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Mesenchymal stromal cells (MSC) from adult bone marrow are the most commonly used cells in clinical trials. MSCs from single donors are the preferred starting material but suffer from a major setback of being heterogeneous that results in unpredictable and inconsistent clinical outcomes. To overcome this, we developed a method of pooling MSCs from different donors and created cell banks to cater clinical needs. Initially, the master cell banks (MCBs) were created at passage 1 (P1) from the bone marrow MSCs isolated from of nine different donors. At this stage, MCBs from three different donors were mixed in equal proportion and expanded till P3 to create working cell banks. Further, the pooled cells and individual donor MSCs were expanded till P5 and cryopreserved and extensively characterised. There was a large heterogeneity among the individual donor MSCs in terms of growth kinetics (90% Coefficient of variation (CV) for cell yield and 44% CV for population doubling time at P5), immunosuppressive ability (30% CV at 1:1 and 300% CV at 1:10 ratio), and the angiogenic factor secretion potential (20% CV for VEGF and71% CV for SDF-1). Comparatively, the pooled cells have more stable profiles (60% CV for cell yield and 7% CV for population doubling time at P5) and exhibit better immunosuppressive ability (15% CV at 1:1 and 32% CV at 1:10 ratio) and consistent secretion of angiogenic factors (16% CV for VEGF and 51% CV for SDF-1). Further pooling does not compromise the trilineage differentiation capacity or phenotypic marker expression of the MSCs. The senescence and in vitro tumourigenicity characteristics of the pooled cells are also similar to those of individual donor MSCs. We conclude that pooling of MSCs from three different donors reduces heterogeneity among individual donors and produces MSCs with a consistent secretion and higher immunosuppressive profile. [ABSTRACT FROM AUTHOR]

Introduction: The longevity of an endodontically treated tooth depends on fracture resistance by preserving more remaining dentin thickness. The aim of this study is to determine which file system preserves more remaining dentin thickness. Materials and Methods: Protaper universal, M-two, Protaper Next, Trunatomy, I-Race and mandibular first premolar. The removed dentin thickness during instrumentation of each file system was noted by taking the difference of RDT of pre-instrumentation and post-instrumentation with the aid of CBCT. Results and Discussion: TRN [Group-4] shows the least aggressive cutting with maximal preservation of remaining dentin thickness at 3 mm and 6 mm from the apex at both mesiodistal and buccolingual dimensions. M-two [Group-2] shows maximum removed dentin thickness at 3 mm from the apex both mesiodistal dimension and buccolingual dimension. PTU [Group-1] shows maximum removed dentin thickness at 6 mm from the apex at mesiodistal dimension. M-two [Group-2] shows maximum removed dentin thickness at 6 mm from the apex at the buccolingual dimension. Conclusion: In this study, it is concluded that the Trunatomy file system preserves more remaining dentin thickness both mesiodistally and buccolingually both 3 mm and 6 mm from the apex. [ABSTRACT FROM AUTHOR]

Three‐dimensional (3D) printing, one of the forms of additive manufacturing, has become a popular trend worldwide with a wide range of applications including food. The technology is adaptable and meets foods nutritional and sensory needs allowing meat processing to reach a sustainable level, technology addressing the food requirement of the ever‐increasing population and the fast‐paced lifestyle by reducing food preparation time. By minimizing food waste and the strain on animal resources, technology can help to create a more sustainable economy and environment. This review article discusses the 3D printing process and various 3D printing techniques used for food printing, such as laser powder bed fusion, inkjet food printing, and binder jetting, a suitable 3D technique used for meat printing, such as extrusion‐based bioprinting. Moreover, we discuss properties that affect the printability of meat and its products with their applications in the meat industry, 3D printing market potential challenges, and future trends. [ABSTRACT FROM AUTHOR]

The occurrence of leaf folder, Pycnarmon cribrata (Fabricius, 1794) (Lepidoptera: Crambidae) on the medicinal plant nirgundi, Vitex negundo Linn. has been observed in West Bengal, India. The pest damaged the foliage by folding the leaflets, feeding within and ultimately making the foliage dry and papery. However, the identity of the pest species was confirmed through morphological characteristics of male and female genitalia as well as sequence amplified product of Cytochrome c oxidase subunit I (COI) gene (GenBank No. ON545929). Biological studies revealed that the entire life cycle of the moth completed in 29.90 ± 2.42 days on average, while the range was between 25-34 days. The peak larval incidence was noticed in 16th SMW (Standard Meteorological Week) and 24th SMW with population of 12 and 15 larvae per plant during 2021 and 2022, respectively. The percent plant incidence levels ranged from 15 to 100% during the first year i.e. 2021 and 30 to 100% during the second year i.e. 2022. The correlation of mean larval population (r = −0.66) and percent plant incidence (r = −0.71) with weather parameters indicated a highly significant negative association with the maximum temperature and a significant positive correlation with maximum and minimum relative humidity. The parasitization of Choeras sp. (Braconidae: Hymenoptera) (GenBank No. OP293230) was observed on old age larvae of P. cribrata and the natural parasitization was recorded as 26% during the study period. This study provides a comprehensive information on the occurrence, nature of damage, biology, morphometry, seasonal incidence and natural enemy fauna associated with P. cribrata which will be helpful for implementation of Integrated Pest Management (IPM) strategies to curtail the pest problem on V. negundo, a valuable medicinal plant. [ABSTRACT FROM AUTHOR]

The present review article evaluates the research and progress status of Cu–Ni–Sn-based spinodal alloys focussed on the methods and technologies implemented to enhance the properties of cast Cu–Ni–Sn alloys in industrial applications, particularly in components such as bearings, bushings, propellers, and impellers that find extensive demand in the marine and automotive industry. The review introduces the limitations of employing copper-beryllium alloys in the industry and discusses how Cu–Ni–Sn alloys represent a potential alternative. Subsequently, a comprehensive overview of spinodal decomposition is provided, considering the thermodynamic aspects and the Cu–Ni–Sn ternary phase diagram. The processing of Cu–Ni–Sn alloys via traditional casting is explored with an overview of various casting techniques and ensuing microstructure-property relationships. The two significant limitations, segregation during solidification and discontinuous precipitation, are highlighted. Strategies to overcome these limitations, such as precipitation hardening, spinodal decomposition, thermo-mechanical processing, and composition design (with the effect of varying elemental additions), are further elaborated. The review summarises novel processing routes encompassing mechanical alloying, rapid solidification, powder metallurgy, and spray deposition for developing spinodal Cu–Ni–Sn alloys. Finally, the progress in fabricating Cu–Ni–Sn spinodal alloys using additive manufacturing techniques such as selective laser melting is highlighted. There are published review papers on the wrought processing of Cu–Ni–Sn alloys, so this area has not been covered here. Overall, this review article is intended to streamline an overview of the characteristics and gradual developments from cast Cu–Ni–Sn spinodal alloys to additive manufacturing-based Cu–Ni–Sn spinodal alloys. [ABSTRACT FROM AUTHOR]

To accurately analyze the fracture behavior of piezoceramics at small length scales, flexoelectricity must be considered along with piezoelectricity. Due to its dependence on size, flexoelectricity predominates at the micro‐ and nano‐length scales. Additionally, crack tips having the largest strain gradient state cause large flexoelectricity around them. Different approaches are employed in the past to model cracks computationally. However, extended isogeometric analysis (XIGA) is proven to be an accurate and efficient method. C1 continuity requirements for modeling gradients in flexoelectricity are met by non‐uniform rational B‐splines (NURBS) basis functions used in XIGA. In this work, XIGA‐based computational model is developed and implemented to study the fracture behavior of the piezoelectric‐flexoelectric domain. An in‐house MATLAB code is developed for the same. Several numerical examples are studied to ensure the efficacy and efficiency of the implemented model, and crack behavior is presented in the form of an electro‐mechanical J‐integral. The analysis is carried out to investigate how cracks behave for different flexoelectric coefficients under different electrical and mechanical loading combinations. J‐integral is also analyzed against crack parameters such as crack orientation and length. It is observed that boundary loads and flexoelectric material constants significantly influence J‐integral. Results also show a considerable amount of fracture toughening in the presence of flexoelectricity. The peak value of J‐integral is found to be reduced with an increase in the flexoelectric coefficient. A significant reduction in J‐integral, as much as 45%, is observed when the flexoelectric constant varied from 0.5 to 2 µCm−1. [ABSTRACT FROM AUTHOR]

Bronze metal matrix composites (MMCs) are futuristic materials that may find applications in automobile, aviation, and marine industries, specifically for propellers in submarines, bearings, and bushings for defence purposes. The present investigation studied the effect of Si3N4 (5, 10, 15 wt%) ceramic particles on the physical, metallurgical, and tribological behaviour of Cu-10Sn/Si3N4 MMCs. Cast rods of three composites and a base alloy were fabricated using the liquid metallurgy route. The microstructural characterisation for the cast samples was conducted using FESEM (Field Emission Scanning Electron Microscope), EDS (Energy Dispersive Spectroscopy), XRD (X-ray diffraction), and TEM (Transmission Electron Microscope), which revealed that the Cu-10Sn alloy reinforced with 5 wt% of Si3N4 had homogeneous distribution and perfect bonding of the Si3N4 with the bronze MMC. The dry sliding wear test was performed by varying parameters such as the applied load (10, 20, 30 N) and sliding velocity (1, 2, 3 m/s). The specific wear rate (SWR) increased against an increased load. However, the SWR and coefficient of friction decreased and then increased against an increasing sliding velocity due to tribolayer formation. The primary wear mechanism observed at low and high loads was severe delamination. In contrast, the wear mechanism was adhesion wear at high and low velocities. Amongst the researched samples, Cu-10Sn/5 wt% Si3N4 composites revealed the least SWR at a load of 10 N and sliding velocity of 2 m/s and hence can be recommended for manufacturing bearings and bushings in the automobile and defence industry. [ABSTRACT FROM AUTHOR]

Purpose: This paper aims to understand the incorporation of sustainability methods of construction through the study of a low-cost housing Tamil Nadu Housing Board settlement in a locality in Chennai, India. Such a study helps to arrive at proper design decisions and incorporate a participatory approach involving the residents to enable the developers, here the decision-makers of the government, cater to the local interests. Design/methodology/approach: First, a survey was conducted to understand the local conditions and people's aspirations. Along with this, the parameters pertaining to a green housing were derived and compared with the needs of the locals, to arrive at the most influencing parameters by decision-making trial and evaluation laboratory model (DEMATEL) analysis. Findings: The study finds that parameters viz. "ensuring basic services" and "social inclusivity" happen to be the causal factors that seem to influence the needs of the locals. These factors therefore can guide the future plans to redevelop the settlement or construct similar settlements in a more sustainable way. Originality/value: This paper is a unique study to combine the twin pillars of low-cost housing and sustainability, which helps in developing settlements that are conducive to the city's socio-developmental requirements as well as respectful of the natural environment. Further, the analysis conducted through DEMATEL method could essentially guide the decision-maker to tackle urban challenges in the most locally suitable and sustainable approach. [ABSTRACT FROM AUTHOR]

Although tomato seeds (an industrial by-product) are known to contain several bioactive compounds, studies describing their health effects are limited. Previously, we evidenced that aqueous extract of tomato seeds (TSE) markedly attenuated rotenone (ROT)-induced oxidative stress and neurotoxicity in Drosophila system. This study investigated the neuroprotective effect of TSE in a chronic ROT model of neurotoxicity in mice. Initially, we assessed the potential of oral supplements of TSE to modulate the levels of endogenous markers of oxidative stress in brain regions of mice. Subsequently, employing a co-exposure paradigm, the propensity of TSE (100 mg/kg bw, 3 weeks) to attenuate ROT-induced behavioral phenotype (gait abnormalities, anxiety-like state), oxidative dysfunctions and neurotoxicity was examined. We found that mice provided with TSE supplements exhibited progressive improvement in gait pattern and exploratory behavior. TSE markedly offset ROT-induced oxidative impairments, restored reduced glutathione levels, antioxidant defenses (superoxide dismutase, glutathione peroxidase) and protein carbonyls content in brain regions. Specifically, TSE effectively diminished ROT induced elevation in the activity levels of acetylcholinesterase and restored the dopamine levels in striatum. Interestingly, in mitochondria, TSE was able to restore the activity of mitochondrial complexes and redox state. Collectively, our findings in the chronic ROT model demonstrate the ability of TSE to alleviate behavioral phenotype, oxidative stress, mitochondrial dysfunction and neurotoxicity. Further studies in dopaminergic cell models are necessary to understand the precise molecular mechanism/s by which tomato seed bioactives offer significant neuroprotection. [ABSTRACT FROM AUTHOR]