21 results on '"Park, Hee Ra"'
Search Results
2. Novel Psychopharmacological Herbs Relieve Behavioral Abnormalities and Hippocampal Dysfunctions in an Animal Model of Post-Traumatic Stress Disorder.
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Park, Hee Ra, Cai, Mudan, and Yang, Eun Jin
- Abstract
Post-traumatic stress disorder (PTSD) is an anxiety disorder caused by traumatic or frightening events, with intensified anxiety, fear memories, and cognitive impairment caused by a dysfunctional hippocampus. Owing to its complex phenotype, currently prescribed treatments for PTSD are limited. This study investigated the psychopharmacological effects of novel COMBINATION herbal medicines on the hippocampus of a PTSD murine model induced by combining single prolonged stress (SPS) and foot shock (FS). We designed a novel herbal formula extract (HFE) from Chaenomeles sinensis, Glycyrrhiza uralensis, and Atractylodes macrocephala. SPS+FS mice were administered HFE (500 and 1000 mg/kg) once daily for 14 days. The effects of HFE of HFE on the hippocampus were analyzed using behavioral tests, immunostaining, Golgi staining, and Western blotting. HFE alleviated anxiety-like behavior and fear response, improved short-term memory, and restored hippocampal dysfunction, including hippocampal neurogenesis alteration and aberrant migration and hyperactivation of dentate granule cells in SPS+FS mice. HFE increased phosphorylation of the Kv4.2 potassium channel, extracellular signal-regulated kinase, and cAMP response element-binding protein, which were reduced in the hippocampus of SPS+FS mice. Therefore, our study suggests HFE as a potential therapeutic drug for PTSD by improving behavioral impairment and hippocampal dysfunction and regulating Kv4.2 potassium channel-related pathways in the hippocampus. [ABSTRACT FROM AUTHOR]
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- 2023
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3. Low-dose curcumin enhances hippocampal neurogenesis and memory retention in young mice.
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Lee, Yujeong, Park, Hee Ra, Lee, Joo Yeon, Kim, Jaehoon, Yang, Seonguk, Lee, Chany, Kim, Kipom, Kim, Hyung Sik, Chang, Seung-Cheol, and Lee, Jaewon
- Abstract
Adult neurogenesis generates new functional neurons from adult neural stem cells in various regions, including the subventricular zone (SVZ) of the lateral ventricles and subgranular zone (SGZ) of hippocampal dentate gyrus (DG). Available evidence shows hippocampal neurogenesis can be negatively or positively regulated by dietary components. In a previous study, we reported that curcumin (diferuloylmethane; a polyphenolic found in curry spice) stimulates the proliferation of embryonic neural stem cells (NSCs) by activating adaptive cellular stress responses. Here, we investigated whether subchronic administration of curcumin (once daily at 0.4, 2, or 10 mg/kg for 14 days) promotes hippocampal neurogenesis and neurocognitive function in young (5-week-old) mice. Oral administration of low-dose curcumin (0.4 mg/kg) increased the proliferation and survival of newly generated cells in hippocampus, but surprisingly, high-dose curcumin (10 mg/kg) did not effectively upregulate the proliferation or survival of newborn cells. Furthermore, hippocampal BDNF levels and phosphorylated CREB activity were elevated in only low-dose curcumin-treated mice. Passive avoidance testing revealed that low-dose curcumin increased cross-over latency times, indicating enhanced memory retention, and an in vitro study showed that low-concentration curcumin increased the proliferative activity of neural progenitor cells (NPCs) by upregulating NF1X levels. Collectively, our findings suggest that low-dose curcumin has neurogenic effects and that it may prevent age and neurodegenerative disease-related cognitive deficits. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Nutraceutical Interventions for Post-Traumatic Stress Disorder in Animal Models: A Focus on the Hypothalamic–Pituitary–Adrenal Axis.
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Cai, Mudan, Park, Hee Ra, and Yang, Eun Jin
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POST-traumatic stress disorder ,HYPOTHALAMIC-pituitary-adrenal axis ,EFFECT of stress on animals ,SEROTONIN uptake inhibitors ,MENTAL depression ,SOCIAL anxiety ,ANXIETY - Abstract
Post-traumatic stress disorder (PTSD) occurs after exposure to traumatic events and is characterized by overwhelming fear and anxiety. Disturbances in the hypothalamic–pituitary–adrenal (HPA) axis are involved in the pathogenesis of mood disorders, including anxiety, PTSD, and major depressive disorders. Studies have demonstrated the relationship between the HPA axis response and stress vulnerability, indicating that the HPA axis regulates the immune system, fear memory, and neurotransmission. The selective serotonin reuptake inhibitors (SSRIs), sertraline and paroxetine, are the only drugs that have been approved by the United States Food and Drug Administration for the treatment of PTSD. However, SSRIs require long treatment times and are associated with lower response and remission rates; therefore, additional pharmacological interventions are required. Complementary and alternative medicine therapies ameliorate HPA axis disturbances through regulation of gut dysbiosis, insomnia, chronic stress, and depression. We have described the cellular and molecular mechanisms through which the HPA axis is involved in PTSD pathogenesis and have evaluated the potential of herbal medicines for PTSD treatment. Herbal medicines could comprise a good therapeutic strategy for HPA axis regulation and can simultaneously improve PTSD-related symptoms. Finally, herbal medicines may lead to novel biologically driven approaches for the treatment and prevention of PTSD. [ABSTRACT FROM AUTHOR]
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- 2022
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5. Mitochondrial genome mutations and neuronal dysfunction of induced pluripotent stem cells derived from patients with Alzheimer's disease.
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Lee, Yeonmi, Kim, Minchul, Lee, Miju, So, Seongjun, Kang, Soon‐Suk, Choi, Jiwan, Kim, Deokhoon, Heo, Hyohoon, Lee, Sung Soo, Park, Hee Ra, Ko, Jung Jae, Song, Jihwan, and Kang, Eunju
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INDUCED pluripotent stem cells ,MITOCHONDRIAL DNA ,PLURIPOTENT stem cells ,ALZHEIMER'S patients ,CORD blood ,NEURONAL differentiation - Abstract
Objectives: Patient‐derived induced pluripotent stem cells (iPSCs) are materials that can be used for autologous stem cell therapy. We screened mtDNA mutations in iPSCs and iPSC‐derived neuronal cells from patients with Alzheimer's disease (AD). Also, we investigated whether the mutations could affect mitochondrial function and deposition of β‐amyloid (Aβ) in differentiated neuronal cells. Materials and Methods: mtDNA mutations were measured and compared among iPSCs and iPSC‐derived neuronal cells. The selected iPSCs carrying mtDNA mutations were subcloned, and then their growth rate and neuronal differentiation pattern were analyzed. The differentiated cells were measured for mitochondrial respiration and membrane potential, as well as deposition of Aβ. Results: Most iPSCs from subjects with AD harbored ≥1 mtDNA mutations, and the number of mutations was significantly higher than that from umbilical cord blood. About 35% and 40% of mutations in iPSCs were shared with isogenic iPSCs and their differentiated neuronal precursor cells, respectively, with similar or different heteroplasmy. Furthermore, the mutations in clonal iPSCs were stable during extended culture and neuronal differentiation. Finally, mtDNA mutations could induce a growth advantage with higher viability and proliferation, lower mitochondrial respiration and membrane potential, as well as increased Aβ deposition. Conclusion: This study demonstrates that mtDNA mutations in patients with AD could lead to mitochondrial dysfunction and accelerated Aβ deposition. Therefore, early screening for mtDNA mutations in iPSC lines would be essential for developing autologous cell therapy or drug screening for patients with AD. [ABSTRACT FROM AUTHOR]
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- 2022
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6. Neurogenic Interventions for Fear Memory via Modulation of the Hippocampal Function and Neural Circuits.
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Park, Hee Ra, Cai, Mudan, and Yang, Eun Jin
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NEURAL circuitry ,AMYGDALOID body ,POST-traumatic stress disorder ,HIPPOCAMPUS (Brain) ,ANIMAL memory ,PREFRONTAL cortex - Abstract
Fear memory helps animals and humans avoid harm from certain stimuli and coordinate adaptive behavior. However, excessive consolidation of fear memory, caused by the dysfunction of cellular mechanisms and neural circuits in the brain, is responsible for post-traumatic stress disorder and anxiety-related disorders. Dysregulation of specific brain regions and neural circuits, particularly the hippocampus, amygdala, and medial prefrontal cortex, have been demonstrated in patients with these disorders. These regions are involved in learning, memory, consolidation, and extinction. These are also the brain regions where new neurons are generated and are crucial for memory formation and integration. Therefore, these three brain regions and neural circuits have contributed greatly to studies on neural plasticity and structural remodeling in patients with psychiatric disorders. In this review, we provide an understanding of fear memory and its underlying cellular mechanisms and describe how neural circuits are involved in fear memory. Additionally, we discuss therapeutic interventions for these disorders based on their proneurogenic efficacy and the neural circuits involved in fear memory. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Neural stem cells derived from the developing forebrain of YAC128 mice exhibit pathological features of Huntington's disease.
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Li, Endan, Park, Hee Ra, Hong, Chang Pyo, Kim, Younghoon, Choi, Jiwoo, Lee, Suji, Park, Hyun Jung, Lee, Bomi, Kim, Tae Aug, Kim, Seong Jin, Kim, Hyun Sook, and Song, Jihwan
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NEURAL stem cells ,HUNTINGTON disease ,ARTIFICIAL chromosomes ,TRANSGENIC mice ,PROSENCEPHALON ,AXONAL transport - Abstract
Objectives: Huntington's disease (HD) is a devastating neurodegenerative disease caused by polyglutamine (polyQ) expansion in the huntingtin (HTT) gene. Mutant huntingtin (mHTT) is the main cause of HD and is associated with impaired mitochondrial dynamics, ubiquitin‐proteasome system and autophagy, as well as tauopathy. In this study, we aimed to establish a new neural stem cell line for HD studies. Materials and methods: YAC128 mice are a yeast artificial chromosome (YAC)‐based transgenic mouse model of HD. These mice express a full‐length human mutant HTT gene with 128 CAG repeats and exhibit various pathophysiological features of HD. In this study, we isolated a new neural stem cell line from the forebrains of YAC128 mouse embryos (E12.5) and analysed its characteristics using cellular and biochemical methods. Results: Compared to wild‐type (WT) NSCs, the YAC128 NSC line exhibited greater proliferation and migration capacity. In addition to mHTT expression, increased intracellular Ca2+ levels and dysfunctional mitochondrial membrane potential were observed in the YAC128 NSCs. YAC128 NSCs had defects in mitochondrial dynamics, including a deficit in mitochondrial axonal transport and unbalanced fusion and fission processes. YAC128 NSCs also displayed decreased voltage response variability and Na+ current amplitude. Additionally, the ubiquitin‐proteasome and autophagy systems were impaired in the YAC128 NSCs. Conclusions: We have established a new neural stem line from YAC128 transgenic mice, which may serve as a useful resource for studying HD pathogenesis and drug screening. [ABSTRACT FROM AUTHOR]
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- 2020
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8. Enhancement of neuroprotective activity of Sagunja-tang by fermentation with lactobacillus strains.
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Yim, Nam-Hui, Gu, Min Jung, Park, Hee Ra, Hwang, Youn-Hwan, and Ma, Jin Yeul
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REACTIVE oxygen species ,BIOLOGICAL transport ,CELL death ,CELL lines ,ETOPOSIDE ,FERMENTATION ,GLYCOSIDES ,HERBAL medicine ,HIGH performance liquid chromatography ,HYDROGEN peroxide ,LACTOBACILLUS ,LIQUID chromatography ,MASS spectrometry ,CHINESE medicine ,NERVOUS system ,NEUROBLASTOMA ,TERPENES ,PHYTOCHEMICALS ,OXIDATIVE stress - Abstract
Background: Sagunja-tang (SGT) is widely used in traditional herbal medicine to treat immune system and gastrointestinal disorders and reportedly has protective effects against inflammation, cancer, and osteoporosis. In this study, we fermented SGT with different Latobacillus strains and investigated the change in phytochemical compositions in SGT and enhancement of it neuroprotective effects in SH-SY5Y human neuroblastoma. Methods: Marker components, including ginsenoside Rg
1 , glycyrrhizin, liquiritin, liquiritigenin, atractylenolide I, atractylenolide II, atractylenolide III, and pachymic acid, in SGT, were qualitatively and quantitatively analyzed using high-performance liquid chromatography–diode array detection and liquid chromatography–mass spectrometry. SGT was fermented with eight different Lactobacillus strains to yield eight fermented SGTs (FSGTs). The conversion efficiencies of SGT marker components were determined in each FSGT. To detect the protective effect of SGT and FSGT, reactive oxygen species (ROS) assay and mitochondrial membrane potentials (MMPs) assay were performed in SH-SY5Y cells. Results: Compared with the other FSGTs, SGT166, i.e., SGT fermented with L. plantarum 166, had high conversion efficiency, as indicated by increased amounts of glycyrrhizin, liquiritigenin, and atractylenolides I–III. In SH-SY5Y cells, protection against cell death induced by H2 O2 and etoposide was high using SGT166 and very low using SGT. Furthermore, ROS production and mitochondrial membrane potential disruption in SH-SY5Y cells were markedly suppressed by SGT166 treatment, which demonstrated that inhibition of ROS generation may be one of the neuroprotective mechanisms of SGT166. Conclusions: This study demonstrated that fermentation of SGT with L. plantarum 166 enhanced suppression of oxidative stress and MMP loss. This enhanced neuroprotective effect was thought to be caused by the conversion of SGT phytochemicals by fermentation. SGT166 shows potential for treating neurological damage-related diseases. [ABSTRACT FROM AUTHOR]- Published
- 2018
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9. Protective Effects of Spatholobi Caulis Extract on Neuronal Damage and Focal Ischemic Stroke/Reperfusion Injury.
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Park, Hee Ra, Lee, Heeeun, Lee, Jung-Jin, Yim, Nam-Hui, Gu, Min-Jung, and Ma, Jin Yeul
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Neuronal apoptotic cell death plays an important role in many neurological disorders, including Alzheimer’s disease, Parkinson’s disease, and ischemic stroke. Spatholobi Caulis (SC) has been widely used in traditional herbal medicine for the treatment of cancer, inflammation, viral infection, and anemia. However, the protective effects of SC extract (SCE) against apoptotic cell death in the brain have not been reported. We investigated the protective effects of SCE against neuronal injury etoposide-induced neurotoxicity and in rats subjected to focal transient ischemic stroke middle cerebral artery occlusion (MCAO) for 45 min, followed by 7 days of reperfusion. The in vitro study demonstrated that SCE protected cells against etoposide-induced cell viability loss in SH-SY5Y cells. Apoptotic phenotypes, such as cleaved PARP and caspase-3, and oxidative stress in etoposide-treated cells were ameliorated by SCE treatment. In MCAO-reperfusion injury, SCE promoted neuronal survival and level of brain-derived neurotrophic factor (BDNF) by reducing glial activation, oxidative stress, and apoptosis in the ipsilateral cortex. These results indicated that SCE exerted protective effects under etoposide treatment and in a MCAO-reperfusion model by reducing JNK and p38 MAPK activation. This study presents the first evidence that SCE has therapeutic potential for the treatment of ischemic stroke or neurological disorder-related cell death. [ABSTRACT FROM AUTHOR]
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- 2018
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10. Learning, memory deficits, and impaired neuronal maturation attributed to acrylamide.
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Lee, Seulah, Park, Hee Ra, Lee, Joo Yeon, Cho, Jung-Hyun, Song, Hye Min, Kim, Ah Hyun, Lee, Wonjong, Lee, Yujeong, Chang, Seung-Cheol, Kim, Hyung Sik, and Lee, Jaewon
- Subjects
ACRYLAMIDE ,SKELETAL muscle ,NEUROTOXICOLOGY - Abstract
Acrylamide (ACR) is a neurotoxin known to produce neurotoxicity characterized by ataxia, skeletal muscle weakness, cognitive impairment, and numbness of the extremities. Previously, investigators reported that high-dose (50 mg/kg) ACR impaired hippocampal neurogenesis and increased neural progenitor cell death; however, the influence of subchronic environmentally relevant low dose-(2, 20, or 200 μg/kg) ACRs have not been examined in adult neurogenesis or cognitive function in mice. Accordingly, the aim of the present study was to investigate whether low-dose ACR adversely affected mouse hippocampal neurogenesis and neurocognitive functions. Male C57BL/6 mice were orally administered vehicle or ACR at 2, 20, or 200 μg/kg/day for 4 weeks. ACR did not significantly alter the number of newly generated cells or produce neuroinflammation or neuronal loss in hippocampi. However, behavioral studies revealed that 200 μg/kg ACR produced learning and memory impairment. Furthermore, incubation of ACR with primary cultured neurons during the developmental stage was found to delay neuronal maturation without affecting cell viability indicating the presence of developmental neurotoxicity. These findings indicate that although exposure to
in vivo low-dose ACR daily for 4 weeks exerted no apparent marked effect on hippocampal neurogenesis,in vitro observations in primary cultured neurons noted adverse effects on learning and memory impairment suggestive of neurotoxic actions. [ABSTRACT FROM AUTHOR]- Published
- 2018
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11. The mitochondrial uncoupler DNP triggers brain cell mTOR signaling network reprogramming and CREB pathway up-regulation.
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Liu, Dong, Zhang, Yongqing, Gharavi, Robert, Park, Hee Ra, Lee, Jaewon, Siddiqui, Sana, Telljohann, Richard, Nassar, Matthew R., Cutler, Roy G., Becker, Kevin G., and Mattson, Mark P.
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CELLULAR signal transduction ,MTOR protein ,NEURAL circuitry ,BRAIN-derived neurotrophic factor ,AUTOPHAGY ,DINITROPHENOL ,CALORIC expenditure ,THERAPEUTICS - Abstract
Mitochondrial metabolism is highly responsive to nutrient availability and ongoing activity in neuronal circuits. The molecular mechanisms by which brain cells respond to an increase in cellular energy expenditure are largely unknown. Mild mitochondrial uncoupling enhances cellular energy expenditure in mitochondria and can be induced with 2,4-dinitrophenol ( DNP), a proton ionophore previously used for weight loss. We found that DNP treatment reduces mitochondrial membrane potential, increases intracellular Ca
2+ levels and reduces oxidative stress in cerebral cortical neurons. Gene expression profiling of the cerebral cortex of DNP-treated mice revealed reprogramming of signaling cascades that included suppression of the mammalian target of rapamycin ( mTOR) and insulin - PI3K - MAPK pathways, and up-regulation of tuberous sclerosis complex 2, a negative regulator of mTOR. Genes encoding proteins involved in autophagy processes were up-regulated in response to DNP. CREB ( cAMP-response element-binding protein) signaling, Arc and brain-derived neurotrophic factor, which play important roles in synaptic plasticity and adaptive cellular stress responses, were up-regulated in response to DNP, and DNP-treated mice exhibited improved performance in a test of learning and memory. Immunoblot analysis verified that key DNP-induced changes in gene expression resulted in corresponding changes at the protein level. Our findings suggest that mild mitochondrial uncoupling triggers an integrated signaling response in brain cells characterized by reprogramming of mTOR and insulin signaling, and up-regulation of pathways involved in adaptive stress responses, molecular waste disposal, and synaptic plasticity. [ABSTRACT FROM AUTHOR]- Published
- 2015
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12. Silibinin prevents dopaminergic neuronal loss in a mouse model of Parkinson's disease via mitochondrial stabilization.
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Lee, Yujeong, Park, Hee Ra, Chun, Hye Jeong, and Lee, Jaewon
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- 2015
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13. Baicalein attenuates astroglial activation in the 1-methyl-4-phenyl-1,2,3,4-tetrahydropyridine-induced Parkinson's disease model by downregulating the activations of nuclear factor-κB, ERK, and JNK.
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Lee, Eunjin, Park, Hee Ra, Ji, Seung Taek, Lee, Yujeong, and Lee, Jaewon
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- 2014
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14. Baicalein attenuates impaired hippocampal neurogenesis and the neurocognitive deficits induced by γ-ray radiation.
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Oh SB, Park HR, Jang YJ, Choi SY, Son TG, Lee J, Oh, Shin Bi, Park, Hee Ra, Jang, Young Jung, Choi, Seon Young, Son, Tae Gen, and Lee, Jaewon
- Abstract
Background and Purpose: Whole-brain irradiation (WBI) therapy produces learning and memory deficits in patients with brain tumours. Although the pathological cascade of cognitive deficits remains unknown, it may involve reduced neurogenesis within the hippocampus. Baicalein is a flavonoid derived from the roots of Huangqin, Scutellaria baicalensis Georgi, and has been shown to have antioxidant effects. Here, we have investigated the protective effects of baicalein on irradiation-induced impairments in hippocampal neurogenesis and cognitive function.Experimental Approach: Radioprotective effects of baicalein were evaluated in C17.2 neural progenitor cells and 6-week-old male C57BL/6 mice during hippocampal neurogenesis. Mice were given a single dose of 5 Gy WBI. Changes in hippocampal neurogenesis, oxidative stress and BDNF-pCREB signalling were evaluated. Morris water maze and passive avoidance test were used to assess learning and memory.Key Results: Baicalein protected neural progenitor cells against irradiation-induced necrotic cell death. Pretreatment with baicalein attenuated the irradiation-induced impairment of hippocampal neurogenesis by modulating oxidative stress and elevating BDNF-pCREB signalling. Furthermore, baicalein prevented the spatial learning and memory retention deficits follwing WBI.Conclusions and Implications: Our findings suggest that baicalein can be viewed as a potential therapeutic agent that protects against the impaired neurogenesis induced by WBI, and its neurocognitive consequences. [ABSTRACT FROM AUTHOR]- Published
- 2013
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15. Baicalein attenuates impaired hippocampal neurogenesis and the neurocognitive deficits induced by γ-ray radiation.
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Oh, Shin Bi, Park, Hee Ra, Jang, Young Jung, Choi, Seon Young, Son, Tae Gen, and Lee, Jaewon
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HIPPOCAMPUS (Brain) ,DEVELOPMENTAL neurobiology ,TOTAL body irradiation ,LEARNING disabilities ,COGNITION disorders ,BRAIN tumors ,PATIENTS - Abstract
Background and Purpose Whole-brain irradiation ( WBI) therapy produces learning and memory deficits in patients with brain tumours. Although the pathological cascade of cognitive deficits remains unknown, it may involve reduced neurogenesis within the hippocampus. Baicalein is a flavonoid derived from the roots of Huangqin, Scutellaria baicalensis Georgi, and has been shown to have antioxidant effects. Here, we have investigated the protective effects of baicalein on irradiation-induced impairments in hippocampal neurogenesis and cognitive function. Experimental Approach Radioprotective effects of baicalein were evaluated in C17.2 neural progenitor cells and 6-week-old male C57 BL/6 mice during hippocampal neurogenesis. Mice were given a single dose of 5 Gy WBI. Changes in hippocampal neurogenesis, oxidative stress and BDNF- pCREB signalling were evaluated. Morris water maze and passive avoidance test were used to assess learning and memory. Key Results Baicalein protected neural progenitor cells against irradiation-induced necrotic cell death. Pretreatment with baicalein attenuated the irradiation-induced impairment of hippocampal neurogenesis by modulating oxidative stress and elevating BDNF- pCREB signalling. Furthermore, baicalein prevented the spatial learning and memory retention deficits follwing WBI. Conclusions and Implications Our findings suggest that baicalein can be viewed as a potential therapeutic agent that protects against the impaired neurogenesis induced by WBI, and its neurocognitive consequences. [ABSTRACT FROM AUTHOR]
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- 2013
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16. Naphthazarin has a protective effect on the 1-methyl-4-phenyl-1,2,3,4-tetrahydropyridine-induced Parkinson's disease model.
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Choi, Seon Young, Son, Tae Gen, Park, Hee Ra, Jang, Young Jung, Oh, Shin Bi, Jin, Bora, and Lee, Jaewon
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- 2012
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17. The hepatoprotective effects of adenine nucleotide translocator-2 against aging and oxidative stress.
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Kim, Hyun Soo, Je, Jeong Hwan, Son, Tae Gen, Park, Hee Ra, Ji, Seung Taek, Pokharel, Yuba Raj, Jeon, Hyun Min, Kang, Keon Wook, Kang, Ho Sung, Chang, Seung-Cheol, Kim, Hyung Sik, Chung, Hae Young, and Lee, Jaewon
- Subjects
ADENINE nucleotide translocase ,OXIDATIVE stress ,FIBROSIS ,AGING ,MITOCHONDRIAL membranes ,GENE expression ,ADENOSINE triphosphate ,BIOENERGETICS - Abstract
Mitochondrial adenine nucleotide translocator (ANT) plays important roles in the regulation of mitochondrial permeability transition and cell bioenergetics. The mouse has three ANT isoforms (1, 2 and 4) showing tissue-specific expression patterns. Although ANT1 is known to have a pro-apoptotic property, the specific functions of ANT2 have not been well determined. In the present study, ANT2 expression was significantly lower in the aged rat liver and in a liver fibrosis model. To explore the protective role of ANT2 in the liver, we established a hepa1c1c7 cell line overexpressing ANT2. Overexpression of ANT2 caused hepa1c1c7 cells to be more resistant to oxidative stress, and mitochondrial membrane potential (MMP, ∆Ψm) was relatively intact in ANT2-overexpressing cells under oxidative stress. In addition, ANT2 was found to increase ATP production by influencing mitochondrial bioenergetics. These results imply that the hepatoprotective effect of ANT2 is due to the stabilization of MMP and enhanced ATP production, and thus, maintaining ANT2 levels in the liver might be important to enhance resistance to aging and oxidative stress. [ABSTRACT FROM AUTHOR]
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- 2012
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18. Neurogenic contributions made by dietary regulation to hippocampal neurogenesis.
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Park, Hee Ra and Lee, Jaewon
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HIPPOCAMPUS (Brain) ,DEVELOPMENTAL neurobiology ,LOW-calorie diet ,NEURAL stem cells ,DENTATE gyrus ,PHYSICAL activity ,FUNCTIONAL foods - Abstract
Adult neural stem cells in the dentate gyrus of the hippocampus are negatively and positively regulated by a broad range of environmental stimuli that include aging, stress, social interaction, physical activity, and dietary modulation. Interestingly, dietary regulation has a distinct outcome, such that reduced dietary intake enhances neurogenesis, whereas excess calorie intake by a high-fat diet has a negative effect. As a type of metabolic stress, dietary restriction (DR) is also known to extend life span and increase resistance to age-related neurodegenerative diseases. However, the potential application of DR as a 'neurogenic enhancer' in humans remains problematic because of the severity of restriction and the protracted duration of the treatment required. Therefore, the authors consider that an understanding of the neurogenic mechanisms of DR would provide a basis for the identification of the pharmacological and nutraceutical interventions that mimic the beneficial effects of DR without limiting caloric intake. The current review describes the regulatory effect of DR on hippocampal neurogenesis and presents a possible neurogenic mechanism. [ABSTRACT FROM AUTHOR]
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- 2011
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19. Oxidative Stress as a Therapeutic Target in Amyotrophic Lateral Sclerosis: Opportunities and Limitations.
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Park, Hee Ra and Yang, Eun Jin
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AMYOTROPHIC lateral sclerosis ,OXIDATIVE stress ,MOTOR neuron diseases ,MOTOR neurons ,SPINAL cord ,SPINAL cord injuries - Abstract
Amyotrophic lateral sclerosis (ALS), also known as motor neuron disease (MND) and Lou Gehrig's disease, is characterized by a loss of the lower motor neurons in the spinal cord and the upper motor neurons in the cerebral cortex. Due to the complex and multifactorial nature of the various risk factors and mechanisms that are related to motor neuronal degeneration, the pathological mechanisms of ALS are not fully understood. Oxidative stress is one of the known causes of ALS pathogenesis. This has been observed in patients as well as in cellular and animal models, and is known to induce mitochondrial dysfunction and the loss of motor neurons. Numerous therapeutic agents have been developed to inhibit oxidative stress and neuroinflammation. In this review, we describe the role of oxidative stress in ALS pathogenesis, and discuss several anti-inflammatory and anti-oxidative agents as potential therapeutics for ALS. Although oxidative stress and antioxidant fields are meaningful approaches to delay disease progression and prolong the survival in ALS, it is necessary to investigate various animal models or humans with different subtypes of sporadic and familial ALS. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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20. Elevated TRAF2/6 expression in Parkinson's disease is caused by the loss of Parkin E3 ligase activity.
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Chung, Ji-Yun, Park, Hee Ra, Lee, Su-Jin, Lee, Sun-Hye, Kim, Jin Sik, Jung, Youn-Sang, Hwang, Sang Hyun, Ha, Nam-Chul, Seol, Won-Gi, Lee, Jaewon, and Park, Bum-Joon
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- 2013
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21. Fermented Sipjeondaebo-tang Alleviates Memory Deficits and Loss of Hippocampal Neurogenesis in Scopolamine-induced Amnesia in Mice.
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Park, Hee Ra, Lee, Heeeun, Park, Hwayong, Cho, Won-Kyung, and Ma, Jin Yeul
- Published
- 2016
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