1. RT-PCR-based cytochrome P450 expression profile of oral tissue samples.
- Author
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Sarikaya, D., Chiba, I., Bilgen, C., KamatakiI, T., and Topcu, Z.
- Subjects
MESSENGER RNA ,BIOCHEMISTRY ,RNA ,POLYMERASE chain reaction ,POLYMERIZATION - Abstract
Objective: To analyse the expression of individual forms of cytochrome P450 (CYP) at the mRNA level in five homogenized oral buccal tissue samples from four individuals with or without oral malignancy. Method: Individual forms of CYPs were studied by reverse transcriptase-polymerase chain reaction (RT-PCR), using specific primers for CYPs 2B6, 2C, 2D6, 2E1, 3A3/4 and 3A5, and oral CYP expressions were compared with CYP expression in liver tissue. Results: Consistent expression of CYPs 2C, 2E1 and 3A5 was observed in oral buccal tissue at mRNA level. Conclusions: These particular CYPs have possible roles in the protection of the body against orally ingested xenobiotics as well as influence the bioavailability of therapeutic compounds. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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