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Objective: This study was to investigate the role of hsa-miR-155-3p and hsa-miR-155-5p as biomarkers and regulators of biological behavior in Systemic Sclerosis. Methods: A total of 10 SSc patients and 10 healthy controls were selected for the study. The expression levels of hsa-miR-155-3p and hsa-miR-155-5p in peripheral blood mononuclear cells of SSc patients and healthy controls were measured using RT-qPCR. The diagnostic value of these miRNAs was explored using Receiver Operating Characteristic curve analysis. Pearson or Spearman correlation analysis was performed to assess the correlation between miRNAs and clinical indicators in SSc patients. Potential target genes of hsa-miR-155-3p and hsa-miR-155-5p were predicted using miRDB, Targetscan, and miRDIP databases. GO functional annotation, KEGG pathway enrichment analysis, protein-protein interaction network construction, and selection of central genes were conducted. Results: The expression levels of hsa-miR-155-3p and hsamiR- 155-5p were significantly higher in PBMCs of SSc patients compared to healthy controls (P<0.001). The ROC curve analysis showed that hsa-miR-155-3p and hsa-miR-155-5p had a high diagnostic value for SSc (AUC=1, P<0.001). Correlation analysis revealed that hsamiR- 155-3p, hsa-miR-155-5p, and clinical indicators such as high-resolution CT, neutrophil percentage, lymphocyte percentage, and albumin to globulin ratio were correlated (P<0.05). The signaling pathways enriched with target genes of hsa-miR-155-3p and hsa-miR-155- 5p were closely associated with the occurrence and development of SSc fibrosis, immunity, and inflammation. Conclusions: hsa-miR-155-3p and hsa-miR-155-5p may be involved in regulating the occurrence and development of SSc fibrosis, immunity, and inflammation. They have the potential to serve as biomarkers for clinical diagnosis and treatment of SSc. [ABSTRACT FROM AUTHOR]

A plasma synthetic jet actuator with a high-frequency oblique slot was designed for supersonic flow control. The actuator's periodic operation characteristics were investigated using electrical parameter measurements and high-speed schlieren techniques. Experimental findings indicate that the actuator can stably operate within the frequency range of 0–2400 Hz. With increasing operating frequency, the breakdown voltage, maximum current, and discharge energy decrease, while the energy efficiency of the discharge increases. Moreover, the experiment revealed for the first time that an increase in the number of charged particles within the cavity leads to a decrease in breakdown voltage, and the concentration of charged particles reaches saturation at approximately 1200 Hz. High-speed schlieren observations indicate substantial inter-cycle influences in the periodic jet. At higher operating frequencies, the impact of inter-cycle flow field effects becomes more pronounced. The oblique slot plasma synthetic jet exhibits clear directionality in the distribution of shock waves and jet. The oblique slot jet also shows a significant wall attachment effect. These characteristics are beneficial for enhancing the flow control capability of the actuator. At 1200 Hz, the actuator achieves a discharge efficiency of 63% and a maximum jet front velocity of 309 m/s, showcasing its superior overall performance. [ABSTRACT FROM AUTHOR]

In the field of flow control research, oblique jets are known to offer several advantages over vertical jets. To gain a comprehensive insight into the flow field characteristics of a plasma synthetic jet actuator with an oblique-slot exit, the related experiments are conducted. The experiment employed high-speed schlieren imaging techniques and electrical parameter measurements to acquire the flow field characteristics and discharge properties of the oblique-slot actuator, followed by a comparative analysis with a vertical circular orifice actuator. The oblique-slot plasma synthetic jet exhibits a wall-attaching effect and asymmetric flow characteristics, which differ from those of the vertical circular orifice actuator. The actuator generates a wall jet with an initial velocity of 389.5 ± 15.08 m/s, effectively propelling the fluid within the boundary layer. The Mach number of the precursor shock wave in the direction of the jet reaches 1.59, but decreases to just 1.02 in the opposite direction. Over a period in the range of 10–70 μs, the Froude number of the plasma jet decreases from 1841 to 238. The dominant role of the inertial force gradually weakens, while the influence of buoyancy increases, causing the jet boundary to move upward. The oblique-slot jet configuration represents a typical planar jet, exhibiting superior flow control uniformity compared with the vertical circular orifice jet. The results indicate that the high-speed oblique-slot plasma synthetic jet actuator designed in this study possesses distinct advantages over vertical circular orifice actuators for high-speed fluid flow control. [ABSTRACT FROM AUTHOR]

Background:Cryptosporidium spp. are a type of protozoan parasite responsible for causing diarrheal illness worldwide. They infect a broad range of vertebrate hosts, including both non-human primates (NHPs) and humans. In fact, zoonotic transmission of cryptosporidiosis from NHPs to humans is frequently facilitated by direct contact between the two groups. However, there is a need to enhance the information available on the subtyping of Cryptosporidium spp. in NHPs in the Yunnan province of China. Materials and Methods: Thus, the study investigated the molecular prevalence and species of Cryptosporidium spp. from 392 stool samples of Macaca fascicularis (n = 335) and Macaca mulatta (n = 57) by using nested PCR targeting the large subunit of nuclear ribosomal RNA (LSU) gene. Of the 392 samples, 42 (10.71%) were tested Cryptosporidium-positive. Results: All the samples were identified as Cryptosporidium hominis. Further, the statistical analysis revealed that age is a risk factor for the infection of C. hominis. The probability of detecting C. hominis was found to be higher (odds ratio = 6.23, 95% confidence interval 1.73–22.38) in NHPs aged between 2 and 3 years, as compared with those younger than 2 years. Sequence analysis of the 60 kDa glycoprotein (gp60) identified six (IbA9 n = 4, IiA17 n = 5, InA23 n = 1, InA24 n = 2, InA25 n = 3, and InA26 n = 18) C. hominis subtypes with "TCA" repeats. Among these subtypes, it has been previously reported that the Ib family subtypes are also capable of infecting humans. Conclusion: The findings of this study highlight the genetic diversity of C. hominis infection among M. fascicularis and M. mulatta in Yunnan province. Further, the results confirm that both these NHPs are susceptible to C. hominis infection, posing a potential threat to humans. [ABSTRACT FROM AUTHOR]

NRP1 is a transmembrane glycoprotein that is highly expressed in a variety of tumors. There is evidence that NRP1 can enhance the stem cell properties of tumor cells, which are thought to be resistant to radiotherapy. This study aims to elucidate the potential mechanism of NRP1 in radiation resistance. We transfected NRP1 siRNA and plasmid in breast cancer cells to detect the expression of cancer stem cell markers by western blot and qRT-PCR. The effect of NRP1 on radiotherapy resistance was assesses by immunofluorescence and flow cytometry. In vivo, we established xenograft tumor model treating with shRNA-NRP1 to assess radiotherapy sensitivity. We found that NRP1 could enhance the stem cell properties and confer radioresistance of breast cancer cells. Mechanistically, we proved that NRP1 reduced IR-induced apoptosis by downregulation of Bcl-2 via methyltransferase WTAP in m6A-depentent way. It is suggested that these molecules may be the therapeutic targets for improving the efficacy of radiotherapy for breast cancer. [ABSTRACT FROM AUTHOR]

Curved ducts are widely used in aircraft engines to improve some capability of aero-engines. Complex internal flow characteristics would be induced by the curvature in such components. In this study, the influence of parameters, including the arc angle α , the curvature radius R i , and the height H, on the local accelerating and transonic flow in the curved ducts with equal area were studied numerically and theoretically under different nozzle pressure ratios (NPRs). The range of the Re number based on the height of the duct and the velocity at the inlet was 0.8 × 10 6 ∼ 1.6 × 10 6 . The shear stress transport κ-ω turbulent model was proved by the test data to suitably simulate the flow field in curved ducts because it could accurately predict the flow separations under adverse pressure gradients. The uncertainty of the pressure scan value to obtain the test data was ±0.05%. Numerical results showed that the effect of α on the flow characteristics of the curved ducts is little. The maximum Ma number in the curved section reduces with the increase of R i , and that grows with the increase of H. The range of the maximum Ma number was 1.20∼1.80. The critical NPRs, which decided the special flow features, were found in the curved ducts. The critical NPR rises with the increase of R i ; however, the effect of H on the critical NPR is irregular due to the flow separations located near the lower wall induced by the large adverse pressure gradient. The theoretical results based on the small perturbation theory of transonic flow in the polar coordinate system proved that the distribution of sonic line was just dependent on the inner diameter R 1, the outer diameter R 2, and the arc angle θ max of the curved section. The critical mass flow and the critical NPR2 are only related to R 1 and R 2. [ABSTRACT FROM AUTHOR]

The components with large curvature features are widely applied in aero-engines. Complex flow features are induced due to large curvature under high-subsonic even transonic incoming flow condition. In this study, the formation mechanisms of local acceleration in bent duct are investigated. To this end, the cold fluid test of a 60° bent duct with constant flow section was conducted. The surface static pressures and the schlieren flow visualizations were obtained. Then the three-dimensional numerical simulations based on the experimental model were computed using computational fluid dynamics software. The simulations were conducted using five different turbulence models to compare with the experimental data. The validation study shows that the shear stress transfer (SST) κ-ω turbulence model is suitably used for the simulations. Results show that three different flow situations were shown for the bent duct at diverse nozzle pressure ratios (NPRs). One situation was shown by the case at NPR = 1.5, in which the whole flow field is subsonic, and just two jet edges are shown by the schlieren images. One situation was shown by the case at NPR = 1.8, in which a local supersonic region is induced near the lower wall at the hind side of the bent section, and a small shock wave is observed. The other one situation was shown by the cases at NPR = 2.0, 2.5 and 3.0, in which the air flow in the whole passage reaches supersonic speeds and an oblique shock wave is shown for each case. [ABSTRACT FROM AUTHOR]

Background: The vital signs of trauma patients are complex and changeable, and the prediction of blood transfusion demand mainly depends on doctors' experience and trauma scoring system; therefore, it cannot be accurately predicted. In this study, a machine learning decision tree algorithm [classification and regression tree (CRT) and eXtreme gradient boosting (XGBoost)] was proposed for the demand prediction of traumatic blood transfusion to provide technical support for doctors. Methods: A total of 1371 trauma patients who were diverted to the Emergency Department of the First Medical Center of Chinese PLA General Hospital from January 2014 to January 2018 were collected from an emergency trauma database. The vital signs, laboratory examination parameters and blood transfusion volume were used as variables, and the non-invasive parameters and all (non-invasive + invasive) parameters were used to construct an intelligent prediction model for red blood cell (RBC) demand by logistic regression (LR), CRT and XGBoost. The prediction accuracy of the model was compared with the area under the curve (AUC). Results: For non-invasive parameters, the LR method was the best, with an AUC of 0.72 [95% confidence interval (CI) 0.657–0.775], which was higher than the CRT (AUC 0.69, 95% CI 0.633–0.751) and the XGBoost (AUC 0.71, 95% CI 0.654–0.756, P < 0.05). The trauma location and shock index are important prediction parameters. For all the prediction parameters, XGBoost was the best, with an AUC of 0.94 (95% CI 0.893–0.981), which was higher than the LR (AUC 0.80, 95% CI 0.744–0.850) and the CRT (AUC 0.82, 95% CI 0.779–0.853, P < 0.05). Haematocrit (Hct) is an important prediction parameter. Conclusions: The classification performance of the intelligent prediction model of red blood cell transfusion in trauma patients constructed by the decision tree algorithm is not inferior to that of the traditional LR method. It can be used as a technical support to assist doctors to make rapid and accurate blood transfusion decisions in emergency rescue environment, so as to improve the success rate of patient treatment. [ABSTRACT FROM AUTHOR]

It is commonly accepted that an estimated soil variogram can be transferred to another similar area for deriving the tolerable spacing of a sampling grid or, more generally, the sample size, given a requirement on the quality of the soil property map of the recipient area. The quality of the derived tolerable grid spacing depends on how similar the population variograms of the donor area and recipient area are. In practice we are uncertain about the variograms of both areas due to sampling errors. Ideally, the uncertainty about the variogram of the donor area is accounted for in deriving the tolerable grid spacing. To assess the transferability, we should also account for uncertainty in the estimated variogram of the recipient area. In this study the transferability of variograms of soil pH, P, Mg and K is analysed for three grassland fields in Ireland, which are similar in soil‐forming factors. One field served as donor area, the other two as recipient area. For all three fields and for each soil property, 500 variograms were sampled from the posterior distribution of the variogram parameters. Results showed that the estimated variogram parameters of the recipient fields differed largely from those of the transferred variograms. The ranges of estimated mean kriging variance values for the various grid spacings, as obtained with the two sets of variograms (one set of the donor field, one set of the recipient field), did not overlap. Even after scaling the transferred variogram with an estimate of the variance of the recipient field, the transferred variogram was of no use for determining the tolerable grid spacing. The difference in the variograms can possibly be explained by the difference in historical land use. Highlights: Transferability of variograms to derive tolerable grid spacing for mapping grassland fields is assessedTransferability should be based on the uncertainty distributions of the tolerable grid spacingsDue to difference in historical land use, local and transferred variograms differed largelyTransferability of a variogram is very poor, even after scaling the transferred variogram [ABSTRACT FROM AUTHOR]

In the 1980s, recombinant human erythropoietin (rhEPO) began to be used in clinical practice. In this study, the clinical application of rhEPO from single-center in recent ten years was reviewed, and the scope of indications and clinical efficacy were evaluated. The medical records of 35829 in-patients who were treated with rhEPO in the first Medical Center of the Chinese PLA General Hospital from 2009 to 2018 were collected. According to the scope of indications approved by CFDA (China Food and Drug Administration), curative effect and off-label of rhEPO were analyzed. Of the 35829 patients, 19013 (53.1%) were male and 16816 (46.9%) were female, with an average age of (52.1 ± 18.6) years. The usage of rhEPO is increasing year by year. The overall effective rate was 53.1%. The number of patients who met the indications accounted for 67.2%, and the effective rate patients with indications and Off-label were 48.8% and 50.7%. Among the patients with irregular use of rhEPO perioperative imperfect laboratory examination patients accounted for the highest proportion (7.1%). The volume of RBC(s) (red blood cell(s)) transfusion in patients with rhEPO was significantly less than that in patients without rhEPO (p<0.05). The use of rhEPO Off-label is very common and has a certain curative effect. It can be used as evidence support for the update of the scope of indications. In addition, There are still irregular use of rhEPO and transfusion in clinic. The unreasonable use of rhEPO and transfusion should be further standardized to ensure the safety and effectiveness. [ABSTRACT FROM AUTHOR]

Eimeria spp. and Blastocystis are the common parasites that parasitize the intestinal tract of rabbits, which can seriously threaten the health of rabbits and lead to economic losses to the rabbit industry. However, information about the prevalence and transmission of these two parasites in rabbits is limited in China. The objective of this study was to survey the prevalence of Eimeria spp. and Blastocystis in rabbits in Shandong Province. A total of 616 rabbit fecal samples were collected from two cities (Rizhao and Weihai) in Shandong Province, eastern China, and Eimeria spp. and Blastocystis were identified by polymerase chain reaction based on species-specific markers. The prevalence of Eimeria spp. was 20% (123/616) and the Blastocystis prevalence was 0.97% (6/616). Five different Eimeria species (Eimeria intestinalis, E. perforans, E. magna, E. media, and E. irresidua) and the ST4 subtype of Blastocystis were identified in rabbits by sequence analysis. This is the first report of Blastocystis prevalence and subtype ST4 in rabbits in Shandong Province. The findings provide baseline data for the prevention and control of Eimeria spp. and Blastocystis in rabbits in Shandong Province, China. [ABSTRACT FROM AUTHOR]

Axonal development is essential to the establishment of neuronal morphology and circuitry, although the mechanisms underlying axonal outgrowth during the early developmental stages remain unclear. Here, we showed that the conserved disco-interacting protein B (DIP2B) which consists of a DMAP1 domain and a crotonobetaine/carnitine CoA ligase (Caic) domain, is highly expressed in the excitatory neurons of the hippocampus. DIP2B knockout led to excessive axonal outgrowth but not polarity at an early developmental stage. Furthermore, the loss of DIP2B inhibited synaptic transmission for both spontaneous and rapid release in cultured hippocampal neurons. Interestingly, DIP2B function during axonal outgrowth requires tubulin acetylation. These findings reveal a new conserved regulator of neuronal morphology and provide a novel intervention mechanism for neurocognitive disorders. [ABSTRACT FROM AUTHOR]

Giardia duodenalis is a zoonotic enteric parasite that can infect humans and a number of animal species including rabbits with a worldwide distribution. Infection with G. duodenalis can cause serious public health problems and significant economic losses to animal husbandry. So accurate understanding of the prevalence and genotype distribution of G. duodenalis in rabbits is necessary. In the present study, a total of 616 fecal samples were collected from rabbits in Shandong province, eastern China, and examined in G. duodenalis prevalence and genotypes by nested PCR amplification of β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) gene loci of G. duodenalis. Sixty-nine (11.2%) of the examined rabbit fecal samples were G. duodenalis-positive. Of them, the prevalence of G. duodenalis is 8.4% (41/490) in Rizhao city and 22.2% (28/126) in Weihai city. Breeds, region, and feeding modes were highly correlated with G. duodenalis infection in rabbits. Moreover, three genotypes (assemblages A, B, and E) were identified in rabbits at three gene loci, and the assemblage E was the dominant genotype, while the assemblage A was reported in rabbits in China for the first time. It is noticeable that two rabbits were found to be infected with two different G. duodenalis assemblages (assemblages A and E, assemblages B and E, respectively). These findings enrich the genotype distribution of G. duodenalis in rabbits and provide baseline data for preventing and controlling G. duodenalis infection in rabbits in eastern China. [ABSTRACT FROM AUTHOR]

The effects of nitrogen on the characteristics and evolutions of M2C eutectic carbides in the Fe-Mo-W-Co-Cr-V-C alloy were investigated in both as-cast and wrought states. Microstructural observation, electrolytic extraction method, and x-ray diffraction analysis were conducted on the specimens. The results showed that, in the case of low nitrogen concentration (w[N]% = 0.006) in the as-cast alloy, lamellar M2C carbides were found to be the dominant precipitate. Nitrogen addition resulted in M2C carbide precipitate as a fibrous structure rather than a lamellar structure. Fibrous M2C were more likely to decompose into fine spherical M6C and V(C,N) during the forging process compared to lamellar M2C. Accordingly, it was suggested that adding nitrogen to Fe-Mo-W-Co-Cr-V-C alloy was required to achieve small dimensions and homogeneous distribution of carbides. [ABSTRACT FROM AUTHOR]

In digital soil mapping (DSM) there is an increasing interest in incorporating multiscale variations of soil and environmental covariates to improve mapping accuracy. Empirical mode decomposition (EMD) is a frequently used method for multiscale analysis but has been rarely applied to DSM. This study examines if DSM with EMD covariates results in a more accurate map of a test area than DSM with original covariates. First, EMD was used to decompose terrain attributes of an area. These EMD components were then used to map soil organic carbon (SOC) concentration, pH, clay content and cation exchange capacity (CEC) by two DSM methods: multiple linear regression (MLR) and regression kriging (RK). The same two DSM methods were used with original covariates; in addition, ordinary kriging (OK) maps were produced without covariates. The resulting maps were compared by various accuracy measures. The use of EMD components for DSM improved correlations between soil properties and terrain attributes, and improved the goodness‐of‐fit of MLR models significantly and prediction accuracies by 2.5–18% of root mean square error (RMSE) for SOC, clay and CEC. By contrast, the use of EMD components for pH increased the goodness‐of‐fit of the MLR model slightly and decreased the prediction accuracy by 30% of RMSE. This study concluded that the use of EMD components for DSM improved mapping accuracies if the goodness‐of‐fit of the MLR models was significantly improved by EMD components. It is possible that sampling based on EMD components may provide a sample set that would improve mapping accuracy further. Highlights: Empirical mode decomposition (EMD) components of terrain attributes were applied to soil mapping.EMD components improved correlations between soil properties and terrain attributes.EMD components improved the goodness‐of‐fit of multiple linear regression (MLR).Significant improved goodness‐of‐fit of MLR resulted in improved mapping accuracies. [ABSTRACT FROM AUTHOR]

The aim of this study was to characterize the primary carbides formed in as-cast H13 steel. The composition, morphology, type, and size of primary carbides in as-cast H13 steel were investigated by optical microscope (OM), field emission scanning electron microscopy (FE-SEM), electron back-scattered diffraction (EBSD), and X-ray diffraction (XRD) analysis. The number of primary carbides was investigated by ASPEX automated inclusion analysis system. The results indicated that primary carbides in as-cast H13 steel are mainly composed of Cr, Mo, V, and Ti, and there exist four kinds of primary carbides in the interdendritic zones of H13 steel, which are stripy Mo–Cr-rich M2C, eutectic Mo–Cr-rich M2C, V-rich MC, and V-rich MC with Ti and N. Thermodynamic calculation indicated that M2C precipitates in liquid phase at solid fractions larger than 0.99, while MC precipitates in liquid phase at solid fractions larger than 0.96. Statistical results indicated that the number of M2C is much greater than the number of other kinds of primary carbides. Most primary carbides are blocky, with lengths of no more than 10 μm and a length/width ratio of no more than 3. The large primary carbides in as-cast H13 steel are mainly M2C. [ABSTRACT FROM AUTHOR]

AISI M42 high-speed steel is prone to fracture as a result of its brittle martensitic microstructure together with abundant carbides located at the grain boundaries. In this study, a series of property tests including hardness, impact toughness, and wear loss were performed to study the effect of tempering conditions on the mechanical properties of AISI M42 high-speed steel over holding time ranging from 1 to 20 hours. The effects of the tempering time on the characteristics and growth of carbides were also investigated. The results indicated that carbides in the experimental steels were obviously coarsened when the tempering time exceeded 4 hours. The dimension of the carbides increased, while the volume fraction decreased with the increasing tempering time, and the grain sizes were significantly augmented due to the reducing of small carbides. Moreover, the dislocation density decreased with the increasing tempering time, which led to the reducing of the yield stress of high-speed steel. An appropriate holding time (4 hours) resulted in fine-scale secondary carbides and a smaller grain size, which efficiently improved the impact toughness and wear resistance simultaneously. Nevertheless, a prolonged tempering time (> 4 hours) promoted the coarsening and coalescence of carbides, which were detrimental to the impact toughness and wear resistance. Consequently, the formation of fine-scale secondary carbides is the major influential factor to improve both the wear resistance and impact toughness. [ABSTRACT FROM AUTHOR]

Background Uncontrolled blood pressure ( BP) is commonly observed in patients receiving antihypertensive agents. However, its relationship with early left ventricular ( LV) dysfunction has not been elucidated. Methods This study enrolled 276 patients with treated hypertension and 85 healthy controls. The 140/90 mm Hg was used to define controlled ( HT1 group, n=145) or uncontrolled BP ( HT2 group, n=131) according to the concurrent guidelines. LV myocardial function was assessed by two-dimensional speckle tracking imaging, and the circumferential end-systolic wall stress ( cESS)-corrected mid-wall fraction shortening ( MWFS), systolic longitudinal (εLs-18), circumferential (εCs-18), and radial (εRs-18) strain were measured. Results Despite similar ejection fraction, the HT1 and HT2 groups displayed an overall reduction in the cESS-corrected MWFS (13.4±2.7 vs 11.7±1.7 vs 15.5±1.2), εLs-18 (15.6±2.8 vs 13.0±2.2 vs 17.4±2.8), εCs-18 (17.3±3.4 vs 14.1±2.7 vs 18.9±3.3), and εRs-18 (18.4±4.0 vs 14.8±3.1 vs 20.5±4.5) %·cm2/kdyne·10−2 when compared with the control group (all P<.001). The changes were more obvious in the HT2 group, regardless of LV hypertrophy. Reductions in the cESS-corrected MWFS and εLs-18 were seen in 68 (25%) and 52 (19%) patients, respectively. Uncontrolled BP were 4.365 times (95% CI 2.203-8.648, P<.001) and 3.928 times (1.851-8.337, P<.001) more likely to be associated with the changes. Conclusions Uncontrolled BP in hypertensive patients is associated with further reduction in LV myocardial function detected by advanced echocardiographic techniques, which cannot be explained by the increase in afterload. It might be regarded as a composite risk factor for earlier and faster development of clinical heart failure, therefore, a simplified treatment target. [ABSTRACT FROM AUTHOR]

Infrared radiation signatures of gas turbine engine exhaust are suppressed markedly when equipped with a serpentine nozzle compared to an axisymmetric nozzle. The aim of this paper is to research more detailed flow characteristics of the serpentine nozzle, and to this end a double serpentine nozzle cold fluid test was conducted in this paper, static pressures on the nozzle walls surface were measured, and schlieren flow visualizations downstream of the nozzle exit were observed. Then numerical simulations of the experimental model were carried out using CFD software with k-ɛ turbulence model adopted. And the effects of geometric design parameters (the length ratio of first S length to second S length and the centerline distributions) on serpentine nozzle performance were investigated numerically. Detailed flow characteristics were presented including the distributions of static pressure, Ma number (streamlines), wall shear stress (limited streamlines), and the total pressure. Results show good agreement between the experimental data and computation. Static pressure distributions on the upper and down walls surface of double serpentine nozzle are completely different compared to the traditional axisymmetric nozzle. The rapid turning and steep passage slope of the serpentine nozzle would result in high friction loss and strong secondary flow loss, hence the value of the length ratio of first S passage to second S passage is recommended to be chosen from 2:5 to 2:3. The centerline distributions are crucial to the nozzle design for its influence on air acceleration inside the nozzle. The centerlines with a rapid turning at the exit would result in a high Ma number, which brings on high friction loss and secondary loss at the turnings. For maximum efficiency of centerline distributions, it is recommended that curves with a gentle turning at each serpentine passage exit should be chosen. [ABSTRACT FROM AUTHOR]

Information on the spatial distribution of soil particle-size fractions (psf) is required for a wide range of applications. Geostatistics is often used to map spatial distribution from point observations; however, for compositional data such as soil psf, conventional multivariate geostatistics are not optimal. Several solutions have been proposed, including compositional kriging and transformation to a composition followed by cokriging. These have been shown to perform differently in different situations, so that there is no procedure to choose an optimal method. To address this, two case studies of soil psf mapping were carried out using compositional kriging, log-ratio cokriging, cokriging, and additive log-ratio cokriging; and the performance of Mahalanobis distance as a criterion for choosing an optimal mapping method was tested. All methods generated very similar results. However, the compositional kriging and cokriging results were slightly more similar to each other than to the other pair, as were log-ratio cokriging and additive log-ratio cokriging. The similar results of the two methods within each pair were due to similarities of the methods themselves, for example, the same variogram models and prediction techniques, and the similar results between the two pairs were due to the mathematical relationship between original and log-ratio transformed data. Mahalanobis distance did not prove to be a good indicator for selecting an optimal method to map soil psf. [ABSTRACT FROM AUTHOR]

The effect of calcium treatment and/or aluminum-based deoxidant addition on the oxygen control and modification of MgO·AlO spinel inclusions during protective gas electroslag remelting (P-ESR) of H13 die steel with low oxygen content was experimentally studied. It is found that all the inclusions in the consumable electrode are MgO·AlO spinels, besides a few MgO·AlO spinels surrounded by an outer (Ti,V)N or MnS layer. After P-ESR refining combined with proper calcium treatment, all the original MgO·AlO spinels in the electrode (except for the original MgO·AlO spinels having been removed in the P-ESR process) were modified to mainly CaO-MgO-AlO and some CaO-AlO inclusions, both of which have a low melting point and homogeneous compositions. In the case of only Al-based deoxidant addition, all the oxide inclusions remaining in ESR ingots are MgO·AlO spinels. The operation of Al-based deoxidant addition and/or calcium treatment during P-ESR of electrode steel containing low oxygen content is invalid to further reduce the oxygen content and oxide inclusions amount compared with remelting only under protective gas atmosphere. All the original sulfide inclusions were removed after the P-ESR process. Most of the inclusions in ESR ingots are about 2 μm in size. The mechanisms of non-metallic inclusions evolution and modification of MgO·AlO spinels by calcium treatment during the P-ESR process were proposed. [ABSTRACT FROM AUTHOR]

Over-expression of chloroplast glycerol-3-phosphate acyltransferase gene ( LeGPAT) in tomato increased cis-unsaturated fatty acid content in phosphatidylglycerol (PG) of the thylakoid membrane. Under chilling stress, the oxygen evolving activity, the maximal photochemical efficiency of PSII ( F/ F), and superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities decreased less in sense lines than in antisense lines compared to wild-type (WT) plants. Consistently, the relative electric conductivity, $${\text{O}}_{2} ^{{. - }} $$ and HO contents in sense lines were lower than those of WT and antisense lines. The antisense lines with low level of unsaturated fatty acids in PG were extremely susceptible to photoinhibition of PSII and had a significant reduction in the D1 protein content of PSII reaction center under chilling stress. However, in the presence of streptomycin (SM), the degradation of D1 protein was faster in sense lines than in WT and antisense plants. These results suggested that, under chilling stress conditions, increasing cis-unsaturated fatty acids in PG through over-expression of LeGPAT can alleviate PSII photoinhibition by accelerating the repair of D1 protein and improving the activities of antioxidant enzymes in chloroplasts. [ABSTRACT FROM AUTHOR]

The aim of this study was to evaluate the consequence of changing land use from agriculture land to other use purposes with respect to OCPs non-cancer and cancer risk on human health, based on concentrations of DDTs and HCHs in soils collected from 55 locations representing 12 different land use types. There were no non-cancer risks of DDTs and γ-HCHs on adults and children, and there were very low cancer risks of DDD, o,p′-DDE, DDT, α-HCH, β-HCH, γ-HCH based on their total concentrations in all samples. Nonetheless, there were significant correlations of DDT to its metabolites (DDE and DDD) (r= 0.506 and r= 0.648) and DDE to DDD (r= 0.438) both at p< 0.01. OCP levels should be routinely monitored in different environmental media and food in order to verify whether there is fresh input. Their potential risks on human health should also be assessed. [ABSTRACT FROM AUTHOR]

Toxoplasma gondii is an obligate intracellular protozoan of severe threat to humans and livestock, whose life history harbors both gamic and apogamic stages. Chinese 1 (ToxoDB#9) was a preponderant genotype epidemic in food-derived animals and humans in China, with a different pathogenesis from the strains from the other nations of the world. Posttranslational modifications (PTMs) of proteins were critical mediators of the biology, developmental transforms, and pathogenesis of protozoan parasites. The phosphoprotein profiling and the difference between the developmental phases of T. gondii, contributing to development and infectivity, remain unknown. A quantitative phosphoproteomic approach using IBT integrated with TiO2 affinity chromatography was applied to identify and analyze the difference in the phosphoproteomes between the sporulated oocysts and the tachyzoites of the virulent ToxoDB#9 (PYS) strain of T. gondii. A total of 4058 differential phosphopeptides, consisting of 2597 upregulated and 1461 downregulated phosphopeptides, were characterized between sporulated the oocysts and tachyzoites. Twenty-one motifs extracted from the upregulated phosphopeptides contained 19 serine motifs and 2 threonine motifs (GxxTP and TP), whereas 16 motifs identified from downregulated phosphopeptides included 13 serine motifs and 3 threonine motifs (KxxT, RxxT, and TP). Beyond the traditional kinases, some infrequent classes of kinases, including Ab1, EGFR, INSR, Jak, Src and Syk, were found to be corresponding to motifs from the upregulated and downregulated phosphopeptides. Remarkable functional properties of the differentially expressed phosphoproteins were discovered by GO analysis, KEGG pathway analysis, and STRING analysis. S8GFS8 (DNMT1-RFD domain-containing protein) and S8F5G5 (Histone kinase SNF1) were the two most connected peptides in the kinase-associated network. Out of these, phosphorylated modifications in histone kinase SNF1 have functioned in mitosis and interphase of T. gondii, as well as in the regulation of gene expression relevant to differentiation. Our study discovered a remarkable difference in the abundance of phosphopeptides between the sporulated oocysts and tachyzoites of the virulent ToxoDB#9 (PYS) strain of T. gondii, which may provide a new resource for understanding stage-specific differences in PTMs and may enhance the illustration of the regulatory mechanisms contributing to the development and infectivity of T. gondii. [ABSTRACT FROM AUTHOR]

Background: Community health service center (CHSC) in China is always regarded as a good facility of primary care, which plays an important role in chronic non-communicable disease management. This study aimed to investigate the blood pressure (BP) control rate in a real life CHSC-based management program and its determinants.Methods: The study enrolled 3191 patients (mean age of 70 ± 10 years, 43% males) in a hypertension management program provided by the Yulin CHSC (Chengdu, China), which had been running for 9 years. Uncontrolled BP was defined as the systolic BP of ≥140 mmHg and/or the diastolic BP of ≥90 mmHg, and its associated factors were analyzed by using logistic regression.Results: The duration of stay in the program was 33 ± 25 months. When compared with the BP at entry, the recent BP was significantly lowered (147 ± 17 vs. 133 ± 8 mmHg; 83 ± 11 vs. 75 ± 6 mmHg) and the BP control rate was dramatically increased (32 vs. 85%) (all p < 0.001). The age of >70 years [1.40 (odds ratio), 1.15-1.71 (95% confidence interval)], female gender (0.76, 0.63-0.93), longer stay of >33 months (0.77, 0.63-0.94), doctor in charge (0.97, 0.95-0.99), and the use of calcium channel blocker (1.35, 1.09-1.67) were significantly related to uncontrolled BP at the recent follow up (all p < 0.05).Conclusions: This CHSC-run hypertension program provides an ideal platform of multi-intervention management, which is effective in achieving higher BP control rate in community patient population. However, the BP control status could be affected by age, gender and adherence of the patients, as well as practice behavior of the doctors. [ABSTRACT FROM AUTHOR]