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Copyright of Revista Brasileira de Engenharia Agricola e Ambiental - Agriambi is the property of Revista Brasileira de Engenharia Agricola e Ambiental and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background/Objectives: Crude extracts from the Brassica genus have recently emerged as promising phytochemicals for preventing bone loss. While the most documented evidence suggests that their general biological activity is due to glucosinolates' (GLSs') hydrolysis products, the direct activity of GLSs is beginning to be uncovered. However, the contribution of GLSs to the bone-sparing activity of crude Brassicaceae extracts has seldom been addressed. Here, we aimed to gain insights into this gap by studying in the same in vitro model of human osteogenesis the effect of two Brassica seed extracts (Eruca sativa and Lepidium sativum) obtained from defatted seed meals, comparing them to the isolated GLSs most represented in their composition, glucoerucin (GER) and glucotropaeolin (GTL), for Eruca sativa and Lepidium sativum, respectively. Methods: Osteogenic differentiation of human mesenchymal stromal cells (hMSCs) was assessed by alizarin red staining assay and real-time PCR, respectively, evaluating mineral apposition and mRNA expression of specific osteogenic genes. Results: Both Brassica extracts and GLSs increased the osteogenic differentiation, indicating that the stimulating effect of Brassica extracts can be at least partially attributed to GLSs. Moreover, these data extend previous evidence of the effect of unhydrolyzed glucoraphanin (GRA) on osteogenesis to other types of GLSs: GER and GTL. Notably, E. sativa extract and GTL induced higher osteogenic stimulation than Lepidium sativum extract and GER, respectively. Conclusions: Overall, this study expands the knowledge on the possible application of Brassica-derived bioactive molecules as natural alternatives for the prevention and treatment of bone-loss pathologies. [ABSTRACT FROM AUTHOR]

The effects of liquid organic fertilizer (LOF) made from anaerobic digestion on some physiological factors and volatile components of arugula plants that were grown in salty conditions were investigated. The experiment was conducted in three different stages. In the first two stages, seeds were grown in petri dishes and pots for seven days. The third stage involved growing seedlings in pots for 60 days. Salinity inhibited the germination of 7-day arugula seedlings in petri dishes and their emergence in pots. In these stages, LOF pretreatment failed to eliminate stress-induced inhibition. Some physiological parameters were analyzed in 60-day seedlings in the third stage. Salt-induced inhibition showed significant negative effects on all parameters. In contrast to previous stages, LOF (1% and 5%) and NPK had positive effects in all groups at the third stage, eliminating salt stress in all parameters except water content. Contrary to expectations, volatile components showed no significant change but had fluctuating values due to salt stress or fertilizer treatments. [ABSTRACT FROM AUTHOR]

This study showed the eliciting effect of aqueous extracts from Ascophyllum nodosum on the development of Eruca sativa microgreens in a quartz-based substrate. There is no information in the literature on the impact of its use on the quality, bioactive compound content, and nutritional value of arugula microgreens. Assays that have been made include the following: total phenolic content (TPC), total flavonoid content (TFV), enzymes such as phenylalanine ammonia (PAL) and polyphenol oxidase (PPO), and enzymes involved in the scavenging of reactive oxygen species such as catalase (CAT) and superoxide dismutase (SOD). The antioxidant activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) was also evaluated. The total phenolic content of arugula microgreens increased significantly after an application of aqueous extracts of A. nodosum to the substrate. The greatest increase in flavonoid content (89%) and antioxidant activity against DPPH (82%) was observed at a concentration of 2.5%. The highest increase in activity of CAT (68.2%), SOD (25%), PAL (13-fold), and PPO (84.2%) was observed with the application of 5% extract. In conclusion, the use of Ascophyllum nodosum algae affects TPC and TFV, antioxidant activity, PAL, PPO, SOD, and CAT in the microgreens studied. By prioritising organic and environmentally friendly extracts, growers can contribute to a more sustainable and healthier food system, especially in the case of the production of Eruca sativa microgreens. [ABSTRACT FROM AUTHOR]

BACKGROUND: Aquaponic systems are sustainable processes of managing water and nutrients for food production. An innovate nutrient‐efficient catfish‐based (Clarias gariepinus) aquaponics system was implemented for producing two cultivars of two leafy vegetables largely consumed worldwide: lamb's lettuce (Valerianella locusta var. Favor and Valerianella locusta var. de Hollande) and arugula (Eruca vesicaria var. sativa and Eruca sativa). Different growing treatments (4 × 2 factorial design) were applied to plants of each cultivar, grown at two light intensities (120 and 400 μmol m−2 s−1). During growth, several morphological characteristics (root length, plant height, leaf number, foliage diameter and biggest leaf length) were measured. At harvest, plants were weighed and examined qualitatively in terms of greenness and health status. Additionally, leaf extracts were obtained and used to determine total phenolic contents, antioxidant capacities, and levels of cytotoxicity to Caco‐2 intestinal model cells. RESULTS: After a 5‐week growth period, both lamb's lettuce cultivars presented high levels of greenness and health status, at both light intensities, particularly the var. de Hollande that also showed higher average performance in terms of plant morphology. In turn, arugula cultivars showed lower levels of greenness and health status, especially the cultivar E. vesicaria var. sativa submitted to direct sunlight during growth. In addition, plant specimens submitted to higher levels of light intensity showed higher contents in antioxidants/polyphenols. Cultivars with a higher content in antioxidants/polyphenols led to higher Caco‐2 cell viability. CONCLUSION: For successful industrial implementation of the aquaponics technology, different and optimized acclimatizing conditions must be applied to different plant species and cultivars. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

There is a demand from the scientific, beekeeping and consumer sectors to characterize honey based on its botanical origin, as it provides unique and distinctive properties. Nevertheless, existing studies on the physicochemical properties and the sensorial profile of honey in relation to botanical origin remain insufficient. This study aimed to understand the relationships between sensory profile and various chemical compounds (minerals, sugars, water content and volatiles) of three monofloral honeys (Atractylis serratuloides, Retama sphaerocarpa and Eruca sativa) produced in Algeria using principal component analysis. Crystallization was detected as a distinctive attribute of Eruca and Atractylis honey. A candy aroma and odor with floral nuances, light color, crystallized state and the volatile compounds Alpha-Bisabolol and Beta-eudesmol characterized the Atractylis honey. Eruca honeys were distinguished by an animal and degraded odor, bitter taste, light color and the presence of Dimethyl trisulfide and Dimethyl tetrasulfide. Finally, a vegetal aroma, some saltiness and sourness, dark amber color, lower sugar content, higher K content and Lilac aldehyde and Lilac aldehyde D characterized Retama honeys. [ABSTRACT FROM AUTHOR]

Copyright of Revista Verde de Agroecologia e Desenvolvimento Sustentável is the property of Revista Verde de Agroecologia e Desenvolvimento Sustentavel and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The current study aimed to determine the polyphenol compounds in Nigella sativa (NS) and Eruca sativa (ES) seeds, and evaluate the impact of their addition either as a sole additive or in combination on the growth performance, digestibility, some rumen and blood parameters and antioxidative status of Barki lambs. Forty-eight male lambs (27.18 ± 0.22 kg, 5–6 months), were divided into 4 balanced groups. The experimental diets were randomly distributed to the control group (CON); fed alfalfa hay plus concentrate feed mixture at a ratio of 30:70% without additives, while, NSD, ESD, and NESD groups: fed CON diet plus 2% NS, 2% ES or 1% NS + 1% ES, respectively as a ratio from total mixed ration (TMR). Results indicated that rutin and catechin were the most phenolic compounds observed either in NS or ES seeds. The NS and ES-supplemented groups recorded the highest (P < 0.05) values for dry matter digestibility, nutritive values, average daily gain, and the best feed conversion ratio. However, growth performance, nutritive value, and all nutrient digestibility except for dry matter were not significantly altered with the NESD group. Concentrations of ruminal NH3-N and TVFA were significantly (P < 0.05) reduced with the NESD group, with no significant differences in pH values among different groups. Values of blood parameters showed significant increases in WBCs, PCV, and T-AOC, and decreases in cholesterol, triglycerides, and MDA with the addition of NS and ES seeds or both. Therefore, the addition of NS and ES seeds is recommended to improve lambs’ health and antioxidant status. [ABSTRACT FROM AUTHOR]

A growing interest has been drawn to the use of traditional medicinal plants for the treatment of human diseases and, in particular, infertility and reproductive toxicity associated with environmental factors. The Mediterranean basin area is a recognized source of plant species with therapeutic interest. In this frame, Eruca sativa (ES) is an annual edible plant and a member of the Brassicaceae family. A relatively large number of studies, focusing on the biological effects of the extract from the leaves of ES on in vitro and in vivo models of disease, have been published in recent years. The present narrative review aims to analyze the phytochemical constituents, traditional uses, possible pharmacological activities, and recognized effects of ES on male reproductive outcomes. Available investigations have revealed the presence of a number of compounds with antioxidant properties, such as polyphenols, glucosinolates, flavonoids, and carotenoids in extracts from ES. Based on the chemical and pharmacological characteristics of the aforementioned compounds, we show that ES has possible preventive properties and therapeutic uses, especially in the functional derangements of the male reproductive system. [ABSTRACT FROM AUTHOR]

Arugula is a leafy vegetable that has been standing out in terms of consumption due to its nutritional content. The crop has low tolerance to water salinity (ECw), and information on the management of fertilization in saline environments is scarce. In this context, this study aimed to analyze the nutritional aspects of arugula under different soil salinity levels and fertilization doses via fertigation. The experiment was conducted in a greenhouse during two production cycles in 2021 in a randomized block design and a 4 x 3 factorial arrangement, with four replications. The treatments consisted of four soil salinity levels (ECse = 0.57, 1.3, 2.3, and 3.3 dS m-1) and three doses of a mineral fertilizer based on the recommended fertilization via fertigation (F1 = 100%, F2 = 50%, and F3 = 25%). The contents of N, P, K, Ca, Mg, S, Na, Cu, Fe, Zn, and Mn were evaluated after harvesting. Salinity directly affected the concentration of mineral elements in arugula leaf during the first cycle, mainly Ca, Mg, Zn, and Mn. The fertigation strategy with 100% of the recommended fertilization showed better results than the other doses for N, P, K, and S. In the second cycle, all elements showed a reduction due to the high ECse, mainly the value of 3.3 dS m-1. The fertigation strategy that used 100% of the recommended fertilization promoted higher nutrient accumulation in arugula. The order of nutrient accumulation in arugula leaf was K>N>Ca>S>Mg>P>Fe>Zn>Mn>Cu. [ABSTRACT FROM AUTHOR]

Copyright of Agricultural Research in the Tropics / Pesquisa Agropecuária Tropical is the property of Pesquisa Agropecuaria Tropical and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Agricultural Research in the Tropics / Pesquisa Agropecuária Tropical is the property of Pesquisa Agropecuaria Tropical and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Egyptian Journal of Histology is the property of Egyptian Journal of Histology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Spaceflight is known to produce genetic changes in seeds, usually accelerating aging, though species and varietal differences have been poorly investigated. Comparisons were carried out in terms of yield, biochemical characteristics and mineral composition between mature plants grown from seeds subjected to a one-year spaceflight, belonging to Brassicaceae (Brassica juncea and Eruca sativa), Apiaceae (Anethus graveolens, and Coriandrum sativum), and Asteraceae (Lactuca sativa, six cultivars) families, and non-treated control. Among the studied species, only Brassica juncea and Eruca sativa demonstrated a growth stimulation effect caused by seed spaceflight, while significant growth inhibition was recorded in Apiaceae plants and three cultivars of Lactuca sativa L. No differences in the total antioxidant activity (AOA), polyphenol and ascorbic acid content were detected between 'space-treated' and control plants. On the contrary, significant decrease in proline accumulation and increase in malonic dialdehyde and photosynthetic pigments levels were shown by Brassicaceae species. The effect of long-term seed spaceflight on the mineral composition of mature plants was reflected in the inhibition of accumulation of all 24 elements analyzed in Apiaceae plants, except for Se, whose concentration was higher in all 'space-treated' plants compared to the control. Spaceflight seed storage increased V levels in lettuce and decreased Na accumulation in all the investigated species. The results reveal species-dependent changes in the accumulation of macro-, micro- and toxic elements in Apiaceae, Brassicacea, and Asteraceae representatives due to spaceflight seed storage. The detected differences in plant elemental composition between 'space' treatment and control partly explain the corresponding yield gap and suggest a relationship between mineral status and adaptability. The highest beneficial effect of spaceflight seed storage on yield was recorded in Eruca sativa cultivar, Rusalochka. [ABSTRACT FROM AUTHOR]

The study was performed to examine the role of heme oxygenase (HO) in tolerance and accumulation of cadmium in Eruca sativa (var. DPY BKP 98). Seedlings of E. sativa were subjected to different levels of CdCl2 (10 to 150 μM) for 96 h. The result shows that lower dose of Cd (10 to 25 μM) had mild effect on plant biomass and photosynthetic pigments due to which E. sativa can efficiently uptake and translocate Cd, but higher concentration significantly reduces these attributes by increasing membrane damage (MDA and H2O2 content) and downregulating the activities of major antioxidants. However, at 125 μM treatment improved HO activity was recorded that enhances plant growth by increasing plant biomass and chlorophyll content; augmented activities of antioxidants (proline, APX, and CAT); lowered oxidative damage; and increased tolerance ability of plant toward Cd. Interestingly over-expression of HO gene at 125 μM increases Cd accretion and bioconcentration factor (BCF) both in leaves and roots, and enhances the translocation of cadmium from roots to leaves. Thus, our findings for the first time proposed that HO operates dual function in Eruca seedlings by rising phytoremediation efficiency and escalating stress tolerance via upregulating antioxidant defense machinery. [ABSTRACT FROM AUTHOR]

The intensive use of pesticides causes harmful effects on ecosystems, and remediation techniques can reduce the ecotoxicity of contaminated soils. Sugarcane straw is a feasible feedstock for biochar production, and it is abundant in the ethanol industry. In this sense, this study assessed the application of biochar from sugarcane straw to reduce the ecotoxicity of soils contaminated with the pesticides fipronil and 2,4-D, alone and in a mixture. Tests at the microcosm level (28 days) evaluated the influence of biochar on the terrestrial worm Enchytraeus crypticus and the eudicot plant Eruca sativa L. At the end of the experiment, elutriate solutions were prepared with soil samples to assess responses on E. sativa and on the cladoceran Daphnia similis. Fipronil and 2,4-D decreased the reproduction of E. crypticus in microcosms, particularly on treatments with fipronil. Biochar reduced the ecotoxicity of fipronil in soils, increasing the reproduction of E. crypticus by 43% compared with soils without biochar. However, the control with biochar showed a reproduction reduction of 23% when compared with the control without biochar. In soils with 2,4-D, the germination rates and shoot growth of E. sativa were 2 times greater in biochar-remediated soils compared with untreated soils. Considering the exposure to elutriate, the application of biochar in soils with 2,4-D improved the growth (by 45%) and germination (by 34%) of E. sativa. No effects were identified on D. similis at any exposure scenario. Therefore, sugarcane straw biochar can be a viable alternative for reducing the ecotoxicity of pesticides in soils. [ABSTRACT FROM AUTHOR]

Copyright of Revista Brasileira de Engenharia Agricola e Ambiental - Agriambi is the property of Revista Brasileira de Engenharia Agricola e Ambiental and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

A field experiment was conducted to explore the possibilities of sustainable crop intensification in the fallow–Eruca sativa Mill. system in arid ecology by replacing fallow with short-duration sorghum–legume intercropping. The experiment was laid out in a split-plot design with two planting systems (bed and conventional) in main plots and a factorial combination of crop duration (50 and 60 days) and cropping systems (sole sorghum, sorghum + cowpea, sorghum + Sesbania in 2:2 ratio) in sub-plots. In the succeeding Eruca sativa crop, residuals and two gypsum levels (0 and 250 kg ha−1) were tested. Bed planting practiced during both seasons did not improve the system productivity to significant levels. Extending the duration of fallow replacement crops from 50 to 60 days significantly increased their forage yield, overall system productivity by 25–34%, and system net returns by 15.9–21.5%. Amongst the intercropping systems, the sorghum + Sesbania system added 10–13 tonnes ha−1Sesbania biomass to the soil, resulting in higher soil organic carbon, available nitrogen, dehydrogenase activity, and residual soil moisture, which increased the yield of the succeeding Eruca sativa crop by 8.8–15% compared to the residual of sole sorghum. However, it could not compensate for the yield loss due to the utilization of 50% of the area for growing the green manure crop. The sorghum + cowpea intercropping–Eruca sativa system was found to be the optimum combination with a system productivity of 1.27–1.87 Mg ha−1Eruca sativa seed equivalent. The productivity of Eruca sativa further improved by 9.5–23.7% due to the soil application of gypsum @ 250 kg ha−1. When averaged over treatments, fallow replacement during the rainy season reduced the available soil moisture at the sowing of Eruca sativa by 8.3–22.8% and subsequently its yield by 16.5–30.4% compared to the fallow–Eruca sativa system. However, with this production penalty, an additional rainy-season fodder crop was successfully grown, which improved the system productivity by 57.7–82.8%, net returns by 31.2–57.3%, and rainfall use efficiency from 0.21 to 36 USD/mm−1 ha−1. Hence, it may be concluded that short-duration fodder crops may be taken as fallow replacement crops for higher system productivity and rainfall use efficiency. [ABSTRACT FROM AUTHOR]

Enzymatic browning reactions by polyphenol oxidases cause alteration of appearance, flavor and nutritive value of vegetables and fruits. It is one of the important problems for vegetables used as salads and causes lots of economic losses. In this study, polyphenol oxidase (PPO) from Eruca sativa was extracted and characterization studies were carried out. Substrate specificity for variable substrates, optimum pH, optimum temperature, effect of different metal ions were assayed. Km and Vmax values were determined as for pyrocatechol Km = 10.24 mM, Vmax = 0.0018 U min-1, catechin; Km = 12.57 mM, Vmax = 0.0012 U min-1, gallic acid; Km = 23.07 mM, Vmax = 0.0001 U min-1 . Optimum pH and temperature were determined as pH:7.0 and 20°C respectively. Effect of various metal ions such as, K+, Fe2+, Mg2+, Zn2+, Cu2+ and Hg2+ on enzyme activity were measured. K+, Mg2+, Zn2+, Cu2+ ions inhibited PPO activity significantly. However Fe2+ ion did not inhibit PPO activity. [ABSTRACT FROM AUTHOR]

The effects of combinations of oxalic acid (OA) treatment with modified atmosphere packaging on the quality and biochemical content changes of rocket (Eruca sativa Mill. cv. Bengi) leaves were examined. After harvest, selected leaves were dipped into an aqueous solution containing different concentrations of oxalic acid (0-control, 0.25 mM, 0.5 mM, and 1 mM) for 1 min. Treated samples were dried and placed in modified atmosphere packages. Treated rockets were stored at two different temperatures (0 °C and 10 °C) and 90 ± 5% relative humidity conditions for 10 days. Leaves were analyzed at 2-day intervals for some quality and biochemical parameters during storage. OA-treated leaves were greener than those of the control group. At the end of the storage, high doses (1 mM) of OA applications successfully suppressed the respiration rate (0 °C: 63.12 mL CO2 kg−1 h−1, 10 °C: 78.09 mL CO2 kg−1 h−1) and retarded the weight loss (0 °C: 0.14%, 10 °C: 0.49%) and color discoloration (0 °C: ∆E 7.23, 10 °C: ∆E 8.34) of rocket leaves. In addition, OA treatments decreased the vitamin C losses and chlorophyll degradation. In conclusion, rocket leaves could be stored at 0 °C for 8–9 days with 1 mM OA treatment and 6 days with the control treatment and at 10 °C for 6–7 days with 1 mM OA treatment and 4 days with the control (C) treatment with a minimum quality loss under MAP conditions. [ABSTRACT FROM AUTHOR]

Nutritious foods like vegetables and fruits have therapeutic potential in treatment of many human diseases. Several bioactive compounds have been extracted from plants and fruits, especially antioxidants which aid protect against oxidative stress. This study was conducted on Albino Wistar rats to investigate the antioxidant effect of Eruca sativa on the hepatic and renal profile damaged by xylene. Seventy rats were divided into seven equal groups and treated by gavage for 30 days as follows: Control group (C), group (CO), positive control group (RE) was received 350 mg / Kg bw of Eruca sativa aqueous extract (ESAE), 2 toxic groups X1 and X2 were treated with xylene at two doses 400 and 800 mg / Kg bw and 2 combination groups REX1 and REX2 were treated with ESAE (350 mg/kg bw) combined with 400 and 800 mg/Kg xylene respectively. The results revealed that oral treatment with xylene (X1 and X2) caused hepatic and renal dysfunction, which was manifested by a significant elevation of AST, ALT, ALP, urea, uric acid and creatinine plasma levels. Additionally, a significant decrease in GSH levels and GPx activity accompanied with an increase in MDA levels were noted due to the exposition of xylene. However, animals that received ESAE with xylene (REX1 and REX2) showed an adjustment of these perturbations, by a significant decrease in the levels of AST, ALT, ALP, urea, uric acid, creatinine and MDA. As well as an increase in GSH levels and GPx activity compared to X1 and X2 groups. The histological profile of toxic groups showed pathological changes in liver and kidney tissue were characterized by sinusoidal and tubular dilatation, hemorrhage, hepatocyte necrosis and glomerular degeneration, the effect of ESAE was effective in modified these damages into semi normal. To conclude, xylene administration induced hepatotoxicity and nephrotoxicity in wistar rats, while the combination of this solvent with rocket aqueous extract (Eruca sativa) attenuated this toxicity thanks to its antioxidant property. [ABSTRACT FROM AUTHOR]

Soil salinity can have various negative consequences on agricultural products, from their quality and production to their aesthetic traits. In this work, the possibility to use salt-affected vegetables, that otherwise would be discarded, as a source of nutraceuticals was explored. To this aim, rocket plants, a vegetable featuring bioactive compounds such as glucosinolates, were exposed to increasing NaCl concentrations in hydroponics and analysed for their content in bioactive compounds. Salt levels higher than 68 mM produced rocket plants that did not comply with European Union regulations and would therefore be considered a waste product. Anyway, our findings, obtained by Liquid Chromatography-High Resolution Mass Spectrometry, demonstrated a significant increase in glucosinolates levels in such salt-affected plants. opening the opportunity for a second life of these market discarded products to be recycled as glucosinolates source. Furthermore, an optimal situation was found at NaCl 34 mM in which not only were the aesthetic traits of rocket plants not affected, but also the plants revealed a significant enrichment in glucosinolates. This can be considered an advantageous situation in which the resulting vegetables still appealed to the market and showed improved nutraceutical aspects. [ABSTRACT FROM AUTHOR]

Eruca sativa is a leafy vegetable widely consumed fresh in salads and recognized for the presence of bioactive compounds, such as glucosinolates (GLS) and flavonols. This plant is traditionally cultivated in soils but adapts well to soilless cultures, such as hydroponics and aquaponics. However, despite the good results in the literature on E. sativa cultivation in soilless systems, the influence of the culture systems and harvest time on the specialized metabolite-based chemical composition of E. sativa leaves is not entirely understood. Based on the above, this study aimed to evaluate the specialized metabolite composition of three different cultivation types, i.e., using soil (SCS), nutrient film technique (NFT)-based hydroponic (HCS), and aquaponic (ACS) culture systems, along three growing cycles, and collected at two commercial harvest times, i.e., 21 days after transplanting (DAT) to get early plant material, namely "baby leaf", and 42 DAT as the traditional harvest time. The chemical composition was obtained by liquid chromatography coupled with mass spectrometry (LC-MS), and multivariate statistics supported the analysis of the whole dataset. The SCS was characterized to promote an important accumulation of two antioxidant flavonols, i.e., (kaempferol and isorhamnetin diglucopyranosides) in young leaves (21 DAT). The hydroponically-grown plants exhibited a smaller number of various compounds. The ACS-cultivated leaves accumulated indole-containing glucosinolates and a marker associated with harvest time, spirobrassinin, a cruciferous oxindoline phytoalexin. These findings constitute the first report of those compounds relevantly accumulated by the effect of soilless cultures and a starting point for further studies related to the metabolite regulation of E. sativa under hydroponics and aquaponics. [ABSTRACT FROM AUTHOR]

Soil salinization become worse in the last decades, leading to reduced crop yields, especially in the Mediterranean basin. Eruca sativa is a common species cultivated in this area with remarkable economic importance. This study aimed at investigating the effect of salinity on this plant, focusing on (i) seedling development in terms of variations in germination and growth parameters and (ii) anatomical and ultra-structural changes in the morphology of cotyledons. For this reason, seeds were treated with different salinity levels ranging from 137 to 548 mM NaCl. Seed germination was delayed by all the concentrations tested, but only above 137 mM seedling growth was impaired. Results showed a high occurrence of lipid bodies within the mesophyll cells of cotyledons of seedlings exposed to salt concentrations above 137 mM, suggesting an impairment in lipid mobilization caused by salinity during plant development. The cotyledons of treated seedlings showed reduced intercellular spaces and ultrastructural changes in chloroplasts and peroxisomes. Moreover, salt-induced autophagic processes were present in samples grown at the highest NaCl levels. Interestingly, at 137 mM NaCl, seedlings showed the highest values of mesophyll thickness and fresh weight, implying a possible mechanism of salt adaptation during germination. [ABSTRACT FROM AUTHOR]

A field experiment was conducted during the 2018-2019 season in one of the private fields in Karma City, Anbar Governorate, Iraq to study the effects of organic fertilizer levels (0, 5 and 10 tons ha-1) and spraying with amino acid arginine at concentrations of 0, 100, 200 mg L-1 on growth and also yield characteristics in addition to some medicinally-active compounds in watercress seeds, Eruca sativa Mill. The study factors were arranged as splitplot experiment according to the randomized complete block design (RCBD), with three replications. According to the results, the fertilizer level at 10 tons ha-1 exhibited a significant elevation in the characteristics of growth, yield, oil rate (%), and seed contents of glucosinolate glycoside and Astragalin alkaloid, whereas spraying arginine at 200 mg L-1 showed significant differences in the characteristics of yield, oil rate (%), and seed content of glucocinolate glycoside and Astragalin alkaloid. In addition, the interaction of the studied characteristics displayed a significant effect. So that, the plants given 10 tons ha-1 of organic fertilizer under the influence of spraying with 200 mg L-1 arginine, revealed the highest rate of 1000-seed weight, oil rate (%), in addition to the seed content of glucocinolate glycoside and Astragalin. [ABSTRACT FROM AUTHOR]

Rocket is the common designation for two baby-leaf salad crops of the Brassicaceae family: Eruca sativa (L.) Cav., usually referred to as annual garden rocket, and Diplotaxis tenuifolia (L.) DC. commonly named to as perennial wild rocket. E. sativa is used for human consumption since antiquity. However, the growing consumer preference for D. tenuifolia is being accompanied by the fast increase in its production area and commercialization of new cultivars. Nevertheless, the worldwide number of wild rocket accessions maintained in germplasm collections is very reduced, the solution for which situation the project "REMIRucula" intends to contribute, establishing a germplasm collection at the INIAV, Oeiras, Portugal. Herein, we report on the establishment via next generation sequencing (NGS) of the first genome assembly of D. tenuifolia and the identification of specific single sequence repeat (SSR) and single nucleotide polymorphisms (SNP) loci for the establishment of specific DNA-markers for this species. A representative set of 87 D. tenuifolia and 3 E. sativa accessions were assessed by 5 SSR and 9 SNP-CAPS markers, allowing a drastic discrimination between both species and the establishment of unequivocal molecular fingerprints for the analyzed accessions. The non-discrimination within six pairs and one trio of D. tenuifolia accessions is discussed. [ABSTRACT FROM AUTHOR]

Fusarium graminearum is the most important causal agent of head blight in wheat, and stalk and ear rot in maize. A field experiment was conducted to investigate the effect of incorporation of Brassicaceae cover crops on Fusarium graminearum in a wheat-maize rotation. Five species belonging to Brassicaceae (Brassica juncea, Eruca sativa, Raphanus sativus, B. carinata, B. oleracea var. caulorapa L.) were used in the field experiment to investigate their potential to suppress F. graminearum inoculum in soil, disease incidence in maize and to reduce subsequent mycotoxin contamination in maize. Brassica juncea was found to contain the highest glucosinolate concentration in shoots (31 µmol g−1). Severity of ear rot and stalk rot in maize was not significantly reduced in the amended plots. Incorporation of R. sativus 'Terranova' significantly decreased the amount of F. graminearum DNA by 58% compared with the cultivated fallow treatment, however the DNA concentration was not significantly different to fallow uncultivated. Fusarium graminearum DNA and deoxynivalenol in maize was 50% lower after incorporation of B. oleracea var. caulorapa L. compared to after fallow treatment but the difference was not significant. The brassica crops used in the present field experiment were not effective in suppressing F. graminearum, therefore further studies to optimise the current approach are recommended. [ABSTRACT FROM AUTHOR]

Copyright of Revista em Agronegócio e Meio Ambiente is the property of Revista em Agronegocio e Meio Ambiente and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Egyptian Journal of Histology is the property of Egyptian Journal of Histology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Fusarium graminearum is a globally important cereal pathogen, causing head blight in wheat, resulting in yield losses and mycotoxin contamination. Currently, triazole fungicides are used to suppress Fusarium graminearum, however, the declining effectiveness of triazoles and concerns over the safety of pesticides have led to the pursuit of safe alternative crop protection strategies such as biofumigation. In the present study, species belonging to Brassicaceae (Brassica juncea, Raphanus sativus, Eruca sativa) were assessed for their biofumigation potential against F. graminearum and the glucosinolate profile of the brassicas was determined. In Petri dishes, mycelial plugs of Fusarium graminearum were exposed to frozen/defrosted leaf discs of brassicas collected at early-leaf, stem-extension, and early-bud stages. Additionally, F. graminearum inoculum was incubated in soil amended with chopped tissues of brassicas in a closed jar experiment. Glucosinolate analysis of the leaf tissue of brassicas revealed that the total glucosinolate concentration of B. juncea 'Brons' increased with advancing growth stage (24.5–51.9 µmol g−1). Brassica juncea leaf discs were effective against mycelial growth, while the sinigrin content in the leaf tissue corresponded to the level of suppression. At the stem-extension and early-bud stages, B. juncea 'Brons' showed 87–90% suppression with four leaf discs, and 100% suppression with eight leaf discs. Brassica juncea 'Caliente Rojo' leaf discs collected at the stem-extension stage showed 94% inhibition with eight discs. In the closed jar experiment, each brassica species significantly suppressed F. graminearum inoculum by 41–55%. The findings suggest that the brassica species investigated in the present study could be effective in reducing the inoculum of F. graminearum in soil prior to cereal production. [ABSTRACT FROM AUTHOR]

To investigate the potentiality of Eruca sativa (rocket) to be enriched in selenium (Se) and, thus, to promote human health through consumption, a pot experiment was designed. Two rates, 5 and 10 mg/kg soil, of either selenite or selenate sodium salts were applied to appropriate pots, each filled with 1 kg of calcareous soil. Rocket seedlings were transplanted and grown in these pots, and to half of the pots the biostimulant Actiwave was added. Twelve weeks later, the plants were harvested and Se concentrations determined in shoots and roots. Plant growth characteristics were measured and plants biometrics were assessed by soil plant analyses development (SPAD), normalised difference vegetation index (NDVI) and normalised difference red edge (NDRE). Sulfur (S) and phosphorus (P) concentrations in plant samples were also determined to discuss possible interactions among the three elements. The highest Se concentration of 1070.5 mg/kg dry weight (DW) was observed for the high selenate rate without biostimulant, placing rocket in the group of Se hyperaccumulator plants. Toxic effects were recorded for the plants that received the high selenate rate, whereas no toxicity symptoms were observed for either selenite rate. According to Se concentrations in controls, biostimulant application significantly suppressed Se uptake and significantly increased S and P uptake. The same negative biostimulant effect on Se concentration in plants was clear in selenate treatments. When the results were expressed as total uptake (mg/pot), positive correlations among Se, S and P were found for selenate treatments, whereas for selenite treatments, the opposite was observed. Impressively, 1.6 mg Se/pot on a DW basis was accumulated in rocket shoots in the low selenate-rate without biostimulant treatment, corresponding to approximately 30% of the added 5 mg of selenate. Selenium is an essential element for humans and animals. This study reports on the enrichment of selenium in rocket plants and the results showed that when selenium is added as selenate, the plants accumulate high amounts of selenium. Although further research is needed to optimise the selenate application rate so as to avoid leaching and subsurface water contamination, it seems feasible to produce a vegetable of high nutritional value for human consumption without negatively affecting the environment. [ABSTRACT FROM AUTHOR]

The associated species to Senecio glaucus in the newly reclaimed desert near to Wadi El-Assiuty of the Eastern Desert at Assiut Governorate, Egypt were investigated over two years (2018 and 2019). The allelopathic potential of S. glaucus against two crop plants (Triticum aestivum and Eruca sativa) was also evaluated. Floristically, 65 species within 59 genera and 22 families were recorded (21 perennials and 44 annuals). Four families Asteraceae, Poaceae, Brassicaceae and Fabaceae captured about 54% of this flora. Therophytes are the predominant life form followed by phanerophytes. Chorological analysis revealed that cosmopolitan and Saharo-Arabian elements constitute the main bulk of the total flora of the studied area. Cluster analysis yielded four distinct groups. Stands of group A characterized by the absence of S. glaucus and highest species diversity. Stands of group B, C and D included the associated species of S. glaucus and characterized by the dominance of cosmopolitans, grasses and salt tolerant species. The ordination techniques revealed the significant difference between group A and the others and showed the strong correlation between its flora with organic matter. The other three groups were highly correlated with coarse soil texture and total salts. Thirteen species were absent from stands of groups B, C and D in which S. glaucus was dominant. Phytochemical analysis of S. glaucus showed the prevalence of total phenolics and flavonoids in its shoots, while terpenoids were the highest in its flowers. All fractions of the plant components completely prevented germination of E. sativa seeds but exerted different magnitude of inhibition on germination of T. aestivum caryopses (especially with MeOH, capitula and shoot extracts). Estimation of phytotoxicity revealed that aqueous, ethyl acetate and capitula extracts significantly inhibited the germination of wheat caryopses and reduced the elongation rates of hypocotyl and epicotyl, and hence the whole length of seedlings. [ABSTRACT FROM AUTHOR]

Worldwide, the primary problem today is the proliferation of cancer and secondary bacterial infections caused by biofilms, as they are the principal causes of death due to the lack of effective drugs. A great deal of biological activities of silver nanoparticles (AgNPs) have made them a brilliant choice for the development of new drugs in recent years. The present study was conducted to evaluate the anticancer, antibacterial, anti-QS, and antibiofilm effects of AgNPs synthesized from Eruca sativa (E. sativa) leaf extract. The ultraviolet–visible (UV–Vis) spectra showed a peak of surface plasmon resonance at 424 nm λmax, which corresponded to AgNP formation. The Fourier transform infrared spectroscopy (FT-IR) confirmed that biological moieties are involved for the development of AgNPs. Moreover, transmission electron microscopy (TEM) analyses confirmed the spherical shape and uniform size (8.11 to 15 nm) of the AgNPs. In human lung cancer cells (A549), the anticancer potential of AgNPs was examined by the MTT [3-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, scratch assay, and invasion assay. The results indicated that AgNPs inhibit the migration of A549 cells. The synthesized AgNPs showed MIC values of 12.5 µg/mL against Chromobacterium violaceum (C. violaceum) and 25 µg/mL against Pseudomonas aeruginosa (P. aeruginosa), which demonstrated their antibacterial abilities. Biological compounds that disable the QS system are being investigated as potential strategies for preventing bacterial infections. Thus, we analyzed the potential effectiveness of synthesized AgNPs in inhibiting QS-regulated virulence factors and biofilm formation in both strains of bacteria. In C. violaceum, the synthesized AgNPs significantly inhibited both violacein (85.18% at 1/2 × MIC) and acyl homoserine lactone (78.76% at 1/2 × MIC). QS inhibitory activity was also demonstrated in P. aeruginosa at a sub-MIC concentration (1/2 × MIC) by a reduction in pyocyanin activity (68.83%), total protease (68.50%), LasA activity (63.91%), and LasB activity (56.40%). Additionally, the exopolysaccharide production was significantly reduced in both C. violaceum (65.79% at 1/2 × MIC) and P. aeruginosa (57.65% at 1/2 × MIC). The formation of biofilm was also significantly inhibited at 1/2 × MIC in C. violaceum (76.49%) and in P. aeruginosa (65.31%). Moreover, a GC–MS analysis confirmed the presence of different classes of bioactive phytochemical constituents present in the leaf extract of E. sativa. On the basis of our results, we conclude that biologically synthesized AgNPs showed numerous multifunctional properties and have the potential to be used against human cancer and bacterial biofilm-related infections. [ABSTRACT FROM AUTHOR]

The use of contaminated water to irrigate crop plants poses a risk to human health from the bioaccumulation potential of microcystins (MCs) in the edible tissues of vegetable plants. The main objective of this study is to determine the concentration of total microcystins (MC-LR and MC-RR) in leafy green plants (Lactuca sativa L. var. longifolia and Eruca sativa) that have previously been irrigated with polluted water. Integrated water samples were collected by cleaned plastic bottles at a depth of about 30 cm from one of the sources of water used to irrigate agricultural lands for crop plants. At the same time, samples from plants were also collected because this water from the lake farm is used for the irrigation of surrounding vegetable plants such as Lactuca sativa L. var. longifolia and Eruca sativa. The dominant species of cyanobacteria in water samples are Microcystis aeruginosa (Kützing) and Oscillatoria limnetica Lemmermann, which were detected with an average cell count 2,300,000 and 450,000 cells/mL, respectively. These two dominant species in water produced two MCs variants (MC-LR, -RR) that were quantified by high-performance liquid chromatography (HPLC). Dissolve and particulate MCs were detected in the irrigation waters by HPLC with concentrations of 45.04–600 μg/L. MCs in the water samples exceeded the WHO safety limit (1 μg/L) of MC in drinking water. In addition, the total concentration of Microcystin in Lactuca sativa L. var. longifolia and Eruca sativa were 1044 and 1089 ng/g tissues, respectively. The estimated daily intake (EDI) of microcystins by a person (60 kg) consuming 300 g of fresh plants exceeded the total daily intake guidelines (0.04 μg kg−1 body weight) for human food consumption. According to the findings of this study, irrigation water and plants used for human consumption should be tested for the presence of MCs regularly through critical and regularly monitored programs to prevent the accumulation and transfer of such toxins through the food web. [ABSTRACT FROM AUTHOR]

To investigate the potentiality of Eruca sativa (rocket) to be enriched in selenium (Se) and, thus, to promote human health through consumption, a pot experiment was designed. Two rates, 5 and 10 mg/kg soil, of either selenite or selenate sodium salts were applied to appropriate pots, each filled with 1 kg of calcareous soil. Rocket seedlings were transplanted and grown in these pots, and to half of the pots the biostimulant Actiwave was added. Twelve weeks later, the plants were harvested and Se concentrations determined in shoots and roots. Plant growth characteristics were measured and plants biometrics were assessed by soil plant analyses development (SPAD), normalised difference vegetation index (NDVI) and normalised difference red edge (NDRE). Sulfur (S) and phosphorus (P) concentrations in plant samples were also determined to discuss possible interactions among the three elements. The highest Se concentration of 1070.5 mg/kg dry weight (DW) was observed for the high selenate rate without biostimulant, placing rocket in the group of Se hyperaccumulator plants. Toxic effects were recorded for the plants that received the high selenate rate, whereas no toxicity symptoms were observed for either selenite rate. According to Se concentrations in controls, biostimulant application significantly suppressed Se uptake and significantly increased S and P uptake. The same negative biostimulant effect on Se concentration in plants was clear in selenate treatments. When the results were expressed as total uptake (mg/pot), positive correlations among Se, S and P were found for selenate treatments, whereas for selenite treatments, the opposite was observed. Impressively, 1.6 mg Se/pot on a DW basis was accumulated in rocket shoots in the low selenate-rate without biostimulant treatment, corresponding to approximately 30% of the added 5 mg of selenate. [ABSTRACT FROM AUTHOR]

The current experiment was intended to investigate the hepatoprotective, immunostimulant activity, behavioural and anxiety responses of mice supplemented with Eruca sativa aqueous extract (ESAE) at a dose of 1% in drinking water in healthy and thioacetamide (TAA) intoxicated male mice. Eighty male albino mice were divided into four treatments of 20 mice each, with two replicates. The T1 considered as control group, T2 was the TAA intoxicated group, T3 was supplemented with ESAE only in drinking water for 3 weeks, and T4 supplemented with both ESAE for 3 weeks and TAA in the last 3 days in drinking water. The effect of ESAE was assessed by estimation of erythrogram, leukogram, platelet count, N/L ratio, total protein, albumin, globulin, and A/G ratio, and by evaluating the activity of liver enzymes such as aspartate transaminase (AST) and alanine transaminase (ALT) as well as the behavioural responses and anxiety of mice were also recorded. ESAE administration markedly prevents the elevation of plasma liver enzymes after oral administration of TAA. The mice supplemented with ESAE (T3) had higher mean values of total protein, globulin, with lower neutrophils count and A/G ratio compared to the control group (T1) and TAA intoxicated mice (T2), indicating that ESAE has immunostimulant and hepatoprotective activity. The mice supplemented with ESAE (T3) groomed, scratched, and sniffed significantly more than mice of T2, with significantly less resting and sleeping. Regarding the elevated plus maze results; when comparing mice of T1 and T2 with other treatments, the mice of T3 and T4 showed a huge (P<0.05) decline in the quantity of closed arms entries, with a relating increase in the quantity of open arms entries. It is concluded that a daily oral dose of 1% ESAE improves mouse activity, reduces anxiety, and has immunostimulant and hepatoprotective activity. [ABSTRACT FROM AUTHOR]

As a versatile cruciferous species, Eruca sativa is widely cultivated, but in some areas, it has become an invasive weed. There are few studies on its seed dormancy and soil seed bank. This research examined seed dormancy, germination, and dynamics of the soil seed bank of E. sativa , with a view to provide support for its prevention and control. We tested the effects of temperature, light, storage, water, and salinity stress on seed germination and burial depth on seedling emergence of E. sativa. Dynamics of the soil seed bank were determined with a 24 month in situ seed-burial study. Seeds of E. sativa can germinate in a temperature range of 5–35°C; moreover, they exhibited non-deep physiological dormancy (NDPD) at maturity, which can be broken by dry storage or exposure to low temperature in winter. Germination of E. sativa seeds was sensitive to water and salinity stress, and most seeds did not germinate at -0.3 MPa. When buried in soil in the field, seeds exhibited an annual dormancy/non-dormancy cycle and formed at least a short-term persistent soil seed bank. Seeds buried deeper than 5 cm can hardly emerge. Seeds of E. sativa have a wide germination temperature range and exhibited dormancy cycling, which promotes the formation of a persistent soil seed bank and enables it to better adapt to the harsh low-temperature climate of the Qinghai-Tibet Plateau. No-tillage would be a good management strategy for this species. [ABSTRACT FROM AUTHOR]

Germination models are quite helpful in predicting emergence times, dormancy periods, and their applications in crop management. This study investigated the germination behaviors of Eruca sativa Mill. in response to fluctuations in temperatures (Ts) and water potentials (ψs). Germination percentage (GP) increased 95% with rising temperature within the range of 20–30 °C, and decreased 25% at 5 °C. Moreover, each ψ and T resulted in a decrease in GP as ψ decreased. Further, we noted that the θT1 value was substantially high at 30 °C and in (0 MPa), whereas the θT2 value was maximum at 10 °C (−0.02 MPa) and it decreased with decreasing Ψ. The maximum hydrothermal time constant (θHTT) and hydrotime (θH) values were obtained at 10 and 30 °C, respectively. In addition, a linear increase in the GR(g) pattern was observed at Tb and a decrease below the To. The calculated cardinal Ts was 5 °C for the base T, and 30 °C for both the optimum and ceiling T. The germination characteristics were higher at 30 °C having (0 MPa). Therefore, using cardinal temperatures, germination results, and the hydrothermal time model (HTT) could reveal the independent and interactive impacts of both T and the Ψ on the response of seed germination subjected to diverse environmental conditions. [ABSTRACT FROM AUTHOR]

Use of green agronomic techniques for plant development and crop protection is essential for environmental sustainability. The current research investigates a more efficient and long-term technique of manufacturing silica nanoparticles (SiO2 NPs) from agricultural waste (sugarcane bagasse and corn cob). SiO2 NPs were synthesized by calcinations of waste residues in muffle furnace with varying temperatures (400–1000 °C)/2 h in the present of static air. Field emission scanning electron microscopy (FESEM), Fourier transmission infrared spectroscopy (FTIR), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX) were used to characterize SiO2 NPs and assessed for their antifungal activity simultaneously investigated the effects of various concentrations of produced SiO2 NPs on Eruca sativa (E. sativa) physiological and biochemical. With SiO2 NPs treatment at 1000 µg L−1 concentration, the seed germination rate was found to be up to 95.5%, and growth characteristics were enhanced compared to control. Accordingly, the ones treated with SiO2 NPs grew better than the control ones. The treatment of plant with SiO2 NPs (500 μg L−1) increased the protein content by 14.8 mg g−1, and chlorophyll level was also increased by 4.08 mg g−1 in leaves compared to untreated plant. Disc diffusion experiment was conducted to test the efficiency of SiO2 NPs against Fusarium oxysporum and Aspergillus niger for antifungal activities. Highest mycelia growth inhibition was obtained with 73.42% and 58.92% for F. oxysporum and A. niger, respectively. The result shows that the SiO2 NPs have a favorable effect on E. sativa growth and germination, enhancing plant production which helps to improve the sustainable agriculture farming and acting as a possible antifungal agent against plant pathogenic fungi. [ABSTRACT FROM AUTHOR]

Copyright of Agricultural Research in the Tropics / Pesquisa Agropecuária Tropical is the property of Pesquisa Agropecuaria Tropical and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Paraquat (PQ) causes oxidative stress, the main source of damage in plants subjected to adverse environmental factors. Sodium nitroprusside (SNP), a signaling molecule, alleviates oxidative damage. The present study was carried out to investigate the role of exogenous SNP in the amelioration of PQ-mediated oxidative stress effects on Eruca sativa plantlets cultured in MS basal media. Firstly, MS medium supplemented with 6-BA was found to be the best basal medium for seed germination. Then, a rapid micropropagation protocol was designed to produce E. sativa plantlets by using nodal segments as explants, and 0.25 mg/L 6-BA in combination with 0.1 mg/L IBA was found to be the most favorable for shoot proliferation of E. sativa. Four weeks old plants were applied with or without SNP (100 μM) and exposed to oxidative stress induced by 2.5 μM PQ. The SNP application decreased membrane damage, hydrogen peroxide, and proline contents, and increased relative water, pigments, ascorbate and total phenolic contents, and some antioxidant enzyme activities in seedlings under PQ stress compared to PQ stress alone. These results suggested that exogenous SNP could protect E. sativa plantlets propagated in vitro with PQ stress through modulation of proline and phenolics biosynthesis and antioxidant defense system. [ABSTRACT FROM AUTHOR]

Copyright of Canadian Journal of Plant Science is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Salad rocket (Eruca sativa), which is becoming increasingly important, comprises a wide variety of phytochemicals that promote health, such as carotenoids, fibers, glucosinolates (GLs), polyphenols, and ascorbic acid (AsA). Variations in the nutritional components of a rocket cultivar and the effects of different organic matter on biochemical changes under salt stress were examined herein. The response of salad rocket (Eruca sativa.) to applications of farmyard manure, humic acid, or vermicompost, as well as water irrigation salinity at 8 dS m-1 was evaluated under greenhouse conditions using plastic pots containing 12 L of loamy sand. For each experiment, 5 treatments were performed, as follows: 1) control [C: NaCl-free], 2) salt treatment [S: irrigation with solution contained 8 dS m-1], 3) salt treatment + cow manure [SM: irrigation with solution contained 8 dS m-1 + farmyard manure 136 g pot-1], 4) salt treatment + HA [SH: irrigation with solution contained 8 dS m-1 + 100 ppm HA], 5) salt treatment + vermicompost (worm casting) [SV: irrigation with solution contained 8 dS m-1 EC + 13.6 mg pot-1]. The experimental treatments were arranged in a randomized plot design with four replicates. At the experiment's conclusion, the malondialdehyde (MDA), total phenolic, flavonoid, AsA, total GLs contents, and antioxidative enzyme activities [ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD)] were determined in the rocket leaves. The results revealed that the salt stress caused decreased photosynthetic pigments like total chlorophyll (Chl), Chl-a, Chl-b, and carotenoid contents, as well as total the GLs and phenolic contents, while total flavonoid, AsA, and SOD, CAT, APX, and GR activities showed an increase. The organic matter applications provided tolerance at different levels, and following the aforementioned procedures, there were significant increases (19%-134%), but the MDA content was reduced by 47%. Moreover, the application of organic matter increased the total GLs and antioxidative enzyme activities of APX, CAT, GR, and SOD under salt stress (21-424% increase). The results supported the application of organic matter to increase the defense system of rocket plant, enabling it to tolerate the negative effects induced by salinity. In addition, these applications contributed to the antioxidative defense system components in salad rocket. [ABSTRACT FROM AUTHOR]

Copyright of Revista Caatinga is the property of Revista Caatinga and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Caatinga is the property of Revista Caatinga and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The increase in the cultivation area of cruciferous oilseed crops and the use of short crop rotation has resulted in the enhanced spread of several major pests in Northern latitudes. There is currently limited information about incidence and severity of Alternaria black spot disease (Alternaria brassicae) on the main oilseed crop, spring oilseed rape (Brassica napus), in the Northern Baltics. Thus, spring oilseed rape and five alternative cruciferous oilseed crops were selected and their resistance to black spot disease was evaluated in field conditions during two growing seasons. We hypothesized that spring oilseed rape is more susceptible to Alternaria black spot disease than other alternative cruciferous oilseed crops. Both growing seasons were warmer and drier compared to the long-term average, and were thus suitable for A. brassicae development and assessments. In both years, incidence of Alternaria black spot infection was recorded on all cruciferous species, yet the disease development differed considerably among the crops. During both growing seasons, black mustard (B. nigra) plants were the most infected. Based on our observations during warm growing seasons we conclude that alternative oilseed crops such as Sinapis alba, Eruca sativa and Raphanus sativus are more resistant to the Alternaria black spot infection than the traditional oilseed crops and thus, possess a great potential to grow with limited chemical disease control in Northern Baltic conditions. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Science & Technology (2008-8787) is the property of Tarbiat Modares University Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to make possible the agricultural application of the wastewater treatment plant (WTP) sludge, it is necessary to characterize and classify this waste taking into account mainly the possibility of the existence of compounds that configurate toxicities to the sludge and the presence of pathogenic agents, considering they are risk factors to the public health and environment. However, the legal texts about this reuse do not mention the need of toxicity essays that could complement the proposed characterizations and assure the reuse. This way, this investigation presents a toxicity study of the sanitary WTP sludge, as well as physic-chemical, microbiological, and parasitological analyses. For this purpose, a sample of the studied sludge was submitted to microbiological and parasitological essays. Next, the sample of the sludge was solubilized in accordance with the Brazilian Association for Technical Standards, being submitted the solubilized extract to physic-chemical analyses. After that, the ecotoxicology essays were performed with the organisms Daphnia magna and Eisenia fetida (worm) and with the herbaceous plant Eruca sativa (arugula). The presented toxicity essay results on the tested organisms indicate satisfactory and non-toxic values of mortality, mass gain, fertility, survival, and germination, revealing positive potential to the sludge use in agriculture. [ABSTRACT FROM AUTHOR]

This experiment was conducted on 120 unsexed Cobb chicks of one day old for 28 days. Chicks were randomly divided into 4 groups (30 chicks each) and each group consists of two replicates (15 chicks each). All groups was fed using a basal diet, but the first group drunk water from the tap as a control, while the other drunk water with extract of Eruca Sativa leaves (0.25, 0.50 and 0.75ml/Liter) respectively. This study aimed to determine the effect of extraction of Eruca Sativa leaves on broiler performance, some blood parameters and immune response to Newcastle virus. The result showed that the supplementation of the broiler diet with extraction of Eruca sativa especially of levels (0.25 and 0.50 ml/L) significantly improve the fnal body weight (P≤0.05). The weight gain also significantly improved (P≤0.05) but, there were no significantly changes in the weight of internal organs and blood pictures (WBCs, RBCs, Hb and PCV respectively). The antibody titer against the Newcastle virus found to be significantly increased (P≤0.05). [ABSTRACT FROM AUTHOR]

The effect of light emitting diode (LED) light sources with different spectra on Eruca sativa Mill., cv. Dikovina plant development was investigated. Experiments were conducted with four types of LED lamps of our own assembly: solar box (SB), red-green-blue (RGB), red-blue (FS), and warm white (WW) used as a control with an energy ratio in the blue-green-red range of 14% : 48% : 38%. Spectra of SB (26% : 41% : 33%), RGB (32% : 19% : 49%), and FS (27% : 11% : 62%) lamps were used to obtain nine different types of lighting ensuring modification of spectra in the course of growing. The plants exposed to FS-RGB, SB-FS, and RGB-SB light sources were characterized with the greatest values of the shoot fresh weight. Transfer of plants to SB resulted in a decrease in development of the plants root system. Transformation of leaf mesostructure after transfer to SB showed that light conditions therein promoted the formation of leaf structure with lower membrane indexes and deformed mesophyll cells. At the same time, cultivation of plants for 2 weeks in SB with subsequent transfer to FS for 4 more weeks stimulated development of internal assimilatory surface of the leaf with the greatest integral indexes of the outer membrane surface of leaf cells (Ames/A) and chloroplasts (Achl/A) as compared with other treatments. As a result, SB-FS plants produced maximal dry shoots and roots biomass. Without alteration of the lighting pattern, plants from box FS were inferior to control plants (module WW) in parameters of leaf mesostructure and ranked second among other types of treatment. The greatest accumulation of P and Ca was observed in FS-RGB where a maximal percentage of shoots and roots dry matter and the greatest concentration of ascorbic acid were found. Transfer of plants from other boxes to FS caused a decrease in accumulation of macroelements in E. sativa plants. Thus, the dynamic lighting applied for growing of E. sativa plants affected their characteristics and could be used for optimization of plant cultivation. [ABSTRACT FROM AUTHOR]