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The Embodiment Lab, rooted in critical human geography, is grounded in embodiment, belonging, mentorship, care, and temporal dynamics to challenge norms in the neoliberal university. We argue that the Lab serves as a counter-practice within the academy by prioritizing our individual and collective well-being over productivity metrics. Weekly practices cultivate radical vulnerability, creating a foundation for a caring environment. Delving into multifaceted spatial dimensions our experiences suggest that the Lab becomes a living example of a feminist ethic of care. Belonging emerges as an antidote to the exclusions ingrained in academic spaces. The Lab empowers its scholars to challenge uneven power dynamics, fostering inclusion where diverse voices are heard. The Lab's emphasis on collective action and intentional processes of growth contrasts with a conventional fast, metric-driven tempo. In this paper, we offer a model to center care in lab spaces by reflecting on our own experiences in a space that values scholars as whole individuals rather than vessels of productivity. We illustrate the reflexive character of the Lab, acknowledging its adaptability and dynamism over time. Rejecting the neoliberal norms that too often dictate research spaces, the Lab exemplifies the messy and ongoing process of creating care-full academic spaces. [ABSTRACT FROM AUTHOR]

Tissue engineering is an interdisciplinary field that combines materials, methods, and biological molecules to engineer newly formed tissues to replace or restore functional organs. Biomaterials-based scaffolds play a crucial role in developing new tissue by interacting with human cells. Tissue engineering scaffolds with ideal characteristics, namely, nontoxicity, biodegradability, and appropriate mechanical and surface properties, are vital for tissue regeneration applications. However, current biocomposite scaffolds face significant limitations, particularly in achieving structural durability, controlled degradation rates, and effective cellular integration. These qualities are essential for maintaining long-term functionality in vivo. Although commonly utilized biomaterials can provide physical and chemical properties needed for tissue regeneration, inadequate biomimetic properties, as well as insufficient interactions of cells-scaffolds interaction, still need to be improved for the application of tissue engineering in vivo. It is impossible to achieve some essential features using a single material, so combining two or more materials may accomplish the requirements. In order to achieve a proper scaffold design, a suitable fabrication technique and combination of biomaterials with controlled micro or nanostructures are needed to achieve the proper biological responses. This review emphasizes advancements in scaffold durability, biocompatibility, and cellular responsiveness. It focuses on natural and synthetic polymer combinations and innovative fabrication techniques. Developing stimulus-responsive 3D scaffolds is critical, as these scaffolds enhance cell adhesion and promote functional tissue formation while maintaining structural integrity over time. This review also highlights the natural polymers, smart materials, and recent advanced techniques currently used to create emerging scaffolds for tissue regeneration applications. [ABSTRACT FROM AUTHOR]

Pectin has recently drawn much attention in biomedical applications due to its distinctive chemical and biological properties. Polymers like pectin with cell-instructive properties are attractive natural biomaterials for tissue repair and regeneration. In addition, bioactive pectin and pectin-based composites exhibit improved characteristics to deliver active molecules. Pectin and pectin-based composites serve as interactive matrices or scaffolds by stimulating cell adhesion and cell proliferation and enhancing tissue remodeling by forming an extracellular matrix in vivo. Several bioactive properties, such as immunoregulatory, antibacterial, anti-inflammatory, anti-tumor, and antioxidant activities, contribute to the pectin's and pectin-based composite's enhanced applications in tissue engineering and drug delivery systems. Tissue engineering scaffolds containing pectin and pectin-based conjugates or composites demonstrate essential features such as nontoxicity, tunable mechanical properties, biodegradability, and suitable surface properties. The design and fabrication of pectic composites are versatile for tissue engineering and drug delivery applications. This article reviews the promising characteristics of pectin or pectic polysaccharides and pectin-based composites and highlights their potential biomedical applications, focusing on drug delivery and tissue engineering. [ABSTRACT FROM AUTHOR]

The international development community has approached SDG 12 (Responsible Consumption and Production) through the lens of specific supply chains of consumer goods and services. For example, minerals from mines to markets; wood from forests to furniture; or food from farm to fridge, have been tracked in terms of their ecological profile in many of the SDG 12 targets. While such an approach can give us some idea of particular recycling or refurbishment opportunities, as well as waste-toenergy generation, it lacks a systems-oriented view on the interlinkages between socio-ecological systems of consumption and production. We argue that SDG 12 needs to be reimagined in terms of lateral impacts and connections in key sectors of resource extraction. Sand mining and saltwater intrusion (SWI) present an important example of how such a connection could be made between an anthropogenic activity in a coastal / marine environment and its ecological impact that could threaten food security. We present a review of research in this context that links these two seemingly disparate areas of academic inquiry. Focusing on the Mekong Delta we also consider how geospatial techniques could help to evaluate these connected impacts between sand mining and SWI and its consequential impacts on arable land and hence food availability and hunger. Considering a series of methodological challenges, we offer a way forward for measuring these impacts and charting a more integrative way forward for operationalizing SDG12 towards more sustainable environmental and social outcomes. [ABSTRACT FROM AUTHOR]

Conventional control of sucking pests infesting various vegetables has several drawbacks. Biorational insecticides are increasingly used for pest management due to their limited side effects. A study involving several biorational compounds, viz. spinosad, buprofezin, Beauveria bassiana, Lecanicillium muscarium and a chemical insecticide, fenitrothion was performed by leaf-dip bioassay to measure their efficacy against the bean aphid, A. craccivora Koch. as a representative of sucking pests. Mortality of aphids against treatment with biorational insecticides was recorded in three replicates under laboratory conditions. The lethal concentration (LC50 and LC90) of the treatments were calculated by probit analysis. Efficacy was concentration and time dependent. Spinosad has been found to be more effective than buprofezin. The spinosad (LC50 = 2582.04 ppm) and buprofezin (LC50 = 543.03 ppm) were found to be approximately 88 and 52 times less toxic than fenitrothion. At 48 h post-treatment (HPT), the highest mortality for spinosad and buprofezin was 66.67 and 60.00%, respectively, and reached 93.33% at 72 HPT. Among the two fungal biopesticides, L. muscarium was more effective than B. bassiana. For both, mortality (80–100%) at the higher concentrations was statistically different than others at 144 and 168 HPT. Thus, biorational compounds, particularly spinosad and L. muscarium, are useful for controlling bean aphids. [ABSTRACT FROM AUTHOR]

Well-characterized and scalable downstream processes for the purification of biologics are extremely demanding for delivering quality therapeutics to patients at a reasonable price. Erythropoietin (EPO) is a blockbuster biologic with diverse clinical applications, but its application is limited to financially well-off societies due to its high price. The high price of EPO is associated with the technical difficulties related to the purification challenge to obtain qualified products with a cost-effective defined process. Though there are reports for the purification of EPO there is no report of a well-characterized downstream process with critical process parameters (CPPs) that can deliver EPO consistently satisfying the quality target product profile (QTPP), which is a critical regulatory requirement. To advance the field, we applied the quality by design (QbD) principle and design of experiment (DoE) protocol to establish an effective process, which is scalable up to 100× batch size satisfying QTPP. We have successfully transformed the process from static mode to dynamic mode and validated it. Insignificant variation (p > 0.05) within and between 1×, 10×, and 100× batches showed that the process is reproducible and seamlessly scalable. The biochemical analysis along with the biofunctionality data ensures that the products from different scale batches were indifferent and comparable to a reference product. Our study thereby established a robust and scalable downstream process of EPO biosimilar satisfying QTPP. The technological scheme presented here can speed up the production of not only EPO but also many other life-saving biologics and make them available to the mass population at a reduced cost. [ABSTRACT FROM AUTHOR]

Slaughterhouse and wet market wastes are pollutants that have been always neglected by society. According to the Food and Agriculture Organization of the United Nations, more than three billion and nineteen million livestock were consumed worldwide in 2018, which reflects the vast amount and the broad spectrum of the biowastes generated. Slaughterhouse biowastes are a significant volume of biohazards that poses a high risk of contamination to the environment, an outbreak of diseases, and insecure food safety. This work comprehensively reviewed existing biowaste disposal practices and revealed the limitations of technological advancements to eradicate the threat of possible harmful infectious agents from these wastes. Policies, including strict supervision and uniform minimum hygienic regulations at all raw food processing factories, should therefore be tightened to ensure the protection of the food supply. The vast quantity of biowastes also offers a zero-waste potential for a circular economy, but the incorporation of biowaste recycling, including composting, anaerobic digestion, and thermal treatment, nevertheless remains challenging. [ABSTRACT FROM AUTHOR]

A field experiment was conducted at Bangladesh Agricultural University farm, Mymensingh, Bangladesh, during the Boro season, 2017 with six different K fertilizer rates: 0, 10, 20, 30, 40 and 50 kg ha-1 to determine the optimum rate of potassium (K) fertilization for the improved yield of a specific rice variety in the irrigated ecosystem under floodplain area. Compared with no K fertilizer, adding K increased the rice grain and straw yields significantly, with all other yield contributing components, except 1000-grain weight. The highest yield of grain (7.07 ton ha-1) and straw (8.48 ton ha-1) were recorded in recommended fertilizer dose (RFD) of NPS + 50kg K treatment, which were statistically identical with RFD of NPS + 40 kg K. Rice grain and straw yields due to the different treatments increased by 18.65% to 53.74% and 18.67% to 53.78%, respectively over control. K content and uptake through grain and straw were significantly influenced by applying different levels of K. These results specified that the use of 40 kg K ha-1 had better performance on the grain and straw yields. Therefore, we conclude that the application of 40 kg K ha-1 along with the RFD of NPS for BRRI dhan29 cultivation is the best option for higher yield in Old Brahmaputra Floodplain soil. [ABSTRACT FROM AUTHOR]

A refractive index (RI) sensor has been demonstrated and numerically analyzed by the finite element method (FEM) to gain supreme sensitivity on the basis of localized surface plasmon resonance (LSPR). Plasmonic material, such as silver, has been used as a coating layer outside of the dielectric core. Various structural parameters have been analyzed for the proposed sensors, which have a significant influence on the sensing field. To make the sensing strength much stronger, a thin MgF2 layer has been added to the silver layer. The wavelength and amplitude interrogation method achieves extremely high wavelength sensitivity (WS) responses of 948.67 μm/RIU and amplitude sensitivity (AS) responses of − 1602.82 RIU−1 in the analyte RI range of 1.35 to 1.38. The operating wavelength range of 5.5–7.5 μm shows efficient results in this structure. The proposed sensor is used for identifying analytes in the biological and biochemical fields and is also applicable in bio-photonics. Owing to its low confinement losses and simply designed structure, the proposed RI sensor will be extensively appropriate for bio-sensing because of its outstanding sensitivity response. [ABSTRACT FROM AUTHOR]

Background: The COVID-19 remains a public health burden that has caused global economic crises, jeopardizing health, jobs, and livelihoods of millions of people around the globe. Several efforts have been made by several countries by implementing several health strategies to attenuate the spread of the pandemic. Although several studies indicated effects of COVID-19 on mental health and its associated factors, very little is known about the underlying mechanism of job insecurity, depression, anxiety, and stress in Bangladesh. Therefore, this study determined the prevalence of job insecurity and depression, anxiety, stress as well as the association between job insecurity, mental health outcomes also contributing determinants amongst humanitarian workers during the COVID-19 pandemic in Bangladesh. Methods: We conducted a web-based cross-sectional study among 445 humanitarian workers during the COVID-19 pandemic in six sub-districts of Cox's bazar district of Bangladesh between April and May 2021. The questionnaire was composed of socio-demographic, lifestyle and work related factors. Psychometric instruments like job insecurity scale and depression, anxiety also stress scale (DASS-21) were employed to assess the level of job insecurity and mental health outcomes (depression, anxiety and stress). STATA software version 14 was employed to perform statistical analyses. Results: The prevalence of job insecurity was 42%. The odds of job insecurity was higher in Kutubdia and Pekua (AOR = 3.1, 95% CI 1.36, 7.22) Teknaf (AOR = 2.9, 95% CI 1.33, 6.41), the impact of dissatisfaction on salary (AOR = 2.3, 95% CI 1.49, 3.58) was evident with job insecurity. The prevalence of moderate to severe depression, anxiety and stress among humanitarian worker were (26%, 7%), (25%, 10%) and (15%, 7%) respectively. Further, the region of work, being female, marital status, work environment, and salary dissatisfaction were contributing factors for poor mental health outcomes. Those with job insecurity were almost 3 times more likely to experience depression (AOR = 2.7, 95% CI 1.85, 4.04), anxiety (AOR = 2.6, 95% CI 1.76, 3.71) and stress (AOR: 2.8; 95% CI 1.89, 4.26), respectively. Conclusion: Our findings highlight that job security remains essential to help tackle the severity of depression, anxiety and stress in humanitarian workers. The results reflected the critical importance of local and international NGOs addressing poor mental health conditions of their employees to prevent mental health outbreaks. [ABSTRACT FROM AUTHOR]

Lipid nanoparticle (LNP) technology has become extremely demanding for delivering RNA-products and other drugs. However, there is no platform to manufacture pharmaceutical-grade LNPs with desired particle size from a wide range in continuous mode. We have developed a unique platform to obtain any specific size-range of LNPs from 60 to 180 nm satisfying pharmaceutical regulatory requirements for polydispersity index, sterility, dose uniformity and bio-functionality. We applied design of experiment (DoE) methodology and identified the critical process parameters to establish the process for global application. Cross-point validation within the response map of DoE confirmed that the platform is robust to produce specific size (± 10 nm) of LNPs within the design-range. The technology is successfully transformed to production scale and validated. Products from R&D, pilot and production batches for a candidate SARS-CoV-2 mRNA-vaccine generated equivalent biological responses. The data collectively established the robustness and bio-uniformity of doses for global RNA-vaccine/drug formulation. [ABSTRACT FROM AUTHOR]

This study investigated groundwater pollution and potential human health risks from arsenic, iron, and manganese in the rural area of Jashore, Bangladesh. Study results show that the mean value of groundwater pH is 7.25+0.31, with a mean conductivity of 633.94+ 327.41 µs/cm, while about 73, 97, and 91% of groundwater samples exceeded the Bangladesh drinking water standard limits for As, Fe, and Mn, respectively. Groundwater pollution evaluation indices, including the heavy metal pollution index, the heavy metal evaluation index, the degree of contamination, and the Nemerow pollution index, show that approximately 97, 82, 100, and 100% of samples are in the high degree of pollution category, respectively. Spatial distribution exhibited that the study area is highly exposed to As (73%), Fe (82%), and Mn (46%). In the case of non-carcinogenic health risk via oral exposure, about 94% of samples suggest a high category of risk for infants, and 97% of samples are found to be at high risk for children and adults. The carcinogenic risk of arsenic via an oral exposure pathway suggests that approximately 97% of the samples are found to be at high risk for infants, and all of the samples are at high risk for both adults and children. [ABSTRACT FROM AUTHOR]

Aim: Poor farmers are cultivating tobacco because they can obtain great profit and support from tobacco companies. However, they overlook tobacco-related health problems and consequences. In this study, we explored the impact of tobacco farming on farmers' health, drawing on an empirical study in seven villages of Ruposhipara union, Bandarban district in Bangladesh. Subjects and methods: Following a mixed research method, data were collected by administering 200 pre-tested structured questionnaires, five group discussions and five in-depth interviews. We also reviewed existing and proposed policies related to tobacco control in Bangladesh. We followed grounded theory for analysing qualitative data and conducted a Chi-square test to observe any significant associations for selected quantitative data. Results: Farmers' self-reported tobacco-related sicknesses were nausea, vomiting, dizziness, headache, weakness, cough, dyspnoea and hypersalivation. Children, women and older people were equally affected. They experienced diverse tobacco-related health problems at different stages of tobacco farming. Continuous contact with tobacco leaves and not using any safety measures had put the farmers at greater risk of tobacco sickness. Conclusion: The proposed "Tobacco Cultivation Control Policy, 2017" emphasised to provide necessary supports to farmers for growing alternative crops. Implementation of this policy would encourage farmers to give up harmful tobacco farming and lessen their health problems. [ABSTRACT FROM AUTHOR]

The objective of this research is to understand the psychological and livelihood-related impacts of coronavirus disease 2019 (COVID-19) on Bangladeshi lower income group people who depend on daily earnings for their living. Following the convenience sampling method, 576 respondents were interviewed for quantitative data and 30 in-depth interviews for qualitative information in several districts of Bangladesh. To 94.1% respondents, livelihood has been affected by the COVID-19 outbreak with an overall score of 3.20 ± 0.77 on a 4-point Likert-type scale. In comparison to unemployed respondents, daily workers have been hardly affected by the COVID-19 outbreak (odds ratio [OR] = 7.957; P <.01), and so they are going outside more frequently in search of jobs (OR = 9.984, P <.01). Due to fear of COVID-19 infection and lack of livelihood means, respondents (76.6%) have been stressed out (overall score 3.19 ± 0.81 on a 4-point Likert-type scale), and those working in industries (OR = 5.818, P <.01), farmers (OR = 3.029, P <.05), and day laborers (OR = 2.651, P <.05) have been highly stressed. [ABSTRACT FROM AUTHOR]

Non-thermal atmospheric pressure plasma jet (NTAPPJ) was shown as a promising tool for sterilization, treatment of chronic wounds & tumors. A portable and facile NTAPPJ system will broaden the application of plasma for many other biomedical applications. This paper demonstrates the feasibility of a customized dimmer controllable atmospheric pressure plasma jet (APPJ) in treating HeLa cells. A plasma torch was energized at 8 kVpp and 35 kHz from a neon transformer and discharged with argon gas-producing plasma plume of various gas species. The output intensity of the plasma discharge was adjusted by the voltage input from the dimmer controller circuit. Between 60 - 220 V, a different power output of the dimmer can be varied between 8.4 to 83.6 W, to generate different intensities of non-thermal APPJ (NTAPPJ). The NTAPPJ was then applied to HeLa cells for 20 sec & it was found that cells reduced their adhesion at a dimmer power < 20 W but stayed viable. This progressed to observations of damaged cell body when the power was increased beyond 20 W & the cell membranes shattered when the power was up to 83.6 W. This was due to the ultraviolet rays and reactive oxygen species (ROS), that induced detrimental effects to the cells. The controllable NTAPPJ system, based on dimmer control, induced different levels of effects on the biophysical structures of cells, ranging from reduced cell spread to apoptosis and eventually cell membrane disintegration. [ABSTRACT FROM AUTHOR]

Non-thermal atmospheric plasma jet (NTAPPJ) is believed to be effective in treating monolayer cells cultured in-vitro due to the oxidative stresses induced. However, it is controversial whether studying the impact of plasma to cells in monolayer can be representative to a model close to the in-vivo tissues. This study revealed and compared the effects of NTAPPJ treatment directly to both monolayer of HeLa cells and microspheroids in the culture media, at different exposure time from 15 to 60 seconds at a fixed power of 80 W. Optical emission spectroscopy (OES) revealed that the plasma applied to the cells is rich in hydroxyl, nitrogen, oxygen and argon species. The experimental results revealed that the treatment time from 15 seconds onwards was detrimental to the monolayer of cells, but not to the spheroids compared with the negative controls. After long NTAPPJ exposure, the HeLa cells in the spheroids were found viable, regardless of the plasma exposure time applied. The multicellular structure of the spheroids has prevented the penetration of free radicals into the cells encapsulated in the spheroids. [ABSTRACT FROM AUTHOR]

Growing three dimensional (3D) cells is an emerging research in tissue engineering. Biophysical properties of the 3D cells regulate the cells growth, drug diffusion dynamics and gene expressions. Scaffold based or scaffoldless techniques for 3D cell cultures are rarely being compared in terms of the physical features of the microtissues produced. The biophysical properties of the microtissues cultured using scaffold based microencapsulation by flicking and scaffoldless liquid crystal (LC) based techniques were characterized. Flicking technique produced high yield and highly reproducible microtissues of keratinocyte cell lines in alginate microcapsules at approximately 350 ± 12 pieces per culture. However, microtissues grown on the LC substrates yielded at lower quantity of 58 ± 21 pieces per culture. The sizes of the microtissues produced using alginate microcapsules and LC substrates were 250 ± 25 µm and 141 ± 70 µm, respectively. In both techniques, cells remodeled into microtissues via different growth phases and showed good integrity of cells in field-emission scanning microscopy (FE-SEM). Microencapsulation packed the cells in alginate scaffolds of polysaccharides with limited spaces for motility. Whereas, LC substrates allowed the cells to migrate and self-stacking into multilayered structures as revealed by the nuclei stainings. The cells cultured using both techniques were found viable based on the live and dead cell stainings. Stained histological sections showed that both techniques produced cell models that closely replicate the intrinsic physiological conditions. Alginate microcapsulation and LC based techniques produced microtissues containing similar bio-macromolecules but they did not alter the main absorption bands of microtissues as revealed by the Fourier transform infrared spectroscopy. Cell growth, structural organization, morphology and surface structures for 3D microtissues cultured using both techniques appeared to be different and might be suitable for different applications. [ABSTRACT FROM AUTHOR]

Considering the important factor of bioactive nanohydoxyapatite (nHA) to enhance osteoconductivity or bone-bonding capacity, nHA was incorporated into an electrospun polycaprolactone (PCL) membrane using electrospinning techniques. The viscosity of the PCL and nHA/PCL with different concentrations of nHA was measured and the morphology of the electrospun membranes was compared using a field emission scanning electron microscopy. The water contact angle of the nanofiber determined the wettability of the membranes of different concentrations. The surface roughness of the electrospun nanofibers fabricated from pure PCL and nHA/PCL was determined and compared using atomic force microscopy. Attenuated total reflectance Fourier transform infrared spectroscopy was used to study the chemical bonding of the composite electrospun nanofibers. Beadless nanofibers were achieved after the incorporation of nHA with a diameter of 200-700 nm. Results showed that the fiber diameter and the surface roughness of electrospun nanofibers were significantly increased after the incorporation of nHA. In contrast, the water contact angle (132° ± 3.5°) was reduced for PCL membrane after addition of 10% (w/w) nHA (112° ± 3.0°). Ultimate tensile strengths of PCL membrane and 10% (w/w) nHA/PCL membrane were 25.02 ± 2.3 and 18.5 ± 4.4 MPa. A model drug tetracycline hydrochloride was successfully loaded in the membrane and the membrane demonstrated good antibacterial effects against the growth of bacteria by showing inhibition zone for E. coli (2.53 ± 0.06 cm) and B. cereus (2.87 ± 0.06 cm). [ABSTRACT FROM AUTHOR]

Considering the important factor of bioactive nanohydoxyapatite (nHA) to enhance osteoconductivity or bone-bonding capacity, nHA was incorporated into an electrospun polycaprolactone (PCL) membrane using electrospinning techniques. The viscosity of the PCL and nHA/PCL with different concentrations of nHA was measured and the morphology of the electrospun membranes was compared using a field emission scanning electron microscopy. The water contact angle of the nanofiber determined the wettability of the membranes of different concentrations. The surface roughness of the electrospun nanofibers fabricated from pure PCL and nHA/PCL was determined and compared using atomic force microscopy. Attenuated total reflectance Fourier transform infrared spectroscopy was used to study the chemical bonding of the composite electrospun nanofibers. Beadless nanofibers were achieved after the incorporation of nHA with a diameter of 200-700 nm. Results showed that the fiber diameter and the surface roughness of electrospun nanofibers were significantly increased after the incorporation of nHA. In contrast, the water contact angle (132° ± 3.5°) was reduced for PCL membrane after addition of 10% (w/w) nHA (112° ± 3.0°). Ultimate tensile strengths of PCL membrane and 10% (w/w) nHA/PCL membrane were 25.02 ± 2.3 and 18.5 ± 4.4 MPa. A model drug tetracycline hydrochloride was successfully loaded in the membrane and the membrane demonstrated good antibacterial effects against the growth of bacteria by showing inhibition zone for E. coli (2.53 ± 0.06 cm) and B. cereus (2.87 ± 0.06 cm). [ABSTRACT FROM AUTHOR]

The development of nano-sized scaffolds with antibacterial properties that mimic the architecture of tissue is one of the challenges in tissue engineering. In this study, polycaprolactone (PCL) and PCL/gelatine (Ge) (70:30) nanofibrous scaffolds were fabricated using a less toxic and common solvent, formic acid and an electrospinning technique. Nanofibrous scaffolds were coated with silver (Ag) in different concentrations of silver nitrate (AgNO) aqueous solution (1.25, 2.5, 5, and 10 %) by using dipping method, drying and followed by ultraviolet (UV) photoreduction. The PCL/Ge (70:30) nanofibrous scaffold had an average fibre diameter of 155.60 ± 41.13 nm. Characterization showed that Ag was physically entrapped in both the PCL and PCL/Ge (70:30) nanofibrous scaffolds. Ag ions release study was performed and showed much lesser release amount than the maximum toxic concentration of Ag ions in human cells. Both scaffolds were non-toxic to cells and demonstrated antibacterial effects towards Gram-positive Bacillus cereus ( B. cereus) and Gram-negative Escherichia coli ( E. coli). The Ag/PCL/Ge (70:30) nanofibrous scaffold has potential for tissue engineering as it can protect wounds from bacterial infection and promote tissue regeneration. [ABSTRACT FROM AUTHOR]

Background: Despite high prevalence of intimate partner violence (IPV) and its adverse social and health consequences, the rate of help seeking for IPV is generally low. Although the level of IPV is much higher in urban slums of Bangladesh, the level and nature of help seeking of the victims are unknown. This paper aims to address this gap in the literature.Methods: Using a cross-sectional survey conducted between August 2011-February 2012, we explored disclosure of violence, help seeking behavior, and their correlates among randomly selected currently married women aged 15-29 in Dhaka slums (n = 2604).Results: About 60 % of the currently married women reported past year spousal physical violence, but only 21 % disclosed and 19 % sought any help. High acceptance of violence was the main reason for not seeking help. Help was most commonly sought from informal sources (89 %). Any education, frequent and severe physical abuse, and presence of children increased the likelihood of disclosure and help seeking. Most survivors from slum who disclosed also sought help.Conclusions: Despite widespread physical abuse, many survivors never sought help. Wide acceptance of violence hampering help seeking needs to be challenged. Increasing disclosure would also enhance help seeking. Awareness rising regarding rights of women to live a violence free life is essential. Although many services are available in the urban area, information about these services needs to be available to women. Promoting education is important in increasing both disclosure and service uptake. [ABSTRACT FROM AUTHOR]

Cellulose-based materials are one of the most commonly used materials for biomedical applications, which normally applied as carriers for pharmaceuticals and drug-releasing scaffolds. In this study, cellulose acetate (CA) was used to fabricate the nanofibrous membrane using the electrospinning technique. CA solutions at different concentrations were prepared by dissolving the polymer in a mixture of acetic acid/acetone solvents with the ratio of 3 : 1. The field emission scanning electron microscope results showed that electrospinning of 10% (w/v) CA produced nanofibres with many beads. When the CA concentration was increased to 14% (w/v), bead-free nanofibres were produced. The contact angle measurement results confirmed the hydrophilic properties of nanofibres. In order to prevent common bacterial infections, a model drug, Tetracycline ⋅ HCL was incorporated into the CA nanofibres. The drug-loaded CA nanofibres showed antibacterial activity against Gram-positive and Gram-negative bacteria. CA nanofibres had high water uptake properties. The CA nanofibrous membrane was non-toxic to human skin fibroblast cells. Thus the CA nanofibres with 14% (w/v) concentration exerted suitable properties for wound healing application. [ABSTRACT FROM AUTHOR]

Investigation of the serum level of copper, zinc, magnesium and manganese in type-2 diabetes mellitus and their possible association with lipid profile was carried out. The comparative study included 100 type-2 diabetic patients in Gr-II and 100 non-diabetic as control in (Gr-I). Results indicated that there is a significant lower level (p < .001) of serum Zn, Cu, Mg and Mn in diabetic patients compared with the control group, showing p value < .001. In type-2 DM patients (Gr-II) there were significant correlations between serum Zn and TAG (r = 0.209) and between Zn and HDL-C level (r = .199) showing p value <.05. Non significant relationships were found in between Zn and lipid profile (TAG, Cholesterol, HDL-C and LDL-C) of control (Gr-I) group. Significant correlation was found between serum magnesium and TAG of control (Gr-I) where p < .01 and non significant correlations were found in serum Mg and total cholesterol, HDL-C, LDL-C of both type-2 diabetic (Gr-II) and control (Gr-I). Significant correlations were also found in between serum of Cu and Mn and TAG of control (Gr-I) where p < .05 and non significant correlations were found in other component of lipid profile of both cases (Gr-II) and control (Gr-I). [ABSTRACT FROM AUTHOR]

This article reports the fabrication of three-dimensional porous chitosan and hydroxyapatite (HA)/chitosan composite scaffolds by the thermally induced phase separation (TIPS) technique, for bone tissue engineering. Different amounts of HA nanoparticles (10%, 20%, and 30% g/g) were added to the chitosan solution to produce HA/chitosan composite scaffolds of varying compositions. The morphology and pore structure of the scaffolds vis-à-vis composition were characterized using scanning electron microscopy (SEM) and an energy dispersive X-ray (EDX). Both pure chitosan and HA/chitosan composite scaffolds were highly porous and had interconnected pores. The pore sizes ranged from several micrometers to a few hundred micrometers. The HA nanoparticles were well dispersed and physically coexisted with chitosan in the composite scaffolds. However, some agglomeration of HA nanoparticles was observed on the surface of pore walls when a relatively large amount of HA was used. The composite 3D scaffolds are very promising for use in bone tissue engineering application. [ABSTRACT FROM PUBLISHER]

Biodegradable polymer-based scaffolds containing osteoconductive hydroxyapatite (HA) particles can be very useful for bone tissue engineering. In this investigation, HA nanoparticles were incorporated in poly(hydroxybutyrate- co-valerate) (PHBV) polymer to fabricate osteoconductive composite scaffolds. PHBV and HA/PHBV scaffolds were made using an emulsion freezing/freeze-drying technique. The scaffolds produced were subsequently characterized using several techniques. It was found that the scaffolds were highly porous and had interconnected porous structures. The pore size ranged from several microns to around 300 μm. The spherical HA nanoparticles which were produced in-house through a nanoemulsion process could be incorporated into composite scaffolds although some of these nanoparticles existed on the surface of pore walls when a relatively large amount of HA was used for composite scaffolds. The incorporation of HA nanoparticles also enhanced compressive mechanical properties of the scaffolds. [ABSTRACT FROM AUTHOR]

Heart development is a precisely coordinated process of cellular proliferation, migration, differentiation, and integrated morphogenetic interactions, and therefore it is highly susceptible to developmental anomalies such as the congenital heart disease (CHD). One of the major causes of CHD has been shown to be the mutations in key cardiac transcription factors, including nkx2.5. Here, we report the analysis of zebrafish mutant ftk that showed a progressive heart malformation in the later stages of heart morphogenesis. Our analyses revealed that the cardiac muscle maturation and heart morphogenesis in ftk mutants were impaired because of the disorganization of myofibrils. Notably, we found that the expression of nkx2.5 was down-regulated in the ftk heart despite the normal expression of gata4 and tbx5. suggesting a common mechanism for the occurrence of ftk phenotype and CHD. We identified ftk to be a loss-of-function mutation in a connexin gene, cx36.7/early cardiac connexin (ecx), expressed during early heart development. We further showed by a rescue experiment that Nkx2.5 is the downstream mediator of Ecx-mediated signaling. From these results, we propose that the cardiac connexin Ecx and its downstream signaling are crucial for establishing nkx2.5 expression, which in turn promotes unidirectional, parallel alignment of myofibrils and the subsequent proper heart morphogenesis. [ABSTRACT FROM AUTHOR]

Background: Next-generation sequencing (NGS) has been advancing the progress of detection of disease-associated genetic variants and genome-wide profiling of expressed sequences over the past decade. NGS enables the analyses of multiple regions of a genome in a single reaction format and has been shown to be a cost-effective and efficient tool for root-cause analysis of disease and optimization of treatment. NGS has been leading global efforts to device personalized and precision medicine (PM) in clinical practice. The effectiveness of NGS for the aforementioned applications has been proven unequivocal for multifactorial diseases like cancer. However, definitive prediction of cancer markers for all types of diseases and for global populations still remains highly rewarding because of the diversity of cancer types and genetic variants in human. Results: We performed exome sequencing of four samples in quest of critical genetic factor/s associated with liver cancer. By imposing knowledge-based filter chains, we have revealed a panel of genetic variants, which are unrecognized by current major genomics data repositories. Total 20 MNV-induced, 5 INDEL-induced, and 31 SNV-induced neoplasm-exclusive genes were revealed through NGS data acquisition followed by data curing with the application of quality filter chains. Liver-specific expression profile of the identified gene pool is directed to the selection of 17 genes which could be the as likely causative genetic factors for liver cancer. Further study on expression level and relevant functional significance enables us to identify and conclude the following four novel variants, viz., c.416T>C (p.Phe139Ser) in SORD, c.1048_1049delGCinsCG (p.Ala350Arg) in KRT6A, c.1159G>T (p.Gly387Cys) in SVEP1, and c.430G>C (p.Gly144Arg) in MRPL38 as a critical genetic factor for liver cancer. Conclusion: By applying a novel data prioritizing rationale, we explored a panel of previously unaddressed liver cancer-associated variants. These findings may have an opportunity for early prediction of neoplasm/cancer in liver and designing of relevant personalized/precision liver cancer therapeutics in clinical practice. Since NGS protocol is associated with tons of non-specific mutations due to the variation in background genetic makeup of subjects, therefore, our method of data curing could be applicable for more effective screening of global genetic variants related to disease onset, progression, and remission. [ABSTRACT FROM AUTHOR]

In this study, biodegradable and biocompatible polylactic acid (PLA) and poly(3-hyroxybutyrate-co-3-hydroxyvalerate) (PHBV) blend polymer solutions were electrospun using electrospinning technique. Polymer blend ratio and electrospinning voltage were varied and optimized. The fabricated electrospun PLA/PHBV membranes were dipped into poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) solution to produce conductive PEDOT:PSS coated membranes. The coated and uncoated membranes were investigated using scanning electron microscopy (SEM), field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) and water uptake measurement. PEDOT:PSS coated membranes were then loaded with drug (tetracycline hydrochloride) and their antibacterial activity was investigated. Beadless PLA/PHBV membranes were successfully fabricated. The fabricated PLA/PHBV membranes were rendered conductive by dipping them into poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) solution. Drug loaded membranes showed antibacterial properties which was confirmed by zone inhibition method. [ABSTRACT FROM AUTHOR]