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This article surveys recent studies about John the Baptist, focusing on disputed issues in contemporary research about this historical figure. After briefly reviewing the history of research as a framework for this survey, a presentation of the major contributions on John during the last decades follows. The article then reports on the status quaestionis of some still debated subjects related to the Baptist, such as the content of his message, portrayal as Elijah, relationship to Jesus, baptism, the account in Josephus, association with the Essenes/Qumran, and other minor matters. The work concludes by assessing what the author considers the most important research shifts and contributions of studies on John the Baptist in recent scholarship. [ABSTRACT FROM AUTHOR]

The attachment of porous media to a blunt trailing edge (TE) can significantly suppress vortex shedding processes and the related tonal noise, yet the near-wall and internal flow fields of porous media are difficult to analyze experimentally and rely on numerical simulations to elucidate the internal flow features. A structured porous trailing edge (SPTE) has been recently designed that follows a methodology of a structured porous coated cylinder. The SPTE acoustic response was compared against randomized porous media with 10 and 30 pores/in. in an anechoic wind tunnel over a range of flow velocities. Acoustic beamforming revealed that the dominant acoustic sources were at the end of the solid plate, even when a porous TE was attached. A region of integration was used to extract acoustic spectra without additional noise sources, revealing that the SPTE possesses superior noise reduction capability. Dipolar directivity patterns were observed at the vortex shedding frequency for each TE, and the coherence between microphones revealed the complex acoustic propagation of the high-frequency content. A wavelet analysis revealed how the SPTE breaks periodic vortex shedding cycles into smaller cycles over a wider frequency range, leading to an overall noise reduction relative to the other TEs. [ABSTRACT FROM AUTHOR]

Vertical-axis wind turbines (VAWTs) are considered promising solutions for urban wind energy generation due to their design, low maintenance costs, and reduced noise and visual impact compared to horizontal-axis wind turbines (HAWTs). However, deploying these turbines close to densely populated urban areas often triggers considerable local opposition to wind energy projects. Among the primary concerns raised by communities is the issue of noise emissions. Noise annoyance should be considered in the design and decision-making process to foster the social acceptance of VAWTs in urban environments. At the same time, maximising the operational efficiency of VAWTs in terms of power generation and actuation effort is equally important. This paper balances noise and aero-servo-elastic performance by formulating and solving a multi-objective optimisation problem from a controller calibration perspective. Psychoacoustic annoyance is taken as a novel indicator for the noise objective by providing a more reliable estimate of the human perception of wind turbine noise than conventional sound metrics. The computation of the psychoacoustic annoyance metric is made feasible by integrating it with an accurate and computationally efficient low-fidelity noise prediction model. For optimisation, an advanced partial-load control scheme – often used in industrial turbines – is considered, with the Kω2 controller as a baseline for comparison. Optimal solutions balancing the defined objectives are identified using a multi-criteria decision-making method (MCDM) and are subsequently assessed using a frequency-domain controller analysis framework and mid-fidelity time-domain aero-servo-elastic simulations. The MCDM results indicate the potential application of this controller in small-scale urban VAWTs to attain power gains of up to 39 % on one side and to trade off a reduction in actuation effort of up to 25 % at the cost of only a 2 % power decrease and a 6 % increase in psychoacoustic annoyance on the other side compared to the baseline. These findings confirm the flexible structure of the optimally calibrated wind speed estimator and tip-speed ratio (WSE–TSR) tracking controller, effectively balancing aero-servo-elastic performance with noise emissions and marking the first instance of integrating residential concerns into the decision-making process. [ABSTRACT FROM AUTHOR]

The genus Aspergillus harbors human infection-causing pathogens and is involved in the complex one-health challenge of antifungal resistance. Here, a 6-year retrospective study was conducted with Aspergillus spp. isolated from patients with invasive, chronic, and clinically suspected aspergillosis in a tertiary teaching hospital. A total of 64 Aspergillus spp. clinical isolates were investigated regarding molecular identification, biofilm, virulence in Galleria mellonella, antifungal susceptibility, and resistance to amphotericin B and azoles. Aspergillus section Fumigati (A. fumigatus sensu stricto, 62.5%) and section Flavi (A. flavus, 20.3%; A. parasiticus, 14%; and A. tamarii, 3.1%) have been identified. Aspergillus section Flavi clinical isolates were more virulent than section Fumigati clinical isolates. Furthermore, scant evidence supports a link between biofilm formation and virulence. The susceptibility of the Aspergillus spp. clinical isolates to itraconazole, posaconazole, voriconazole, and amphotericin B was evaluated. Most Aspergillus spp. clinical isolates (67.2%) had an AMB MIC value equal to or above 2 µg/mL, warning of a higher probability of therapeutic failure in the region under study. In general, the triazoles presented MIC values above the epidemiological cutoff value. The high triazole MIC values of A. fumigatus s.s. clinical isolates were investigated by sequencing the promoter region and cyp51A locus. The Cyp51A amino acid substitutions F46Y, M172V, N248T, N248K, D255E, and E427K were globally detected in 47.5% of A. fumigatus s.s. clinical isolates, and most of them are associated with high triazole MICs. Even so, the findings support voriconazole or itraconazole as the first therapeutic choice for treating Aspergillus infections. This study emphasizes the significance of continued surveillance of Aspergillus spp. infections to help overcome the gap in knowledge of the global fungal burden of infections and antifungal resistance, supporting public health initiatives. [ABSTRACT FROM AUTHOR]

A method for optimizing an automatic selection of values for parameters that feed segmentation algorithms is proposed. Evolutionary optimization techniques in combination with a fitness function based on a mutual information parameter have been used to find the optimal parameter values of region growing, fuzzy c‐means and graph cut segmentation algorithms. To validate the method, the segmentation of two transmission electron microscopy tomography reconstructed volumes of a carbon black‐reinforced rubber and a polylactic acid and clay nanocomposite is carried out (i) using evolutionary optimization techniques and (ii) manually by experts. The results confirm that the use of evolutionary optimization techniques, such as genetic algorithms, reduces the computational operation cost needed for a total grid search of segmentation parameters, reducing the probability of reaching a false optimum, and improving the segmentation quality. Highlights: A new approach to optimize 3D segmentation algorithms.Methodology to optimize segmentation parameters and improve segmentation quality.Improvement on the results when using region growing, fuzzy c‐means and graph cuts algorithms. [ABSTRACT FROM AUTHOR]

There are several research methods for the on-site assessment of U -values that aim to avoid the use of surface heat flux measurements and rely on tabulated or empirically developed correlations to define this parameter. This works performs a detailed process to estimate indoor surface heat transfer coefficients based on several experimental campaigns over building walls. Data is filtered out to remove periods with large temperature variations and/or unstable convective conditions due to HVAC. A statistical analysis is conducted, and the outcomes used to test the validity of U -value estimation approaches. The outcomes show that the actual surface heat transfer coefficients are in the range of reference works, but variations in the range of up to 2 W/m2 K are found. Uncertainty levels associated to the estimation of surface heat transfer coefficient are in the range 60% for instantaneous values while this is reduced down to 12%–20% for 8-h averages. Variations and uncertainty levels are higher for low temperature gradient situations, which are considered to be very likely for modern insulation levels. It is concluded that methods seeking to avoid the use of surface heat flux measurements need to develop much deeper knowledge in this field to gain accuracy and reliability. [ABSTRACT FROM AUTHOR]

The generation of a virtual, personal, auditory space to obtain a high-quality sound experience when using headphones is of great significance. Normally this experience is improved using personalized head-related transfer functions (HRTFs) that depend on a large degree of personal anthropometric information on pinnae. Most of the studies focus their personal auditory optimization analysis on the study of amplitude versus frequency on HRTFs, mainly in the search for significant elevation cues of frequency maps. Therefore, knowing the HRTFs of each individual is of considerable help to improve sound quality. The following work proposes a methodology to model HRTFs according to the individual structure of pinnae using multilayer perceptron and linear regression techniques. It is proposed to generate several models that allow knowing HRTFs amplitude for each frequency based on the personal anthropometric data on pinnae, the azimuth angle, and the elevation of the sound source, thus predicting frequency magnitudes. Experiments show that the prediction of new personal HRTF generates low errors, thus this model can be applied to new heads with different pinnae characteristics with high confidence. Improving the results obtained with the standard KEMAR pinna, usually used in cases where there is a lack of information. [ABSTRACT FROM AUTHOR]

The dorsolateral striatum (DLS) receives excitatory inputs from both sensory and motor cortical regions. In the neocortex, sensory responses are affected by motor activity, however, it is not known whether such sensorimotor interactions occur in the striatum and how they are shaped by dopamine. To determine the impact of motor activity on striatal sensory processing, we performed in vivo whole-cell recordings in the DLS of awake mice during the presentation of tactile stimuli. Striatal medium spiny neurons (MSNs) were activated by both whisker stimulation and spontaneous whisking, however, their responses to whisker deflection during ongoing whisking were attenuated. Dopamine depletion reduced the representation of whisking in direct-pathway MSNs, but not in those of the indirect-pathway. Furthermore, dopamine depletion impaired the discrimination between ipsilateral and contralateral sensory stimulation in both direct and indirect pathway MSNs. Our results show that whisking affects sensory responses in DLS and that striatal representation of both processes is dopamine- and cell type-dependent.The basal ganglia process sensory and motor related information, but it is not known how movement affects sensory integration. Here, the authors show using in vivo whole-cell recordings that striatal neurons respond to both sensory stimuli and spontaneous whisking and that sensory responses are attenuated by whisking. [ABSTRACT FROM AUTHOR]

Automatic guidance vehicles (AGV) are industrial vehicles that play an important role in the development of smart manufacturing systems and Industry 4.0. To provide these autonomous systems with the flexibility that is required today in these industrial workspaces, AGV computational models are necessary in order to analyze their performance and design efficient planning and control strategies. To address these issues, in this work, the mathematical model and the algorithm that implement a computational control-oriented simulation model of a hybrid tricycle-differential AGV with multi-trailers have been developed. Physical factors, such as wheel-ground interaction and the effect of vertical and lateral loads on its dynamics, have been incorporated into the model. The model has been tested in simulation with two different controllers and three trajectories: a circumference, a square, and an s-shaped curve. Furthermore, it has been used to analyze extreme situations of slipping and capsizing and the influence of the number of trailers on the tracking error and the control effort. This way, the minimum lateral friction coefficient to avoid slipping and the minimum ratio between the lateral and height displacement of the center of gravity to avoid capsizing have been obtained. In addition, the effect of a change in the friction coefficient has also been simulated. [ABSTRACT FROM AUTHOR]

Wind flow on a bluff body is a complex and nonlinear phenomenon that has been mainly studied experimentally or analytically. Several mathematical methods have been developed to predict the wind-induced pressure distribution on bluff bodies; however, most of them result unpractical due to the mathematical complexity required. Long-short term memory artificial neural networks with deep learning have proven to be efficient tools in the solution of nonlinear phenomena, although the choice of a more efficient network model remains a topic of open discussion for researchers. The main objective of this study is to develop long-short term memory artificial neural network models to predict the external pressure distribution of a low-rise building. For the development of the artificial neural network models, the multilayer perceptron and the recurrent neural network were also employed for comparison purposes. To train the artificial neural networks, a database with the external pressure coefficients from boundary layer wind tunnel tests of a low-rise building is employed. The analysis results indicate that the long-short term memory artificial neural network model and the multilayer perceptron neural network outperform the recurrent neural network. [ABSTRACT FROM AUTHOR]

Alpha-synuclein (αSyn) is a protein involved in neurodegenerative disorders including Parkinson's disease. Amyloid formation of αSyn can be modulated by the 'P1 region' (residues 36-42). Here, mutational studies of P1 reveal that Y39A and S42A extend the lag-phase of αSyn amyloid formation in vitro and rescue amyloid-associated cytotoxicity in C. elegans. Additionally, L38I αSyn forms amyloid fibrils more rapidly than WT, L38A has no effect, but L38M does not form amyloid fibrils in vitro and protects from proteotoxicity. Swapping the sequence of the two residues that differ in the P1 region of the paralogue γSyn to those of αSyn did not enhance fibril formation for γSyn. Peptide binding experiments using NMR showed that P1 synergises with residues in the NAC and C-terminal regions to initiate aggregation. The remarkable specificity of the interactions that control αSyn amyloid formation, identifies this region as a potential target for therapeutics, despite their weak and transient nature. The authors of this work characterize the effect of amino acid substitution on α-synuclein (α-Syn) aggregation. Residues 38 and 42 (in addition to 39) within the P1 region of α-Syn affect amyloid formation. The effect of substitution at position 38 is dependent on the amino-acid introduced, suggesting that specific interactions control α -Syn aggregation. [ABSTRACT FROM AUTHOR]

This work presents the quality performance and the capabilities of altimetry derived wind speed (WS) retrievals from the altimeters on-board Copernicus satellites Sentinel-3A/B (S3A/B) for the spatial assessment of WS outputs from the weather research and forecasting (WRF) model over the complex area of the Gulf of Cádiz (GoC), Spain. In order to assess the applicability of the altimetry data for this purpose, comparisons between three different WS data sources over the area were evaluated: in situ measurements, S3A/B 20 Hz altimetry data, and WRF model outputs. Sentinel-3A/B WS data were compared against two different moored buoys to guarantee the quality of the data over the GoC, resulting in satisfying scores (average results: RMSE = 1.21 m/s, r = 0.93 for S3A and RMSE = 1.36 m/s, r = 0.89 for S3B). Second, the WRF model was validated with in situ data from four different stations to ensure the correct performance over the area. Finally, the spatial variability of the WS derived from the WRF model was compared with the along-track altimetry-derived WS. The analysis was carried out under different wind synoptic conditions. Qualitative and quantitative results (average RMSE < 1.0 m/s) show agreement between both data sets under low/high wind regimes, proving that the spatial coverage of satellite altimetry enables the spatial assessment of high-resolution numerical weather prediction models in complex water-covered zones. [ABSTRACT FROM AUTHOR]

Photobiomodulation has been used to inactivate bacterial growth, in different laser or LED protocols. Thus, the aim of this study was to verify the inhibition of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, in ATCC strains and bacteria collected from patients with skin burns, after irradiation with LED; 300 μl of saline solution with bacterial suspension was irradiated at a concentration of 0.5–0.63, by the McFarland scale, after five serial dilutions, with evaluation of pre- and post-irradiation pH and temperature control. The cultures were placed in a bacteriological incubator at 37 °C for 24 h for later counting of colony-forming units (CFU). Data were analyzed by Shapiro–Wilk tests and single-factor ANOVA, with Tukey post hoc (p < 0.05). Both wavelengths and energy densities tested showed inhibition of bacterial growth. The comparison of the irradiated groups (ATCC) with the control group showed the following: S. aureus and P. aeruginosa 465 nm (40 J/cm2) and 630 nm (50 J/cm2) and E. coli 465 nm (40 J/cm2) and 630 nm (30 J/cm2). Among the ATCC S. aureus groups, there was a difference for 630 nm (30 J/cm2) and 465 nm (30, 40, 50 J/cm2). The bacteria from the burned patients were S. aureus (30 and 50 J/cm2) and P. aeruginosa (50 J/cm2). We conclude that different bacterial strains were reduced into colony-forming units after LED irradiation. [ABSTRACT FROM AUTHOR]

Human islet amyloid polypeptide (hIAPP) self-assembles into amyloid fibrils which deposit in pancreatic islets of type 2 diabetes (T2D) patients. Here, we applied chemical kinetics to study the mechanism of amyloid assembly of wild-type hIAPP and its more amyloidogenic natural variant S20G. We show that the aggregation of both peptides involves primary nucleation, secondary nucleation and elongation. We also report the discovery of two structurally distinct small-molecule modulators of hIAPP assembly, one delaying the aggregation of wt hIAPP, but not S20G; while the other enhances the rate of aggregation of both variants at substoichiometric concentrations. Investigation into the inhibition mechanism(s) using chemical kinetics, native mass spectrometry, fluorescence titration, SPR and NMR revealed that the inhibitor retards primary nucleation, secondary nucleation and elongation, by binding peptide monomers. By contrast, the accelerator predominantly interacts with species formed in the lag phase. These compounds represent useful chemical tools to study hIAPP aggregation and may serve as promising starting-points for the development of therapeutics for T2D. Here the authors carry out chemical kinetic studies revealing that aggregation of hIAPP and its variant S20G involves secondary nucleation. Two small molecules with novel scaffolds are shown to inhibit or accelerate aggregation by binding different molecular species. [ABSTRACT FROM AUTHOR]

Introduction: The recalcitrant nature of patients with acute exacerbation of chronic rhinosinusitis (AECRS) potentially involves persisting colonization of the sinonasal mucosa by bacterial biofilms. Biofilms are known to be highly resistant to antibiotics, which may trigger or maintain chronic inflammation in the sinonasal mucosa. However, little is known about the relationship between the minimum inhibitory concentration (MIC) and antibiofilm concentrations of bacteria obtained from AECRS patients. Material and Methods: Thirty bacterial strains from 25 patients with AECRS were identified and underwent MIC determination (VITEK® 2). The planktonic isolates were submitted to an in vitro formation of biofilms (Modified Calgary Biofilm Device) and determination of minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) for amoxicillin, amoxicillin/clavulanic acid, clarithromycin, and levofloxacin. MIC of the planktonic forms was compared with MBIC and MBEC levels, according to the breakpoints established by the Clinical Laboratory Standards Institute guidelines. Results: The main bacteria retrieved was S. aureus (60%), followed by other Gram-positive and Gram-negative bacteria in lower frequencies. 76.7% of strains formed biofilm in vitro (n=23/30). The planktonic isolates presented high rates of resistance for amoxicillin (82.6%) and clarithromycin (39.1%), and lower rates for amoxicillin/clavulanic acid (17.4%). The biofilm-forming bacteria counterparts presented higher levels of MBIC and MBEC compared to the MIC levels for amoxicillin, amoxicillin/clavulanic acid, and clarithromycin. Levofloxacin was highly effective against both planktonic and biofilm forms. Planktonic resistant forms were associated with levels of antibiofilm concentrations (MBIC and MBEC). Conclusions: Biofilm-forming bacteria from AECRS patients are prevalent, and biofilm forms are highly resistant to antibiotics compared to their planktonic counterparts. Antibiotic resistance observed in planktonic forms is a good indicator of biofilm resistance, although near 20% of susceptible planktonic bacteria can produce antibiotic tolerant biofilms. [ABSTRACT FROM AUTHOR]

The present investigation refers to the improvement of tensile properties, hardness, and creep resistance obtained from a high-chromium novel steel grade formulated by the combination of innovative steel composition and heat treatment. The novel steel grade has evolved from the P92 steel grade, and shows a better behaviour than that offered by the P92 steel grade, thus representing a promising metallurgical strategy for increasing the efficiency of power plants, and subsequently reducing the CO2 emissions. [ABSTRACT FROM AUTHOR]

In the tyrosine kinase inhibitor era, the blast phase of chronic myeloid leukaemia (BP-CML) renders an uncommon presentation and has a poor prognosis with an estimated overall survival below 20%. Mixedphenotype blast phase is even more infrequent, presenting in 3.3% of these patients. Blast phase manifests along haematological sarcomas, with extramedullary activity in lymph nodes, skin and bone. We report the case of a patient with an ovarian sarcoma as an extramedullary presentation of mixed-phenotype BP-CML refractory to conventional treatment which responded to immunotherapy against CD33 and CD19. [ABSTRACT FROM AUTHOR]

Background: Tuberculosis is an infectious disease caused by the bacillus Mycobacterium tuberculosis. Compounds including a sulfur-containing scaffold have been shown to be key scaffolds in various antituberculosis agents. Interestingly, the 3-hydroxy-3-phenyl-prop-2-enedithioic acids 11a-j have, to the best of our knowledge, not been previously described as antituberculosis agents. Purpose: In the present study, we investigated the role of substituents attached to the phenyl ring of a 3-hydroxy-3-phenyl-prop-2-enedithioic acid scaffold (compounds 11a–j) in inhibiting the growth of M. tuberculosis strain H37Rv. Methods: (Z)-3-hydroxy-3-(4-R-phenyl)-prop-2-enedithioic acids 11b–j , with R groups including various electron-donating or electron-withdrawing groups, were designed by structurally modifying the lead compound 11a. The syntheses of 11a–j involved each one-step procedure starting from the corresponding substituted acetophenone. Compounds 11a–j were tested against M. tuberculosis strain H37Rv to evaluate their bacterial growth inhibitory activities. ADMET profiles were predicted by employing three different methods. In addition, molecular docking studies were carried out, based on the molecular similarities of the synthesized compounds with ethionamide (5), on the active site of the M. tuberculosis H37Rv (3R)-hydroxyacyl-ACP (HadAB) dehydratase heterodimer. Results: The antituberculosis activities of compounds 11a–j could be explained in terms of the presence of electron-donating or electron-withdrawing substituents on the aromatic ring of the substituted 3-hydroxy-3-phenyl)-prop-2-enedithioic acid core. The activity and selectivity index (SI) value of (Z)-3-hydroxy-3-(4-nitrophenyl)-prop-2-enedithioic acid 11e suggested that this compound could be used for the design of novel antituberculosis agents. Most of the synthesized molecules showed an acceptable ADME profile and a low probability of being toxic. Docking studies of 11d and 11e showed them forming hydrogen bonds with the ACys61 residue of the HadAB enzyme. Conclusion: Our results suggested that the antituberculosis compound 11e could be used for the design of novel antituberculosis agents. [ABSTRACT FROM AUTHOR]

Although the erythema migrans (EM) skin rash is traditionally considered a hallmark of Lyme disease, other dermatological manifestations of the tickborne disease are less well known. We describe a 49-year-old woman with erosive genital ulcerations, secondary EM rashes and jagged skin lesions associated with Lyme disease. The skin rashes exhibited fibers characteristic of Morgellons disease. Molecular testing confirmed the presence of Borrelia DNA in both vaginal culture and serum specimens. In further studies on a secondary EM lesion containing filaments, Gömöri trichrome staining revealed the presence of collagen in the filaments, while Dieterle and anti-Borrelia immunostaining revealed intracellular and extracellular Borrelia organisms. Intracellular staining for Borrelia was also observed in lymphocytic infiltrates. Lyme disease may present with a variety of genital lesions and dermatological manifestations including Morgellons disease. Careful evaluation is required to determine the presence of Borrelia organisms associated with these dermopathies. [ABSTRACT FROM AUTHOR]

This paper describes the evaluation of a single output, online, and time domain modal parameters identification technique based on differential algebra and operational calculus. In addition, an analysis of the frequency response function (FRF) of the system is conducted in a specific set up, emulating its nominal or operational conditions to determine the influence of the non-linearities over the dynamic behavior of the system in those particular magnitudes of deformations; thus, this influence is quantified by a numerical index. This methodology is applied to a wind turbine blade submitted to wind tunnel experiments. The natural frequencies and modal damping ratios of six bending modes associated with the blade are estimated using real-time velocity measurements from one single point of the blade. A comparison with the usual impact hammer modal testing is performed to evaluate and establish the proposed approach's main contributions. The developed modal parameter identification algorithms are implemented to run into a standard personal computer (PC) where the data acquisition system's measurements are conditioned and processed. The results show the performance and the fast parametric estimation of the proposed algebraic identification approach. [ABSTRACT FROM AUTHOR]

Copyright of Iberoamerican Journal of Industrial Engineering is the property of Iberoamerican Journal of Industrial Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Summary: Background: Cryptococcus neoformans/ Cryptococcus gattii species complex is composed of encapsulated yeast species that are causative agents of cryptococcosis. The characterisation of pathogenic Cryptococcus species provides useful data for epidemiological studies as well as the clinical diagnosis and treatment of patients. Objectives: This study aimed to characterise the epidemiology, antifungal susceptibility and virulence of 72 clinical strains isolated from cryptococcosis cases between 2012 and 2017 in a tertiary reference hospital in south‐eastern Brazil. Methods: Species and molecular types were molecularly assessed by PCR and PCR‐restriction fragment length polymorphism (RFLP) of the URA5 gene. Antifungal susceptibility testing was performed according to the CLSI protocols. The virulence was studied in a Galleria mellonella infection model. Results: The most frequently isolated strain was C. neoformans molecular type VNI (61/72; 84.7%), although C. neoformans molecular type VNII (3/72; 4.2%) was also isolated. Additionally, C. deuterogattii molecular type VGII (8/72; 11.1%) was present, but most frequently from non‐HIV‐infected patients. Non‐wild‐type phenotype to the antifungals was observed in 26.4% (19/72) of the C. neoformans and C. deuterogattii clinical isolates, and the latter demonstrated higher MIC to fluconazole and itraconazole than C. neoformans clinical isolates. Finally, the virulence of C. neoformans and C. deuterogattii clinical isolates was diverse in G mellonella larvae and uncorrelated with the virulence factors of melanin and capsule. Conclusions: The assessment of the spread of cryptococcal species and molecular types as well as the pattern of corresponding antifungal susceptibility and virulence aids in surveil the emergence of resistant strains, ensuring more accurate management of the cryptococcal infection. [ABSTRACT FROM AUTHOR]

Dear Editor Paraccoccidioidomycosis (PCM) is an endemic disease caused by the fungus I Paracoccidioidomycosis brasilensis i ,1,2 which when inhaled intensely and/or continuously reaches terminal bronchioles and alveoli promoting, in most of the cases, primary pulmonary paracoccidioidomycosis (PPCM).2,3 While effective treatment is available to control PPCM, many patients considered clinically cured have developed pulmonary hypertension (PH). Vascular adventitial layer collagen fibers deposition was significantly increased in PCM patients compared to the chronic obstructive pulmonary disease (COPD)/emphysema group without PH ( I P i < .05)(Figure 2), although COPD/emphysema is a known cause of vascular remodeling. GLO:F2H7/01nov20:ctm2213-fig-0002.jpg PHOTO (COLOR): 2 Human pulmonary paracoccidioidomycosis induced pulmonary hypertension. lung biopsy from PPCM patients and timeframe for development of pulmonary hypertension. [Extracted from the article]

Copyright of Revista Verde de Agroecologia e Desenvolvimento Sustentável is the property of Revista Verde de Agroecologia e Desenvolvimento Sustentavel and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The cosmetic use of devices like prostheses to increase breast volume is nothing new. It is calculated that millions of people have been exposed to silicone in several ways, including breast implants, and since 1964 there has been uncertainty regarding their safety. We did not find in the literature any studies that reported the appearance of a specific immunological disease in patients with silicone breast implants. Furthermore, there are also neither case–control studies nor reports of patients proving that symptoms of autoimmune/inflammatory syndrome induced by adjuvants (ASIA) occurred after the placement of silicone implants nor that the patients had pre-existing symptoms. Several studies link silicone to allergic reactions and the development of systemic autoimmune diseases; however, other studies deny this association. There are currently several theories about the effect of silicone on the body. One theory with greater acceptance proposes an adjuvant effect of silicone on the development of autoimmune diseases in genetically predisposed patients. However, the variety of symptoms occurring in patients who develop these pathologies leads to doubts about the relationship between the adjuvant effects of a silicone prosthesis may have with a specific autoimmune disease or a mix of these diseases. The lack of consensus on this topic obliges a full review of what has already been reported in the literature to integrate the knowledge and propose a focus for new research on this matter. Level of Evidence IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. [ABSTRACT FROM AUTHOR]

Paracoccidioides brasiliensis complex and P. lutzii are the etiological agents of paracoccidioidomycosis. The geographic distribution of these species in South America is still poorly comprehended. Fifty samples of Paracoccidioides spp. were genotyped, with 46 clinical isolates predominantly isolated in the geographic area of Ribeirão Preto, SP, and four environmental isolates collected in Ibiá, MG, southeastern Brazil. These isolates were evaluated by PCR-RFLP (Restriction Fragment Length Polymorphism) of the tub1 gene and the sequencing of the gp43 exon 2 loci. The species P. lutzii was confirmed by sequencing the internal transcribed spacer (ITS) region of the ribosomal DNA. P. brasiliensis sensu stricto S1b (n = 42) and S1a (n = 5), P. americana (n = 1), P. restrepiensis (n = 1), and P. lutzii (n = 1) were identified among the clinical isolates. All the environmental isolates were characterized as P. brasiliensis sensu stricto S1b. The patient infection by P. lutzii, P. americana (PS2), and one isolate of P. brasiliensis sensu stricto S1b most likely occurred in a geographic area far from the fungal isolation site. No association was found between the infecting genotype and the disease form. These results expand the knowledge of the Paracoccidioides species distribution and emphasize that human migration must also be considered to pinpoint the genotypes in the endemic area. [ABSTRACT FROM AUTHOR]

The Tepary bean (Phaseolus acutifolius) lectin fraction (TBLF) exhibits differential cytotoxicity on colon cancer cells and inhibition of early tumorigenesis in the colon (50 mg/kg, three times per week, for 6 weeks). TBLF showed low toxicity with the ability to activate the immune system; however, some adverse effects are the loss in body weight gain, intestinal atrophy, and pancreatic hyperplasia. After a recovery period of 2 weeks after treatment, reversion of pancreatic hyperplasia but no recovery of intestinal atrophy was observed. As TBLF has shown anticancer effects on the colon, it is important to characterize the adverse effects and how they can be reversed. Sprague Dawley rats were administered with TBLF (50 mg/kg) for 6 weeks, three times per week, and then allowed to recover for 6 weeks post-treatment. After TBLF administration, small intestine atrophy, villus atrophy, and cryptic hyperplasia were confirmed, as well as increased intestinal mucus production, increased permeability and a decrease in the apparent ileal digestibility of crude proteins. The colon showed damage in the simple prismatic tissue and decreased crypt depth, and changes in microbiota and a decrease in the apparent fecal digestibility of crude protein were determined. Our results show that the adverse effects provoked by TBLF were partially reversed after 6 weeks of recovery post-treatment, suggesting that increasing the recovery period it could be possible to reverse all adverse effects observed. [ABSTRACT FROM AUTHOR]

Background: A significant difference exists between the published results reporting the clinical outcome following brain arteriovenous malformation (AVM) ruptures. Information about the outcome following hemorrhage in an AVM population treated with radiosurgery could provide additional information to assess the risk of mortality and morbidity following an AVM hemorrhage. Methods: Clinical outcome was studied in 383 patients, the largest patient population yet studied, who suffered from a symptomatic hemorrhage after Gamma Knife® surgery (GKS) but before confirmed AVM obliteration. The impact of different patient, AVM, and treatment parameters on the clinical outcome was analyzed. The aim was to generate outcome predictions by comparing our data to and combining them with earlier published results. Results: No relation was found between clinical outcome and treatment parameters, indicating that the results are applicable also on untreated AVMs. Twenty-one percent of the patients died, 45% developed or experienced worsening of neurological sequelae, and 35% recovered completely after the hemorrhage. Old age was a predictor of poor outcome. Sex, AVM location, AVM volume, and history of prior hemorrhage did not influence the outcome. The mortality rate was comparable to earlier published prospective data, but higher than that found in retrospective studies. Conclusions: The mortality rates in earlier published retrospective series as well as in studies focusing on clinical outcome following AVM hemorrhage significantly underestimate the risk for a mortal outcome following an AVM hemorrhage. Based on our findings, an AVM rupture has around 20% likelihood to result in mortality, 45% likelihood to result in a minor or major deficit, and 35% likelihood of complete recovery. The findings are probably applicable also for AVM ruptures in general. The cumulative mortality and morbidity rates 25 years after diagnosis were estimated to be around 40% in a patient with a patent AVM. [ABSTRACT FROM AUTHOR]

Photoresponsive molecules are present in many organisms, including animals and plants. These kinds of pigments absorb UV or visible radiation and efficiently dissipate the photon energy through pathways that involve intramolecular rearrangements. In this work, a set of bisindole analogs of the natural pigment caulerpin were synthesized and evaluated as photoresponsive systems. The bisindoles of this study are modified versions of the caulerpin metabolite which conserve an asymmetric methinic bridge between two indolyl moieties. In the ground state the more stable conformation for these systems was found to be the E-isomer. Detailed NMR experiments show that upon UVA irradiation (400 nm) the bisindoles evolve to the highly stable Z-isomer, which is persistent in solution for several weeks in the dark. Femtosecond resolved measurements revealed a 300 fs deactivation channel for the first singlet excited state which is associated with evolution in the S1 states leading to ultrafast E–Z photoisomerization and a return to the electronic ground state. The excited-state dynamics were also characterized by mapping changes in the electron density in the first singlet excited state. This study shows evident charge density migration away from the double-bond region upon irradiation. The redistribution of the electron density is followed by pyramidalization of the adjacent carbon atoms while torsion takes place. The synthesized bisindolic core of this study can lead to the development of novel and easy to functionalize photoresistant and photoresponsive molecules, potentially applicable as photo-controllable molecular switches. [ABSTRACT FROM AUTHOR]

Selenium (Se) deficiency in the human population is one of the major nutritional problems in the world. A possible solution is biofortification. Biofortification is a process that increases the element of interest's concentration in food by fertilizer applications (agronomic biofortification) or by genetic breeding focused on accumulation capacity for this element (genetic biofortification). Thus, the aim of this work was to determine the effect of Se doses on biofortification efficiency and agronomic traits of cowpea cultivars. The factors here studied were five doses of Se (0, 10, 20, 30 and 40 g ha−1), using sodium selenate (Na2SeO4) as Se source, and three cultivars of cowpea (BRS-Cauamé, BRS-Guariba, and BRS-Itaim). Foliar application of selenate allows biofortification of selenium in cowpea grains without an impact on growth, development, and grain yield. The biofortification of Se in cowpea seeds can contribute to the food security of the low-income population of tropical regions. [ABSTRACT FROM AUTHOR]

Introduction: Morgellons disease (MD) is a contested dermopathy that is associated with Borrelia spirochetal infection. A simple classification system was previously established to help validate the disease based on clinical features (classes I-IV). Methods: Drawing on historical and pathological parallels with syphilis, we formulated a more detailed staging system based on clinical features as well as severity of skin lesions and corresponding histopathological infection patterns, as determined by anti-Borrelia immunohistochemical staining. Results: Clinical classes I-IV of MD are further categorized as mild, moderate and severe, or stages A, B and C, respectively, based on histopathological findings. Stage A lesions demonstrated little or no immune infiltrates and little or no disorganization of cells; macrophages were not present, and hemorrhage was negligible. Extracellular isolated spirochetes and intracellular staining of keratinocytes in the lower epidermis was occasionally seen. Stage C lesions demonstrated positive staining of keratinocytes in the stratum basale and stratum spinosum and positive intracellular staining of macrophages for Borrelia. Aggregate Borrelia colonies were frequently encountered, hemorrhage was frequent, and intracellularly stained fibroblasts were occasionally seen. Stage B lesions demonstrated a pattern intermediate between Stages A and C. Conclusion: The enhanced staging system provides objective criteria to assess the severity of dermopathy in MD. Further studies are needed to determine the optimal treatment for MD based on this staging system related to Borrelia infection. [ABSTRACT FROM AUTHOR]

Paracoccidioidomycosis caused by Paracoccidioides lutzii is endemic in the Midwest of Brazil and its clinical spectrum is still little known due to the recent identification of this fungal species. A patient resident in Southeast Brazil, but who had lived for many years in the Midwest region, presented with skin injuries, chronic cough and bilateral adrenal involvement. Paracoccidioides spp. was isolated in culture from a skin lesion biopsy. This isolate was later identified as P. lutzii using gene sequencing. A favorable initial response to treatment with itraconazole was observed, but a few weeks later, the patient developed respiratory failure and worsening of lung lesions. Evaluation by computed tomography and echocardiography were suggestive of pulmonary arterial hypertension, and a bronchoscopic biopsy showed peribronchial remodeling. The patient completed the antifungal treatment but maintained the respiratory dysfunction. The reported case shows that P. lutzii can be isolated from patients in a geographic area far from the place of infection acquisition and that, as P. brasiliensis, it can cause adrenal injury and cardio-respiratory complications as a consequence of excessive necrosis and fibrosis. [ABSTRACT FROM AUTHOR]

Within the pests that affect the avocado crop we find Oligonychus perseae or better known as crystalline spider. This mite produces affectations in the epidermis of the leaves discoloring the injured areas, in severe attacks they cause defoliation and low production, all this as a result of the extraction of the cellular content of the tissues. In the State of Mexico, there are about 10 000 hectares planted with avocado, with an average production of 15. 602 t ha-1. The spatial distribution of O. perseae in four municipalities producing avocado var. Hass through the application of geostatistical analysis and ordinary krigeado. The results show that the crystalline spider populations are presented in an aggregate way, adjusting mostly spherical models. The area infested with the mite exceeded 73% of the municipality in all samples and the maximum number of individuals found was 1 445 organisms per tree. The most affected municipalities were Coatepec Harinas and Donato Guerra. [ABSTRACT FROM AUTHOR]

In carbon black reinforced rubbers, the shape of the carbon black aggregates has a very significant influence on the final properties of the material. Accurately classifying these particles by shape has proven to be difficult, but the results of the classification would allow to model the final mechanical properties of the material. In this work, 21 features are measured from 7714 isolated filler images obtained from TEM images and used for the classification. Support vector machines and artificial neural network techniques are used to classify the aggregates using a methodology to tune the algorithm parameters to improve the performance of the models. Also, genetic algorithms are applied to make a feature selection in order to get most robust and accurate models. It is demonstrated that the combination of genetic algorithms with support vector machines and artificial neural network improves the classification results and minimizes the complexity of the resulting model. [ABSTRACT FROM AUTHOR]

Engine noise shielding is an important measure towards low-noise aircraft configurations. Such designs are supported by prediction tools that indicate high values for shielding of engine noise. Most prediction models approximate the complex nature of engine noise to simple noise sources such as monopoles or dipoles. This work compares predictions of noise shielding with experiments using different noise sources and shielding body geometries. The experiments considered in this work concern a monopole source shielded by a flat plate and a NACA 64-008 A wing, and a propeller shielded by the same wing. Comparisons between models and measurements are made by analysis of noise levels at individual microphones and using conventional beamforming. Results show that for the monopole cases the model predictions are in agreement with the experimental data, with an average deviation of 2–3 dB. The curvature of the leading edge of the wing influences the noise shielding results. The measured values of noise shielding of propeller noise are lower than those measured for the omni-directional source. Different types of source directivity are used to approximate the propeller in the predictions: monopole, dipole and a multi-source. The dipole approximation shows the best agreement with the experiments for the case of the propeller. [ABSTRACT FROM AUTHOR]