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Objective: To evaluate the effect of biological control agents (BCA) and two biological formulations (Bio-Terra and Bio-CNPR) against the phytopathogens found in the soil of huacle chili (Capsicum annuum) plantations, in order to obtain healthy seedlings. Design/Methodology/Approach: The following design variables were evaluated: germination (%), disease incidence (%), and survival (%). Stem height and root length were also evaluated. The experimental unit was a tray with n=30 seedlings per each treatments (T0, T1, T2, T3, and T4) and four repetitions. An analysis of variance (ANOVA) was used. Tukey's mean test was applied (using the Minitab Statistical program version 20.0) in units that presented differences (p ≤ 0.05). Results: The phytopathogenic microorganism Fusarium spp. was isolated from the soil of the plots cultivated with huacle chili. The T1 treatment recorded the best inhibitory effect against Fusarium spp.: it had 92.2% germination, 18.3% incidence of Fusarium spp., and 82% surviving seedlings (with an average height of 16 cm and a root length of 7.9 cm). The sterilization of the soil lead to T4 having the best results in germination, incidence, and survival, since Fusarium spp. did not damage huacle chili seedlings. In contrast, T0 did not prosper neither with BCA nor with biological products, since all the seedlings in non-sterile soil died. Study Limitations/Implications: A plastic barrier was placed between the trays with the chili seedlings to avoid cross-contamination between treatments. Findings/Conclusions: T1 (the BCA with nitrogen-fixing bacteria and B. subtilis) recorded the best counteracting results against the damage generated by Fusarium spp. Their antagonism allowed a high percentage of survival of huacle chili seedlings and encouraged plant development through the best root growth and height of the seedlings. [ABSTRACT FROM AUTHOR]

Common bean rust, Bean golden yellow mosaic virus (BGYMV), Bean common mosaic virus (BCMV), and Bean common mosaic necrosis virus (BCMNV) are devastating diseases of dry bean (Phaseolus vulgaris L.) in Central America and the Caribbean. The development of dry bean cultivars with enhanced levels of genetic resistance to these and other diseases is an important goal of the University of Puerto Rico (UPR) and other dry bean breeding programs in Central America and the Caribbean. PR1572‐19 (Reg. no. GP‐308, PI 692976) and PR1572‐26 (Reg. no. GP‐307, PI 692975) are multiple virus‐ and rust‐resistant pinto bean germplasm lines adapted to the humid tropics that were developed and released cooperatively in 2019 by the UPR Agricultural Experiment Station, the Instituto Dominicano de Investigaciones Agropecuarias y Forestales, and the USDA–ARS. Both PR1572‐19 and PR1572‐26 possess the bgm‐1 gene for resistance to BGYMV, the I and bc‐3 loci that provide broad resistance to all strains of BCMV and BCMNV in the United States and the Caribbean, and the Ur‐3 and Ur‐11 loci that confer comprehensive resistance to all known races of the bean rust pathogen. PR1572‐26 also has the SW12 quantitative trait locus marker associated with resistance to BGYMV. PR1572‐19 and PR1572‐26 produced mean seed yields similar to the check cultivar 'Santa Fe' in trials conducted in Puerto Rico and mean seed yields comparable to the pinto cultivar 'La Paz' in the Cooperative Dry Bean Nursery conducted in Puerto Rico. Both lines should serve as useful sources of resistance to BGYMV, BCMV, BCMNV, and bean rust for common beans of the Durango race that includes the pinto and great northern market classes. [ABSTRACT FROM AUTHOR]

Investigates the presence of bovine sperm protease, 66 kDa (BSp66) in hamster spermatozoa using heterologous antibodies against bovine BSp66 and its role during in vitro fertilization. Sperm isolation; Assessment of sperm motility and viability; Location of BSp66 in sperm from cauda and caput epididymis.

This article presents information on the registration of Carrizalito small red bean. Small red dry bean Carrizalito was developed at the Escuela Agricola Panamericana in Zamorano, Honduras, and released for Honduras in 2003 and Costa Rica in 2004, in collaboration with the National Bean Programs of Honduras and Costa Rica, and the University of Puerto Rico. Carrizalito is a high yielding, disease resistant cultivar, adapted to the midaltitude bean production regions of Central America. In 2004, Carrizalito was mechanically inoculated at the University of Puerto Rico with the NL3 strain of Bean common mosaic necrotic virus (BCMV) and found to have top necrosis caused by the presence of the dominant I gene for resistance to BCMV. Ashy stem blight is favored by warm dry conditions associated with drought stress. Seedlings of Carrizalito were inoculated in the greenhouse at the UPR with the ashy stem blight pathogen Macrophominaphaseolina and found to be resistant. Carrizalito has an indeterminate upright, Type III growth habit with a short vine. Carrizalito flowers in 35 to 37 d and matures in 68 to 70 d.

Presents information on the `Tio Canela-75,' a small red bean (Phaseolus vulgaris) (registration number CV-139, PI 595892). Who developed and released Tio Canela-75; Detailed information on Tio Cnaela-75 including study conducted; Production on breeder seed of Tio Canela-75.