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The research aimed to evaluate quality profiles, optimize the antioxidant activity, and total phenolic and flavonoid content in a mixture of three crude drug: turmeric, java tea, and seed-under-leaf, using the Simplex Lattice Mixture Design. Ultimately, a quadratic model was used, as it best adjusted to the experimental behavior. It predicted the optimal composition of herbal mixtures antioxidant activity, determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and Ferri Reducing Antioxidant Power (FRAP) techniques, also in Total Phenolic Content (TPC) and Total Flavonoid Content (TFC). The crude drugs were of good quality according to selected pharmacognostic quality parameters of the official Malaysian herbal monograph (MHM). The optimum responses with the highest synergistic effect were composed of 5% (w/w) of turmeric, 40% (w/w) of Java tea, and 55% (w/w) of seed-under-leaf. The values were 1796 mM TE/g, 2230 mM TE/g, 355 mg GAE/g, and 177 mg QE/g for DPPH, FRAP, TPC, and TFC, respectively. The greatest contribution of antioxidant activity was shown in that mixture as potential as a functional beverage. [ABSTRACT FROM AUTHOR]

Wheat is one of Indonesia's primary sources of carbohydrates. However, the need for wheat still depends on imports. So, developing local flour based on Indonesia's natural resources is necessary. One source of carbohydrates easily found in Indonesia is cassava (Manihot esculenta crantz). Although there are many in Indonesia, the diversification of cassava-based products is still limited. In this research, MOCAF flour was developed and produced by fermenting cassava tubers with lactic acid bacteria. The enzymatic activity during the MOCAF (Modified Cassava Flour) production process is believed to change the physicochemical properties of cassava so that MOCAF products have characteristics similar to wheat flour and are expected to meet the gap between the demand and availability of flour. Even though it can potentially substitute wheat, MOCAF flour only has about 1% protein content, influencing dough rheology. According to the problem, a process modification was done by adding the enzyme papain (PAP) as a support enzyme to improve the performance of lactic acid bacteria (LAB). In order to study the effect of the papain enzyme, variations were made on the ratio of lactic acid starter: papain enzyme (LAB: PAP) and fermentation time. The ratio of (LAB: PAP) was varied to 40:1, 40:3, 40:5, 40:7, and 40:9, while the fermentation period was varied to 24, 48, 72, 96, and 120 hours. The fermentation was conducted by using cassava varieties of Singkur aerobically. The primary starter contained Lactobacillus, sp, developed by PT. Rumah Mocaf Indonesia and the papain enzyme used have an activity of 100,000 U/g. Data analysis showed that the addition of papain enzyme doses with a ratio to the lactic acid starter of 40:7 was able to produce MOCAF with the best characteristics: protein content, starch content, and swelling power of 3.72%, 9.99%, 16.00%, respectively. The data trend of research also showed that the number of papain enzymes only significantly affects starch content and swelling power. On the other hand, fermentation time has a significant effect on these three characteristics (protein, starch, swelling power). [ABSTRACT FROM AUTHOR]

In this study, a novel approach to prepare spray‐dried encapsulated curcumin powder was investigated. The effects of surfactants viz. Tween 80 (at 0.25 to 0.75% wt) and lecithin (at 1% wt) and of a stabilizer viz. chitosan (at 0 to 0.375% wt) on the characteristics of curcumin‐based emulsions as well as on physicochemical properties of the resulting spray‐dried encapsulated powder were determined. The optimal emulsion was noted to be the one formulated with 0.50 and 0.25% wt, respectively, of Tween 80 and chitosan (T0.50/C0.25). Spray‐dried powder prepared from the optimal emulsion was compared to that prepared from an emulsion with 0.5% Tween 80 and 0% chitosan (T0.50/C0.00), as well as that from an emulsion with 0.25% Tween 80 and 0.25% chitosan (T0.25/C0.25). Physical properties of all powders were not significantly different. However, the encapsulation efficiency of T0.50/C0.25 powder (72.28%) was significantly higher than those of T0.50/C0.00 (47.19%) and T0.25/C0.25 powder (51.61%). Ferric reducing antioxidant powers of T0.50/C0.25 and T0.25/C0.25 powders were comparable but significantly higher than that of T0.50/C0.00 powder. After reconstitution, the mean particle sizes of T0.50/C0.25 and T0.25/C0.25 remained unchanged due to the protection by chitosan. T0.50/C0.00 powder was noted to exhibit the highest bioaccessibility (89.32%) in the simulated gastrointestinal tract. Practical Application: The results of this study can be used as a guideline to develop a stable formulation of curcumin feed emulsion that can later be transformed into an encapsulated powdery form via spray drying. Such a guideline should prove useful for a company looking for a way to produce high‐quality functional ingredients and/or products from curcumin. [ABSTRACT FROM AUTHOR]

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