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The performance of version 4 of the NOAA High-Resolution Rapid Refresh (HRRR) numerical weather prediction model for near-surface variables, including wind, humidity, temperature, surface latent and sensible fluxes, and longwave and shortwave radiative fluxes, is examined over the Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP) region. The study evaluated the model’s bias and bias-corrected mean absolute error relative to the observations on different time scales. Forecasts of near-surface geophysical variables at five SGP sites (HRRR at 3-km scale) were found to agree well with observations, but some consistent observation–forecast differences also occurred. Sensible and latent heat fluxes are the most challenging variables to be reproduced. The diurnal cycle is the main temporal scale affecting observation–forecast differences of the near-surface variables, and almost all of the variables showed different biases throughout the diurnal cycle. Results show that the overestimation of downward shortwave and the underestimation of downward longwave radiative flux are the two major biases found in this study. The timing and magnitude of downward longwave flux, wind speed, and sensible and latent heat fluxes are also different with contributions from model representations, data assimilation limitations, and differences in scales between HRRR and SGP sites. The positive bias in downward shortwave and negative bias in longwave radiation suggests that the model is underestimating cloud fraction in the study domain. The study concludes by showing a brief comparison with version 3 of the HRRR and shows that version 4 has better performance in almost all near-surface variables. [ABSTRACT FROM AUTHOR]

Application of lake models coupled within earth-system prediction models, especially for predictions from days to weeks, requires accurate initialization of lake temperatures. Commonly used methods to initialize lake temperatures include interpolation of global sea-surface temperature (SST) analyses to inland lakes, daily satellite-based observations, or model-based reanalyses. However, each of these methods have limitations in capturing the temporal characteristics of lake temperatures (e.g., effects of anomalously warm or cold weather) for all lakes within a geographic region and/or during extended cloudy periods. An alternative lake-initialization method was developed which uses two-way-coupled cycling of a small-lake model within an hourly data assimilation system of a weather prediction model. The lake model simulated lake temperatures were compared with other estimates from satellite and in situ observations and interpolated-SST data for a multi-month period in 2021. The lake cycling initialization, now applied to two operational US NOAA weather models, was found to decrease errors in lake surface temperature from as much as 5–10 K vs. interpolated-SST data to about 1–2 K compared to available in situ and satellite observations. [ABSTRACT FROM AUTHOR]

A technique for model initialization using three-dimensional radar reflectivity data has been developed and applied within the NOAA 13-km Rapid Refresh (RAP) and 3-km High-Resolution Rapid Refresh (HRRR) regional forecast systems. This technique enabled the first assimilation of radar reflectivity data for operational NOAA forecast models, critical especially for more accurate short-range prediction of convective storms. For the RAP, the technique uses a diabatic digital filter initialization (DFI) procedure originally deployed to control initial inertial gravity wave noise. Within the forward-model integration portion of diabatic DFI, temperature tendencies obtained from the model cloud/precipitation processes are replaced by specified latent heating-based temperature tendencies derived from the three-dimensional radar reflectivity data, where available. To further refine initial conditions for the convection-allowing HRRR model, a similar procedure is used in the HRRR, but without DFI. Both of these procedures, together called the "Radar-LHI" (latent heating initialization) technique, have been essential for initialization of ongoing precipitation systems, especially convective systems, within all NOAA operational versions of the 13-km RAP and 3-km HRRR models extending through the latest implementation upgrade at NCEP in 2020. Application of the latent heat-derived temperature tendency induces a vertical circulation with low-level convergence and upper-level divergence in precipitation systems. Retrospective tests of the Radar-LHI technique show significant improvement in short-range (0-6 h) precipitation system forecasts, as revealed by reflectivity verification scores. Results presented document the impact on HRRR reflectivity forecasts of the radar reflectivity initialization technique applied to the RAP alone, HRRR alone, and both the RAP and HRRR. SIGNIFICANCE STATEMENT: The large forecast uncertainty of convective situations, even at short lead times, coupled with the hazardous weather they produce, makes convective storm prediction one of the most significant shortrange forecast challenges confronting the operational numerical weather prediction community. Prediction of heavy precipitation events also requires accurate initialization of precipitation systems. An innovative assimilation technique using radar reflectivity data to initialize NOAA operational weather prediction models is described. This technique, which uses latent heating specified from radar reflectivity (and can accommodate lightning data and other convection/precipitation indicators), was first implemented in 2009 at NOAA/NCEP and continues to be used in 2022 in the NCEP-operational RAP and HRRR models, making it a backbone of the NOAA rapidly updated numerical weather prediction capability. [ABSTRACT FROM AUTHOR]

The High-Resolution Rapid Refresh (HRRR) is a convection-allowing implementation of the Advanced Research version of the Weather Research and Forecasting (WRF-ARW) Model with hourly data assimilation that covers the conterminous United States and Alaska and runs in real time at the NOAA/National Centers for Environmental Prediction (NCEP). Implemented operationally at NOAA/NCEP in 2014, the HRRR features 3-km horizontal grid spacing and frequent forecasts (hourly for CONUS and 3-hourly for Alaska). HRRR initialization is designed for optimal short-range forecast skill with a particular focus on the evolution of precipitating systems. Key components of the initialization are radar-reflectivity data assimilation, hybrid ensemble-variational assimilation of conventional weather observations, and a cloud analysis to initialize stratiform cloud layers. From this initial state, HRRR forecasts are produced out to 18 h every hour, and out to 48 h every 6 h, with boundary conditions provided by the Rapid Refresh system. Between 2014 and 2020, HRRR development was focused on reducing model bias errors and improving forecast realism and accuracy. Improved representation of the planetary boundary layer, subgrid-scale clouds, and land surface contributed extensively to overall HRRR improvements. The final version of the HRRR (HRRRv4), implemented in late 2020, also features hybrid data assimilation using flow-dependent covariances from a 3-km, 36-member ensemble ("HRRRDAS") with explicit convective storms. HRRRv4 also includes prediction of wildfire smoke plumes. The HRRR provides a baseline capability for evaluating NOAA's next-generation Rapid Refresh Forecast System, now under development. SIGNIFICANCE STATEMENT: NOAA's operational hourly updating, convection-allowing model, the High-Resolution Rapid Refresh (HRRR), is a key tool for short-range weather forecasting and situational awareness. Improvements in assimilation of weather observations, as well as in physics parameterizations, have led to improvements in simulated radar reflectivity and quantitative precipitation forecasts since the initial implementation of HRRR in September 2014. Other targeted development has focused on improved representation of the diurnal cycle of the planetary boundary layer, resulting in improved near-surface temperature and humidity forecasts. Additional physics and data assimilation changes have led to improved treatment of the development and erosion of low-level clouds, including subgrid-scale clouds. The final version of HRRR features storm-scale ensemble data assimilation and explicit prediction of wildfire smoke plumes. [ABSTRACT FROM AUTHOR]

The High-Resolution Rapid Refresh (HRRR) is a convection-allowing implementation of the Advanced Research version of the Weather Research and Forecast (WRF-ARW) Model that covers the conterminous United States and Alaska and runs hourly (for CONUS; every 3 h for Alaska) in real time at the National Centers for Environmental Prediction. The high-resolution forecasts support a variety of user applications including aviation, renewable energy, and prediction of many forms of severe weather. In this second of two articles, forecast performance is documented for a wide variety of forecast variables and across HRRR versions. HRRR performance varies across geographical domain, season, and time of day depending on both prevalence of particular meteorological phenomena and the availability of both conventional and nonconventional observations. Station-based verification of surface weather forecasts (2-m temperature and dewpoint temperature, 10-m winds, visibility, and cloud ceiling) highlights the ability of the HRRR to represent daily planetary boundary layer evolution and the development of convective and stratiform cloud systems, while gridded verification of simulated composite radar reflectivity and quantitative precipitation forecasts reveals HRRR predictive skill for summer and winter precipitation systems. Significant improvements in performance for specific forecast problems are documented for the upgrade versions of the HRRR (HRRRv2, v3, and v4) implemented in 2016, 2018, and 2020, respectively. Development of the HRRR model data assimilation and physics paves the way for future progress with operational convectivescale modeling. SIGNIFICANCE STATEMENT: NOAA's operational hourly updating convection-allowing model, the High-Resolution Rapid Refresh (HRRR), is a key tool for short-range weather forecasting and situational awareness. Improvements in assimilation of weather observations, as well as in physics parameterizations, has led to improvements in simulated radar reflectivity and quantitative precipitation forecasts since the initial implementation of HRRR in September 2014. Other targeted development has focused on improved representation of the diurnal cycle of the planetary boundary layer, resulting in improved near-surface temperature and humidity forecasts. Additional physics and data assimilation changes have led to improved treatment of the development and erosion of low-level clouds, including subgrid-scale clouds. The final version of HRRR features storm-scale ensemble data assimilation and explicit prediction of wildfire smoke plumes. [ABSTRACT FROM AUTHOR]

Levofloxacin (LFX) is a highly effective anti-tuberculosis drug with a pronounced bactericidal activity against Mycobacterium tuberculosis (Mtb). In this work, an "organic solvent-free" approach has been used for the development of polylactic-co-glycolic acid (PLGA) microparticles and scaffolds containing LFX at a therapeutically significant concentration, providing for its sustained release. To achieve the target, both nonpolar supercritical carbon dioxide and polar supercritical trifluoromethane have been used. By changing the composition, surface morphology, size, and internal structure of the polymer carriers, one can control the kinetics of the LFX release into phosphate buffered saline solutions and physiological media, providing for its acceptable burst and desirable concentration in the prolonged phase. The biocompatibility and bactericidal efficacy of PLGA/LFX carriers assessed both in vitro (against Mtb phagocytosed by macrophages) and in vivo (against inbred BALB/c mice aerogenically infected with Mtb) demonstrated their anti-tuberculosis activity comparable with that of the standard daily intragastric levofloxacin administration. These results make it possible to consider the developed compositions as a promising candidate for anti-tuberculosis control release formulations providing for the further evaluation of their activity against Mtb and their metabolism in vivo over long periods of tuberculosis infection. [ABSTRACT FROM AUTHOR]

Initialization methods are needed for geophysical components of Earth system prediction models. These methods are needed from medium-range to decadal predictions and also for short-range Earth system forecasts in support of safety (e.g., severe weather), economic (e.g., energy), and other applications. Strongly coupled land–atmosphere data assimilation (SCDA), producing balanced initial conditions across the land–atmosphere components, has not yet been introduced to operational numerical weather prediction (NWP) systems. Most NWP systems have evolved separate data assimilation (DA) procedures for the atmosphere versus land/snow system components. This separated method has been classified as a weakly coupled DA system (WCDA). In the NOAA operational short-range weather models, a moderately coupled land–snow–atmosphere assimilation method (MCLDA) has been implemented, a step forward from WCDA toward SCDA. The atmosphere and land (including snow) variables are both updated within the DA using the same set of observations (aircraft, radiosonde, satellite radiances, surface, etc.). Using this assimilation method, land surface state variables have cycled continuously for 6 years since 2015 for the 3-km NOAA HRRR model and with CONUS cycling since 1997. Month-long experiments were conducted with and without MCLDA for both winter and summer seasons using the 13-km Rapid Refresh model with atmosphere (50 levels), soil (9 levels), and snow (up to 2 layers if present) on the same horizontal grid. Improvements were evident for 2-m temperature for all times of day out to 6–12 h for both seasons but stronger in winter. Better temperature forecasts were also shown in the 1000–900-hPa layer corresponding roughly to the boundary layer. Significance Statement: Accuracy of weather models depends on accurate initial conditions for soil temperature and moisture as well as for the atmosphere itself. This paper describes a moderately coupled data assimilation method that modifies soil conditions based on forecast error corrections indicated by atmospheric observations. This method has been tested for a month-long period in summer and winter and shown to consistently improve short-range forecasts of 2-m temperature and moisture. This coupled data assimilation method is used already in NOAA operational short-range models to improve its prediction skill for clouds, convective storms, and general weather conditions. [ABSTRACT FROM AUTHOR]

Application of lake models coupled within earth-system prediction models, especially for short-term predictions from days to weeks, requires accurate initialization of lake temperatures. Here, we describe a lake initialization method by cycling within an hourly updated weather prediction model to constrain lake temperature evolution. We compare these simulated lake temperature values with other estimates from satellite and in situ and interpolated-SST data sets for a multi-month period in 2021. The lake cycling initialization, now applied to two operational US NOAA weather models, was found to decrease errors in lake temperature from as much as 5-10 K (using interpolated-SST data) to about 1-2 K (comparing with available in situ and satellite observations. [ABSTRACT FROM AUTHOR]

Subgrid variability of snow is important in studying surface‐atmosphere interactions as it affects grid‐scale processes. However, this dynamic variability is currently not well‐represented in most land‐surface models (LSMs). A stochastic snow model using the Fokker‐Planck Equation (FPE) has been developed specifically for representing subgrid variability of snow. In this study, the FPE snow model was coupled into the Rapid Update Cycle (RUC) LSM, which provides lower boundary conditions for the operational NOAA Rapid Refresh and High‐Resolution Rapid Refresh weather prediction systems. This coupled land‐surface model with subgrid snow processes is named the RUC‐Stochastic Snow (SS) LSM. The performance of RUC‐SS LSM and RUC LSM is analyzed in detail in initial offline single‐column testing over two 13‐km × 13‐km grid cells. The results show that RUC‐SS LSM has a much better capability in estimating snow cover fraction than RUC LSM, especially during the late part of the snow‐melting season. The simulated duration of the partially snow‐covered period at the snow‐melting stage is extended from a few days with RUC LSM to about one month with the RUC‐SS LSM, thus improving prediction of surface energy budget components. For example, the latent and sensible heat fluxes and skin temperature increase gradually in the RUC‐SS LSM during the transition from full coverage to snow‐free conditions. In contrast, this transition happens too quickly in RUC LSM, and the energy‐budget components change too abruptly. The results of RUC‐SS demonstrate its capability to account for subgrid variability of snow cover and show that considering subgrid variability of snow in LSMs is important for simulating surface‐atmosphere interactions. Key Points: A stochastic snow model is integrated into a land‐surface model to represent snow subgrid variability and its change over timeThis subgrid snow treatment is tested in an offline mode of the land‐surface model to demonstrate its effectivenessThe effects of snow subgrid variability on energy components and skin temperature in land‐surface models are quantified [ABSTRACT FROM AUTHOR]

Lake-effect convective snowstorms frequently produce high-impact, hazardous winter weather conditions downwind of the North American Great Lakes. During lake-effect snow events, the lake surfaces can cool rapidly, and in some cases, notable development of ice cover occurs. Such rapid changes in the lake-surface conditions are not accounted for in existing operational weather forecast models, such as the National Oceanic and Atmospheric Administration's (NOAA) High-Resolution Rapid Refresh (HRRR) model, resulting in reduced performance of lake-effect snow forecasts. As a milestone to future implementations in the Great Lakes Operational Forecast System (GLOFS) and HRRR, this study examines the one-way linkage between the hydrodynamic-ice model [the Finite-Volume Community Ocean Model coupled with the unstructured grid version of the Los Alamos Sea Ice Model (FVCOM-CICE), the physical core model of GLOFS] and the atmospheric model [the Weather Research and Forecasting (WRF) Model, the physical core model of HRRR]. The realistic representation of lake-surface cooling and ice development or its fractional coverage during three lake-effect snow events was achieved by feeding the FVCOM-CICE simulated lake-surface conditions to WRF (using a regional configuration of HRRR), resulting in the improved simulation of the turbulent heat fluxes over the lakes and resulting snow water equivalent in the downwind areas. This study shows that the one-way coupling is a practical approach that is well suited to the operational environment, as it requires little to no increase in computational resources yet can result in improved forecasts of regional weather and lake conditions. [ABSTRACT FROM AUTHOR]

Subgrid variability of solar downward radiation at the surface can be important in estimating subgrid variability of other radiation‐driven variables, such as snowmelt and soil temperature. However, this information is ignored in current hydrological and weather prediction models as only the mean downward solar radiation of model grid is used. In this study, a parameterization for estimating subgrid variability of downward solar radiation from the model grid mean using high‐resolution digital elevation model (DEM) data is proposed. This scheme considers aspect and slope effects on the subgrid variability. The advantage of this scheme is that computations are performed at the same resolution as the considered hydrological or weather prediction model, and subgrid topographic properties derived from high‐resolution DEM data are used as static inputs. This proposed scheme has been verified in mountainous and flat areas, respectively. It is found that the scheme can well estimate the subgrid variability of downward solar radiation. Also, effects of the DEM resolution on the calculated subgrid variability and the spatial correlation of downward solar radiation are studied. Results show that modeled subgrid variability highly depends on the resolution of the DEM, while the spatial correlation is negligibly time dependent. The proposed scheme can be used in any hydrological and weather prediction model to estimate subgrid variability of downward solar radiation. For example, it is planned to be tested in future NOAA regional and global weather models to account for the effects of the subgrid variability of downward solar radiation on the snow model of the land‐surface component. Key Points: Subgrid variability of downward solar radiation and subgrid variability of terrain are connected in a straightforward way in this studyThe proposed scheme provides an effective approach to include high‐resolution topographic information in a coarse model grid cellThe proposed scheme can be used in any hydrological or land‐surface model to estimate variance of downward solar radiation [ABSTRACT FROM AUTHOR]

A stochastic parameter perturbation (SPP) scheme consisting of spatially and temporally varying perturbations of uncertain parameters in the Grell-Freitas convective scheme and the Mellor-Yamada-Nakanishi- Niino planetary boundary scheme was developed within the Rapid Refresh ensemble system based on the Weather Research and Forecasting Model. Alone the stochastic parameter perturbations generate insufficient spread to be an alternative to the operational configuration that utilizes combinations of multiple parameterization schemes. However, when combined with other stochastic parameterization schemes, such as the stochastic kinetic energy backscatter (SKEB) scheme or the stochastic perturbation of physics tendencies (SPPT) scheme, the stochastic ensemble system has comparable forecast performance. An additional analysis quantifies the added value of combining SPP and SPPT over an ensemble that uses SPPT only, which is generally beneficial, especially for surface variables. The ensemble combining all three stochastic methods consistently produces the best spread-skill ratio and generally outperforms the multiphysics ensemble. The results of this study indicate that using a single-physics suite ensemble together with stochastic methods is an attractive alternative to multiphysics ensembles and should be considered in the design of future high-resolution regional and global ensembles. [ABSTRACT FROM AUTHOR]

The land surface model (LSM) described in this manuscript was originally developed as part of the NOAA Rapid Update Cycle (RUC) model development effort; with ongoing modifications, it is now used as an option for the WRF community model. The RUC model and its WRF-based NOAA successor, the Rapid Refresh (RAP), are hourly updated and have an emphasis on short-range, near-surface forecasts including aviation-impact variables and preconvective environment. Therefore, coupling to this LSM (hereafter the RUC LSM) has been critical to provide more accurate lower boundary conditions. This paper describes changes made to the RUC LSM since earlier descriptions, including extension from six to nine levels, improved snow treatment, and new land-use data from MODIS. The RUC LSM became operational at the NOAA/National Centers for Environmental Prediction (NCEP) as part of the RUC from 1998-2012 and as part of the RAP from 2012 through the present. The simple treatments of basic land surface processes in the RUC LSM have proven to be physically robust and capable of realistically representing the evolution of soil moisture, soil temperature, and snow in cycled models. Extension of the RAP domain to encompass all of North America and adjacent high-latitude ocean areas necessitated further development of the RUC LSM for application in the tundra permafrost regions and over Arctic sea ice. Other modifications include refinements in the snow model and a more accurate specification of albedo, roughness length, and other surface properties. These recent modifications in the RUC LSM are described and evaluated in this paper. [ABSTRACT FROM AUTHOR]

The Rapid Refresh (RAP) and High-Resolution Rapid Refresh (HRRR), both operational at NOAA's National Centers for Environmental Prediction (NCEP) use the Thompson et al. mixed-phase bulk cloud microphysics scheme. This scheme permits predicted surface precipitation to simultaneously consist of rain, snow, and graupel at the same location under certain conditions. Here, the explicit precipitation-type diagnostic method is described as used in conjunction with the Thompson et al. scheme in the RAP and HRRR models. The postprocessing logic combines the explicitly predicted multispecies hydrometeor data and other information from the model forecasts to produce fields of surface precipitation type that distinguish between rain and freezing rain, and to also portray areas of mixed precipitation. This explicit precipitation-type diagnostic method is used with the NOAA operational RAP and HRRR models. Verification from two winter seasons from 2013 to 2015 is provided against METAR surface observations. An example of this product from a January 2015 south-central United States winter storm is also shown. [ABSTRACT FROM AUTHOR]

The Rapid Refresh (RAP), an hourly updated assimilation and model forecast system, replaced the Rapid Update Cycle (RUC) as an operational regional analysis and forecast system among the suite of models at the NOAA/National Centers for Environmental Prediction (NCEP) in 2012. The need for an effective hourly updated assimilation and modeling system for the United States for situational awareness and related decision-making has continued to increase for various applications including aviation (and transportation in general), severe weather, and energy. The RAP is distinct from the previous RUC in three primary aspects: a larger geographical domain (covering North America), use of the community-based Advanced Research version of the Weather Research and Forecasting (WRF) Model (ARW) replacing the RUC forecast model, and use of the Gridpoint Statistical Interpolation analysis system (GSI) instead of the RUC three-dimensional variational data assimilation (3DVar). As part of the RAP development, modifications have been made to the community ARW model (especially in model physics) and GSI assimilation systems, some based on previous model and assimilation design innovations developed initially with the RUC. Upper-air comparison is included for forecast verification against both rawinsondes and aircraft reports, the latter allowing hourly verification. In general, the RAP produces superior forecasts to those from the RUC, and its skill has continued to increase from 2012 up to RAP version 3 as of 2015. In addition, the RAP can improve on persistence forecasts for the 1-3-h forecast range for surface, upper-air, and ceiling forecasts. [ABSTRACT FROM AUTHOR]

Because of limitations of variational and ensemble data assimilation schemes, resulting analysis fields exhibit some noise from imbalance in subsequent model forecasts. Controlling finescale noise is desirable in the NOAA's Rapid Refresh (RAP) assimilation/forecast system, which uses an hourly data assimilation cycle. Hence, a digital filter initialization (DFI) capability has been introduced into the Weather Research and Forecasting Model and applied operationally in the RAP, for which hourly intermittent assimilation makes DFI essential. A brief overview of the DFI approach, its implementation, and some of its advantages are discussed. Results from a 1-week impact test with and without DFI demonstrate that DFI is effective at reducing high-frequency noise in short-term operational forecasts as well as providing evidence of reduced errors in the 1-h mass and momentum fields. However, DFI is also shown to reduce the strength of parameterized deep moist convection during the first hour of the forecast. [ABSTRACT FROM AUTHOR]

Various genetic markers such as IS-elements, DR-elements, variable number tandem repeats (VNTR), single nucleotide polymorphisms (SNPs) in housekeeping genes and other groups of genes are being used for genotyping. We propose a different approach. We suggest the type II toxin-antitoxin (TA) systems, which play a significant role in the formation of pathogenicity, tolerance and persistence phenotypes, and thus in the survival of Mycobacterium tuberculosis in the host organism at various developmental stages (colonization, infection of macrophages, etc.), as the marker genes. Most genes of TA systems function together, forming a single network: an antitoxin from one pair may interact with toxins from other pairs and even from other families. In this work a bioinformatics analysis of genes of the type II TA systems from 173 sequenced genomes of M. tuberculosis was performed. A number of genes of type II TA systems were found to carry SNPs that correlate with specific genotypes. We propose a minimally sufficient set of genes of TA systems for separation of M. tuberculosis strains at nine basic genotype and for further division into subtypes. Using this set of genes, we genotyped a collection consisting of 62 clinical isolates of M. tuberculosis. The possibility of using our set of genes for genotyping using PCR is also demonstrated. [ABSTRACT FROM AUTHOR]

A mesoscale atmospheric forecast model configured in a hybrid isentropic–sigma vertical coordinate and used in the NOAA Rapid Update Cycle (RUC) for operational numerical guidance is presented. The RUC model is the only quasi-isentropic forecast model running operationally in the world and is distinguished from other hybrid isentropic models by its application at fairly high horizontal resolution (10–20 km) and a generalized vertical coordinate formulation that allows model levels to remain continuous and yet be purely isentropic well into the middle and even lower troposphere. The RUC model is fully described in its 2003 operational version, including numerics and physical parameterizations. The use of these parameterizations, including mixed-phase cloud microphysics and an ensemble-closure-based cumulus parameterization, is fully consistent with the RUC vertical coordinate without any loss of generality. A series of experiments confirm that the RUC hybrid θ–σ coordinate reduces cross-coordinate transport over a quasi-horizontal σ coordinate. This reduction in cross-coordinate vertical transport results in less numerical vertical diffusion and thereby improves numerical accuracy for moist reversible processes. Finally, a forecast is presented of a strong cyclogenesis case over the eastern United States in which the RUC model produced an accurate 36-h prediction, especially in a 10-km nested version. Horizontal and vertical plots from these forecasts give evidence of detailed yet coherent structures of potential vorticity, moisture, and vertical motion. [ABSTRACT FROM AUTHOR]

The Rapid Update Cycle (RUC), an operational regional analysis–forecast system among the suite of models at the National Centers for Environmental Prediction (NCEP), is distinctive in two primary aspects: its hourly assimilation cycle and its use of a hybrid isentropic–sigma vertical coordinate. The use of a quasi-isentropic coordinate for the analysis increment allows the influence of observations to be adaptively shaped by the potential temperature structure around the observation, while the hourly update cycle allows for a very current analysis and short-range forecast. Herein, the RUC analysis framework in the hybrid coordinate is described, and some considerations for high-frequency cycling are discussed. A 20-km 50-level hourly version of the RUC was implemented into operations at NCEP in April 2002. This followed an initial implementation with 60-km horizontal grid spacing and a 3-h cycle in 1994 and a major upgrade including 40-km horizontal grid spacing in 1998. Verification of forecasts from the latest 20-km version is presented using rawinsonde and surface observations. These verification statistics show that the hourly RUC assimilation cycle improves short-range forecasts (compared to longer-range forecasts valid at the same time) even down to the 1-h projection. [ABSTRACT FROM AUTHOR]

This study compares three modifications to the one-dimensional planetary boundary layer scheme that is implemented in the s-- u hybrid-b version of the Mesoscale Analysis and Prediction System (MAPS) and the Rapid Update Cycle (RUC). All three modifications are based on the incorporation of a simple soil model into the basic version to more accurately calculate the moisture and heat fluxes across the ground surface. The presented schemes are of increasing sophistication: the first model combines the soil model with heat and moisture budget equations for the ground surface and uses an explicit numerical scheme to compute the surface fluxes; the second model uses a more energy-conservative implicit solution for the latent and sensible surface fluxes and heat and moisture soil fluxes; the third model further incorporates a simple parameterization of the evapo-transpiration process. The comparison includes the effect of different schemes on diurnal changes of surface temperature and soil heat flux. The schemes are tested for two case studies; a dry case from the O'Neill, Nebraska, Great Plains Turbulence Field Program and a moist case from the First ISLSCP (International Satellite Land Surface Cli-matology Project) Field Experiment. Tests are performed to evaluate sensitivity to soil parameters related to thermal diffusivity and to vertical resolution of the soil scheme. Overall, the comparison supports the idea that implementation of a multilevel soil model is competitive with and can even improve the ground surface tem-perature forecast over that produced by the present MAPS implementation of the force restore method. The case study demonstrates that incorporation of a primitive evapotranspiration model can give positive results. [ABSTRACT FROM AUTHOR]

Carbocyclic nucleosides have long played a role in antiviral, antiparasitic, and antibacterial therapies. Recent results from our laboratories from two structurally related scaffolds have shown promising activity against both Mycobacterium tuberculosis and several parasitic strains. As a result, a small structure activity relationship study was designed to further probe their activity and potential. Their synthesis and the results of the subsequent biological activity are reported herein. [ABSTRACT FROM AUTHOR]

A series of novel 5′-norcarbocyclic derivatives of 5-alkoxymethyl or 5-alkyltriazolyl-methyl uracil were synthesized and the activity of the compounds evaluated against both Gram-positive and Gram-negative bacteria. The growth of Mycobacterium smegmatis was completely inhibited by the most active compounds at a MIC99 of 67 μg/mL (mc2155) and a MIC99 of 6.7–67 μg/mL (VKPM Ac 1339). Several compounds also showed the ability to inhibit the growth of attenuated strains of Mycobacterium tuberculosis ATCC 25177 (MIC99 28–61 μg/mL) and Mycobacterium bovis ATCC 35737 (MIC99 50–60 μg/mL), as well as two virulent strains of M. tuberculosis; a laboratory strain H37Rv (MIC99 20–50 μg/mL) and a clinical strain with multiple drug resistance MS-115 (MIC99 20–50 μg/mL). Transmission electron microscopy (TEM) evaluation of M. tuberculosis H37Rv bacterial cells treated with one of the compounds demonstrated destruction of the bacterial cell wall, suggesting that the mechanism of action for these compounds may be related to their interactions with bacteria cell walls. [ABSTRACT FROM AUTHOR]