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The role of Artificial intelligence in achieving high performance in manufacturing systems has been explored over the years. However, with the increasing number of variants in the factories and the advances in digital technologies new opportunities arise for supporting operators in the factory. The hybrid production systems stipulate the efficient collaboration of the workers with the machines. Human action recognition is a major enabler for intuitive machines and robots to achieve more efficient interaction with workers. This paper discusses a software framework called Praxis, aiming to facilitate the deployment of human action recognition (HAR) in assembly. Praxis is designed to provide a flexible and scalable architecture for implementing human action recognition in assembly lines. The framework has been implemented in a real-world case study originating for showcasing and validating the effectiveness of Praxis in real-life applications. It is deployed in an assembly use case for an air compression production industry. This study highlights the potential of the Praxis framework for promoting efficient human–robot collaboration (HRC) in modern manufacturing environments through HAR. [ABSTRACT FROM AUTHOR]

Allergen-specific immunotherapy (AIT) induces immune tolerance, showing the highest success rate (>95%) for insect venom while a much lower chance for pollen allergy. However, the molecular switches leading to successful durable tolerance restoration remain elusive. The primary outcome of this observational study is the comprehensive immunological cellular characterization during the AIT initiation phase, whereas the secondary outcomes are the serological and Th2-cell-type-specific transcriptomic analyses. Here we apply a multilayer-omics approach to reveal dynamic peripheral immune landscapes during the AIT-initiation phase in venom allergy patients (VAP) versus pollen-allergic and healthy controls. Already at baseline, VAP exhibit altered abundances of several cell types, including classical monocytes (cMono), CD4+ hybrid type 1-type 17 cells (Th1-Th17 or Th1/17) and CD8+ counterparts (Tc1-Tc17 or Tc1/17). At 8-24 h following AIT launch in VAP, we identify a uniform AIT-elicited pulse of late-transitional/IL-10-producing B cells, IL-6 signaling within Th2 cells and non-inflammatory serum-IL-6 levels. Sequential induction of activation and survival protein markers also immediately occur. A disequilibrium between serum IL-6 and cMono in VAP baseline is restored at day seven following AIT launch. Our longitudinal analysis discovers molecular switches during initiation-phase insect-venom AIT that secure long-term outcomes. Trial number: NCT02931955. Allergen specific immunotherapy (AIT) is both, safe and effective in reducing systemic symptoms of venom allergy in individuals. Here the authors examine the underlying immune cell changes after venom specific AIT in early time points after therapy initiation showing indicative changes in specific immune cell populations. [ABSTRACT FROM AUTHOR]

Distributed cooperative control strategies for DC microgrids have been rapidly evolving in recent years. However, introducing a cyber layer to enhance robustness, scalability, and reliability also exposes the system to potential cyber‐attacks. The damage inflicted by such attacks on the system performance can be catastrophic, reaching a point where it may devastate the system normal operation. By using model reference adaptive control (MRAC), this article proposes a resilient approach that does not require an accurate model of the system and despite the uncertainties for detecting false data injections into the reference DC voltage and simultaneously mitigating their adverse effects on the system stability and performance. The proposed technique employs an observer to detect possible false data injections in an online manner. By emulating the behavior of an ideal reference model, the MRAC ensures adaptive adjustments of the control parameters over time to mitigate the negative effects of potential attacks effectively and despite non‐idealities such as measurement noise, parameter variations, and environmental changes in DC microgrids, the MRAC effectively manages false data injection attacks. Simulation studies are conducted using diverse scenarios involving a three‐node DC microgrid to show the effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

Key Clinical Message: We report a patient who sustained an AFF (Atypical femoral fractures) after 15 years of bisphosphonate treatment. DEXA scans can be utilized to identify early evidence of AFF in asymptomatic patients up to 9 years before the development of an AFF. [ABSTRACT FROM AUTHOR]

Context. Magnetic turbulence is classified as weak or strong based on the relative amplitude of the magnetic field fluctuations compared to the mean field. These two classes have different energy transport properties. Aims. The purpose of this study is to analyze turbulence in the interstellar medium (ISM) based on this classification. Specifically, we examined the ISM of simulated galaxies to detect evidence of strong magnetic turbulence and provide statistics on the associated magnetic coherent structures (MCoSs), such as current sheets, that arise in this context. Methods. We analyzed magnetohydrodynamic galaxy simulations with different initial magnetic field structures (either completely ordered or completely random) and recorded statistics on the magnetic field fluctuations (δB/B0) and the MCoSs, which are defined here as regions where the current density surpasses a certain threshold. We also studied the MCoS sizes and kinematics. Results. The magnetic field disturbances in both models follow a log-normal distribution, peaking at values close to unity; this distribution turns into a power law at large values (δB/B0 > 1), which is consistent with strong magnetic turbulence The current densities are widely distributed, with non-power-law deviations from a log-normal at the largest values. These deviating values of the current density define MCoSs. We find that, in both models, MCoSs are fractally distributed in space, with a typical volume-filling factor of about 10%, and tend to coincide with peaks of star formation density. Their fractal dimension is close to unity on sub-kiloparsec scales, and between 2 and 3 on larger scales. These values are consistent with MCoSs having a sheet-like or filament-like morphology. Conclusions. Our work challenges the prevailing paradigm of weak magnetic turbulence in the ISM by demonstrating that strong magnetic disturbances can occur even when the initial magnetic field is completely ordered. This strong magnetic turbulence arises self-consistently from differential rotation and supernova feedback. Our findings provide a foundation for a magnetic turbulence description of the galactic ISM that includes strong fluctuations of the magnetic field. [ABSTRACT FROM AUTHOR]

Purpose: National Clinical Guidelines recommend an integrated combination of conservative management strategies for sciatica. However, the efficacy of such combinations have not been established. The purpose of this systemic review with meta-analysis was to determine the efficacy of combined conservative (non-pharmacological) compared to single interventions for people with sciatica with a confirmed neuropathic mechanism. Methods: The systematic review was registered on PROSPERO CRD42023464011. The databases included were the Cochrane Central Register of Controlled Trials (CENTRAL), CINAHL (EBSCO), Embase, PubMed, Scopus, APA PsycINFO, and grey literature sources from inception until January 2024. Inclusion criteria were randomized controlled trials that assessed the effectiveness of combined non-pharmacological interventions in comparison to a control intervention among individuals with sciatica of a neuropathic origin identified using diagnostic or clinical tests. Primary outcomes were back pain, leg pain, and disability. The secondary outcome was global rating of change. Study selection, data extraction and risk of bias assessment (using Cochrane ROB2) were assessed by two reviewers. Meta-analysis was performed with a random effects model with inverse variance weighting used for the metanalysis using SPSS v 29. Results: 3,370 articles were identified, of which 6 were included. Risk of bias was high in one study and had some concerns in the remaining 5 studies for each outcome measure. There was evidence of efficacy for combined interventions for back pain in the short-and long-term (SMD − 0.56 (95% CI -0.91, -0.22, p = 0.01, I2 = 0.2; SMD − 0.44 (95% CI -0.79, -0.1, p = 0.03, I2 = 0.00), and for disability in the short term (SMD − 0.48 (95% CI -0.92, -0.04, p = 0.04, I2 = 0.72). There was no evidence of efficacy for leg pain at any time point ((short term SMD − 0.45 (95% CI -0.91, 0.02, p = 0.06, I2 = 0.65), medium term (SMD − 0.29 (95% CI -1.12, 0.54, p = 0.35, I2 = 0.82), long term (SMD − 0.40 (95% CI -1.23, 0.44, p = 0.18, I2 = 0.57).Certainty of evidence ranged from very low to moderate. Conclusion: There are few studies that have combined conservative (non-pharmacological) interventions for the management of sciatica with a neuropathic component pain mechanism, as recommended by National Clinical Guidelines. This review indicates that combining conservative (no-pharmacological) management strategies appeared more effective than single interventions for the outcomes of low back pain in the short and long term, and for disability in the short term, but not for leg pain at any time point. The overall low certainty of evidence, suggests that future studies with more robust methodologies are needed. [ABSTRACT FROM AUTHOR]

Purpose: Trial data shows modest reductions in leg pain, disability and surgery avoidance following epidural steroid injections (ESI) for severe sciatica. Despite their common use, there is no clear evidence about which patients are more likely to benefit from ESI. The aim of this study was to generate consensus on potential predictors of outcome following ESI for disc-related sciatica. Methods: A list of potential predictors of outcome was generated during a consensus meeting of seven experts. The items were subsequently presented in a two round on-line Delphi study to generate consensus among experts on which items are potential predictors of outcome. Consensus was defined as 70% agreement among participants. Results: Sixty-one items were generated during the consensus meeting. Of ninety experts invited to participate in the on-line Delphi study, 44 (48%) and 33 (73%) took part in rounds one and two respectively. Twenty-eight additional items suggested by participants in round one were included in round two. Overall, 14 items reached consensus reflecting domains of health, medication use, pain intensity, psychosocial factors, imaging findings and type of injection. Conclusion: Based on expert consensus, items that can be routinely collected in clinical practice were identified as potential predictors of outcomes following ESI. [ABSTRACT FROM AUTHOR]

Introduction: Relapsing-Remitting Multiple Sclerosis manifests various motor symptoms including impairments in corticospinal tract integrity, whose symptoms can be assessed using transcranial magnetic stimulation. Several factors, such as exercise and interlimb coordination, can influence the plastic changes in corticospinal tract. Previous work in healthy and chronic stroke survivors showed that the greatest improvement in corticospinal plasticity occurred during in-phase bilateral exercises of the upper limbs. Altered corticospinal plasticity due to bilateral lesions in the central nervous system is common after Multiple Sclerosis, yet the effect of in-phase bilateral exercise on the bilateral corticospinal plasticity in this cohort remains unclear. Our aim was to investigate the effects of in-phase bilateral exercises on central motor conduction time, motor evoked potential amplitude and latency, motor threshold and clinical measures in people with Relapsing-Remitting Multiple Sclerosis. Methods: Five people were randomized and recruited in this single case concurrent multiple baseline design study. The intervention protocol lasted for 12 consecutive weeks (30–60 minutes /session x 3 sessions / week) and included in-phase bilateral upper limb movements, adapted to different sports activities and to functional motor training. To define the functional relation between the intervention and the results, we conducted a visual analysis. If a potential sizeable effect was observed, we subsequently performed a statistical analysis. Results: Results demonstrated bilateral reduction of the motor threshold alongside with improvement of all clinical measures, but not in any other corticospinal plasticity measures. Conclusion: Our preliminary findings suggest that in-phase bilateral exercise affects motor threshold in people with Relapsing-Remitting Multiple Sclerosis. Therefore, this measure could potentially serve as a proxy for detecting corticospinal plasticity in this cohort. However, future studies with larger sample sizes should validate and potentially establish the effect of in-phase bilateral exercise on the corticospinal plasticity and clinical measures in this cohort. Trial registration: Clinical trial registration: ClinicalTrials.gov NCT05367947. [ABSTRACT FROM AUTHOR]

Ischemic stroke (IS) is a severe complication and leading cause of mortality in patients under extracorporeal membrane oxygenation (ECMO). The aim of our narrative review is to summarize the existing evidence and provide a deep examination of the diagnosis and treatment of acute ischemic stroke patients undergoing ECMO support. The incidence rate of ISs is estimated to be between 1 and 8%, while the mortality rate ranges from 44 to 76%, depending on several factors, including ECMO type, duration of support and patient characteristics. Several mechanisms leading to ISs during ECMO have been identified, with thromboembolic events and cerebral hypoperfusion being the most common causes. However, considering that most of the ECMO patients are severely ill or under sedation, stroke symptoms are often underdiagnosed. Multimodal monitoring and daily clinical assessment could be useful preventive techniques. Early recognition of neurological deficits is of paramount importance for prompt therapeutic interventions. All ECMO patients with suspected strokes should immediately receive brain computed tomography (CT) and CT angiography (CTA) for the identification of large vessel occlusion (LVO) and assessment of collateral blood flow. CT perfusion (CTP) can further assist in the detection of viable tissue (penumbra), especially in cases of strokes of unknown onset. Catheter angiography is required to confirm LVO detected on CTA. Intravenous thrombolytic therapy is usually contraindicated in ECMO as most patients are on active anticoagulation treatment. Therefore, mechanical thrombectomy is the preferred treatment option in cases where there is evidence of LVO. The choice of the arterial vascular access used to perform mechanical thrombectomy should be discussed between interventional radiologists and an ECMO team. Anticoagulation management during the acute phase of IS should be individualized after the thromboembolic risk has been carefully balanced against hemorrhagic risk. A multidisciplinary approach is essential for the optimal management of ISs in patients treated with ECMO. [ABSTRACT FROM AUTHOR]

Background/Objectives: Vaccination of pregnant and postpartum women for pertussis, influenza and COVID-19 not only protects themselves but also offspring. Despite the benefits of this approach, vaccination uptake remains suboptimal in pregnancy. Where the opportunity to be vaccinated in pregnancy is missed, the offer of vaccination in the post-partum period may be an alternative strategy. The aim of this systematic review is to assess the impact of interventions to increase vaccination uptake in the postpartum period on vaccination uptake. Methods: A literature search was performed in MEDLINE, including interventional studies promoting vaccination uptake in postpartum women published between 2009 and 2024. The search was conducted according to PRISMA guidelines and registered with PROSPERO. Results: We finally included 16 studies in the review, and the primary outcome was vaccination uptake in the postpartum period. The most significant factors for increasing uptake were recommendation from healthcare providers, type of interventions used, and delivery of vaccines in the maternity wards or the community. Conclusions: In conclusion, maternal vaccination rates in the postpartum period may increase with targeted education by healthcare professionals and positive reinforcement. The interventions described in these studies could be applied in the healthcare systems worldwide. [ABSTRACT FROM AUTHOR]

Microbially induced carbonate precipitation (MICP) represents a technique for biocementation, altering the hydraulic and mechanical properties of porous materials using bacterial and cementation solutions. The efficacy of MICP depends on various biochemical and environmental elements, requiring careful consideration to achieve optimal designs for specific purposes. This study evaluates the efficiency of different MICP protocols under varying environmental conditions, employing two bacterial strains: S. pasteurii and S. aquimarina, to optimize soil strength enhancement. In addition, microscale properties of carbonate crystals were investigated and their effects on soil strength enhancement were analyzed. Results demonstrate that among the factors investigated, bacterial strain and concentration of cementation solution significantly influence the biochemical aspect, while temperature predominantly affects the environmental aspect. During the MICP treatment process, the efficiency of chemical conversion through S. pasteurii varied between approximately 80% and 40%, while for S. aquimarina, it was only around 20%. Consequently, the CaCO3 content resulting from MICP treatment using S. pasteurii was significantly higher, ranging between 5% and 7%, compared to that achieved with S. aquimarina, which was about 0.5% to 1.5%. The concentration of the cementation solution also plays a pivotal role, with an optimized value of 0.5 M being critical for achieving maximum efficiency and CaCO3 content. The ideal temperature span for MICP operation falls between 20°C and 35°C, with salinity and oxygen levels exerting minor impact. Furthermore, although salinity influences the characteristics of formed carbonate crystals, its effect on unconfined compressive strength (UCS) values of MICP-treated soil remains marginal. Samples subjected to a one-phase treatment, adjusted to pH values between 6.0 and 7.5, exhibit roughly half the UCS strength compared to the two-phase treatment. These findings hold promising potential for MICP applications in both terrestrial and marine environments for strength enhancement. [ABSTRACT FROM AUTHOR]

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Purpose: Crayfish treatment leads to the production of waste and byproducts up to 80% of the total initial seafood amount. The current common method to handle such waste is the landfilling practice which produces remarkable amounts of hazardous gasses and substances due to the anaerobic decomposition process. In this study, an integrated procedure is proposed for the valorization of wastes produced by a fish and crustaceous enterprise. Methods: According to the circular economy and environmentally friendly practices spirit, integrated vertical production and waste valorization methods are adopted globally to reduce food waste. In this study, a process consisting of four sequential steps was proposed to valorize crayfish-treatment waste. Four final added-value materials were produced during this study. The first was chitosan which was identified via the NMR technique exhibiting 91% deacetylation degree. The other two were calcium hydroxide particles of size 6.32 μm and calcium carbonate particles of size 16.9 μm exhibiting meso-macro-pore structure with Sg = 46m2/g and Sg = 20m2/g respectively. Pore specific surface area values of these two products are similar to values reported in the literature for applications such as drug carriers etc. The last product was nitrogen/sulfur-doped carbon quantum dots which exhibited an identified via AFM technique mean size of 5.01 nm. This material was successfully tested for its advanced antioxidant capabilities via the DPPH assay and for its antimicrobial properties via MIC routine measurements with E. coli and L. monocytogenes. Results: Experimental data indicated that the valorization process proposed in this study could lead to a yield of 10% w/w for chitosan after three stages treatment, to a yield of 35% w/w for Ca(OH)2 or CaCO3 after 1 stage treatment, and to a yield of 5% w/w N-S co-dopped Carbon QDs. Conclusion: The globally big amount of waste-shells from Crayfish and other crustaceous could be integratedly exploited for added-value materials production yielding economic and environmental benefits. Highlights: Crayfish waste shells are valorized. Integrated valorization of waste shells of crustaceous is feasible. Calcium carbonate and Calcium hydroxide powders exhibiting very good properties for different applications could be produced from waste shells valorization. High added value materials such as chitin and chitosan biopolymers could be produced from waste shells valorization. Advanced quantum dots such as nitrogen/sulfur dopped could be produced from waste shells valorization. [ABSTRACT FROM AUTHOR]

Simple Summary: The skin is the outermost barrier of the human body and consists of different layers and cell types. Several environmental and genetic factors can induce skin aging and age-related diseases. One of the main problems in skin aging is that senescent cells are accumulated and secrete factors, which can induce senescence in other tissues. Many researchers are trying to identify treatment modalities (known as senotherapies) to eliminate the senescent cells and reverse the aging process for chronic age-related diseases. The aim of this study is to address the mechanisms that induce senescence and the molecules with potential HAFi effects that are currently investigated for skin aging. Further studies should be conducted to elucidate all the effects of current senotherapies on the skin and other organs. Current data suggest that ongoing research projects in the field may lead to the discovery of new effective anti-senescence strategies in the skin. The skin is the layer of tissue that covers the largest part of the body in vertebrates, and its main function is to act as a protective barrier against external environmental factors, such as microorganisms, ultraviolet light and mechanical damage. Due to its important function, investigating the factors that lead to skin aging and age-related diseases, as well as understanding the biology of this process, is of high importance. Indeed, it has been reported that several external and internal stressors contribute to skin aging, similar to the aging of other tissues. Moreover, during aging, senescent cells accumulate in the skin and express senescence-associated factors, which act in a paracrine manner on neighboring healthy cells and tissues. In this review, we will present the factors that lead to skin aging and cellular senescence, as well as ways to study senescence in vitro and in vivo. We will further discuss the adverse effects of the accumulation of chronic senescent cells and therapeutic agents and tools to selectively target and eliminate them. [ABSTRACT FROM AUTHOR]

Purpose: To report a case of bilateral exudative retinal detachments and panuveitis in a patient with multiple myeloma (MM). Case Report: A 54-year-old patient with non-proliferative diabetic retinopathy was referred with blurred vision and scotomas in both eyes (OU). Three months prior to the onset of ocular symptoms, he was diagnosed with systemic MM and was receiving chemotherapy. Clinical examination revealed best-corrected visual acuities of 20/80 OU, rare anterior chamber cell, 2+ vitreous cell, diffuse intraretinal hemorrhages, and exudative retinal detachments (RD). Optical coherence tomography of the macula showed central subretinal fluid with cystic intraretinal fluid OU. The findings were consistent with panuveitis and exudative RD in the setting of MM. He reported symptomatic improvement after plasmapheresis and oral prednisone initiation. Conclusion: Extensive, bilateral exudative RD and panuveitis are rare but potentially sight-threatening findings in patients with MM. [ABSTRACT FROM AUTHOR]

Introduction: Reconstructing long bone defects in the upper limbs, particularly the radius and ulna, poses significant challenges. These defects, resulting from trauma, tumors, infections, or congenital anomalies, require precise surgical intervention for functional restoration. Traditional non-vascularized autogenous bone grafts have limitations, such as resorption and limited biological activity. To address these challenges, free vascularized fibular grafts (FVFGs) have been developed, offering enhanced recovery by supplying nutrients and structural support, particularly in large defects or compromised vascularity. Materials and Methods: This retrospective study reviewed patients with significant forearm skeletal defects treated with FVFGs at our institution from January 2008 to January 2019. Included were patients with radius or ulna defects exceeding 8 cm due to trauma, tumor excision, or non-union fractures. Data on demographics, clinical details, surgical techniques, and outcomes—including graft union time, complications, range of motion, and the disabilities of the arm, shoulder and hand (DASH) scores—were analyzed. Results: Eight patients, with a mean age of 27.6 years and an average defect length of 9.8 cm, were included. All patients achieved graft union within an average of 4 months, with no tumor recurrence or significant complications. Functional outcomes showed mean forearm pronation of 56.9 degrees, supination of 52.5 degrees, and a mean DASH score of 17.7. Conclusions: FVFG is a safe and effective technique for managing complex forearm bone defects, providing high union rates and good functional outcomes. It should be considered a primary option for large forearm skeletal defects. [ABSTRACT FROM AUTHOR]

The article focuses on the surrealist conception of imagination, particularly through the works of André Breton and Czech surrealists like Jan Švankmajer. It emphasizes Breton's call to revive the imagination as a means of liberation and explores how Czech theorists, especially Vratislav Effenberger, expanded upon his ideas. It argues that surrealism serves as a blueprint for understanding the transformative power of imagination in the human experience.

Post-traumatic cerebral venous sinus thrombosis (ptCVST) often remains underdiagnosed due to the non-specific nature of clinical signs, commonly mimicking severe traumatic brain injury (TBI) manifestations. Early recognition of this rare and potentially life-threatening complication is crucial for the effective management of severe TBI patients in Intensive Care. The present study reports the case of a 66-year-old male who was transferred to the emergency department due to moderate TBI. Initial emergency brain computed tomography (CT) scans revealed certain traumatic lesions, not necessitating any urgent neurosurgical intervention. During his stay in an Intensive Care Unit (ICU), multiple transient episodes of intracranial pressure (ICP) values were managed conservatively, and through placement of an external ventricular drain. Following a series of CT scans, there was a continuous improvement of the initial traumatic hemorrhagic findings despite his worsening clinical condition. This paradox raised suspicion for ptCVST, and a brain CT venography (CTV) was carried out, which showed venous sinus thrombosis close to a concomitant skull fracture. Therapeutic anticoagulant treatment was administered. The patient was discharged with an excellent neurological status. To date, there are no clearly defined guidelines for medical and/or surgical management of patients presenting with ptCVST. Therapy is mainly based on intracranial hypertension control and the maintenance of normal cerebral perfusion pressure (CCP) in the ICU. The mismatch between clinical and imaging findings in patients with TBI and certain risk factors raises the suspicion of ptCVST. [ABSTRACT FROM AUTHOR]

Medication adherence (MA) to recommended treatment is a multi-faceted problem and an ongoing challenge for healthcare providers (HCPs) to monitor. This qualitative study with 10 HCPs in Cyprus aims to explore HCPs' perceptions and strategies used on addressing medication non-adherence (MNA) in patients with chronic conditions. Two main themes emerged from the analysis reflecting the ways that HCPs described their reactions to MNA of their patients: (1) "Relying on information provision to improve MA" and (2) "Trying to understand patients' perspective." HCPs reported empathizing with patients and engaging in discussions to understand patients' perspective and reasons for MNA, so as to explore alternative solutions. Simultaneously, some HCPs reflected that the techniques used to improve MA are solely centered around information on medication and side-effects. HCPs experienced an internal conflict between providing patient-centered care versus using directive approaches to improve MA. Findings suggest how HCPs could thoroughly address patients' individual barriers. [ABSTRACT FROM AUTHOR]

Objectives: Use of intracoronary imaging (ICI) in cases of stent thrombosis (ST) is recommended and tailored treatment appears reasonable. Nevertheless, data supporting such a strategy are lacking. The aim of this study was to evaluate the clinical impact of ICI in the management of ST. Methods: The unadjusted study population was consecutive patients with definite ST presenting in a single tertiary cardiac centre and undergoing percutaneous coronary intervention (PCI). The presumed major mechanism of ST was assigned according to the real-time ICI interpretation by the PCI operator. Propensity score matching was performed with regard to ICI use to form the adjusted population and Kaplan–Meier analysis was applied to compare survival free of cardiac death (CD) or target lesion revascularization (TLR). Results: The unadjusted population included 130 ST patients, with the majority presenting with ST-elevation myocardial infarction (STEMI) (88%) and very late ST (86%). ICI was performed in 45 patients, of whom optical coherence tomography (OCT) was performed in 30 cases. When the individual ST mechanisms were viewed as groups, there was an interaction observed between type of treatment (stent vs. non-stent) and ST mechanism, with non-stent treatment being more prevalent in cases of underexpansion, malapposition, in-stent restenosis and mechanism uncertainty. After application of matching, two groups of 30 patients were formed. ICI-guided management resulted in better survival free of CD–TLR at 2 years (93% vs. 73%, p = 0.037). Conclusions: Intracoronary imaging guidance during PCI for ST had a direct impact on management (stent vs. non-stent) and resulted in a lower event rate at mid-term follow-up when propensity matched analysis was applied. [ABSTRACT FROM AUTHOR]

Brain computation performed by billions of nerve cells relies on a sufficient and uninterrupted nutrient and oxygen supply1,2. Astrocytes, the ubiquitous glial neighbours of neurons, govern brain glucose uptake and metabolism3,4, but the exact mechanisms of metabolic coupling between neurons and astrocytes that ensure on-demand support of neuronal energy needs are not fully understood5,6. Here we show, using experimental in vitro and in vivo animal models, that neuronal activity-dependent metabolic activation of astrocytes is mediated by neuromodulator adenosine acting on astrocytic A2B receptors. Stimulation of A2B receptors recruits the canonical cyclic adenosine 3′,5′-monophosphate–protein kinase A signalling pathway, leading to rapid activation of astrocyte glucose metabolism and the release of lactate, which supplements the extracellular pool of readily available energy substrates. Experimental mouse models involving conditional deletion of the gene encoding A2B receptors in astrocytes showed that adenosine-mediated metabolic signalling is essential for maintaining synaptic function, especially under conditions of high energy demand or reduced energy supply. Knockdown of A2B receptor expression in astrocytes led to a major reprogramming of brain energy metabolism, prevented synaptic plasticity in the hippocampus, severely impaired recognition memory and disrupted sleep. These data identify the adenosine A2B receptor as an astrocytic sensor of neuronal activity and show that cAMP signalling in astrocytes tunes brain energy metabolism to support its fundamental functions such as sleep and memory.This study explores how adenosine A2B receptors can act as astrocytic sensors of brain metabolic activity and how cAMP signalling in astrocytes may support core brain functions such as sleep and memory. [ABSTRACT FROM AUTHOR]

This work demonstrates that the packaging material commonly called "bubble wraps" (Aeroplast) can be leveraged to serve as a probe to visible sunlight exposure. This probe relies on a newly developed layered metal selenide photocatalyst with the general formula (DMAH)2xMnxSn3–xSe6 (DMSe‐1) (x = 1.3–1.7; DMAH+ = dimethylammonium), featuring a narrow bandgap of 0.76 eV, in addition to an indicator dye and a reducing agent. The photochemically sensitive probe is introduced into the air‐filled compartments of bubble wraps and undergoes photocatalytic degradation, resulting in a chromatic response to sunlight exposure. The probe's sensitivity to variable irradiation doses can be adjusted by varying the amount of the photocatalyst. The color intensity correlates with the absorbed irradiation dose, allowing for qualitative assessment by the naked eye or quantitative measurement using the RGB color system. The results obtained from the new probe agree with those obtained from standard sunlight pyranometers (r = 0.98), with an average error of <15%. This suggests that beyond their use as protective coatings, bubble wraps can be successfully repurposed as visible light sensors. Furthermore, this study describes the initial use of metal chalcogenides as visible light probes, potentially paving the way for the development of innovative light‐sensitive materials. [ABSTRACT FROM AUTHOR]

In Europe, the largest and most floristically diverse ultramafic (serpentine) outcrops are located on the Balkan Peninsula. In Greece, the Northern Pindus mountain range is characterized as a floristic diversity hotspot, due to the presence of large ultramafic outcrops that harbor a large number of nickel (Ni) hyperaccumulators with potential use in agromining. The work presented here is part of a more in‐depth study of floristic composition and ecology of plant communities in ultramafic grasslands of the Northern Pindus mountain range. In 2021–2022, 120 relevés in three subareas (sA1, sA2, and sA3) were sampled according to the Braun‐Blanquet method. We identified 493 plant taxa belonging to 61 families. The most abundant families were Asteraceae, Poaceae, Fabaceae, Lamiaceae, and Caryophyllaceae. A total of 22 plant taxa are endemic to Greece. Ninety‐five taxa are of Balkan origin while 158 taxa are distributed to the broader Mediterranean region. The highest number of plant life‐forms are hemicryptophytes (56.39%) and therophytes (23.33%). Eight Ni hyperaccumulators including Centaurea thracica, Bornmuellera baldaccii, Bornmuellera emarginata, Bornmuellera tymphaea, Noccaea boeotica, Noccaea tymphaea, Odontarrhena chalcidica, and Odontarrhena smolikana were recorded. [ABSTRACT FROM AUTHOR]

The nickel hyperaccumulator Bornmuellera tymphaea has been evaluated for use in agromining in Greece with very promising results. This study examines propagation of this species based on cuttings, which provides rapid multiplication and clonal propagation of genotypes with useful genetic traits and offers obvious advantages over propagation by seed. Apical cuttings, 10 cm long, obtained from plants in the field, were propagated on mist benches under greenhouse conditions. Four treatments based on the rooting medium were evaluated: (a) perlite, (b) perlite + peat 1:1 (v/v), (c) ultramafic soil, and (d) ultramafic soil + perlite 1:1 (v/v). Completely randomized blocks were used in a 4 × 2 factorial design, using 4 different propagation rooting media and 2 levels of auxins (with or without the use of indole‐3‐butyric acid [IBA]), with 3 replicates of 28 cuttings. The response to perlite medium was best in terms of rooting: 75% without IBA and 71.33% with IBA, and the dry weight of roots was 0.18 g and of shoots 0.34 g. Perlite + peat 1:1 (v/v) medium also resulted in rooting in more than 55% of cuttings whereas cuttings grown in ultramafic soil showed very low rooting (<27%). Based on the findings, B. tymphaea has an aptitude for vegetative propagation by cuttings with survival values and rooting greater than 70%. Perlite combined with the application of auxin (ΙΒΑ) is recommended. [ABSTRACT FROM AUTHOR]

The Greek endemic Bornmuellera emarginata, known for its Ni hyperaccumulation capacity, is a promising candidate species for use in agromining. At the same time, the installation of photovoltaic systems on agricultural land has recently been increasing. Field observations that populations of this species usually occupy shaded places under shrubs or trees led us to study the effect of different shading levels on the growth of B. emarginata, with a view to combine the cultivation of the species with photovoltaic panels in the same area. The experiment was conducted under greenhouse conditions and lasted 210 days after sowing (DAS). Seeds were sown directly into 2‐L pots filled with ultramafic soil collected from the field. Three plant samplings (81, 140, and 210 DAS) were conducted during the experiment to determine a set of morphological and physiological parameters of the species (fresh and dry plant biomass, plant height, number of lateral shoots, total number of leaves, proline and chlorophyll concentrations) alongside with its nickel yield at different levels of shading (0%, 20%, 35%, 60%, and 90%). Growth of B. emarginata was only significantly affected at the highest shading level. Nickel yield of the species increased over time and was maximized at the end of the experiment. The highest nickel yield per plant was observed at the intermediate shading level (35%). Bornmuellera emarginata can be considered as a candidate nickel hyperaccumulator for cultivation in ultramafic or nickel‐polluted areas under photovoltaic panels with corresponding shading, providing important environmental benefits. [ABSTRACT FROM AUTHOR]

This systematic review and meta‐analysis aimed to consolidate evidence on dietary interventions for atopic eczema/dermatitis (AD) skin symptoms in children without food allergies, following PRISMA 2020 guidelines. Systematic review updates were conducted in May 2022 and June 2023, focusing on randomized placebo‐controlled trials (RCTs) involving children with AD but without food allergies. Specific diets or supplements, such as vitamins, minerals, probiotics, prebiotics, symbiotics, or postbiotics, were explored in these trials. Exclusions comprised descriptive studies, systematic reviews, meta‐analyses, letters, case reports, studies involving elimination diets, and those reporting on food allergens in children and adolescents. Additionally, studies assessing exacerbation of AD due to food allergy/sensitization and those evaluating elimination diets' effects on AD were excluded. Nutritional supplementation studies were eligible regardless of sensitization profile. Evaluation of their impact on AD clinical expression was performed using SCORAD scores, and a meta‐analysis of SCORAD outcomes was conducted using random‐effect models (CRD42022328702). The review encompassed 27 RCTs examining prebiotics, Vitamin D, evening primrose oil, and substituting cow's milk formula with partially hydrolyzed whey milk formula. A meta‐analysis of 20 RCTs assessing probiotics, alone or combined with prebiotics, revealed a significant reduction in SCORAD scores, suggesting a consistent trend in alleviating AD symptoms in children without food allergies. Nonetheless, evidence for other dietary interventions remains limited, underscoring the necessity for well‐designed intervention studies targeting multiple factors to understand etiological interactions and propose reliable manipulation strategies. [ABSTRACT FROM AUTHOR]

Acute respiratory infections are a major cause of morbidity and mortality in children worldwide. Dietary and nutritional interventions, including minerals and vitamin supplementation, have been explored as potential treatments for these infections. However, the evidence on their efficacy is limited and inconclusive. This systematic review and meta‐analysis aim to provide a comprehensive summary of the available evidence on the effectiveness of dietary and nutritional interventions for treating acute respiratory tract infections in children. A systematic review was conducted according to the PRISMA 2020 guidelines in April 2022 and updated in April 2023. Clinical trials focusing on dietary or nutritional interventions, including supplementations, in children with acute respiratory tract infections were included. The selection of interventions and outcomes was based on biological plausibility. Data were extracted using a standardized form, and the risk of bias was assessed using the Cochrane Risk of Bias Tool. Meta‐analysis was performed using random‐effect models. A total of 50 studies were included in the review. Four trials were conducted in low, 32 in lower‐middle, 12 in upper‐middle, and only two in high‐income countries. The studies evaluated various dietary interventions, including zinc, vitamin A, vitamin E, vitamin D, and probiotics. The results of individual studies on the efficacy of these interventions were mixed, with some showing positive effects on clinical outcomes such as duration of symptoms, while others showed no significant impact. Meta‐analysis was conducted for zinc supplementation in children with pneumonia, and the pooled results suggested a potential limited benefit in terms of reduced hospital length of stay but not time to recovery. Meta‐analyses on vitamin D did not show any effect in children with pneumonia. This systematic review fills a critical gap in the literature by synthesizing the available evidence on the efficacy and safety of nutritional or dietary interventions for acute respiratory tract infections in children. The findings indicate no dietary or nutritional intervention can currently be recommended for the routine treatment of respiratory tract infections in children based on single supplement studies. The metanalysis suggests that zinc supplementation might have a beneficial effect on length of hospitalization in children with pneumonia. New studies are needed to establish more conclusive evidence for pediatric acute respiratory diseases especially for children living in a context of high‐income countries. [ABSTRACT FROM AUTHOR]

Photocatalysis is a prominent alternative wastewater treatment technique that has the potential to completely degrade pesticides as well as other persistent organic pollutants, leading to detoxification of wastewater and thus paving the way for its efficient reuse. In addition to the more conventional photocatalysts (e.g., TiO2, ZnO, etc.) that utilize only UV light for activation, the interest of the scientific community has recently focused on the development and application of visible light-activated photocatalysts like g-C3N4. However, some disadvantages of g-C3N4, such as the high recombination rate of photogenerated charges, limit its utility. In this light, the present study focuses on the synthesis of WO3 fibers/g-C3N4 Z-scheme heterojunctions to improve the efficiency of g-C3N4 towards the photocatalytic removal of the widely used insecticide clothianidin. The effect of two different g-C3N4 precursors (urea and thiourea) and of WO3 fiber content on the properties of the synthesized composite materials was also investigated. All aforementioned materials were characterized by a number of techniques (XRD, SEM-EDS, ATR-FTIR, Raman spectroscopy, DRS, etc.). According to the results, mixing 6.5% W/W WO3 fibers with either urea or thiourea derived g-C3N4 significantly increased the photocatalytic activity of the resulting composites compared to the precursor materials. In order to further elucidate the effect of the most efficient composite photocatalyst in the degradation of clothianidin, the generated transformation products were tentatively identified through UHPLC tandem high-resolution mass spectroscopy. Finally, the detoxification effect of the most efficient process was also assessed by combining the results of an in-vitro methodology and the predictions of two in-silico tools. [ABSTRACT FROM AUTHOR]

This document provides a commentary on a paper titled "Marked Spatial Point Processes: Current State and Extensions to Point Processes on Linear Networks." The paper summarizes the current state of the field and proposes extensions to marked linear network point processes. It focuses on reviewing summary statistics for point processes and discusses other important topics in the field, such as marked temporal point processes and the Growth-Interaction process. The document explains different mark spaces, summary statistics, and product density/intensity functions for marked point processes. It also discusses concepts like mark filtering, marked intensity reweighted stationarity, kth-order intensity reweighted stationarity, and mark-weighted kth-order summary statistics. The text explores the use of test functions and weighted local summary statistics, as well as linear network summary statistics and marked inhomogeneous summary statistics. The authors propose proper estimators for these summary statistics and discuss their applications. [Extracted from the article]

This paper presents the main features of the Greek political landscape during the period 1974–2008 and examines whether the governing ideology of the country's main political parties is correlated with specific fiscal and monetary policy patterns. The descriptive analysis reveals two key findings. First, the data indicates ideological differences in fiscal policy during the pre-1993 period, with PASOK governments exhibiting higher public spending and deficits, while the conservative New Democracy (ND) demonstrated greater fiscal restraint. Monetary policy during this period also appears to follow ideological differences. Second, in the post-1993 period, two trends emerged that do not seem to be ideologically driven. From 1994 to 2001, both major political parties in Greece focused on joining the Economic and Monetary Union, aligning their policies with the Maastricht Treaty. This resulted in a decrease in government expenditure and an increase in total revenue, even under the left-wing PASOK government that was in office during that period. However, after Greece joined the eurozone, external constraints such as the Stability and Growth Pact did not effectively enforce fiscal discipline. From 2001 onward, there was an increase in expansionary fiscal policies, even under the right-wing ND government, suggesting that government ideology had minimal influence on fiscal variables during this period. Greece provides an intriguing example of how partisan politics can intersect with international institutional and policy constraints. (JEL codes: H2 and H1) [ABSTRACT FROM AUTHOR]

Bazhanov-Stroganov (4-simplex) maps are set-theoretical solutions to the 4-simplex equation, namely the fourth member of the family of n-simplex equations, which are fundamental equations of mathematical physics. In this paper, we develop a method for constructing Bazhanov-Stroganov maps as extensions of tetrahedron maps which are set-theoretical solutions to the Zamolodchikov tetrahedron (3-simplex) equation. We employ this method to construct birarional Bazhanov-Stroganov maps which boil down to the famous electric network and Hirota tetrahedron maps at a certain limit. [ABSTRACT FROM AUTHOR]

Hiking offers both recreational enjoyment and physical challenges, requiring speed adjustments when traversing uphill and downhill slopes. These adjustments prompt compensatory responses in kinematics and kinetics to mitigate fatigue and musculoskeletal strains. The study aimed to explore the impact of slope-specific walking speeds on spatiotemporal gait parameters, vertical ground reaction forces (vGRFs), and position of the center of pressure (COP) during uphill and downhill walking. Thirty-two healthy individuals completed five 4-min walks on an instrumented treadmill set to 0% (level), +10%, and +20% (uphill), and −10% and −20% (downhill), slopes, at 5.0, 3.5, 2.5, 5.0 and 3.5 km h−1, respectively. Uphill walking led to reduced stride length and cadence, increased foot rotation, step time, and durations of stance, swing, and double-stance phases. Conversely, downhill walking exhibited decreased step length, step time, and durations of stance, swing, and double-stance phases but increased step width and cadence compared to level walking. Speed adjustments to accommodate slope led to reduced vGRFs for uphill and downhill walking. Additionally, the COP shifted forward during uphill and backward during downhill walking and displaced laterally as walking became more demanding. The observed responses indicate adaptations aimed at maintaining postural control, reducing excessive load application, and optimizing energy expenditure on sloping terrain. [ABSTRACT FROM AUTHOR]

Data on antibody response (AR) after vaccination against SARS-CoV2 in hematopoietic stem-cell transplantation setting (HSCT) were initially scarce, mainly due to the exclusion of such patients from approval studies. Shortly after the worldwide application of vaccination against SARS-CoV-2 in vulnerable populations such as patients with hematologic malignancies, limited single-center trials, including HSCT patients, were published. However, there was a great heterogeneity between them regarding the type of underlying malignancy, co-current treatment, type of vaccine, method of AR measurement, and time point of AR measurement. Herein, we present the results of a prospective study on AR after vaccination for SARS-CoV-2 using the BNT162b2 vaccine in a cohort of 54 HSCT recipients—mostly autologous from a single Unit—along with a broad review of the current literature. In our cohort, the AR positivity rate at 1 month was 80.8% and remained positive in 85.7% of patients at 3 months after vaccination. There were only nine non-responders, who were more heavily pretreated and more frequently hypogammaglobulinemic compared to responders. High antibody titers (AT), [AT ≥ 1000 U/mL], were detected in 38.5% and 30.6% of the patients at m1 and m3, respectively. A significant decline in AT between m1 and m3 was demonstrated—p < 0.0001; median AT1 and AT3 were 480.5 and 293 U/mL, respectively. A novel finding of our study was the negative impact of IgA hypogammaglobulinemia on response to vaccination. Other negative significant factors were treatment with anti-CD20 antibody at vaccination and vaccination within 18 months from HSCT. Our data indicate that HSCT recipients elicit a positive response to the BNT162b2 vaccine against SARS-CoV-2 when vaccinated at 6 months post-transplant, and vaccination should be offered to this patient population even within the post-pandemic COVID-19 era. [ABSTRACT FROM AUTHOR]

Undoubtedly, the mental health of healthcare workers (HCWs) was negatively affected because of caring for patients during the COVID-19 pandemic. However, literature is limited on mapping the challenges and needs of HCWs during COVID-19 pandemic. A widely used framework in public health for mapping evidence includes the socio-ecological models, suggesting behavior can be influenced by individual, interpersonal, organizational, and community factors. The aim of this rapid scoping review was to use the socio-ecological model to map and compile lessons learnt from the literature regarding primarily the challenges and needs and secondly available psychological interventions for HCWs caring for COVID-19 patients. PubMed, CINAHL and Scopus databases were searched, with 21 studies finally included examining challenges and needs of HCWs and 18 studies presenting psychological interventions. Organizational-level challenges and needs such as inadequate staff preparation and supplies of protective equipment, flexible work policies and paid rest periods were the most reported. Individual-level challenges and needs included COVID-19-related fears and reduced mental health, whereas interpersonal-related needs included support provision. Community-level challenges included societal stigma. Certain psychological interventions were found to be promising for HCWs, but these were utilized to address only individual-level challenges and needs. Given that well-being entails an interaction of factors, multi-level interventions addressing multiple socio-ecological levels (interpersonal, organizational, community) and that place HCWs in their social context should be administrated to increase and maintain intervention' effects long-term and possibly aid in better coping with future pandemics. [ABSTRACT FROM AUTHOR]

The most common malignancy in women is breast cancer. During the development of cancer, oncogenic transcription factors facilitate the overproduction of inflammatory cytokines and cell adhesion molecules. Antiapoptotic proteins are markedly upregulated in cancer cells, which promotes tumor development, metastasis, and cell survival. Promising findings have been found in studies on the cell cycle-mediated apoptosis pathway for medication development and treatment. Dietary phytoconstituents have been studied in great detail for their potential to prevent cancer by triggering the body's defense mechanisms. The underlying mechanisms of action may be clarified by considering the role of polyphenols in important cancer signaling pathways. Phenolic acids, flavonoids, tannins, coumarins, lignans, lignins, naphthoquinones, anthraquinones, xanthones, and stilbenes are examples of natural chemicals that are being studied for potential anticancer drugs. These substances are also vital for signaling pathways. This review focuses on innovations in the study of polyphenol genistein's effects on breast cancer cells and presents integrated chemical biology methods to harness mechanisms of action for important therapeutic advances. [ABSTRACT FROM AUTHOR]

Background: Fragility-related pertrochanteric fractures have become a significant public health concern, with a rising incidence attributed to the expanding elderly demographic. Assessing patient-reported health-related quality of life (HRQoL), mortality, and factors correlated with them serves as a crucial metric in evaluating the effectiveness of hip fracture surgery. Methods: In a single-center retrospective study, 259 patients underwent surgical treatment with a cephalomedullary nail, with a mean follow-up of 21.7 months. Health-related quality of life (HRQoL) was assessed using SF-12 (12-item Short Form) and EQ-5D (EuroQoL-5 Dimensions) questionnaires. Mobility status was measured by the Crude Mobility Index (CMI). Surveys were administered during hospitalization and six months postoperatively. Statistical analysis involved descriptive statistics, non-parametric controls (Kendall, Mann-Whitney, and Wilcoxon), and Spearman correlation and logistic regression analysis, which were conducted using IBM SPSS version 28. Results: A statistically significant decrease was observed in the mean EQ-5D and SF-12 scores at 6 months post-op compared to the pre-fracture status. The ASA (American Society of Anaesthesiologists) score showed a significant correlation with the decrease in HRQoL measured by the SF-12 questionnaire. The 30-day post-operative mortality rate was 9.3%, increasing to 32.4% at 1 year. Notably, the 30-day mortality significantly rose during the pandemic era (5.0% vs. 12.0%; p = 0.003). Conclusions: Pertrochanteric hip fractures cause a lasting decline in quality of life. Annual mortality is high, and further investigations are needed to formulate policies that prevent hip fractures and reduce mortality rates. [ABSTRACT FROM AUTHOR]

As people age, they tend to spend more time indoors, and the colours in their surroundings may significantly impact their mood and overall well‐being. However, there is a lack of empirical evidence to provide informed guidance on colour choices, irrespective of age group. To work towards informed choices, we investigated whether the associations between colours and emotions observed in younger individuals also apply to older adults. We recruited 7393 participants, aged between 16 and 88 years and coming from 31 countries. Each participant associated 12 colour terms with 20 emotion concepts and rated the intensity of each associated emotion. Different age groups exhibited highly similar patterns of colour–emotion associations (average similarity coefficient of.97), with subtle yet meaningful age‐related differences. Adolescents associated the greatest number but the least positively biased emotions with colours. Older participants associated a smaller number but more intense and more positive emotions with all colour terms, displaying a positivity effect. Age also predicted arousal and power biases, varying by colour. Findings suggest parallels in colour–emotion associations between younger and older adults, with subtle but significant age‐related variations. Future studies should next assess whether colour–emotion associations reflect what people actually feel when exposed to colour. [ABSTRACT FROM AUTHOR]

Hereditary angioedema is a rare disease manifesting with recurrent attacks of disabling and potentially life-threatening angioedema, for which early diagnosis and effective therapy are critical. Recent scientific progress and the development of novel therapeutic options has engendered multiple important changes in the diagnosis and management of the disease. In an attempt to encourage and facilitate the use of this progress for all patients, the Hellenic Society of Angioedema has undertaken the effort to develop consensus recommendations for the diagnosis, treatment, and management of hereditary angioedema in special patients' groups. To this aim, a panel of 11 experts was assembled and a modified Delphi method was used. After a comprehensive review of relevant literature from the last five years retrieved from Medline, the initial text of the recommendations was formulated and consensus was sought among the experts. The consensus included statements that were agreed by more than 80% of the experts. This article presents the 24 consensus recommendations that were finally formulated. [ABSTRACT FROM AUTHOR]

BACKGROUND: The phoma stem canker pathogen Leptosphaeria maculans is one of the most widespread and devastating pathogens of oilseed rape (Brassica napus) in the world. Pathogen colonization is stopped by an interaction of a pathogen Avr effector gene with the corresponding host resistance (R) gene. While molecular mechanisms of this gene‐for‐gene interaction are being elucidated, understanding of effector function remains limited. The purpose of this study was to determine the action of L. maculans effector (AvrLm) genes on incompatible interactions triggered by B. napus noncorresponding R (Rlm) genes. Specifically, effects of AvrLm4‐7 and AvrLm1 on Rlm7‐mediated resistance were studied. RESULTS: Although there was no major effect on symptom expression, induction of defence genes (e.g. PR1) and accumulation of reactive oxygen species was reduced when B. napus cv. Excel carrying Rlm7 was challenged with a L. maculans isolate containing AvrLm1 and a point mutation in AvrLm4‐7 (AvrLm1, avrLm4‐AvrLm7) compared to an isolate lacking AvrLm1 (avrLm1, AvrLm4‐AvrLm7). AvrLm7‐containing isolates, isogenic for presence or absence of AvrLm1, elicited similar symptoms on hosts with or without Rlm7, confirming results obtained with more genetically diverse isolates. CONCLUSION: Careful phenotypic examination of isogenic L. maculans isolates and B. napus introgression lines demonstrated a lack of effect of AvrLm1 on Rlm7‐mediated resistance despite an apparent alteration of the Rlm7‐dependent defence response using more diverse fungal isolates with differences in AvrLm1 and AvrLm4. As deployment of Rlm7 resistance in crop cultivars increases, other effectors need to be monitored because they may alter the predominance of AvrLm7. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Spinal muscular atrophy (SMA) is a neurodegenerative disease caused by deficiency of the survival motor neuron (SMN) protein. Although SMA is a genetic disease, environmental factors contribute to disease progression. Common pathogen components such as lipopolysaccharides (LPS) are considered significant contributors to inflammation and have been associated with muscle atrophy, which is considered a hallmark of SMA. In this study, we used the SMNΔ7 experimental mouse model of SMA to scrutinize the effect of systemic LPS administration, a strong pro-inflammatory stimulus, on disease outcome. Systemic LPS administration promoted a reduction in SMN expression levels in CNS, peripheral lymphoid organs, and skeletal muscles. Moreover, peripheral tissues were more vulnerable to LPS-induced damage compared to CNS tissues. Furthermore, systemic LPS administration resulted in a profound increase in microglia and astrocytes with reactive phenotypes in the CNS of SMNΔ7 mice. In conclusion, we hereby show for the first time that systemic LPS administration, although it may not precipitate alterations in terms of deficits of motor functions in a mouse model of SMA, it may, however, lead to a reduction in the SMN protein expression levels in the skeletal muscles and the CNS, thus promoting synapse damage and glial cells' reactive phenotype. [ABSTRACT FROM AUTHOR]

This article discusses the implementation of a new GATE Digitizer Unit for versions post v9.3. The GATE Digitizer Unit is an important component of the Geant4 Application for Tomographic Emission (GATE), a platform used for numerical simulations in medical imaging and radiotherapy. The update was necessary to incorporate new features and address issues with the previous version. The article provides information on the added functionalities, impact on users, current status of the work, and future perspectives. The article also explains the new GDU framework and its architecture, as well as the testing conducted to ensure reliability and stability. The new GDU simplifies the architecture of the Digitizer Unit and allows for the digitization and output of multiple Sensitive Detectors. It provides flexibility and addresses limitations in previous versions of Geant4. The article discusses the use of the GATE software for simulation studies in medical imaging and presents simulation results comparing the old and new versions of GATE, showing good agreement between the two. The new version of GATE also offers improved execution speed and flexibility, allowing for the simulation of systems with multiple detector types and prototype experimental setups. The article highlights the potential uses of the new GDU and mentions future features planned for GATE. Overall, the GDU brings significant improvements to the GATE platform and further developments are ongoing. [Extracted from the article]

The personalized applications of 3D printing in interventional cardiology and cardiac surgery represent a transformative paradigm in the management of structural heart diseases. This review underscores the pivotal role of 3D printing in enhancing procedural precision, from preoperative planning to procedural simulation, particularly in valvular heart diseases, such as aortic stenosis and mitral regurgitation. The ability to create patient-specific models contributes significantly to predicting and preventing complications like paravalvular leakage, ensuring optimal device selection, and improving outcomes. Additionally, 3D printing extends its impact beyond valvular diseases to tricuspid regurgitation and non-valvular structural heart conditions. The comprehensive synthesis of the existing literature presented here emphasizes the promising trajectory of individualized approaches facilitated by 3D printing, promising a future where tailored interventions based on precise anatomical considerations become standard practice in cardiovascular care. [ABSTRACT FROM AUTHOR]

Double-origin right bronchial artery is a rare anatomical variation with varying presentation. Patients can be asymptomatic, but in case they present with hemoptysis, such anatomical variations may lead to clinical dilemmas. In this case report, we describe a young patient who presented with massive hemoptysis and was treated with superselective embolization. To the best of our knowledge, double-origin right bronchial artery has been rarely described in the literature. Herein, we aim to highlight the importance of understanding the anatomy and the variants of bronchial arteries. [ABSTRACT FROM AUTHOR]

Cell culture devices, such as microwells and microfluidic chips, are designed to increase the complexity of cell-based models while retaining control over culture conditions and have become indispensable platforms for biological systems modelling. From microtopography, microwells, plating devices, and microfluidic systems to larger constructs such as live imaging chamber slides, a wide variety of culture devices with different geometries have become indispensable in biology laboratories. However, while their application in biological projects is increasing exponentially, due to a combination of the techniques, equipment and tools required for their manufacture, and the expertise necessary, biological and biomedical labs tend more often to rely on already made devices. Indeed, commercially developed devices are available for a variety of applications but are often costly and, importantly, lack the potential for customisation by each individual lab. The last point is quite crucial, as often experiments in wet labs are adapted to whichever design is already available rather than designing and fabricating custom systems that perfectly fit the biological question. This combination of factors still restricts widespread application of microfabricated custom devices in most biological wet labs. Capitalising on recent advances in bioengineering and microfabrication aimed at solving these issues, and taking advantage of low-cost, high-resolution desktop resin 3D printers combined with PDMS soft lithography, we have developed an optimised a low-cost and highly reproducible microfabrication pipeline. This is thought specifically for biomedical and biological wet labs with not prior experience in the field, which will enable them to generate a wide variety of customisable devices for cell culture and tissue engineering in an easy, fast reproducible way for a fraction of the cost of conventional microfabrication or commercial alternatives. This protocol is designed specifically to be a resource for biological labs with limited expertise in those techniques and enables the manufacture of complex devices across the μm to cm scale. We provide a ready-to-go pipeline for the efficient treatment of resin-based 3D-printed constructs for PDMS curing, using a combination of polymerisation steps, washes, and surface treatments. Together with the extensive characterisation of the fabrication pipeline, we show the utilisation of this system to a variety of applications and use cases relevant to biological experiments, ranging from micro topographies for cell alignments to complex multipart hydrogel culturing systems. This methodology can be easily adopted by any wet lab, irrespective of prior expertise or resource availability and will enable the wide adoption of tailored microfabricated devices across many fields of biology. The design and fabrication of customized microwells and microfluidic chips is often challenging for biological labs due limited resources and expertise. This study describes a ready-to-go pipeline for the efficient treatment of resin-based 3D printed constructs for PDMS curing, and provides a variety of applications in biological experiments. [ABSTRACT FROM AUTHOR]

Leptospirosis is a reemerging zooanthroponosis with a worldwide distribution, though it has a higher incidence in areas with tropical climate. A characteristic finding of the disease is its wide spectrum of symptoms and organ involvement, as it can appear either with very mild flu-like manifestations or with multiorgan failure, affecting the central nervous system (CNS) with a concomitant hepatorenal dysfunction (Weil's syndrome) and significant high mortality rate. We report herein a fatal case of a 25 years old female, previously healthy, with impaired neurological status. She had high fever and severe multiorgan failure. The clinical data and the epidemiological factors were not conclusive for the diagnosis, and the first serology test from the cerebrospinal fluid (CSF) and sera samples were negative. When the repetition of the blood test showed elevated IgM antibodies, Leptospirosis was the presumptive diagnosis. Although CNS involvement is rare, the diagnosis should be considered when there is an elevated risk of exposure. The diagnostic protocol should encompass direct evidence of the bacterium and indirect measurement of antibodies. Timely detection and management are imperative to forestall complications and fatality associated with the disease. [ABSTRACT FROM AUTHOR]

Background: Although widely used, the ADVIA 120 hematology analyzer has not been previously validated for determining the differential leukocyte count in goats. Objectives: The aim of this study was to compare the differential leukocyte counts provided by the ADVIA 120 (A‐diff) and the manual method (M‐Diff) in goats. Methods: EDTA blood samples that were analyzed within 4 h of collection were used in the study. The following exclusion criteria were applied: inappropriately filled tubes or tubes containing clots, erroneous ADVIA peroxidase cytograms, and blood smears of poor quality. The A‐Diff was compared with the M‐Diff performed by two independent observers on 200 leukocytes. Results: Forty samples were included after previously excluding eight samples. The correlation between the A‐Diff and M‐Diff was very strong for eosinophils (r =.870, p <.001) and strong for lymphocytes (r =.796, p <.001) and neutrophils (r =.730, p <.001), while no significant correlation was observed for monocytes (r =.026, p =.872). The Passing–Bablok regression analyses revealed statistically significant constant errors for neutrophils (5.83%; 95% confidence interval [CI]: 0.41%, 12.18%) and eosinophils (1.89%; 95% CI: 1.17%, 2.71%). Bland–Altman analyses showed a statistically significant negative bias for lymphocytes (−5.0%) and a statistically significant positive bias for eosinophils (2.2%). The very low basophil percentages precluded a meaningful method comparison. Conclusions: The ADVIA 120 overall demonstrated good performance for the differential WBC count in goats under the conditions of this study. Therefore, it can be considered suitable for routine hematologic screening in goats. Nonetheless, it should be emphasized that any abnormal result should be confirmed with a blood smear evaluation. [ABSTRACT FROM AUTHOR]

Background: Beau's lines are transverse grooves in the nail plate that result from transient interruption of the growth of the proximal nail matrix after severe disease. The aim of this study is to systematically report all evidence on the association of Beau's lines with COVID-19 infection or vaccination against COVID-19. Methods: PubMed and Scopus databases were searched up to January 2024 for articles reporting Beau's lines associated with COVID-19 infection or vaccination for COVID-19. PROSPERO ID: CRD42024496830. Results: PubMed search identified 299 records while Scopus search identified 18 records. After screening the bibliography, nine studies including 35 cases were included in our systematic review. The studies were reported from different areas around the world. Included studies documented Beau's lines following COVID-19 vaccination (two studies) or after COVID-19 infection (seven studies). High variability was recorded in onset and resolution times among included cases, averaging 3 months and 6 months after COVID-19 infection, respectively. In the two studies reporting Beau's lines after vaccination, onset was at 7 days and 6 weeks and resolution occurred after 8 and 17 weeks, respectively. Conclusions: To the best of our knowledge, this is the first systematic review reporting the association of Beau's lines with COVID-19 infection and vaccination. Severe immune response can result in the formation of these nail disorders. Of importance, Beau's lines represent a potential indicator of prior severe COVID-19 infection or vaccination for COVID-19, as well as a sign of long COVID-19 syndrome. [ABSTRACT FROM AUTHOR]