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Although the European flounder is frequently used in research and has economic importance, there is still lack of comprehensive transcriptome data for this species. In the present research we show RNA-Seq data from ten selected organs of P. flesus female inhabiting brackish waters of the Gulf of Gdańsk (southern Baltic Sea). High throughput Next Generation Sequencing technology NovaSeq 6000 was used to generate 500 M sequencing reads. These were mapped against European flounder reference genome and reads extracted from the mapping were assembled producing 61k reliable contigs. Gene ontology (GO) terms were assigned to the majority of annotated contigs/unigenes based on the results of PFAM, PANTHER, UniProt and InterPro protein databases searches. BUSCOs statistics for eukaryota, metazoa, vertebrata and actinopterygii databases showed that the reported transcriptome represents a high level of completeness. The data set can be successfully used as a tool in design of experiments from various research fields including biology, aquaculture and toxicology. [ABSTRACT FROM AUTHOR]

Background: The European flounder is readily chosen as an experimental subject and model in physiological and ecotoxicological studies mostly because of its adaptability to laboratory conditions. Many studies utilise a quantitative PCR (qPCR) approach to ascertain the expression of target genes under experimental conditions. Such an approach relies heavily on the selection of reference genes with stable expression. Yet certain housekeeping genes are commonly used in this role, often without due consideration of their overall expression patterns. Therefore, new approaches should be developed to identify stable reference genes for a given species and to expand the general pool of genes suitable for the reference in qPCR analysis. Methods and results: Here RNA-seq data of nine flounder organs led to identify four candidate genes of the most stable expression. It was achieved by differential expression analysis and tritoconstrictor script. Specific primers were designed for the complete ORF as well as for qPCR analysis. RT-qPCR efficiencies were tested on ORF amplicon templates. Most of the genes tested showed good amplification in a wide range of template dilutions (107-101), with a correlation coefficient (R2) ranging from 0.991 to 0.998 and a consistent efficiency (E) (Sybr Green I staining and TaqMan molecular probe). Conclusions: The proposed approach based on differential expression analysis and a new bioinformatic tool is an appropriate selection method of candidates for reference genes in qPCR. The proposed approach, combining differential expression analysis with a new bioinformatics tool, provides an effective method for selecting reference gene candidates for qPCR. As a result, we can propose four genes (polr2f, yif1a, sf3b6, uba52), each with a set of validated primers, as suitable for consideration as reference genes in qPCR analysis in European flounder, an emerging model species. [ABSTRACT FROM AUTHOR]

Simple Summary: Simple Summary: Poultry can be exposed to a number of adverse stimuli during the pre-slaughter handling. Noise, movement, lack of access to water and feed, change in habitat, disruption of flock hierarchy or environmental conditions can lead to adverse changes in the bird's body. A significant increase in these factors can cause disturbances in the body's homeostasis. These changes may be reflected in meat quality. Therefore, there is a need to assess the effect of pre-slaughter turnover on meat quality. In this study, the effects of pre-slaughter treatments on changes in macro- and micronutrient content were analyzed. In this study, the effect of pre-slaughter handling on the content of macro- and micronutrients in blood serum and in the breast muscle of turkeys was assessed. Four different variants of pre-slaughter handling were used in the research: no transport (N-T), transport for a distance of 100 km (T-100), transport for a distance of 200 km (T-200), and transport for a distance of 300 km (T-300). In each of them, 30 female and 30 male turkeys were used. Blood was collected from the birds before slaughter, and samples of the pectoral muscle were collected after slaughter. In the obtained biological material, the content of Mg, P, Ca, Fe, Na and K was analyzed. On the basis of the obtained research results, it can be concluded that the pre-slaughter handling negatively affects the content of macro- and microelements both in the blood serum and in the pectoral muscles of experimental turkeys. Additionally, differences due to the sex of birds were observed. [ABSTRACT FROM AUTHOR]

UPF3B encodes the Regulator of nonsense transcripts 3B protein, a core‐member of the nonsense‐mediated mRNA decay pathway, protecting the cells from the potentially deleterious actions of transcripts with premature termination codons. Hemizygous variants in the UPF3B gene cause a spectrum of neuropsychiatric issues including intellectual disability, autism spectrum disorder, attention deficit hyperactivity disorder, and schizophrenia/childhood‐onset schizophrenia (COS). The number of patients reported to date is very limited, often lacking an extensive phenotypical and neuroradiological description of this ultra‐rare syndrome. Here we report three subjects harboring UPF3B variants, presenting with variable clinical pictures, including cognitive impairment, central hypotonia, and syndromic features. Patients 1 and 2 harbored novel UPF3B variants—the p.(Lys207*) and p.(Asp429Serfs*27) ones, respectively—while the p.(Arg225Lysfs*229) variant, identified in Patient 3, was already reported in the literature. Novel features in our patients are represented by microcephaly, midface hypoplasia, and brain malformations. Then, we reviewed pertinent literature and compared previously reported subjects to our cases, providing possible insights into genotype–phenotype correlations in this emerging condition. Overall, the detailed phenotypic description of three patients carrying UPF3B variants is useful not only to expand the genotypic and phenotypic spectrum of UPF3B‐related disorders, but also to ameliorate the clinical management of affected individuals. [ABSTRACT FROM AUTHOR]

Benzophenones (BPs) are substances used in the production of sunscreens, cosmetics, and personal care products. However, there is a lack of knowledge of BPs in wild animals. Therefore, the study aimed to assess the concentration of selected BPs commonly used in the cosmetic industry in guano samples collected from 4 colonies of greater mouse-eared bats (Myotis myotis). Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to determine guano concentrations of benzophenone 1 (BP-1), benzophenone 2 (BP-2), benzophenone 3 (BP-3) and benzophenone 8 (BP-8). BP-1 levels above the method quantification limit (MQL) were noted in 97.5% of samples and fluctuated from <0.1 ng/g to 259 ng/g (mean 41.50 ng/g, median 34.8). The second most common was BP-3, which fluctuated from <0.1 ng/g to 19 ng/g (mean 6.67 ng/g, median 5.05), and its levels higher than MQL were observed in 40% of samples. BP-2 and BP-8 concentrations did not exceed the method detection limit (0.04 ng/g) in any analyzed sample. There were visible differences in the BP-1 and BP-3 levels among the studied bat colonies. Mean BP-1 concentration fluctuated from 11.23±13.13 ng/g to 76.71±65.51 ng/g and differed significantly between the colonies. Mean BP-3 concentration fluctuated from 5.03±6.03 ng/g to 9.18±7.65 mg/g, but it did not differ significantly between the colonies. The results show that guano is a suitable matrix for the assessment of wildlife exposure to BPs. This could be particularly advantageous in protected species, where not disturbing and stressing the animals are crucial. [ABSTRACT FROM AUTHOR]

A growing body of literature has shown that type 1 diabetes (T1D) and high-fat diet (HFD) affect female reproductive function and may be involved in a chronic inflammatory state. Our previous studies indicated that T1D as well as HFD may evoke perturbations in the receptor for advanced glycation end products (RAGE) signaling pathway. The aim of the study was to determine the amount of RAGE protein and its proinflammatory ligands in uterine tissues harvested from T1D and HFD/pre-diabetic mice (n = 5 per group). We sought the impact of T1D and HFD on the activity of the RAGE signaling pathway in uterine tissues during the estrous cycle. The abundance of RAGE and its ligands were estimated using immunohistochemical staining. However, we also performed nerve conduction velocity studies to confirm diabetic neuropathy. The highest amount of RAGE and its ligands were observed in uterine tissues of T1D mice. Moreover, myometrial activity of the RAGE signaling pathway was increased in HFD in comparison to the control group (P≤0.05). We observed a strong relationship between RAGE, Nε-(carboxymethyl)lysine (CML) and tumor necrosis factor alpha (TNFα) proteins in mice myometrium. These data suggest that T1D and HFD could modulate the activity of RAGE and thus RAGE signaling pathway in uterine tissues during estrous cycle. Long-term diabetes and HFD may induce malfunctions in the uterine milieu. In the future RAGE protein may serve as a molecular marker in the diagnosis of malfunctions in pre- and diabetic uterus milieu. [ABSTRACT FROM AUTHOR]

Background: Data regarding congenital sensorineural deafness (CSD) in client‐owned, white Devon Rex cats is limited because most of the information on this disease comes from experiments on mixed‐breed cats. Objectives: Provide data on the occurrence of CSD in a population of client‐owned purebred white Devon Rex cats. Animals: Forty client‐owned, purebred, white Devon Rex cats examined at 2 different facilities. Median age of the examined cats was 19 weeks. Methods: Hearing status was defined by use of brainstem auditory evoked responses. Results: The occurrence of sensorineural deafness in the studied population of Devon Rex cats was estimated at 10%. Unilateral and bilateral deafness occurred equally often, with 2 individuals having each (ie, 5.0%). No association between the occurrence of CSD and sex could be found, χ2 (1, n = 40) = 0.001 (P >.99). No association between blue irises and deafness was noted in the studied population, χ2 (1, n = 40) < 0.01 (P >.99). Conclusions: The occurrence of CSD in a population of client‐owned, white Devon Rex cats was found to be lower compared with data obtained in previously conducted studies of deafness in purebred cats. In the studied population of Devon Rex cats, no association between blue irises and CSD was found. [ABSTRACT FROM AUTHOR]

The aim of this study was to evaluate whether production costs can be reduced and whether the profitability of organic chicken fattening can be increased by selecting the appropriate genotypes. Rhode Island Red (K-11) and Sussex (S-66) conservative chicken genotypes, as well as modern chicken genotypes such as slow-growing Hubbard JA 957 hybrids and fast-growing Ross 308 hybrids, were selected for the study. One hundred and sixty chickens were used in the experiment (forty birds per group). The birds were fed commercial organic complete feed up to 52 days of age and organic farm-made feed (to reduce costs) between 53 and 81 days of age. Population distribution was determined using the Kolmogorov–Smirnov test. The results were analyzed statistically using one-way analysis of variance (ANOVA) with multiple comparisons and the post hoc Tukey test at a significance level of p < 0.05. The 81-day fattening period (the minimum recommended period in organic farming) led to considerable weight deficits in Rhode Island Red and S-66 chickens and excessive slaughter/trade weights in Hubbard JA 957 and Ross 308 chickens. The feed conversion ratio was high in Rhode Island Red (K-11) and Sussex (S-66) chickens at 4.19 and 4.50, respectively, and much lower in Hubbard JA 957 and Ross 308 chickens at 2.79 and 2.53, respectively. The choice of chicken genotypes had a major impact on the profitability of organic farming, and the total costs of feed and other ingredients per kg of body weight were determined at EUR 3.83 for Rhode Island Red (K-11), EUR 3.90 for Sussex (S-66), EUR 6.57 for Hubbard JA 957, and EUR 6.62 for Ross 308 genotypes. The profitability of organic farming can be increased by selecting modern, meat-type, slow-growing chicken genotypes. [ABSTRACT FROM AUTHOR]

This paper is devoted to the negative impact of transport activities. Every type of transport comes with negative effects on the environment, people's health, and people's comfort of living. Strategic papers from the European Union's (EU's) acts of law on national, regional, and local political levels have found that water transport is the future. The authors hypothesize that the increase in the use of inland waterway transport over the coming decades will provide an opportunity to increase the capacity of transport; however, without appropriate regulations regarding emission standards, the use of outdated water transport units will not allow us to achieve positive environmental effects. The authors, therefore, indicate good practices related to the use of shipping in conjunction with the use of modern types of propulsion. The literature review method was used to verify current knowledge about the impacts of water transport as well as to find possible alternatives. Moreover, a survey was conducted among experts—decision-makers in logistics companies operating in Polish seaports. The condition of ships, especially those used on inland waterways, needs to be improved, mainly in the aspect of emissions. Furthermore, a strategy should be prepared to include inland shipping as an ordinary part of the logistics chain in Poland, which seems to be a necessary and expected action, though it will bring only partial environmental benefits. [ABSTRACT FROM AUTHOR]

Multiple molecular pathways including the receptor for advanced glycation end-products-diaphanous related formin 1 (RAGE-Diaph1) signaling are known to play a role in diabetic peripheral neuropathy (DPN). Evidence suggests that neuropathological alterations in type 1 diabetic spinal cord may occur at the same time as or following peripheral nerve abnormalities. We demonstrated that DPN was associated with perturbations of RAGE-Diaph1 signaling pathway in peripheral nerve accompanied by widespread spinal cord molecular changes. More than 500 differentially expressed genes (DEGs) belonging to multiple functional pathways were identified in diabetic spinal cord and of those the most enriched was RAGE-Diaph1 related PI3K-Akt pathway. Only seven of spinal cord DEGs overlapped with DEGs from type 1 diabetic sciatic nerve and only a single gene cathepsin E (CTSE) was common for both type 1 and type 2 diabetic mice. In silico analysis suggests that molecular changes in spinal cord may act synergistically with RAGE-Diaph1 signaling axis in the peripheral nerve. Key messages: Molecular perturbations in spinal cord may be involved in the progression of diabetic peripheral neuropathy. Diabetic peripheral neuropathy was associated with perturbations of RAGE-Diaph1 signaling pathway in peripheral nerve accompanied by widespread spinal cord molecular changes. In silico analysis revealed that PI3K-Akt signaling axis related to RAGE-Diaph1 was the most enriched biological pathway in diabetic spinal cord. Cathepsin E may be the target molecular hub for intervention against diabetic peripheral neuropathy. [ABSTRACT FROM AUTHOR]

Telomerase activity has been found in the somatic tissues of rainbow trout. The enzyme is essential for maintaining telomere length but also assures homeostasis of the fish organs, playing an important role during tissue regeneration. The unique morphological and physiological characteristics of triploid rainbow trout, when compared to diploid specimens, make them a promising model for studies concerning telomerase activity. Thus, in this study, we examined the expression of the Tert gene in various organs of subadult and adult diploid and triploid rainbow trout females. Upregulated Tert mRNA transcription was observed in all the examined somatic tissues sampled from the triploid fish when compared to diploid individuals. Contrastingly, Tert expression in the ovaries was significantly decreased in the triploid specimens. Within the diploids, the highest expression of Tert was observed in the liver and in the ovaries of the subadult individuals. In the triploids, Tert expression was increased in the somatic tissues, while the ovaries exhibited lower activity of telomerase compared to other organs and decreased compared to the ovaries in the diploids. The ovaries of triploid individuals were underdeveloped, consisting of only a few oocytes. The lack of germ cells, which are usually characterized by high Tert expression, might be responsible for the decrease in telomerase activity in the triploid ovaries. The increase in Tert expression in triploid somatic tissues suggests that they require higher telomerase activity to cope with environmental stress and maintain internal homeostasis. [ABSTRACT FROM AUTHOR]

This review focuses on receptor for advanced glycation endproducts/diaphonous related formin 1 (RAGE/Diaph1) interaction as a modulator of actin cytoskeleton dynamics in peripheral nervous system (PNS) in diabetes. Deciphering the complex molecular interactions between RAGE and Diaph1 is crucial in expanding our understanding of diabetic length dependent neuropathy (DLDN). DLDN is a common neurological disorder in patients with diabetes. It is well known that actin cytoskeletal homeostasis is disturbed during DLDN. Thus, we review the current status of knowledge about RAGE/Diaph1 impact on actin cytoskeletal malfunctions in PNS and DLDN progression in diabetes. We also survey studies about small molecules that may block RAGE/Diaph1 axis and thus inhibit the progression of DLDN. Finally, we explore examples of cytoskeletal long‐non coding RNAs (lncRNAs) currently unrelated to DLDN, to discuss their potential role in this disease. Most recent studies indicated that lncRNAs have a great potential in many research areas, including RAGE/Diaph1 axis as well as DLDN. Altogether, this review is aimed at giving us an insight into the involvement of cytoskeletal lncRNAs in DLDN. [ABSTRACT FROM AUTHOR]

Background: Hamstring tendon grafts are the most common choice for anterior cruciate ligament (ACL) reconstruction (ACLR). Previous studies have provided evidence that offers conflicting opinions concerning the most favorable graft choice. Purpose: To identify whether the use of a quadrupled semitendinosus tendon (ST) or doubled semitendinosus tendon and gracilis tendon (ST/G) graft provides comparable anterior tibial translation (ATT) with similar functional results and similar donor site morbidity. Study Design: Randomized controlled trial; Level of evidence, 1. Methods: This was a prospective, patient- and surgeon-blinded, randomized trial set in a busy orthopaedic and traumatology department at a university hospital. Between 2015 and 2017, a total of 162 patients with ACL lesions were randomized to undergo ACLR with either a quadrupled ST or doubled ST/G graft. The primary endpoint was ATT assessed with the KT-1000 arthrometer. Clinical outcomes were assessed using the patient-reported outcome measures (PROMs) of the visual analog scale (VAS), International Knee Documentation Committee (IKDC) subjective evaluation form, Knee injury and Osteoarthritis Outcome Score (KOOS), Tegner activity scale, and Lysholm knee scoring scale. Results: Preoperative demographic data, ATT, and PROM scores showed no significant differences. At 4.5 years, no significant differences were found between the ST and ST/G groups with respect to PROM scores and KT-1000 arthrometer, Lachman test, and pivot-shift test findings. Differences in functional results between groups were significant (muscle strength). Subgroup analysis revealed significantly increased ATT in female patients undergoing ACLR with a quadrupled ST graft during the 4.5-year observation period, as assessed by the KT-1000 arthrometer, as well as inferior KOOS, IKDC, Lysholm, and VAS scores. Conclusion: This study showed a significant increase in ATT as well as inferior results on PROMs during a 4.5-year observation period in female patients undergoing ACLR with a quadrupled ST graft. In male patients, the study provided evidence of the noninferiority of ACLR with an ST graft, with no influence on donor site morbidity. Trial Registration: clinicaltrials.gov: NCT03626883 [ABSTRACT FROM AUTHOR]

Nearly Zero Energy Building (NZEB) is becoming a standard for new and renovated buildings throughout the European Union (EU). Through the ongoing implementation of directives related to energy efficiency and NZEB-compliant buildings, the EU commission has established that new and renovated NZEB-compliant buildings shall be implemented cost-effectively. This is assessed by linking the Life Cycle Cost (LCC) and energy demand calculations, representing them in a cost-optimality plot, and finding the optimal solution from the resulting Pareto front. Given that the results of an LCC calculation are quite dependent on the calculation model's scope and inputs, this study takes an explorative approach to determine the most influential parameters in LCC calculations for a pre-selected cost-effective package. This is achieved by varying the inputs using local and global variation methods. The local variation approach consists of varying the inputs one-at-a-time (OAT), whereas with global variation, all the selected inputs are variated simultaneously. The OAT approach identified the amount and unit cost of the utility supply (district heating, electricity, and gas) as the most influential parameters to the output. The OAT results were further used to rank the next five most sensitive parameters and perform a global sensitivity analysis using Monte Carlo (MC) simulations. A regression analysis of the MC results revealed high R2 values (≥0.98), suggesting a linear correlation between the output and the variable inputs. The sensitivity analysis determined the unit price of attic insulation, the gas price, and the lifetime of the Heat Pump (HP) as the most sensitive parameters in the three investigated models. [ABSTRACT FROM AUTHOR]

The main purpose of the paper is to present a methodical approach to differences in changes in intra-national potential accessibility. Research concerns level of accessibility and its dispersion, both for freight and passenger transport regarding four transport modes. The main added value of the paper is an indication of how to monitor changes in the accessibility of many modes of transport at the municipal level, as a result of investment processes, while taking into account the available data sources, both on the land use data and travel times, with the support of regional and central offices. We focus on the intensive development of transport infrastructure after Poland's accession to the EU. We conclude that outcomes of reducing territorial differences in accessibility are diverse depending on the transport mode: from a generally positive effect in passenger transport to varied effects in freight transport, including particularly highly polarising effects in rail transport. The research method provides the possibility of analysing multimodal changes in accessibility concerning numerous transport modes for any large country or group of countries. Certain development opportunities of the model to be implemented in the future are discussed. [ABSTRACT FROM AUTHOR]

Total knee replacement (TKA) is a frequent modality performed in patients with osteoarthritis. Specific circumstances can make it much more difficult to execute successfully, and additional procedures such as osteotomy may be required. The aim of this study was to perform a meta-analysis and systematic review of osteotomies combined with TKA. Methods: In June 2022, a search PubMed, Embase, Cochrane, and Clinicaltrials was undertaken, adhering to PRISMA guidelines. The search included the terms "osteotomy" and "total knee arthroplasty". Results: Two subgroups (tibial tubercle osteotomy and medial femoral condyle osteotomy) were included in the meta-analysis. Further subgroups were described as a narrative review. The primary outcome showed no significant difference in favor to TTO. Secondary outcomes showed improved results in all presented subgroups compared to preoperative status. Conclusion: This study showed a significant deficit of randomized control trials treated with osteotomies, in addition to TKA, and a lack of evidence-based surgical guidelines for the treatment of patients with OA in special conditions: posttraumatic deformities, stiff knee, severe varus, and valgus axis or patella disorders. [ABSTRACT FROM AUTHOR]

Background: Data on sensorineural deafness (CSD) in purebred white client-owned cats is limited as most of the information on this disease entity is assured from mixed-breed experimental colonies. It is known that cats with blue irises are more predisposed to CSD having been described as a condition in which many structures in the inner ear are damaged resulting in hearing loss. Cats with CSD are born deaf or lose their hearing irreversibly within the first 4-5 weeks of life. It is important to diagnose cats with this hereditary condition in order to eliminate affected individuals from breeding. The objectives of this study were to ensure data on prevalence of CSD in a population of 72 client-owned purebred white cats in Poland according to the color of the irises and to determine if there are any predispositions with regard to CSD among different breeds of cats in which the dominant W gene is present. Results: Conducted study included 72 purebred white cats from six different breeds. The prevalence of CSD in the conducted study was 16.7%, CI95 [8.9%; 23.3%]. Unilateral deafness (11.1%, CI95 [4.9%; 20.7%]) was more common than bilateral CSD (5.6%, CI95 [1.5%; 13.6%]). The studies did not show any association between sex and CSD, p =.46. No association between the blue color of irises and deafness in the studied population could be found, p =.91. When compared to the rest of the examined population, no association was found between CSD and a particular breed. Conclusions: Overall prevalence of CSD regarding the examined population of purebred client-owned cats was reported as lower when compared to previous studies concerning purebred cats. Cats with blue irises are more likely to be deaf in accordance to the current state of knowledge, however in the conducted study, no significant association between the presence of blue irises and deafness in white purebred cats could be identified. In order to eliminate CSD from the population, it is necessary to conduct examinations and diagnose CSD in white cats with blue irises as well as with irises of color other than blue. Association between particular breed and CSD wasn't identified. [ABSTRACT FROM AUTHOR]

The aim of this paper is to find the most important aspects of introducing zero-emission and low-carbon solutions in urban bus transport, as well as their availability. Beyond the economic aspects related to energy sources and consumption, the paper focuses on their impact on people and the environment. There is also an attempt to estimate the scale of the impact of changes in the structure of the energy sources of buses in urban transport compared with all the means of transport operating in a given area, as well as a justification for these changes. We also conducted a survey of bus passengers to check their awareness of the changes taking place and their attitude towards them. [ABSTRACT FROM AUTHOR]

There is very little research on the anthropometric and physiological profiles of lower-ranked young female athletes, even though, in most rowing clubs, such rowers constitute the vast majority. Therefore, this study investigated the anthropometric and physiological profiles of young Hungarian female rowers of different age categories and sports rankings (international vs. club). Anthropometric and physiological profiles were created for 36 junior (15–16 years), 26 older-junior (17–18 years), and 8 senior (19–21 years) female rowers who were club and international ranked members of seven of the largest Hungarian rowing clubs. Rowers >17-years-old with international rankings significantly outperformed their age-group peers with club rankings in terms of power, absolute VO2 max, and time to cover 2000 m, among other differences, but such differences were not observed with junior rowers. In all age groups, the length of the athletes' sports career was not significantly associated with differences in anthropometric and physiological characteristics. This study suggests that ranking is not associated with differences in the anthropometric and physiological characteristics of juniors. Thus, with non-elite juniors, it can be more difficult to predict competition outcomes based on differences in anthropometric and physiological profiles. [ABSTRACT FROM AUTHOR]

Simple Summary: It is important to determine the effects of thermal stress on the physiological parameters of obese persons. The aim of this study was to analyze the influence of sauna bathing on obese and non-obese physically inactive men. Sixty volunteers aged 18–24 years were divided into two groups (group I—normal body mass, group II—class 1 obesity). Their physiological parameters were monitored before and during the sauna session. The average values of body mass, body mass index, body surface area, and weight-to-height ratio were significantly higher in obese men than in the normal weight group. The values of physiological parameters were also significantly higher in obese individuals. However, the observed changes remained within the norm, which indicates that a 10 min sauna session is safe for young men regardless of their body fat levels. The effects of thermal stress on the physiological parameters of obese subjects remain insufficiently researched. The objective of this study was to determine the influence of sauna bathing on the physiological parameters of obese and non-obese physically inactive men. Sixty volunteers aged 18–24 years (20.85 ± 1.46) were divided into two groups (group I—normal body mass, group II—class 1 obesity) for a comparative analysis. Somatic features and body composition were determined before sauna, and blood pressure was measured before and after sauna. Physiological parameters were monitored before and during the sauna session. The average values of body mass, body mass index, body surface area, and weight-to-height ratio were significantly higher (p < 0.001) in obese men (by 28.39 kg, 8.7 kg/m2, 0.34 m2, and 0.13, respectively) than in the normal weight group. Similar observations were made in an analysis of the remaining body composition parameters. The values of physiological parameters (heart rate, systolic and diastolic blood pressure, energy expenditure, oxygen uptake, excess post-exercise oxygen consumption, respiratory rate) were significantly (p < 0.001) higher in obese subjects. However, the observed physiological changes were within the expected norm; therefore, a 10 min sauna session is safe for young men regardless of their body fat levels. [ABSTRACT FROM AUTHOR]

The article presents the relations between peasants and landlords in the period between the issuing of the December Decree (1807) and the publication of the June Act (1846). The constitution of the Duchy of Warsaw granted peasants personal freedom and announced the introduction of the Napoleonic Civil Code, which was to abolish feudalism. However, the December Decree, which was issued shortly afterwards, determined that squires should be considered owners of peasant farms, and not the peasant working there, according to the new capitalist understanding. In 1846, the so-called June Act significantly limited the possibility of ousting peasants by squires as it restored the concept of dominium directum et utile for farms with at least three acres of land. The aim of the present paper is to answer the question to what extent the Napoleonic Code affected the relations between the squires and the peasantry. A detailed analysis of the five contracts concluded at that time which are in the State Archive in Łódź suggests that modern French law had very little impact on property relations in the Polish countryside. In fact, the contracts between squires and peasants resulted in legal arrangements described as dominium directum et utile, although this institution was not even mentioned in the Napoleonic Code. [ABSTRACT FROM AUTHOR]

The present source publication is the sixth in a series encompassing the most important documents related to preparations for the replacement of French codes with national codification drafted by the Civil Reform Committee which worked between 1814 and 1815. The publication includes the second part of "The project of the civil judicial procedure in first instance", which is comprised of enforcement proceedings presented just like the first part (the trial) by Franciszek Grabowski, and the fragments of the minutes reporting the course of the discussion concerning the solutions proposed within the project. [ABSTRACT FROM AUTHOR]

Featured Application: The proposed method can be applied to a specific building renovation project for evaluating and selecting energy efficiency and renewable energy production measures, with the aim of reaching the NZEB standard. Reaching environmental targets set by the European Union (EU) requires a constant renovation of the existing building stock to nearly Zero Energy Buildings (NZEB) in a cost-optimal manner. Studies show that the renovation rate of the existing building stock is more than two times less than what is necessary to reach the targets. Furthermore, the majority of performed renovations across the EU reach just a small amount of energy savings, whereas NZEB renovations are rarely achieved. This paper proposes a methodology for the evaluation of renovation measures, aiming to provide decision support related to the selection of what to renovate and to what extent. The proposed method is rooted in the well-established cost-optimal methodology, yet it suggests a pre-step to package evaluation. This is done by means of a simplified cost-effective parameter (CEP), linking cost, lifetime, and energy savings. The methodology is demonstrated using a case study building in Denmark. The results show that the CEP provides good grounds for the compilation of single actions to packages. Further developments could focus on the sensitivity of the model inputs and integration of additional evaluation parameters to cost, such as environmental, architectural, comfort, risk, etc. [ABSTRACT FROM AUTHOR]

The aim of the study was to determine the effect of repeated hot thermal stress and cold water immersion on the endocrine system of young adult men with moderate and high levels of physical activity (PA). The research was conducted on 30 men aged 19-26 years (mean: 22.67 ± 2.02) who attended four sauna sessions of 12 min each (temperature: 90-91°C; relative humidity: 14-16 %). Each sauna session was followed by a 6-min cool-down break during which the participants were immersed in cold water (10-11°C) for 1 min. Testosterone (TES), cortisol (COR), dehydroepiandrosterone sulfate (DHEA-S), and prolactin (PRL) levels were measured before and after the sauna bath. The participants' PA levels were evaluated using the International Physical Activity Questionnaire. Serum COR levels decreased significantly (p < .001) from 13.61 to 9.67 μg/ml during 72 min of sauna treatment. No significant changes (p >.05) were noted in the concentrations of the remaining hormones: TES increased from 4.04 to 4.24 ng/ml, DHEA-S decreased from 357.5 to 356.82 μg/ml, and PRL decreased from 14.50 to 13.71 ng/ml. After sauna, a greater decrease in COR concentrations was observed in males with higher baseline COR levels, whereas only a minor decrease was noted in participants with very low baseline COR values (r =-0.673, p <.001). Repeated use of Finnish sauna induces a significant decrease in COR concentrations, but does not cause significant changes in TES, DHEA-S, or PRL levels. Testosterone concentrations were higher in men characterized by higher levels of PA, both before and after the sauna bath. [ABSTRACT FROM AUTHOR]

The present source publication is the fifth in a series encompassing the most important documents related to preparations for the replacement of French codes with national codification written by the Civil Reform Committee which worked between 1814 and 1815. Herein are contained the minutes of the sessions held by the Committee which concern a key debate among its members who argued as to whether to derogate the French Code of Civil Procedure in its entirety or only in fragments which were considered to be the most burdensome in Polish realities. Moreover, the publication also includes the first part of "The project of the civil judicial procedure in first instance" (primary procedure) presented by Franciszek Grabowski, a preeminent traditionalist in the Committee, along with fragments of the minutes reporting the course of the discussion concerning the project. Together with the second part of the proposition submitted by Grabowski encompassing enforcement proceedings, a counterproject of the code of civil procedure by progressive judge Antoni Bieńkowski, as well as a debate over these two proposals, which are to be published in the next issues of "Krakowskie Studia z Historii Państwa i Prawa", the materials presented below portray the crux of the debate held over the Code de procédure civile of 1806, which aroused much controversy, and which was in fact the most criticized part of the French legislation in force in Polish territories. [ABSTRACT FROM AUTHOR]

This study analysed carcass features and the chemical composition of semimembranosus (SM) and longissimus thoracis et lumborum (LTL) muscles from farmed-raised fallow deer (Dama dama L.) bucks (n = 8). Mineral contents and fatty acid composition were also determined in the muscles and the fulfilment of the demand for minerals was calculated for adults. Dressing percentage was 55.2%, whereas the proportions of round, shoulder and loin were 38.4%, 15.5% and 14.4%, respectively. The highest content of meat tissue was found in round, followed by loin. There were no differences in moisture, fat, protein or ash contents between SM and LTL muscles, however differences in mineral contents and fatty acid composition were noted and affected the concentration of nutrients. Meat from farmed-raised fallow deer is a good source of iron and copper in the human diet and may be recommended as a part of a healthy diet. [ABSTRACT FROM AUTHOR]

As part of the source publication series begun in the second fascicle of the twelfth volume of Krakowskie Studia z Historii Państwa i Prawa [Krakow Studies in History of State and Law], we are publishing this time documents related to the preparation of the Civil Reform Committee, which operated in 1814-1815, to develop a new national civil code to replace the Napoleonic Code: 1. Wstęp przy wprowadzeniu planu do kodeksu cywilnego [Introduction to the Plan of Implementation of the Civil Code], 2. Plan Bieńkowskiego do nowego kodeksu cywilnego [Bieńkowski's Plan for the New Civil Code], 3. Projekt Linowskiego do uformowania komisji mającej wygotować Księgę prawa cywilnego i procedury [Linowski's Plan for Forming a Commission to Prepare a Book of Civil Law and Procedure] and fragments of minutes of the Civil Reform Committee sessions regarding this draft. Source material on matrimonial law published in two last issues of the „Krakow Studies" in 2019 differed from the documents published in this fascicle because they were intended to serve only a partial reform of the Napoleonic Code envisaged for a temporary period, until a new civil code was developed. Antoni Bieńkowski presented his Plan for Implementation of the Civil Code at the session held on 20th November, 1814, less than a month after presenting the drafts of matrimonial law to the Civil Reform Committee (23rd October). The introductory part of the Plan presents the general assumptions regarding the works. They are followed by a list of the planned chapters and a justification of the adopted order. The actual Plan itself (second document) lists the same titles of chapters, along with the issues that should be regulated in a given place, and then it indicates where the models to follow could be sought. The debate related to the presentation of Bieńkowski's Plan and the scope of activities in this field entrusted to the Committee by Emperor Alexander, took place at sessions held from 17th to 24th November. It ended with the preparation of Aleksander Linowski's Plan for Forming a Commission to Prepare a Book of Civil Law and Procedure on 27th November. This document planned three stages of codification works: first, a 9-member commission was to draft both codes, and then departmental deputies were to work on them (first personally, and then representatives of them, with some Committee members). The last stage of preparatory works was to be comprised of meetings of five senators and members of the Council of State with selected Committee members and a representatives of departmental deputies. [ABSTRACT FROM AUTHOR]

The relations between the gentry and the peasantry in the Duchy of Warsaw period show their undoubtedly transitional character. The capitalistic civil law which was imposed by France did not harmonize at all with the partially feudal realities of the Polish countryside. Napoleon abolished serfdom in Article 4 of the Constitution. Soon after, Frederick Augustus I of Saxony issued the December Decree (1807). Its significance as well as consequences, despite the wealth of the literature concerning this subject matter, are extremely difficult to define even today. In the Polish People's Republic period, which was definitely a period of very intensive research into the issues of the exploitation of the peasants and their resistance in the feudal and bourgeoisie eras, it was claimed that the Decree abolished the shared ownership of farms and deprived the peasants of their rights (the abolition of dominium utile). These categorical conclusions were, as a rule, formulated without taking the court records into consideration. Furthermore, these records actually seem to indicate that the reality was much more complicated. The article delineates the disputes between the peasantry of two villages, Cienia and Michałów, and their landowners (or vice versa) before the Civil Tribunal of First Instance in Kalisz. It is worth mentioning that according to the peasants' awareness as well as to judge's opinions the December Decree did not put an end to the mutual rights and obligations between the gentry and the peasantry that had been shaped by customs and various documents which had been issued for centuries. [ABSTRACT FROM AUTHOR]

Purpose: The effect of open release of a post-traumatic elbow contracture on the stability of the joint has not been so far studied in vivo. Resection of elbow joint capsule, the key element of surgery, was reported to have no effect on the stability of cadaveric elbows. The joint capsule is yet known to participate in maintaining elbow stability as one of secondary stabilizers.Methods: We assessed elbow joint laxity in 39 patients who underwent an open contracture release via the 'column procedure' described by B. Morrey and P. Mansat within the preceeding three to nine months. The measurements were taken with an apparatus designed particularly for this experiment according to the predetermined protocol. A preliminary part of the experiment showed that there was no significant difference between laxity of two elbow joints in healthy volunteers. Laxity of the operated elbows could be then compared with the contralateral joints.Results: Mean absolute difference of laxity between healthy and operated elbows was 1.55° (0.1°-4.1°, SD = 1.1) being significantly lower than 2°, p = 0.0056. The difference of the joint laxity between the operated and healthy elbows did not differ statistically significantly by more than 0.6° from the difference of the laxity of two healthy elbows and, therefore, is not clinically noticeable.Conclusions: Our experiment confirmed that the 'column procedure' is a safe procedure which does not compromise the stability of the elbow joint. [ABSTRACT FROM AUTHOR]

In canine and feline populations, the number of neoplasm cases continues to increase around the world. Attempts are being made in centres of research to identify new biomarkers that speed up and improve the quality of oncological diagnostics and therapy in human and animal tumour patients. Cyclooxygenase-2 (COX-2) is a promising biomarker with increasing relevance to human oncology, but as yet with less application in veterinary oncology. The expression of COX-2 increases significantly during pathological processes involving inflammation, pain or fever. It is also overexpressed in humans presenting various types of tumours and in selected types of tumours in animals, particularly in dogs. This article discusses the expression of COX-2 in canine and feline tumours, the importance of COX-2 as a biomarker with diagnostic, therapeutic, prognostic and predictive relevance in oncology, and the clinical significance of inhibiting COX-2 overexpression in tumours. [ABSTRACT FROM AUTHOR]

The article provides information on the number of divorce judgements in Poland under Napoleonic Code until 1825 inlcuding final decree dissolving a marriage, marriage certificates and formulated sentences and sentence records. Topics include divorce courts functioning under the Napoleonic Code, marital breakdown and breakdown of interpersonal relationships.

The article describes the position of women pursuant to the codes adopted in the territories of contemporary Poland at the beginning of the nineteenth century (Allgemeines Landrecht für die Königlich Preussischen Staaten, Code civil des Français, Allgemeines Bürgerliches Gesetzbuch). According to all three codes wives were subordinate to their husbands, but there were many differences in specific matters. The husband usually administered common marriage property, or in case of separate property regime, he had a significant influence on his wife's property. In all the analysed codes, parental authority was exercised mainly by the father, although children had to obey their mother, too. The relations between the spouses could be fundamentally changed by prenuptial agreements. Moreover, the legal practice changed the letter of law many times and in numerous cases it differed between the territories where the same code was in force (e.g. France and the Congress Kingdom of Poland - in both countries the Napoleonic Code was in force). Thus, time-consuming research on sources like notarial deed, as well as court records should precede a complex analysis covering the application practice of all three codes on the Polish lands. [ABSTRACT FROM AUTHOR]

Purpose and Hypothesis: The aim of this study was to compare early clinical results after open and arthroscopic Latarjet stabilisation in anterior shoulder instability. Our hypothesis was the results of arthroscopic stabilisation were comparable with the results of open procedure.Material and Methods: The clinical results of the patients after primary Latarjet procedure were analysed. Patients operated on between 2006 and 2011 using an open technique composed the OPEN group and patients operated on arthroscopically between 2011 and 2013 composed the ARTHRO group; 48 out of 55 shoulders (87%) in OPEN and 62 out of 64 shoulders (97%) in ARTHRO were available to follow-up. The average age at surgery was 28 years in OPEN and 26 years in ARTHRO. The mean follow-up was 54.2 months in OPEN and 23.4 months in ARTHRO. Intra-operative data were analysed regarding time of surgery, concomitant lesions and complications. Patient results were assessed with Walch-Duplay, Rowe, VAS scores and subjective self-evaluation of satisfaction and shoulder function. Computed tomography scan evaluation was used to assess the graft healing.Results: Average time of surgery was significantly shorter in ARTHRO than OPEN: respectively 110 and 120 minutes. The number of intra-operative complications was six (12.5%) in OPEN and five (8.1%) in ARTHRO. The results were comparable in both groups, with no significant difference between OPEN and ARTHRO group: satisfaction rate - 96.8% and 91.9%, shoulder function - 92.2% and 90%, Walch-Duplay score - 83.9 and 76.7 respecively. A significant difference was reported in Rowe score: 87.8 in OPEN and 78.9 in ARTHRO. Another significant difference was found in the presence of "subjective apprehension"-a term referring to the subjective perception of instability with no signs of instability at clinical examination - 28.7% in OPEN and 50% in ARTHRO. Range of motion in both groups were comparable, however patients in OPEN had significantly lower loss of external rotation in adduction to the side comparing to the contralateral shoulder: 7° versus 14° in ARTHRO. Recurrence was reported in three cases in each group: 6.2% in OPEN and 4.8% in ARTHRO. A revision surgery was performed in four patients (9.3%) in OPEN and six (9.7%) in ARTHRO. Radiographic evaluation showed a significantly lower rate (5%) of graft healing problems (fracture, non-union and osteolysis) after arthroscopic stabilisation, however a partial osteolysis of the proximal part of the bone block was significantly more frequent (53.5%).Conclusions: The arthroscopic Latarjet stabilisation showed satisfactory and comparable results to open procedure. We recommend further investigation and development of arthroscopic technique.Level Of Evidence: III. [ABSTRACT FROM AUTHOR]

We present three source texts. The first are the writings of father Dhiel, General Superintendent of the Reformed Evangelical Congregations, which dealt with matrimonial laws and divorces. Next is the draft act by A. Bieńkowski concerning property contracts between spouses, and the last are fragments of Civil Reform Committee session minutes regarding these writings. The author of the first text covered three issues of fundamental significance to the Protestants, those being: obstacles to contracting marriage, premises for divorce, and the problems of jurisdiction in divorce cases. The author of the published draft act, in turn, postulated the reinstatement of the separation of assets, which had functioned previously under ius terrestre as the statutory property regime in marriage. This solution was in direct opposition to the one introduced in the Napoleonic Code. The draft act was much shorter than the chapter of the Napoleonic Code that it was to replace: 34 articles as compared to 195. In many aspects it could be deemed fragmentary, written from the perspective of the landed gentry, and omitting many problems that concerned other social strata. [ABSTRACT FROM AUTHOR]

Purpose: The goal of this study was to evaluate clinical and radiological outcomes after arthroscopic Latarjet stabilisation in anterior shoulder instability.Methods: Ninety-three patients after primary arthroscopic Latarjet stabilisation were reviewed. Satisfaction, subjective shoulder value (SSV), Walch-Duplay and Rowe scores, and range of motion and stability were evaluated on clinical examination. Computed tomography (CT) was used to analyse graft position and fusion.Results: Ninety patients (96.8%) were available for clinical and 85 for CT evaluation. The mean follow-up was 23.7 months (13-50, SD 7.1) and age at surgery was 26.2 years (16-44, SD 5.6). Intraoperative complications were reported in eight patients (8.9%) and recurrence in three (3.3%). Significantly, two out of three patients with recurrence had intraoperative graft complications (p = 0.0107). Forty-one patients (45.6%) reported the feeling of "subjective return to sport anxiety". External rotation with arm at the side was 59° (10-90°, SD 20) with 15° (0-70°, SD 17) of loss of rotation. These two factors correlated with results the most. Patient satisfaction was evaluated as 92% (40-100, SD 14) and SSV 90% (30-100, SD12). Revision rate after primary surgery was 10%. CT showed graft healing in 81 (95.3%) patients. A graft position between 2 and 5 o'clock was found in 70 (83.4%) patients and flush to the anterior glenoid rim in 34 (40.5%). Osteolysis of the superior part of the graft was found in 55 (64.7%) patients. CT evaluation showed no correlation with clinical results.Conclusion: Arthroscopic Latarjet stabilisation demonstrates satisfactory results in short-term follow-up; however, intraoperative graft-related complications are a risk factor for recurrence. "Subjective return to sport anxiety" and loss of external rotation with the arm at the side are factors worsening the results. Graft position imperfections and osteolysis of the superior part of the graft reported in CT evaluation do not influence the clinical results. [ABSTRACT FROM AUTHOR]

Summary: Growing interest of Arctic char (Salvelinus alpinus) aquaculture in Europe, and the fact that it can easily hybridize with brook trout (Salvelinus fontinalis) resulting in fertile progeny, led us to investigate fish from the farmed stocks. Chromosomes of sampled Arctic char were examined using conventional and molecular cytogenetic (FISH) techniques in order to determine possible contamination of genomic elements of brook trout. Investigated fish possessed karyotypes composed of 80–82 chromosomes and up to three chromosome fragments. Using staining methods and FISH approach enabled identification of the brook trout chromosomes in the eight out of twenty‐two examined Arctic char. Specific location of AT‐, GC‐ positive and NOR sites observed on chromosomes as well as chromosome fragments in the karyotypes of several individuals points on past chromosomal rearrangements in fish from examined broodstock. Based on our results, it may be assumed that individuals with the brook trout genomic elements, although phenotypically identified as Arctic chars, were hybrids. Our results highlights that special care should be taken to protect gene pools of brook trout and Arctic char in farms where both species are cultured. [ABSTRACT FROM AUTHOR]

As part of the series of source publications commenced in the second fascicule of the 12th volume of "Krakowskie Studia z Historii Państwa i Prawa" [Krakow Studies in History of State and Law], we are publishing [Draft] to Replace Title 5 of Book 1 of the French Civil Code and fragments of the minutes of Civil Reform Committee's sessions concerning this draft. On 23 October of 1814 Antoni Bieńkowski presented at the Committee's session drafts of marital laws: personal and property. The former of the two drafts is published below, whereas the latter will be printed in the upcoming fascicule of "Krakowskie Studia z Historii Państwa i Prawa". This draft, similarly to the others penned by Committee members, never came into effect as law. The issue of upholding the lay system of marital law, introduced by the Napoleonic Code, remained a contentious one for years to come. In 1825 the Sejm enacted Book One of the Civil Code of the Kingdom of Poland, which implemented a mixed model of marital personal law. Lay elements, however, were a lot stronger there than in the 1814 draft (particularly, also marital issues concerning Catholics were placed within the jurisdiction of common courts of law). Also this law was heavily criticized by the conservative circles. [ABSTRACT FROM AUTHOR]

Bisphenol A, a substance commonly used in plastic manufacturing, is relatively well known as an endocrine disruptor, which may bind to estrogen receptors and has multidirectional negative effects on both human and animal organisms. Previous studies have reported that BPA may act on the reproductive organs, but knowledge concerning BPA-induced changes within the nerves located in the uterine wall is extremely scant. The aim of this study was to investigate the impact of various doses of BPA on the parasympathetic nerves located in the corpus and horns of the uterus using a single and double immunofluorescence method. The obtained results have shown that BPA may change not only the expression of vesicular acetylcholine transporter (VAChT—a marker of parasympathetic nervous structures) in the uterine intramural nerve fibers, but also the degree of colocalization of this substance with other neuronal factors, including substance P (SP), vasoactive intestinal polypeptide (VIP), galanin (GAL), and calcitonin gene–related peptide (CGRP). Moreover, BPA caused changes in the density of the overall populations of fibers immunoreactive to the particular neuropeptides mentioned above. The characteristics of the changes clearly depended on the part of the uterus, the neuronal factors studied, and the dosage of BPA. The mechanisms of the observed fluctuations are probably connected with the neurotoxic and/or pro-inflammatory activity of BPA. Moreover, the results have shown that even low doses of BPA are not neutral to living organisms. Changes in the neurochemical characterization of nerves supplying the uterine wall may be the first subclinical sign of intoxication with this substance. [ABSTRACT FROM AUTHOR]