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This paper presents the design of a multimodal chatbot for use in an interactive theater performance. This chatbot has an architecture consisting of vision and natural language processing capabilities, as well as embodiment in a non-anthropomorphic movable LED array set in a stage. Designed for interaction with up to five users at a time, the system can perform tasks including face detection and emotion classification, tracking of crowd movement through mobile phones, and real-time conversation to guide users through a nonlinear story and interactive games. The final prototype, named ODO, is a tangible embodiment of a distributed multimedia system that solves several technical challenges to provide users with a unique experience through novel interaction. [ABSTRACT FROM AUTHOR]

Small RNAs (21-24 nt) are involved in gene regulation through translation inhibition, mRNA cleavage, or directing chromatin modifications. In rice, currently ≈240 microRNAs (miRNAs) have been annotated. We sequenced more than four million small RNAs from rice and identified another 24 miRNA genes. Among these, we `found a unique class of miRNAs that derive from natural cis-antisense transcript pairs. This configuration generates miRNAs that can perfectly match their targets. We provide evidence that `the miRNAs function by inducing mRNA cleavage in the middle of their complementary site. Their production requires Dicer-like 1 (DCL1) activity, which is essential for canonical miRNA biogenesis., All of the natural antisense miRNAs (nat-miRNAs) identified in this study have large introns in their precursors that appear critical for nat-miRNA evolution and for the formation of functional mIRNA loci. These findings suggest that other natural cis-antisense loci with similar exon-intron arrangements could be another source of miRNA genes. [ABSTRACT FROM AUTHOR]

Identification of all expressed transcripts in a sequenced genome is essential both for genome analysis and for realization of the goals of systems biology. We used the transcriptional profiling technology called 'massively parallel signature sequencing' to develop a comprehensive expression atlas of rice (Oryza sativa cv Nipponbare). We sequenced 46,971,553 mRNA transcripts from 22 libraries, and 2,953,855 small RNAs from 3 libraries. The data demonstrate widespread transcription throughout the genome, including sense expression of at least 25,500 annotated genes and antisense expression of nearly 9,000 annotated genes. An additional set of ∼15,000 mRNA signatures mapped to unannotated genomic regions. The majority of the small RNA data represented lower abundance short interfering RNAs that match repetitive sequences, intergenic regions and genes. Among these, numerous clusters of highly regulated small RNAs were readily observed. We developed a genome browser (http://mpss.udel.edu/rice) for public access to the transcriptional profiling data for this important crop. [ABSTRACT FROM AUTHOR]

The article presents a case study of a 29-year old and a 32-year old man who suffer asymmetrical cerebellar syndrome with pyramidal and bulbar dysfunction of subacute.