Search

Ouabain-sensitive Na,K-ATPase (NKA) catalyses active Na/K exchange, and it is responsible for the NaK gradient across the plasma membrane, cell volume control and membrane excitability and it has been associated to nociception in the spinal dorsal horn (SDH). Several tissues show diurnal changes in NKA activity with important physiological consequences. To test the possibility of diurnal changes in NKA activity in the SDH, every 2 hours, 2 adult (~300 g) male Sprague-Dawley rats housed at a 12: 12 h light: darkness schedule were anesthetized (thiobarbital) and laminectomized (T-L), to extract the lumbar spinal cord. Micropunches of SDH (1 mm ø) were taken from coronal sections of the spinal cord, and their homogenates were tested for protein concentration and NKA activity. The NKA activity exhibited a highly significant rhythm with a 21.2 h period ( P < 0.005; robustness = 57.9%, diurnal fit P < 0.02), a peak at 15.93 h and a 24 h mean value of 638.3 nmol P mg prot min, with an amplitude of oscillation of 71.0 nmol P mg prot min. Light hours show significantly higher ATPase activity values than dark hours (660.9 ± 51.0 vs 608.4 ± 12.8 nmol P mg prot min; P = 0.03). Thus, ouabain-sensitive NKA in SDH displays an intense diurnal activity that could be an important factor in pain and sensory diurnal changes. [ABSTRACT FROM AUTHOR]

In the present work, a Mg [sup 2++] -dependent, Ca [sup 2+] -stimulatedATPase activity was determined and characterized in purified preparationsof syncytiotrophoblast basal (fetal facing) plasma membranes, and its characteristicswere compared to those of the active Ca [sup 2+] -transport already demonstratedin this tissue. Similar to the active Ca [sup 2+] transport, the Ca-ATPaseis Mg [sup 2+] -dependent, is stimulated by calmodulin, and is inhibitedby vanadate. The K[sub m] for Ca [sup 2+] activation is 0.25±0.02µM, a value near to that described for calcium active transportin this tissue. Consequently, the Ca-ATPase activity of human syncytiotrophoblastbasal plasma membrane described in this paper could be responsible for theactive extrusion of calcium from the syncytiotrophoblast toward the fetalcirculation. [ABSTRACT FROM AUTHOR]