1. MSIpixel: a fully automated pipeline for compound annotation and quantitation in mass spectrometry imaging experiments.
- Author
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Morosi, Lavinia, Miotto, Matteo, Timo, Sara, Carloni, Sara, Bruno, Eleonora, Meroni, Marina, Menna, Elisabetta, Lodato, Simona, Rescigno, Maria, and Martano, Giuseppe
- Subjects
ANNOTATIONS ,COMPLEX matrices ,SINGLE molecules ,SMALL molecules ,METABOLOMICS - Abstract
Mass spectrometry imaging (MSI) is commonly used to map the spatial distribution of small molecules within complex biological matrices. One of the major challenges in imaging MS-based spatial metabolomics is molecular identification and metabolite annotation, to address this limitation, annotation is often complemented with parallel bulk LC-MS2-based metabolomics to confirm and validate identifications. Here we applied MSI method, utilizing data-dependent acquisition, to visualize and identify unknown molecules in a single instrument run. To reach this aim we developed MSIpixel, a fully automated pipeline for compound annotation and quantitation in MSI experiments. It overcomes challenges in molecular identification, and improving reliability and comprehensiveness in MSI-based spatial metabolomics. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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