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Objective: This study assessed the status of dynamic thiol/disulfide balance (Dt/dB) among pregnant women throughout pregnancy in order to examine the possible interaction between iron deficiency anemia (IDA) and oxidative stress (OS). Methods: The investigation was designed as a prospective cohort study at the Obstetric Care Service of the Haseki Training and Research Hospital (Istanbul). Ninety pregnant women were divided into 2 groups: 45 with IDA and 45 without this condition. They were also placed into first-, second-, and third-trimester groups, with 15 participants in each group. The study gathered sociodemographic information and conducted analyses on blood samples to assess circulating (Dt/dB) status, ferritin, iron, total iron-binding capacity, and other pertinent hematological and biochemical factors. Results: There were no notable disparities observed in the thiol/disulfide equilibrium between pregnant women with or without IDA. Nevertheless, notable disparities were determined when comparing circulating disulfide values, as well as reduced thiol ratio (RTR) and thiol oxidation-reduction ratio (TORR) in relation to ferritin levels. Women with ferritin levels <20 µg/L exhibited considerably lower circulating disulfide values and significantly higher RTR and TORR values. Conclusions: Pregnant women with low levels of circulating ferritin show signs of heightened OS, as demonstrated by changes in Dt/dB measures. Tracking ferritin levels offers a more precise evaluation of OS, indicating that ferritin is a crucial indicator for OS in this specific group. It is crucial to conduct additional studies using larger and more similar groups in order to confirm these findings and enhance clinical results for both mothers and their infants. [ABSTRACT FROM AUTHOR]

Background: Vitamin B12 is an important vitamin for metabolism and affects many mechanisms in the body including neuronal migration, DNA synthesis, neurotransmitter synthesis, brain and cognitive development. Increased oxidative stress in the body leads to the damage of the child development, but also plays a crucial role in the pathogenesis of many diseases encountered in the childhood period. Our aim is to investigate whether or not B12 deficiency is associated with dynamic thiol/disulfide homeostasis in adolescent patients. Methods: This is a case-controlled observational study consisting of 45 adolescent patients with vitamin b12 deficiency and a control group consisting of 45 healthy adolescent. Patients between 11 and 18 ages who applied to the outpatient clinic for the first time with one of the complaints of headache were selected due to their decreased school performance, dizziness, and fatigue. Hemogram, vitamin B12, homocysteine levels and oxidative stress parameters such as native and total thiol disulfide levels and ratios of disulfide/native thiol, disulfide/total thiol, and native thiol/total thiol were measured from the patients. Results: Vitamin B12 level was found to be significantly lower in vitamin B12 deficiency group (p < 0.001). The serum disulfide level was found to be 27.5 ± 8.38 in the case group and 20.5 ± 8.36 in the control group (p < 0.001). In the multiple linear regression analysis, it was determined that the independent variables of native thiol, homocysteine and disulfide levels effected of vitamin B12 levels (p < 0.001, p < 0.001, p < 0.005 respectively; R2 = 0.62). Conclusion: The results obtained in terms of the effect of vitamin B12 deficiency on oxidative stress in adolescents are remarkable. The increase in oxidative stress parameters in the patient group may also suggest that oxidative stress plays a vital role in vitamin B12 deficiency in adolescence. [ABSTRACT FROM AUTHOR]

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Purpose: To evaluate thiol/disulfide homeostasis in both maternal and fetal compartment in the presence of fetal growth restriction (FGR).Materials and Methods: A prospective case-control study was carried out in women with FGR (n: 40) or normally growing fetus (n: 40). FGR was defined as estimated fetal weight below the 10th percentile for the gestational age. Maternal serum and fetal cord blood samples were collected from all participants and native thiol-disulfide exchanges were examined with automated method enabling the measurement of both sides of thiol-disulfide balance.Results: Native thiol, total thiol and disulfide amounts were decreased in the maternal serum and fetal cord blood of babies born to women with FGR (p < .05). The most sensitive marker was maternal native thiol (82.5, 95% confidence interval, 67.22-92.66%), while the maternal total thiol had highest specificity value (77.5, 95% confidence interval, 61.55-89.16%).Conclusions: Maternal and fetal serum thiol/disulfide profiles may use prediction of FGR severity and its neonatal outcome. [ABSTRACT FROM AUTHOR]