Search

Compared with the traditional "earth pressure balance" tunneling mode, the "air-pressure assisted" mode of shield tunneling has a more complex mechanism for maintaining excavation face balance, with significant differences in ground deformation and tunneling parameters compared to conventional full-chamber earth pressure balance tunneling mode. In order to quantitatively assess the stability of the excavation face under the air-pressure assisted tunneling mode and to determine a reasonable air-pressure setting, this study proposed an evaluation method for overall excavation face stability in the air-pressure assisted mode by using the limit equilibrium method, considering the spatial surface characteristics of the slip surface. Based on the Krabbe formula, considering the layering of the strata and the influence of groundwater, the formula for calculating gas loss was modified. In addition, a comparative tunneling test was conducted under different tunneling modes in the same strata condition, comparing the overall stability, air-pressure loss, ground settlement and tunneling parameters of the excavation face. The results showed that: according to the proposed overall stability evaluation method and the gas loss calculation method, the experimental section of this project met the overall stability requirements for air-pressure assisted tunneling. The selected air compressor had sufficient power for maintaining air-pressure stability in the strata, and the air-pressure setting met the requirements of engineering safety. Compared to the full-chamber earth pressure balance mode, the total thrust and cutterhead torque in the air-pressure assisted mode were reduced by 35% and 44%, respectively, while the advancing speed increased by 173% and energy consumption decreased by 66%. The air-pressure assisted tunneling mode resulted in a higher ground loss rate and relatively larger surface settlement. [ABSTRACT FROM AUTHOR]

Vibrations induced by hard rock tunnel boring machine (TBM) construction have become an important concern affecting the environmental quality, particularly in urban environments. Herein, the vibration mechanism of TBM construction is studied by synchronously evaluating the three-dimensional vibration acceleration at different positions (such as the TBM cutterhead, rock stratum, and ground surface) based on the TBM construction subway tunnel project in Qingdao, China. At the TBM cutterhead, the acceleration value in the X direction considerably exceeds those in the Y and Z directions. The effective acceleration values in the X, Y, and Z directions are 0.007, 0.005, and 0.004 m/s2, respectively. Because of the presence of structural cracks in rock mass, the vibration influence of strongly weathered rock strata exceeds that of slightly weathered rock strata. On the ground, the acceleration values in the X and Y directions are amplified at 8.5 m away from the tunnel axis and the vibration signals of different frequencies will rebound at different distances from the tunnel axis. Although the vibration induced by TBM construction does not exceed the national specification requirements, the noise exceeds the set limit, thus necessitating noise control measures. [ABSTRACT FROM AUTHOR]

Selenium is a vital trace mineral that is crucial for maintaining regular biological processes in aquatic animals. In this study, a four-week dietary trial was carried out to assess the impact of bio-fermented selenium (Bio−Se) on the growth and immune response of Chinese mitten crabs, Eriocheir sinensis. The crabs were randomly allocated to five dietary treatment groups, each receiving a different dose of Bio−Se. The doses included 0, 0.3, 0.6, 1.5, and 3.0 mg/kg and were accurately measured in basal diet formulations. The results showed the weight gain rate (WGR), specific growth rate (SGR), and survival rate (SR) in the 1.5 mg/kg Bio−Se group were the highest, and 3.0 mg/kg of Bio−Se has an inhibitory effect on the WGR, SGR, and SR. The activities of the immune enzymes, including glutathione peroxidase (GPX), superoxide dismutase (SOD), and acid phosphatase (ACP), of the hepatopancreas were significantly (p < 0.05) increased in the 1.5 mg/kg Bio−Se group, while they decreased (p < 0.05) in the 3.0 mg/kg feeding group compared to the 0 mg/kg feeding group. The concentration of maleic dialdehyde (MDA) exhibited the opposite pattern. Similarly, the mRNA expression levels of antimicrobial peptides (ALF-1, Crus-1, and LYS), ERK, and Relish genes were also observed to be the highest in the 1.5 mg/kg Bio−Se group compared with the other groups. Furthermore, the administration of 1.5 mg/kg of Bio−Se resulted in an increase in the thickness of the intestinal plica and mucosal layer, as well as in alterations in the intestinal microbial profile and bacterial diversity compared to the dose of 0 mg/kg of Bio−Se. Notably, the population of the beneficial bacterial phylum Fusobacteria was increased after crabs were fed the 1.5 mg/kg Bio−Se diet. In conclusion, the oral administration of 1.5 mg/kg of Bio−Se improved the growth efficiency, antioxidant capabilities, immunity, and intestinal health of E. sinensis. Through a broken-line analysis of the WGR against dietary Bio−Se levels, optimal dietary Bio−Se levels were determined to be 1.1 mg/kg. These findings contribute valuable insights to the understanding of crab cultivation and nutrition. [ABSTRACT FROM AUTHOR]

Geotextile offers numerous benefits in improving pavement performance, including drainage, barrier functionality, filtration, and reinforcement. Wicking geotextile, a novel variant in this category, possesses the intrinsic ability to drain water autonomously from soils. This paper details the development and application of a comprehensive multiphysics model that simulates the performance of wicking geotextile within a pavement system under freezing climates. The model considers the inputs of various environmental dynamics, including the impact of meteorological factors, groundwater levels, ground heat, and drainage on the pavement system. The model was firstly validated using field data from a long-term pavement performance (LTPP) road section in the cold region. It was subsequently applied to assess the impacts of wicking geotextile if it was installed on the road section. The model simulated the coupled temporal and spatial variations in soil moisture content and temperature. The simulation results demonstrated that wicking geotextile would create a suction zone around its installation location to draw water from surrounding soils, therefore reducing the overall unfrozen water content in the pavement. The results also showed that the installation of wicking geotextile would delay the initiation of frost heave and reduce its magnitude in cold region pavement. [ABSTRACT FROM AUTHOR]

A 50-day feeding experiment was performed to investigate the effects of dietary phospholipid (PL) addition on the survival, growth, serum, and hepatopancreas lipid indexes, calcium content, and neverland (Nvd) gene expression in button-sized juvenile Chinese mitten crab (Eriocheir sinensis). Five experimental diets were formulated with increasing addition of PL (0%, 1%, 2%, 3%, and 4%), which were named PL0, PL1, PL2, PL3, and PL4, respectively. Each diet was randomly assigned to four tanks of juvenile E. sinensis (initial weight: 5.00 ± 0.55 g), with eight crabs held in each tank. The results showed that PL addition significantly increased the survival rate (SR) but just slightly increased the weight gain rate (WGR) of juvenile E. sinensis. As the PL addition level increased, SR and WGR first increased and then decreased, with the highest values observed in the PL3 group. As the PL addition level increased, the activities of trypsin and cellulase significantly increased and then decreased, with the highest value observed in the PL2 group and PL3 group, respectively. As PL addition level increased from 1% to 2%, lipase activity increased sharply and then kept constant with a further increase of dietary PL. As PL addition level increased from 0% to 3%, the calcium content in the serum significantly increased from 7.96 to 12.43 mmol/L and then significantly decreased to 11.36 mmol/L with a further increase of PL addition. PL addition just slightly promoted the calcium content in the hepatopancreas compared with the control group. As PL addition level increased from 0% to 3%, the expression of Nvd in the hepatopancreas significantly increased by 1.47-fold and then decreased to basal level with a further increase of PL addition. In conclusion, 2%−3% addition of the optimal PL addition level was estimated to be 2%−3% for juvenile E. sinensis based on their survival, growth performance, and digestive enzyme activities. PL addition at a relatively higher level (3%) increased the calcium content and Nvd expression, the latter possibly facilitating cholesterol metabolism into vitamin D. [ABSTRACT FROM AUTHOR]

For the complex problem of severe tool wear encountered by shield machines tunneling in full-section hard rock, the relationship between disc cutter installation radius, stratigraphic parameters, tunneling distance, and cutter wear was analyzed by multiple regression analysis, take into account the example of shield project from Chaoyanglu Station to Emeishanlu Station of Qingdao Subway Line 6. Considered various variables such as shield thrust, rotation speed, cutterhead torque, advance speed, installation radius of cutters, UCS and CAI of rock, a face cutter wear prediction model was established to analyze the cutter's wear characteristics, and the following conclusions were drawn: (1) Within the 800 m tunneling distance, the average number of cutter changed per position was 3. 18, and as the installation radius of hob increased. (2) the average wear per linear meter of the cutter increases power exponentially with the increase of rock strength and exponentially with the increase of abrasiveness index, and the fitted correlations coefficient were high, which indicates that the above parameters play a crucial role in cutter wear. (3) the cutter wear calculation model takes into account the tunneling parameters, rock strength and abrasiveness of the rock, can predict the wear pattern of the face cutters of shields in hard rock. In addition, the accuracy of the cutter wear prediction model was verified, and the error between the predicted and measured values do not exceed 13%. [ABSTRACT FROM AUTHOR]

The vibration generated during the construction of subway tunnels with double-shield tunnel boring machine (TBM) has a significant impact on the environment, which has caused multiple complaints from residents. Taking a double-shield TBM tunnel project as the background, vibration measurements were conducted by installing vibration sensors on-site. By combining theoretical methods—such as normalization, polynomial fitting prediction, and gray correlation analysis—the vibration characteristics, impact range on the environment, and factors affecting the vibration of TBM construction were studied. The key research results included: (1) The amplified zone of X and Y vibration acceleration occurred on the left-hand side of the tunnel from 3.15 to 13.85 m, but rapidly decayed away from the amplification zone. (2) The impact range of TBM vibrations on residential areas at night and during the day was studied according to the official "Urban Regional Environmental Vibration Standard" and it was found to be larger at night than during the day. (3)The main factors affecting the TBM vibration level was studied—including the cutter-head torque, TBM thrust, cutter-head speed, penetration, field penetration index (FPI) and so on. In summary, when the double-shield TBM construction tunnel is adjacent to residential areas, the vibration generated exceeds the national standard limit. In order to reduce the impact of TBM vibration on residential areas, excavation parameters such as cutter head torque, TBM thrust, cutter head speed, and penetration should be appropriately reduced. [ABSTRACT FROM AUTHOR]

In cold regions, the environment dynamics lead to variations of soil temperature, water content, and deformation, which are characterized by highly coupled physical interplay. The hydraulic and thermal properties of unsaturated soils are highly nonlinear, which is further complicated when subjected to freezing. This paper presents a comprehensive multiphysics coupling model to evaluate these complex processes. The model considers the behaviors of unsaturated frozen soils. It accounts for the influences of meteorological, geothermal, and hydrological factors. The model is validated through two pavement case studies using Long-Term Pavement Performance (LTPP) road section data. The first case analysis is performed for a pavement section in Vermont, and the simulation lasted for 30 days during a non-freezing season on an hourly basis. The results validated the performance of the model considering unsaturated soil behaviors. The second case study is based on a daily analysis of a pavement section in South Dakota over a freezing–thawing cycle over 194 days. The results validated the model in considering the frozen unsaturated soil behaviors. Both case studies demonstrate the performance of this comprehensive model in quantifying the spatial and temporal variations of soil temperature and water content in response to environmental stressors. The capability of the model in accurately predicting the responses of pavement to the meteorological factors unleashes the potential of this model to assess the effects of climate and climate change on cold region pavement, as well as other types of geo-structures. [ABSTRACT FROM AUTHOR]

The motivation of this paper is to propose a new construction method with better control effects and economic advantages to overcome the disturbance problem in constructing small-spaced twin tunnels under narrow site conditions. First, the reinforcement measures commonly used in some typical projects in recent years and their disadvantages are summarized. Next, combined with the construction case of a double-line shield tunnel with a distance of 0.69 m from the Tel Aviv subway in Israel, a construction scheme for building an underground diaphragm wall between the two tunnels is proposed. Furthermore, the deformation and internal force change characteristics of the leading tunnel segment during the lagging shield tunneling are studied, and the interaction mechanism between small-spaced tunnels is explained. Computational results indicate that when the clearing distance between the tunnels increased from 0.096 to 0.7D, the range of segments affected by the subsequently excavated tunnel was reduced by 52%, and segments bending moment and axial force were decreased by 36% and 71%, respectively. When the tunnel depth is not greater than 2.0D, the minimum tunnel spacing should be not less than 0.28D. The required safe tunnel spacing will increase if the tunnel depth increases. Both recorded and simulated data show that an underground diaphragm wall between the tunnels can protect the leading tunnel effectively. The underground diaphragm wall was recommended for similar projects, enabling construction before shield tunneling with no need to reinforce the tunnel interior. [ABSTRACT FROM AUTHOR]

The earth pressure balance (EPB) shield tunneling efficiency is greatly affected by the choice of soil transport mode. In this study, the influence of two soil transport modes, such as the continuous belt conveyor and rail train, on the efficiency of shield excavation was analyzed using the Markov chain model. A method was proposed to define the ideal and non-ideal excavation states and quantitatively evaluate the excavation efficiency of the two soil transportation modes of the EPB shield. Based on this model framework, a profitable Markov chain model was established to predict the expected profits of the two soil transportation modes. The Beijing Metro New Airport Line first-phase project was used as a case study to verify the model established. The results show that under the same conditions, the continuous belt conveyor soil transport mode can have a higher excavation efficiency and expected profit. This advantage gradually increases over time. [ABSTRACT FROM AUTHOR]

The operation of a shield tunnel boring machine (TBM) in a high-strength hard rock stratum results in significant cutter damage, adversely affecting the thrust and torque of the cutter head. Therefore, it is very important to carry out the research on the stress characteristics and optimize the cutter parameters of cutters break high-strength hard rock. In this paper, the rock-breaking performance of cutters in an andesite stratum in the tunnel of Qingdao Metro Line No. 8 was investigated using the discrete element method and theoretical analysis. The rock-breaking processes of a disc cutter and wedge tooth cutter were simulated by software particle flow code PFC3D, and the rock-breaking degree, stress of the cutter, and rock-breaking specific energy were analyzed. The rock damage caused by the cutter in a specific section was divided into three stages: the advanced influence, crushing, and stabilizing stages. The rock-breaking degree and the tangential and normal forces of the wedge tooth cutter are larger than that of the disc cutter under the same conditions. The disc cutter (wedge tooth cutter) has the highest rock-breaking efficiency at a cutter spacing of 100 mm (110 mm) and a penetration depth of 8 mm (10 mm), and the rock-breaking specific energy is 11.48 MJ/m3 (12.05 MJ/m3). Therefore, two types of cutters with different penetration depths or cutter spacing should be considered. The number of teeth of wedge tooth cutters can be increased in hard strata to improve the rock-breaking efficiency of the shield. The research results provide a reference for shield cutterhead selection and cutter layout in similar projects. [ABSTRACT FROM AUTHOR]

The Forkhead Box O (FOXO) gene plays a key role in various biological processes, such as growth, metabolism, development, immunity and longevity. Molting is an essential process for crustacean growth, which is mainly regulated by 20-hydroxyecdysone (20E) and molt-inhibiting hormone (MIH). Although the role of FOXO in regulating the immune response of crustaceans is well documented, its involvement in controlling crustacean molting remains unclear. In this study, a FOXO-like gene (designed as EsFOXO-like) was identified in Eriocheir sinensis, and the regulation of the 20E pathway by EsFOXO-like was also investigated. The coding sequence of EsFOXO-like was 852 bp, which consisted of 283 amino acids including a conserved Forkhead (FH) domain. EsFOXO-like shared high similarity with FOXO genes from other crustaceans, and the mRNA expression levels of the EsFOXO-like gene were highest in the hepatopancreas and lowest in the hemocytes. However, transcription and protein expression of the EsFOXO-like gene were found to be up-regulated only during the pre-molt stage in the hepatopancreas, with lower expression levels observed at the post-molt stage. To explore the role of EsFOXO-like in the 20E pathway, EsFOXO-like was firstly inhibited by a specific FOXO inhibitor (AS1842856) and then through an EsFOXO-like dsRNA injection, respectively, and the results showed that the relative expression levels of EsFOXO-like were notably decreased in the hepatopancreas after both the inhibitor and dsRNA treatments. The 20E concentration, the mRNA expression levels of the 20E receptors including the ecdysone receptor (EcR) and the retinoid-X receptor (RXR) and EsmTOR transcription in the AS1842856 group or the EsFOXO-RNAi group were all significantly higher than that in the control group, while the mRNA expression level of EsMIH was significantly decreased after EsFOXO-like inhibition. To further investigate whether the EsFOXO-like acts through mTOR or not, Rapamycin was administered to inhibit mTOR activity in EsFOXO-like inhibited crabs. The results revealed a significant reduction in the concentration of 20E and the expression level of EsMIH in the AS1842856 + Rapamycin group compared to the AS1842856 + DMSO group, accompanied by an increase in EsEcR and EsRXR expression. These findings collectively suggest that EsFOXO-like regulates the 20E pathway through mTOR, which offered valuable insights into the understanding of the molting process in crustaceans. [ABSTRACT FROM AUTHOR]

This study compares nickel-aluminum (Ni-Al) and 3Cr13 steel coatings deposited on carbon steel substrates by arc spraying. The objective is to select a coating that will improve the corrosion and abrasion wear resistance of boiler heat exchanger pipes. This comparison involves the study of the microstructure, phase composition, microhardness, wear and erosion, and corrosion resistance of the coatings. The corrosion resistance was evaluated based on seawater immersion, electrochemical impedance, polarization, and galvanic corrosion tests. The results showed that the Ni-Al coating had a porosity of 6.3%, while the 3Cr13 coating had a porosity of 5.2%. The average surface roughness of the 3Cr13, Ni-Al coatings, and polished substrate were 11, 14.4, and 0.13 μm, respectively. The 3Cr13 lamellar structure coating was mainly composed of the α-Fe phase and a small amount of the CrO phase, and the Ni-16wt%Al coating included a solid solution phase and a small amount of the NiO phase. During the wear tests, the Cr13 steel coating had the highest microhardness and the best abrasion resistance at room temperature at the initial stage of friction. However, its abrasion resistance was lower than that of the Ni-Al coating after a 10-min friction test. The friction coefficients of the two coatings were almost the same at 300 °C. The corrosion resistance of the Ni-Al coating was better than that of the 3Cr13 steel coating. The current density of galvanic corrosion of the 3Cr13 coating was 108 μA m-2 and that of the Ni-Al coating was 37 μA cm-2, indicating that galvanic corrosion occurred between the substrate and the coating. This comparison showed that the Ni-Al coating could provide better high-temperature abrasion resistance and anti-corrosion performance for boiler heat exchanger piping compared with the 3Cr13 steel coating. [ABSTRACT FROM AUTHOR]

The vibration generated during the construction of subway tunnels with double-shield tunnel boring machine (TBM) has a significant impact on the environment, which has caused multiple complaints from residents. Taking a double-shield TBM tunnel project as the background, vibration measurements were conducted by installing vibration sensors on-site. By combining theoretical methods—such as normalization, polynomial fitting prediction, and gray correlation analysis—the vibration characteristics, impact range on the environment, and factors affecting the vibration of TBM construction were studied. The key research results included: (1) The amplified zone of X and Y vibration acceleration occurred on the left-hand side of the tunnel from 3.15 to 13.85 m, but rapidly decayed away from the amplification zone. (2) The impact range of TBM vibrations on residential areas at night and during the day was studied according to the official “Urban Regional Environmental Vibration Standard” and it was found to be larger at night than during the day. (3)The main factors affecting the TBM vibration level was studied—including the cutter-head torque, TBM thrust, cutter-head speed, penetration, field penetration index (FPI) and so on. In summary, when the double-shield TBM construction tunnel is adjacent to residential areas, the vibration generated exceeds the national standard limit. In order to reduce the impact of TBM vibration on residential areas, excavation parameters such as cutter head torque, TBM thrust, cutter head speed, and penetration should be appropriately reduced. [ABSTRACT FROM AUTHOR]

The cutterhead of an earth pressure balance (EPB) shield usually relies on a large torque when tunneling through a water-rich and high-permeability sand layer. Sandy soils may lead to severe wear of the cutter tools and make the screw conveyor vulnerable to spewing. In this study, we investigate the characteristics of the soil conditioned by means of foam, bentonite slurry, and carboxymethyl cellulose solution. The mechanical and friction properties of the conditioned soil were tested by the triaxial shear, Laboratoires Central des Ponts et Chaussees (LCPC), and sliding tests, under considering the influence of soil consolidation time. The test results showed that the conditioned sand presents dilatancy, and its cohesion enhances but its internal friction angle decreases. The changing trend of these properties is more prominent with the increase of consolidation time but they stabilize after 12 h. The starting torque of the shield machine in 3-h shutdown reaches 60% of the rated torque, while the starting torque in 6-h shutdown exceeds 80% of the rated torque. To avoid a large starting torque, we suggest that the cutterhead is idled for a short time within 12 h after the shield machine is shutdown. Finally, the theoretical calculation method of torque on cutterhead was developed, and the prediction by such method is in good agreement with the in situ measured data in the cutterhead. [ABSTRACT FROM AUTHOR]

This work focuses on the development of a three-dimensional shield tunneling model coupling the finite-difference method (FDM) with the discrete-element method (DEM) and evaluates its tunneling performance in boulders. The tunneling process of cutterhead rotation, cutting the formation, soil entering the chamber, and discharging of the screw conveyor is simulated to explain the characteristics of particle movement in front of the tunnel face. The range of the soil failure zone under different shield discharge ratios is determined by the correlation between the discharge ratio and the tunnel surface stability. The evolution process of the soil arching effect in the boulders along with shield tunneling is investigated to understand the mechanisms of the delay settlement in the boulders. Subsequently, the shield discharge ratio is optimized by investigating its influence on the surface deformation. Finally, the average stress distribution on the shield cutterhead in the tunneling process is analyzed to provide the scheme of the cutterhead structure and arrangement. This work can be referred for evaluating the shield tunneling performance and optimizing the excavation design scheme of shield machines during tunneling construction under similar geological conditions. The coupled finite-difference method (FDM)–discrete-element method (DEM) calculation method proposed in this study was successfully applied for boring tunnel equipment performance analysis in Beijing Metro Line 16. This shield tunnel was excavated in the strata with boulders rich in large size and high strength, and the previous tunneling experience shows that the shield is prone to encountering accidents such as tool damage and cutterhead jamming by large boulders when tunneling in such strata. Based on the present parametric analysis and calculation results, the suitable engineering suggestions have been proposed to enhance the shield equipment specifically, such as strengthening the central and peripheral cutters and the wear resistance of the cutterhead sides. In addition, the cutterhead opening and band-type screw conveyor were also adjusted to allow large size stones to pass through the cutterhead and discharge from the soil chamber as much as possible instead of being broken by the cutters. The adoption of these techniques has made it possible for the earth pressure balance shield (EPBS) machine to continuously excavate 1,300 m in boulders without cutter exchanges. [ABSTRACT FROM AUTHOR]

This paper describes the development of a random finite element model (RFEM) that allows holistic simulation of frozen soil behaviors, including the effects of phase transition and the consequent internal stress and volume changes. The performance of the model is firstly validated with laboratory experiments. The model is implemented to simulate the effects of frost action on pavement. The coupled thermal-mechanical actions including the mechanical responses of subgrade soils subjected to freezing temperature and its effects on the pavement structure are analyzed. The results show that the frost action and expansion of ice lenses change the interaction modes between pavement layers. This leads to increase of stress and deformation in the pavement layer. Methods to mitigate the effects of frost heave are analyzed with this model. The simulation results indicate that the detrimental effects of frost heave on the pavement structure can be mitigated by increasing the thickness of base layer, use of thermal insulation layer or improve drainage in the subgrade layer. The RFEM combines the advantages of discrete element model (DEM) in holistically describing the microstructure effects and in the finite element model (FEM) in terms of computational efficiency. This allows to focus research on understanding the behaviors of individual soils phase and their interfacial interactions. [ABSTRACT FROM AUTHOR]

When excavating tunnels in the clay stratum by using earth pressure balance (EPB) shields, the cutterhead is prone to clogging and may lose its ability to excavate. To investigate the clogging mechanism, a cutterhead device equipped with a driving system was developed to emulate the cutterhead behavior of the EPB shield in conditioned soils. The influences of water content, consolidation time, and soil conditioning factors on clay adhesion were evaluated and compared based on general laboratory and cutterhead model tests. The mixing test results showed that the water content influenced considerably clay adhesion. A water content interval near the liquid limit caused a decrease in the adhesion coefficient, and the change in water content was more effective than that in clay conditioning. Direct shear tests revealed that soil conditioning could reduce clay strength, and the effect was evident when the consolidation time was less than 6 h. A peeling point was observed in the adhesion curve of the clay and steel blocks. The peeling point for unconditioned clay was greater than the corresponding shear failure strength, which indicated that the clay had failed before it was detached from the steel block surface. However, contrasting results were obtained for conditioned clay. The cutterhead model test also showed that the water content had the greatest influence on clay adhesion according to the torque and thrust. [ABSTRACT FROM AUTHOR]

The present study investigates the distribution and geological characteristics of cobbles and boulders in the southwestern area in Beijing to provide a basis for the subway engineering design and construction process. An abundance of boulders with large particle sizes in the underground leads to a challenge to the construction of metro tunnels. Based on drilling exploration, the exploration shaft method is used to investigate the distribution of large-size boulders in the soil. The spatial distribution of boulders was determined, and it was found that large-size boulders were mainly distributed at burial depths of 17–19 m, with a maximum particle size of 67 cm, and the total boulder content reached a maximum of 48%. The physical and mechanical properties of the cobbles and boulders were tested in the laboratory. The results show that the size distribution of boulders at the same depth exhibits a large dispersion, and boulder strength is positively correlated with size. Furthermore, the mineral composition analysis indicates that the equivalent quartz content (EQC) is the main factor in determining the geological characteristics of the pebbles. Additionally, it is found that the EQC of the pebbles is strongly correlated with the uniaxial compressive strength and Cerchar abrasivity index value, and larger-size pebbles result in higher EQC values. [ABSTRACT FROM AUTHOR]

The construction of overlapping shield tunnels causes secondary disturbance to the ground, which will increase surface deformation. In order to study the law of surface deformation, this paper, relying on the Tianjin Metro Line 5 West Hotel Road-West Huanhu Road Interval and Line 6 West Huanhu Road-West Hotel Road Interval tunnel project, uses FLAC3D to numerically simulate the two excavation sequences and analyzes the measured data of 15 surface monitoring cross-sections to obtain the law of surface deformation of Tianjin soft soil layer overlapping shield tunnel construction. Research results indicate: (1) For the upper tunnel of this project, the reasonable range of advancing earth pressure to static earth pressure ratio α is 2.0~2.4, and the lower tunnel α should be 1. 2~1.6; (2) By fitting the measured surface data, it can be approximated that the settlement parameter k in the Peck formula obeys the distribution of N ~ (0.981, 0.230); (3) The shape and width of the surface settlement curve do not change with different excavation sequences, and the maximum surface settlement of excavation sequence 2 is increased by 0.8 mm; (4) With "first down, then up" excavation sequence, the width of the surface settlement trough is mainly affected by the lower tunnel. [ABSTRACT FROM AUTHOR]

Soil conditioning of an earth pressure balance shield in a saturated sand layer aims to impart adequate flow plasticity and impermeability. Thus, the effect of soil conditioning on the saturated sand layer was analyzed for Harbin Metro Line 2 in the tunnel section project from Harbin North Station to Dagengjia Station, and the optimal soil conditioning ratio was obtained through experiments on medium sand using foam, bentonite, and carboxymethyl cellulose. In addition, the microscopic pores and shape characteristics of the soil before and after conditioning were compared using computerized tomography (CT) scanning and scanning electron microscopy (SEM) to evaluate the microscopic effect of soil conditioning. The current research results revealed that the soil conditioning reduced the particle size dispersion and improved the particle gradation, and the conditioned soil particles were more uniform than the original soil sample. Moreover, the CT scanning images processed using the threshold method illustrated that the pores of the conditioned soil were independent of each other, no continuous channels were formed, and the porosity of each layer was under 4%. In addition, the SEM images confirmed that the clay mineral composition improved the angularity and sharpness of the sand particles, and the flocculent aggregate structure, formed in combination with the sand particles, reduced the soil permeability. Furthermore, the practical application of the optimal ratio of conditioning materials, obtained experimentally and verified through the aforementioned observation, yielded proper results in the field construction, and the spewing of the screw conveyor during the shield tunneling process was avoided. [ABSTRACT FROM AUTHOR]

When the shield method is used in the upper soft and lower hard strata, it is very difficult to successively predict the ground settlement of a double line shield tunnel. By considering the Xuewa section of the Qingdao Metro, this paper describes the establishment and correction of the Peck equation for predicting the surface settlement of a double shield tunnel. The modified Peck equation was validated using numerical simulation and field monitoring methods, and the influence of the earth chamber pressure and grouting pressure on the surface settlement was analyzed. It was found that the modified Peck equation can better analyze the law of ground deformation caused by shield construction in the upper soft and lower hard strata. The ground settlement can be reduced by appropriately increasing the earth chamber pressure and grouting pressure in the numerical simulation. The results obtained by this study can provide a scientific reference for similar tunnel construction in relevant areas in the future. [ABSTRACT FROM AUTHOR]

In order to effectively control the ground deformation and ground subsidence during subway construction, three-dimensional numerical simulation of soil deformation during shield tunnel construction is proposed. Based on a subway tunnel project, this essay firstly divides the shield construction process into several stages and analyzes the vertical displacement of soil in each stage. FLAC 3D was used for three-dimensional finite difference numerical simulation. By comparing the numerical simulation results with the field measured data, the soil settlement caused by shield tunnel excavation is studied deeply. The simulation results show that the maximum settlement value of the monitoring data is 0.59 mm, and the maximum settlement value of the numerical simulation is 0.82 mm, with a difference of 0.23 mm. The maximum value of uplift on both sides of the tunnel is 0.41 mm in monitoring data and 0.29 in numerical simulation, with a difference of 0.12 mm. The maximum settlement value of monitoring data is 2.59 mm, and the maximum settlement value of numerical simulation is 3.05 mm, with a difference of 0.46 mm. The maximum value of uplift on both sides of the tunnel is 0.32 mm in monitoring data and 1.89 mm in numerical simulation, with a difference of 1.57 mm. The settlement value of numerical simulation is slightly larger than that of monitoring data. Conclusion. The simulation can well simulate the state of soil uplift on both sides, and the width of settlement groove is in good agreement with the monitoring data. [ABSTRACT FROM AUTHOR]

Double-shield TBM is more efficient than shield tunnel construction in hard rock strata. It is widely used in subway tunnel construction, such as in Qingdao and Chongqing in China. However, construction vibration problems caused by double-shield TBM critically affect human comfort in surface buildings. In order to study the vibration influence law of double-shield TBM, the on-site monitoring plan of double-shield TBM is developed, relying on a tunnel section of Qingdao Metro Line 4. Then, the vibration monitoring results are analyzed, and the time domain and frequency domain characteristics of vibration acceleration are obtained. Subsequently, taking vibration peak acceleration as the research object, the transverse and longitudinal vibration laws of double-shield TBM construction are studied. Finally, according to the standard of human comfort in buildings, the vibration influence ranges of double-shield TBM construction are obtained. The research results can provide a reference for the vibration effect of double-shield TBM passing through houses, highways and bridges. Moreover, it can also provide a basis for the disputes that double-shield TBM tunnel construction affects the lives of inhabitants. [ABSTRACT FROM AUTHOR]

Although many calculation methods for tunnel face support pressure have been proposed, they are difficult to use in practical applications. In tunneling, the tunnel support pressure is currently being determined by empirical methods. In this study, five special geological sections were selected based on the shield tunnel project between Wangfujing and Qianmen Stations of Beijing Metro Line 8 to evaluate the different support pressure ratios λ by using the particle flow code (PFC). Both the stress distribution in the ground and the settlement under different support states were analyzed. A simplified limit-equilibrium method was developed in combination with the limit state in the PFC model. The present method was compared with the classical limit-equilibrium calculation methods to predict the active limit support pressure in the tunnel face. The values of the minimum limit support pressure ratio λmin of the tunnel face were suggested. The results showed that such ratio should be set to 0.6–0.7, which implies that the change in the support pressure cannot be less than 0.6 times of the true strata stress before excavation. The present calculation method of the limit support pressure is simple and effective and can be used in the design and construction of shield tunnel engineering. [ABSTRACT FROM AUTHOR]

A 56‐day feeding experiment was performed to investigate the effects of the dietary lipid level on the survival, growth performance, digestive enzyme activities and antioxidant capacity of Eriocheir sinensis reared under three salinities. Six feeds with graded levels of lipids (5%, 7%, 9%, 11%, 13% and 15%) were formulated by including fish oil as the main lipid source. Sixty crab individuals (initial weight: 0.21 ± 0.02 g) were assigned to each aquarium. In total, 18 aquariums were distributed under each salinity condition. Each feed was randomly allocated to three parallel tanks of crabs. The feeding experiment lasted for 56 days. Results showed that the survival rate of E. sinensis fed diets with 7%–15% lipids just showed a decreasing tendency as the salinity increased. The highest weight gain rate (WGR) was found in the treatment with 11%, 9% and 5% lipid at the salinity of 0.5‰, 2‰ and 6‰ respectively. At the salinity of 0.5‰, 2‰ and 6‰, the highest activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase) were detected in the treatments with 11%, 13% and 7% lipid respectively. As the salinity increased, the protein and lipid contents showed an increasing trend, while the moisture content showed an opposite trend. At each salinity, arachidonic acid and docosahexaenoic acid increased, while saturated fatty acids and monounsaturated fatty acids decreased with the increase of dietary lipid level. These results indicated that increasing salinity resulted in decreased antioxidant enzyme activities and increased malondialdehyde content in the juvenile E. sinensis, especially when they were fed diets with relatively higher lipid level. Based on WGR, digestive enzyme activity and antioxidant capacity, the optimal lipid level was estimated to be 8.28–13%, 7.54–13% and 5–8.89% for juvenile E. sinensis reared at the salinity of 0.5‰, 2‰ and 6‰ respectively. [ABSTRACT FROM AUTHOR]

Synchronous grouting has a very important effect on shield tunnel, which directly affects the stability of excavation face and the safety of segment structure. Testing the setting time and early strength of mortar is conducive to timely control the surface settlement and prevent the segment from floating. The flow table test can ensure that synchronous grouting can fill the excavation gap of shield machine to the greatest extent. Oedometer test can obtain physical and mechanical indexes such as elastic modulus, Poisson's ratio, compression and drainage of slurry at different ages. The test results showed that the use of well-graded, small-sized machine-made gravel (0–5 mm) and natural sand (0–2 mm) with high-efficiency additives promoted the formation of early strength in grout and reduced the probability of plugging in the grouting pipe at the tail shield. The oedometer test results indicated that the quantity of water drained from a grout mixed for more than 2 h was stable and the grout deformation within 1 h of compression accounted for 80% of the total deformation. The Young's modulus of the grout reached 10 MPa when the grouting pressure was > 0.2 MPa, meeting the requirements for field injection. In this study, an efficient synchronous grouting mixture proportion suitable for calcareous sand layer is proposed, and the performance of slurry in fresh state is obtained through a variety of tests, which can be widely used in shield tunnels in similar strata. [ABSTRACT FROM AUTHOR]

It is important to enable the prediction of disc cutter wear during shield tunnelling through hard rock because such wear is associated with project delays and increased costs. Based on a theoretical analysis of Archard's wear mechanism and Euler's rotation theorem, a displacement equation of rock-breaking point A on the disc cutter ring is studied, and then a new wear prediction model is established combined with the theoretical analysis of the disc cutter force and wear test. The cutter wear prediction model is verified by using the data of two cases, and the results reveal that the errors between measured and predicted values are less than 25%. The research results also show that abrasive wear is the main wear mechanism (approximately 88%). In addition, the influence of fatigue wear is very small, accounting for only approximately 2%, which can be ignored. Finally, the maximum tunneling distance of disc cutters under different installation radii and penetration is studied using the model, and the results can be used as references for optimizing shield tunneling parameters and predicting the disc cutter replacement time. [ABSTRACT FROM AUTHOR]

Objectives: From the viewpoint of ultrasound (US) physicians, an ideal thyroid US computer-assisted diagnostic (CAD) system for thyroid cancer should perform well in suspicious thyroid nodules with atypical risk features and be able to output explainable results. This study aims to develop an explainable US CAD model for suspicious thyroid nodules.Methods: A total of 2992 solid or almost-solid thyroid nodules were analyzed retrospectively. All nodules had pathological results (1070 malignancies and 1992 benignities) confirmed by ultrasound-guided fine-needle aspiration cytology and histopathology after thyroidectomy. A deep learning model (ResNet50) and a multiple risk features learning ensemble model (XGBoost) were used to train the US images of 2794 thyroid nodules. Then, an integrated AI model was generated by combining both models. The diagnostic accuracies of the three AI models (ResNet50, XGBoost, and the integrated model) were predicted in a testing set including 198 thyroid nodules and compared to the diagnostic efficacy of five ultrasonographers.Results: The accuracy of the integrated model was 76.77%, while the mean accuracy of the ultrasonographers was 68.38%. Of the risk features, microcalcifications showed the highest contribution to the diagnosis of malignant nodules.Conclusions: The integrated AI model in our study can improve the diagnostic accuracy of suspicious thyroid nodules and output the known risk features simultaneously, thus aiding in training young ultrasonographers by linking the explainable results to their clinical experience and advancing the acceptance of AI diagnosis for thyroid cancer in clinical practice.Key Points: • We developed an artificial intelligence (AI) diagnosis model based on both deep learning and multiple risk feature ensemble learning methods. • The AI diagnosis model showed higher diagnostic accuracy for suspicious thyroid nodules than ultrasonographers. • The AI diagnosis model showed partial explainability by outputting the known risk features, thus aiding young ultrasonic doctors in increasing the diagnostic level for thyroid cancer. [ABSTRACT FROM AUTHOR]

Introduction: To evaluate the value of artificial intelligence (AI)-assisted software in the diagnosis of lung nodules using a combination of low-dose computed tomography (LDCT) and high-resolution computed tomography (HRCT). Method: A total of 113 patients with pulmonary nodules were screened using LDCT. For nodules with the largest diameters, an HRCT local-target scanning program (combined scanning scheme) and a conventional-dose CT scanning scheme were also performed. Lung nodules were subjectively assessed for image signs and compared by size and malignancy rate measured by AI-assisted software. The nodules were divided into improved visibility and identical visibility groups based on differences in the number of signs identified through the two schemes. Results: The nodule volume and malignancy probability for subsolid nodules significantly differed between the improved and identical visibility groups. For the combined scanning protocol, we observed significant between-group differences in subsolid nodule malignancy rates. Conclusion: Under the operation and decision of AI, the combined scanning scheme may be beneficial for screening high-risk populations. [ABSTRACT FROM AUTHOR]

This study evaluated survival, growth performance, digestive enzyme activities, intestinal histology, and antioxidant enzyme activities of the Pacific white shrimp (Litopenaeus vannamei), which were fed with five different diets, containing 0%, 25%, 50%, 75%, and 100% fresh black soldier fly larvae (BSFL), respectively, to replace commercial feed at an equal wet weight. The cultured experiment was lasted for 45 days, and the results showed that survival rate (SR), final body weight (FBW), and weight gain (WG) were negatively related with the replacement rate of fresh BSFL in the diet, where the maximum value was found in the BSFL 25% group, while the minimum value appeared in the BSFL 100% group. When BSFL replacement level was equal to or above 75%, the SR, FBW and WG were significantly decreased. However, hepatosomatic index (HSI) was increased with the increasing BSFL replacement level, which was significantly higher in BSFL 75% and BSFL 100% groups than the other groups. No significant differences on hepatopancreatic amylase and lipase activities of the shrimp were observed among all the groups. However, compared with the control group, protease activity in hepatopancreatic of the shrimp was significantly higher when up to 25% of commercial feed was replaced. The histological structure of the intestine gradually changed pathologically, such as tissue disruption, with increasing proportion of fresh BSFL in the diets. A significant reduction in intestinal fold height was found in the BSFL 100% group, and a decreased thickness of intestinal muscular was also observed in all treatment groups. The serum SOD and GSH-PX activities of shrimp in all treatment groups were significantly higher than that of the control group. In conclusion, replacing commercial feed up to 50% with fresh BSFL could be feasible for L. vannamei farming when growth performances, digestive enzyme activities, intestinal histology, and antioxidant enzymes were being considered. [ABSTRACT FROM AUTHOR]

Magnetoactive membrane-type acoustic metamaterials are fabricated by coating a layer of magnetic nanoparticles on the polyethylene (PE) membranes and their vibration characters are investigated experimentally. From our experiments, we discovered that, under different magnetic fields by varying the distance between a magnet and the membranes, such membranes exhibit tunable vibration eigenfrequencies (the shift towards lower frequencies), which is caused by the variation of the effective mass density and effective tension coefficient resulted from the second derivative of the magnetic field. The strong magnetic force between the layer of magnetic nanoparticles and the magnet enhances the eigenfrequency shift. A spring oscillator model is proposed and it agrees well with the experimental results. We also experimentally observed that the vibration radius, effective mass density, and effective tension coefficient of the membranes can enormously affect the eigenfrequencies of the membranes. We believe that this type of metamaterials may open up some potential applications for acoustic devices with turntable vibration properties. [ABSTRACT FROM AUTHOR]

A 60‐day feeding experiment was performed to investigate the survival, growth, body composition, antioxidant capacity and related gene expression of Eriocheirsinensis which were fed diets with different lipid sources under three salinities. Five feeds were formulated with one of the candidate oils (fish oil, soybean oil, linseed oil, colza oil or palm oil) and were named FO, SO, LO, CO or PO respectively. Results showed that the survival rate (SR) of E. sinensis showed a decreasing tendency as the salinity increased. But SR was not significantly affected by dietary lipid sources at each salinity. Weight gain rate (WGR) were highest in the treatment of FO/PO at a salinity of 0.5‰, CO/FO/SO at a salinity of 2‰ and LO at a salinity of 6‰ respectively. At salinities of 0.5‰ and 2‰, the transcription of pro‐inflammatory and antioxidant genes in LO was significantly higher than that in FO or PO. While at a salinity of 6‰, the transcription of detected genes in FO was markedly increased compared to that in the vegetable oil treatments. At each salinity, the eicosapentaenoic acid (EPA) contents showed comparable values among different dietary treatments. However, the docosahexaenoic acid (DHA) contents in E. sinensis fed the diets with FO were still significantly higher than those with vegetable oils. These results showed that increasing salinity decreased the antioxidant capacity and therefore negatively affected the survival and growth rates of experimental animals. The beneficial effects of FO on survival and growth performance were eliminated by increasing the salinity to 6‰, which could be due to the increased lipid peroxidation and inflammatory response caused by salinity stress. It was concluded that at the salinity of 0.5‰ and 2‰, the recommended lipid sources were FO/PO and CO/FO/SO respectively. While at the salinity of 6‰, LO was the most ideal lipid source for juvenile E. sinensis. [ABSTRACT FROM AUTHOR]

Background: Acute respiratory distress syndrome (ARDS) is a heterogeneous syndrome, and the identification of homogeneous subgroups and phenotypes is the first step toward precision critical care. We aimed to explore whether ARDS phenotypes can be identified using clinical data, are reproducible and are associated with clinical outcomes and treatment response.Methods: This study is based on a retrospective analysis of data from the telehealth intensive care unit (eICU) collaborative research database and three ARDS randomized controlled trials (RCTs) (ALVEOLI, FACTT and SAILS trials). We derived phenotypes in the eICU by cluster analysis based on clinical data and compared the clinical characteristics and outcomes of each phenotype. The reproducibility of the derived phenotypes was tested using the data from three RCTs, and treatment effects were evaluated.Results: Three clinical phenotypes were identified in the training cohort of 3875 ARDS patients. Of the three phenotypes identified, phenotype I (n = 1565; 40%) was associated with fewer laboratory abnormalities, less organ dysfunction and the lowest in-hospital mortality rate (8%). Phenotype II (n = 1232; 32%) was correlated with more inflammation and shock and had a higher mortality rate (18%). Phenotype III (n = 1078; 28%) was strongly correlated with renal dysfunction and acidosis and had the highest mortality rate (22%). These results were validated using the data from the validation cohort (n = 3670) and three RCTs (n = 2289) and had reproducibility. Patients with these ARDS phenotypes had different treatment responses to randomized interventions. Specifically, in the ALVEOLI cohort, the effects of ventilation strategy (high PEEP vs low PEEP) on ventilator-free days differed by phenotype (p = 0.001); in the FACTT cohort, there was a significant interaction between phenotype and fluid-management strategy for 60-day mortality (p = 0.01). The fluid-conservative strategy was associated with improved mortality in phenotype II but had the opposite effect in phenotype III.Conclusion: Three clinical phenotypes of ARDS were identified and had different clinical characteristics and outcomes. The analysis shows evidence of a phenotype-specific treatment benefit in the ALVEOLI and FACTT trials. These findings may improve the identification of distinct subsets of ARDS patients for exploration in future RCTs. [ABSTRACT FROM AUTHOR]

A 60‐day feeding experiment was conducted to evaluate the survival, growth, antioxidant capacity and body composition of the juvenile Chinese mitten crab (Eriocheir sinensis) reared at three salinities (0.5‰, 2‰ and 6‰) in response to the different substitution levels (0%, 24%, 39%, 57% and 100%) of fish meal (FM) by soybean meal (SBM). The five diets were named as S0, S24, S39, S57 and S100 respectively. Results showed that survival rate (SR) was only significantly affected by the substitution level at the salinity of 0.5‰, with significantly higher SR observed in S39 (p < 0.05). At the salinity of 0.5‰ and 2‰, the significantly higher weight gain rate (WGR) was observed in S39 and S57 respectively (p < 0.05). At the salinity of 6‰, WGR was significantly improved by partial or complete substitution of FM by SBM. At each salinity, the highest GSH‐Px activity and lowest MDA content were observed in the treatments of S57, which were comparable to that fed S0. At the salinity of 6‰, the activities of SOD and CAT were significantly decreased in S100 than that in the other treatments (p < 0.05). The proline content significantly increased as the substitution level increased at the salinity of 2‰. At the salinity of 6‰, the contents of proline, glutamine, cysteine, methionine and lysine, first significantly increased and then decreased (p < 0.05), with the highest value observed in the treatment of S39 or S57. These results indicated that the optimal substitution level of FM by SBM was estimated to be 39% and 57% in the diets of E. sinensis at the salinity of 0.5‰ and 2‰ respectively. Although the FM can be completely replaced by SBM without negatively affecting the survival and growth performance at the salinity of 6‰, the antioxidant capacity was reduced to a large extent. [ABSTRACT FROM AUTHOR]

Cutting tool wear in tunnel boring machine (TBM) affects the construction safety, time, and cost in tunneling engineering. To accurately calculate the wear of TBM cutting tools used in heterogeneous grounds, the wear coefficient was studied theoretically and using field experiments based on the tool wear model proposed by the Japanese Tunnelling Society (JTS). A trajectory weight-based wear coefficient was proposed along with a calculation method that enhanced the model's adaptability to heterogeneous grounds. The conclusions were as follows: (1) The wear coefficients for cutting tools installed at different installation radii had to be calculated separately due to their dissimilar values in heterogeneous grounds. (2) Analysis of measured data proved that the distribution of strata in the excavation area, the wear coefficient of each ground, and the cutting tools installation radius all significantly influenced the heterogeneous ground wear coefficient. A comparison of results obtained using different calculation models showed that the model proposed in this paper was more suitable for the calculation of tool wear in heterogeneous soil strata. (3) When the JTS model and its optimization model were used, the wear of high and low cutting tools had to be calculated separately due to dissimilar wear. (4) Based on the example analyses, the wear coefficients for high rippers were 102.31–113.73 μm/km, for low rippers were 35.94–42.55 μm/km, and for scrapers were 38.60–40.34 μm/km. The wear coefficient of the low ripper and scraper was approximately 40% of that of the high ripper, and the correction coefficient for the outermost scraper and ripper was 1.2–1.4 and 2–3, respectively. [ABSTRACT FROM AUTHOR]

Increasing number of landslides occurred in the cold regions over the past decades due to rising temperature or forest fires associated with climate change. The instability of thawing slopes caused serious damages to transportation infrastructure, residential properties, and losses of human lives. These types of landslides, however, are difficult to analyze by the traditional limit equilibrium methods due to the coupled thermos-hydro-mechanical multiphysics processes involved. This paper describes a novel microstructure-based random finite element model (RFEM) to simulate the stability of permafrost slope subjected to climate change, including the increasing extent of thawing by warm atmosphere and thermal load due to forest fire. The properties of frozen soil are captured with random finite element model incorporating soil-phase coding. The thermomechanical responses of a permafrost slope are simulated to obtain the temperature, displacement, and stress fields. From these, the local factors of safety are obtained, which predict failure slumps along the slope that are consistent with the field-observed failure behaviors in permafrost slopes. The effects of climate change on the permafrost slope stability are analyzed for the years of 1956, 2017, and 2045. The results demonstrated appreciable amount of effects of climate change on the extent of slope failure zones. Forest fire led to melting of frozen soil and also affects the slope stability primarily in the shallow depth. [ABSTRACT FROM AUTHOR]

A 50‐day feeding experiment was conducted to investigate the effects of dietary protein level (26%, 34%, 43% and 52%) on the survival, growth performance, digestive enzyme activities, antioxidant capacity and body composition in the Chinese mitten crab (Eriocheir sinensis) reared at three salinities (0.5‰, 2‰ and 6‰). Survival rate (SR) showed a clearly decreasing tendency as the salinity elevated. The highest weight gain rate (WGR) was achieved in the treatment of 43% protein at the salinity of 0.5‰ and 2‰, and 26% protein at the salinity of 6‰ (p > 0.05). However, there was no significant interactive effect between dietary protein level and salinity on the SR and WGR of E. sinensis (p > 0.05). As salinity increased, the amylase activity and lipid deposition increased but the Na+/K+‐ATPase activity decreased. The MDA content of E. sinensis was increased at the salinity of 2‰ than the other two salinities by low or moderate dietary protein (26–43% dry diet), but was significantly decreased by high protein (52% dry diet). Correspondingly, the activities of GSH‐Px were increased at the relatively higher protein (43–52% dry diet). The significant interactive effect was only obtained between dietary protein level and salinity on the activities of CAT, GSH‐Px and MDA content of E. sinensis. Dietary protein level had a more obvious positive effects on the EAA deposition at the salinity of 2‰ and 6‰, with the highest contents of essential amino acids (EAAs) achieved by 43% dietary protein. In conclusion, the relatively higher protein could benefit the E. sinensis by inhibiting the lipid peroxidation and increasing the deposition of EAAs at the salinity of 2‰. The elevated activities of amylase could account for the low protein (26%) requirement for E. sinensis reared at the salinity of 6‰. [ABSTRACT FROM AUTHOR]

Surface surcharge changes the existing equilibrium stress field of the stratum and adversely affects the existing tunnel. This paper presents a simplified analytical solution for calculating the longitudinal displacement of existing tunnels that are subjected to adjacent surcharge loading. Based on the Boussinesq solution, the distribution of the additional load matrix caused by the surface surcharge on the existing tunnel was obtained. A Euler–Bernoulli beam with a Pasternak foundation was used as a simplified model for tunnel stress analysis. Using the corrected reaction coefficient of the foundation bed, the differential equation of tunnel deformation was established, and the solution matrix of the longitudinal displacement of the tunnel was obtained by using the finite difference method. The reliability and applicability of the proposed method were verified by comparing the results with finite element simulation results, field test data, and the calculation results of three simplified elastic analysis methods with different foundation bed coefficients. On this basis, the parameters of the load–tunnel model were analyzed, and the effects of the buried depth, the size of the load, the relative positions of the load and the tunnel, and the relative stiffness of the tunnel soil on the maximum displacement of the existing tunnel were calculated. An empirical formula is proposed for calculating the maximum longitudinal displacement of the existing tunnel subjected to surface surcharge. The findings of this research can provide a basis for the theoretical verification of the deformation response of an existing tunnel subjected to adjacent surface surcharge. [ABSTRACT FROM AUTHOR]

In urban areas, the construction of subway tunnels is faced with complex crossing problems. The interaction of tunnels with structural deformation has not been comprehensively studied, especially in the construction of new tunnels crossing above existing tunnels. To better predict the structural deformation of the existing tunnels caused by shield excavation, this study used FLAC3D finite difference software, field monitoring, and an analytical method. A numerical model was used to simulate the influence of the shield weight, grouting pressure, and the grout hardening process on the existing tunnel. The results show that the deformation of horseshoe-shaped tunnel structure can be divided into four stages and the corresponding control measures should be taken for each stage to prevent the structural damage. Moreover, the weight of the shield can restrain the existing tunnel from floating up, but it may cause the cracking of the existing tunnel structure without internal steel support. Based on the "two-stage analysis method", the simplified analytical method solved the additional stress and the uplift deformation in the first stage and second stage, respectively. The proposed analytical method can rapidly estimate the maximum uplift deformation of the existing horseshoe-shaped tunnel with different bending stiffness values under shield excavation. [ABSTRACT FROM AUTHOR]

This paper focuses on the influence of particle size distribution, test time, and moisture content on the Laboratoire Central des Ponts et Chaussées (LCPC) abrasivity coefficient (LAC). Image analysis and X-ray diffraction were used to analyze the particle shape characteristics and equivalent quartz content (EQC) of a sandy stratum. The test results show no significant differences in the shape characteristics and EQC of particles with different diameters obtained from the same sandy stratum. For the same EQC, the main factors affecting the LAC are particle size distribution, test time, and moisture content. An optimized effective size (ESop) index was established to represent different particle size distributions. The test results showed that the ESop index is more accurate than other indices in determining the LAC of soil samples, and the LAC had a linear relationship with the ESop index. Soil samples with moisture content less than the saturated moisture content have an LAC larger than that of a dry soil sample, and soil samples with moisture content greater than the saturated moisture content have an LAC similar to that of a dry soil sample. The influence of test time on the LAC of the soil samples could be divided into two stages, where the first and second stages are represented by a quadratic function and a linear function, respectively. [ABSTRACT FROM AUTHOR]

In this study, the reasonable construction sequence of an overlapping tunnel shield is investigated. Taking the overlapping tunnel of Tianjin Metro Line 5 as the background, a three-dimensional numerical model was established using Flac3D software to study the influence of the "first up and then down" and "first down and then up" construction sequences of the overlapping tunnel on the surface settlement, stratum displacement, lining radial stress, and displacement. The research results show that the shape and width of the final settlement tank on the ground under the two conditions are basically the same, and the difference between the maximum cumulative settlements is small. The accumulated ground settlement caused by the first up and then down construction sequence is 1.8 mm larger than that caused by the first down and then up sequence. The difference between the two working conditions on the vertical displacement of the stratum mainly occurs in the middle stratum of the upper and lower tunnels, and the proportion of the strata in the uplifted state and subsidence state is different. The construction sequence has little effect on the radial stress on the lining of the upper and lower tunnels. Under these two conditions, the excavation of the second tunnel causes a small change in the radial stress of the preceding tunnel lining, both within 4.2%. Under the working condition of first down and then up, the construction of the second tunnel causes the lining of the preceding tunnel to rise by 7.2∼9.2 mm. Under the condition of first up and then down, the construction of the second tunnel causes the lining of the preceding tunnel to sink again by 9.1∼10.4 mm. By comparing the effects of the two working conditions on the stratum and the tunnel lining, it is recommended that the construction be carried out in the order of first down and then up. [ABSTRACT FROM AUTHOR]

Shield recovery in water-rich sand strata is a challenging issue in the field of shield tunnel engineering, especially when the end of the shaft cannot be reinforced by jet grouting or freezing or when the shield cannot be supported with a steel sleeve. Therefore, it is important to develop an effective recovery approach and adopt suitable techniques to control the risks. In this study, a new method based on filling the receiving shaft with water is proposed for the underwater recovery of an earth pressure balance (EPB) shield with zero end reinforcement from a metro tunnel in Tel Aviv, Israel. Several additional techniques are used to ensure safe recovery of the shield, including the design of a concrete cradle, drilling of pressure relief holes, control of excavation parameters, screw conveyor sealing, portal sealing, tail sealing, and grouting. Furthermore, according to the actual situation on site, filling the shaft with water to 1 m above the water level in the strata can prevent the fine sand from percolating into the shaft. Before the cutterhead approaches the underground diaphragm wall, the driving attitude should be strictly controlled, and the edge hob should be inspected for wear. The necessary thrust of shield tunneling in the underground diaphragm wall and shaft is calculated theoretically. In order to ensure the deformation control of the underground diaphragm wall and the smooth tunneling of the shield, the thrust of the shield excavating the underground diaphragm wall will not be larger than 12 000 kN, and the penetration degree will be limited to 3 mm/r. Qualitative observations and measurements of surface subsidence in the metro tunnel indicate that these risk mitigation techniques are effective and suitable for the underwater recovery of EPB shields in water-rich sand strata. [ABSTRACT FROM AUTHOR]

Quantifying the expression of mRNAs in the gonads at the critical stage of molecular sex differentiation stage might help to clarify the regulatory network during early sex differentiation and provide new information on the role of sex-related genes in gonadal function. In this study, transcriptomic analysis of sex-related genes expression profiles in fugu gonads at 60 and 90 days after hatching (dah) was conducted firstly, and a total of 112,504,991 clean reads, encompassing 28.35 Gb of sequences were retrieved. Twenty-three thousand eight hundred ten genes were found to be expressed in juvenile fugu gonads, and we mainly focused on the differentially expressed genes that have the potential to be involved in the gonadal sex differentiation. For 60-dah juveniles, we identified 1014 genes that were upregulated in the ovary and 1570 that were upregulated in the testis. For 90-dah juveniles, we identified 1287 genes that were upregulated in the ovary and 1500 that were upregulated in the testis. The dimorphic expression patterns of 15 genes in gonads at 30 and 40 dah were further investigate using qPCR. Cyp11b and star were expressed at higher levels in XY than in XX, while cyp11a1 and cyp19a1a were expressed at higher levels in XX than in XY at 30 dah. At 40 dah, the levels of gsdf, dmrt1, dmrt3, cyp11c1, star, and hsd3b expression were higher in XY, while the levels of foxl2, cyp19a1a, wnt9b, and foxD4 expression were higher in XX. Sox9, cyp11a1, cyp17a1, cyp17a2, and nr5a2 were expressed at similar levels in XX and XY at 40 dah. This is the first report of gonadal transcriptome of fugu at early sex differentiation stage, and our results provide an archive for further study on molecular mechanism underlying sex differentiation in this species. [ABSTRACT FROM AUTHOR]