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Summary: The phytohormone abscisic acid (ABA) functions in the control of plant stress responses, particularly in drought stress. A significant mechanism in attenuating and terminating ABA signals involves regulated protein turnover, with certain ABA receptors, despite their main presence in the cytosol and nucleus, subjected to vacuolar degradation via the Endosomal Sorting Complex Required for Transport (ESCRT) machinery.Collectively our findings show that discrete TOM1‐LIKE (TOL) proteins, which are functional ESCRT‐0 complex substitutes in plants, affect the trafficking for degradation of core components of the ABA signaling and transport machinery.TOL2,3,5 and 6 modulate ABA signaling where they function additively in degradation of ubiquitinated ABA receptors and transporters.TOLs colocalize with their cargo in different endocytic compartments in the root epidermis and in guard cells of stomata, where they potentially function in ABA‐controlled stomatal aperture.Although the tol2/3/5/6 quadruple mutant plant line is significantly more drought‐tolerant and has a higher ABA sensitivity than control plant lines, it has no obvious growth or development phenotype under standard conditions, making the TOL genes ideal candidates for engineering to improved plant performance. [ABSTRACT FROM AUTHOR]

This paper aimed to discuss the areal extent and spatial structure of neighborhood, called tol, which possibly demarcate urban tissue with respect to worship of the deity and funerary acts. Bhaktapur is known as one of the historical cities of the World Heritage Site in Kathmandu Valley. The analysis firstly focuses on the dwelling distributions that have the common votary area of the guardian deities' temples/shrines and the use of the same crossing for funerary acts. Then, the authors compare those dwelling distribution with the areal extent of tols to find out how tol boundaries were formed. This result will be a precondition to conduct further examination on historical urban formation in the coming paper. [ABSTRACT FROM AUTHOR]

To assess factors determining uterine rupture during labor after the previous cesarean section among mothers delivered at Hawassa University Comprehensive Specialized Hospital from September 2017 to September 2022.Methods: A facility-based unmatched case–control study was done by reviewing 105 patients, which included 35 cases and 70 controls in a 1:2 case-to-control ratio. The association between dependent and independent variables was sought with running binary and multivariate analyses by using the cut point of a p value < 0.05 and 95% CI.Results: The prevalence of uterine rupture is 1.6%. The factors significantly associated with uterine rupture after trial of labor are fetal weight > 3.8 kg (AOR: 5.21), antenatal care 4 (AOR: 3.6), labor duration > 15 hours (AOR: 10.7), and previous successful vaginal delivery (AOR: 3.4). Poor fetal-maternal outcomes like 91.4% fetal death, 29 hysterectomies, 22 blood transfusions, and 1 death.Conclusion: The prevalence is relatively higher than in developed countries. The number of antenatal care, labor duration, and lower fetal weight are not common findings associated with uterine rupture after trial of labor across the literature, so large-scale studies are needed to develop guidelines for the Ethiopian setup. Improving the quality of obstetrics care given in each level of health system. [ABSTRACT FROM AUTHOR]

Paclitaxel (PTX) is frequently utilized for the chemotherapy of breast cancer, but its continuous treatment provokes hyposensitivity. Here, we established a PTX-resistant variant of human breast cancer MCF7 cells and found that acquiring the chemoresistance elicits a remarkable up-regulation of aldo-keto reductase (AKR) 1C3. MCF7 cell sensitivity to PTX toxicity was increased by pretreatment with AKR1C3 inhibitor and knockdown of this enzyme, and decreased by its overexpression, inferring a crucial role of AKR1C3 in the development of PTX resistance. The PTX-resistant cells were much less sensitive to 4-hydroxy-2-nonenal and acrolein, cytotoxic reactive aldehydes derived from ROS-mediated lipid peroxidation, compared with the parental cells. Additionally, the resistant cells lowered levels of 4-hydroxy-2-nonenal formed during PTX treatment, which was mitigated by pretreating with AKR1C3 inhibitor, suggesting that AKR1C3 procures the chemoresistance through facilitating the metabolism of the cytotoxic aldehyde. The gain of PTX resistance additively promoted the aberrant expression of an ATP-binding cassette (ABC) transporter ABCB1 among the ABC transporter isoforms. The combined treatment with AKR1C3 and ABCB1 inhibitors overcame the PTX resistance and cross-resistance to another taxane-based drug docetaxel. Collectively, combined treatment with AKR1C3 and ABCB1 inhibitors may exert an overcoming effect of PTX resistance in breast cancer. [ABSTRACT FROM AUTHOR]

Background: Cognitive dysfunction is a common clinical feature of mental disorders. A number of functional near-infrared spectroscopy (fNIRS) studies have shown reduced prefrontal activation during the verbal fluency task (VFT) in schizophrenia (SZ) and major depressive disorder (MDD). However, no studies have examined and compared the brain activation patterns during the Tower of London (TOL), which is another classic, high-sensitivity executive function testing tool, in these two serious mental disorders. This study aimed to assess the characteristics of brain activation during the two different cognitive tasks in SZ and MDD patients. Methods: This study recruited 30 patients with SZ, 30 patients with MDD, and 30 demographically matched healthy controls (HCs). The hemodynamic changes of the prefrontal cortex (PFC) were measured using 32-channel fNIRS during performance of the TOL task and VFT task. Results: SZ patients showed poorer VFT performance than MDD patients and HCs, and the two patient groups showed poorer TOL performance than HCs. Compared to HCs, both of the patient groups exhibited a significant decreased activation in the extensive PFC. Particularly in certain channels in the dorsolateral PFC (DLPFC), SZ patients exhibited significantly decreased hemodynamic changes than the MDD patients. Conclusions: Patients with SZ and MDD have different levels of impairment in different cognitive domains and different patterns of brain activation during the two cognitive tasks. Further research is needed to determine the use of fNIRS for clinical evaluation and diagnosis. [ABSTRACT FROM AUTHOR]

The Xinkan, Jicaquean, and Lencan language families are isolates. However, they show similarities in their vowel co-occurrence restrictions within morphemes. This paper compares and contrasts the harmonic patterns in each of these language families and concludes that similarities are likely due to contact between their speakers. This suggests that Xinkan, Jicaquean and Lencan speakers existed in a sort of micro-language area, a small-scale scenario of contact that shows unique indications of contact near or within the more dispersed scenarios of contact and borrowing acknowledged throughout Mesoamerica. [ABSTRACT FROM AUTHOR]

Defining structural features of IDPs (intrinsically disordered proteins) and relating these to biological function requires characterization of their dynamical properties. In the present paper, we review what is known about the IDPs of colicins, protein antibiotics that use their IDPs to enter bacterial cells. The structurally characterized colicin IDPs we consider contain linear binding epitopes for proteins within their target cells that the colicin hijacks during entry. We show that these binding epitopes take part in intramolecular interactions in the absence of protein partners, i.e. self-recognition, and consider the structural origins of this and its functional implications. We suggest that self-recognition is common in other IDPs that contain similar types of binding epitopes. [ABSTRACT FROM AUTHOR]

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Many Genesis interpreters view Gen. 11.27-12.9 as the foundation of the Abraham narrative (Gen. 11.27-25.11) and the opening response to the Primeval History (Gen. 1-11). Employing a narrative critical approach that is sensitive to diachronic issues, this article proposes that 11.27-12.9 also functions as a response to the preceding Tower of Babel episode (11.1-9). The Tower of Babel scene leaves readers with substantive gaps in their knowledge about the fate of the divine-human relationship. The suspense generated by such knowledge gaps is initially resolved in the introduction to the Terah-Abram narrative (11.27-12.9). This article first offers a literary rationale for reading Gen. 11.27-12.9 in light of 11.1-9, and then considers how suspense and partial resolution are expressed through verbal repetition of 'reputation', plot equivalencies, and contrasts related to migration and settlement, and a variation on the theme of capability. [ABSTRACT FROM AUTHOR]

Objective:To assess maternal postpartum and neonatal outcomes associated with previous method of delivery.Study Design:We analyzed prospectively collected maternal and neonatal data from July 2002 to December 2003. Data were collected from dedicated perinatal database and neonatal database from discharge and procedure codes. Groups were: (i) multiparous, prior vaginal delivery (VD), and (ii) multiparous, prior cesarean (CS). This group was subdivided by subsequent pregnancy trial or no trial of labor (No TOL). Results were compared with χ2-analysis; significance P<0.05.Results:There were 17 406 births. Prior CS patients without trial of labor (TOL) required more blood transfusions, intensive care unit admissions and hospital readmissions than women with a prior VD. Prior CS patients with TOL required more aminoglycosides for postpartum infection. Term neonates of (CS) mothers without a TOL were more likely to have prolonged hospitalization and require ventilatory support.Conclusions:In their subsequent delivery, women with a prior CS delivery are at significant risks for postpartum maternal and neonatal morbidities compared with parous patients who experienced a prior VD. [ABSTRACT FROM AUTHOR]

The Tol system is a five-protein assembly parasitized by colicins and bacteriophages that helps stabilize the Gram-negative outer membrane (OM). We show that allosteric signalling through the six-bladed β-propeller protein TolB is central to Tol function in Escherichia coli and that this is subverted by colicins such as ColE9 to initiate their OM translocation. Protein–protein interactions with the TolB β-propeller govern two conformational states that are adopted by the distal N-terminal 12 residues of TolB that bind TolA in the inner membrane. ColE9 promotes disorder of this ‘TolA box’ and recruitment of TolA. In contrast to ColE9, binding of the OM lipoprotein Pal to the same site induces conformational changes that sequester the TolA box to the TolB surface in which it exhibits little or no TolA binding. Our data suggest that Pal is an OFF switch for the Tol assembly, whereas colicins promote an ON state even though mimicking Pal. Comparison of the TolB mechanism to that of vertebrate guanine nucleotide exchange factor RCC1 suggests that allosteric signalling may be more prevalent in β-propeller proteins than currently realized. [ABSTRACT FROM AUTHOR]

The tol-pal genes play important roles in maintaining outer membrane integrity, transmembrane transportation, and cell division in Gram-negative bacteria. In Pseudomonas aeruginosa, an important human opportunistic pathogen, the tol-oprL genes are organized uniquely in three operons, orf1- tolQRA, tolB and oprL- orf2, and are regulated by iron availability. Similarity between TolQRA and the iron transport system ExbBD-TonB also exists in P. aeruginosa and they can replace each other imperfectly. It is of importance to investigate the regulation and functions of this membrane complex. In the present study, we characterized the promoters and expression profiles of the orf1- tolQRA operon and investigated the function of Orf1. Primer extension was carried out by using both isotope-labeled and florescence labeled primers and the expression profiles were determined using both lacZ and luxCDABE-based transcriptional fusions. Our results revealed two distinct promoters at the upstream region of tolQRA; the one located in front of orf1 was constitutive whereas the other within the orf1 coding region was iron regulated. Expression profiles indicate the tol genes were also downregulated by the quorum-sensing systems during the late stage of growth. Unlike tolQ and tolA, we were able to construct a viable orf1 knockout strain, and the mutant exhibited altered cell and colony morphology, providing first evidence that Orf1 plays a non-essential role in the Tol-OprL complex in P. aeruginosa. [ABSTRACT FROM AUTHOR]

The sequence elements determining the binding of the σ54-containing RNA polymerase (σ54-RNAP) to the Pu promoter of Pseudomonas putida have been examined. Contrary to previous results in related systems, we show that the integration host factor (IHF) binding stimulates the recruitment of the enzyme to the −12/−24 sequence motifs. Such a recruitment, which is fully independent of the activator of the system, XylR, requires the interaction of the C-terminal domain of the α subunit of RNAP with specific DNA sequences upstream of the IHF site which are reminiscent of the UP elements in σ70 promoters. Our data show that this interaction is mainly brought about by the distinct geometry of the promoter region caused by IHF binding and the ensuing DNA bending. These results support the view that binding of σ54-RNAP to a promoter is a step that can be subjected to regulation by factors (e.g. IHF) other than the sole intrinsic affinity of σ54-RNAP for the −12/−24 site. [ABSTRACT FROM AUTHOR]

A novel vertical field plate (VFP) structure with low specific on‐resistance (Ron,sp) is proposed. The VFP is inserted in the field oxide of the drift region with heavily doped N pillar parallel to the trench oxide layer (TOL), which depletes fully the drift region to decrease Ron,sp effectively and enhances the bulk field (ENBULF). The VFP optimises the bulk electric field to increase the breakdown voltage (BV). The BV of VFP is 668 V with an Ron,sp of 44.7 mΩ cm2, which is much lower than the silicon limit. [ABSTRACT FROM AUTHOR]