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The Eurasian Scops Owl Otus scops is a migratory owl whose population is declining throughout a large part of its breeding range, due to intensification of farming practices and land-use changes. For this reason, it is considered Europe's most threatened owl species. Scops Owl is one of several raptor species that are difficult to sex, due to limited morphological differences between the sexes. A reliable method of sexing Scops Owls through biometric measurements would facilitate the study of their sex-related survival, movements and behaviour characteristics, improving their conservation management. In the current study, we developed a cost-effective and accurate method of sexing Scops Owls, based on discriminant analysis of morphometrics. A sample of 105 birds were captured, sexed using genetic methods and had biometric measures taken. A generalised linear model was built to evaluate the biometric measures that are statistically linked to sex. The best model selected three variables: wing length, tail length and weight. The results of the model were used to derive model scores, and the probability of male and female to be correctly identified was predicted. The discriminant function provides an accurate method of sexing Scops Owls in the hand. [ABSTRACT FROM AUTHOR]

The objective of this study was to demonstrate the usefulness of an immunomagnetic method to purify subpopulations of milk somatic cells. The experiment was conducted on milk samples collected from healthy cows (n = 17) and from cows with clinical mastitis (n = 24) due to a Staphylococcus aureus natural infection. A two-step immunomagnetic purification was applied to simultaneously separate three somatic cell subpopulations from the same milk sample. Total RNA was extracted and qPCR was performed to determinate mRNA levels of innate immunity target genes in purified somatic cell subpopulations. Good quality and quantity of RNA allowed the reference gene analysis in each cell subpopulation. An up-regulation of the main genes involved in innate immune defence was detected in separated polymorphonuclear neutrophilic leucocytes-monocytes and lymphocytes of mastitic milk. These results and flow cytometric analysis suggest that the immunomagnetic purification is an efficient method for the isolation of the three populations from milk, allowing the cells to be studied separately. [ABSTRACT FROM AUTHOR]

The aims of this investigation were to analyse the genetic variation of two Piemonte chicken local breeds, Bionda Piemontese and Bianca di Saluzzo, and to set them against some commercial lines. A panel of 19 microsatellite markers was used. On the overall, the results of different analyses highlight the genetic uniqueness of the two breeds; therefore they should be considered genetic resources worthy of preservation. The panel of microsatellites used in this investigation turns out to be a consistent and reliable tool for traceability. In fact, these markers are able to distinguish the two local populations from the commercial lines and they are able to confirm the existence of two genetically different clusters within the Bionda Piemontese, namely the ecotypes standard and Cuneo. Mating policies implemented to avoid inbreeding and, if necessary, a marker assisted conservation scheme would be sufficient to solve the problem of inbreeding. [ABSTRACT FROM AUTHOR]

Degenerative myelopathy (DM) is a late-onset, slowly progressive degeneration of spinal cord white matter which is reported primarily in large breed dogs. The missense mutation SOD1:c.118G> A is associated with this pathology in several dog breeds, including the German Shepherd Dog (GSD). The aims of the present study were to develop a tool for the rapid screening of the SOD1 mutation site in dogs and to evaluate the association of the polymorphism with DM in the German Shepherd breed. Two different techniques were compared: a minisequencing test and a real-time pcr allelic discrimination assay. Both approaches resulted effective and efficient. A sample of 47 dogs were examined. Ten subjects presented the symptoms of the illness; for one of them the diagnosis was confirmed by postmortem investigations and it resulted to be an A/A homozygote. In another clinically suspected dog, heterozygote A/G, the histopathological examination of the medulla showed moderate axon and myelin degenerative changes. GSD shows a frequency of the mutant allele equal to 0.17, quite high being a high-risk allele. Because canine DM has a late onset in adulthood and homozygous mutant dogs are likely as fertile as other genotypes, the natural selection is mild and the mutant allele may reach high frequencies. A diagnostic test, easy to implement, may contribute to control the gene diffusion in populations. The SOD1:c.118G> A mutation could be a useful marker for breeding strategies intending to reduce the incidence of DM. [ABSTRACT FROM AUTHOR]

The objective of this study was to describe the VNTR polymorphism of the mucin 1 gene ( MUC1) in three Nelore lines selected for yearling weight to determine whether allele and genotype frequencies of this polymorphism were affected by selection for growth. In addition, the effects of the polymorphism on growth and carcass traits were evaluated. Birth, weaning and yearling weights, rump height, Longissimus muscle area, backfat thickness, and rump fat thickness, were analyzed. A total of 295 Nelore heifers from the Beef Cattle Research Center, Instituto de Zootecnia de Sertãozinho, were used, including 41 of the control line, 102 of the selection line and 152 of the traditional. The selection and traditional lines comprise animals selected for higher yearling weight, whereas control line animals are selected for yearling weight close to the average. Five alleles were identified, with allele 1 being the most frequent in the three lines, especially in the lines selected for higher means for yearling weight. Heterozygosity was significantly higher in the control line. Association analyses showed significant effects of allele 1 on birth weight and weaning weight while the allele 3 exert significant effects on yearling weight and back fat thickness. Despite these findings, application of this marker to marker-assisted selection requires more consistent results based on the genotyping of a larger number of animals in order to increase the accuracy of the statistical analyses. [ABSTRACT FROM AUTHOR]

nalysis of the genetic diversity of the Alpine chamois in Italy was conducted using a pool of 26 microsatellite loci. A total of 209 animals were analyzed, representing six geographical populations from different location of the Southern slope of the Alps. Clear genetic differences have emerged between the sampled chamois groups. Some were consistent with an isolation-by-distance model. However, in parallel, other mechanisms intervened in areas that, in addition to being peripheral to the main alpine ridge, had suffered from recent bottlenecks. In such areas, genetic drift and a low rate of gene flow are likely explanations for the current genetic structure. [ABSTRACT FROM AUTHOR]

The aim of the present study was to assess whether individual Sarcoptes mites collected from frozen skin ('postponed isolation' method) are suitable sources of PCR-quality genomic DNA, and to test the effectiveness of this method in comparison with the 'direct isolation' method, often used through force of habit. Hundreds of single Sarcoptes scabiei samples, resulting from direct (live) or postponed (post-frozen) isolation, were tested using a ∼450 bp product (ITS-2) and multi-locus 10× genotyping with microsatellite markers. No statistical difference in yield of soluble DNA was found between the two isolation methods. Nevertheless, 19% of the reactions were classified as failed preparations in the direct isolation method, whereas the rate of unsuccessful reactions was 34% in the postponed isolation method. Consequently, post-frozen isolation is suitable and recommendable for Sarcoptes mite gDNA preparation, particularly when performing a balancing act among safety, practicability and profitability. These results have implications for mite collection for DNA extraction, and hence the needed wider leap of Sarcoptes into the genetic era. [ABSTRACT FROM AUTHOR]

Simple Summary: This study examined the impact of whole sesame seeds (WSS), rich in both linoleic acid and lignans, on the innate immunity of goats. WSS were incorporated in the concentrates of the control group at 5 and 10% respectively, by partial substitution of both soybean meal and corn grain. The highest supplementation level of WSS resulted in a significant down-regulation in the expression levels of several pro-inflammatory genes in the neutrophils of goats. In conclusion, the dietary supplementation of goats with WSS might be a good nutritional strategy to improve their innate immunity. Whole sesame seeds (WSS) are rich in both linoleic acid (LA) and lignans. However, their impact on the innate immunity of goats is not well studied. Twenty-four goats were divided into three homogeneous sub-groups; comprise one control (CON) and two treated (WWS5 and WWS10). In the treated groups, WSS were incorporated in the concentrates of the CON at 5 (WSS5) and 10% (WSS10) respectively, by partial substitution of both soybean meal and corn grain. The expression levels of MAPK1, IL6, TRIF, IFNG, TRAF3, and JUND genes in the neutrophils of WSS10 fed goats were reduced significantly compared with the CON. The same was found for the expression levels of IFNG and TRAF3 genes in the neutrophils of WSS5 fed goats. Both treated groups primarily affected the MYD88-independent pathway. The dietary supplementation of goats with WSS might be a good nutritional strategy to improve their innate immunity. [ABSTRACT FROM AUTHOR]

Simple Summary: Mastitis is a worldwide diffused disease usually treated with an excessive use of antibiotics. Therefore, antimicrobial resistance is an important issue to be addressed by scientists. One of the possible solutions to decrease the use of drugs is genetic selection of resistant animals, that is, individuals that can be more resistant to mastitis. In our survey we analyzed Single Nucleotide Polymorphisms (SNPs) in genes known to be involved in both infection resistance and immune system activity. We found a group of SNPs that can be associated to mastitis related phenotypes (namely SCS) and that can be used for selecting resistant animals. An efficient selection is able to improve both animal welfare and quality and safety of animal products Mastitis is an infectious disease affecting the mammary gland, leading to inflammatory reactions and to heavy economic losses due to milk production decrease. One possible way to tackle the antimicrobial resistance issue stemming from antimicrobial therapy is to select animals with a genetic resistance to this disease. Therefore, aim of this study was to analyze the genetic variability of the SNPs found in candidate genes related to mastitis resistance in Holstein Friesian bulls. Target regions were amplified, sequenced by Next-Generation Sequencing technology on the Illumina® MiSeq, and then analyzed to find correlation with mastitis related phenotypes in 95 Italian Holstein bulls chosen with the aid of a selective genotyping approach. On a total of 557 detected mutations, 61 showed different genotype distribution in the tails of the deregressed EBVs for SCS and 15 were identified as significantly associated with the phenotype using two different approaches. The significant SNPs were identified in intergenic or intronic regions of six genes, known to be key components in the immune system (namely CXCR1, DCK, NOD2, MBL2, MBL1 and M-SAA3.2). These SNPs could be considered as candidates for a future genetic selection for mastitis resistance, although further studies are required to assess their presence in other dairy cattle breeds and their possible negative correlation with other traits. [ABSTRACT FROM AUTHOR]

Simple Summary: Bianca di Saluzzo and Bionda Piemontese are two Italian slow-growing chicken breeds of the Piemonte region (Northwest Italy) and are reared mainly for meat. They conserve adaptation to free ranging low input rearing systems and are promising for antibiotic-free farming. We aimed to analyze their growth using a mathematical model and to obtain some advice for improving performance. Polymorphism of the PAX7 gene was also studied to increase conservation value. The results confirmed that these breeds are late-maturing. Whereas selection would increase inbreeding, a mating scheme to bring inbreeding under control could be the most proper way to increase growth rate and reach commercial maturity earlier. Bianca di Saluzzo (BS) and Bionda Piemontese (BP) are two Italian chicken breeds, mainly reared for meat production, primarily in antibiotic-free farming. However, technical information on their growth pattern is still missing. At hatching, 150 unsexed chicks of each breed were weighed, labeled, and reared in indoor pens up to 8 w of age. At 8 w of age, the chicks were separated by sex and randomly transferred to growing pens with access to an external paddock (15 birds/pen; 4 pens/sex for each breed). The body weight (BW) was recorded biweekly for each bird, from hatching to 32 w of age. In order to identify an improvement strategy, the objectives of our study were to analyze the growth pattern of these birds using the Gompertz mathematical model and compare results with other chicken breeds. Polymorphism of the PAX7 gene was also analyzed to test its association with growth traits. Both BS and BP are close to unselected native breeds and, among the Italian local poultry, they are confirmed to be slow-growing birds with an intermediate size between heavy and light chicken breeds. Regarding the PAX7 gene, two alleles were found, F and G, and showed an association with the actual BW in the BP females from 14 w of age onwards. The G allele always exhibited a more favorable effect than the F allele. In small size poultry population, a delicate balance between preservation of biodiversity and performance improvement should be considered. Consequently, the most proper way could be an approach based on a mating scheme to keep inbreeding under control, increase growth rate, and improve commercial maturity. [ABSTRACT FROM AUTHOR]

The European Union has implemented breeding programmes to increase scrapie resistance in sheep. A similar approach can be applied also in goats since the K222 allele provides a level of resistance equivalent to that of ARR in sheep. The European Food Safety Authority stated that breeding for resistance could be offered as an option for Member States to control classical scrapie in goats. We assessed the impact of different breeding strategies on PRNP genotype frequencies using a mathematical model that describes in detail the evolution of K222 in two goat breeds, Chamois Coloured and Saanen. Different patterns of age structure and replacement rate were modelled as factors affecting response to selection. Breeding for scrapie resistance can be implemented in goats, even though the initial K222 frequencies in these breeds are not particularly favourable and the rate at which the resistant animals increase, both breeding and slaughtered for meat production, is slow. If the goal is not to achieve the fixation of resistance allele, it is advisable to carry out selection only until a desired frequency of K222-carriers has been attained. Nucleus selection vs. selection on the overall populations is less expensive but takes longer to reach the desired output. The programme performed on the two goat breeds serves as a model of the response the selection could have in other breeds that show different initial frequencies and population structure. In this respect, the model has a general applicability. [ABSTRACT FROM AUTHOR]