1. Identification of Linear Epitopes in the C-Terminal Region of ASFV p72 Protein.
- Author
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Hu, Yifan, Wang, Anchen, Yan, Wanwan, Li, Junbo, Meng, Xin, Chen, Lingchao, Li, Songnan, Tong, Wu, Kong, Ning, Yu, Lingxue, Yu, Hai, Shan, Tongling, Xu, Jiaping, Tong, Guangzhi, and Zheng, Hao
- Subjects
AFRICAN swine fever virus ,MONOCLONAL antibodies ,EPITOPES ,AFRICAN swine fever ,WILD boar ,SWINE ,SWINE farms - Abstract
African swine fever, which is induced by the African swine fever virus (ASFV), poses a significant threat to the global pig industry due to its high lethality in domestic pigs and wild boars. Despite the severity of the disease, there is a lack of effective vaccines and drugs against the ASFV. The p72 protein, constituting 31 to 33% of the total virus particle mass, serves as the primary capsid protein of ASFV. It is a crucial antigen for the development of ASF subunit vaccines and serological diagnostic methods. In this investigation, 27 monoclonal antibodies (mAbs) were generated through mouse immunization with the truncated C-terminal p72 protein expressed by Escherichia coli. Among these, six mAbs exhibited binding to the p72 trimer, with their respective recognized epitopes identified as
542 VTAHGINLIDKF553 ,568 GNAIKTP574 , and584 FALKPREEY592 . All three epitopes were situated within the interval sequences of functional units of the C-terminal jelly-roll barrel of p72. Notably, two epitopes,568 GNAIKTP574 and584 FALKPREEY592 , were internal to the p72 trimer, while the epitope542 VTAHGINLIDKF553 was exposed on the surface of the trimer and consistently conserved across all ASFV genotypes. These findings enhance our comprehension of the antigenic function and structure of the p72 protein, facilitating the utilization of p72 in the development of diagnostic techniques for ASFV. [ABSTRACT FROM AUTHOR]- Published
- 2023
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