSV variants, and providing a theoretical basis for developing better PRRSV prevention strategies. [ABSTRACT FROM AUTHOR]

Despite a plethora of studies on practice of Content and Language Integrated Learning in most European countries, few have examined Content and Language Integrated Learning teacher training in China. This study aimed to examine the effectiveness of a Content and Language Integrated Learning teacher training platform for elementary English education in China. It sought to answer the following main research questions: What are the contents differences in Content and Language Integrated Learning teacher training in China? What are in-service English teachers' perceptions of Content and Language Integrated Learning after the training? And What are the factors which affect the training effect? The qualitative evidence showed that the training content of the teacher platform in China has been flexibly designed following the Chinese elementary English education context, but it also reflected the lack of consideration of teachers' individual needs in the design of the platform's training content and the lack of practical sessions and the long-term follow-up support. The statistical evidence showed that teaching experience significantly determines Content and Language Integrated Learning efficacy, and the educational background has little bearing on Content and Language Integrated Learning perceptions. Furthermore, all teachers from different educational backgrounds had a positive perception of their Content and Language Integrated Learning competence. However, Participants believed that their theoretical knowledge and teaching abilities are not equal, which suggests that instructors in this field urgently need help. Further research examining the cultivation of pre-service Content and Language Integrated Learning teachers would be worthy of investigation. [ABSTRACT FROM AUTHOR]

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Haploidentical hematopoietic stem cell transplantation (haplo-HSCT), as one of the life-saving treatments for severe aplastic anemia (SAA), is widely used because of its great donor availability. Over decades, granulocyte colony-stimulating factor (G-CSF)/antithymocyte globulin (ATG)-based protocol (the so-called Beijing Protocol) has achieved favorable engraftment and survival outcomes. In this study, we modified the conventional Beijing Protocol: the full-dose Cyclophosphamide (Cy) (200 mg/kg in total) was divided into 42.75 mg/kg Cy on day -5 to day -2 and Low dose post-transplant Cy (PTCy) (14.5 mg/kg on days +3 and +4), hoping to reduce the incidence of severe acute graft-versus-host disease (aGVHD) and to guarantee successful and stable engraftment. Here we retrospectively reported and analyzed the data of first 17 patients with SAA who had received haplo-HSCT using this novel regimen between August 2020 and August 2022. The median follow-up was 522 days (range, 138-859 days). No patient developed primary graft failure. Four (23.5%) patients developed grade II bladder toxicity, two (11.8%) patients developed grade II cardiotoxicity. All patients achieved neutrophil and platelet engraftment at median times of 12 days (range, 11–20 days) and14 days (range, 8-36 days). During our follow-up, no patients developed grade III-IV aGVHD. The cumulative incidence of grade II and grade I aGVHD at 100 days was 23.5% (95% CI, 6.8%-49.9%) and 47.1% (95% CI, 23.0%-72.2%). Three patients (17.6%) developed chronic GVHD of skin, mouth, and eyes and all of which were mild. All patients are alive by the end of the follow-up, with a failure-free survival of 100%, which was defined as survival without treatment failures, such as death, graft failure, or relapse rate. The rate of cytomegalovirus (CMV) reactivation was 82.4% (95% CI, 64.3%-100%). The rate of Epstein-Barr virus (EBV) reactivation was 17.6% (95% CI, 3.8%-43.4%). No CMV disease and post-transplantation lymphoproliferative disorder (PTLD) occurred among these patients. In conclusion, the encouraging results of prolonged survival outcomes and reduced incidence of GVHD suggest promising effect of this novel regimen in haplo-HSCT for patients with SAA. Larger-sample prospective clinical trials are needed to confirm the effectiveness of this regimen. [ABSTRACT FROM AUTHOR]

Background: Patients with gout carry an excess risk for cardiovascular disease (CVD), but the contribution of subclinical atherosclerosis to the CVD risk has never been reported. In this study, we aimed to explore the predictive factors for incident major adverse cardiovascular events (MACE) in gout patients without a previous history of CVD or cerebral vascular disease. Methods: A single-center, long-term follow-up cohort analysis was performed to assess subclinical atherosclerosis at baseline since 2008. Patients with a previous history of CVD or cerebrovascular disease were excluded. The outcome of the study was the first MACE. The presence of subclinical atherosclerosis was assessed by carotid plaque (CP), and carotid intima-media thickness (CMIT) was determined by ultrasound. An ultrasound scan of bilateral feet and ankles was performed at baseline. The association between tophi, carotid atherosclerosis, and the risk of developing incident MACE was evaluated using Cox proportional hazards models with adjustment for the CVD risk scores. Results: A total of 240 consecutive patients with primary gout were recruited. Their mean age was 44.0 years, with male predominance (238, 99.2%). During a median follow-up of 10.3 years, incident MACE was ascertained in 28 (11.7%) patients. In a Cox hazardsmodel, controlling for the CV risk scores, the presence of at least two tophi (HR, 2.12-5.25, p < 0.05) and carotid plaque (HR, 3.72-4.01, p < 0.05) were identified as independent predictors of incident MACE in gout patients. Conclusions: The presence of at least two tophi and carotid plaque on an ultrasound could independently predict MACE in addition to conventional cardiovascular risk factors in gout patients. [ABSTRACT FROM AUTHOR]

The lance is a critical component of the bottom-blowing pool melting process, and its placement has an important impact on the pool's gas-liquid two-phase flow. In this study, a mathematical model of the bottom-blowing process is established, and the flow pattern, trajectory, wake vortex, and velocity of bubbles under four lance spacings are simulated. Results show that there are three basic bubble flow patterns appear in the flow field: bubbles coalesce before leaving the nozzle (Pattern II), bubbles coalesce after leaving the nozzle (Pattern I), and no coalescence during the rise of bubbles (Pattern III). The bubble pattern varies from Pattern I to Pattern III with the increase in lance spacing. The intensity of the influence of the wake vortex on the bubbles decreases. The Q (the Q is the second Galilean invariant of the velocity gradient tensor ∇v) value of the wake vortex is small, but the vortex structural distribution is complex. Moreover, there is a large velocity difference between gas and liquid at the beginning of gas injection, but the velocity difference between them decreases after gas injection, so the average turbulent kinetic energy in the pool initially increases sharply, and then approaches dynamic equilibrium. The top and bottom velocities of the bubbles are consistent, and the velocity fluctuation is orderly. Moreover, the greater the lance spacing is, the greater the disturbance in the pool is. The mixing effect of D = 0.2 m is the best among the four spacings. [ABSTRACT FROM AUTHOR]

Background: Multiple surgical interventions exist for the treatment of symptomatic knee osteoarthritis, but the surgeon and patient may often have difficulty deciding which interventions are the best option. Methods: We conducted a systematic review to identify randomized clinical trials (RCTs) that compared complications, revisions, reoperations, and functional outcomes among TKA (total knee arthroplasty), UKA (unicompartmental knee arthroplasty), HTO (high tibial osteotomy), BCA (bicompartmental knee arthroplasty), BIU (bi-unicompartmental knee arthroplasty), and KJD (knee joint distraction). The PubMed, Embase, and Cochrane databases were reviewed for all studies comparing two or more surgical interventions. Direct-comparison meta-analysis and network meta-analysis (NMA) were performed to combine direct and indirect evidence. The risk of bias was assessed using the revised Cochrane risk of bias tool for RCTs. Results: This NMA and systematic review included 21 studies (17 RCTs), with a total of 1749 patients. The overall risk-of-bias assessment of the RCTs revealed that 7 studies had low risk, 5 had some concerns, and 9 had high risk. SUCRA (the surface under the cumulative ranking curve) rankings revealed that KJD had the greatest risk of appearing postoperative complications, revisions, and reoperations, and UKA or TKA had the lowest risk. The majority of comparisons among various treatments showed no difference for functional outcomes. Conclusion: Each surgical intervention is noninferior to other treatments in functional outcomes, but UKA and TKA are better options to treat OA according to SUCRA rankings by comparing complications, revisions, and reoperations. KJD is an imperfect option for treating OA. Other treatments should be carefully considered for each patient in accordance with their actual conditions. However, this conclusion is limited by the selection of reviewed publications and individual variation of surgical indications for patients. Trial registration: This study was registered with Research Registry (reviewregistry1395). [ABSTRACT FROM AUTHOR]

Mutations in the HERC2 and OCA2 genes have the potential to affect pigment deposition and alter feather color in birds. Therefore, in this study, we evaluated HERC2-OCA2 gene locus polymorphisms in Korean and Beijing white quails using RNA-Seq and KASP technology. The expression levels of HERC2 and OCA2 mRNA in skin tissues were analyzed using RT-qPCR. Ten single nucleotide polymorphisms were identified by RNA-Seq, of which three (n.117627564T>A, n.117674275T>G, n.117686226A>C) exhibited significant association with feather color in quail. The expression of OCA2 mRNA was significantly lower in the skin of Beijing white quails than that in the skin of Korean quails. These results suggested that variants in HERC2-OCA2 intergenic region could influence the expression of OCA2, which may underlie diluted feather color in the Beijing white quail. [ABSTRACT FROM AUTHOR]

This paper describes two new interim connection spaces ICSs), P-206 and P-3(DC, for spectral image compression and reconstruction. For this type of ICS, the weighting table or compression matrix H is modelled as a variable. The associated reconstruction matrix N is chosen as the Weiner estimation matrix. The objective function fCH) is the combination of the averages of the CIEDE2000 color difference (AEoo) and the root mean square error (RMSE) between the original and reconstructed reflectance values. Hence, the compression matrix H is determined by solving the nonlinear minimization problem based on the Munsell training dataset. The proposed ICSs, P-206 and P-306, were tested and compared, respectively, with ICS-2SI and ICS-3SI developed by Zhang et al. (JOSAA, Vol. 29, pp. 1027-1034) in 2012 using the NCS dataset, and 2 spectral images. Performance tests showed that the proposed P-206 and P-306 ICSs are better than the ICS-2SI and ICS-3SI ICSs, respectively, in terms of RMSE, goodness of fit coefficient (GFC), and AEoo under ClE illuminants [)65, A, C and F11. Therefore, it is expected that the P-206 and P-306 ICSs can find applications in spectral image compression and cross-media reproduction. [ABSTRACT FROM AUTHOR]

Introduction: Although geriatric assessment (GA) has been used for a long time in the field of geriatrics and internal medicine, there are few studies on its application in the field of breast surgery. Therefore, the utility of specific GA domains for the assessment of older patients with breast cancer remains unclear. The aim of the present study was to evaluate the association between specificGA domains and the survival rate of older patients with breast cancer. Methods: We used the database of Peking Union Medical College Hospital to identify older patients who were newly diagnosed with breast cancer between 2012 and 2018 and retrospectively analysed the data of 541 patients aged =65 years. Patients with metastatic cancer and those with missing vital status data were excluded. The primary outcomes were overall survival (OS) and breast cancer-specific survival. The GA domains used in this study included functional status, comorbidities, and psychological state. Multivariate regression analysis was used to estimate hazard ratios for these three domains. Results: After a median follow-up of 72 months, we observed a significant relationship between functional impairment and mortality (adjusted HR: 3.06, 95% confidence interval [CI]: 1.83-5.10, P<0.001). Similarly, patients with severe comorbidities (adjusted HR: 2.35; 95% CI: 1.16-4.75, P=0.017) and an impaired psychological state (adjusted HR: 2.82, 95% CI: 1.45-5.50, P=0.002) showed worse OS rates. Accordingly, addition of the three GA domains to the basic model, which included age, tumour stage, lymph node stage, and intrinsic molecular subtype as baseline variables, yielded higher C-statistics for mortality analysis (from 0.713 to 0.740). Conclusion: To our knowledge, this is the first study to include specific GA domains in a prognostic model for older patients with breast cancer in China. Three domains, namely functional status, comorbidities, and psychological state, should be considered for survival analyses in this particular population. The full model including these three GA domains may be more accurate in predicting the survival of older patients with breast cancer. [ABSTRACT FROM AUTHOR]

Background: AMKL without DS is a rare but aggressive hematological malignant disease in children, and it is associated with inferior outcomes. Several researchers have regarded pediatric AMKL without DS as high-risk or at least intermediate-risk AML and proposed that upfront allogenic hematopoietic stem cell transplantation (HSCT) in first complete remission might improve long-term survival. Patients and method: We conducted a retrospective study with twenty-five pediatric (< 14 years old) AMKL patients without DS who underwent haploidentical HSCT in the Peking University Institute of Hematology, Peking University People's Hospital from July 2016 to July 2021. The diagnostic criteria of AMKL without DS were adapted from the FAB and WHO: = 20% blasts in the bone marrow, and those blasts expressed at least one or more of the platelet glycoproteins: CD41, CD61, or CD42. AMKL with DS and therapy related AML was excluded. Children without a suitable closely HLA-matched related or unrelated donor (donors with more than nine out of 10 matching HLA-A, HLA-B, HLA-C, HLA-DR, and HLA-DQ loci), were eligible to receive haploidentical HSCT. Definition was adapted from international cooperation group. All statistical tests were conducted with SPSS v.24 and R v.3.6.3. Results: The 2-year OS was 54.5 ± 10.3%, and the EFS was 50.9 ± 10.2% in pediatric AMKL without DS undergoing haplo-HSCT. Statistically significantly better EFS was observed in patients with trisomy 19 than in patients without trisomy 19 (80 ± 12.6% and 33.3 ± 12.2%, respectively, P = 0.045), and OS was better in patients with trisomy 19 but with no statistical significance (P = 0.114). MRD negative pre-HSCT patients showed a better OS and EFS than those who were positive (P < 0.001 and P = 0.003, respectively). Eleven patients relapsed post HSCT. The median time to relapse post HSCT was 2.1 months (range: 1.0-14.4 months). The 2-year cumulative incidence of relapse (CIR) was 46.1 ± 11.6%. One patient developed bronchiolitis obliterans and respiratory failure and died at d + 98 post HSCT. Conclusion: AMKL without DS is a rare but aggressive hematological malignant disease in children, and it is associated with inferior outcomes. Trisomy 19 and MRD negative pre-HSCT might contribute to a better EFS and OS. Our TRM was low, haplo-HSCT might be an option for high-risk AMKL without DS. [ABSTRACT FROM AUTHOR]

Because of its high prevalence and poor long-term clinical treatment effect, liver disease is regarded as a major public health problem around the world. Among them, viral hepatitis, fatty liver, cirrhosis, non-alcoholic fatty liver disease (NAFLD), and autoimmune liver disease are common causes and inducements of liver injury, and play an important role in the occurrence and development of hepatocellular carcinoma (HCC). Tanshinone IIA (TsIIA) is a fat soluble polyphenol of Salvia miltiorrhiza that is extracted from Salvia miltiorrhiza. Because of its strong biological activity (anti-inflammatory, antioxidant), it is widely used in Asia to treat cardiovascular and liver diseases. In addition, TsIIA has shown significant anti-HCC activity in previous studies. It not only has significant anti proliferation and pro apoptotic properties. It can also play an anti-cancer role by mediating a variety of signal pathways, including phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt)/rapamycin (mTOR), mitogen-activated protein kinase (MAPK), and nuclear factor kappa-B (NF-kB). This review not only reviews the existing evidence and molecular mechanism of TsIIA's anti-HCC effect but also reviews the liverprotective effect of TsIIA and its impact on liver fibrosis, NAFLD, and other risk factors for liver cancer. In addition, we also conducted network pharmacological analysis on TsIIA and HCC to further screen and explore the possible targets of TsIIA against hepatocellular carcinoma. It is expected to provide a theoretical basis for the development of anti-HCC-related drugs based on TsIIA. [ABSTRACT FROM AUTHOR]

This paper deals with the asymptotic behavior of the solutions to a class of non-autonomous fractional stochastic p-Laplacian equation driven by linear additive noise on the entire space ℝn. We firstly prove the existence of a continuous non-autonomous cocycle for the equation as well as the uniform estimates of solutions. We then show pullback asymptotical compactness of solutions as well as the existence and uniqueness of tempered random attractors and the uniform tail-estimates of the solutions for large space variables when time is large enough to surmount the lack of compact Sobolev embeddings on unbounded domains. Finally, we establish the upper semi-continuity of the random attractors when noise intensity approaches zero. [ABSTRACT FROM AUTHOR]

In order to study the movement and shape change characteristic of bubble when passing through the interface of two kinds of liquids with different viscosity, the free rising process of a single bubble in static stratified liquids was numerically simulated with the volume-of-fluid method. The results show that, when the initial height of bubble rising is the same, the rising velocity, deformation increase with the increase of bubble radius. When the maximum intensity of the vortex in the bubble is distributed at the top of the bubble, the top of the left and right sides and the bottom of the left and right sides, the bubble shape is spherical, ellipsoid and spherical cap shape respectively. At different initial heights, the bubble trajectory shows three different shapes – linear, spiral, and C-shaped. The relationship between the bubble aspect ratio and rising height is predicted when different radius bubble passing through the interface. The amount of liquid B (lower layer) carried by the bubble increases with the increase of the bubble’s initial radius, and the amount of liquid carried by bubbles in C-shaped trajectory is higher than that in spiral trajectory. [ABSTRACT FROM AUTHOR]

Objective: To investigate a novel diagnostic model for benign and malignant pulmonary nodule diagnosis based on radiomic and clinical features, including urine energy metabolism index. Methods: A total of 107 pulmonary nodules were prospectively recruited and pathologically confirmed as malignant in 86 cases and benign in 21 cases. A chest CT scan and urine energy metabolism test were performed in all cases. A nomogram model was established in combination with radiomic and clinical features, including urine energy metabolism levels. The nomogram model was compared with the radiomic model and the clinical feature model alone to test its diagnostic validity, and receiver operating characteristic (ROC) curves were plotted to assess diagnostic validity. Results: The nomogram was established using a logistic regression algorithm to combine radiomic features and clinical characteristics including urine energy metabolism results. The predictive performance of the nomogram was evaluated using the area under the ROC and calibration curve, which showed the best performance, area under the curve (AUC) = 0.982, 95% CI = 0.940–1.000, compared to clinical and radiomic models in the testing cohort. The clinical benefit of the model was assessed using the decision curve analysis (DCA) and using the nomogram for benign and malignant pulmonary nodules, and preoperative prediction of benign and malignant pulmonary nodules using nomograms showed better clinical benefit. Conclusion: This study shows that a coupled model combining CT imaging features and clinical features (including urine energy metabolism) in combination with the nomogram model has higher diagnostic performance than the radiomic and clinical models alone, suggesting that the combination of both methods is more advantageous in identifying benign and malignant pulmonary nodules. [ABSTRACT FROM AUTHOR]

There are three main challenges to improving sclerotinia stem rot (SSR) resistance in rapeseed (Brassica napus L.). First, breeding materials such as the backbone parents have not been extensively investigated, making the findings of previous studies difficult to directly implement. Second, SSR resistance and flowering time (FT) loci are typically linked; thus, use of these loci requires sacrifice of the rapeseed growth period. Third, the SSR resistance loci in susceptible materials are often neglected, thereby reducing the richness of resistant resources. This study was conducted to investigate the stem resistance, disease index, and FT of a doubled haploid population consisting of 151 lines constructed from the backbone parent 19514A and conventional rapeseed cultivar ZY50 within multiple environments. Quantitative trait locus (QTL) mapping revealed 13 stem resistance QTLs, 9 disease index QTLs, and 20 FT QTLs. QTL meta-analysis showed that uqA04, uqC03.1, and uqC03.2 were repeatable SSR resistance QTLs derived from different parents but not affected by the FT. Based on these three QTLs, we proposed a strategy for improving the SSR resistance of 19514A and ZY50. This study improves the understanding of the resistance to rapeseed SSR and genetic basis of FT and demonstrates that SSR resistance QTLs can be mined from parents with a minimal resistance level difference, thereby supporting the application of backbone parents in related research and resistance improvement. [ABSTRACT FROM AUTHOR]