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The study objective was to evaluate the interaction between corticotrophin releasing factor (CRF) receptor signaling and prophylactic antibiotic administration on intestinal physiology in newly weaned and transported pigs. Pigs (n = 56; 5.70 ± 1.05 kg) were weaned (20.49 ± 0.64 d), a blood sample was taken, and then pigs were given an intraperitoneal injection of saline (SAL; n = 28 pigs) or a CRF receptor antagonist (CRFA; n = 28 pigs; 30 µg/kg body weight; Astressin B), and then were transported in a livestock trailer for 12 h and 49 min. A second and third intraperitoneal injection was given at 4 h 42 min and 11 h 36 min into the transport process, respectively. Following transport, 4 SAL and 4 CRFA pigs were blood sampled and euthanized. The remaining 48 pigs were individually housed and given dietary antibiotics [AB; n = 12 SAL and 12 CRFA pigs; chlortetracycline (441 ppm) + tiamulin (38.6 ppm)] or no dietary antibiotics (NAB; n = 12 SAL and 12 CRFA pigs) for 14 d post-transport. Blood was collected at 12 h and on d 3, 7, and 14, and then pigs were euthanized on d 7 (n = 24) and d 14 (n = 24) post-weaning and transport. Circulating cortisol was reduced (p = 0.05) in CRFA pigs when compared to SAL pigs post-weaning and transport. On d 7, jejunal villus height and crypt depth was greater overall (p < 0.05) in AB-fed pigs versus NAB-fed pigs. On d 14, ileal crypt depth was reduced (p = 0.02) in CRFA pigs when compared to SAL pigs. Jejunal CRF mRNA abundance tended to be reduced (p = 0.09) on d 7 in CRFA pigs versus SAL pigs. On d 14, jejunal tumor necrosis factor-alpha was reduced (p = 0.01) in AB-fed pigs versus NAB-fed pigs. On d 7, change in glucose short-circuit current tended to be increased (p = 0.07) in CRFA pigs fed the AB diet when compared to CRFA pigs fed the NAB diet. In conclusion, CRFA pigs and pigs fed AB had some similar biological intestinal function measures post-weaning and transport. [ABSTRACT FROM AUTHOR]

Domesticated sows are motivated to perform nesting behavior prior to farrowing, and nesting material can reduce piglet cold stress. However, nesting material may not be practical in most production systems due to the potential for clogging slurry systems. Therefore, the study objectives were to assess an alternative nesting material provided prior to farrowing on sow welfare and piglet survival, and to investigate the effect of the entire nesting environment on piglet survival and growth performance. We hypothesized that the provision of jute nesting material would decrease sow stress and farrowing duration, and that nesting mat provision would allow piglets to remain euthermic and improve survival and growth. Sows (N  = 20) were randomly assigned to one of two treatments: a farrowing crate with three pieces of 40.6 × 21.6 cm jute nesting material (Nest; n  = 10) and two enriched piglet mats made from an acrylic board (28.0 × 86.4 cm) covered with a microfiber material, or a farrowing crate without nesting material (Control; n  = 10) and one standard plastic piglet mat (28.0 × 86.4 cm). Jute pieces were attached to the front of the crate to prevent substrate from falling through the slatted floors. Saliva samples were collected to measure cortisol and immunoglobulin A (IgA), on days −1, 0, 1, and 2 relative to farrowing, and a final sample was collected at weaning (day 16.9 ± 0.18). Blood was collected from four piglets per litter to measure immunoglobulin G (IgG) at 48 h, day 7, and weaning. Piglet skin temperature (T S) was measured on two piglets per litter using an infrared camera for 3 d after birth at 0800, 1200, 1600, and 2000 h. One piglet was randomly chosen from the heat lamp and nonheat lamp side of the crate to measure T S. Video was continuously coded for observations of jute- and crate-directed interactions. Data were analyzed as a mixed model analysis of variance in SAS 9.4. Nest sows performed less crate-directed behavior than Control sows (P  = 0.02). Cortisol tended to be reduced in Nest sows (P  = 0.08) when compared to Controls, but no differences in IgA concentrations (P  > 0.40) were detected. Nest piglets tended to be heavier on day 7 (P  < 0.10), had greater IgG concentrations (P  = 0.03), and had greater T S (P  = 0.02) versus Controls. No farrowing duration or number of stillbirth differences were observed (P  > 0.70). The jute material and piglet nests positively impacted sow welfare and piglet measures but did not translate into improved piglet survival. [ABSTRACT FROM AUTHOR]

A total of 606 sows (PIC 1050) and their progeny (PIC 1050 × 280) were used to determine if feeding gestating and lactating sows a proprietary strain of Pichia guilliermondii as a whole-cell inactivated yeast product (WCY ; CitriStim, ADM Animal Nutrition, Quincy, IL) improves sow and litter performance in a commercial production system. Once confirmed pregnant at d 35 post-breeding pregnancy check, sows were fed a basal gestation control (CON) diet (0.55% SID lysine) or the control diet fortified with 0.15% of the WCY replacing corn in the CON diet. Dietary treatments were also fed in lactation (1.05% SID lysine) once sows were moved into farrowing crates on approximately d 112 of gestation until weaning. Sows supplemented with WCY in gestation and lactation had increased total born piglets by 0.45 pigs (P < 0.04), piglets born alive (14.27 vs. 13.85; P < 0.04), and, therefore, heavier born alive litter weights (P < 0.001) compared to CON fed sows. A greater post cross-foster litter size (P < 0.001) meant that litter size at weaning was increased by 0.54 pigs when sows were fed WCY compared to CON (P < 0.001). However, litter weaning weights and 21-d adjusted litter weaning weights were similar (P > 0.158), although numerically greater, for WCY fed sows. Pigs from CON fed sows were 0.35 kg heavier at weaning compared to pigs from WCY fed sows (P < 0.001). This increase in weaning weight of pigs from CON fed sows is partially explained by their 0.93 d longer lactation (P < 0.001) and may also be due to the smaller litter size throughout lactation. The percent of litters treated for scours decreased from 38.3 to 14.2% when sows were fed WCY (P < 0.001). The distribution of birth and weaning weights was not impacted (P > 0.2461) by treatment. In conclusion, feeding gestating and lactating sows a proprietary strain of Pichia guilliermondii as a whole-cell inactivated yeast product increased the number of pigs born and weaned, and decreased the prevalence of scours during lactation. [ABSTRACT FROM AUTHOR]

The American swine industry relies on a model where facilities for farrowing are often geographically isolated from those used later for weanfinish, which necessitates multiple coincident stressors including weaning, mixing, transportation and feed/ water restriction. Previous studies indicate this combined stress reduces subsequent growth performance; however, the specific impact of each stressor on an average, objectively healthy piglet is not well understood, nor is the acute physiological response to this life event. The objective of this study was to determine the effect these stressors on thyroid function, intestinal health, and body weight (BW) in average, healthy pigs. In this study, a total population of 588 piglets were weighed the day prior to weaning and 230 piglets with the lowest Z-scores for BW were selected (5.74 ± 0.70 kg). Piglets (n = 70) were weaned directly into nursery pens and provided with feed and water, 70 were transported for 10 h with no access to feed and water, and 70 were grouped together into 2 large pens with no access to feed and water for an equivalent 8 h period. The remaining 20 piglets were not weaned and served as an unstressed control group. A total of 10 pigs from each group were euthanized at 32 and 56 h post-weaning for tissue sampling. Ileal villi height, crypt depth, and goblet cell numbers were measured, and no treatment effects observed (P > 0.05). Serum triiodothyronine (T3) concentrations were measured pre-treatment (t = 0), 24 h (t = 24), 48 h (t = 48), and immediately following euthanasia (t = 32 or 56 d). There were no significant differences in serum T3 among treatments at t = 0. At all-time points post stress event, control piglets had greater T3 concentrations compared with feed restricted (P < 0.05) and transported (P < 0.05) pigs. Weaned pigs at t = 32, t = 48, and t = 56 had decreased T3 concentrations (P < 0.05) and weaned pigs tended (P = 0.0584) to have decreased t = 24 T3 concentrations compared with pigs remaining with the sow. The remaining pigs (n = 50) per weaning group were weighed weekly throughout the nursery phase and every 3 wk in the grow-finish phase to determine the impact of each stressor on growth. There was no significant effect of weaning treatment on BW at any time point (P > 0.10). Carcass data including carcass weight, loin and backfat depths, and lean percentage were measured at time of slaughter with no significant differences found among groups (P > 0.60). As no effect on growth weight or carcass characteristics were observed between treatment groups in average pigs, this suggests that it may be beneficial to transport average pigs the longest distance, while minimizing transport for pigs on either end of the weaning weight spectrum. Further research is needed to compare effects of weaning and transportation stressors on average and weight extremes of weaned pigs. [ABSTRACT FROM AUTHOR]

Mixed sex pigs (n = 720) were placed in 12 rooms (Purdue Swine Environmental Research Building) to measure the effect of reduced crude protein (CP), amino acid (AA)-supplemented diets on growth and the carcass. Pigs were blocked by body weight (BW) and gender and allotted to room and pen (10 mixed-sex pigs/pen). Pigs were fed a nine-phase, wean-finish program. Control pigs consumed corn-soybean meal-distiller's dried grains with solubles (DDGS) diets containing no to minimal (Met) synthetic AA. The 2X diet was formulated to meet the seventh most-limiting AA, and balanced using synthetic AAs to meet all AA needs. The 1X diet was formulated to meet a CP value halfway between the control and 2X diet, and also balanced using synthetic AAs to meet all AA needs. Diets were formulated to identical net energy concentrations and balanced to meet standard ileal digestible NRC 2012 AA requirements. Pit vacuum samples were collected at the end of each growth phase for analyses of nitrogen, C and dry matter (DM). Pigs fed the Control and 1X diet grew faster (P < 0.005), had greater gain:feed (P < 0.001), and were heavier at market (P < 0.001) than animals fed the 2X diet. No consistent effects of diet were observed on average daily feed intake. Carcass data were analyzed for sex, diet and sex*diet effects. Reductions in dietary CP resulted in a linear reduction in ammonium nitrogen excretion per kg of BW gain in Nursery (P < 0.001) and Grow-Finish (P < 0.001) phases. Reductions in dietary CP, with synthetic AA supplementation resulted in a linear reduction in total nitrogen excreted per kg BW gain in the Grow-Finish phase (P < 0.001) and overall (P < 0.001). Total mineral excretion per kg gain was reduced in pigs fed 1X and 2X diets compared with control-fed pigs (P < 0.005). Reductions in dietary CP of ~3 and 5%-units from wean-finish result in reductions of total N excretion of 11.7 and 24.4%, respectively. Reduced performance and carcass characteristics observed in pigs fed the 2X diets indicates an inaccurate estimate of NRC 2012 AA requirements or ratios to lysine in a low CP diet. [ABSTRACT FROM AUTHOR]

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In utero heat stress alters postnatal physiological and behavioral stress responses in pigs. However, the mechanisms underlying these alterations have not been determined. The study objective was to characterize the postnatal hypothalamic–pituitary–adrenal axis response of in utero heat-stressed pigs. Pigs were subjected to a dexamethasone suppression test followed by a corticotrophin releasing hormone challenge at 10 and 15 weeks of age. Following the challenge, hypothalamic, pituitary, and adrenal tissues were collected from all pigs for mRNA abundance analyses. At 10 weeks of age, in utero heat-stressed pigs had a reduced (P < 0.05) cortisol response to the corticotrophin releasing hormone challenge versus controls. Additionally, the cortisol response tended to be greater overall (P < 0.10) in 15 versus 10-week-old pigs in response to the dexamethasone suppression test. The cortisol response tended to be reduced overall (P < 0.10) in 15 versus 10-week-old pigs in response to the corticotrophin releasing hormone challenge. Hypothalamic corticotropin releasing hormone mRNA abundance tended to be greater (P < 0.10) in in utero heat-stressed versus control pigs at 15-weeks of age. In summary, in utero heat stress altered some aspects of the hypothalamic–pituitary–adrenal axis related to corticotropin releasing hormone signaling, and age influenced this response. [ABSTRACT FROM AUTHOR]

Housing sows in groups create the challenge of decreasing fighting amongst sows. One proposed method to do so is to feed a high tryptophan diet, but the effect on the fetus is unknown. To investigate this, 66 sows were fed one of three diets: Control (0.14% SID tryptophan), Medium (0.28% SID tryptophan), or High (0.42% SID tryptophan), from days 28 to 35 of gestation. Sows gestated in standard gestation stalls. Blood samples were taken on day 27 prior to and on day 35 after tryptophan supplementation. On days 1, 2, and 3, nursing bouts were observed so as to record disputes and displacements from teat competition. The piglets' activity and fighting were recorded on days 3, 7, and 11 from 0700 to 1700 h. On day 12, four piglets per litter were blood sampled: two to be used in later behavior tests and two to act as controls for blood cortisol levels. On day 14, the two behavior test piglets from each litter were subjected to a 10-min Isolation Test and 5-min Human Approach Test. On day 15, the behavior test piglets were paired by sex and treatment (for example, a male Medium piglet paired with another male Medium piglet from a different crate) and each pair was subjected to a 10-min Social Challenge Test and immediately blood sampled. Piglet cortisol and serotonin did not differ among treatments (P > 0.10). There were no differences (P > 0.10) for number born (12.7 ± 0.4), born alive (11.7 ± 0.4), or mortality (1.1 ± 0.2). Behavior during nursing bouts was similar, with no treatment differences in number of disputes or displacements, and similar bout lengths among treatments (199.5 ± 4.6 s, P > 0.10). No differences were detected for any of the variables for Isolation or the Human Approach Tests (P > 0.10). During the Social Challenge Test, High piglets had more contacts approaching the head of the companion piglet than did either Medium or Control piglets (14.3 ± 1.1, 10.7 ± 1.1, and 9.69 ± 0.8, respectively, P < 0.02). Total number of aggressive interactions during the test tended to be greater for Medium piglets compared to High piglets (9.3 ± 1.5 vs 5.1 ± 0.9, P < 0.07). Time budget data of the litter indicate that piglets from all three treatments spent equal amounts of time active and inactive (P > 0.10). Aggression was low with 0.3 ± 0.04% of piglets displaying aggressive behavior. Feeding high concentrations of tryptophan for a short duration early in gestation does not have a negative impact on sows' subsequent offspring. [ABSTRACT FROM AUTHOR]

Previous research demonstrates that supplementing 0.20% l -glutamine (GLN) in the diets of newly weaned and transported pigs improves growth rate to a similar extent as providing dietary antibiotics (AB). However, research comparing the effects of GLN vs. AB on intestinal physiology and the microbiome is limited. Therefore, the study objective was to compare the effects of supplementing nursery diets with GLN, AB, or no dietary antibiotics (NA) on intestinal physiology and the microbiome of pigs in a production environment following weaning and transport. Mixed-sex piglets (N = 480; 5.62 ± 0.06 kg body weight [ BW ]) were weaned (18.4 ± 0.2 d of age) and transported for 12 h in central Indiana, for two replicates, during the summer of 2016 and the spring of 2017. Pens were blocked by BW and allotted to one of the three dietary treatments (n = 10 pens/dietary treatment/replicate [8 pigs/pen]): AB (chlortetracycline [441 ppm] + tiamulin [38.6 ppm]), GLN (0.20% as-fed), or NA fed for 14 d. From day 14 to 34, pigs were fed common AB-free diets in two phases. On day 33, villus height:crypt depth tended to be increased (P = 0.07; 7.0%) in GLN and AB pigs vs. NA pigs. On day 33, glucagon-like peptide 2 (GLP-2) mRNA abundance was decreased (P = 0.01; 50.3%) in GLN and NA pigs vs. AB pigs. Crypt depth was increased overall on day 33 (P = 0.01; 16.2%) during the spring replicate compared with the summer replicate. Villus height:crypt depth was reduced (P = 0.01; 9.6%) during the spring replicate compared with the summer replicate on day 33. On day 13, tumor necrosis factor-alpha and occludin mRNA abundance was increased (P ≤ 0.04; 45.9% and 106.5%, respectively) and zonula occludens-1 mRNA abundance tended to be greater (P = 0.10; 19.2%) in the spring replicate compared with the summer replicate. In addition, AB pigs had increased (P = 0.01; 101.3%) GLP-2 mRNA abundance compared with GLN and NA pigs. Microbiome analysis indicated that on day 13, dietary treatment altered the microbiota community structure (P = 0.03). Specifically, the AB pigs tended to be distinct from both the NA and GLN pigs (P = 0.08), and Lactobacillus was increased nearly 2-fold in AB compared with NA pigs (q = 0.04) and GLN pigs (q = 0.22). In conclusion, GLN supplementation tended to improve some morphological markers of intestinal health similarly to AB pigs, while the microbiome composition in GLN pigs was more similar to NA pigs than AB pigs. [ABSTRACT FROM AUTHOR]

Pre-weaned piglets prefer higher temperatures than lactating sows. To address this, swine producers use heating lamps or pads in combination with maintaining farrowing room macroenvironment temperatures at the upper end of the thermoneutral zone (24-25℃) of the lactating sow. This may inadvertently cause thermal stress for lactating sows resulting in decreased feed consumption, and ultimately, reduced milk production and litter growth. Therefore, the objective was to determine the sow level macroenvironment temperature that would improve both sow and litter productivity. In two repetitions, 37 lactating sows (parity = 3.1 ± 1.7) and their litters (d 1 litter size = 12.0 ± 1.0) were housed within 1 of 3 farrowing rooms set to different macroenvironment temperatures: LOW (n = 12 sows and litters; 15.0 ± 0.4℃), MID (n = 13 sows and litters; 19.3 ± 0.2℃), and HIGH (n = 12 sows and litters; 24.6 ± 0.8℃). Lactating sows and their litters were on trial from the onset of farrowing until weaning (19.8 ± 0.9 d of age). Individual farrowing crate temperatures were recorded throughout the trial in 0.14 s intervals using thermocouples mounted at sow head height. Piglets were provided a 0.34 x 1.52 m heating pad with a verified steady state of 35.0℃. Sow body weight (BW) was recorded at 24 h post-farrowing and at weaning, and piglet weights were measured on d 1, 7, 14 and at weaning to determine average daily BW gain (ADG). Liveborn piglet mortality was recorded throughout the trial. Sow feed intake (FI) was recorded daily from d 1 post-farrowing until weaning. The sow was considered the experimental unit for sow level measures and the litter was considered the experimental unit for piglet measures. Data were analyzed using PROC GLIMMIX in SAS 9.4 and using GLM in JMP 15. Regression models were used to determine the optimal macroenvironment temperature to improve sow FI and piglet ADG. Sow FI was greater (P < 0.01) for LOW (7.91 ± 0.38 kg) and MID (7.32 ± 0.37 kg) versus HIGH (6.37 ± 0.39 kg) sows, but similar for LOW and MID sows. Piglet ADG was greater from d 1 to weaning (P < 0.01) for LOW (0.25 ± 0.01 kg) and MID (0.26 ± 0.01 kg) versus HIGH (0.21 ± 0.01 kg) piglets, but similar for LOW and MID piglets. Pre-weaning mortality was similar (P = 0.32; 15.6%) among all treatments. A quadratic effect was observed (P < 0.01) where sow FI was maximized at a macroenvironment temperature of 17.2℃. A cubic effect was observed (P < 0.01) where piglet ADG was greatest at 17.0℃. In summary, these data have implications towards housing lactating sows and their litters at lower macroenvironment temperatures to improve growth performance. [ABSTRACT FROM AUTHOR]

Essential oils are increasingly being used in the swine industry as an alternative to antibiotics. New areas of research indicate that essential oils may alter milk composition and production in lactating sows with the potential of increasing resistance of piglets to harmful bacteria. The purpose of this preliminary study was to determine the effects of Agolin-Pig, a product containing micro-encapsulated essential oils, on milk composition and production. The treatment group (n = 13) consisted of sows fed 200 ppm of Agolin-Pig mixed in an in-house premix combined with a standard lactation diet (NRC, 2012). The control group (n = 13) consisted of sows fed the same diet without added essential oils. All sows were started on their corresponding diets 14 days before their calculated farrowing date. Sows were induced on day 114 of gestation if they had not already farrowed. Colostrum was collected within 2 hours of the onset of farrowing from all teats and mixed to form a 50 to 100 mL homogenate, representative sample. On days 3 and 14 of lactation, sows were administered 0.5 mL of oxytocin via their ear vein to induce milk ejection. Three teats were selected along the midline of the sow and milked completely into 3 separate, corresponding 50 mL conical tubes. All milk samples were stored at -20°C until analysis. Protein composition of the three teats was averaged by sow before statistical analysis. Weigh-suckle-weigh (WSW), an indirect measure of milk production, was performed on d 10 to 12 of lactation by removing piglets from the sow for 45 minutes before recording a litter weight. The piglets were then returned to the sow to nurse for 30 minutes and weighed again. Colostrum and milk samples were analyzed for protein composition via a Bradford assay using a bovine serum albumin (BSA) standard. The PROC Mixed procedure of SAS v9.4 (Cary, North Carolina) was used to evaluate the effects of Agolin-Pig on the protein composition of milk at day 0, day 3, and day 14 of lactation along with WSW data. Treatment and parity (P1 vs P = 2) were main effects with day of collection as a repeated measure and the random effect of sow. There was a significant decrease for both groups in protein composition from day 0 to 3 (P = 0.0001) and day 0 to 14 (P < 0.0001) with no significant change between day 3 to 14, which is expected as colostrum transitions into whole milk. There were no significant differences between treatment groups in protein milk composition throughout lactation. WSW data showed no significant differences between treatments in milk production. While no significant differences were observed in milk composition and production, future analysis will show the effects of Agolin-Pig on milk fat and immunoglobulin composition during lactation. [ABSTRACT FROM AUTHOR]

Grow-finish pigs (n = 192) were used in a 28-day experiment to determine if adding oregano essential oil to the water would mitigate the detrimental effects of heat stress. Pigs (initial BW = 51.9 kg) were blocked by sex and BW and randomly allotted to a 2x2 factorial arrangement of water treatment (oregano essential oil vs. control) and environment (heat stress vs. thermoneutral). There were 6 pigs/pen (4 barrows and 2 gilts/pen) and 8 pens/treatment. Pigs were given 15 d to acclimate to pens and water treatment under thermoneutral conditions (21.1℃). Following acclimation, one-half the pigs were subjected to a 3-day diurnal heat stress (12 h at 33.3℃ and 12 h at 26.7℃). On d 22 of the experiment (3 d post-heat stress), one barrow/pen was euthanized; jejunal mucosa scrapings were collected, flash frozen in liquid nitrogen, and stored at -80℃ for subsequent RNA isolation and cDNA creation. Gene expression of CDKN1A, TP53, GPX1, SOD1, SLC2A2, SLC2A5, and MUC2 were determined by qPCR using SYBR green, normalized using housekeeper genes and expressed relative to the control treatment using the 2-ΔΔCT method. Data were analyzed as RCB using GLM procedure in SAS 9.4 with fixed effects of water treatment, environment, and the interaction. Heat-stressed pigs had reduced ADG (P < 0.003) and Gain:Feed (P < 0.008) during the 3d heat stress compared with pigs housed in thermoneutral conditions. Post-heat stress, ADG (P < 0.001) and Gain:Feed (P < 0.001) increased in previously heat-stressed pigs compared with thermoneutral pigs. There was no effect of oregano supplementation before or during heat stress. However, in the post-heat stress period, ADG was increased (P < 0.05) by oregano supplementation in the water for both heat stressed and non-heat stressed pigs. Oregano supplementation did not affect ADFI before heat stress. An interaction (P < 0.005) of heat stress and water treatment was observed for ADFI during heat stress and after heat stress. During heat stress, oregano supplementation resulted in reduced feed intake. Post-heat stress ADFI was lowest for non-heat stressed pigs with no oregano supplementation. Oregano essential oil increased water consumption before (P < 0.02), during (P < 0.03), and after heat stress (P < 0.02), with an overall increase in water consumption of 35% compared with control pigs (P < 0.04). No interactions of heat stress and oregano supplementation were observed for gene expression. SLC2A2 expression tended (P < 0.08) to be downregulated when oregano essential oils were added to the water and upregulated following an acute heat stress. SLC2A5 expression was increased in pigs given oregano compared with control pigs (P < 0.02), but was unaffected by heat stress. Water delivered oregano essential oil increased water intake and had a slight effect on intestinal gene expression but did not mitigate the effects of a short-term heat stress. [ABSTRACT FROM AUTHOR]

This experiment was conducted at Purdue University swine research and education center. The objective of this study was to determine if the inclusion of fiber (8.48% vs 14.85% NDF) in the diet of boars influenced semen characteristics, concentration, motility, and morphology. Twenty-seven boars from two age groups (7 months and 18 months) and two genetic lines (maternal and terminal) were utilized in this study over a 12-week period with one week before the study data serving as a baseline for statistical analysis. Boars were blocked by age and breed and randomly allotted to receive 2.72 kg/d of cornsoybean meal diet (CON, n=13) formulated to meet NRC (2012) requirements or an isocaloric (estimated ME = 3,285 Kcal/kg) corn-soybean meal diet with 14.3% soyhulls and supplemental choice white grease (4.65%) inclusions (FIBER, n = 14). Semen was collected once per week per boar using the gloved hand method. While at the farm 3 mL of semen was mixed with 27 mL of extender (Androhep Plus, Minitube) to make a 1:10 dilution. The semen was then driven approximately 19 km to Purdue University where concentration (Nucleocounter SP-100), motility (Computer Assisted Sperm Analysis, CASA, CEROS II), and sperm cell morphology were analyzed. Statistical analyses were conducted using PROC MIXED in SAS 9.4 (Cary, NC), with the main effects of diet, age, and breed with week as a repeated measure. Significance was determined at P < 0.05 and a trend was observed at 0.05 < P = 0.10. Fiber in the diet did not affect any semen characteristics throughout the study. However, the percent morphologically normal sperm was greater in young boars compared with the older boars (80.6% vs 74.1%, P = 0.0143) resulting from fewer head/tail abnormalities (1.8% vs 2.4%, P = 0.0249) and distal droplets (7.2% vs 9.6%, P = 0.0508). Maternal boars had fewer proximal droplets (6.9% vs 9.9%, P = 0.0325) and tended to have fewer head/tail abnormalities (1.8% vs 2.3%, P = 0.0800). Terminal boars tended to have fewer distal midpiece reflex abnormalities than maternal boars (3.3% vs 4.8%, P = 0.0948). Overall feeding boars a high fiber diet had no effect on semen characteristics; however there were effects of age and breed of boar on semen characteristics. Future research is needed with varying levels and types of fiber inclusion in the diet along with differing ages and breeds of boars to better understand how fiber may impact semen characteristics. [ABSTRACT FROM AUTHOR]

Essential oils are being introduced in swine diets as an alternative to antibiotic usage. The objectives of this preliminary study were to determine the effects of Agolin-Pig, a combination of micro-encapsulated essential oils, on sow feed intake, sow health and body condition, piglet growth performance, and future evaluation of microbiota structure. The experimental design consisted of 26 sows randomly allotted to either control (n = 13) or treatment groups (n = 13). Both groups consumed diets that met NRC standards (NRC, 2012), with the treatment group receiving 200 ppm of Agolin-Pig in a premix made in-house for 2 weeks pre-farrowing, and throughout farrowing and lactation. Sow average daily feed intake (ADFI) and piglet average daily gain (ADG) were recorded and calculated to monitor effects of Agolin-Pig. Sow body weights, body condition scores (BCS), and Knauer Sow Caliper measurements were collected at the start of the trial, 1-week post-farrowing, and at weaning to determine the change in sow body condition during lactation. All data were analyzed using PROC Mixed procedure in SAS 9.4 (Cary, North Carolina) with treatment, week, and parity (P1 vs P=2) as main effects, repeated measures of sows and piglets, and number nursed used as a covariate where appropriate. All sows significantly increased ADFI (P = 0.0001) between weeks 1, 2, and 3 as expected during lactation, regardless of treatment. There was no significant impact of Agolin-Pig treatment on sow ADFI and piglet ADG. Piglets significantly increased ADG each week for 3 weeks (P ± 0.0001). During lactation, Agolin-Pig significantly reduced the loss of body condition as measured by the caliper (P = 0.028). Calipers are proven to detect changes in body condition more accurately than visual, subjective BCS, indicating that the caliper identifies a reduction in body condition that may have been missed by BCS or weight change altered by lactation. The gilts in the control group tended to show a reduction in BCS compared with sows in the control group (P = 0.074). In comparison, there was no significant difference in BCS between gilts and sows in the Agolin-Pig treated group suggesting that Agolin-Pig is effective at reducing the loss of BCS in gilts during lactation. Current analysis of fecal microbiota and milk composition may give greater insight on the effects of Agolin-Pig on sows. Overall, Agolin-Pig was effective at reducing the loss of body condition during lactation, as measured by the caliper. Additionally, Agolin-Pig tended to reduce the difference in loss of body condition of gilts compared with the control group. The findings of this pilot study warrant future studies with greater sample size to determine the efficacy of Agolin-Pig on reducing body condition loss and impacting other performance measures during lactation in sows. [ABSTRACT FROM AUTHOR]

Larger litter sizes in swine production systems and potential milk limitations for piglets have given new opportunities to incorporating creep feeding as a management strategy. Sows (n = 51) and their litters were used to test the effects of creep feed and nursery diets on piglet lactation and nursery growth performance. Ten days before weaning, litters were assigned to one of three creep feeding treatments: 1) Control diet: 2) Control+Flavoring (50 ppm Allium XL, Pancosma): 3) No creep feed. Creep feed was delivered twice/day via one bowl creep feeder/litter. Sows and litters (17/treatment) were allotted to equalize parity (average = 2.8), genetics, number of piglets (average = 10.9), and weaning age (average = 19.1). Litter weights and creep feed intake were recorded on d 0, 4, and 10 (weaning). Post-weaning pigs were allotted to Control or Flavoring (50 ppm Allium XL) diets in a 3x2 factorial arrangement. During nursery there were 10 pens/treatment with 7 or 8 pigs/pen, blocked by initial BW within creep treatment. Nursery treatments were fed in phase 1 (1.13 kg/pig) and 2 (2.27 kg/pig) diets followed by common phase 3 (6.8 kg/pig) and 4 (ad lib to d 35) diets. Weekly pig BW and feed intake were used to calculate nursery pen ADG, ADFI, and G:F. Data were analyzed using GLM procedure of SAS 9.4. Creep feeding treatment during lactation did not affect litter or piglet BW gain pre-weaning. Flavoring did not affect total creep feed intake (control = 114 g/pig vs. flavoring = 110 g/pig). Providing creep feed during lactation increased ADFI on days 2, 3, and 4 post-weaning (P < 0.013). Pigs fed either creep feed increased week 1 nursery ADFI (8.8%; P< 0.02), numerically increased ADG (5.2%), and greater d ay7 BW (P = 0.055). Week 3 ADG (P < 0.05) and ADFI (P < 0.05) were greater for the no creep pigs than pigs fed the control creep feed, flavored creep feed pigs were intermediate. For week 4, ADG, and week 4 and 5 ADFI, pigs previously fed control creep had greater growth performance than pigs previously fed flavored creep with pigs fed no creep being intermediate (P< 0.05). Overall (days 0-35), pigs fed control creep had greater ADG (P< 0.05) and ADFI (P < 0.05) than flavored creep fed pigs with no creep pigs being intermediate in ADG and greater ADFI than flavored creep fed pigs. Final BW (P < 0.05) was greater for control creep fed pigs than flavored creep fed pigs with no creep pigs being intermediate (23.5 kg, 23.0 kg, 23.3 kg, respectively). Nursery dietary flavoring treatment did not affect post-weaning ADG, ADFI, or G:F at any point during the study (P > 0.12). Lactation creep feeding significantly increases ADFI and ADG during the first week post-weaning, which could improve post-weaning performance and reduce the rate of pigs failing to thrive post-weaning. [ABSTRACT FROM AUTHOR]

Changes in weaning age and diet complexity can impact pig growth post-weaning. Weanling gilts and barrows [n = 432 Topigs Duroc X (US York X Landrace)] from the same farrowing group were utilized for a 30 or 35 day growth trial to compare early (EW) versus late weaning (LW) and high or low levels of specialty proteins in nursery diets. Pigs (n = 216) were EW (18 or 19 d, average Age = 18.4 days, average BW = 5.96 kg) and 216 pigs were LW 5 days later (24, 25 or 26 d, average Age = 24.6 d, average BW = 7.50 kg). At weaning pigs were blocked by weaning age, BW, sex and litter, and randomly allotted to 48 pens with 9 pigs/pen. Pigs at each weaning age were fed a High Complexity (HC) or a Low Complexity (LC) diet. The trial was conducted as a 2X2 factorial design: 1) EW+HC; 2) EW+LC; 3) LW+HC; 4) LW+LC. Pigs and feeders were weighed on d 0, 7, 14, 21, 28, and 35 for EW, and on d 0, 7, 14, 21, 28, and 30 for LW. The LW pigs had improved ADG and ADFI from days 0-7, 7-14, 14-21, and the final week of the nursery (P < 0.05). There were no differences in ADG and ADFI from days 21-28 (P > 0.05) between weaning ages. G:F decreased for LW pigs from days 21-28 (P < 0.01) compared with EW pigs; however, for the overall nursery trial LW pigs had increased G:F (P = 0.01). The LW pigs had increased overall nursery ADG vs EW pigs (452.5 g/d vs 400 g/d; P < 0.01). End of nursery BW was greater for LW vs EW pigs (21.70 kg vs 20.74 kg; P = 0.001). When comparing pigs of similar BW at weaning, LW pigs were lighter at end of nursery (20.13 kg vs. 21.66 kg; P < 0.01) but were more feed efficient (P < 0.01) than EW pigs. There was a BW block by weaning age interaction, EW heavy pigs had less ADG compared with LW heavy pigs; however, when comparing light weight pigs, the EW light pigs had improved ADG compared with LW light weight pigs (P = 0.017). The EW pigs were lighter at market (approximately day 146 of age) compared with LW pigs (120.78 vs 124.16 kg; P = 0.014). An interaction between weaning age, nursery diet, and sex (P < 0.07) was observed for market weight, EW barrows fed LC diets were lighter at market (120.77 kg) than EW barrows fed HC diets (126.54 kg). Whereas for LW barrows fed LC had greater market weights (130.04 kg) than LW barrows fed HC diets (127.59 kg). A similar, but nonsignificant pattern was observed for gilts. Weaning age and feeding pigs the correct diet for their age in the nursery can have a lifelong effect on the pig. [ABSTRACT FROM AUTHOR]

Weaning pigs is often concurrent with transportation-induced stress which can impair growth performance, feed efficiency, and immune resilience, contributing to reduced welfare and increased production losses. The objective of this experiment was to mitigate the adverse effects of early life transport stress on subsequent immune challenges by providing supplemental tryptophan (Trp) during the nursery period. A total of 384 pigs were transported for 8 h at weaning and used in a randomized complete block design with a 2x2 factorial arrangement of immune challenge (Vaccine challenged vs. Unvaccinated) and dietary treatment (Supplemented Trp vs. Non-supplemented Trp). Pigs were fed standard nursery diets, in four phases, over 35-d with pigs receiving 1X or 2X the NRC (2012) recommended Trp concentration. There were 12 pens/treatment with 8 pigs/pen (5 barrows and 3 gilts). Individual BW and pen feed intakes were determined weekly. One-half the pigs on each dietary treatment were subjected to a 3-wk vaccine challenge consisting of Mycoplasma (Respisure-One), Influenza (Flusure XP), and Ileitis (Porcilis) vaccines administered in week 2, 3, and 4 post-weaning, respectively. Data were analyzed as a RCB using GLM procedure in SAS 9.4 with fixed effects of dietary treatment and immune challenge, the interactions, and random effect of replicate. Beginning on d 7 and continuing throughout the nursery period, pigs fed diets containing 2X NRC (2012) Trp were heavier (P < 0.05) than pigs fed diets containing 1X Trp. Heavier BW were the result of an overall improvement in ADG for 2X Trp fed pigs (P < 0.006). There was a tendency (P < 0.08) for an interaction of dietary Trp concentration and vaccine challenge on overall ADFI, with added Trp increasing ADFI in pigs not undergoing a vaccine challenge but having no effect in vaccine challenged pigs. During week 4 post-weaning, G:F was less (P < 0.04) for vaccinated pigs compared with unvaccinated pigs. An interaction between dietary Trp and immune challenge was observed in week 5 postchallenge for G:F (P=0.0372) with G:F being reduced in vaccinated pigs fed 1X Trp, but not in vaccinated pigs fed 2X Trp; indicating that supplemental Trp may have added value in immune challenged pigs. Overall, increasing dietary Trp in nursery diets to 2X the NRC (2012) recommendation improves ADG in nursery pigs and maintains feed efficiency during a vaccine-induced immune challenge. [ABSTRACT FROM AUTHOR]

The effects of in utero heat stress (IUHS) range from decreased growth performance to altered behavior, but the long-term impact of IUHS on postnatal innate immune function in pigs is unknown. Therefore, the study objective was to determine the effects of early gestation IUHS on the immune, metabolic, and stress response of pigs subjected to an 8 hr lipopolysaccharide (LPS) challenge during postnatal life. Twenty-four pregnant gilts were exposed to thermoneutral (TN ; n = 12; 17.5 ± 2.1 °C) or heat stress (HS ; n = 12; cyclic 26 to 36 °C) conditions from days 6 to 59 of gestation, and then TN conditions (20.9 ± 2.3 °C) from day 60 of gestation to farrowing. At 12 wk of age, 16 IUHS and 16 in utero thermoneutral (IUTN) pigs were selected, balanced by sex and given an intravenous injection of LPS (2 µg/kg BW mixed with sterile saline [ SAL ] and injected at 2 µL/kg BW) or SAL (2 µL/kg BW). Body temperature was monitored every 30 min, and blood was obtained at 0, 1, 2, 3, 4, 6, and 8 hr following the LPS challenge. Blood samples were analyzed for glucose, insulin, non-esterified fatty acids (NEFA), cortisol, and cytokine concentrations. In addition, white blood cell counts were determined at 0 and 4 hr. Hour 0 data were used as covariates. Body temperature was increased (P < 0.01) in LPS (40.88 ± 0.08 °C) vs. SAL (39.83 ± 0.08 °C) pigs. Eosinophils tended to be decreased overall (P = 0.09; 43.9%) in IUHS vs. IUTN pigs. Glucose concentrations were reduced overall (P = 0.05; 5.9%) in IUHS vs. IUTN pigs. The NEFA concentrations tended to be greater (P = 0.07; 143.4%) in IUHS-LPS pigs compared with all other treatments, and IUTN-LPS pigs tended to have greater (127.4%) circulating NEFA concentrations compared with IUTN-SAL and IUHS-SAL pigs. Cortisol was increased (P = 0.04) in IUHS-LPS compared with IUTN-LPS pigs at 3 hr (21.5%) and 4 hr (64.3%). At 1 hr, tumor necrosis factor α was increased (P = 0.01; 115.1%) in IUHS-LPS compared with IUTN-LPS pigs. Overall, interleukin-1β (IL-1β) and interleukin-6 (IL-6) were greater (P < 0.04; 281.3% and 297.8%, respectively) in IUHS-LPS pigs compared with all other treatments, and IUTN-LPS pigs had increased IL-1β and IL-6 concentrations compared with IUTN-SAL and IUHS-SAL pigs. In summary, IUHS altered the postnatal cytokine, metabolic, and physiological stress response of pigs during postnatal life, which may have negative implications toward the innate immune response of IUHS pigs to pathogens. [ABSTRACT FROM AUTHOR]

Dietary antibiotic use has been limited in swine production due to concerns regarding antibiotic resistance. However, this may negatively impact the health, productivity, and welfare of pigs. Therefore, the study objective was to determine if combining dietary synbiotics and 0.20% L-glutamine would improve pig growth performance and intestinal health following weaning and transport when compared with traditionally used dietary antibiotics. Because previous research indicates that L-glutamine improves swine growth performance and synbiotics reduce enterogenic bacteria, it was hypothesized that supplementing diets with 0.20% L-glutamine (GLN) and synbiotics (SYN; 3 strains of Lactobacillus [1.2 × 10^9 cfu/g of strain/pig/d] + β-glucan [0.01 g/pig/d] + fructooligosaccharide [0.01 g/pig/d]) would have an additive effect and improve pig performance and intestinal health over that of dietary antibiotics. Mixed-sex pigs (N = 226; 5.86 ± 0.11 kg body weight [BW]) were weaned (19.4 ± 0.2 d of age) and transported for 12 h in central Indiana. Pigs were blocked by BW and allotted to one of two dietary treatments (5 to 6 pigs per pen): antibiotics (positive control [PC]; chlortetracycline [441 ppm] + tiamulin [38.5 ppm]), no antibiotics (negative control [NC]), GLN, SYN, or the NC diet with both the GLN and SYN additives (GLN + SYN) fed for 14 d. From day 14 post-weaning to the end of the grow-finish period, all pigs were provided common antibiotic-free diets. Data were analyzed using PROC GLIMMIX and PROC MIXED in SAS 9.4. Overall, haptoglobin was greater (P = 0.03; 216%) in NC pigs compared with PC pigs. On day 13, GLN and PC pigs tended to have reduced (P = 0.07; 75.2% and 67.3%, respectively) haptoglobin compared with NC pigs. On day 34, the jejunal goblet cell count per villi and per millimeter tended to be greater (P < 0.08; 71.4% and 62.9%, respectively) in SYN pigs compared with all other dietary treatments. Overall, jejunal mucosa tumor necrosis factor-alpha (TNFα) gene expression tended to be greater (P = 0.09; 40.0%) in NC pigs compared with PC pigs on day 34. On day 34, jejunal mucosa TNFα gene expression tended to be greater (P = 0.09; 33.3%, 41.2%, and 60.0%, respectively) in GLN pigs compared with SYN, GLN + SYN, and PC pigs. Although it was determined that some metrics of pig health were improved by the addition of GLN and SYN (i.e., haptoglobin and goblet cell count), overall, there were very few differences detected between dietary treatments and this may be related to the stress load incurred by the pigs. [ABSTRACT FROM AUTHOR]

In utero heat stress (IUHS) increases the energy requirements of pigs during postnatal life, and this may compound weaning and transport stress. The study objective was to evaluate and mitigate the negative effects of IUHS following weaning and transport through the provision of a nutrient-dense (ND) nursery diet formulated to meet the greater energy requirements of IUHS pigs during the first 14 d post weaning and transport. Twenty-four pregnant gilts were exposed to thermoneutral (TN; n = 12; 17.5 ± 2.1 °C) or heat stress (HS; n = 12; cycling 26 to 36 °C) conditions for the first half of gestation (day 6 to 59) and then TN conditions (20.9 ± 2.3 °C) until farrowing. Nine TN gilts and 12 HS gilts produced litters. At weaning (16.2 ± 0.4 d), mixed sex piglets (N = 160; 4.78 ± 0.15 kg body weight [BW]) were transported (loading + transport + unloading) for 11 h 40 min. Following transport, piglets were blocked into pens (n = 4 pigs/pen) by in utero and dietary treatments: in utero thermoneutral (IUTN) + control (C) diet (n = 10 pens), IUTN + ND (n = 10 pens), IUHS + C (n = 10 pens), and IUHS + ND (n = 10 pens). Treatment diets were fed from day 1 to 14 post weaning and transport (period 1), and the C diet was fed to all pigs from day 14 to 35 post weaning and transport (period 2). Production measures were taken in 7 d intervals to calculate average daily gain (ADG), average daily feed intake (ADFI), average daily net energy intake (ADEI), gain: feed, and gain: net energy intake. Blood samples were collected prior to transport, following transport, and on days 2, 7, 14, 28, and 35 post weaning and transport to analyze cortisol, glucose, insulin, and nonesterified fatty acids. Behavior was assessed through video-recording on days 3, 5, 8, 11, and 13 post weaning and transport. In period 1, ADG was reduced (P = 0.04; 20.0 g/d) in IUHS vs. IUTN pigs. Pigs fed ND diets had reduced ADFI (P = 0.02; 9.3%) compared with C diet-fed pigs during period 1, which resulted in similar ADEI (P = 0.23; 1,115 ± 35 kcal/d). During transport, cortisol was decreased (P = 0.03; 25.8%) in IUHS vs. IUTN pigs. On day 2, glucose was decreased (P = 0.01; 13.8%) in IUHS vs. IUTN pigs. No in utero treatment-related behavior differences were observed but lying was reduced (P = 0.03; 6.5%) and standing was increased (P = 0.04; 14.1%) in ND vs. C pigs overall. In summary, IUHS reduced growth performance in pigs following weaning and transport, and providing an ND diet did not rescue the lost performance. [ABSTRACT FROM AUTHOR]

Supplementing nursery diets with 0.20% L-glutamine (GLN) may provide similar growth and health benefits as dietary antibiotics, but it was unknown if greater inclusion levels may provide additional benefits. Therefore, the study objective was to evaluate the impact of replacing dietary antibiotics with increasing GLN levels on growth performance, therapeutic antibiotic treatment rates, welfare measures, and production costs in pigs after weaning and transport. We hypothesized that withholding dietary antibiotics may negatively impact performance and increase therapeutic treatment rate, and that diet supplementation with 0.20% to 1.00% GLN may incrementally improve productivity and reduce therapeutic antibiotic treatment rates compared with dietary antibiotics. Mixed sex pigs (N = 308; 5.64 ± 0.06 kg body weight [BW]) were weaned (19.1 ± 0.2 d of age) and transported in central Indiana in 2017. Pigs were blocked by BW and allotted to one of seven dietary treatments (n = 8 pens/dietary treatment): dietary antibiotics (positive control [PC]; chlortetracycline [441 mg/kg] + tiamulin [38.6 mg/kg]), no antibiotics or added GLN (negative control [NC]), 0.20% GLN, 0.40% GLN, 0.60% GLN, 0.80% GLN, or 1.00% GLN fed for 14 d. From d 14 to 35, pigs were provided nonantibiotic common diets in two phases. Overall, average daily gain (ADG) was reduced (P = 0.01; 17.7%) from d 0 to 14 in NC, 0.20% GLN, 0.60% GLN, 0.80% GLN, and 1.00% GLN pigs compared with PC pigs, but no ADG differences were detected between 0.40% GLN pigs and PC pigs. Increasing GLN in the diet tended to increase ADG (linear; P = 0.10). Overall, d 35 BW was greater (P = 0.01) in 0.80% GLN and PC pigs compared with NC, 0.20% GLN, and 0.60% GLN pigs, and was greater for 0.40% GLN and 1.00% GLN pigs vs. 0.20% GLN pigs. However, no d 35 BW differences were detected (P > 0.05) between PC, 0.40% GLN, 0.80% GLN, and 1.00% GLN pigs. Increasing GLN in the diet tended to increase (linear; P = 0.08) d 35 BW. Overall, income over feed and therapeutic injectable antibiotics cost (IOFAC) for enteric and unthrifty challenges were greater (P = 0.02) in 0.80% GLN pigs compared with NC, 0.20% GLN, and 0.60% GLN pigs, but no IOFACs for enteric and unthrifty challenges differences were detected between 0.80% GLN pigs and 0.40% GLN, 1.00% GLN, and PC pigs. In conclusion, GLN supplemented pigs had improved performance after weaning and transport compared with the NC pigs with 0.40% GLN being the most effective level. [ABSTRACT FROM AUTHOR]

Antibiotic use has been limited in U.S. swine production. Therefore, the objective was to determine whether supplementing l -glutamine at cost-effective levels can replace dietary antibiotics to improve piglet welfare and productivity following weaning and transport. Based on previous research, we hypothesized that withholding dietary antibiotics would negatively affect pigs while diet supplementation with 0.20% l -glutamine (GLN) would have similar effects on pig performance and health as antibiotics. Mixed sex piglets (N = 480; 5.62 ± 0.06 kg BW) were weaned (18.4 ± 0.2 d of age) and transported for 12 h in central Indiana, for 2 replicates, during the summer of 2016 and the spring of 2017. Pigs were blocked by BW and allotted to 1 of 3 dietary treatments (n = 10 pens/dietary treatment/replicate [8 pigs/pen]); antibiotics (A ; chlortetracycline [441 ppm] + tiamulin [38.6 ppm]), no antibiotics (NA), or GLN fed for 14 d. On days 15 to 34, pigs were provided common antibiotic-free diets in 2 phases. Data were analyzed using PROC MIXED in SAS 9.4. Day 14 BW and days 0 to 14 ADG were greater (P = 0.01) for A (5.6% and 18.5%, respectively) and GLN pigs (3.8% and 11.4%, respectively) compared with NA pigs, with no differences between A and GLN pigs. Days 0 to 14 ADFI increased for A (P < 0.04; 9.3%) compared with NA pigs; however, no differences were detected when comparing GLN with A and NA pigs. Once dietary treatments ceased, no differences (P > 0.05) in productivity between dietary treatments were detected. On day 13, plasma tumor necrosis factor alpha (TNF-α) was reduced (P = 0.02) in A (36.7 ± 6.9 pg/mL) and GLN pigs (40.9 ± 6.9 pg/mL) vs. NA pigs (63.2 ± 6.9 pg/mL). Aggressive behavior tended to be reduced overall (P = 0.09; 26.4%) in GLN compared with A pigs, but no differences were observed between A and GLN vs. NA pigs. Huddling, active, and eating/drinking behaviors were increased overall (P < 0.02; 179%, 37%, and 29%, respectively) in the spring replicate compared with the summer replicate. When hot carcass weight (HCW) was used as a covariate, loin depth and lean percentage were increased (P = 0.01; 4.0% and 1.1%, respectively) during the spring replicate compared with the summer replicate. In conclusion, GLN supplementation improved pig performance and health after weaning and transport similarly to A across replicates; however, the positive effects of A and GLN were diminished when dietary treatments ceased. [ABSTRACT FROM AUTHOR]

This experiment was conducted at the Purdue University swine research and education center. The objective of this study was to determine if the inclusion of fiber (8.48% vs 14.85% NDF) in the diet influenced boar body weight, backfat depth, loin depth, Knauer sow caliper measurement, flank to flank distance, and visual body condition score (BCS). Twenty-seven boars from two age groups (7 months and 18 months) and two genetic lines (maternal and terminal) were utilized over a 12-week period with 1 week before the study data serving as baseline for statistical analysis. Fecal samples were also taken and scored using the Bristol stool scoring system. Timepoints for sample collection were on weeks -1,3,7, and 11. Boars were blocked by age and breed and randomly allotted to receive 2.72 kg/d of corn-soybean meal diet (CON, n = 13) formulated to meet NRC (2012) requirements or an isocaloric (estimated ME = 3,285 Kcal/kg) cornsoybean meal diet with 14.3% soyhulls and supplemental choice white grease (4.65%) inclusions (FIBER, n = 14). Data were analyzed using PROC GLM in SAS 9.4 (Cary, North Carolina) with boar serving as the experimental unit. Weeks were analyzed individually and changes in body weight, backfat depth, loin depth, caliper measurements, flank to flank distance, and BCS were evaluated from week -1 to week 12. Significance was determined at P < 0.05 and a trend was observed at 0.05 < P = 0.10. FIBER boars gained 8.4 kg more than CON boars during the 12-week study (P = 0.0221). On weeks 3 and 11, FIBER boars tended to have increased backfat depth compared with CON boars (P < 0.0950), resulting in FIBER boars gaining 2.9 mm more backfat compared with CON (P = 0.0053) over the 12-week study. No difference in loin muscle depth was observed between treatments. On weeks 7 (3.7 vs 3.4, P = 0.0923) and 11(3.9 vs 3.5, P = 0.0615) FIBER boars tended to have greater BCS score compared with Con boars. FIBER boars on week 11 tended to have larger tape measurements (105.9 cm vs 103.9 cm, P = 0.0945) and larger caliper score (19.4 vs 17.6, P = 0.0029) than CON boars, but no difference in overall 12 week change for both measurements. FIBER boars tended to have softer stools during week 3 of the trial compared with CON boars (P = 0.0535), but the fecal scores were not different at any other week. Overall, boars fed a high fiber diet had increased body weight, backfat depth and BCS indicating an underestimation of the net energy value of soyhulls in limit fed boars. Future research is needed with varying levels and types of fiber inclusion in the diet to understand how to correctly implement fiber into the boar diet and to refine the net energy values of fibrous feedstuffs like soyhulls for boars. [ABSTRACT FROM AUTHOR]

Weaning is one of the most stressful events for piglets, due to diet change from sow milk to grainbased feed. This diet change causes turnover of the gut microbial which can allow proliferation of pathogens leading to diarrhea. Antibiotics are administered to treat or prevent post-weaning diarrhea (PWD). Increased occurrence of antimicrobial resistance has increased incentives to develop viable antibiotic alternatives. Previous studies have shown that fecal microbiota transplant (FMT) reduced diarrhea incidence and improved body weight gain in piglets. However, one barrier to FMT adoption in swine production is that FMT are generally given via oral gavage, which is difficult and labor intensive for large swine operations. For FMT to be a viable option in swine management, a simple method is needed to administer the transplant. The objective of this study was to determine the effect of three methods of FMT administration (oral, rectal and lyophilized in-feed) on growth performance, diarrhea incidence and gut microbiome. We hypothesize that FMT (in-feed and rectal) will increase colonization efficiency, improve growth performance and reduce diarrhea incidence compared with control. Weaned male piglets (n = 40) were allotted to 4 treatments at 10 piglets per treatment (no FMT - Control, Oral gavage - FMT1, rectal gavage - FMT2 and lyophilized in-feed - FMT3). Feces from 12-week-old pigs, screened for pathogens, was donor material. The study lasted nine days and FMT was for five days. Diarrhea incidences and body weight were observed for the first week. Fecal samples were collected on days 0, 2, 5 and 7. Five piglets per group were sacrificed on day 4 and the remaining on day 8. Cecal and colon contents were collected from the sacrificed piglets. DNA was extracted from fecal and digesta samples and 16S rRNA gene libraries (V4 region) were prepared for bacterial community analysis. Data were analyzed with one-way ANOVA in R. All FMT groups had significantly greater body weight on day 3 (P < 0.05) and average daily gain between day 0 - 3 (P < 0.05) compared with control but there was no difference between FMT groups. There was no effect of FMT on average daily feed intake and diarrhea incidence. Only FMT3 had greater alpha diversity (Observed features and Faith's phylogenetic diversity) on day 5 (P < 0.05) compared with control, but there was no difference in beta diversity between FMT groups. There was decreased abundance of 27 ASVs (amplicon sequence variant) and increased abundance of 30 ASVs in increased in FMT3 compared with control on day 5. In conclusion, the three FMT groups has similar colonization pattern but there was no difference in diarrhea incidence. FMT3 (lyophilized in-feed) altered overall community structure of piglets and modulated individual taxa. [ABSTRACT FROM AUTHOR]

Weaning and transport stress impairs intestinal function and reduces nutrient absorption leading to reduced growth performance and feed efficiency. The objective of this study was to eliminate or reduce short- and long-term, transport-induced reductions in feed efficiency and growth by providing supplemental Tryptophan (Trp) above NRC (2012) pre- and/or postweaning. A total of 480 pigs were blocked by sex, ancestry, and body weight (BW) and used in a RCB design with a 2x2x2 factorial arrangement of pre-weaning treatment (oral gavage of Trp at 0.35, 0.45, and 0.55 g/d (increasing every 5d) pre-weaning or control), transport (transported or not at weaning), post-weaning nursery treatment (1X or 2X NRC Trp). At farrowing pigs were blocked by sex, within litter, and randomly assigned to either a pre-wean Trp or control treatment, where Trp supplementation began on d 5 post-farrowing and stopped at weaning. Post-weaning Trp supplementation included a 3 d oral gavage of Trp and 2X Trp in the diet for the entire 42 d nursery period. At weaning, all pigs were blocked by sex, weaning weight and prewean treatment and randomly assigned to transport and post-wean treatments. Pigs were fed four nursery phases with diets containing 1X or 2X NRC recommended concentrations of Trp. There were 10 pens/trt with 6 pigs/pen (equal barrows and gilts). Individual BW were determined every 5 d pre-weaning and every 7 d post-weaning along with pen feed intakes. Data were analyzed as a RCB using GLM procedure in SAS 9.4 with fixed effects of pre-wean dietary treatment, transport treatment, and post-wean dietary treatment, the interactions, and random effect of replicate. Preweaning Trp supplementation had no effect on preweaning growth performance. A 3-way interaction of pre-wean treatment x transportation x post-wean treatment was observed for overall nursery BW (P = 0.0148) and ADG (P = 0.0175). This interaction was the result of post-weaning Trp supplementation increasing BW and ADG in all pigs, except for transported pigs that did not receive Trp pre-weaning. While there were 2-way interactions of pre-wean treatment x post-wean treatment on ADFI at week 1 (P= 0.0123) and week 6 (P = 0.0297) and a 3-way interaction of pre-weaning treatment x transportation x post-weaning treatment at week 2 (P = 0.0092), there were no consistent or overall effects on overall ADFI or G:F. Tryptophan improves transported pigs BW and ADG when supplemented pre- and post-weaning but does not benefit transported pigs when only supplemented post-weaning, suggesting transportation induced reductions in BW and ADG can be mitigated by supplementing Trp prior to and following transportation. [ABSTRACT FROM AUTHOR]

There is an ever-imminent need for career-ready graduates from collegiate agriculture programs. Generational shifts in attitudes and background experiences of agricultural students challenge educators to maintain curricular programming which establishes a successful career trajectory for students. Students studying animal science and/or agricultural economics were surveyed to understand their perception of how collegiate curricular, co-curricular, and extracurricular experiences (coursework, club participation, relevant work, international experience, advising/mentoring, college life, and professional networking) contribute to their anticipated career success. A best-worst scaling experiment was used to force respondents to make tradeoffs between the collegiate experience attributes in a manner designed to be free of scale biases. Responses were related back to additional demographical and experience/perception characteristics of respondents through various approaches. Based on their responses, students solely in a pre-veterinary Animal Science curriculum represented a particularly interesting category of students regarding their beliefs and reported experiences. Students indicated relevant work experience was overwhelmingly the most critical of the 7 factors they were asked to evaluate. Further research should investigate possible disconnects between student perceptions and reality in higher education. [ABSTRACT FROM AUTHOR]

Two trials were conducted to determine the effect of swine manure application on wheat growth and nutrient uptake. Manure was added to soil on a nitrogen (N) basis at 325 kg plant-available nitrogen (PAN) ha-1 (experiment 1) and on a phosphorus (P) basis at 50 kg P ha-1, while maintaining a rate of 325 kg PAN ha-1 through ammonium sulfate addition (experiment 2). Manure treatments increased overall wheat growth by 29% (P < 0.005) compared to the negative control (NC) and increased plant tissue N mass 48% (P < 0.001) and P mass 61% (P < 0.002) (experiment 1). Fertilizer control tended to increase (P < 0.10) wheat growth and increased total vegetative tissue N mass by 23% (P < 0.006) and potassium mass by 20% (P < 0.002) compared to manure treatments. Manure treatments increased wheat growth by 23% (P < 0.03) compared to the NC at the first harvest (experiment 2). [ABSTRACT FROM AUTHOR]

The purpose of this study was to test an educational swine curriculum geared toward fifth grade classrooms to measure the change in students' knowledge about the pork industry, pork as a nutritious protein source, and the value of byproducts derived from pork production. Objectives of this study were to evaluate overall change in students' knowledge of the pork industry and the effect of specific demographics on the change in students' knowledge following participation in an educational swine curriculum. Effectiveness of the curriculum was measured by a pretest/post-test survey of fifth grade students (n = 435), with classrooms divided into treatment and control groups. Findings indicated that participating in the educational swine curriculum increased the students' knowledge of the pork industry by 37. 4%; demographics such as 4-H experience, farm experience, or prior experience with pigs had limited effect on knowledge gained. [ABSTRACT FROM AUTHOR]

Relative predominance of each of five probiotic strains was investigated in the ileum of weaned pigs, compared with that in feces, when administered in combination at c. 5 × 109 CFU day−1 for 28 days. Probiotic was excreted at 106–109 CFU g−1 feces, while ileal survival ranged from 102 to 106 CFU g−1 digesta. In contrast to the feces, where Lactobacillus murinus DPC6002 predominated, the bacteriocin-producing Lactobacillus salivarus DPC6005 dominated over coadministered strains both in the ileum digesta and in mucosa. Probiotic administration did not alter counts of culturable fecal Lactobacillus or Enterobacteriaceae but higher ileal Enterobacteriaceae were observed in the ileal digesta of probiotic-fed pigs ( P<0.05). We observed decreased CD25 induction on T cells and monocytes ( P<0.01) and decreased CTLA-4 induction ( P<0.05) by the mitogen phytohemagglutinin on CD4 T cells from the probiotic group. Probiotic treatment also increased the proportion of CD4+ CD8+ T cells within the peripheral T-cell population and increased ileal IL-8 mRNA expression ( P<0.05). In conclusion, superior ileal survival of L. salivarius compared with the other coadministered probiotics may be due to a competitive advantage conferred by its bacteriocin. The findings also suggest that the five-strain combination may function as a probiotic, at least in part, via immunomodulation. [ABSTRACT FROM AUTHOR]

Hyperprolific sows can have a long duration of farrowing, negatively impacting piglet survival and vitality. This study's objective was to assess the effect of a diet containing 6.6% resistant starch (RS) on sow and piglet farrowing performance. We hypothesized that a diet containing RS would decrease sow farrowing duration and improve piglet survival. Forty-two sows were balanced for parity and randomly assigned to 1 of 2 treatments: standard lactation diet (n = 21) or diet containing RS (n = 21). Sows were fed their respective diets from approximately 7d prefarrowing throughout lactation. Sow blood was drawn at three time points on d106±1.0 and d113±1.0 of gestation (pre-feeding, 2 and 6 h post-feeding) and during farrowing to measure blood glucose. Piglet umbilical blood (~3/litter; beginning, middle, end of birth order) was collected at birth and analyzed for glucose, lactate, pH, PCO2, PO2, TCO2, HCO3, BE, and sO2. Piglets were visually assessed at birth to score meconium staining. Data collected included farrowing duration, piglet birthing intervals, number of stillborn piglets/litter, sow and piglet weights, piglet mortality, and sow feed and water intake. Average litter sizes post-crossfoster for control and RS were similar, 12.1±0.4 and 11.6±0.4 piglets, respectively. Litter weight gain was similar between control and RS (44.97 vs. 47.54 kg, P = 0.33), respectively. Feeding resistant starch had no effect on number of stillborn piglets or pre-weaning survival (P > 0.75). Farrowing duration (178.9±17.6 min vs 165.2±18.0 min, P = 0.59) and piglet birth intervals (16.57±1.7 min and 14.89±1.8 min, P = 0.51) did not differ between control and RS, respectively. There was difference in sow blood glucose between diets (P = 0.02), day of sample (P < 0.0001), time point (P < 0.0001), and a day by time point interaction (P < 0.0001). Feeding RS to sows prefarrowing can modify sow blood glucose, but did not result in reduced farrowing duration or improved piglet survival. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the effects of total or partial replacement of animal and fish proteins with a fortified protein blend (PROPLEX MVP) on growth performance of nursery piglets. PROPLEX MVP is a blend of two distinct fermentation biomass streams, refined soybean products, select amino acids, and a NSP enzyme complex. A total of 306 barrows and gilts [(Duroc x (Landrace x Yorkshire), avg. 22 d age and BW 6.37 ± 1.24 kg)] were used in a randomized complete block design with three dietary treatments, each with 19 replicates (pens) of 5 piglets. Dietary treatments were: 1) Control diet (CONT) with animal and fish protein sources (Blood meal, Spray-dried Plasma, and Fish meal); 2) PROPLEX MVP (MVP): CONT with MVP replacing Fish meal; 3) PROPLEX MVP 100 (MVP100): CONT with MVP replacing 100% of animal and fish proteins. Pigs were fed a budgeted amount per head of the assigned experimental diets for the first three feeding phases (Phase 1: 2.3 kg/pig; Phase 2: 4.5 kg/pig; Phase 3: 6.8 kg/pig). A common diet was provided in Phase 4 until day 42 post-weaning. Pigs fed CONT had greater ADG (P < 0.05) compared to MVP100 pigs d 0-7 post-weaning. From d 7 to 14, pigs fed MVP and MVP100 had a significantly greater ADG and G:F (P < 0.05) than pigs fed CONT diet. For the remainder of the experimental period (d 14 to 42), no differences (P > 0.10) were observed among treatments. The use of MVP100 reduced (P < 0.05) the total nursery feed cost by US $1.03/pig and feed cost/kg of weight gain by 12%. In conclusion, MVP protein blend can effectively substitute for animal and fish proteins sources without compromising overall nursery pigs' growth performance and results in significant feed cost savings. [ABSTRACT FROM AUTHOR]

The objective of this study was to determine the optimal level of Cordyceps mushroom powder inclusion in diets for nursery pigs. One-hundred sixty crossbred pigs [(Duroc × (York × Landrace)] weaned at 18.8 d of age and weighing an average of 5.94 kg were used in a 35 day, 4 phase growth trial to evaluate Cordyceps mushroom powder as potential alternative to carbadox in nursery pig diets. Pigs were allotted by weight, sex, litter, and assigned to body weight (BW) blocks. Within BW blocks, sex ratios were constant in each pen. Each pen within a BW block was randomly assigned a dietary treatment. Growth performance was analyzed as a RCB design using BW, ADG, ADFI, G:F using GLM procedure of SAS 9.4. There were 5 or 6 pigs/pen and 6 pens/treatment. Five diets were used in the study: a negative diet or a positive control (Carbadox, 55 ppm); 300 or 600 ppm mushroom powder, and a step down treatment (900, 900, 450, 300, 150 ppm mushroom powder during weeks 1 through 5, respectively). At various points of the study, pigs fed the 300 ppm and the step-down mushroom powder treatments tended to have improved (P < 0.10) growth performance compared with those fed the negative control diet. During Phase 4 of the study, pigs fed Carbadox had greater ADG (P < 0.02) and improved feed efficiency (P < 0.09) compared to pigs fed the negative control diet. However, overall data showed that there were no statistical differences among treatments (P > 0.05). In summary, pigs fed 300 ppm mushroom powder or the step-down treatment showed comparable growth performance to pigs fed Carbadox. However, future research is needed under a greater disease challenge to examine mushroom powder's full potential as an alternative to antibiotics. [ABSTRACT FROM AUTHOR]

The objective was to evaluate the independent and additive effects of Cordyceps mushroom powder and carbadox to pharmacological copper+zinc in nursery pig diets. Two hundred-ten crossbred weanling pigs [(Duroc × (York × Landrace)] avg. 19 d of age and 5.8 kg were used in a 33 day growth trial. Pigs were alloted by weight, sex, ancestry, and assigned to body weight (BW) blocks. Within BW blocks, sex ratios were constant in each pen. Pen was the experimental unit and growth performance was analyzed using BW, ADG, ADFI, and G:F. There were 7 pigs/pen and 6 pens/treatment. Treatments were: 1) a negative diet (NC); 2) positive control (PC; Carbadox, 55 ppm); 3) NC+300 ppm Cordyceps mushroom powder (NC+MP); 4) PC +300 ppm mushroom(PC+MP); 5) supplemental copper sulfate (125 ppm) and zinc oxide (3000 ppm d 0-7, 2000 ppm d 7-35), CuZn. Dietary treatments were fed in a four-phase feeding program (d0-7, d7-14, d14-21, and d21-33). There were no interactions between MP and Carbadox at any time point (P > 0.10). Pigs fed the PC, PC+MP and CuZn treatment had increased BW (P < 0.05), ADG (P < 0.05), ADFI (P < 0.10) and G:F (P < 0.05) over the NC at the end of phases 1, 2, and 3, with no main effect of MP treatment. During Phase 4, pigs fed MP, PC, and CuZn diets all had increased ADG (P < 0.05; 431, 477, 455, 505, 486 g/d, diet 1-5, respectively) and ADFI (P < 0.05) over the NC fed pigs. Overall, d0-33, PC diets and CuZn supplemented pigs had increased ADG (P < 0.05) and ADFI (P < 0.05), with pigs fed MP tending to have increased ADFI (P < 0.08) over NC fed pigs. Feeding nursery pigs pharmacological levels of Cu+Zn and carbadox have economical value to increase nursery pig performance with MP may increase pig ADFI and final BW through potentially complimentary modes of action. [ABSTRACT FROM AUTHOR]

This experiment observed the effects of replacing animal and fish proteins with a fortified protein blend (PROPLEX MVP) on growth performance, immune status, microbial metabolites, and fecal scoring of nursery pigs. A total of 244 barrows and gilts [Duroc x (Landrace x Yorkshire), avg. 19 d age and BW 5.88 ± 1.38 kg] were used in a 2 × 2 factorial design (2 dietary treatments × 2 feeding programs). The dietary treatments were: 1) Control diet (CONT) with animal and fish proteins and 2) PROPLEX MVP (MVP100): CONT with MVP replacing 100% of the animal and fish proteins. The feeding programs for each feeding phase were: high budget (H): Phase 1: 2.3 kg/pig; Phase 2: 4.5 kg/pig; Phase 3: 6.8 kg/pig and low budget (L): Phase 1: 1.1 kg/pig; Phase 2: 2.3 kg/pig; Phase 3: 3.4 kg/pig). For d 0-7, pigs fed CONT had greater ADG, ADFI, and G:F (P < 0.001) compared to MVP100. From d 7 to 14, ADG and G:F were greater (P < 0.05) for pigs fed MVP100 compared to CONT pigs and greater for pigs fed the H budget compared to the L budget (P < 0.05). Overall, d 0 to 35, there were no differences (P > 0.05) in pig final BW, overall ADG, and G:F. For week two and most of the experimental period, pigs fed CONT had greater (P < 0.05) fecal scores, more soft and watery feces, and increased diarrhea incidence compared to MVP100 pigs. The total concentration of fecal volatile fatty acids was greater (P < 0.05) in CONT compared to MVP100 fed pigs. In conclusion, the MVP protein blend can be used as a cost effective alternative to animal and fish proteins in nursery pig diets without compromising overall growth performance, immune status, and post-weaning diarrhea. [ABSTRACT FROM AUTHOR]

Dacitic tuff breccia (DTB) and poultry by-products (PBP) were added to nursery pig diets to evaluate their effects on growth performance in a 35 d experiment. A randomized complete block design based on BW and sex was used. Newly weaned pigs (N=564, 20.1 d of age, 6.18±0.006 kg initial BW) allotted to four diets (15 replicates/treatment; 9 or 10 pigs/pen): 1) Negative control (NC; no DTB or PBP), 2) NC+DTB (0.5% inclusion), 3) NC+PBP (4% chicken by-product meal + 2% feather meal - replacing fishmeal, corn, and synthetic amino acids in the NC), 4) NC+DTB+PBP. Phase 1-3 were each 7-d and Phase 4 was from d21-35. During week one there were no differences (P >0.10) among treatments. In week 2, pigs fed DTB tended to increase ADG (323 vs. 303 g/d; P=0.068) and ADFI (376 vs. 356 g/d; P=0.055) compared to pigs not fed DTB and pigs fed PBP tended to have increased ADG (P=0.093). In week 3 pigs fed PBP had reduced ADG (460 vs 483 g/d; P=0.011) and G:F (0.689 vs. 0.723; P< 0.001), and there tended to be an interaction for G:F (P=0.083) with DTB improving G:F when fed in combination with PBP but reducing G:F in the control diet. In week 4, feeding PBP decreased ADG (384 vs 415 g/d; P< 0.01) and ADFI (629 vs 666 g/d; P< 0.01) compared to pigs fed no PBP. There were no differences among treatments during week 5. For Phase 4 (d21-35) pigs fed PBP had decreased ADG (479 vs 497 g/d; P=0.041) and tended to have reduced ADFI (P=0.092) compared to pigs fed no PBP. Overall (d 0-35), there were no significant differences among treatments. In conclusion, DTB improved nursery pig growth performance early, however, pigs fed PBP had decreased ADG and feed efficiency late in the nursery period. [ABSTRACT FROM AUTHOR]

Lactating sows (N=140, York x Landrace) were used to evaluate the effects of a liquid prototype (LIQP) and dry (XPC®; Diamond V) Saccharomyces cerevisiae feed additives on sow and litter performance. Sows were fed a common gestation diet (0.55% SID-Lysine) until d112 of pregnancy and then allotted to lactation treatments: 1) Control diet (CON; 1.00% SID-Lysine), 2) CON +15 mL of LIQP from d112 to weaning (LIQ), 3) CON +0.20% of XPC from d112 to weaning (DRY), and 4) DRY +15 mL of LIQP from d112 to d7 post-farrowing (D+L). The LIQP was given once daily using an oral dose gun and XPC was included in the feed. Immunoglobulin concentrations were estimated on colostrum samples using Brix refractometer and piglet d 1 serum immunocrit ratio and plasma IgA and IgG. Daily sow water intake and daily feed intake (DFI) from d112 of gestation to d7 post-farrowing and weekly feed intake (ADFI) were recorded. There were no treatment effects on sow BW, backfat, or loin depth (P >0.05) although sows from LIQ group had numerically smaller BW reductions compared to CON sows (4.9% vs 7.2%, P=0.19). LIQ sows had greater DFI and CON lower DFI during week 1 of lactation (P=0.04) as well as ADFI for weeks 2, 3, and overall lactation period (P< 0.01) with DRY and D+L sows being intermediate. Water intake, immunoglobulins, and litter performance did not differ among treatments (P >0.05) although pigs from LIQ sows, compared to CON, had numerically increased weaning (6.14 vs 5.82kg, P >0.05) and litter weights (63.2 vs 60.1kg, P >0.05). LIQ sows had 0.7 d reduced wean-to-estrus interval (P< 0.001) and tended to have greater conception rates (P=0.07). In conclusion, LIQ supplementation of lactating sows improves feed intake, allowing sows to keep body reserves, and have better subsequent rebreeding performance. [ABSTRACT FROM AUTHOR]

A developmental Bacillus based direct-fed microbial (DFM) was added to nursery pig diets to evaluate the effect on growth and health in two 35-d randomized complete block design experiments blocked on initial BW and sex. Experiment 1 used 315 weaned pigs (20.1 d of age; 6.11 kg initial BW) allotted to one of three diets: 1) Negative Control (NC; no antibiotics but with pharmacological Zn or Cu), 2) NC+DFM 0.55x109CFU, 3) NC+DFM 1.1x109CFU. Experiment 2 used 376 weaned pigs (17.8 d of age; 5.99 kg initial BW) allotted to one of four diets, the same 3 diets from Experiment 1 plus a lower inclusion rate: 4) NC+DFM 0.275x109CFU. Both studies had 15 replicates/treatment, 6-7 pigs/pen. Pigs were budget fed phase 1-3 diets (1.13 kg/pig; 2.72 kg/pig; and 6.35 kg/pig, respectively) and ad libitum fed Phase 4 diet to d35. For Experiment 1, during week 1 pigs fed the DFM tended to linearly decrease ADFI (P = 0.086) and linearly increase G:F (P = 0.085). During day 21-35 of Experiment 1 pigs fed DFM linearly increased ADG (P = 0.035) and quadratically increased ADFI (P = 0.027). Overall for experiment 1, ADG (P = 0.114) and ADFI (P = 0.104) tended to quadratically increase and G:F linearly increased (P = 0.012) with increasing concentrations of DFM. Experiment 2; during week 1 and 3 ADFI (P = 0.084, P = 0.050, respectively) quadratically increased and G:F (P = 0.081; P = 0.022, respectively) quadratically decreased as DFM increased in the diet. During d21-35 of experiment 2, ADG (P = 0.064) quadratically increased and G:F (P = 0.012) quadratically decreased as DFM increased. Overall for Experiment 2 ADFI numerically increased (4.3%) with no increase in ADG, resulting in a quadratic decrease in G:F (P = 0.010) as the DFM increased in the diet. Summarizing these two studies, the DFM product had its greatest effect increasing ADFI while inconsistently impacting gain and efficiency. [ABSTRACT FROM AUTHOR]

Progeny from sows fed diets containing a Bacillus licheniformis direct-fed microbial (DFM), an organic acid blend of medium and short chain fatty acids (OA), DFM+OA, or a control (CON) diet from d80 of gestation until weaning were used to determine if feeding CON, DFM, OA, or DFM+OA to the dam and/or progeny post-weaning improved nursery growth performance. Weaned pigs from 47 dams (n = 384, Initial BW=6.15 kg) were blocked by initial BW and sex and allotted (6 pigs/pen, 8 pens/treatment) to one of 8 nursery treatments. Pigs from CON sows were fed a negative (NC; no antibiotics, pharmacological Zn or Cu) or positive [PC; Neo-Terramycin phases 1 and 2 (827 and 551 ppm) and Carbadox phase 3 (55 ppm)] control diet. Pigs from sows fed DFM, OA, or DFM+OA were fed the NC diet or a diet representative of their dam. Diets with DFM contained 1.6x109 CFU/kg DFM and diets with OA contained 0.5, 0.4, and 0.3% OA in phases 1-3, respectively. Weaning weight was used as a covariate for nursery performance. For all phases and overall, PC fed pigs had greater ADG, ADFI, and G:F (P < 0.05). Feeding DFM or OA in sow diets improved (interaction; P< 0.042) nursery pig G:F, but DFM+OA offspring had no improvement for d7-14, 0-14, and 0-21 G:F. Feeding DFM or OA to sows and their progeny decreased ADFI (interaction; P < 0.042) but improved G:F (interaction; P < 0.028) for d7-14 and 0-14 with DFM+OA having no improvement above CON. For d14-21 and 0-21, feeding DFM or OA to sows and their progeny decreased ADFI whereas DFM+OA increased ADFI above CON (interaction; P < 0.019). In conclusion, feeding DFM or OA to sows or their offspring may improve nursery feed efficiency and feeding DFM+OA diet to sows and their progeny may increase ADFI late in the nursery period. [ABSTRACT FROM AUTHOR]

Forty-seven sows and their progeny were used to determine if feeding gestating and lactating sows a Bacillus licheniformis direct-fed microbial (DFM), an organic acid blend of medium chain and short chain fatty acids (OA), or in combination improves sow lactation feed and water intake, litter growth, and subsequent reproductive performance. On approximately d80 of gestation, sows were fed one of four diets in a 2 x 2 factorial design: 1) gestation control (CON; 0.55% SID Lysine), 2) CON with DFM (1.6x109 CFU/kg of complete feed), 3) CON with 0.4% OA, 4) CON with both DFM and OA. Dietary treatments were also fed throughout lactation (1.00% SID Lysine) starting on approximately d112 of gestation when sows entered farrowing facility. There was a tendency (P = 0.079) for DFM to decrease the amount of sow body weight loss in lactation by approximately 6% compared to sows not consuming the DFM, likely related to DFM sows numerically (P = 0.124) consuming 8.4% more feed during d7-14 of lactation. Sows fed the OA diets had fewer mummies/litter (P = 0.038) compared to diets not containing OA. Sows fed diets with the DFM gave birth to lighter pigs born alive (P = 0.003) compared to non-DFM fed sows, and a tendency for an interaction (P = 0.092) existed where feeding OA+DFM lessened the decrease in born alive BW. There was an interaction tendency (P = 0.133) where sows fed DFM returned to estrus 22 hours sooner than CON, but only 8 hours sooner when sows were fed the OA+DFM diet. In conclusion, feeding a Bacillus licheniformis DFM to sows may decrease pig born alive weight but reduce sow BW loss through 6.4% more lactation feed intake, quickening the return to estrus. Feeding the OA alone or in combination did not improve sow reproductive and litter growth performance in this study, and may require a larger sample size. [ABSTRACT FROM AUTHOR]

This study evaluated the effects of a liquid prototype (LIQP) and dry (XPC®; Diamond V) Saccharomyces cerevisiae feed additives on blood and uterine cytokine profiles in sows (n = 40). On d 112 of pregnancy sows were allotted to dietary treatments: 1) Control diet (CON), 2) CON +15 mL of LIQP (LIQ), 3) CON +0.20% of XPC (DRY), and 4) DRY +15 mL of LIQP until d 7 post-farrowing (D+L). Interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-10, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) were quantified from serum samples collected on d 112 of gestation, d 2 and 6 post-farrowing, and from uterine fluid collected on d 2, 4, and 6 post-farrowing. Serum C-Reactive protein (CRP) and haptoglobin concentrations were evaluated. No interactions between treatments and day of collection were observed (P > 0.13). LIQ and D+L sows had the greatest serum IL-10 concentration (P < 0.001) and sows fed CON tended to have lower concentration of IL-8 (P < 0.06) vs. other treatments. Serum CRP concentrations were greatest on d 2 (P < 0.001), serum IL-10 (P < 0.04) and IL-4 (P < 0.07) linearly decreased while serum haptoglobin (P < 0.02) and INF-γ (P < 0.001) linearly increased post-farrowing. In the uterine fluid, LIQ and D+L sows had greater INF-γ (P = 0.04) concentrations and CON tended to have the least concentration of TNF-α (P = 0.08). Uterine fluid IL-1 tended to linearly increase (P < 0.07) and IL-6 linearly decrease (P < 0.01) post-farrowing. No strong correlations were detected between cytokines in the serum and uterine fluid within day. LIQ sows had the greatest daily feed intake and CON the least during the first week of lactation (P = 0.04). Providing LIQP post-farrowing to sows modified immune response increasing both pro- and anti-inflammatory cytokines in serum and uterine fluid in an independent manner, allowing animals a quicker recovery and increased feed intake. [ABSTRACT FROM AUTHOR]

Trace element and mineral intake are important for proper function of the immune system and various metabolic processes. The objective of this experiment was to evaluate the effect of a dacitic tuff breccia (DTB), classified as a hydrated sodium calcium aluminosilicate, in the diet of nursery pigs. Dietary treatments were: 1) Control diet (CON), 2) CON + 0.25% DTB (DTB1), and 3) CON + 0.50% DTB (DTB2). Pigs (6.79 + 0.064 kg; 26d of age) were individually housed and fed dietary treatments for 21 d (n=48; 16/trt). At the end of the experiment, blood, jejunal, and ileal samples were collected for a typical metabolic panel and basic histologic and morphologic measurements. DTB2 fed pigs tended (P=0.07) to have greater ADG than CON and DTB1 fed pigs (392, 397, 430g/d for CON, DTB1, DTB2, respectively), and greater (P=0.008) ADFI (591, 592, 669g/d for CON, DTB1, DTB2, respectively). Gain:Feed was not different (P=0.75) among treatments (0.655, 0.663, 0.644 for CON, DTB1, DTB2, respectively). Crypt depth in the jejunum was greater (P=0.04) for DTB1 compared to CON fed pigs but did not differ from DTB2 fed pigs (254.7, 292.0, 275.6mm for CON, DTB1, DTB2, respectively). Villous height, villous:crypt, number of goblet cells and intraepithelial lymphocytes did not differ among treatments in both ileum and jejunum (P >0.17). Total white blood cell (WBC) count did not differ (P >0.10). However, the proportion of WBC that were monocytes was lower (P=0.024) in DTB1 compared to CON and DTB2 fed pigs (1.27, 0.79, 1.45% for CON, DTB1, DTB2, respectively). Blood Urea Nitrogen (BUN) concentration tended (P=0.06) to be linearly decreased with increasing DTB concentrations (9.27, 8.80, 7.75mg/dL). DTB inclusion at 0.5% in nursery pig diets tended to improve growth performance, while inclusion at 0.25% increased jejunal crypt depth and reduced monocytes:WBC and increasing DTB concentrations tended to decreased BUN linearly. [ABSTRACT FROM AUTHOR]

In utero heat stress (IUHS) reduces swine productivity and welfare but little is known about the long-term impact on immune function. The study objective was to determine the effects of IUHS on the immune and metabolic response of pigs subjected to an 8 h lipopolysaccharide (LPS) challenge during postnatal life. Twentyfour pregnant gilts were exposed to thermoneutral (TN; n = 12; 17.5 ± 2.1⁰C) or heat stress (HS; n = 12; cyclical 27°C-37°C) conditions from d 1 to 58 of gestation, and TN conditions from d 59 of gestation to farrowing. After farrowing, all piglets were housed under the same conditions. At 12 weeks post-farrowing, 16 IUHS and 16 in utero thermoneutral (IUTN) pigs were selected, balanced by sex and given an intravenous injection of LPS (2 μg/kg BW) or saline (SAL). Treatment combinations were: IUTN-SAL, IUTN-LPS, IUHS-SAL, IUHS-LPS. Body temperature was monitored in 30-min intervals and blood samples were obtained at 0, 1, 2, 3, 4, 6, and 8 h. Blood was analyzed for glucose, insulin, non-esterified fatty acids (NEFA), and cytokine concentrations. Body temperature increased (P < 0.01; 1.05°C) in LPS versus SAL pigs, regardless of in utero treatment. Glucose concentrations were reduced overall (P = 0.05; 5.9%) in IUHS versus IUTN pigs. Non-esterified fatty acid concentrations tended to be greater (P = 0.07; 143.4%) in IUHS-LPS pigs compared to all other treatments, and IUTN-LPS pigs tended to have greater circulating NEFA concentrations (127.4%) compared to IUTN-SAL and IUHS-SAL pigs. At 1 h, TNFα was increased (P = 0.01; 115.1%) in IUHS-LPS compared to IUTN-LPS pigs. Overall, IL-1β and IL-6 were greater (P < 0.04; 56.0 and 46.8%, respectively) in IUHS-LPS compared IUTN-LPS pigs. In summary, IUHS altered the postnatal immune and metabolic response of pigs during postnatal life, which has negative implications towards future disease susceptibility. [ABSTRACT FROM AUTHOR]

Inflammatory mediators, such as cytokines, can reduce feed intake and drastically modify the partitioning of nutrients. Thus, it may increase the animal's maintenance requirements, reduce milk production and piglet growth, impact reproductive performance, and reduce sow lifetime productivity. The objective of this experiment was to evaluate a non-invasive method, not tested before in swine, to assess cytokine profiles from post-partum uterine lavage. The uteri of second and third parity sows (n=14) were flushed with sterile saline solution (0.9%) on days 2, 4, and 14 postparturition. An artificial insemination (AI) catheter, in its plastic covering, to avoid vaginal contents, was inserted through the vagina into the cervix. A flexible inner catheter was passed through the outer AI catheter and into the uterine horn. Sterile saline (30-40 mL) was flushed through the inner catheter into one uterine horn. After inner catheter removal, a tube was attached to the end of the outer AI catheter to allow collection of the natural back-flow of uterine lavage fluid (avg 24.7% recovery). Uterine fluid collected was immediately centrifuged and the supernatant stored at -20°C. Samples were freeze-dried, re-suspended in sterile saline (2 mL), and stored at -80°C. Cytokine profiles of the uterine fluid were evaluated using a multiplex ELISA panel including interleukin (IL)-1β, IL-6, IL-8, IL-4, IL-10, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ). Cytokine concentrations were calculated relative to protein content (pg/mg of protein). IFN-γ and TNF-α were lower than the limit of detection in most samples (5/38 and 1/38, respectively). IL-4 and IL-10 concentrations did not differ among days of collection (P >0.14). IL-8 was greater on day 4 than on days 2 or 14 (P< 0.05). IL-1β and IL-6 were greater on days 2 and 4 than on day 14 (P< 0.05). Uterine flushing is a novel non-invasive method to access uterine inflammatory cytokines in sows. [ABSTRACT FROM AUTHOR]

A total of 606 sows (PIC 1050) and their progeny (PIC 1050×280) were used to determine if feeding gestating and lactating sows a proprietary strain of Pichia guilliermondi as a whole-cell inactivated yeast product (WCY; CitriStim, ADM Animal Nutrition, Quincy, IL) improves sow and litter performance in a commercial production system. Once confirmed pregnant at d 35 post-breeding pregnancy check, sows were fed either a basal gestation control (CON) diet (0.55% SID Lysine) or the control diet fortified with 0.15% (1.36 kg/ton) of the WCY replacing corn in the CON diet. Dietary treatments were also fed in lactation (1.05% SID Lysine) once sows were moved into farrowing crates on approximately d 112 of gestation until weaning. Sows supplemented with WCY in gestation and lactation had a greater number of total born piglets by 0.45 pigs (P=0.040), born alive (P< 0.04), heavier born alive litter weight (P< 0.001), and greater post cross-foster litter size (P< 0.001) compared to CON fed sows. Litter size at weaning was increased by 0.54 pigs when sows were fed WCY compared to CON (P< 0.001). However, the average piglet weaning weights from CON fed sows were heavier by 0.35 kg compared to WCY (P< 0.001). This increase in body weight of piglets from CON fed sows is partially explained by their 0.93 days longer lactation (P< 0.001), and may also be due to the smaller litter size nursed throughout lactation. The percent of litters treated for scours decreased from 38.3% to 14.2% when sows were fed WCY (P< 0.001). The distribution of birth weights by body weight range was not different (P=0.2461) between treatments. In conclusion, feeding gestating and lactating sows a proprietary strain of Pichia guilliermondi as a whole-cell inactivated yeast product increased the number of piglets born and number weaned as well as decreased the prevalence of scours during lactation. [ABSTRACT FROM AUTHOR]

For proper function of many metabolic processes trace element and mineral intake is important. The objective of this experiment was to evaluate the addition of a dacitic tuff breccia (DTB), a hydrated sodium calcium aluminosilicate, on nursery pig growth and nutrient digestibility. Dietary treatments were: 1) Control (CON) and 2) CON+0.50% DTB (DTB). Pigs (7.73±0.205 kg; 26 d of age) were individually housed and fed for 20d (n=24, 12/trt). Nutrient digestibility was evaluated using individual metabolism pens for 3d total collection of feces and urine during the last week of evaluation. DTB had no effect on pig performance during week 1 (P >0.28). Week 2 ADG (128 versus 302 g/d; P=0.02), BW (10.63 versus 11.77 kg; P=0.03), and G:F (0.275 versus 0.608; P=0.02) were improved for DTB compared to CON fed pigs. Week 3 ADFI was greater for DTB fed pigs compared to CON (642 vs 768 g/d; P=0.01). Overall (d0-20), DTB fed pigs had greater ADG (279 versus 352 g/d; P=0.04), final BW (13.3 versus 14.8 kg; P=0.04), and numerically greater ADFI (497 versus 558 g/d; P=0.16) and G:F (0.562 versus 0.624; P=0.22) compared to CON. During the collection period, ADG was numerically increased (396 versus 427 g/d; P=0.69) and ADFI (544 vs 668 g/d; P=0.03) was greater for DTB versus CON fed pigs. Nutrient digestibility was not affected by inclusion of DTB in the diet (P >0.05). Digestibility coefficients for CON and DTB, respectively, were: 0.863 and 0.856 for DM; 0.842 and 0.842 for digestible energy; 0.824 and 0.814 for nitrogen; 0.701 and 0.667 for phosphorus; 0.873 and 0.816 for sodium; 0.481 and 0.431 for zinc; 0.517 and 0.458 for iron; and 0.438 and 0.392 for manganese. DTB in nursery pig diets improved growth performance and tended to improve feed intake, but had no effect on nutrient digestibility. [ABSTRACT FROM AUTHOR]

In utero heat stress (IUHS) negatively affects swine productivity and is a growing concern for the swine industry. The study objective was to evaluate and mitigate the negative effects of IUHS on pig performance following weaning and transport. Based on previous research, we hypothesized that IUHS pigs would have reduced performance relative to in utero thermoneutral (IUTN) pigs due to greater maintenance costs and supplementing a nutrient-dense (ND) diet would improve IUHS pig performance to similar levels as IUTN pigs fed a common (C) diet. Therefore, 24 bred gilts were assigned to either thermoneutral (TN; n = 12; 17.5 ± 2.1°C) or heat stress (HS; n = 12; cyclical 27°C to 37°C) conditions for the first half of gestation (d1 to 58) and then TN conditions for the remainder of gestation. At weaning (16.2 ± 0.4 d), pigs (N = 160; 4.78 ± 0.15 kg BW) were transported (loading + transport + unloading) for approximately 12h. Pigs were then blocked into pens (n = 4 pigs/pen) by in utero and dietary treatments which were fed for 14 d (Period-1): IUTN-C (n = 10 pens), IUTN-ND (n = 10 pens), IUHS-C (n = 10 pens), IUHS-ND (n = 10 pens). Following Period-1, all pigs were fed the C diet from d15 to 35 post-weaning and transport (Period-2). Production measures were taken on d 0, 7, 14, 21, 28, and 35 post-weaning and transport to calculate ADG, ADFI, and G:F. During Period-1, ADG was reduced overall (P = 0.03, 10.6%) for IUHS pigs compared to IUTN pigs. Pigs fed ND diets had reduced ADFI during Period-1 (P = 0.02, 8.8%) relative to C fed pigs, regardless of in utero treatment. During Period-2, ADFI was increased (P = 0.03, 4.7%) in ND versus C pigs, regardless of in utero treatment. In summary, increasing the nutrient density of the diet did not rescue the lost productivity of IUHS pigs as indicated by an overall reduction in ADG. [ABSTRACT FROM AUTHOR]

Heat stress (HS) has depletory effects on intestinal morphology, induces metabolic changes, and disposes pigs to oxidative stress. Zinc has roles in gut health, nutrient and insulin mediated metabolism. To better understand the effects of cyclic heat and dietary Zn supplementation on gut integrity, carcass composition, and pork quality, commercial crossbred mixed-sex pigs (n = 400; initially 72.2 kg) were housed under either thermoneutral (TN; 18.9–16.7°C) or cycling HS conditions simulating chronic summer heat (30°C/26.7°C for 12h:12h on days 24–65) with acute heat periods of 32-33°C/29-30°C for 12h:12h on days 21–24, 42–45, and 63–65. Treatments were arranged in a 2×2×2 factorial with main effects of environment (HS vs. TN), added Zn level (50 vs. 130 mg/kg available Zn), and added Zn source (inorganic from ZnO vs. organic from Availa®Zn; Zinpro Corp, Eden Prairie, MN). Relative to TN, HS elevated (P < 0.050) body temperatures during the growing period. Heat stress was correlated with ileal villus height (r=-0.51, P = 0.015) and HSP-70 expression (r=0.46, P = 0.041). Growth was reduced such that carcasses from HS pigs were lighter (P = 0.011) and exhibited improved carcass quality with higher (P = 0.001) 24-hour loin pH, decreased (P = 0.034) drip loss, and greater (P < 0.050) subjective color and firmness scores compared to TN carcasses. Relative to TN, loin chops and sausage patties manufactured from the HS carcasses had similar oxidative stability (CIE L*a*b* color, 2-thiobarbituric acid reactive substances) throughout a 10-day simulated retail display. The HS induced fatty acid profile differences in the pork product but were not sufficient to shift IV (P > 0.10). Belly firmness and slice lean were also unaffected (P > 0.10) by HS. Zinc supplementation had minimal impact on the carcass quality characteristics studied. Further research is necessary to better understand the impact of HS duration, combination with additional stressors, and refine the ability to utilize thermal monitoring to manage negative impacts of HS. [ABSTRACT FROM AUTHOR]

Thirty-two barrows were used in a 35-d experiment with the objective of evaluating effects of supplemental soluble fiber (dextrin) pre- and post-weaning on growth performance and VFA production. Pigs were blocked by genetics and BW, and randomly allotted to treatments in a 2x2 factorial design with or without fiber pre-weaning and with or without fiber post-weaning. Fiber was suspended in chocolate milk and administered orally through a syringe from 14 d prior to weaning until 4 d post-weaning, after which it was included in the diet at 1%. At weaning, pigs were group housed by treatment and allowed ad libitum access to a common starter diet. On d 4 post-weaning, pigs were moved to individual pens and fed diets with or without 1% fiber. Weights and feed intake were recorded 14 and 3 d prior to weaning and on d 0, 4, 11, and 21 post-weaning. On d 21 post-weaning, pigs were euthanized, and large intestine contents were collected for VFA analysis. Data were analyzed using the GLM procedure of SAS with pig as the experimental unit. Growth performance was not affected (P > 0.10) by treatment. A trend for an interaction of pre- and post-weaning fiber supplementation was observed for acetate (P = 0.052) and butyrate (P = 0.087) concentrations in large intestine contents. Pigs fed fiber only in the nursery had the highest acetate concentration, while pigs never receiving fiber had the lowest concentration. Pre-weaning fiber supplementation followed by no fiber in the nursery resulted in the highest butyrate concentrations with all other treatments being similar. Fiber supplementation pre-weaning tended (P = 0.053) to reduce isobutyrate concentrations. Pigs not receiving fiber post-weaning had increased valerate concentrations (P = 0.0245). There were no changes observed in propionate or isovalerate concentrations (P > 0.10). Fiber supplementation caused alterations in VFA concentrations when fed during pre- and post-weaning, indicating possible shifts in the microbiome, immune status and barrier function of the intestinal tract. [ABSTRACT FROM AUTHOR]