1. Timing and location dictate monocyte fate and their transition to tumor-associated macrophages.
- Author
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Dunsmore, Garett, Guo, Wei, Li, Ziyi, Bejarano, David Alejandro, Pai, Rhea, Yang, Katharine, Kwok, Immanuel, Tan, Leonard, Ng, Melissa, De La Calle Fabregat, Carlos, Yatim, Aline, Bougouin, Antoine, Mulder, Kevin, Thomas, Jake, Villar, Javiera, Bied, Mathilde, Kloeckner, Benoit, Dutertre, Charles-Antoine, Gessain, Grégoire, and Chakarov, Svetoslav
- Subjects
TRANSCRIPTION factors ,CELL populations ,PANCREATIC duct ,GENE expression profiling ,TUMOR growth - Abstract
Tumor-associated macrophages (TAMs) are a heterogeneous population of cells whose phenotypes and functions are shaped by factors that are incompletely understood. Herein, we asked when and where TAMs arise from blood monocytes and how they evolve during tumor development. We initiated pancreatic ductal adenocarcinoma (PDAC) in inducible monocyte fate-mapping mice and combined single-cell transcriptomics and high-dimensional flow cytometry to profile the monocyte-to-TAM transition. We revealed that monocytes differentiate first into a transient intermediate population of TAMs that generates two longer-lived lineages of terminally differentiated TAMs with distinct gene expression profiles, phenotypes, and intratumoral localization. Transcriptome datasets and tumor samples from patients with PDAC evidenced parallel TAM populations in humans and their prognostic associations. These insights will support the design of new therapeutic strategies targeting TAMs in PDAC. Editor's summary: Tumor-associated macrophages (TAMs) promote tumor growth and immune suppression, but how TAMs develop from circulating monocytes and local tissue-resident macrophages remains incompletely understood. Using a model of pancreatic ductal adenocarcinoma (PDAC) in monocyte fate-mapping mice, Dunsmore et al. examined the dynamics of the monocyte-to-macrophage transition in tumors. A population of "intermediate" TAMs gave rise to two more differentiated TAM subsets, distinguishable by their distinct surface marker phenotype, dependence on the transcription factor Maf, migratory behavior, and localization in tumors. These findings provide a time- and space-resolved picture of TAM heterogeneity and development in PDAC, which could guide the future development of TAM-targeted therapeutic strategies. —Claire Olingy [ABSTRACT FROM AUTHOR]
- Published
- 2024
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