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Aim: Striatin (Strn) is a scaffold protein expressed in cardiomyocytes (CMs) and alteration of its expression are described in various cardiac diseases. However, the alteration underlying its pathogenicity have been poorly investigated. Methods: We studied the role(s) of cardiac Strn gene (STRN) by comparing the functional properties of CMs, generated from Strn‐KO and isogenic WT mouse embryonic stem cell lines. Results: The spontaneous beating rate of Strn‐KO CMs was faster than WT cells, and this correlated with a larger fast INa conductance and no changes in If. Paced (2–8 Hz) Strn‐KO CMs showed prolonged action potential (AP) duration in comparison with WT CMs and this was not associated with changes in ICaL and IKr. Motion video tracking analysis highlighted an altered contraction in Strn‐KO CMs; this was associated with a global increase in intracellular Ca2+, caused by an enhanced late Na+ current density (INaL) and a reduced Na+/Ca2+ exchanger (NCX) activity and expression. Immunofluorescence analysis confirmed the higher Na+ channel expression and a more dynamic microtubule network in Strn‐KO CMs than in WT. Indeed, incubation of Strn‐KO CMs with the microtubule stabilizer taxol, induced a rescue (downregulation) of INa conductance toward WT levels. Conclusion: Loss of STRN alters CMs electrical and contractile profiles and affects cell functionality by a disarrangement of Strn‐related multi‐protein complexes. This leads to impaired microtubules dynamics and Na+ channels trafficking to the plasma membrane, causing a global Na+ and Ca2+ enhancement. [ABSTRACT FROM AUTHOR]

A model of nonlocal heat transfer at nanoscale in rigid bodies is considered. Depending on the relevance of the particular interaction’s mechanism between the heat carriers and the lateral walls, three different strategies for the setting-up of the boundary conditions are analyzed, and the consequent forms of the basic fields have been obtained, as well. From the physical point of view, the possible influence of those interactions on the unknown fields is pointed out. From the mathematical point of view, instead, the well-posedness of the problem is shown. [ABSTRACT FROM AUTHOR]

We investigate the mechanisms leading to acoustic whistling for a jet passing through a circular hole in a thick plate connecting two domains. Two generic situations are considered. In the first one, the upstream domain is a closed cavity while the downstream domain is open, leading to a class of conditionally unstable modes. In this case, the instability source lies in the recirculation region within the thickness of the plate, but coupling with a conveniently tuned resonator is needed to select the conditional instability range. In the second situation, the two regions, upstream and downstream of the hole, are considered as open, leading to a class of hydrodynamic modes where instability of the recirculation region is sufficient to generate self-oscillations without the need of any resonator. A matched asymptotic model, valid in the low Mach limit, is used to derive a global impedance of the system, combining the impedance of the hole and the modelled impedances of the upstream and downstream domains. It is shown that the knowledge of this global impedance along the real ω-axis provides an instability criterion and a prediction of the eigenvalues of the full system. Validations against the solutions of the eigenvalue problem obtained from the linearized fully compressible formulation confirm the accuracy of the approach. Then, it is subsequently used to characterise the range of existence of instabilities as a function of the Reynolds number, the Mach number, the aspect ratio of the hole and (for the cavity configuration) the dimensionless volume of the cavity [ABSTRACT FROM AUTHOR]

We prove the existence of a global Lipschitz minimizer of functionals of the form \scrI (u) = \int \Omega f(\nabla u(x)) + g(x, u(x)) dx, u \in \phi + W1,1 0 (\Omega), assuming that \phi satisfies the bounded slope condition (BSC). Our assumptions on the Lagrangian allow the function f to be strongly degenerate. [ABSTRACT FROM AUTHOR]

The aim of this paper is to provide a complete description of the bifurcation scenario of a uniform flow past a rotating circular cylinder up to $Re = 200$. Linear stability theory is used to depict the neutral curves and analyse the arising unstable global modes. Three codimension-two bifurcation points are identified, namely a Takens–Bogdanov, a cusp and generalised Hopf, which are closely related to qualitative changes in orbit dynamics. The occurrence of the cusp and Takens–Bogdanov bifurcations for very close parameters (corresponding to an imperfect codimension-three bifurcation) is shown to be responsible for the existence of multiple steady states, as already observed in previous studies. Two bistability regions are identified, the first with two stable fixed points and the second with a fixed point and a cycle. The presence of homoclinic and heteroclinic orbits, which are classical in the presence of Takens–Bogdanov bifurcations, is confirmed by direct numerical simulations. Finally, a weakly nonlinear analysis is performed in the neighbourhood of the generalised Hopf, showing that above this point the Hopf bifurcation is subcritical, leading to a third range of bistability characterised by both a stable fixed point and a stable cycle. [ABSTRACT FROM AUTHOR]

On October 29, 2018, the Vaia storm hits the North-Eastern regions of Italy by wind gusts exceeding 200 km h-1. The forests in these regions have been seriously damaged. This contribution illustrates the methodology adopted in the emergency phase to estimate forest damages caused by Vaia storm, both in terms of damaged forest areas and growing stock volume of fallen trees. 494 Municipalities registered forest damages caused by Vaia, destroyed or intensely damaged forest stands amounted to about 42,500 ha, spread in Trentino Alto Adige, Veneto, Friuli Venezia Giulia, Lombardy and, only marginally, Piedmont and Valle d'Aosta. The growing stock volume of fallen trees was about 8.5 millions m³. [ABSTRACT FROM AUTHOR]

Let E ⊂ ℝ 2 be a closed, bounded set such that E = ⋃ i = 1 k E i , where { E i } i = 1 , ... , k is a family of closed convex sets. We establish an estimate from below of the minimal number of the convex components of E. [ABSTRACT FROM AUTHOR]

On October 29, 2019, the Vaia storm hits the North-Eastern regions of Italy by wind gusts exceeding 200 km h-1. The forests in these regions have been seriously damaged. This contribution illustrates the methodology adopted in the emergency phase to estimate forest damages caused by Vaia storm, both in terms of damaged forest areas and growing stock volume of fallen trees. 494 Municipalities registered forest damages caused by Vaia, destroyed or intensely damaged forest stands amounted to about 42,500 ha, spread in Trentino Alto Adige, Veneto, Friuli Venezia Giulia, Lombardy and, only marginally, Piedmont and Valle d'Aosta. The growing stock volume of fallen trees was about 8.5 millions m³. [ABSTRACT FROM AUTHOR]

The research focused on a collection of 37 Late Antique glass fragments, found at the archaeological site of San Giusto (southern Italy). The analyses performed by SEM-EDS, EMPA and LA-ICP-MS showed that all samples are constituted by natron-based soda-lime silica glass and allowed clarifying the distinction between Egyptian HIMT1 and HIMT2 productions. The comparison with the composition of the available glass reference groups was verified through three ternary diagrams (provided here for the first time). The results grouped San Giusto samples into HIMT1 (7 samples), HIMT2 (8 samples) and Levantine (13, plus maybe other 3 samples) productions. A convincing provenance assignment was not feasible for six samples only. The isotopic analyses (87Sr/86Sr and 143Nd/144Nd), performed on a selected set of ten samples, provided further information both in terms of glass technology and provenance, as well as in terms of reference groups characterisation. Particularly, the results demonstrated that nine out of a total of ten samples were made using a limestone-bearing sand enriched with variable amount of marine shells and provided further evidence for the distinction of the HIMT1 and HIMT2 groups; the latter overlapping the Levantine field in the 87Sr/86Sr versus εNd binary diagram. From a technological point of view, the importance of separating the likely 'fresh' materials from those intentionally recycled or coloured was put into clear evidence, especially in relation to provenance assignments. Lastly, an interesting technological feature has been observed in sample no. 9, where the composition of the main body of the vessel turned out to be different from that of the handle. This result provided clear evidence of the fact that different glass batches could have been used for the different parts of a single vessel body. [ABSTRACT FROM AUTHOR]

The water-cooled lithium-lead breeding blanket is a candidate option for the European Demonstration Power Plant (DEMO) nuclear fusion reactor. This breeding blanket concept relies on the liquid lithium-lead as breeder-multiplier, pressurized water as coolant, and EUROFER as structural material. The current design is based on DEMO 2015 specifications and represents the follow-up of the design developed in 2015. The single-module-segment approach is employed. This is constituted by a basic geometry repeated along the poloidal direction. The power is removed by means of radial-toroidal (i.e., horizontal) water cooling tubes in the breeding zone. The lithium-lead flows in a radial-poloidal direction. On the back of the segment, a 100-mm-thick plate is in charge of withstanding the loads due to normal operation and selected postulated initiating events. Water and lithium-lead manifolds are designed and integrated with a consistent primary heat transport system, based on a reliable pressurized water reactor operating experience, and the lithium-lead system. Rationale and features of the single-module-segment water-cooled lithium-lead breeding blanket design are discussed and supported by thermo-mechanic, thermo-hydraulic, and neutronic analyses. Preliminary integration with the primary heat transfer system, the energy storage system, and the balance of plant is briefly discussed. Open issues, areas of research, and development needs are finally pointed out. @EUROfusion Consortium*, 2017. *For more details see http://www.euro-fusionscipub.org/disclaimer-copyright [ABSTRACT FROM AUTHOR]

A methodology is proposed here to estimate the stability characteristics of bluff-body wakes using local analysis under the assumption of weakly non-parallel flows. In this connection, a generalisation of the classic spatio-temporal stability analysis for fully three-dimensional flows is first described. Secondly, an additional higher-order correction term with respect to the common saddle-point global frequency estimation is included in the analysis. The proposed method is first validated for the case of the flow past a circular cylinder and then applied to two fully three-dimensional flows: the boundary layer flow over a wall-mounted hemispherical body and the wake flow past a fixed sphere. In all the cases considered, both the estimated unstable eigenvalue and the spatial shape of the associated eigenmode are determined by local stability analysis, and they are compared with the reference counterparts obtained at a definitely higher computational cost by a fully three-dimensional global stability analysis. It is shown that the results of local stability analysis, when the higher-order correction term is included, are in excellent agreement with those obtained by global stability analysis. It is also shown that the retained correction term is of crucial importance in this perspective, leading to a remarkable improvement in accuracy with respect to the classical saddle-point estimation. [ABSTRACT FROM AUTHOR]

We study the flow past a sphere rotating in the transverse direction with respect to the incoming uniform flow, and particularly consider the stability features of the wake as a function of the Reynolds number Re and the sphere dimensionless rotation rate Ω. Direct numerical simulations and three-dimensional global stability analyses are performed in the ranges 150 ≤ Re ≤ 300 and 0 ≤ Ω ≤ 1.2. We first describe the base flow, computed as the steady solution of the Navier-Stokes equation, with special attention to the structure of the recirculating region and to the lift force exerted on the sphere. The stability analysis of this base flow shows the existence of two different unstable modes, which occur in different regions of the Re/Ω parameter plane. Mode I, which exists for weak rotations (<0:4), is similar to the unsteady mode existing for a non-rotating sphere. Mode II, which exists for larger rotations (> 0.7), is characterized by a larger frequency. Both modes preserve the planar symmetry of the base flow. We detail the structure of these eigenmodes, as well as their structural sensitivity, using adjoint methods. Considering small rotations, we then compare the numerical results with those obtained using weakly nonlinear approaches. We show that the steady bifurcation occurring for Re>212 for a non-rotating sphere is changed into an imperfect bifurcation, unveiling the existence of two other base-flow solutions which are always unstable. [ABSTRACT FROM AUTHOR]

In the framework of the archaeometric research on Apulian Late Antique glass, a collection of 24 glass vessels (third to seventh centuries ad) and production indicators found at Herdonia (Foggia, Italy) was examined, by means of SEM-EDS, EMPA, ICP-MS and LA-ICP-MS. A fragment of crucible was further investigated by SEM-EDS and EMPA. The results obtained were discussed together with another glass collection from Herdonia previously investigated. The full set of 48 samples was made of siliceous sands mixed with plant ashes (two samples: a dark green slab and a colourless beaker/lamp) or natron (all the other 46 samples). The latter were assigned to the following compositional groups: HIMT, Levantine 1, RNCBGY1, RNCBGY2, RC/LAC-Sb, intermediate HIMT/RNCBGY1, intermediate RNCBGY1/HIMT, intermediate Levantine/RNCBGY2 and two other groups, the CaO-rich HIMT and the CaO-rich/Na2O poor HIMT, which showed a prevailing Adriatic distribution and a close similarity to the weak HIMT glass group. Most Herdonia samples were similar to the HIMT productions, and thus of likely Egyptian origin, while a smaller number of samples referred to the Levantine productions. [ABSTRACT FROM AUTHOR]

Thirty-two glass objects (third to ninth centuries ad) have been investigated by means of SEM-EDS, EMPA, ICP-MS and LA-ICP-MS. Siliceous sands and natron were identified as the vitrifying and the fluxing agents, respectively. Colouring agents were iron for yellow/-green, aqua blue and blackish glass; cobalt for blue and, combined with iron, for brownish glass; and copper and lead antimonates for one emerald green glass. Antimony was the main decolourizer, while manganese was rarely used or effective. Half of the collection was probably of Levantine origin, while the other half was assigned to the North African coast (Egypt?). The reliability of the CaO-rich HIMT glass group has been consolidated. [ABSTRACT FROM AUTHOR]

In this study estimation of tree damage from a windthrow event using feature detection on RGB high resolution imagery is assessed. An accurate quantitative assessment of the damage in terms of volume is important and can be done by ground sampling, which is notably e×pensive and time-consuming, or by manual interpretation and analyses of aerial images. This latter manual method also requires an e×pert operator investing time to manually detect damaged trees and apply relation functions between measures and volume which are also error-prone. In the proposed method RGB images with 0.2 m ground sample distance are analysed using an adaptive template matching method. Ten images corresponding to ten separate study areas are tested. A 13×13 pi×els kernel with a simplified linear-feature representation of a cylinder is applied at different rotation angles (from 0° to 170° at 10° steps). The higher values of the normalized cross-correlation (NCC) of all angles are recorded for each pi×el for each image. Several features are tested: percentiles (75, 80, 85, 90, 95, 99, ma×) and sum and number of pi×els with NCC above 0.55. Three regression methods are tested, multiple regression (mr), support vector machines (svm) with linear kernel and random forests. The first two methods gave the best results. The ground-truth was acquired by ground sampling, and total volumes of damaged trees are estimated for each of the 10 areas. Damaged volumes in the ten areas range from ∼1.8 ×102 m3 to ∼1.2×104 m3. Regression results show that smv regression method over the sum gives an R-squared of 0.92, a mean of absolute errors (MAE) of 255 m3 and a relative absolute error (RAE) of 34% using leave-one-out cross validation from the 10 observations. These initial results are encouraging and support further investigations on more finely tuned kernel template metrics to define an unsupervised image analysis process to automatically assess forest damage from windthrow. [ABSTRACT FROM AUTHOR]

The latest versions of RELAP5-3D© code allow the simulation of thermodynamic system, using different type of working fluids, that is, liquid metals, molten salt, diathermic oil, and so forth, thanks to the ATHENA code integration. The RELAP5-3D© water thermophysical properties are largely verified and validated; however there are not so many experiments to generate the liquid metals ones in particular for the Lead and the Lead Bismuth Eutectic. Recently, new and more accurate experimental data are available for liquid metals. The comparison between these state-of-the-art data and the RELAP5-3D© default thermophysical properties shows some discrepancy; therefore a tool for the generation of new properties binary files has been developed. All the available data came from experiments performed at atmospheric pressure. Therefore, to extend the pressure domain below and above this pressure, the tool fits a semiempirical model (soft sphere model with inverse-power-law potential), specific for the liquid metals. New binary files of thermophysical properties, with a detailed mesh grid of point to reduce the code mass error (especially for the Lead), were generated with this tool. Finally, calculations using a simple natural circulation loop were performed to understand the differences between the default and the new properties. [ABSTRACT FROM AUTHOR]

We study the full three-dimensional instability mechanism past a hemispherical roughness element immersed in a laminar Blasius boundary layer. The inherent threedimensional flow pattern beyond the Hopf bifurcation is characterized by coherent vortical structures usually called hairpin vortices. Direct numerical simulation results are used to analyze the formation and the shedding of hairpin vortices inside the shear layer. The first bifurcation is investigated by global-stability tools. We show the spatial structure of the linear direct and adjoint global eigenmodes of the linearized Navier-Stokes equations and use the structural-sensitivity field to locate the region where the instability mechanism acts. The core of this instability is found to be symmetric and spatially localized in the region immediately downstream of the roughness element. The effect of the variation of the ratio between the obstacle height k and the boundary layer thickness δ* k is also considered. The resulting bifurcation scenario is found to agree well with previous experimental investigations. A limit regime for k/δ*k < 1.5 is attained where the critical Reynolds number is almost constant, Rek ≈ 580. This result indicates that, in these conditions, the only important parameter identifying the bifurcation is the unperturbed (i.e., without the roughness element) velocity slope at the wall. [ABSTRACT FROM AUTHOR]

In this paper we describe a general and systematic approach to the centre-manifold reduction and normal form computation of flows undergoing complicated bifurcations. The proposed algorithm is based on the theoretical work of Coullet & Spiegel (SIAM J. Appl. Maths, vol. 43(4), 1983, pp. 776–821) and can be used to approximate centre manifolds of arbitrary dimension for large-scale dynamical systems depending on a scalar parameter. Compared with the classical multiple-scale technique frequently employed in hydrodynamic stability, the proposed method can be coded in a rather general way without any need to resort to the introduction and tuning of additional time scales. The method is applied to the dynamical system described by the incompressible Navier–Stokes equations showing that high-order, weakly nonlinear models of bifurcating flows can be derived automatically, even for multiple codimension bifurcations. We first validate the method on the primary Hopf bifurcation of the flow past a circular cylinder and after we illustrate its application to a codimension-two bifurcation arising in the flow past two side-by-side circular cylinders. [ABSTRACT FROM PUBLISHER]

Sensitivity analysis has successfully located the most efficient regions in which to apply passive control in many globally unstable flows. As is shown here and in previous studies, the standard sensitivity analysis, which is linear (first order) with respect to the actuation amplitude, predicts that steady spanwise wavy alternating actuation/modification has no effect on the stability of planar flows, because the eigenvalue change integrates to zero in the spanwise direction. In experiments, however, spanwise wavy modification has been shown to stabilize the flow behind a cylinder quite efficiently. In this paper, we generalize sensitivity analysis by examining the eigenvalue drift (including stabilization/destabilization) up to second order in the perturbation, and show how the second-order eigenvalue changes can be computed numerically by overlapping the adjoint eigenfunction with the first-order global eigenmode correction, shown here for the first time. We confirm the prediction against a direct computation, showing that the eigenvalue drift due to a spanwise wavy base flow modification is of second order. Further analysis reveals that the second-order change in the eigenvalue arises through a resonance of the original (2-D) eigenmode with other unperturbed eigenmodes that have the same spanwise wavelength as the base flow modification. The eigenvalue drift due to each mode interaction is inversely proportional to the distance between the eigenvalues of the modes (which is similar to resonance), but also depends on mutual overlap of direct and adjoint eigenfunctions (which is similar to pseudoresonance). By this argument, and by calculating the most sensitive regions identified by our analysis, we explain why an in-phase actuation/modification is better than an out-of-phase actuation for control of wake flows by spanwise wavy suction and blowing. We also explain why wavelengths several times longer than the wake thickness are more efficient than short wavelengths. [ABSTRACT FROM PUBLISHER]

The design and development of high-speed data wireless communication systems within buildings call for the investigation of the ultrawideband indoor propagation channel. To date, ray tracing (RT) based approaches have been widely used to characterise the channel; unfortunately, such techniques, although accurate, turn out computationally intensive. In this work, a parallel ray (PR) approximation is successfully used to significantly improve the computational efficiency of the RT method. The accuracy and the applicability of the proposed approximation are assessed through the analysis of the mean square error between the impulse responses obtained with the PR and the RT methods. [ABSTRACT FROM AUTHOR]

An efficient and flexible air interface is the necessary condition to achieve the ambitious goals of next generation broadband wireless networks. Multicarrier code division multiple access (MC-CDMA) is a practical answer to this need, but the considerable vulnerability to nonlinear distortions induced by high power amplifiers at the transmitter side hinders its appealing features, thereby demanding for adequate countermeasures. The current paper contributes to this issue with a novel multiuser data predistortion (MUDP) procedure aimed at mitigating the performance degradation of a MC-CDMA forward-link in the presence of a nonlinear channel. The proposed MUDP resides entirely at the transmitter of the base station, and simply requires a set of modified constellations, which are jointly and iteratively optimized so that the distortions experienced by each active user are significantly reduced. The required computational complexity is then kept at affordable levels by resorting to a suboptimal yet effective scheme based on the identification of the strongest interfering users combined with the set-partitioning and the symmetry properties of their constellations. Numerical simulation results corroborate the effectiveness of the MUDP in improving the link performance under typical demanding multiple-access operating conditions. [ABSTRACT FROM PUBLISHER]

In this article, the accuracy estimation of radio location techniques throughout ray-tracing-based propagation models has been discussed. Assuming as a reference scenario a 16-building Manhattan environment, some numerical results for 2-D location accuracy of some location techniques have been presented and discussed. It has clearly been shown that a site-specific simulation tool can easily account for NLOS conditions and multipath effects. Moreover, other error sources, such as thermal noise and measurement uncertainties, can be introduced if their statistics are known a priori. Therefore, validation and improvement of existing mobile location and tracking techniques, as well as the deployment of new and more accurate algorithms, can resort to deterministic propagation tools as a viable alternative to expensive field trials or approximate stochastic models. [ABSTRACT FROM PUBLISHER]

This correspondence presents the architecture of a high-speed low-complexity digital receiver for wideband satellite communications supporting multiple baud rates, ranging from 10 to 200 MBaud. The design of the modem is based on the Cascaded Integrator-Comb (CIC) scheme which exploits a parallel processing so as to provide an outstanding decimation agility. Eventually, bit error rate (BER) performance of the multi-rate receiver are assessed by floating-point simulations. [ABSTRACT FROM AUTHOR]

Since 1999, ESA has conducted a number of system studies and technological developments to support the European and Canadian industry in the definition of a Satellite UMTS (S-UMTS) component development strategy, identifying critical technological areas and promoting S-UMTS demonstrations. In particular, the MUSIC project has successfully demonstrated advanced digital signal processing techniques to mitigate CDMA interference in spread-spectrum receivers, thus increasing the capacity and/or quality of service of a wireless communication network. The low-complexity solution for interference mitigation investigated and developed in the project is particularly suited for being implemented in mobile terminals. [ABSTRACT FROM PUBLISHER]