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The article presents information on the history of the Environmental Protection Agency (EPA). The EPA was aware that water and sewer services are essential for neighborhood livability and understood the significant costs associated with their upkeep and development. Yet, despite recognizing that its grant program had major implications for racial segregation in land use, the EPA treated civil rights as optional rather than mandatory. The author states that this approach mirrored the evasion of responsibility seen in other federal agencies, as highlighted by Christopher Bonastia, who noted that civil rights often lack meaningful enforcement when they become politically or economically costly. The EPA’s inaction, despite knowing the harm caused by its grants, underscores the deep connections between environmental protection and structural racism.

Bovine respiratory disease (BRD) contributes to high rates of mortality and morbidity among feedlot animals. Thus, there is a need to discover robust predictive biological indicators of infection status and disease severity in feedlot cattle. This study aimed to explore the use of 1 H nuclear magnetic resonance (NMR) based metabolite profiling to characterize serum molecular markers associated with a viralbacterial respiratory disease challenge in beef cattle. Crossbred beef steers [n = 24; initial body weight (BW) = 293 kg) were inoculated intranasally with bovine herpes virus-1 (2×108 PFU) and intratracheally with Mannheimia haemolytica (MH, 2.15×1010 CFU) on d -3 and 0, respectively (n = 16; MH-challenge), or similarly inoculated with phosphate-buffered saline (n = 8; Control-challenge). Blood was collected via jugular venipuncture on d -3, -1, 0, 2, 3, 5, 7, 10, and 14 relative to MH challenge for complete blood count and haptoglobin analysis, with additional serum samples stored at -80°C for subsequent metabolomic analysis. Continuously recorded variables included rumen temperature, activity, rumination, dry matter intake, and feeding behavior. Wottlin et al. (2020) reported that rumen temperature was increased (P < 0.04) in MH-challenged steers until d 6 post-MH inoculation (40.8 vs 39.3°C in MH-challenged and Controlchallenged steers several hours post-MH inoculation). Dry matter intake was reduced (P < 0.01) by 42% in MH-challenged compared with Control-challenged steers during the 14-d post-MH inoculation period. Further, haptoglobin concentrations were increased (P < 0.05) in MH-challenged steers from d 2 to 7 of the post-MH inoculation period. Serum samples underwent small molecule metabolite extraction and untargeted ¹ H NMR metabolomics. The ¹ H NMR spectra of serum metabolites were evaluated for this study, resulting in the unambiguous identification and quantification of 64 polar metabolites. Univariate analysis was conducted to examine the effect of the MH challenge on relative metabolite concentrations between d 2 and 5 relative to MH challenge, indicating differences between metabolites of MH-challenge and Control-challenged steers. However, multivariate analysis using unsupervised 2-dimensional principal component analysis (2D-PCA) could not differentiate MH-challenged from Control-challenged steers based on distinct serum metabolite profiles. Results indicate the potential of specific metabolites to indicate BRD infected cattle; however, a larger cohort of cattle and expanded metabolite profile coverage may be required to more fully differentiate between BRD vs healthy cohorts. Opportunities exist to further investigate the role of metabolomics to discover robust early biological indicators that are predictive of the onset of BRD in feedlot cattle. [ABSTRACT FROM AUTHOR]

Simple Summary: The eating experience of beef is strongly influenced by the amount of marbling or intramuscular fat. Beef steers are fed to a desired level of fatness in order to maximize the probability of a positive eating experience. This work aims to study the genes expressed in muscle and adipose tissue as beef steers deposit body fat to better understand the regulation of this important process and to more effectively predict positive eating experiences. This research identified genes that are altered as beef animals deposit fat in both the muscle and adipose tissues and the processes that are being altered, including the metabolism of fat and energy. The work also identified genes related to inflammation and injury in the adipose tissue that warrant additional research to further understand their role in beef animals. Targets for finished livestock are often determined by expected fat, either subcutaneous or intramuscular. These targets are used frequently to improve eating quality. Lower intramuscular fat, lack of product uniformity, and insufficient tenderness can negatively impact beef acceptability. This study aimed to investigate the differences in gene expression that alter metabolism and intercellular signaling in the muscle and adipose tissue in beef carcasses at different fat endpoints. In this study, longissimus thoracis muscle samples and adipose tissue were collected at harvest, and RNA was extracted and then sequenced using RNAseq. Differential expression was determined using edgeR, and p-values were adjusted using the Benjamini–Hochberg method. A corrected p-value of 0.005 and log2 (fold change) of >1 were the threshold to identify differential expression. Comparison between intermuscular and subcutaneous fat showed no differences in the genes activated in the two adipose tissue depots, suggesting that subcutaneous fat was an adequate sample. Carcass data allowed the classification of carcasses by USDA quality grades (marbling targets). In comparing muscle from Standard and Choice carcasses, 15 genes were downregulated, and 20 were upregulated. There were 49 downregulated and 113 upregulated genes comparing adipose tissue from Standard and Choice carcasses. These genes are related to the metabolism of fat and energy. This indicates that muscle transcript expression varies less than adipose. In addition, subcutaneous fat can be used to evaluate transcript changes in fat. However, it is unclear whether these fat tissues can be used as surrogates for marbling. [ABSTRACT FROM AUTHOR]

Graff and Korolczuk largely stress the latter, showing ways in which anti-gender politics have been "expedient for [the] political agenda" (83) to populist politicians, and arguing that there is an "opportunistic synergy" between anti-gender movements and populist right-wing parties, "with both sides benefitting" (24). Instead, it is fundamentally linked to not only ideology but these states' attempts to reconfigure state welfare and state-citizen relations. Fodor sets out to explain how the current Hungarian state welfare structure "carries some elements of both institutionalized gender relations in Western neoliberal democracies and the Central European state socialist gender regimes of the recent past" yet is also "distinctly different from both" (2). This is particularly central to Fodor's work, which argues that anti-gender politics are not just rhetoric but rather tangible and dramatic changes to state-citizen relations and the funding of social welfare. [Extracted from the article]

Almost a decade after Sweden first declared that it would follow a feminist foreign policy (FFP), a further eleven countries from across Europe, North and South America, and North and West Africa have adopted, or have signaled an interest in potentially adopting, an FFP in the future. These developments have been accompanied by a growing body of feminist scholarship. Although still in its infancy, this literature can generally be divided between more normative accounts and those that are empirically focused, with particular attention paid to the FFPs of Sweden and Canada. Yet, few studies compare FFPs' uptake across different countries and regions, examine its connections to longer histories of ideas around women and gender, or unpack the policy intersections FFP (tentatively) engages. Contributing to these different areas, Part I provides an overview of the history of FFP, interrogates FFP in the context of Foreign Policy Analysis, and explores what FFP can achieve in the current (liberal) global system. Part II turns to consider policy intersections in relation to the climate crisis, migration, militarism, and bodies. Thinking through its origins, policy intersections, and potential future(s), the contributors to this Forum explore FFP's multiple and contested future(s). Ultimately, the Forum takes stock of this feminist turn in foreign policy at a critical point in its development and considers what future possibilities it may hold. [ABSTRACT FROM AUTHOR]

In recent years, Brazil and Poland have elected governments that are sceptical of both the liberal international order and gender. In both cases, contemporary administrations have bolstered the pre-existing anti-gender offensive of religious and secular conservative forces and converted this into legislation and public policy. Yet, at the same time, both have also created National Action Plans around the UN's Women, Peace and Security agenda. Why is this the case? Why do two governments that see gender as an 'ideology' continue to work on WPS? Using a feminist institutionalist framework, this article draws on content analysis of the NAPs and semi-structured in-depth interviews with stakeholders in Brazil and Poland to explore this puzzle. We argue that the WPS agenda has survived in these political contexts due the presence of key 'femocrats' within the state; the influence of international institutions; and the symbolic power that the WPS agenda gives to these countries on the world stage. As such, the article makes a key contribution to the literature on the WPS agenda and also bolsters the argument for a complication of the idea of gender 'backlash' – in domestic and international audiences, states are willing to adopt different attitudes to gender if it furthers their interests. [ABSTRACT FROM AUTHOR]

Simple Summary: Animal fat deposition has a major impact on the meat yield from individual carcasses as well the perceived eating quality for consumers. Understanding the impact of livestock production practices on fat deposition and the molecular mechanisms activated will lead to a better understanding of finishing livestock. This enhanced understanding will also lead to the increased efficiency and improved sustainability of practices for livestock production. The impact of fat storage on physiological functions and health are also important. This review brings together both the production practices and the current understanding of molecular processes associated with fat deposition. Growth is frequently described as weight gain over time. Researchers have used this information in equations to predict carcass composition and estimate fat deposition. Diet, species, breed, and gender all influence fat deposition. Alterations in diets result in changes in fat deposition as well as the fatty acid profile of meat. Additionally, the amount and composition of the fat can affect lipid stability and flavor development upon cooking. Fat functions not only as a storage of energy and contributor of flavor compounds, but also participates in signaling that affects many aspects of the physiological functions of the animal. Transcription factors that are upregulated in response to excess energy to be stored are an important avenue of research to improve the understanding of fat deposition and thus, the efficiency of production. Additionally, further study of the inflammation associated with increased fat depots may lead to a better understanding of finishing animals, production efficiency, and overall health. [ABSTRACT FROM AUTHOR]

Understanding the influence of population attributes on genetic diversity is important to advancement of biological conservation. Because bighorn sheep (Ovis canadensis) populations vary in size and management history, the species provides a unique opportunity to observe the response of average pairwise kinship, inversely related to genetic diversity, to a spectrum of natural and management influences. We estimated average pairwise kinship of bighorn sheep herds and compared estimates with population origin (native/indigenous/extant or reintroduced), historical minimum count, connectivity, and augmentation history, to determine which predictors were the most important. We evaluated 488 bighorn sheep from 19 wild populations with past minimum counts of 16–562 animals, including native and reintroduced populations that received 0–165 animals in augmentations. Using the Illumina High Density Ovine array, we generated a dataset of 7728 single nucleotide polymorphisms and calculated average pairwise kinship for each population. Multiple linear regression analysis determined that connectivity between populations via dispersal, greater number of animals received in augmentations, and greater minimum count were correlated with lower average pairwise kinship at the population level, and whether the population was extant or reintroduced was less important. Thus, our results indicated that genetic isolation of populations can result in increased levels of inbreeding. By determining that natural and human‐assisted gene flow were likely the most important influences of average pairwise kinship at the population level, this study can serve as a benchmark for future management of bighorn sheep populations and aid in identifying populations of genetic concern to define priorities for conservation of wild populations. [ABSTRACT FROM AUTHOR]

The article focuses on study on comparative approach to refine molecular mechanism impacting carcass characteristics and meat quality. Topics discussed include way to raise livestock and produce product for food chain, differences in differentially expressed genes (DEGs), and accepted genomes utilized by Novogene.

Environmental metabolomics has the potential to facilitate the establishment of a new suite of tools for assessing the physiological status of important wildlife species. A first step in developing such tools is to evaluate the impacts of various capture techniques on metabolic profiles as capture is necessary to obtain the biological samples required for assays. This study employed 1H nuclear magnetic resonance (NMR)-based metabolite profiling of 562 blood serum samples from wild bighorn sheep to identify characteristic molecular serum makers of three capture techniques (dart, dropnet, and helicopter-based captures) to inform future sampling protocols for metabolomics studies, and to provide insights into the physiological impacts of capture. We found that different capture techniques induce distinct changes in amino acid serum profiles, the urea cycle, and glycolysis, and attribute the differences in metabolic patterns to differences in physical activity and stress caused by the different capture methods. These results suggest that when designing experiments involving the capture of wild animals, it may be prudent to employ a single capture technique to reduce confounding factors. Our results also supports administration of tranquilizers as soon as animals are restrained to mitigate short-term physiological and metabolic responses when using pursuit and physical restraint capture techniques. [ABSTRACT FROM AUTHOR]

Valuing Clean Air: The EPA and the Economics of Environmental Protection By Charles Halvorson. Rather than retreading a mythic narrative hinging on the Reagan administration's evisceration of the do-gooder agency, Halvorson shows how the agency was internally fragmented in the 1970s, as well as how the Reagan administration was criticized by the Republican Party for cudgeling the EPA's budget. [Extracted from the article]

This introduction provides an overview of the gendered nature of politics and international relations, before a brief summary of the articles that make-up this special issue. [ABSTRACT FROM AUTHOR]

Indicator traits associated with disease resiliency would be useful to improve the health and welfare of feedlot cattle. A post hoc analysis of data collected previously (Kayser et al., 2019a) was conducted to investigate differences in immunologic, physiologic, and behavioral responses of steers (N = 36, initial BW = 386 ± 24 kg) that had differential haptoglobin (HPT) responses to an experimentally induced challenge with Mannheimia haemolytica (MH). Rumen temperature, DMI, and feeding behavior data were collected continuously, and serial blood samples were collected following the MH challenge. Retrospectively, it was determined that 9 of the 18 MH-challenged steers mounted a minimal HPT response, despite having similar leukocyte and temperature responses to other MH-challenged steers with a greater HPT response. Our objective was to examine differences in behavioral and physiological responses between MH-challenged HPT responsive (RES; n = 9), MH-challenged HPT nonresponsive (NON; n = 9), and phosphate-buffered saline-inoculated controls (CON; n = 18). Additionally, 1H NMR analysis was conducted to determine whether the HPT responsive phenotype affected serum metabolite profiles. The RES steers had lesser (P < 0.05) cortisol concentrations than NON and CON steers. The magnitude of the increases in neutrophil concentrations and rumen temperature, and the reduction in DMI following the MH challenge were greatest (P < 0.05) in RES steers. Univariate analysis of serum metabolites indicated differences between RES, NON, and CON steers following the MH challenge; however, multivariate analysis revealed no difference between HPT-responsive phenotypes. Prior to the MH challenge, RES steers had longer (P < 0.05) head down and bunk visit durations, slower eating rates (P < 0.01) and greater (P < 0.05) daily variances in bunk visit frequency and head down duration compared with NON steers, suggesting that feeding behavior patterns were associated with the HPT-responsive phenotype. During the 28-d post challenge period, RES steers had decreased (P < 0.05) final BW, tended (P = 0.06) to have lesser DMI, and had greater (P < 0.05) daily variances in head down and bunk visit durations compared with NON steers, which may have been attributed to their greater acute-phase protein response to the MH challenge. These results indicate that the HPT-responsive phenotype affected feeding behavior patterns and may be associated with disease resiliency in beef cattle. [ABSTRACT FROM AUTHOR]

Wildlife restoration often involves translocation efforts to reintroduce species and supplement small, fragmented populations. We examined the genomic consequences of bighorn sheep (Ovis canadensis) translocations and population isolation to enhance understanding of evolutionary processes that affect population genetics and inform future restoration strategies. We conducted a population genomic analysis of 511 bighorn sheep from 17 areas, including native and reintroduced populations that received 0–10 translocations. Using the Illumina High Density Ovine array, we generated datasets of 6,155 to 33,289 single nucleotide polymorphisms and completed clustering, population tree, and kinship analyses. Our analyses determined that natural gene flow did not occur between most populations, including two pairs of native herds that had past connectivity. We synthesized genomic evidence across analyses to evaluate 24 different translocation events and detected eight successful reintroductions (i.e., lack of signal for recolonization from nearby populations) and five successful augmentations (i.e., reproductive success of translocated individuals) based on genetic similarity with the source populations. A single native population founded six of the reintroduced herds, suggesting that environmental conditions did not need to match for populations to persist following reintroduction. Augmentations consisting of 18–57 animals including males and females succeeded, whereas augmentations of two males did not result in a detectable genetic signature. Our results provide insight on genomic distinctiveness of native and reintroduced herds, information on the relative success of reintroduction and augmentation efforts and their associated attributes, and guidance to enhance genetic contribution of augmentations and reintroductions to aid in bighorn sheep restoration. [ABSTRACT FROM AUTHOR]

The article estimates the phenotypic differences, variance components of and among traits and the genetic and inbreeding trends in divergently selected Rambouillet lines for over 50 years. Topics discussed include selection of ram and ewe replacements, number of live or dead lambs at birth per ewe lambing, and dam age effects on prolificacy.

Across politics and public discourse, feminism is experiencing a global renaissance. Yet feminist academic work is divided over the burgeoning use of the term, particularly in reference to economic and international development policy. For some, feminism has been co-opted for neoliberal economic ends; for others, it remains a critical force across the globe. This article explores the nascent feminist foreign policies of Sweden and Canada. Employing a discourse analysis of both states' policy documents, it asks what the term "feminist" meant in preliminary attempts at constructing a feminist foreign policy. It argues that although both use the term "feminist," they understand the term very differently, with Sweden centering it in domestic and international commitments to change, while Canada places greater emphasis on the private sector. This suggests that this policy agenda is still developing its central concepts, and is thus ripe for intervention on the part of policymakers and civil society organizations. [ABSTRACT FROM AUTHOR]

Purpose: Venous thromboembolism (VTE) is a potentially life-threatening condition associated with both orthopaedic surgery and tumour growth. In this study, we identify risk factors associated with VTE in patients with musculoskeletal tumours using two national datasets. Methods: The ACS-NSQIP and NIS databases were queried for patients undergoing surgery with a diagnosis of benign or malignant musculoskeletal tumours. Chi-square and binary logistic regression analyses were used to determine risk factors for VTE. Results: The incidence of VTE was 2% in both databases. Patients with malignant tumours, those with tumours of the pelvis, sacrum, or coccyx, obesity, arrhythmias, paralysis, metastatic disease, coagulopathy, and recent weight loss were at increased risk for VTE. In patients with benign tumours, those who were African American, those with tumours of the pelvis, sacrum, or coccyx, diabetes, anaemia, and coagulopathy were at increased risk of VTE. Conclusions: Patients with malignant or benign lesion are at greater risk of VTE if they are age 30 and over, of the African American population, or with tumors of the pelvis/sacrum/coccyx, or any of the following comorbidities: pulmonary disease, paralysis, other neurological disorders, or coagulopathy. [ABSTRACT FROM AUTHOR]

Nationalism has long been understood to be a deeply gendered phenomenon. This article provides an overview of some of the key concepts and literature in the study of gender and nationalism, including women; gender; the nation and the intersection of sexuality, race, and migration; and gender within nationalist imaginations. It offers some future research agendas that might be pursued in work on gender and nationalism—namely the gendered dimensions of populism or "new" nationalism. [ABSTRACT FROM AUTHOR]

Since the inception of United Nations Security Council Resolution 1325 (UNSCR 1325) in 2000, feminist academia has been closely interested in the developing women, peace, and security (WPS) agenda in international affairs. The majority of this work has emerged from within feminist international relations (Mcleod 2015 ; Shepherd 2008) and feminist legal studies. Less attention has been paid to the WPS agenda by feminist political science. As a result, less consideration has been given to political institutions within the WPS framework. This paper argues that the design and implementation of postconflict political institutions is an important component of the WPS agenda and one which deserves greater attention. It demonstrates that using certain tenets of feminist political science, and feminist institutionalism in particular, can offer key insights into greater understanding of the importance of political institutions within postconflict societies. The article illustrates how political institutions have been underconsidered within academic work on the WPS agenda. It then argues that political institutions are an important part of the puzzle when it comes to implementing the WPS agenda. It shows how feminist institutional theory can help to provide key insights into the nature of postconflict institutions. [ABSTRACT FROM AUTHOR]

Rhabdomyosarcoma is the most common soft‐tissue sarcoma in children and adolescents and accounts for 3% of all pediatric tumors. Subtypes include alveolar, spindle cell, embryonal, mixed‐type, pleomorphic, and rhabdomyosarcoma with ganglionic differentiation. The National Cancer Institute's Surveillance, Epidemiology, and End Results (SEER) database was queried for patients diagnosed with any type of rhabdomyosarcoma between 1973 and 2014. Patient demographics, tumor characteristics, and incidence were studied with χ2 analysis. Survival was modeled with Kaplan–Meier survival curves and Cox proportional hazards models were used to assess the effect of age and gender on survival. Pleomorphic subtype had higher grade and larger sized tumors compared to other subtypes (p < 0.05). Pleomorphic and alveolar rhabdomyosarcoma had the worst overall survival with a 26.6% and 28.9% 5‐year survival, respectively. Embryonal rhabdomyosarcoma had the highest 5‐year survival rate (73.9%). Tumor size was negatively correlated with survival months, indicating patients with larger tumors had shorter survival times (p < 0.05). Presence of higher‐grade tumors and metastatic disease at presentation were negatively correlated with survival months (p < 0.05). No significant differences in the survival were found between gender or race between all of the subtypes (p > 0.05). This study highlights key differences in the demographic and survival rates of the different types of rhabdomyosarcoma that can be used for more tailored patient counseling. We also demonstrate that large, population‐level databases provide sufficient data that can be used in the analysis of rare tumors. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2226–2230, 2019 [ABSTRACT FROM AUTHOR]

Interest in beef cattle temperament has increased due to growing consumer awareness of animal welfare and increased concern for handler safety. Temperament measures are based on behavioral responses to a perceived stressor. Subjective chute scoring has been used to give a numeric value to temperament; however, the subjectivity and variability among observers have been questioned. To deal with the perceived subjectivity and variability, other researchers have used exit velocity. Researchers have related faster exit velocities to increased cortisol and plasma lactate. The objectives of this study were to compare temperament between feedlot steers and heifers and to confirm chute side measures of temperament relationship to physiological responses to stress. Body temperature, blood and plasma lactate, serum glucose, salivary and serum cortisol concentrations were measured on Bos taurus commercial crossbred feedlot cattle (n = 197). Fast, medium, and slow classifications were developed from exit velocities. Plasma lactate was significantly different between all exit velocity classes. Exit velocity and physiological measures indicated that heifers were more excitable (faster exit velocities (P = 0.003), higher plasma lactate concentrations (P = 0.03), and cortisol concentrations (P = 0.001)). Simple correlations among these variables indicated body temperature (heifers r = 0.44, P < 0.0001; steers r = 0.45, P <.0001), plasma lactate (heifers r = 0.52, P < 0.0001; steers r = 0.63, P < 0.0001), blood lactate (heifers r = 0.53, P < 0.001; steers r = 0.59, P < 0.001), and glucose (heifers r = 0.54, P < 0.001; steers r = 0.32, P <0.003) were all related to exit velocity. Cortisol measures were not correlated to exit velocity in steers but were in heifers. Linear models constructed and evaluated using the Akaike information criterion indicated that blood lactate in combination with rectal temperature were strong candidates to predict exit velocity. Using the discriminate function analysis, the model correctly categorized fast and slow classifications 69.23% and 61.54%, respectively, indicating that in combination measures of body temperature and blood lactate can potentially increase accuracy of temperament identification or replace exit velocity as a measure of temperament. The plasma lactate and rectal temperature have the potential to become strong objective measures to augment or replace exit velocity. [ABSTRACT FROM AUTHOR]

2018 marks the twentieth anniversary of the Good Friday/Belfast Agreement and the establishment of devolved governance in Northern Ireland. Yet, whilst devolution has largely been held to have positive effects in Scotland and Wales with regards to both women's descriptive and substantive representation, this impact has been less discernible in Northern Ireland. Of the four regions of the United Kingdom, politics in Northern Ireland is arguably the most unfeminised—women have routinely seen lower descriptive representation in the Northern Irish Assembly and policy-making in areas such as reproductive rights lies far behind the rest of the UK. The article explores why politics is so unfeminised in the post-conflict context in Northern Ireland, by looking at efforts to feminise formal politics (especially the various peace/inter-party agreements and attempts to include women in formal politics) and efforts to politicise feminist activism (the work of the women's sector to influence policy-making in the province). It then explores some of the academic explanations as to why the feminisation of politics remains so difficult in Northern Ireland. [ABSTRACT FROM AUTHOR]

Summary: Introduction: Despite increasing popularity of platelet‐rich plasma (PRP) in treating aging facial skin, the quality of evidence supporting its use is poor due to the lack of consistent methods of its preparation and application. Objective: This study was conducted to assess treatment efficacy and patient satisfaction with a single PRP treatment prepared with a simplified preparation and application technique. Methods: Four millilitre of PRP were injected into 6 standardized points on each side of the face. Outcomes were assessed by independent physician evaluation of pretreatment and posttreatment photographs using the Wrinkle Severity Rating Scale (WSRS) and Global Aesthetic Improvement Scale (GAIS). In addition, patient‐reported outcomes were evaluated using the FACE‐Q. Results: Thirty‐one participants ranging from 27 to 71 years of age (median, 38; IQR 32‐58) were recruited for this study. Posttreatment WSRS scores improved in only 1 patient; the GAIS scores of 14 patients indicated aesthetic improvement. Analysis of FACE‐Q scores revealed statistically significant increases in participant satisfaction with overall facial appearance and cheeks. The most frequently reported adverse effects were tenderness (23.4%; 7 of 31), facial tightness (20.0%; 6 of 31), and swelling (20.0%; 6 of 31). Conclusions: A simple method of PRP preparation offers modest benefit in treating the effects of skin aging and photodamage. Future research studies should alter our methods using a stepwise approach to optimize the treatment of aging facial skin with PRP. [ABSTRACT FROM AUTHOR]

Introduction: The aim of this study was to investigate complication rates and types following allograft reconstruction and discuss unique considerations for management. Methods: Seventy-four consecutive patients underwent large segment allograft reconstruction following resection of primary musculoskeletal tumors from 1991 to 2016. Mean patient age was 32 ± 20 years (range, 5-71 years). Minimum follow-up was 2 years unless patients were lost to disease prior. Mean follow-up was 105 months. Results: Thirty-five patients had complications requiring subsequent surgery at a mean of 30 months (range, 1--146 months) post-operatively. Individual complication rates were 29%, 50%, and 42% for Allograft Prosthetic Composite, Intercalary, and Osteoarticular allograft reconstruction, respectively. Risk factors for complication included age less than 30 (OR 4.5; p=0.002), male gender (OR 2.8; p=0.031), chemotherapy (OR 4.4; p=0.003), lower extremity disease (OR 3.4; p=0.025). In patients with complications, limb-retention rate was 91% and mean MSTS scores were 23.6. Conclusion: Despite considerable complication rates, management with a systematic approach results in successful outcomes with limb-retention greater than 90% and mean MSTS scores of 79%. In carefully selected patients, allografts provide a reliable method of reconstruction with treatable complications occurring at a mean of 30 months. [ABSTRACT FROM AUTHOR]

Introduction Hereditary haemorrhagic telangiectasia (HHT) is a genetically heterogeneous disorder caused by mutations in the genes ENG, ACVRL1, and SMAD4. Yet the genetic cause remains unknown for some families even after exhaustive exome analysis. We hypothesised that non-coding regions of the known HHT genes may harbour variants that disrupt splicing in these cases. Methods Dna from 35 individuals with clinical findings of HHT and 2 healthy controls from 13 families underwent whole genome sequencing. additionally, 87 unrelated cases suspected to have HHT were evaluated using a custom designed next-generation sequencing panel to capture the coding and non-coding regions of ENG, ACVRL1 and SMAD4. individuals from both groups had tested negative previously for a mutation in the coding region of known HHT genes. Samples were sequenced on a HiSeq2500 instrument and data were analysed to identify novel and rare variants. results eight cases had a novel non-coding ACVRL1 variant that disrupted splicing. One family had an ACVRL1intron 9:chromosome 3 translocation, the first reported case of a translocation causing HHt. the other seven cases had a variant located within a ~300 bp ctrich 'hotspot' region of ACVRL1intron 9 that disrupted splicing. Conclusions Despite the difficulty of interpreting deep intronic variants, our study highlights the importance of non-coding regions in the disease mechanism of HHT, particularly the CT-rich hotspot region of ACVRL1intron 9. the addition of this region to HHT molecular diagnostic testing algorithms will improve clinical sensitivity. [ABSTRACT FROM AUTHOR]

Increased emphasis on competency-based learning modules and widespread departure from traditional models of Halstedian apprenticeship have made surgical simulation an increasingly appealing component of medical education. Surgical simulators are available in numerous modalities, including virtual, synthetic, animal, and non-living models. The ideal surgical simulator would facilitate the acquisition and refinement of surgical skills prior to clinical application, by mimicking the size, color, texture, recoil, and environment of the operating room. Simulation training has proven helpful for advancing specific surgical skills and techniques, aiding in early and late resident learning curves. In this review, the current applications and potential benefits of incorporating simulation-based surgical training into residency curriculum are explored in depth, specifically in the context of plastic surgery. Despite the prevalence of simulation-based training models, there is a paucity of research on integration into resident programs. Current curriculums emphasize the ability to identify anatomical landmarks and procedural steps through virtual simulation. Although transfer of these skills to the operating room is promising, careful attention must be paid to mastery versus memorization. In the authors' opinions, curriculums should involve step-wise employment of diverse models in different stages of training to assess milestones. To date, the simulation of tactile experience that is reminiscent of real-time clinical scenarios remains challenging, and a sophisticated model has yet to be established. [ABSTRACT FROM AUTHOR]

Reproductive rights are an under-theorised aspect of the Women, Peace and Security (WPS) agenda, most clearly typified in United Nations Security Council resolution (UNSCR) 1325 and successive resolutions. Yet reproductive rights are central to women’s security, health and human rights. Although they feature in the 2015 Global Study on 1325, there is less reference to reproductive rights, and to abortion specifically, in the suite of United Nations Security Council (UNSC) resolutions themselves, nor in the National Action Plans (NAPs, policy documents created by individual countries to outline their implementation plans for 1325). Through content analysis of all resolutions and NAPs produced to date, this article asks where abortion is in the WPS agenda. It argues that the growing centrality of the WPS agenda to women’s rights in transitioning societies means that a lack of focus on abortion will marginalize the topic and stifle the development of liberal legalization. [ABSTRACT FROM AUTHOR]

Abstract: Inbreeding and relationship metrics among and within populations are useful measures for genetic management of wild populations, but accuracy and precision of estimates can be influenced by the number of individual genotypes analysed. Biologists are confronted with varied advice regarding the sample size necessary for reliable estimates when using genomic tools. We developed a simulation framework to identify the optimal sample size for three widely used metrics to enable quantification of expected variance and relative bias of estimates and a comparison of results among populations. We applied this approach to analyse empirical genomic data for 30 individuals from each of four different free‐ranging Rocky Mountain bighorn sheep (Ovis canadensis canadensis) populations in Montana and Wyoming, USA, through cross‐species application of an Ovine array and analysis of approximately 14,000 single nucleotide polymorphisms (SNPs) after filtering. We examined intra‐ and interpopulation relationships using kinship and identity by state metrics, as well as FST between populations. By evaluating our simulation results, we concluded that a sample size of 25 was adequate for assessing these metrics using the Ovine array to genotype Rocky Mountain bighorn sheep herds. However, we conclude that a universal sample size rule may not be able to sufficiently address the complexities that impact genomic kinship and inbreeding estimates. Thus, we recommend that a pilot study and sample size simulation using R code we developed that includes empirical genotypes from a subset of populations of interest would be an effective approach to ensure rigour in estimating genomic kinship and population differentiation. [ABSTRACT FROM AUTHOR]

Abstract: Background: Aphasia assessment is traditionally divided into formal and informal approaches. Informal assessment is useful in developing a rich understanding of the person with aphasia's performance, e.g., describing performance in the context of real‐world activities, and exploring the impact of environmental and/or partner supports upon communication. However, defining ‘informal assessment’ is problematic and can result in clinical issues including idiosyncratic practices regarding why, when and how to apply informal assessment. Aims: To examine the extent to which the informal assessment literature can guide speech and language therapists (SLTs) in their clinical application of informal assessment for post‐stroke aphasia. Methods & Procedures: A scoping review methodology was used. A systematic search of electronic databases (Scopus, Embase, PyscInfo, CINAHL, Ovid Medline and AMED) gave informal assessment references between 2000 and 2017 to which title/abstract and full‐text screening against inclusion criteria were applied. Data were extracted from 28 resulting documents using an extraction template with fields based on the review's purpose. Main Contribution: This review examines the informal assessment guidance regarding: rationale; areas of interest for informal assessment; available methods; procedural guidance; documentation; and analytical frameworks. The rationale for using informal assessment included several aspects such as gaining a ‘representative’ sample of the individual's language. Ten communication areas of interest were found with 13 different assessment methods. The procedural guidance for these methods varied considerably, with the exception of conversation and semi‐structured interviewing. Overall, documentation guidance was limited but numerous analytical frameworks were found. Conclusions: Several informal assessment methods are available to SLTs. However, information is mixed regarding when they might be used or how they might be applied in terms of their administration, documentation and analysis. [ABSTRACT FROM AUTHOR]

Feminist institutionalism is concerned with the ‘rules of the game’ in political institutions. It is interested to explore how institutions create gender-just conditions in terms of the policies and actions they undertake and the make-up of the elected representatives they contain. It also has a growing interest in how institutions can resist or obstruct positive gendered change. It is argued here that employing the concept of ‘critical actors’ alongside a feminist institutionalist framework can further our understanding of why some institutions resist change. Using the example of abortion legislation in Northern Ireland, this article illustrates how the literatures on feminist institutionalism and critical actors can, when combined, help to build a fuller narrative of why gendered policy change does not happen. [ABSTRACT FROM AUTHOR]

The year 2015 marked the 20th year of the commercialisation of genetically modified (GM) crops. During the period from 1996 to 2014, the global hectarage of these crops increased 100-fold, making it the fastest adopted crop technology in recent times. The overall economic gains from these crops have been estimated to be USD133.4 billion over the period from 1996 to 2013, and have been divided roughly 50% each to farmers in developed and developing countries. The environmental benefits include contributing to the practice of minimal till agriculture and a decrease in the use of pesticides. But what are the downsides of this technology? In this review I look at some of the problems related to weeds becoming resistant to glyphosate (the main ingredient that is used on herbicide tolerant crops), how these can be overcome and whether glyphosate can cause cancer. I also discuss the problem of insects becoming resistant to the toxins that are used in insect resistant crops and how these are being addressed. I look at what scientists around the world are saying on this topic and then consider GM crops that are in the pipeline of benefit to developing countries and whether any of these are likely to be commercialised in the foreseeable future. [ABSTRACT FROM AUTHOR]

Dairy heifers were subjected to a non-life-threatening challenge designed to induce ruminal acidosis by feeding grain and sugar. Large among animal variation in clinical signs of acidosis, rumen metabolite concentrations, and the rumen microbiome occurred. This exploratory study investigates sources of the variation by examining associations between the genome, metabolome, and microbiome, albeit with a limited population. The broader objective is to provide a rationale for a larger field study to identify markers for susceptibility to ruminal acidosis. Initially, heifers (n = 40) allocated to five feed additive groups were fed 20-days pre-challenge with a total mixed ration and additives. Fructose (0.1% of bodyweight/day) was added for the last 10 days pre-challenge. On day 21 heifers were challenged with 1.0% of bodyweight dry matter wheat + 0.2% of bodyweight fructose + additives. Rumen samples were collected via stomach tube weekly (day 0, 7, and 14) and at five times over 3.6 h after challenge and analyzed for pH and volatile fatty acid, ammonia, D-, and L-lactate concentrations. Relative abundance of bacteria and archaea were determined using Illumina MiSeq. Genotyping was undertaken using a 150K Illumina SNPchip. Genome-wide association was performed for metabolite and microbiome measures (n = 33). Few genome associations occurred with rumen pH, concentration of acetate, propionate, total volatile fatty acids, or ammonia, or the relative abundance of the Firmicutes, Bacteroidetes, and Spirochaetes phyla. Metabolites and microbial phyla that had markers associated and quantitative trait loci (QTL) were: acetate to propionate ratio (A:P), D-, L-, and total lactate, butyrate, acidosis eigenvalue, Actinobacteria, Chloroflexi, Euryarchaeota, Fibrobacteres, Planctomycetes, Proteobacteria, and Tenericutes. A putative genomic region overlapped for Actinobacteria, Euryarchaeota, and Fibrobacteres and covered the region that codes for matrix extracellular phosphoglycoprotein (MEPE). Other overlapping regions were: (1) Chloroflexi, Tenericutes, and A:P, (2) L- and total lactate and Actinobacteria, and (3) Actinobacteria, Euryarchaeota, Fibrobacteres, and A:P. Genome-wide associations with the metabolome and microbiome occurred despite the small population, suggesting that markers for ruminal acidosis susceptibility exist. The findings may explain some of the variation in metabolomic and microbial data and provide a rationale for a larger study with a population that has variation in acidosis. [ABSTRACT FROM AUTHOR]

Abstract: In recent years, several decisions have been made regarding the devolution of abortion laws from central government at Westminster to the devolved regions of the United Kingdom. This article considers the decision to devolve abortion law to Scotland. It addresses Westminster debates from the time, employing a discursive analysis to examine the arguments made for this legislative move. It argues that the debate was largely a proxy argument for the broader question of Westminster–Edinburgh relations and Scottish independence. It further argues that utilising abortion in this way is problematic, and politicises an area which is better seen as an issue solely of women's rights. [ABSTRACT FROM AUTHOR]

Feminist critics of power-sharing argue that power-sharing structures privilege ethnic/ethnonational identity and impede women's descriptive and substantive political representation. This paper extends these arguments to consider the extent to which consociational theory addresses the role of civil society and women's political voice in postconflict societies. We argue that power-sharing is overly concerned with formal representation to the detriment of understanding the role civil society can play in peace building. Whilst we acknowledge the importance of civil society retaining a critical distance from political institutions, we suggest several mechanisms for incorporating civil society into power-sharing arrangements. We argue that a consideration of civil society can highlight the gendered issues that are ignored in power-sharing settings, and we conclude that a broader understanding of both “politics” and “conflict” is required for power-sharing to be more equitable to women's descriptive and substantive representation. [ABSTRACT FROM PUBLISHER]

Background. The scapholunate interosseous ligament (SLIL) is an important contributor to wrist stability and functionality. SLIL injury is debilitating and therefore many surgical techniques have been proposed, but the optimal treatment modality remains debated. This meta-analysis reviews the available literature comparing surgical techniques used in the treatment of chronic SLIL to determine the best approach. Methods. An electronic search of the literature was conducted to identify all randomized controlled trials and cohort studies published before January 2019 that evaluated clinical outcomes of capsulodesis reconstruction, the modified Brunelli technique, and the reduction and association of the scaphoid and lunate (RASL) procedure for treatment of chronic SLIL. A chi-square analysis was performed to identify possible differences between each technique for several outcome measures. Results. A total 20 studies encompassing 409 patients met inclusion criteria. Average age among patients was 36.7 years, and 68.2% of patients were male. Reductions in visual analog scale pain scale; Disabilities of Arm, Shoulder, and Hand (DASH) scores; and increases in grip strength and range of motion were observed for all techniques. Capsulodesis was superior to the modified Brunelli technique regarding preserved range of motion. Conclusions. No significant differences were observed among any of the techniques for pain, DASH score, and grip strength outcomes. Capsulodesis, modified Brunelli, and RASL surgical techniques for the treatment of chronic SLIL injuries may all be seen as reliable methods of treatment of chronic SLIL injuries. While future trials directly comparing these methods are needed, this study suggests there is no superiority of one technique over another. [ABSTRACT FROM AUTHOR]

Genetic selection for efficiency traits is essential to improve the profitability and sustainability of livestock operations. Inefficient metabolism and energy production in animal physiology leads to excess feed consumption, resulting in higher feed costs. The objective of this study was to investigate hepatic gene expression in mature Angus-influenced cows that were classified as low or high RFI as heifers and had either a low or high ratio of calf weaning weight to mature body weight at weaning. Eleven cows were selected from the Northern Agricultural Research Center (NARC) research herd. Cows were ranked as efficient (n = 5) or inefficient (n = 6) based on their low or high residual feed intake (RFI) measured as a growing heifer and then by the ratio of calf weaning weight by mature body weight. Animals were partitioned from the bottom quartile with low RFI as a heifer and weaned with a higher proportion of the calf were classified as efficient, and animals from the top quartile with a high RFI as a heifer and a lower ratio of the calf to body weight were classified as inefficient. Liver samples were collected by needle biopsy approximately one week after weaning and snap frozen. The liver gene expression profile was measured using RNAseq. Two cows' data were not reported, one from each group, as RNA quality prevented sequencing (Efficient n = 4 and Inefficient n = 5). Over 5 million reads were mapped for each sample and were aligned to BTau 4.6.1 reference sequence using HISAT2. Differentially expressed genes were determined using the DEseq2 R package using a model based on the negative binomial distribution, and p-values were adjusted using the Benjamini- Hochberg approach. A corrected P-value of less than 0.05 defined a differentially expressed gene. Gene lists were used for gene ontology and KEGG pathway enrichment using clusterProfiler software. One novel gene was significantly differentially expressed with higher expression in the efficient group. However, no annotation currently exists for this gene. Additionally, the KEGG pathway biosynthesis of unsaturated fatty acids was significantly enriched (P < 0.05) with the following differentially expressed genes SCD, FADS1/2, ELOVL6, and HACD2, all being down-regulated in inefficient cows. The enrichment of this pathway is most functionally relevant, as it indicates that local liver changes could directly affect the efficient metabolism and biosynthesis of fatty acids. With encouraging, but limited results, this study provides the groundwork for future study of the heritable mechanisms contributing to the generation of more energy-efficient cows. [ABSTRACT FROM AUTHOR]

A number of new crops have been developed that address important traits of particular relevance for smallholder farmers in Africa. Scientists, policy makers, and other stakeholders have raised concerns that the approval process for these new crops causes delays that are often scientifically unjustified. This article develops a real option model for the optimal regulation of a risky technology that enhances economic welfare and reduces malnutrition. We consider gradual adoption of the technology and show that delaying approval reduces uncertainty about perceived risks of the technology. Optimal conditions for approval incorporate parameters of the stochastic processes governing the dynamics of risk. The model is applied to three cases of improved crops, which either are, or are expected to be, delayed by the regulatory process. The benefits and costs of the crops are presented in a partial equilibrium that considers changes in adoption over time and the foregone benefits caused by a delay in approval under irreversibility and uncertainty. We derive the equilibrium conditions where the net-benefits of the technology equal the costs that would justify a delay. The sooner information about the safety of the technology arrive, the lower the costs for justifying a delay need to be i.e. it pays more to delay. The costs of a delay can be substantial: e.g. a one year delay in approval of the pod-borer resistant cowpea in Nigeria will cost the country about 33 million USD to 46 million USD and between 100 and 3,000 lives. [ABSTRACT FROM AUTHOR]

Purpose/aim: Memory impairment post-TBI is common, frequently persistent, and functionally debilitating. The purposes of this pilot study were to assess and to compare immediate behavioral auditory working memory and electrophysiologic effects of three different, randomized, conditions of left dorsolateral prefrontal cortex (LDLPFC) transcranial direct current stimulation (tDCS) applied to four neurotypical adults and four adults with chronic traumatic brain injury (TBI).Materials/methods: Pre- and post-anodal, cathodal, and sham tDCS auditory memory performance, auditory event-related potentials (P300 amplitude and latency) and power of alpha and theta EEG bands were measured across individuals in each group.Results: Post-anodal tDCS only, the neurotypical and TBI groups both demonstrated significantly improved immediate auditory memory function. Also post-anodal tDCS, the TBI group demonstrated significantly increased P300 amplitude versus post-sham tDCS. The neurotypical group demonstrated no pre- post-tDCS electrophysiologic changes across conditions.Conclusions: These findings are consistent with findings of other studies of immediate tDCS effects on other types of memory in neurotypical individuals and in individuals with Parkinson's disease, Alzheimer's disease and stroke and suggest that individuals with memory impairments second to chronic TBI may benefit from LDLPFC anodal tDCS. Pairing tDCS with traditional behavioral memory interventions may facilitate TBI rehabilitation outcomes and warrants continued investigation. [ABSTRACT FROM PUBLISHER]