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The Action-sentence Compatibility Effect (ACE) is a well-known demonstration of the role of motor activity in the comprehension of language. Participants are asked to make sensibility judgments on sentences by producing movements toward the body or away from the body. The ACE is the finding that movements are faster when the direction of the movement (e.g., toward) matches the direction of the action in the to-be-judged sentence (e.g., Art gave you the pen describes action toward you). We report on a pre-registered, multi-lab replication of one version of the ACE. The results show that none of the 18 labs involved in the study observed a reliable ACE, and that the meta-analytic estimate of the size of the ACE was essentially zero. [ABSTRACT FROM AUTHOR]

Aims Cardiomyocyte Ca2+ homeostasis is altered with aging via poorly-understood mechanisms. The Transient Receptor Potential Vanilloid 4 (TRPV4) ion channel is an osmotically-activated Ca2+ channel, and there is limited information on the role of TRPV4 in cardiomyocytes. Our data show that TRPV4 protein expression increases in cardiomyocytes of the aged heart. The objective of this study was to examine the role of TRPV4 in cardiomyocyte Ca2+ homeostasis following hypoosmotic stress and to assess the contribution of TRPV4 to cardiac contractility and tissue damage following ischaemia–reperfusion (I/R), a pathological condition associated with cardiomyocyte osmotic stress. Methods and results TRPV4 protein expression increased in cardiomyocytes of Aged (24–27 months) mice compared with Young (3–6 months) mice. Immunohistochemistry revealed TRPV4 localization to microtubules and the t-tubule network of cardiomyocytes of Aged mice, as well as in left ventricular myocardium of elderly patients undergoing surgical aortic valve replacement for aortic stenosis. Following hypoosmotic stress, cardiomyocytes of Aged, but not Young exhibited an increase in action-potential induced Ca2+ transients. This effect was mediated via increased sarcoplasmic reticulum Ca2+ content and facilitation of Ryanodine Receptor Ca2+ release and was prevented by TRPV4 antagonism (1 μmol/L HC067047). A similar hypoosmotic stress-induced facilitation of Ca2+ transients was observed in Young transgenic mice with inducible TRPV4 expression in cardiomyocytes. Following I/R, isolated hearts of Young mice with transgenic TRPV4 expression exhibited enhanced contractility vs. hearts of Young control mice. Similarly, hearts of Aged mice exhibited enhanced contractility vs. hearts of Aged TRPV4 knock-out (TRPV4−/−) mice. In Aged, pharmacological inhibition of TRPV4 (1 μmol/L, HC067047) prevented hypoosmotic stress-induced cardiomyocyte death and I/R-induced cardiac damage. Conclusions Our findings provide a new mechanism for hypoosmotic stress-induced cardiomyocyte Ca2+ entry and cell damage in the aged heart. These finding have potential implications in treatment of elderly populations at increased risk of myocardial infarction and I/R injury. [ABSTRACT FROM AUTHOR]

Infection of humans with the spirochete, Borrelia burgdorferi, causes Lyme borreliosis and can lead to clinical manifestations such as arthritis, carditis, and neurological conditions. Experimental infection of mice recapitulates many of these symptoms and serves as a model system for the investigation of disease pathogenesis and immunity. Innate immunity is known to drive the development of Lyme arthritis and carditis, but the mechanisms driving this response remain unclear. Innate immune cells recognize B. burgdorferi surface lipoproteins primarily via toll-like receptor (TLR)2; however, previous work has demonstrated TLR2-/- mice had exacerbated disease and increased bacterial burden. We demonstrate increased CD4 and CD8 T cell infiltrates in B. burgdorferi-infected joints and hearts of C3H TLR2-/- mice. In vivo depletion of either CD4 or CD8 T cells reduced Borrelia-induced joint swelling and lowered tissue spirochete burden, whereas depletion of CD8 T cells alone reduced disease severity scores. Exacerbation of Lyme arthritis correlated with increased production of CXCL9 by synoviocytes, and this was reduced with CD8 T cell depletion. These results demonstrate T cells can exacerbate Lyme disease pathogenesis and prolong disease resolution possibly through dysregulation of inflammatory responses and inhibition of bacterial clearance. [ABSTRACT FROM AUTHOR]

Recent studies attribute top-down control as the primary determinant of the speed with which attention can be disengaged from an object, and disengagement has received increased interest as a means to distinguish between top-down and bottom-up accounts of attention capture. We present the results of three experiments exploring the breadth of the representations that delay attentional disengagement based on top-down goals. Experiments 1 and 2 examined whether objects similar to an observers' search target, but differing in luminance and hue, delayed the reallocation of attention in a search paradigm designed to isolate disengagement time. Experiment 3 explored whether the representations that delay disengagement are based on absolute similarity with the search target, or are tuned based on target/nontarget relationships. These three studies confirmed the role of top-down goals in automatically contributing to dwell times and revealed that the representations that underlie disengagement effects are broad (automatically delaying disengagement for items similar, but not identical to, the search target). In some cases, attention sets appeared to be graded in nature, but in others target–distractor relationships influenced the degree to which an irrelevant item held attention. Implications for theories of attention capture and potential functional significance of these automatic effects are discussed. [ABSTRACT FROM PUBLISHER]

Power-the ability to influence the outcomes of other people-is a key variable that regulates a wide range of human social interactions. Although previous research has demonstrated that power leads people to become approach-oriented, most studies have focused on how this orientation manifests itself in conscious, higher-order aspects of social behavior. The current study presents evidence that priming the concept of power has a direct influence on low level processes within the motor system. Participants performed a task in which they responded to auditory cues by moving their hand either toward the immediate environment (approach) or away from the environment (avoidance). Priming the concept of power facilitated the initiation of approach responses and, to a lesser degree, interfered with initiation of avoidance responses. This study supplements theories of power and approach, and fits with recent work suggesting fundamental links between cognitive processes and motor behavior. [ABSTRACT FROM AUTHOR]

Kaschak and Saffran (2006) reported that the presence of an idiomatic syntactic construction (i.e., a construction whose structure violates the rules that govern the structure of the other sentences in the language) in training had a positive effect on participants' ability to acquire the phrase structure of an artificial language, but only when the idiomatic construction was given a distinctive prosodic feature. The present study extends this finding by exploring whether the distinctive meanings that accompany idiomatic constructions also influence their effect on language learning. We report two experiments in which Kaschak and Saffran's paradigm is replicated with one important change: whereas the previous study provided participants with only auditory training, the current studies added a visual reference world (i.e., semantics) to the training. Our experiments failed to reproduce the beneficial effects of the idiomatic construction on the acquisition of the phrase structure of the language. This result places important limitations on the role that idiomatic constructions play in language acquisition. [ABSTRACT FROM AUTHOR]

The brownDominant (bwD) allele contains a large insertion of heterochromatin, which causes the locus to aberrantly associate with heterochromatin in interphase nuclei and silences the wild-type allele in heterozygotes. Transgenes placed near the bw+ locus, in trans to bwD, can also be silenced. The strength of silencing (called trans inactivation) varies with the regulatory sequences of the transgene and its distance away from the bwD insertion site in trans. In this study, we examine endogenous sequences in cis that influence susceptibility of a reporter gene to trans inactivation. Flanking deletions were induced in two parental lines containing P-element transgenes showing trans inactivation of the mini-white reporter. These new lines, which have mini-white under the influence of different endogenous sequence elements, now show varied ability to be silenced by bwD. Determination of the deleted regions and the levels of mini-white expression and trans inactivation has allowed us to explore the correlation between cis sequence elements and susceptibility to trans inactivation and to identify a 301-bp sequence that acts as an enhancer of trans inactivation. Intriguingly, this region encompasses the upstream regions of two divergently transcribed genes and contains a sequence motif that may bind BEAF, a protein involved in delimiting chromatin boundaries. [ABSTRACT FROM AUTHOR]