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Effects of Cr alloying on phase stability, magnetism, and electronic structures in both body‐centered cubic (bcc) and face‐centered cubic (fcc) phases and on the transformation from fcc to bcc are studied by first‐principles calculations. Results show that the doped Cr atoms in fcc and bcc phases choose distinct occupation models. This phenomenon can be understood from the amount of electron density of states close to Fermi energy. For magnetism, Cr tends to be antiferromagnetically coupled with the surrounding Fe in the studied phases. The magnetic moment of Fe is greater than that of Cr in bcc, but the order is reversed in fcc. The moment of Fe is dictated by the distance between it and the doped Cr in bcc, whereas it is dominated by spatial orientation with Cr in fcc. For phase stability, it is found that the alloying of Cr prefers destabilizing bcc while tends to stabilize fcc, leading to a strong inhibition of phase transition from fcc to bcc. Notably, the role in the fcc phase is more prominent than that in bcc, which can be associated with the antiferromagnetism between Fe and Cr in fcc. [ABSTRACT FROM AUTHOR]

Seafloor topography is essential for oceanic fluid dynamics from many perspectives, and it is believed to enhance energy dissipation in oceanic flows. This study numerically examines the impact of small-scale topography on the dynamics of quasi-geostrophic barotropic flows and finds three regimes and two critical values of topographic magnitude. In the first regime, where the topographic magnitude is below the first critical value, small-amplitude topography extends the range of upscale energy flux and enhances condensation, contradicting the common understanding of topographic damping. In the second regime, where the topography magnitude lies between the two critical values, energy simultaneously transfers to both large and small scales, and topography induces extra dissipation at the topographic scale. A second critical topography magnitude exists, corresponding to a second-order phase transition. When the topography magnitude exceeds the second critical value, energy only transfers downscale. The discovery of counterintuitive topography-enhanced energy flux and its critical phenomenon brings new challenges to topography parameterization in ocean models. [ABSTRACT FROM AUTHOR]

The presence of atherosclerotic plaque vessels is a critical factor in plaque destabilization. This may be attributable to the leaky phenotype of these microvessels, although direct proof for this notion is lacking. In this study, we investigated molecular and cellular patterns of stable and hemorrhaged human plaque to identify novel drivers of intraplaque vessel dysfunction. From transcriptome data of a human atherosclerotic lesion cohort, we reconstructed a co-expression network, identifying a gene module strongly and selectively correlated with both plaque microvascular density and inflammation. Spectrin Beta Non-Erythrocytic 1 (sptbn1) was identified as one of the central hubs of this module (along with zeb1 and dock1) and was selected for further study based on its predominant endothelial expression. Silencing of sptbn1 enhanced leukocyte transmigration and vascular permeability in vitro, characterized by an increased number of focal adhesions and reduced junctional VE-cadherin. In vivo, sptbn1 knockdown in zebrafish impaired the development of the caudal vein plexus. Mechanistically, increased substrate stiffness was associated with sptbn1 downregulation in endothelial cells in vitro and in human vessels. Plaque SPTBN1 mRNA and protein expression were found to correlate with an enhanced presence of intraplaque hemorrhage and future cardiovascular disease (CVD) events during follow-up. In conclusion, we identify SPTBN1 as a central hub gene in a gene program correlating with plaque vascularisation. SPTBN1 was regulated by substrate stiffness in vitro while silencing blocked vascular development in vivo, and compromised barrier function in vitro. Together, SPTBN1 is identified as a new potential regulator of the leaky phenotype of atherosclerotic plaque microvessels. [ABSTRACT FROM AUTHOR]

Utilizing firms in the S&P 500, we study whether greater transparency in the reporting of other comprehensive income (OCI) items, as mandated by ASU 2011-05, resulted in a reduction in information asymmetry, a change in the value relevance of this information, or a change in hedging practice. Our results show that while transparent reporting reduced information asymmetry, firms that engage in cash flow hedging do have greater information asymmetry than their counterparts that do not hedge. We find evidence that investors penalize firm value for greater volatility of OCI relative to net income volatility when reported transparently. When permitted, managers were able to mitigate the negative impact by reporting OCI only in the Statement of Shareholders' Equity. We conclude that managers' concerns regarding potential confusion surrounding OCI volatility following more prominent reporting led to changes in hedging behavior. After transparent reporting, we find a reduced likelihood of foreign currency cash flow (FXCF) hedges and a reduced level of FXCF hedging among firms experiencing the greatest volatility of unrealized hedging gains and losses. [ABSTRACT FROM AUTHOR]

Methicillin-resistant Staphylococcus aureus (MRSA) is a serious threat to patients with nosocomial infections, and infection is strongly associated with biofilm formation. Gallic acid (GA) is a natural bioactive compound found in traditional Chinese medicines that exerts potent antimicrobial activity. However, the anti-MRSA biofilm efficacy of GA remained to be determined. This study investigated the antimicrobial activities of GA against MRSA and the mechanisms involved. The results revealed the significant antibacterial and antibiofilm activities of GA. The minimal inhibitory concentration of GA against MRSA was 32 μg/mL and a growth curve assay confirmed the significant inhibitory effect of GA on planktonic MRSA. Crystal violet and XTT assays showed that 8 µg/mL GA effectively inhibited the formation of new biofilms and disrupted existing biofilms by reducing both biofilm biomass and metabolic activities. Alkaline phosphatase and β-galactosidase leakage assays and live/dead staining provided evidence that GA disrupted the integrity of bacterial cell walls and membranes within the biofilm. Scanning electron microscopy observations showed that GA significantly inhibited bacterial adhesion and aggregation, affecting the overall structure of the biofilm. Bacterial adhesion, polysaccharide intercellular adhesion (PIA) production and real-time quantitative PCR assay confirmed that GA inhibited bacterial adhesion, PIA synthesis, and the expression of icaAD and sarA. These results suggested that GA inhibited biofilm formation by inhibiting the expression of sarA, then downregulating the expression of icaA and icaD, thereby reducing the synthesis of PIA to attenuate the adhesion capacity of MRSA. GA is therefore a promising candidate for development as a pharmaceutical agent for the prevention and treatment of bacterial infections caused by MRSA. [ABSTRACT FROM AUTHOR]

Background: Metabolism is increasingly recognized as a key regulator of the function and phenotype of the primary cellular constituents of the atherosclerotic vascular wall, including endothelial cells, smooth muscle cells, and inflammatory cells. However, a comprehensive analysis of metabolic changes associated with the transition of plaque from a stable to a hemorrhaged phenotype is lacking. Methods: In this study, we integrated two large mRNA expression and protein abundance datasets (BIKE, n = 126; MaasHPS, n = 43) from human atherosclerotic carotid artery plaque to reconstruct a genome-scale metabolic network (GEM). Next, the GEM findings were linked to metabolomics data from MaasHPS, providing a comprehensive overview of metabolic changes in human plaque. Results: Our study identified significant changes in lipid, cholesterol, and inositol metabolism, along with altered lysosomal lytic activity and increased inflammatory activity, in unstable plaques with intraplaque hemorrhage (IPH+) compared to non-hemorrhaged (IPH−) plaques. Moreover, topological analysis of this network model revealed that the conversion of glutamine to glutamate and their flux between the cytoplasm and mitochondria were notably compromised in hemorrhaged plaques, with a significant reduction in overall glutamate levels in IPH+ plaques. Additionally, reduced glutamate availability was associated with an increased presence of macrophages and a pro-inflammatory phenotype in IPH+ plaques, suggesting an inflammation-prone microenvironment. Conclusions: This study is the first to establish a robust and comprehensive GEM for atherosclerotic plaque, providing a valuable resource for understanding plaque metabolism. The utility of this GEM was illustrated by its ability to reliably predict dysregulation in the cholesterol hydroxylation, inositol metabolism, and the glutamine/glutamate pathway in rupture-prone hemorrhaged plaques, a finding that may pave the way to new diagnostic or therapeutic measures. [ABSTRACT FROM AUTHOR]

Facing the dual pressures of the exacerbation of global climate change and the deterioration of the domestic environment caused by pollution, China has clearly adopted environmental regulatory measures to improve the climate environment. One measure is the carbon emissions trading scheme (CETS), which serves as a notable example of the country's efforts to improve the climate environment. We gathered panel data from 285 prefecture‐level cities in China from 2005 to 2018 and used the Difference‐in‐Differences (DID) model to empirically examine the synergistic effects of the CETS on carbon emissions and air pollution. The results indicate that CETS have been effective in reducing urban carbon emissions by approximately 9.8%. Additionally, the schemes have caused a simultaneous reduction in particulate matter (PM)2.5 emissions by 11.7% and sulfur dioxide (SO2) emissions by approximately 9.7%, mitigating urban air pollution in China. It demonstrates that the scheme has significant synergistic effects on carbon emissions and air pollution. To achieve synergistic effects of CETS, effective measures include reducing energy intensity and upgrading the industrial structure. The implementation of CETS had heterogeneity in different conditions, and the synergistic effect of the scheme is more significant in eastern regions, large cities, and the final industrial stage. Our findings offer innovative solutions for the integrated management of carbon emissions and air pollution and provide valuable insights for policymakers to enhance China's CETS. Integr Environ Assess Manag 2024;20:1112–1124. © 2023 SETAC Key Points: The carbon emissions trading scheme (CETS) led not only to the significant reduction by approximately 9.8% in carbon dioxide (CO2) emissions, but also effectively mitigated sulfur dioxide emissions by approximately 9.7% and particulate matter (PM)2.5 emissions by approximately 11.7%.This policy highlights the synergistic impact of reducing carbon emissions and air pollution.The effective emission reduction measures of CETS are focused on reducing energy intensity and upgrading industrial structure; the proper implementation of these measures can achieve significant synergistic emission reduction.Based on the heterogeneous analysis of the pilot scheme, the synergistic effects of the CETS are particularly significant in the eastern regions, large cities, and the final industrial stages. [ABSTRACT FROM AUTHOR]

Key Clinical Message: In previous reports, hypothyroidism, hypopituitrism, and hypogonadism were common endocrine causes of SCFE, but this is the first time that congenital adrenal hyperplasia has been observed. As such, patients who have undergone long‐term endocrine treatment for congenital adrenal hyperplasia could potentially be subjected to a higher risk for SCFE. [ABSTRACT FROM AUTHOR]

Gastric cancer is the fifth most common disease in the world and the fourth most common cause of death. It is diagnosed through esophagogastroduodenoscopy with biopsy; however, there are limitations in finding lesions in the early stages. Recently, research has been actively conducted to use liquid biopsy to diagnose various cancers, including gastric cancer. Various substances derived from cancer are reflected in the blood. By analyzing these substances, it was expected that not only the presence or absence of cancer but also the type of cancer can be diagnosed. However, the amount of these substances is extremely small, and even these have various variables depending on the characteristics of the individual or the characteristics of the cancer. To overcome these, we collected methylated DNA fragments using MeDIP and compared them with normal plasma to characterize gastric cancer tissue or patients' plasma. We attempted to diagnose gastric cancer using the characteristics of cancer reflected in the blood through the cancer tissue and patients' plasma. As a result, we confirmed that the consistency of common methylated fragments between tissue and plasma was approximately 41.2% and we found the possibility of diagnosing and characterizing cancer using the characteristics of the fragments through SFR and 5′end-motif analysis. [ABSTRACT FROM AUTHOR]

Introduction: Special needs dentistry (SND) is an emerging dental specialty, with ongoing developments in education and clinical practice focused towards the tailored management of individuals with special needs (SN). Patients with SN have a higher prevalence of oral diseases and unmet dental needs compared to the general population. Although inadequate training and experience in managing patients with SN has been highlighted as a significant barrier to accessing care, there is limited data about the extent of SND teaching at the entry‐to‐practice or higher levels. Methods: This work is the first to map SND curricula globally, across 180 countries and 1265 dental schools. Results: Although 74.62% of dental schools were found in developing economies, the distribution of programs that reported SND in their courses was highly skewed towards developed countries. In terms of advanced degrees, beyond basic entry‐to‐practice training, the USA delivered 60% of the SND programs, followed by Canada (15.56%), UK (13.33%), and Australia (8.89%). The term SND appeared in 33.95% of entry‐to‐practice level program curricula and was less commonly used in transitioning economies. Only 112 SND‐specialized practitioners enter the workforce globally each year from developed economies, and all but three advanced degrees are found in G7 countries. Conclusion: By exploring the impact of economic status on its distribution, this paper highlighted the lack of SND representation in dental curricula, especially amongst programs in transitioning or developing economies. Education of both general dentists and specialists is critical as a collaborative effort is needed to manage the growing population of patients with SN. [ABSTRACT FROM AUTHOR]

In this paper, we systematically investigate the general dark matter-electron interactions within the framework of effective field theories (EFTs). We consider both the non-relativistic (NR) EFT and the relativistic EFT descriptions of the interactions with the spin of dark matter (DM) up to one, i.e., the scalar (ϕ), fermion (χ), and vector (X) DM scenarios. We first collect the leading-order NR EFT operators describing the DM-electron interactions, and construct especially the NR operators for the vector DM case. Next, we consider all possible leading-order relativistic EFT operators including those with a photon field and perform the NR reduction to match them onto the NR EFT. Then we rederive the DM-bound-electron scattering rate within the NR EFT framework and find that the matrix element squared, which is the key input that encodes the DM and atomic information, can be compactly decomposed into three terms. Each term is a product of a DM response function (a0,1,2), which is essentially a factor of Wilson coefficients squared, and its corresponding generalized atomic response function W ~ 0 , 1 , 2 . Lastly, we employ the electron recoil data from the DM direct detection experiments (including XENON10, XENON1T, and PandaX-4T) to constrain all the non-relativistic and relativistic operators in all three DM scenarios. We set strong bounds on the DM-electron interactions in the sub-GeV region. Particularly, we find that the latest PandaX-4T S2-only data provide stringent constraints on dark matter with a mass greater than approximately 20 MeV, surpassing those from the previous XENON10 and XENON1T experiments. [ABSTRACT FROM AUTHOR]

Walking can have a positive impact on cognitive function in adolescents. This study aimed to compare the effects of walking with sneakers and barefoot on cognitive ability in adolescents. Fifty-nine adolescent male students were included in the study and assigned to the control (n = 20), sneaker (n = 19), and barefoot (n = 20) groups. The barefoot and sneakers group performed a 40-min walking exercise four times a week for 12 weeks during the morning physical activity time, while the control group performed self-study. Electroencephalogram (EEG) and brain activity variables were measured before and after the exercise program. The results showed that after 12 weeks, the barefoot group had a significant decrease in Gamma and H-beta waves and a significant increase in sensorimotor rhythm (SMR) and Alpha waves. Conversely, the control group showed a significant decrease in SMR waves and increase in Theta waves. The sneaker group showed a significant decrease in SMR waves alone. In an eyes-open resting state, the barefoot group showed a significant increase in H-beta, M-beta, SMR, and Alpha waves. The barefoot group also had a significant increase in cognitive speed and concentration and a significant decrease in brain stress. Taken together, barefoot walking can effectively enhance cognitive ability in adolescents, as demonstrated by the significant variation in EEG activity. This research highlights the potential benefits of barefoot walking as a simple and effective form of exercise for enhancing cognitive function in adolescents. [ABSTRACT FROM AUTHOR]

Macrophage activation syndrome (MAS) is potentially fatal; so, early diagnosis and timely treatment are essential. However, detecting MAS is sometimes challenging because its principal features can be observed in other pediatric diseases that cause severe inflammation. Cytokine storm due to immune dysregulation represents the clinical and laboratory features of MAS that are included in the diagnostic criteria. Most cases of MAS occur as an underlying condition worsens and progresses. Therefore, a patient with autoimmune or autoinflammatory disease who shows unexplained clinical deterioration despite appropriate management should be considered at high risk for MAS (i.e., occult MAS). The basic principles of treatment are control of triggering factors, supportive care, and relief of hyperinflammation. Systemic steroids and cyclosporine A are frequently used as a first-line treatment. For the treatment of refractory MAS, cytokine-specific biologic agents such as anakinra have recently become preferred over traditional immunosuppressive agents such as etoposide. MAS might be underrecognized in pediatric patients with infectious and inflammatory diseases due to its diverse clinical presentations. Clinical suspicion of MAS is of the utmost importance for early recognition of the disease. [ABSTRACT FROM AUTHOR]

Pyruvate kinase M2 (PKM2), a rate limiting enzyme in glycolysis, is a cellular regulator that has received extensive attention and regards as a metabolic regulator of cellular metabolism and energy. Kidney is a highly metabolically active organ, and glycolysis is the important energy resource for kidney. The accumulated evidences indicates that the enzymatic activity of PKM2 is disturbed in kidney disease progression and treatment, especially diabetic kidney disease and acute kidney injury. Modulating PKM2 post-translational modification determines its enzymatic activity and nuclear translocation that serves as an important interventional approach to regulate PKM2. Emerging evidences show that PKM2 and its post-translational modification participate in kidney disease progression and treatment through modulating metabolism regulation, podocyte injury, fibroblast activation and proliferation, macrophage polarization, and T cell regulation. Interestingly, PKM2 activators (TEPP-46, DASA-58, mitapivat, and TP1454) and PKM2 inhibitors (shikonin, alkannin, compound 3k and compound 3h) have exhibited potential therapeutic property in kidney disease, which indicates the pleiotropic effects of PKM2 in kidney. In the future, the deep investigation of PKM2 pleiotropic effects in kidney is urgently needed to determine the therapeutic effect of PKM2 activator/inhibitor to benefit patients. The information in this review highlights that PKM2 functions as a potential biomarker and therapeutic target for kidney diseases. [ABSTRACT FROM AUTHOR]

CD8 + T cells exert a critical role in eliminating cancers and chronic infections, and can provide long-term protective immunity. However, under the exposure of persistent antigen, CD8 + T cells can differentiate into terminally exhausted CD8 + T cells and lose the ability of immune surveillance and disease clearance. New insights into the molecular mechanisms of T-cell exhaustion suggest that it is a potential way to improve the efficacy of immunotherapy by restoring the function of exhausted CD8 + T cells. Transforming growth factor-β (TGF-β) is an important executor of immune homeostasis and tolerance, inhibiting the expansion and function of many components of the immune system. Recent studies have shown that TGF-β is one of the drivers for the development of exhausted CD8 + T cells. In this review, we summarized the role and mechanisms of TGF-β in the formation of exhausted CD8 + T cells and discussed ways to target those to ultimately enhance the efficacy of immunotherapy. [ABSTRACT FROM AUTHOR]

The formations in the early Paleozoic Joseon Supergroup (mainly carbonates with subordinate siliciclastics) within the Taebaeksan Basin in the Danyang area, South Korea, show an apparent right-side-up homoclinal stratigraphy without repetition or omission of any formation, and it was therefore thought that the NW-dipping formation boundaries are primary depositional contacts. Our detailed examination revealed, however, that the formation boundaries are reverse-slip shear zones parallel to the second-generation foliation (S2) crenulating transposed S0//S1 foliation and that WNW-dipping bedding planes (S0) are only locally preserved. The most penetrative regional planar structure in the area is the NNW-dipping first-generation foliation (S1) that is defined by compositional layering and is parallel to the axial planes of isoclinal F1 folds. Isoclinal to close F1 folds occur on both the mesoscopic and macroscopic scale, whereas tight to close F2 folds occur locally only on the mesoscopic scale. D1 deformation presumably involved a NNW-SSE horizontal contraction and resulted in the buckle folding and transposition of S0. D2 deformation involved a NW-SE contraction, probably at a high angle to the S0//S1 transposed layers, and produced passive shear folding of S0//S1 with S2 crenulation cleavages as discrete shear surfaces. Shearing deformation was strongly localized along some S2 foliation planes, resulting in reverse shear zones that represent the current 'pseudo'-formation boundaries. Based on zircons U-Pb ages, we suggest that the Okdong Fault was initiated from an unconformable boundary between the Precambrian basement gneiss and overlying sediments of the Paleozoic Taebaeksan Basin, probably during the Middle Jurassic. [ABSTRACT FROM AUTHOR]

The 2016 magnitude (MW) 5.5 Gyeongju earthquake, which occurred in Korea near assumed epicenters of several substantial historical earthquakes and Quaternary fault segments, underscores the importance of seismic hazard assessment in the region. However, uncertainties about potential seismic sources make evaluating the potential for a moderate-to-large earthquake challenging. Microearthquake monitoring through a dense seismic network can provide crucial insights into the regional seismic characteristics. An extensive temporary seismic array known as the Gyeongju Hi-density Broadband Seismic Network (GHBSN) was established to investigate microearthquake activity in the southeastern Korean Peninsula. This included the zone of aftershocks from the 2016 Gyeongju earthquake sequence. The GHBSN comprises 200 broadband stations located at approximately 4.5 km intervals in an area of approximately 60 × 60 km2 around the epicenter of the mainshock. A total of 4,773 events were detected from November 2017 to December 2021, including 3,935 events within the GHBSN. The detected events were categorized into five seismic regions excluding quarry blasting sites, that is, the 2016 Gyeongju earthquake region, eastern part of the Ulsan Fault, 2017 Pohang earthquake region, eastern offshore Gyeongju, and western part of the Miryang Fault. A local magnitude scale was developed for the southeastern Korean Peninsula using events detected through the GHBSN. This reflects the distance attenuation and site conditions of the GHBSN stations for earthquakes. An event catalog was created using two automatic detection methods based on the measurement of the energy ratio. This provided high-resolution hypocenter parameters at a completeness magnitude (MC) of 0.0 despite the seismic environment of the network being exposed to high cultural noise. The Gutenberg-Richter b-value was estimated as 0.82 ± 0.02 for all events and 1.01 ± 0.02 for those inside GHBSN. This implies that the seismicity reflects a representative intraplate seismic environment. Testing the obtainability of the focal mechanism solutions showed that the GHBSN outperformed the regional network. Depending on the relationship between the magnitude and frequency of earthquakes, a relatively large number of small earthquakes can provide detailed information on the geometric properties of the causative faults and the state of the acting stress. High-precision microearthquake observation and analysis through GHBSN could provide an unprecedented opportunity with seismic datasets to understand the seismogenesis of the southeastern Korean Peninsula, including the zone of aftershocks of the 2016 Gyeongju earthquake. [ABSTRACT FROM AUTHOR]

In addition to cellular damage, ischemia-reperfusion (IR) injury induces substantial damage to the mitochondria and endoplasmic reticulum. In this study, we sought to determine whether impaired mitochondrial function owing to IR could be restored by transplanting mitochondria into the heart under ex vivo IR states. Additionally, we aimed to provide preliminary results to inform therapeutic options for ischemic heart disease (IHD). Healthy mitochondria isolated from autologous gluteus maximus muscle were transplanted into the hearts of Sprague-Dawley rats damaged by IR using the Langendorff system, and the heart rate and oxygen consumption capacity of the mitochondria were measured to confirm whether heart function was restored. In addition, relative expression levels were measured to identify the genes related to IR injury. Mitochondrial oxygen consumption capacity was found to be lower in the IR group than in the group that underwent mitochondrial transplantation after IR injury (p < 0.05), and the control group showed a tendency toward increased oxygen consumption capacity compared with the IR group. Among the genes related to fatty acid metabolism, Cpt1b (p < 0.05) and Fads1 (p < 0.01) showed significant expression in the following order: IR group, IR + transplantation group, and control group. These results suggest that mitochondrial transplantation protects the heart from IR damage and may be feasible as a therapeutic option for IHD. [ABSTRACT FROM AUTHOR]

Introduction: Aryl hydrocarbon receptor (AhR) is a transcription factor that performs various functions upon ligand activation. Several studies have explored the role of AhR expression in tumor progression and immune surveillance. Nevertheless, investigations on the distribution of AhR expression, specifically in cancer or immune cells in the tumor microenvironment (TME), remain limited. Examining the AhR expression and distribution in the TME is crucial for gaining insights into the mechanism of action of AhR-targeting anticancer agents and their potential as biomarkers. Methods: Here, we used multiplexed immunohistochemistry (mIHC) and image cytometry to investigate the AhR expression and distribution in 513 patient samples, of which 292 are patients with one of five solid cancer types. Additionally, we analyzed the nuclear and cytosolic distribution of AhR expression. Results: Our findings reveal that AhR expression was primarily localized in cancer cells, followed by stromal T cells and macrophages. Furthermore, we observed a positive correlation between the nuclear and cytosolic expression of AhR, indicating that the expression of AhR as a biomarker is independent of its localization. Interestingly, the expression patterns of AhR were categorized into three clusters based on the cancer type, with high AhR expression levels being found in regulatory T cells (Tregs) in non-small cell lung cancer (NSCLC). Discussion: These findings are anticipated to serve as pivotal evidence for the design of clinical trials and the analysis of the anticancer mechanisms of AhRtargeting therapies. [ABSTRACT FROM AUTHOR]

The deposition and intercalation of metal atoms can induce superconductivity in monolayer and bilayer graphenes. For example, it has been experimentally proved that Li-deposited graphene is a superconductor with critical temperature Tc of 5.9 K, Ca-intercalated bilayer graphene C6CaC6 and K-intercalated epitaxial bilayer graphene C8KC8 are superconductors with Tc of 2–4 K and 3.6 K, respectively. However, the Tc of them are relatively low. To obtain higher Tc in graphene-based superconductors, here we predict a new Ca-intercalated bilayer graphene C2CaC2, which shows higher Ca concentration than the C6CaC6. It is proved to be thermodynamically and dynamically stable. The electronic structure, electron–phonon coupling (EPC) and superconductivity of C2CaC2 are investigated based on first-principles calculations. The EPC of C2CaC2 mainly comes from the coupling between the electrons of C-pz orbital and the high- and low-frequency vibration modes of C atoms. The calculated EPC constant λ of C2CaC2 is 0.75, and the superconducting Tc is 18.9 K, which is much higher than other metal-intercalated bilayer graphenes. By further applying −4% biaxial compressive strain to C2CaC2, the Tc can be boosted to 26.6 K. Thus, the predicted C2CaC2 provides a new platform for realizing superconductivity with the highest Tc in bilayer graphenes. [ABSTRACT FROM AUTHOR]

The development of a pragmatic biosensor for diagnosing acute myocardial infarction (AMI) is of paramount significance. However, the detection of cardiac Troponin I (cTnI) presents formidable challenges owing to the intricate composition and scant concentration of cTnI in clinical samples. Therefore, we propose an innovative approach employing an aptamer biosensor predicated on MXene/multi-wall carbon nanotubes (MWCNTs) to discern cTnI. By integrating the MXene/MWCNTs nanomaterial, our aptasensor manifests remarkable amplification effects, thereby enabling an extensive detection range spanning from 10 to 106 pg/mL, while attaining an unparalleled detection sensitivity with an exceptionally low limit of 6.21 pg/mL. Furthermore, our aptasensor exhibits favourable selectivity, reproducibility, and stability. Moreover, our aptasensor showcases a robust clinical correlation equation of y = 1.202x + 0.0147, accompanied by an R2 value of 0.9797. A notable p-value discrepancy is observed between the AMI and healthy control groups. Remarkably, the area under the curve (AUC) achieved amounts to 0.981, with a sensitivity of 0.938 and specificity of 0.923. These outstanding attributes, coupled with its exceptional clinical performance, position our aptasensor as the optimal choice for the practical monitoring of acute myocardial infarction (AMI) disease. [ABSTRACT FROM AUTHOR]

We study the statistically steady states of the forced dissipative three-dimensional homogeneous isotropic turbulence at scales larger than the forcing scale in real separation space. The probability density functions (p.d.f.s) of longitudinal velocity difference at large separations are close to, but deviate from, Gaussian, measured by their non-zero odd parts. The analytical expressions of the third-order longitudinal structure functions derived from the Kármán–Howarth–Monin equation prove that the odd-part p.d.f.s of velocity differences at large separations are small but non-zero. Specifically, when the forcing effect in the displacement space decays exponentially as the displacement tends to infinity, the odd-order longitudinal structure functions have a power-law decay with an exponent of $-$ 2, implying a significant coupling between large and small scales. Under the assumption that forcing controls the large-scale dynamics, we propose a conjugate regime to Kolmogorov's inertial range, independent of the forcing scale, to capture the odd parts of p.d.f.s. Thus, dynamics of large scales departs from the absolute equilibrium, and we can partially recover small-scale information without explicitly resolving small-scale dynamics. The departure from the statistical equilibrium is quantified and found to be viscosity-independent. Even though this departure is small, it is significant and should be considered when studying the large scales of the forced three-dimensional homogeneous isotropic turbulence. [ABSTRACT FROM AUTHOR]

Immunomodulatory peptides, while exhibiting potential antimicrobial, antifungal, and/or antiviral properties, can play a role in stimulating or suppressing the immune system, especially in pathological conditions like breast cancer (BC). Thus, deregulation of these peptides may serve as an immunotherapeutic strategy to enhance the immune response. In this meta-analysis, we utilized single-cell RNA sequencing data and known therapeutic peptides to investigate the deregulation of these peptides in malignant versus normal human breast epithelial cells. We corroborated our findings at the chromatin level using ATAC-seq. Additionally, we assessed the protein levels in various BC cell lines. Moreover, our in-house drug repositioning approach was employed to identify potential drugs that could positively impact the relapse-free survival of BC patients. Considering significantly deregulated therapeutic peptides and their role in BC pathology, our approach aims to downregulate B2M and SLPI, while upregulating PIGR, DEFB1, LTF, CLU, S100A7, and SCGB2A1 in BC epithelial cells through our drug repositioning pipeline. Leveraging the LINCS L1000 database, we propose BRD-A06641369 for B2M downregulation and ST-4070043 and BRD-K97926541 for SLPI downregulation without negatively affecting the MHC complex as a significantly correlated pathway with these two genes. Furthermore, we have compiled a comprehensive list of drugs for the upregulation of other selected immunomodulatory peptides. Employing an immunotherapeutic approach by integrating our drug repositioning pipeline with single-cell analysis, we proposed potential drugs and drug targets to fortify the immune system against BC. [ABSTRACT FROM AUTHOR]

Urban breeze is produced by the temperature difference between urban and surrounding rural areas, and its interactions with other atmospheric phenomena are of great interest. In this study, the impacts of background wind on the interactions between urban breeze circulation (UBC) and convective cells are numerically investigated. For this, a large number of idealized ensemble simulations are performed using the Weather Research and Forecasting‐large‐eddy simulation model, where the initial background wind speed varies from 0 to 5 m·s−1 in 0.5 m·s−1 intervals. As the background wind speed increases, the location of the strongest updraft moves from the urban center to the downwind boundary of the urban area. This is associated with different interactions between the UBC and convective cells under different background wind speeds. For no background wind, the UBC is relatively strong and its center is located at the urban center, producing a strong updraft there. Convective cells are advected toward the UBC center and merge with the strong updraft, further intensifying it. For weak background wind, the UBC is relatively weak and its center is located near the downwind urban boundary. Convective cells passing the urban area merge with each other and intensify, and after they pass the downwind urban boundary, the strong vertical wind shear in the right part of the UBC tilts and weakens them. For strong background wind, the UBC is very weak and convective cells are advected mainly by the strong background wind. These changes in the characteristics of convective cells in and around the urban area with background wind speed may lead to the changes in the preferred location of cloud initiation and cloud development. [ABSTRACT FROM AUTHOR]

White matter hyperintensity (WMH) lesions on brain MRI images are surrogate markers of cerebral small vessel disease. Longitudinal studies examining the association between diabetes and WMH progression have yielded mixed results. Thus, in this study, we investigated the association between HbA1c, a biomarker for the presence and severity of hyperglycemia, and longitudinal WMH change after adjusting for known risk factors for WMH progression. We recruited 64 participants from South Korean memory clinics to undergo brain MRI at the baseline and a 2-year follow-up. We found the following. First, higher HbA1c was associated with greater global WMH volume (WMHV) changes after adjusting for known risk factors (b = 7.7 × 1024; P = 0.025). Second, the association between baseline WMHV and WMHV progression was only significant at diabetic levels of HbA1c (P < 0.05, when HbA1c >6.51%), and non-apolipoprotein E (APOE) e4 carriers had a stronger association between HbA1c and WMHV progression (b =22.59 × 1023; P = 0.004). Third, associations of WMHV progression with HbA1c were particularly apparent for deep WMHV change (b = 7.17 × 10-4; P < 0.01) compared with periventricular WMHV change and, for frontal (b = 5.00 × 10-4; P < 0.001) and parietal (b = 1.53 × 10-4; P < 0.05) lobes, WMHV change compared with occipital and temporal WMHV change. In conclusion, higher HbA1c levels were associated with greater 2-year WMHV progression, especially in non-APOE e4 participants or those with diabetic levels of HbA1c. These findings demonstrate that diabetes may potentially exacerbate cerebrovascular and whitematter disease. [ABSTRACT FROM AUTHOR]

Purpose: Pertussis bacteria have many pathogenic and virulent antigens and severe adverse reactions have occurred when using inactivated whole-cell pertussis vaccines. Therefore, inactivated acellular pertussis (aP) vaccines and genetically detoxified recombinant pertussis (rP) vaccines are being developed. The aim of this study was to assess the safety profile of a novel rP vaccine under development in comparison to commercial diphtheria-tetanusacellular pertussis (DTaP) vaccines. Materials and Methods: The two positive control DTaP vaccines (two- and tri-components aP vaccines) and two experimental recombinant DTaP (rDTaP) vaccine (two- and tri-components aP vaccines adsorbed to either aluminum hydroxide or purified oat beta-glucan) were used. Temperature histamine sensitization test (HIST), indirect Chinese hamster ovary (CHO) cell cluster assay, mouse-weight-gain (MWG) test, leukocytosis promoting (LP) test, and intramuscular inflammatory cytokine assay of the injection site performed for safety assessments. Results: HIST results showed absence of residual pertussis toxin (PTx) in both control and experimental DTaP vaccine groups, whereas in groups immunized with tri-components vaccines, the experimental tri-components rDTaP absorbed to alum showed an ultra-small amount of 0.0066 IU/mL. CHO cell clustering was observed from 4 IU/mL in all groups. LP tests showed that neutrophils and lymphocytes were in the normal range in all groups immunized with the two components vaccine. However, in the tri-components control DTaP vaccine group, as well as two- and tri-components rDTaP with beta-glucan group, a higher monocyte count was observed 3 days after vaccination, although less than 2 times the normal range. In the MWG test, both groups showed changes less than 20% in body temperature and body weight before the after the final immunizations. Inflammatory cytokines within the muscle at the injection site on day 3 after intramuscular injection revealed no significant response in all groups. Conclusion: There were no findings associated with residual PTx, and no significant differences in both local and systemic adverse reactions in the novel rDTaP vaccine compared to existing available DTaP vaccines. The results suggest that the novel rDTaP vaccine is safe. [ABSTRACT FROM AUTHOR]

Bin microphysics schemes prognose the raindrop size distribution (RSD), which can be directly evaluated through comparison with disdrometer observations. This evaluation will provide implications on the reliability of simulated cloud microphysics by bin microphysics schemes. In this study, the RSDs of a precipitation event associated with an extratropical cyclone passing South Korea are simulated using a bin microphysics scheme and compared with those observed by a ground‐based disdrometer. The simulated mean RSD overall agrees with the observation. However, notable overestimations appear in the large‐ (3.3–4.3 mm) and small‐ (0.56–1.88 mm) diameter ranges, which respectively stem from the biases in two different time periods, one dominated by stratiform rain and the other largely involved with convective rain. In the stratiform‐rain‐dominated period, the melting of snow is the largest contributor to RSDs. The overestimation in the large‐diameter range in this period can be associated with overly active ice–ice collection at upper levels, which generates a local maximum in RSD at the diameter of 3.3 mm that is not seen in the observed RSDs. In the convective‐rain‐involved period, the warm‐rain collision–coalescence is the largest contributor to RSDs. The overestimation in the small‐diameter range and underestimation in the large‐diameter range imply that the collisional growth of raindrops is represented to be weaker than that in reality. The findings in this study suggest that the RSDs simulated using a bin microphysics scheme can have some systematic biases associated with misrepresentation of some microphysical processes. Plain Language Summary: Bin microphysics schemes are a type of cloud microphysics schemes that allow for the particle size distributions of hydrometeors to take any form at the expense of huge computational resources, aiming to represent the cloud microphysics as realistically as possible. In this study, the raindrop size distributions predicted using a bin microphysics scheme are evaluated through comparison with ground‐based observations obtained using a disdrometer. The mean raindrop size distribution is overall well reproduced, but the raindrop number concentration is overestimated in the small‐ and large‐diameter ranges. The overestimation in the large‐diameter range occurs in the period when stratiform rain is dominant, attributable to the overgrowth of ice particles via ice–ice collection and their melting into large raindrops. The overestimation in the small‐diameter range occurs in the period when convective rain is largely involved, which is attributed to the insufficient growth of raindrops via coalescence with other drops. Key Points: Raindrop size distributions simulated using a bin microphysics scheme are evaluated in comparison with disdrometer observationsFor stratiform rain, the number of large raindrops is overestimated due to overgrowth of snow particles by aggregation and their meltingWhen convective rain is largely involved, the number of small raindrops is overestimated due to insufficient warm‐rain collision–coalescence [ABSTRACT FROM AUTHOR]

Background: Previous studies have reported that genes highly expressed in leukemic stem cells (LSC) may dictate the survival probability of patients and expression-based cellular deconvolution may be informative in forecasting prognosis. However, whether the prognosis of acute myeloid leukemia (AML) can be predicted using gene expression and deconvoluted cellular abundances is debatable. Methods: Nine different cell-type abundances of a training set composed of the AML samples of 422 patients, were used to build a model for predicting prognosis by least absolute shrinkage and selection operator Cox regression. This model was validated in two different validation sets, TCGA-LAML and Beat AML (n = 179 and 451, respectively). Results: We introduce a new prognosis predicting model for AML called the LSC activity (LSCA) score, which incorporates the abundance of 5 cell types, granulocyte-monocyte progenitors, common myeloid progenitors, CD45RA + cells, megakaryocyte-erythrocyte progenitors, and multipotent progenitors. Overall survival probabilities between the high and low LSCA score groups were significantly different in TCGA-LAML and Beat AML cohorts (log-rank p-value = 3.3 × 10−4 and 4.3 × 10−3, respectively). Also, multivariate Cox regression analysis on these two validation sets shows that LSCA score is independent prognostic factor when considering age, sex, and cytogenetic risk (hazard ratio, HR = 2.17; 95% CI 1.40–3.34; p < 0.001 and HR = 1.20; 95% CI 1.02–1.43; p < 0.03, respectively). The performance of the LSCA score was comparable to other prognostic models, LSC17, APS, and CTC scores, as indicated by the area under the curve. Gene set variation analysis with six LSC-related functional gene sets indicated that high and low LSCA scores are associated with upregulated and downregulated genes in LSCs. Conclusion: We have developed a new prognosis prediction scoring system for AML patients, the LSCA score, which uses deconvoluted cell-type abundance only [ABSTRACT FROM AUTHOR]

Introduction: To understand the immune system within the tumor microenvironment (TME) of non-small cell lung cancer (NSCLC), it is crucial to elucidate the characteristics of molecules associated with T cell activation. Methods: We conducted an in-depth analysis using single-cell RNA sequencing data obtained from tissue samples of 19 NSCLC patients. T cells were classified based on the Tumor Proportion Score (TPS) within the tumor region, and molecular markers associated with activation and exhaustion were analyzed in T cells from high TPS areas. Results: Notably, tetraspanins CD81 and CD82, belonging to the tetraspanin protein family, were found to be expressed in activated T cells, particularly in cytotoxic T cells. These tetraspanins showed strong correlations with activation and exhaustion markers. In vitro experiments confirmed increased expression of CD81 and CD82 in IL-2-stimulated T cells. T cells were categorized into CD81highCD82high and CD81lowCD82low groups based on their expression levels, with CD81highCD82high T cells exhibiting elevated activation markers such as CD25 and CD69 compared to CD81lowCD82low T cells. This trend was consistent across CD3+, CD8+, and CD4+ T cell subsets. Moreover, CD81highCD82high T cells, when stimulated with anti-CD3, demonstrated enhanced secretion of cytokines such as IFN-g, TNF-a, and IL-2, along with an increase in the proportion of memory T cells. Bulk RNA sequencing results after sorting CD81highCD82high and CD81lowCD82low T cells consistently supported the roles of CD81 and CD82. Experiments with overexpressed CD81 and CD82 showed increased cytotoxicity against target cells. Discussion: These findings highlight the multifaceted roles of CD81 and CD82 in T cell activation, cytokine production, memory subset accumulation, and target cell cytolysis. Therefore, these findings suggest the potential of CD81 and CD82 as promising candidates for co-stimulatory molecules in immune therapeutic strategies for cancer treatment within the intricate TME. [ABSTRACT FROM AUTHOR]

Background: The increasing prevalence of childhood obesity has become an urgent public health problem, evidence showed that intervention for childhood obesity bring enormous health benefits. However, an effective individualized intervention strategy remains to be developed, and the accompanying remission of related complications, such as nonalcoholic fatty liver disease (NAFLD), needs to be assessed. This study aimed to develop an m-Health-assisted lifestyle intervention program targeting overweight/obese children and assess its effectiveness on indicators of adiposity and NAFLD. Methods: This is a cluster-randomized controlled trial that conducted in children with overweight/obesity in Ningbo city, Zhejiang Province, China. Students in Grade 3 (8–10 years old) were recruited from six primary schools, with three be randomized to intervention group and three to usual practice group. The intervention program will last for one academic year and consists of health education, dietary guidance, and physical activity reinforcement. This program is characterized by encouraging four stakeholders, including School, Clinic, famIly, and studENT (SCIENT), to participate in controlling childhood obesity, assisted by m-Health technology. Assessments will be conducted at baseline and 3 months, 9 months, 24 months, and 36 months after baseline. The primary outcome will be the differences between the two groups in students' body mass index and fatty liver index at the end of the intervention (9 months after baseline). During the implementation process, quality control methods will be adopted. Discussion: The program will test the effectiveness of the m-Health-assisted lifestyle intervention on children with obesity and NAFLD. The results of this study will provide evidence for establishing effective lifestyle intervention strategy aimed at childhood obesity and NAFLD and may help develop guidelines for the treatment of obesity and NAFLD in Chinese children. Trial registration: Clinicaltrials.gov NCT05482191. Registered on July 2022. [ABSTRACT FROM AUTHOR]

Bangladesh is known as one of the countries that are most vulnerable to climate change, which promotes continuous scientific attention to the changes in the regional climate of this country. This study examines the long-term changes in the climate of Bangladesh using reanalysis data during the period of 1959–2021, utilizing the linear regression method. Bangladesh has experienced top-heavy tropospheric warming, with the temperature increasing at a rate of 0.21 °C decade−1 at 300 hPa and 0.07 °C decade−1 at 850 hPa, which has led to a (statistically) significant increase in the tropospheric static stability. The increase in tropospheric stability is most pronounced in the pre-monsoon season, in which the lower tropospheric warming has not occurred. In contrast, the post-monsoon and winter seasons have experienced prominent lower tropospheric warming. In conjunction with the tropospheric warming, the troposphere over Bangladesh has also undergone moistening, indicated by a 6% increase in precipitable water during the study period. The tropospheric moistening is most prominent in the monsoon season. This study reveals that the two rainiest seasons have experienced different long-term changes in precipitation characteristics. In the pre-monsoon season, the precipitation intensity has significantly decreased by 9% during the study period, which is attributable to the stabilization of the upper troposphere and consequent decrease in the potential of deep convection. In the monsoon season, the precipitation amount has significantly decreased by 10% during the study period. This decrease has occurred exclusively in the eastern part of Bangladesh, and it is primarily attributed to the weakening of monsoonal southerly flow and consequent decrease in moisture flux convergence there. [ABSTRACT FROM AUTHOR]

Chemotherapy-induced cognitive impairment is recognized as the most typical symptom in patients with cancer that occurs during and following the chemotherapy treatment. Recently many studies focused on pharmaceutical strategies to control the chemotherapy side effects, however it is far from satisfactory. There may be a need for more effective treatment options. The aim of this study was to investigate the protective effect of exercise on cisplatin-induced neurotoxicity. Eightweek-old C57BL6 mice were separated into three group: normal control (CON, n = 8); cisplatin injection control (Cis-CON, n = 8); cisplatin with aerobic exercise (Cis-EXE, n = 8). Cisplatin was administered intraperitoneally at a dose of 3.5 mg/kg/day. The Cis-EXE group exercise by treadmill running (14-16 m/min for 45 min daily, 3 times/week) for 12 weeks. Compared to the CON group, the cisplatin injection groups showed significant decrease in body weight and food intake, indicating successful induction of cisplatin toxicity. The Cis-CON group showed significantly increased levels of pro-inflammatory cytokines including IL-6, IL-1ß, and TNF-a in the hippocampus, while the Cis-EXE group was significantly decreased in the expression of IL-6, IL-1ß, and TNF-a. In addition, compared to the CON group, the levels of synapserelated proteins including synapsin-1 and -2 were significantly reduced in the Cis-CON group, and there was a significant difference between the Cis-CON and Cis-EXE groups. Antioxidant and apoptosis factors were significantly improved in the Cis-EXE group compared with the Cis-CON group. This study suggest that exercise could be meaningful approach to prevent or improve cisplatin-induced cognitive impairment. [ABSTRACT FROM AUTHOR]

Vancomycin is a frequently used antibiotic in intensive care units, and the patient's renal clearance affects the pharmacokinetic characteristics of vancomycin. Several advantages have been reported for vancomycin continuous intravenous infusion, but studies on continuous dosing regimens based on patients' renal clearance are insufficient. The aim of this study was to develop a vancomycin serum concentration prediction model by factoring in a patient's renal clearance. Children admitted to our institution between July 1, 2021, and July 31, 2022 with records of continuous infusion of vancomycin were included in the study. Sex, age, height, weight, vancomycin dose by weight, interval from the start of vancomycin administration to the time of therapeutic drug monitoring sampling, and vancomycin serum concentrations were analyzed with the linear regression analysis of the mixed effect model. Univariable regression analysis was performed using the vancomycin serum concentration as a dependent variable. It showed that vancomycin dose (p < 0.001) and serum creatinine (p = 0.007) were factors that had the most impact on vancomycin serum concentration. Vancomycin serum concentration was affected by vancomycin dose (p < 0.001) and serum creatinine (p = 0.001) with statistical significance, and a multivariable regression model was obtained as follows: Vancomycin serum concentration (mg/l) = -1.296 + 0.281 × vancomycin dose (mg/kg) + 20.458 × serum creatinine (mg/dl) (adjusted coefficient of determination, R2 = 0.66). This prediction model is expected to contribute to establishing an optimal continuous infusion regimen for vancomycin. [ABSTRACT FROM AUTHOR]

We construct an autoregressive moving average (ARMA) model consisting of the history and random effects for the streamwise velocity fluctuation in boundary-layer turbulence. The distance to the wall and the boundary-layer thickness determine the time step and the order of the ARMA model, respectively. Based on the autocorrelation's analytical expression of the ARMA model, we obtain a global analytical expression for the second-order structure function, which asymptotically captures the inertial, dynamic and large-scale ranges. Specifically, the exponential autocorrelation of the ARMA model arises from the autoregressive coefficients and is modified to logarithmic behaviour by the moving-average coefficients. The asymptotic expressions enable us to determine model coefficients by existing parameters, such as the Kolmogorov and the Townsend-Perry constants. A consequent double-log expression for the characteristic length scale is derived and is justified by direct numerical simulation data with Ret - 5200 and field-measured neutral atmospheric surface layer data with Ret ~ O(106) from the Qingtu Lake Observation Array site. This relation is robust because it applies to Ret from O(104) to O(106), and even when the statistics of natural ASL deviate from those of canonical boundary-layer turbulence, e.g. in the case of imbalance in energy production and dissipation, and when the Townsend-Perry constant deviates from traditional values. [ABSTRACT FROM AUTHOR]

Seeking novel high-performance elastocaloric materials with low critical stress plays a crucial role in advancing the development of elastocaloric refrigeration technology. Here, as a first attempt, the elastocaloric effect of TiZrNbAl shape memory alloy at both room temperature and finite temperatures ranging from 245 K to 405 K, is studied systematically. Composition optimization shows that Ti-19Zr-14Nb-1Al (at.%), possessing excellent room-temperature superelasticity with a critical stress of around 100 MPa and a small stress hysteresis of around 70 MPa and outstanding fracture resistance with a compressive strain of 20% and stress of 1.7 GPa, demonstrates a substantial advantage as an elastocaloric refrigerant. At room temperature, a large adiabatic temperature change ( Δ T ad ) of −6.7 K is detected, which is comparable to the highest value reported in the Ti-based alloys. A high elastocaloric cyclic stability, with almost no degradation of Δ T ad after 4000 cycles, is observed. Furthermore, the sizeable elastocaloric effect can be steadily expanded from 255 K to 395 K with a temperature window of as large as 140 K. A maximum Δ T ad of −7.9 K appears at 355 K. The present work demonstrates a promising potential of TiZrNbAl as a low critical stress and low hysteresis elastocaloric refrigerant. [ABSTRACT FROM AUTHOR]

Mortality of gastric cancer is the second in cancer-associated deaths due to the lack of specific symptoms at an early stage, and thus, early detection of gastric cancer is receiving more attention. Nowadays, volatile organic compound (VOC) indicators have been found to be helpful for screening a variety of cancers. Meanwhile, hybrid VOC indicators, namely, VOCs derived from both human breath and body fluids, provide more information about health status. Nevertheless, details of VOCs in body fluid (e.g. saliva) are still unclear and tracking of these VOCs remains a challenge. In this research, 10 kinds of VOCs released from the saliva were reported to be potential indicators for gastric cancer prewarning. To track these potential indicators with high specificity, a surface-enhanced Raman scattering (SERS) sensor based on a thin layer of MIL-100 (Fe) shell-wrapped Au nanostars (Au-star) was developed, and part of the aforementioned VOC indicators (e.g., 2-butanone, eucalyptol, and isopropanol) were found to be selectively detected by the sensor. These pilot results indicate a bright future for the proposed strategy for disease screening and the design of future high-performance SERS sensors. [ABSTRACT FROM AUTHOR]

High-purity 1T′-WS2 film has been experimentally synthesized [Nature Materials, 20, 1113–1120 (2021)] and theoretically predicted to be a two-dimensional (2D) superconducting material with Dirac cones [arXiv:2301.11425]. In the present work, we further study the superconducting properties of monolayer 1T′-WS2 by applying biaxial tensile strain. It is shown that the superconducting critical temperature Tc firstly increases and then decreases with respect to tensile strains, with the highest superconducting critical temperature Tc of 7.25 K under the biaxial tensile strain of 3%. In particular, we find that Dirac cones also exist in several tensile strained cases. Our studies show that monolayer 1T′-WS2 may provide a good platform for understanding the superconductivity of 2D Dirac materials. [ABSTRACT FROM AUTHOR]

Tetratricopeptide repeat (TPR) proteins play numerous roles in plant growth and development by mediating protein–protein interactions in biological systems by binding to peptide ligands. Although genome-wide analyses of the TPR gene family in other species have been performed, its evolution and function in Cucurbitaceae remain unclear. In this study, 144 TPR genes from 11 genomes of eight Cucurbitaceae species with a heterogeneous distribution on the chromosomes were characterized. Based on the homology between Cucurbitaceae and Arabidopsis, the TPR genes were divided into four groups, and the evolutionary relationships of the Benincaceae and Cucurbitaceae tribes were also represented in a phylogenetic tree. Using the 'DHL92′ genome as a reference, an integrated chromosome map was obtained containing 34 loci, 4 of which were common to the Cucurbitaceae. Cis-regulatory element analysis showed that these elements are essential for melon development and responses to light, phytohormones, and various stresses. CmTPR tissue- and development-specific expression analysis revealed differential expression patterns under normal growth conditions. Furthermore, the CmTPR genes responded to various abiotic stressors. Overall, this study offers insights into the evolutionary history of the TPR gene family in Cucurbitaceae and provides valuable information for elucidating the potential role of CmTPR genes during development and under different stresses in melon. [ABSTRACT FROM AUTHOR]

Since the 2000s, sporadic outbreaks of whooping cough have been reported in advanced countries, where the acellular pertussis vaccination rate is relatively high, and in developing countries. Small-scale whooping cough has also continued in many countries, due in part to the waning of immune protection after childhood vaccination, necessitating the development of an improved pertussis vaccine and vaccination program. Currently, two different production platforms are being actively pursued in Korea; one is based on the aP (acellular pertussis) vaccine purified from B. pertussis containing pertussis toxoid (PT), filamentous hemagglutin (FHA) and pertactin (PRN), and the other is based on the recombinant aP (raP), containing genetically detoxified pertussis toxin ADP-ribosyltransferase subunit 1 (PtxS1), FHA, and PRN domain, expressed and purified from recombinant E. coli. aP components were further combined with diphtheria and tetanus vaccine components as a prototype DTaP vaccine by GC Pharma (GC DTaP vaccine). We evaluated and compared the immunogenicity and the protective efficacy of aP and raP vaccines in an experimental murine challenge model: humoral immunity in serum, IgA secretion in nasal lavage, bacterial clearance after challenge, PTx (pertussis toxin) CHO cell neutralization titer, cytokine secretion in spleen single cell, and tissue resident memory CD4+ T cell (CD4+ TRM cell) in lung tissues. In humoral immunogenicity, GC DTaP vaccines showed high titers for PT and PRN and showed similar patterns in nasal lavage and IL-5 cytokine secretions. The GC DTaP vaccine and the control vaccine showed equivalent results in bacterial clearance after challenge, PTx CHO cell neutralization assay, and CD4+ TRM cell. In contrast, the recombinant raP vaccine exhibited strong antibody responses for FHA and PRN, albeit with low antibody level of PT and low titer in PTx CHO neutralization assay, as compared to control and GC DTaP vaccines. The raP vaccine provided a sterile lung bacterial clearance comparable to a commercial control vaccine after the experimental challenge in murine model. Moreover, raP exhibited a strong cytokine response and CD4+ TRM cell in lung tissue, comparable or superior to the experimental and commercial DTaP vaccinated groups. Contingent on improving the biophysical stability and humoral response to PT, the raP vaccine warrants further development as an effective alternative to aP vaccines for the control of a pertussis outbreak. [ABSTRACT FROM AUTHOR]

Modern mobile technologies that allow the quick creation of short videos provide users with ample opportunities to present their information in public space. Teenagers have become the most active users of new forms of creativity, so their self-presentations on video platforms deserve attention. This study uses content analysis to examine the features of Chinese adolescents' selfpresentation on Douyin, the Chinese TikTok counterpart, and the cultural factors that determine these features. We discovered that teenagers' self-presentation is characterized, firstly, by the creation of a virtual identity based on the real one, secondly, by freedom in the choice of role behavior, and thirdly, by the idealization of their image and various means of its formation. In addition to the impact of social, commercial, and network values, we also found the influence of traditional Chinese culture. Cultural stereotypes formed by upbringing are reflected in the forms of self-presentation (from external style to way of thinking) by the modern younger generation on social networks. We noted the role of celebrity culture in the daily lives of teenagers. In the future, studying the role of Chinese adolescents in the social information system, especially at the stage of changes in their self-perception and self-presentation, seems relevant. [ABSTRACT FROM AUTHOR]

This article explores the role of nucleotide metabolism in tumor microenvironments and its impact on antitumor immunity. It discusses the importance of nucleotides in cell proliferation, tumor metastasis, and therapeutic resistance. The authors also examine the role of long non-coding RNAs, copper metabolism, and autophagy in nucleotide metabolism and tumor development. The article suggests that targeting nucleotide metabolism could be a promising strategy for preventing and treating cancer, particularly through drugs that target the adenosine pathway. The authors emphasize the need for personalized approaches due to the complex interactions between tumor cells and immune cells in relation to nucleotide metabolism. [Extracted from the article]

Objectives: The prevalence of unruptured intracranial aneurysms (UIAs) in the acute ischemic stroke (AIS) cohort is probably higher than in the general population. This study investigated the prevalence of UIAs in AIS patients and the management risk and prognosis when treating AIS. Methods: From January 2020 to January 2023, we conducted a single-center retrospective study at Tianjin Huanhu Hospital. Each patient underwent both brain MRI and MRA/CTA to diagnose AIS and UIAs. Clinical, radiologic, and therapeutic data during hospitalization and prognosis were analyzed. Propensity-score matching (PSM) was performed to evaluate the risk of in-hospital adverse events, unfavorable outcomes at discharge when receiving post-stroke treatment and stroke recurrence. Results: In all, 2,181 AIS patients were included, of whom 270 had UIAs (12.4%; 95%CI 11.0–13.8%). From the unmatched and matched cohort, the incidence of in-hospital adverse events and unfavorable outcomes at discharge in patients with UIAs were not significantly different; the risk of stroke recurrence was significantly higher in patients with UIAs than in those without (unmatched: aHR, 1.71 [1.08–2.70]; matched: aHR, 2.55 [1.16–5.58]). Multivariable Cox regression models showed that aneurysm size and the presence of homoregional infarction associated with higher risk of recurrence (unmatched: aHR, 1.31 [1.21–1.41] and aHR, 3.50 [1.52–8.10]; matched: aHR, 1.28 [1.18–1.40]; p <  0.001 and aHR, 3.71 [1.12–12.34]). Conclusion: The UIAs may not increase the risk of in-hospital adverse events and unfavorable outcomes at discharge in receiving post-stroke treatment, but it may associated with a high risk of stroke recurrence. [ABSTRACT FROM AUTHOR]

Introduction: Cyclops lesions or arthrofibrotic nodules of the knee, first described by Jackson and Schaefer, occur in the anterior aspect of the intercondylar notch. Arthroscopically, they appear as head-like fibrous lesions with reddish-blue discoloration areas and consist of a pedunculated nodule of fibrovascular proliferative tissue, with or without bone or cartilaginous tissue. The cyclops lesion is a localized arthrofibrotic nodule most commonly seen following anterior cruciate ligament (ACL) reconstruction but rare on posterior cruciate ligament injuries (PCL). In this study, we present a novel case of cyclops lesion from a minor PCL injury without laxity or previous surgery. Case Presentation: A 37-year-old man visited the outpatient clinic complaining of mild knee pain, intermittent locking, and extension discomfort after minor trauma. These symptoms started 3 weeks prior, during his usual baseball activity. The magnetic resonance imaging (MRI) showed an intact ACL. Still, it had a partially injured PCL, a small suprapatellar effusion, and a cyclops lesion (2.43.4-2.5 cm) in the intercondylar notch of the femur, originating from the PCL. An arthroscopy confirmed and excised a cyclops lesion originating from the PCL. The microscopic findings included congested blood vessels of irregular-sized veins and hemorrhagic necrosis within the wavy ligament tissues, which confirmed its origin from the ligament tissue. After arthroscopic excision, the patient's symptoms were relieved without laxity. Conclusions: While most of these lesions can be treated efficiently by arthroscopy, the differential diagnosis should be performed for unique cases with a lesion from minor PCL injury without laxity or previous surgery. [ABSTRACT FROM AUTHOR]

Arecoline is the primary active carcinogen found in areca nut and has been implicated in the pathogenesis of oral squamous cell carcinoma (OSCC) and oral submucous fibrosis (OSF). For this study, we conducted a stepwise review process by combining iterative scoping reviews with a post hoc search, with the aim of identifying the specific mechanisms by which arecoline initiates and promotes oral carcinogenesis. Our initial search allowed us to define the current trends and patterns in the pathophysiology of arecoline-induced OSF and OSCC, which include the induction of cell proliferation, facilitation of invasion, adhesion, and migration, increased collagen deposition and fibrosis, imbalance in immune and inflammatory mechanisms, and genotoxicity. Key molecular pathways comprise the activation of NOTCH1, MYC, PRDX2, WNT, CYR61, EGFR/Pl3K, DDR1 signaling, and cytokine upregulation. Despite providing a comprehensive overview of potential pathogenic mechanisms of OSF, the involvement of molecules functioning as areca alkaloid receptors, namely, the muscarinic and nicotinic acetylcholine receptors (AChRs), was not elucidated with this approach. Accordingly, our search strategy was refined to reflect these evidence gaps. The results of the second round of reviews with the post hoc search highlighted that arecoline binds preferentially to muscarinic AChRs, which have been implicated in cancer. Consistently, AChRs activate the signaling pathways that partially overlap with those described in the context of arecoline-induced carcinogenesis. In summary, we used a theory-driven interpretive review methodology to inform, extend, and supplement the conventional systematic literature assessment workflow. On the one hand, the results of this critical interpretive synthesis highlighted the prevailing trends and enabled the consolidation of data pertaining to the molecular mechanisms involved in arecoline-induced carcinogenesis, and, on the other, brought up knowledge gaps related to the role of the local cholinergic axis in oral carcinogenesis, thus suggesting areas for further investigation. [ABSTRACT FROM AUTHOR]

We introduce the effective field theory of two different light dark particles interacting with the standard model (SM) light states in a single vertex, termed dark sector effective field theory (DSEFT). We focus on the new light particles with spin up to 1 and being real in essence, namely, new real scalars ϕ and S, Majorana fermions χ and ψ, and real vectors Xμ and Vμ. In the framework of low energy effective field theory with QED and QCD symmetry, the DSEFT can be classified into six categories, including the scalar-scalar-SM (ϕS-SM), fermion-fermion-SM (χψ-SM), vector-vector-SM (XV-SM), scalar-fermion-SM (ϕχ-SM), scalar-vector-SM (ϕX-SM), and fermion-vector-SM (χX-SM) cases. For each case, we construct the effective operator basis up to canonical dimension 7, which will cover most interesting phenomenology at low energy. As a phenomenological example, we investigate the longstanding neutron lifetime anomaly through the neutron dark decay modes n → χϕ or χX from the effective interactions in the fermion-scalar-SM or fermion-vector-SM case. When treating the light fermion as a dark matter candidate, we also explore the constraints from DM-neutron annihilation signal at Super-Kamiokande. We find the neutron dark decay in each scenario can accommodate the anomaly, at the same time, without contradicting with the Super-Kamiokande limit. [ABSTRACT FROM AUTHOR]

The condition of a railway vehicle's wheels is an essential factor for safe operation. However, the current inspection of railway vehicle wheels is limited to periodic major and minor maintenance, where physical anomalies such as vibrations and noise are visually checked by maintenance personnel and addressed after detection. As a result, there is a need for predictive technology concerning wheel conditions to prevent railway vehicle damage and potential accidents due to wheel defects. Insufficient predictive technology for railway vehicle's wheel conditions forms the background for this study. In this research, a real-time tire wear classification system for light-rail rubber tires was proposed to reduce operational costs, enhance safety, and prevent service delays. To perform real-time condition classification of rubber tires, operational data from railway vehicles, including temperature, pressure, and acceleration, were collected. These data were processed and analyzed to generate training data. A 1D–CNN model was employed to classify tire conditions, and it demonstrated exceptionally high performance with a 99.4% accuracy rate. [ABSTRACT FROM AUTHOR]