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The physicochemical and biological characteristics of milli-microbubbles were compared to evaluate their performance on hydrogenotrophic denitrification (HD) for groundwater treatment in remote areas. The hydrogen supply was controlled at 1.14 L/d with 40 mgN/L of NO3-N. The microbial community structure in two bubble reactors was investigated by high throughput sequencing. Microbubbles enhanced biodegradation in the HD system, providing a maximum nitrogen removal efficiency of 99%. Approximately 50% of total hydrogen was utilized for biological nitrate removal with the highest hydrogen effectiveness achieved at 1.21 g N/g H2. In contrast, millibubbles achieved less than 10% efficiency and 9.9% of total hydrogen was consumed for biological nitrogen removal. Thauera spp., Hydrogenophaga spp. and Rhodocyclaceae of Proteobacteria phylum were the dominant bacteria in the microbubble reactor, whereas Methyloversatilis spp. was dominant in the millibubble reactor, in which a relatively low amount of hydrogen (0.6 mg/L) was dissolved. The differences can be attributed to the higher hydrogen transfer efficiency (45x10-3 s-1) and lower rising velocity (0.31 mm/s) of the microbubbles system than the millibubbles system (2x10-3 s-1 and 480 mm/s). The micro-hydrogen bubble technology affords increased hydrogen effectiveness, reduced energy consumption, and modified system design. Therefore, it is more appropriate for enhancing HD. [ABSTRACT FROM AUTHOR]

Nitrate-contamination in groundwater sources has been globally taken as a crucial issue due to the effects of nitrate on human health, e.g., methaemoglobinaemia. Various processes have been applied for the treatment of contaminated groundwater with nitrate. Hydrogen-based autotrophic denitrification (hydrogenotrophic denitrification) is an alternative process used for abatement of the nitrate-contamination. Although high efficiency on nitrate removal was achieved by former research, it is unclear whether the growth of true denitrifiers is affected by inorganic carbon in which this is essential to comprehend the principal cause of nitrite accumulation. This study found that inorganic carbon had no impact on the nitrate reduction rate whereas the nitrite reduction rate was improved when a higher amount of inorganic carbon was supplied. Also, using bicarbonate as an inorganic carbon source had a positive effect on the growth and adaptation of true denitrifiers that can reduce the nitrite accumulation in hydrogen-based denitrification system. [ABSTRACT FROM AUTHOR]

Nitrate removal during anaerobic ammonium oxidation (anammox) treatment is a concern for optimization of the anammox process. This study demonstrated the applicability and long-term stability of the coupled anammox and hydrogenotrophic denitrification (CAHD) process as an alternative method for nitrate removal. Laboratory-scale fixed bed anammox reactors (FBR) supplied with H2 to support denitrification were operated under two types of synthetic water. The FBRs showed simultaneous NH4-N and NO3-N removal, indicating that the CAHD process can support NO3- N removal during the anammox process. Intermittent H2 supply (e.g. 5 mL/min for a 1-L reactor, 14/6-min on/off cycle) helped maintain the CAHD process without deteriorating its performance under long-term operation and resulted in a nitrogen removal rate of 0.21 kg-N/m³/d and ammonium, nitrate, and dissolved inorganic nitrogen removal efficiencies of 73.4%, 80.4%, and 77%, respectively. The microbial community structure related to the CAHD process was not influenced by changes in influent water quality, and included the anammox bacteria 'Candidatus Jettenia' and a Sulfuritalea hydrogenivorans-like species as the dominant bacteria even after long-term reactor operation, suggesting that these bacteria are key to the CAHD process. These results indicate that the CAHD process is a promising method for enhancing the efficiency of anammox process. [ABSTRACT FROM AUTHOR]

This study investigates the contribution ratios of different groundwater recharge sources and influence of a dual monsoon system in Kofu basin, central Japan, through the hydrogen and oxygen isotopic analysis of precipitation, river water, and groundwater. The study is focused on the area of the Kamanashigawa and Midaigawa alluvial fans, which are formed by two main rivers. Precipitation isotopic content exhibits significant seasonal variability. Also, river water presents d-excess values higher than annual precipitation at plain areas (9 and 10‰), suggesting that two different air-masses contribute to precipitation, corresponding to the monsoon and pre-monsoon periods. The results of this study allow estimation of relative contributions of different sources to groundwater and influence of a dual monsoon system. The mass-balance analysis of the d18O to identify the groundwater source indicates the river water contributes 38-100% of the recharge, while precipitation contributes 29-62% in Kamanashigawa alluvial fan. In the case of Midaigawa alluvial fan, river water contributes 77-99% in the northern part; in the southern side, 30-93% of contribution comes from precipitation. The mass-balance analysis of the d-excess indicates pre-monsoon precipitation contributes 46-68% and 39-65% to groundwater of the Kamanashigawa and Midaigawa alluvial fans, respectively. [ABSTRACT FROM AUTHOR]

Information regarding domestic water consumption is vital, as the Kathmandu Valley will soon be implementing the Melamchi Water Supply Project; however, updated information on the current situation after the 2015 Gorkha Earthquake (GEQ) is still lacking. We investigated the dynamics of domestic water consumption pre- and post-GEQ. The piped water supply was short, and consumption varied widely across the Kathmandu Upatyaka Khanepani Limited (KUKL) branches and altitude. The reduction in piped, ground, and jar water consumption and the increase in tanker water consumption post-GEQ appeared to be due to the impact of the GEQ. However, the impact did not appear to be prominent on per capita water consumption, although it was reduced from 117 to 99 L post-GEQ. Piped, ground, and tanker water use were associated with an increase and jar water use was associated with a decrease in water consumption. Despite improvements in quantity, inequality in water consumption and inequity in affordability across wealth status was well established. This study suggests to KUKL the areas of priority where improvements to supply are required, and recommends an emphasis on resuming performance. Policy planners should consider the existing inequity in affordability, which is a major issue in the United Nations Sustainable Development Goals. [ABSTRACT FROM AUTHOR]

The distribution of benthic macroinvertebrates was studied in deep Lake Motosu (oligotrophic lake), Japan. Bathymetrical sampling surveys were carried out using a standard Ekman grab at each of 16 stations in the lake on 8 March 2013. The average densities of the benthic community for all the stations were 2185 ind./m2, comprised principally of Tubifex tubifex (Müller) 23.4%, Asellus hilgendorfi Bovallius 4.9% and chironomids 65.8%. T. tubifex and chironomids inhabited the entire lake bottom, with higher densities in the shallower region (25 m>) than in the deeper region (ca. 120 m). On the other hand, the density of A. hilgendorfi increased with water depth and was highest in the deeper regions (>70 m). The densities of chironomid larvae were lower in the deeper region, with fewer chironomid larvae located in the center of the lake (637 ind./m2). The most abundant species, Micropsectra yunoprima Sasa, exhibited the widest distribution from 10.0 m to 121.0 m (mean density 926 ind./m2), followed by Procladius choreusi (Meigen) from 10 m to 74.1 m (mean density 365 ind./m2). The results of this study were compared to previous data by Kitagawa (1973) on chironomid larvae. The densities of these chironomid larvae increased throughout the whole lake in 2013. The water quality has not undergone a major change since the 1970's. In this study, the number of M. yunoprima larvae has shown a tendency to increase, especially in shallower regions; where they are widely distributed, suggesting the continuation of favorable bottom condition for larvae since the 1970's. [ABSTRACT FROM AUTHOR]

This study estimated spatial and seasonal variation of water quality to understand characteristics of Nakdong river basin, Korea. All together 11 parameters (discharge, water temperature, dissolved oxygen, 5-day biochemical oxygen demand, chemical oxygen demand, pH, suspended solids, electrical conductivity, total nitrogen, total phosphorus, and total organic carbon) at 22 different sites for the period of 2003-2011 were analyzed using multivariate statistical techniques (cluster analysis, principal component analysis and factor analysis). Hierarchical cluster analysis grouped whole river basin into three zones, i.e., relatively less polluted (LP), medium polluted (MP) and highly polluted (HP) based on similarity of water quality characteristics. The results of factor analysis/principal component analysis explained up to 83.0%, 81.7% and 82.7% of total variance in water quality data of LP, MP, and HP zones, respectively. The rotated components of PCA obtained from factor analysis indicate that the parameters responsible for water quality variations were mainly related to discharge and total pollution loads (non-point pollution source) in LP, MP and HP areas; organic and nutrient pollution in LP and HP zones; and temperature, DO and TN in LP zone. This study demonstrates the usefulness of multivariate statistical techniques for analysis and interpretation of multi-parameter, multi-location and multi-year data sets. [ABSTRACT FROM AUTHOR]

In order to assess the stability of nitrogen removal systems utilizing anaerobic ammonium oxidation (anammox), it is necessary to study the toxic effects of nitrite on these biochemical reactions. In this study, the effects of nitrite on anammox bacteria entrapped in gel carriers were investigated using batch and continuous feeding tests. The results showed that the nitrite concentration in a reactor must be less than 274-mg N/L in order to prevent a decrease in the anammox activity, which occurred when the gel carriers were soaked in nitrite solutions with concentrations greater than 274-mg N/L in a batch test. In a continuous feeding test, nitrite inhibition was not observed at low concentrations of nitrite. However, the anammox activity decreased to 10% when the nitrite concentration increased to 750-mg N/L over a 7-day period in the reactor. In addition, it was shown that the effects of nitrogen on the anammox reaction were reversible because the anammox activity completely recovered within 3 days when the influent nitrite concentration was decreased to less than 274-mg N/L. [ABSTRACT FROM AUTHOR]

Abstract  Empirical data that describe the metabolic balance of stream ecosystems in human-dominated watersheds are scarce. We measured ecosystem metabolism in 23 open-canopied lowland streams draining urban and agricultural areas in the Fuji River Basin, central Japan. Gross primary production (GPP) and community respiration (CR) were estimated using the diurnal dissolved oxygen (DO) change technique, with the reaeration coefficient (K 2) determined from seven empirical depth-velocity equations. Because the predicted values of K 2 showed variation among the depth-velocity equations, the estimates of stream metabolism also varied according to the equations. However, CR was almost always greater than GPP, resulting in negative net ecosystem production (NEP) and GPP/CR ratios below unity for most of the study reaches. Highly heterotrophic streams were found in intensively farmed watersheds, suggesting that organic matter loading from agricultural lands is likely to be a source of allochthonous carbon fueling excess respiration in the study streams. In contrast, streams draining more urbanized areas were less heterotrophic. The present results suggest that lowland streams in agriculturally developed watersheds are associated strongly with terrestrial ecosystems as a source of organic carbon. The resultant strong respiration might become the dominant process in ecosystem metabolism, as reported for headwater streams, large downstream rivers, and estuaries. [ABSTRACT FROM AUTHOR]

Benthic macroinvertebrate distribution was studied on April 9, 1994, and March 2, 2003, in Lake Yamanakako. The average density of the benthic community for the entire lake was 3168?ind.?m -2, comprising principally oligochaetes (41.0%) and chironomids (59.0%) in 1994. In 2003, the benthic community for the entire lake was 1847?ind.?m -2, principally consisting of oligochaetes (69.9%) and chironomids (30.1%). In 1994, the larval density of Propsilocerus akamusi was 3.5 times that of Chironomus nipponensis and in 2003 the figure was 5.7 times. However, the larval biomass of P. akamusi was 2.1 times greater than that of C. nipponensis in 1994 and 2.8 times greater in 2003. The larval density of Tanypodinae decreased drastically, by about 12-fold, from 1994 to 2003. P. akamusi larvae were particularly abundant at the lake center in 1994, but they inhabited the entire lake bottom in 2003. P. akamusi density was closely related to water depth and ignition loss. C. nipponensis larvae also showed the widest distribution pattern in 2003, whereas their larvae had inhabited the northeastern parts and the lake center in 1994. Recently, the number of C. nipponensis larvae in Lake Yamanakako is tending to decrease, whereas that of P. akamusi larvae is increasing, suggesting ongoing eutrophication. [ABSTRACT FROM AUTHOR]

This study analyses and evaluates the impact of two recent (2001 and 2010) education reforms on Ukrainian secondary schools, and, in particular, on science subjects (physics, chemistry, biology and Earth science), in terms of the structural, quantitative and qualitative characteristics of the reformed disciplines. It also examines societal attitudes towards the changes introduced in education and explains the reasons behind them. The 2001 reform prescribed a gradual shift from a 10- or 11-year school system to a 12-year one as a measure needed for the integration of Ukraine into the European Higher Education Area according to the Bologna Process. The first graduates of the new course of study would have completed it in 2013. However, the reform implementation was interrupted on 6 July 2010, when the newly elected president signed Law 2442‑17, which became a starting point for the 2010 reform enacting the return to 11 years of study. Both reforms were politically charged and met with a mainly negative attitude by the general population, which was particularly evident in the responses of teachers who considered certain key aspects of the 2001 and 2010 reforms to be an unnecessary political interference in an otherwise effective model of schooling. [ABSTRACT FROM AUTHOR]