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Bacillus cereus is a foodborne opportunistic pathogen commonly found in humans and animals. It produces various toxins, causing frequent food safety incidents. Therefore, establishing a fast and accurate method for detecting B. cereus enterotoxin is crucial for disease diagnosis and food safety. In this study, Haemolysin BL comprising Hbl B and L2 was obtained from a prokaryotic expression system and then used to immunize mice for antibody preparation. Paired antibodies 2A10–5C7 against Hbl B and 1E2–10A4 against Hbl L2 were screened using the chessboard method and then used to construct a double-antibody sandwich detection method and a lateral flow immunochromatographic assay (LFIA) to quantify the concentrations of Hbl B and L2 in PBS and milk. The limits of detection for Hbl B and L2 in milk were 0.74 ng mL−1 and 1 ng mL−1 with detection ranges of 1.48–645.5 ng mL−1 and 2.33–391.5 ng mL−1. The spiked recoveries ranged from 82.2% to 105.67% and there was no cross-reactivity with common microbial toxins. The established LFIA was low in cost and rapid and was comparable with commercially available detection kits for food samples. [ABSTRACT FROM AUTHOR]

Purpose: An AI concierge is a technologically advanced, intelligent and personalized assistant that is designated to an individual customer, proactively taking care of that customer's needs throughout the service journey. This article envisions the idea of AI concierges and discusses how to leverage AI concierges in the customer journey. Design/methodology/approach: This article takes a conceptual approach and draws insights from literature in service management, marketing, psychology, human-computer interaction and ethics. Findings: This article delineates the fundamental forms of AI concierges: dialog interface (no embodiment), virtual avatar (embodiment in the virtual world), holographic projection (projection in the physical world) and tangible service robot (embodiment in the physical world). Key attributes of AI concierges are the ability to exhibit semantic understanding of auditory and visual inputs, maintain an emotional connection with the customer, demonstrate proactivity in refining the customer's experience and ensure omnipresence through continuous availability in various forms to attend to service throughout the customer journey. Furthermore, the article explores the multifaceted roles that AI concierges can play across the pre-encounter, encounter and post-encounter stages of the customer journey and explores the opportunities and challenges associated with AI concierges. Practical implications: This paper provides insights for professionals in hospitality, retail, travel, and healthcare on leveraging AI concierges to enhance the customer experience. By broadening AI concierge services, organizations can deliver personalized assistance and refined services across the entire customer journey. Originality/value: This article is the first to introduce the concept of the AI concierge. It offers a novel perspective by defining AI concierges' fundamental forms, key attributes and exploring their diverse roles in the customer journey. Additionally, it lays out a research agenda aimed at further advancing this domain. [ABSTRACT FROM AUTHOR]

Purpose: This study sought to understand which food allergen labeling systems (non-directive, semi-directive, and directive) were attended to and preferred by 34 participants with food hypersensitivity and their perceived corporate social responsibility (CSR) and behavioral intention towards a restaurant that identifies food allergens on menus. Design/methodology/approach: This study used an online survey with open-ended and ranking questions, combined with eye-tracking technology, to explore participants' visual attention and design preferences regarding four menus. This study utilized one-way repeated measures analysis of variance (RM-ANOVA) and heat maps to analyze participants' menu-reading behaviors. A content analysis of survey responses and a ranking analysis of menus were conducted to understand the reasons behind consumers' preferred menu designs. Findings: The advisory statement was not much attended to. Participants identified food allergen information significantly quicker with the directive labeling system (icons) than the other two systems, implying they were eye-catching. Semi-directive labeling system (red text) has lower visit count and was more preferred than two other systems; each labeling system has its strengths and limitations. Participants viewed restaurants that disclosed food allergen information on menus as socially responsible, and they would revisit those restaurants in the future. Originality/value: This study was one of the first to explore, through use of eye-tracking technology, which food allergen labeling systems were attended to by consumers with food hypersensitivity. The use of triangulation methods strengthened the credibility of the results. The study provided empirical data to restauranteurs in the US on the values of food allergen identification on restaurant menus, although it is voluntary. [ABSTRACT FROM AUTHOR]

Avian leukemia is an infectious tumorous disease of chickens caused by subgroup A of the avian leukemia virus (ALV-A), which mainly causes long-term viremia, slow growth, immune suppression, decreased production performance, multi-tissue tumors, and even death. The infection rate of this disease is very high in chicken herds in China, causing huge economic losses to the poultry industry every year. We successfully expressed the specific antigen protein of ALV (P27) through recombinant protein technology and screened a pair of highly sensitive monoclonal antibodies (mAbs) through mouse immunity, cell fusion, and antibody pairing. Based on this pair of antibodies, we established a dual antibody sandwich ELISA and gold nanoparticle immunochromatographic strip (AuNP-ICS) detection method. In addition, the parameters of the dual antibody sandwich ELISA and AuNP-ICS were optimized under different reaction conditions, which resulted in the minimum detection limits of 0.2 ng mL−1 and 1.53 ng ml−1, respectively. Commonly available ELISA and AuNP-ICS products on the market were compared, and we found that our established immune rapid chromatography had higher sensitivity. This established AuNP-ICS had no cross-reactivity with Influenza A (H1N1), Influenza A (H9N2), respiratory syncytial virus (RSV), varicella-zoster virus (VZV), Listeria monocytogenes listeriolysin (LLO), and Staphylococcal enterotoxin SED or SEC. Finally, the established AuNP-ICS was used to analyze 35 egg samples, and the results showed 5 positive samples and 30 negative samples. The AuNP-ICS rapid detection method established by our group had good specificity, high sensitivity, and convenience, and could be applied to the clinical sample detection of ALV-A. [ABSTRACT FROM AUTHOR]

Understanding how green hotels can gain marketing advantages could motivate hoteliers to adopt green practices during construction and operation. This study investigates the mediation role of perceived psychological benefits on consumers' environmental concerns and their willingness to pay a measurable premium for green-label hotels. Data gathered from 540 Chinese consumers revealed that a mismatch between the involvement of hotels and online travel agencies (OTAs) hampers the relationship between environmental concerns and perceived psychological benefits of booking green-label hotels on OTAs, further influencing consumers' willingness to pay a premium. This research uncovers an overlooked but crucial factor that reduces consumer satisfaction with the hotel-choice process and why it makes consumers willing to pay a premium for green-label hotels. Drawing from the findings, recommendations are also proffered for hoteliers to conceive and execute green marketing tactics. [ABSTRACT FROM AUTHOR]

Norovirus is one of the main pathogens causing acute gastroenteritis worldwide, and possesses rich genetic diversity. We established a simple, fast, and suitable colloidal gold immunochromatographic detection method for common epidemic strains of Norovirus. A pair of monoclonal antibodies that are sensitive to recognizing Norovirus was successfully screened and used as gold-labeled antibody and capture antibody, respectively. The assembly conditions of the test strip were optimized to determine the labeling pH, the concentration of capture antibody and concentration of AuNP-labeled antibody, then the performance of the strips, including sensitivity testing and specificity testing, was evaluated. The minimum detection concentration of the established Norovirus colloidal gold test strip detection method is 10 ng mL−1. This antibody has no cross-reactivity with HCoV-NL63, HCoV-OC43, Influenza A (H1N1), Influenza A (H9N2), respiratory syncytial virus or Varicella zoster virus. The established colloidal gold detection method was used to analyze 11 fecal samples, and the results showed that only two samples were positive and the rest were negative, in agreement with the RT-PCR result. The whole colloidal gold test strip detection method process took 15 min, and could be used for on-site detection. The established colloidal gold test strip detection method has good specificity and stability, and can be used for the detection of common Norovirus strains and in large-scale epidemiological investigations. [ABSTRACT FROM AUTHOR]

Purpose: Building on the broaden-and-build theory and incorporating a self-regulatory perspective, this study examines the relationship between trait gratitude and subjective career success and investigates the mediating roles of growth mindset of work and career network breadth. Design/methodology/approach: Time-lagged data were collected in three waves from a sample of 314 employees in China. Hypotheses were tested using structural equation modeling. Findings: The findings demonstrate that trait gratitude is positively related to SCS, mediated by growth mindset of work as an indicator of psychological resources and career network breadth as an indicator of social resources. Trait gratitude is more strongly associated with network breadth (i.e., social resources) than with growth mindset (i.e., psychological resources). Practical implications: Organizations may find trait gratitude an applicable addition to the selection criteria during the recruitment process. Originality/value: By identifying trait gratitude as an antecedent of SCS and revealing its underlying mechanisms, the current study points to a new perspective on the study of career success. [ABSTRACT FROM AUTHOR]

This study examined the roles of perceived preventive measures and brand trust on the intention to dine out at restaurants during the reopening period in the United States. A total of 587 participants, recruited through a market research company completed the data. Multiple regression was used for data analysis. The results indicated that perceived importance of preventive measures enhanced customers' intention to dine out via brand trust. Perceived risk moderated the relationship between perceived importance of preventive measures and brand trust. The study provided significant implications for restaurant operation during the reopening period. [ABSTRACT FROM AUTHOR]

Purpose: This study aims to systematically review the work–family enrichment (WFE) studies in hospitality and tourism management and provide insight into the patterns and trends of WFE literature to practitioners and future researchers. Design/methodology/approach: This study design followed the guidelines of preferred reporting items of systematic reviews and meta-analysis. A final sample of 23 studies related to WFE that published from 2000 to 2021 was selected and systematically analyzed. Findings: This study resulted in a framework that the antecedents and outcomes of WFE at the organizational, job and individual levels. Moderators between antecedents and outcomes were also identified. Theories such as conservation of resources theory, boundary theory, role theory and expansion theory were frequently applied in the investigation of WFE. Research limitations/implications: This study generated a framework that illustrates the organization characteristics, the job characteristics and individual factors that have examined in hospitality literature on the topic of WFE. This study also pointed out theories that have been used in investigating WFE. However, this systematic review may subject to publication bias and number of reviewed articles remained relatively small. Practical implications: This study provides suggestions on how hospitality operators could use internal marketing strategies and certain leadership styles (e.g. transformational and servant leaderships) to enhance employees' WFE. Originality/value: This study summarized the publication trends and patterns of the WFE studies in hospitality and tourism management in the past two decades, which provides suggestions for future scholars to further explore this research topic. [ABSTRACT FROM AUTHOR]