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Interactions within the tick microbiome involving symbionts, commensals, and tick‐borne pathogens (TBPs) play a pivotal role in disease ecology. This study explored temporal changes in the microbiome of Rhipicephalus microplus, an important cattle tick vector, focusing on its interaction with Anaplasma marginale. To overcome limitations inherent in sampling methods relying on questing ticks, which may not consistently reflect pathogen presence due to variations in exposure to infected hosts in nature, our study focused on ticks fed on chronically infected cattle. This approach ensures continuous pathogen exposure, providing a more comprehensive understanding of the nesting patterns of A. marginale in the R. microplus microbiome. Using next‐generation sequencing, microbiome dynamics were characterized over 2 years, revealing significant shifts in diversity, composition, and abundance. Anaplasma marginale exhibited varying associations, with its increased abundance correlating with reduced microbial diversity. Co‐occurrence networks demonstrated Anaplasma's evolving role, transitioning from diverse connections to keystone taxa status. An integrative approach involving in silico node removal unveils the impact of Anaplasma on network stability, highlighting its role in conferring robustness to the microbial community. This study provides insights into the intricate interplay between the tick microbiome and A. marginale, shedding light on potential avenues for controlling bovine anaplasmosis through microbiome manipulation. [ABSTRACT FROM AUTHOR]

Interactions within the tick microbiome involving symbionts, commensals, and tick‐borne pathogens (TBPs) play a pivotal role in disease ecology. This study explored temporal changes in the microbiome of Rhipicephalus microplus, an important cattle tick vector, focusing on its interaction with Anaplasma marginale. To overcome limitations inherent in sampling methods relying on questing ticks, which may not consistently reflect pathogen presence due to variations in exposure to infected hosts in nature, our study focused on ticks fed on chronically infected cattle. This approach ensures continuous pathogen exposure, providing a more comprehensive understanding of the nesting patterns of A. marginale in the R. microplus microbiome. Using next‐generation sequencing, microbiome dynamics were characterized over 2 years, revealing significant shifts in diversity, composition, and abundance. Anaplasma marginale exhibited varying associations, with its increased abundance correlating with reduced microbial diversity. Co‐occurrence networks demonstrated Anaplasma's evolving role, transitioning from diverse connections to keystone taxa status. An integrative approach involving in silico node removal unveils the impact of Anaplasma on network stability, highlighting its role in conferring robustness to the microbial community. This study provides insights into the intricate interplay between the tick microbiome and A. marginale, shedding light on potential avenues for controlling bovine anaplasmosis through microbiome manipulation. [ABSTRACT FROM AUTHOR]

Purpose of the article: The article presents the conclusions of an empirical study focused on the dynamics of changes of the Czech mobile communication market at the moment of significant change of the pricing policy in 2013. The evaluation of the dynamics of changes is based on the competitive space demands accelerator mechanism, which is a theoretically developed concept explaining specific links between the growing customer demands and company competiveness development. The study presented in this article has verified the validity of this concept. Methodology/methods: The presented results are significantly supported by ten years of the intensive research activity mapping aspects of competitive behaviour of mobile service providers in the Czech Republic. The research methodology is based on a qualitative research case study. Scientific aim: The main scientific aim is an empirical verification of the theoretically developed concept explaining the paradox of accelerated growth of customers' demand caused by the company endeavour to increase its competitiveness in the mobile communication industry. Findings: The development of the mobile communication market in the Czech Republic at the moment of principle change in the structure and form of offered subscriptions in associations with the pricing policy in the year 2013 proved the validity of the mechanism of the influence of the competitive space demands accelerator. Conclusions: The main conclusion can be summarised into three points. The first is the fact that a significant part of the increase in requirements and demands of the market is called by the activity of companies rather than the natural growth of customers' requirements. The second, extremely intensive depreciation of the potential advantage in the sense of competitive differentiation and perceived value can be explain by the mechanism of the accelerator. The third is confirmation of validity of criticism of the concept of sustainable advantage especially in the context of the created customer value. [ABSTRACT FROM AUTHOR]