1. Karyotype and chromosome location of characteristic tandem repeats in the pufferfish Tetraodon nigroviridis.
- Author
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Fischer, C., Ozouf-Costaz, C., Crollius, H. Roest, Dasilva, C., Jaillon, O., Bouneau, L., Bonillo, C., Weissenbach, 1J., and Bernot, A.
- Subjects
KARYOTYPES ,TETRAODON ,CHROMOSOMES ,HETEROCHROMATIN ,RECOMBINANT DNA ,FLUORESCENCE in situ hybridization - Abstract
Karyotype analysis of Tetraodon nigroviridis, a pufferfish of the family Tetraodontidae with a small compact genome (385 Mb) which is currently being investigated in our laboratory, indicates that this species has 2n = 42 chromosomes. The small chromosome size (the largest pair measuring less than 3 μm) has complicated accurate chromosome pairing based on morphology alone. DAPI staining, however, provides a banding-like pattern. Because of quantitative variations of some heterochromatin classes, the chromosome formula can not be established precisely, but is estimated to include approximately 20 meta- or submetacentric chromosomes and 22 subtelocentric chromosomes. A centromeric satellite, telomeric repeats, and the major and minor rRNA clusters have been localized unequivocally by FISH. As a result, the 28S and 5S rDNA sequences can be used as chromosome-specific probes. Copyright © 2000 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2000
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