1. Granulocyte antibodies in male blood donors: can they trigger transfusion-related acute lung injury?
- Author
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Nguyen, Xuan‐Duc, Schulze, Torsten J., Bugert, Peter, Lauber‐Härtl, Stephanie, Schulz‐Linkholt, Monika, González‐Schulze, Karen, Reil, Angelika, Dengler, Thomas, Panzer, Simon, Seifried, Erhard, Nguyen, Xuan-Duc, Lauber-Härtl, Stephanie, Schulz-Linkholt, Monika, and González-Schulze, Karen
- Subjects
GRANULOCYTES ,BLOOD donors ,BLOOD transfusion ,ERYTHROCYTES ,LUNG injuries ,AUTOANTIBODIES ,ENZYME-linked immunosorbent assay ,FLOW cytometry ,MEDICAL screening ,NEUTROPHILS ,HLA-B27 antigen ,DISEASE incidence - Abstract
Background: White blood cell-associated antibodies can lead to transfusion-related acute lung injury (TRALI). Female donors with a history of pregnancies have been identified as a main cause for these antibodies. Male or female donors without a history of pregnancy are considered as safe donors.Study Design and Methods: Following the identification of two TRALI cases associated with blood products from male donors, we investigated the frequency of granulocyte-specific and human leukocyte antigen (HLA) antibodies in the entire blood donor population using a high throughput automated flow-cytometry-based granulocyte immunofluorescence test (Flow-GIFT). We investigated sera from 14,343 whole blood donors (female, n = 6974, 48.7%; male, n = 7369, 51.3%) using automated Flow-GIFT. Of the female blood donors, 60.4% had a history of pregnancy. Positive sera were retested by the standard granulocyte immunofluorescence test and granulocyte agglutination test. For the detection of HLA Class I and II immunoglobulin G antibodies, we used a commercial screening enzyme-linked immunosorbent assay.Results: We detected in 924 (21.9%) of the 4212 females with a history of pregnancy antibodies against granulocyte antigens (n = 62, 1.5%), HLA Class I and/or II antigens (n = 864, 20.5%). Notably, in 3.5% (n = 96) of 2762 females without a history of pregnancy and in 2.1% (n = 154) of 7369 males antibodies against granulocyte antigens (n = 13, 0.47% and n = 45, 0.6%), HLA Class I and/or II (n = 83, 3% and n = 109, 1.4%, respectively), were also detected.Conclusion: Human neutrophil antigen antibodies are rare in male and females without a history of pregnancy compared to females with a history of pregnancy, but their relevance is not negligible. [ABSTRACT FROM AUTHOR]- Published
- 2018
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